A constant temperature storage device for polysialic acid fermentation medium

Abstract

The invention discloses a constant temperature storage device for polysialic acid fermentation culture medium, which relates to the technical field of culture medium storage; the device comprises a storage container, wherein an internal array of the storage container is provided with a mounting table, an array of placement holes is provided on the mounting table, and a positioning mechanism is provided in the placement hole; the positioning mechanism comprises a mounting ring provided in the placement hole, a ball portion is connected to the mounting ring by a ball pair, and a culture tube is detachably provided in the ball portion; a supporting cylinder is connected to the bottom of the ball portion, and the supporting cylinder is deflected to shake the culture tube; a transfer mechanism is provided inside the storage container for transferring and placing the culture tube; an adding mechanism is provided at the bottom end of the storage container for injecting additives into the culture tube; a blocking mechanism is provided on the side of the storage container, and when the culture tube is shaken, the blocking mechanism blocks the open end of the culture tube; the invention is rich in functions, enhances the convenience of storage, can supplement the addition of additives, realizes the shaking of the culture tube, and reduces the difficulty of operation.

Description

Technical Field

[0001] The invention relates to the technical field of culture medium storage, and in particular to a constant temperature storage device for polysialic acid fermentation culture medium. Background Art

[0002] Polysialic acid is a complex carbohydrate compound that is commonly found in certain bacteria and fungi. It is a polysaccharide composed of sialic acid units and is widely used in biomedical research and industrial production. Polysialic acid plays important roles on the cell surface, including regulating cell-cell interactions and protecting cells from attacks by the immune system. It is important in neural development, cancer research, and cell signaling; in industrial production, polysialic acid is used as an excipient for vaccines, drug delivery systems, and biomaterials. The preparation and use of polysialic acid culture medium is mainly used for the research and production of polysialic acid microorganisms or cells; in the current preparation of polysialic acid fermentation culture medium, the storage of the culture medium is a necessary process, and changes in the storage environment will affect the culture and fermentation of the culture medium; the current storage device can generally only perform simple placement and storage, with a single function and low practicality. For example, when supplementing additives to the culture medium, repeated storage and retrieval are required, which easily increases the risk of contamination, and operations such as shaking the culture tube need to be performed manually, making the storage operation more cumbersome and complicated; based on this, the present invention proposes a storage device with rich functions, enhanced storage convenience, and capable of supplementing additives, shaking the culture tube, and reducing the difficulty of operation. Summary of the invention

[0003] In view of the above technical problems, the present invention is rich in functions, enhances the convenience of storage, can supplement additives, achieves shaking of culture tubes, and reduces the difficulty of operation.

[0004] The scheme used in the present invention is: a constant temperature storage device for polysialic acid fermentation medium, including a storage container, a take-and-place hole is provided on the top of the storage container, and a control system is provided on the top of the storage container, an internal array of the storage container is provided with a mounting table, and an array of placement holes is opened on the mounting table, and a positioning mechanism is provided in the placement hole; the positioning mechanism includes a mounting ring arranged in the placement hole, a ball part is connected to the mounting ring by a ball pair, and a culture tube is detachably arranged in the ball part; a support tube is connected to the bottom of the ball part, and the support tube is deflected to shake the culture tube; a transfer mechanism is provided inside the storage container for transferring and taking out the culture tube; the transfer mechanism The structure includes a sliding seat slidably arranged inside the storage container, and the sliding seat is provided with a clamping mechanism for clamping the culture tube; an adding mechanism is arranged at the bottom end of the storage container, and is used to inject additives into the culture tube; the adding mechanism includes a supplementary tube slidably fitted at the bottom end of the storage container, and the supplementary tube is clamped and transferred by the clamping mechanism on the sliding seat; a blocking mechanism is arranged on the side surface of the storage container, and when the culture tube is shaken, the blocking mechanism blocks the open end of the culture tube; the blocking mechanism includes a support plate, and sealing tubes are arranged in an array on the support plate, and the sealing tube is connected to the support plate by a telescopic rope, and a clamping ring is also arranged on the support plate to fix the sealing tube.

[0005] Furthermore, an electric cylinder is provided at the taking and placing hole, and a sealing plate is provided on the telescopic rod of the electric cylinder to block the taking and placing hole.

[0006] Furthermore, the transfer mechanism includes a transfer belt and a belt motor arranged inside the storage container, and the belt motor is used to drive the transfer belt; a slide is attached to the transfer belt, and the slide includes a connecting plate 1 and a connecting plate 2, and a guide rod 1 is fixedly connected between the connecting plate 1 and the connecting plate 2, and a screw rod 1 is rotatably connected; the connecting plate 1 and the connecting plate 2 are respectively attached to the transfer belt; the slide seat is slidably fitted on the screw rod 1 and the guide rod 1, and the slide seat and the screw rod 1 form a spiral pair, and a motor 1 is arranged on the connecting plate 2, and the motor 1 is used to drive the screw rod 1.

[0007] Furthermore, the clamping mechanism includes an electric cylinder three arranged on a sliding seat, a mounting seat one is arranged on the telescopic rod of the electric cylinder three, an electric cylinder four is arranged on the mounting seat one, a mounting seat two is arranged on the telescopic rod of the electric cylinder four, a clamping electric cylinder is arranged on the mounting seat two, and clamping parts are arranged on the telescopic rods at both ends of the clamping electric cylinder for clamping the culture tube.

[0008] Furthermore, the adding mechanism includes an additive preparation box arranged in an array on the storage container, the replenishing tube and the additive preparation box are connected by a transmission tube, and the additive is transmitted through the additive preparation box; a limiting wheel is arranged at the bottom end of the storage container, which is used to limit the transmission tube between the replenishing tube and the additive preparation box; a replenishing release part is arranged on the replenishing tube, which is used to release the additive; a clearing mechanism is arranged at the bottom end of the storage container, which is used to clear the adding mechanism; and an installation cylinder is arranged at the bottom end of the storage container, and a slide groove is opened on the installation cylinder, and the replenishing tube slides in the slide groove of the installation cylinder.

[0009] Furthermore, the cleaning mechanism includes a cleaning collection box arranged on the storage container, and a cleaning docking box arranged at the bottom end of the storage container, the cleaning docking box is located below the replenishing release part, and the cleaning docking box is connected to the cleaning collection box; a docking hole is arranged on the cleaning docking box, and an electric cylinder 2 is arranged inside the storage container, the electric cylinder 2 is used to control the movement of the cleaning docking box, and the docking hole on the cleaning docking box cooperates with the release port of the replenishing release part to achieve communication.

[0010] Furthermore, a driving motor is arranged on the mounting platform, and a driving shaft is arranged on the output shaft of the driving motor, and the driving shaft is used to drive the support cylinder on the positioning mechanism to deflect; the positioning mechanism includes a groove ring arranged at the bottom of the mounting platform, and an arc groove is opened in the central area of ​​the groove ring; a swivel is rotatably mounted on the groove ring, a radial guide rod is arranged on the inner side of the swivel, and a connecting driving wheel is arranged on one end of the radial guide rod extending to the central area of ​​the swivel, and the connecting driving wheels on each positioning mechanism are connected through a belt mechanism, one end of the belt mechanism is connected to the driving shaft, and the belt mechanism is driven by the driving motor.

[0011] Furthermore, a radial seat is slidably installed on the radial guide rod, and a spring 2 for providing elastic force is sleeved on the radial guide rod, a contact wheel is arranged on the electric cylinder 1, the contact wheel is located in the arc groove, and moves along the inner side of the arc groove, a connecting rod is rotatably installed on the radial seat, and one end of the connecting rod is rotatably connected to the support tube; an electromagnetic clamping rod is arranged on the support tube for fixing the bottom of the culture tube; a connecting rod is arranged on the support tube, and the top of the connecting rod is connected to the ball part; a spherical positioning rod is telescopically arranged on the inner side of the ball part, and a spring 1 for providing elastic force is connected between the spherical positioning rod and the ball part, and the spherical positioning rod is used to position the culture tube.

[0012] Furthermore, the sealing mechanism includes a control electric cylinder 1 arranged on the storage container, and a control frame arranged on the inner side of the storage container, the control frame is driven by the control electric cylinder 1; a support plate is installed on the control frame, a control electric cylinder 2 is arranged on the support plate, a mounting bracket is arranged on the telescopic rod of the control electric cylinder 2, and a clamping ring is installed on the mounting bracket; an electromagnetic clamping rod 2 is arranged on the clamping ring for fixing the sealing tube; an electromagnetic clamping rod 3 is arranged on the sealing tube for clamping the open end of the culture tube.

[0013] Compared with the prior art, the present invention has the following advantages: (1) During the culture process, when the culture medium is supplemented with additives, the additives can be transferred through the additive preparation box, that is, the operator prepares the types of additives to be supplemented in the additive preparation box, transfers them through the additive preparation box, and finally passes through the supplementation tube and is released from the supplementation release part; (2) The supplementation tube is transferred to the location of the target culture tube, and the additive is released through the supplementation release part to complete the supplementation of the additive. Furthermore, the position is changed in turn to supplement the additives in each culture medium, instead of manual addition, and repeated removal of the culture medium is avoided to reduce pollution; (3) The addition mechanism is cleared, that is, the electric cylinder is used to clean the additive mechanism. Second, control the movement of the cleaning docking box so that the docking hole on the cleaning docking box cooperates with the replenishing release part, replenishes clean water in the additive preparation box, and transmits it to clean the transmission path, and the clean water is finally collected by the clean collection box; (4) The contact wheel moves on the inner side of the arc groove to achieve radial position change, that is, the radial seat changes position. Under the connection of the connecting rod, the support tube shakes, that is, the culture tube shakes to achieve even shaking; (5) When taking and placing the culture tube, the position can be adjusted through the transfer mechanism. At the target culture tube, the clamping part is controlled by the clamping electric cylinder to clamp the culture tube. The installation seat one is controlled by the electric cylinder three to move, that is, the culture tube is pulled out of the positioning mechanism to complete the transfer. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0015] Figure 2 It is a schematic diagram of the internal structure of the storage container of the present invention.

[0016] Figure 3 This is a schematic diagram of the bottom installation structure inside the storage container of the present invention.

[0017] Figure 4 It is a schematic diagram of the transfer mechanism structure of the present invention.

[0018] Figure 5 It is a schematic diagram of the installation structure of the installation platform of the present invention.

[0019] Figure 6 This is a schematic diagram of the installation structure of the positioning mechanism of the present invention.

[0020] Figure 7 It is a schematic diagram of the support tube installation structure of the present invention.

[0021] Figure 8 It is a schematic diagram of the groove ring structure of the present invention.

[0022] Fig. 9 It is a schematic diagram of the swivel installation structure of the present invention.

[0023] Fig.10It is a schematic diagram of the radial seat installation structure of the present invention.

[0024] Fig.11 It is a schematic diagram of the structure of the blocking mechanism of the present invention.

[0025] Fig.12 It is a schematic diagram of the support plate installation structure of the present invention.

[0026] Fig.13 It is a schematic diagram of the sealing cylinder installation structure of the present invention.

[0027] Figure numerals: 1-storage container; 2-additive preparation box; 3-control system; 4-electric cylinder 1; 5-sealing plate 1; 6-transfer belt; 7-belt motor; 8-mounting table; 9-culture tube; 10-cleaning collection box; 11-clearing docking box; 12-electric cylinder 2; 13-limiting wheel; 14-mounting cylinder; 15-supplementing tube; 16-supplementing release part; 17-motor 1; 18-screw rod 1; 19-guide rod 1; 20-connecting plate 1; 21-connecting plate 2; 22-sliding seat; 23-electric cylinder 3; 24-mounting seat 1; 25-electric cylinder 4; 26-mounting seat 2; 27-clamping electric cylinder; 28-clamping part; 29-driving Motor; 30-driving shaft; 31-mounting ring; 32-ball; 34-spherical positioning rod; 3401-spring one; 35-support cylinder; 3501-electromagnetic clamp rod one; 36-groove ring; 3601-arc groove; 37-connecting rod; 38-connecting rod; 39-swivel; 40-radial seat; 41-spring two; 42-radial guide rod; 43-connecting drive wheel; 44-contact wheel; 45-control electric cylinder one; 46-control frame; 47-support plate; 48-control electric cylinder two; 49-mounting bracket; 50-clamping ring; 5001-electromagnetic clamp rod two; 51-sealing cylinder; 5101-electromagnetic clamp rod three; 52-telescopic rope. DETAILED DESCRIPTION

[0028] Example: Figures 1 to 13As shown, a pick-up and release hole is provided on the top of the storage container 1, and a control system is provided on the top of the storage container 1 for adjusting the storage temperature and performing pick-up and release control. An electric cylinder 4 is provided at the pick-up and release hole, and a sealing plate 5 is provided on the telescopic rod of the electric cylinder 4 for blocking the pick-up and release hole; a mounting table 8 is provided in the internal array of the storage container 1, and a placement hole is provided in the array on the mounting table 8, and a positioning mechanism is provided in the placement hole; the positioning mechanism includes a mounting ring 31 provided in the placement hole, and a ball portion 32 is connected to the mounting ring 31 by a ball pair, and the ball portion 32 A culture tube 9 is detachably arranged in the middle; a support tube 35 is connected to the bottom of the ball part 32, and the support tube 35 is deflected to shake the culture tube 9; a driving motor 29 is arranged on the mounting platform 8, and a driving shaft 30 is arranged on the output shaft of the driving motor 29, and the driving shaft 30 is used to drive the support tube 35 on the positioning mechanism to deflect; the positioning mechanism includes a groove ring 36 arranged at the bottom of the mounting platform 8, and an arc groove 3601 is opened in the central area of ​​the groove ring 36; a rotating ring 39 is rotatably installed on the groove ring 36, and a radial guide rod is arranged on the inner side of the rotating ring 39 42, a connecting drive wheel 43 is arranged on one end of the radial guide rod 42 extending to the central area of ​​the rotating ring 39, and the connecting drive wheels 43 on each positioning mechanism are connected through a belt mechanism, one end of the belt mechanism is connected to the driving shaft 30, and the belt mechanism is driven by the driving motor 29; a radial seat 40 is slidably mounted on the radial guide rod 42, and a spring 2 41 for providing elastic force is sleeved on the radial guide rod 42, and a contact wheel 44 is arranged on the electric cylinder 1 4, and the contact wheel 44 is located in the arc groove 3601 and moves along the arc groove 3601 moves inside, a connecting rod 38 is rotatably installed on the radial seat 40, one end of the connecting rod 38 is rotatably connected to the support tube 35; an electromagnetic clamping rod 3501 is provided on the support tube 35 for fixing the bottom of the culture tube 9; a connecting rod 37 is provided on the support tube 35, and the top of the connecting rod 37 is connected to the ball part 32; a spherical positioning rod 34 is telescopically provided on the inside of the ball part 32, a spring 3401 for providing elastic force is connected between the spherical positioning rod 34 and the ball part 32, and the spherical positioning rod 34 is used to position the culture tube 9.

[0029] A transfer mechanism is arranged inside the storage container 1 for transferring and placing the culture tube 9; the transfer mechanism comprises a slide seat 22 slidably arranged inside the storage container 1, and a clamping mechanism is arranged on the slide seat 22 for clamping the culture tube 9; the transfer mechanism comprises a transfer belt 6 and a belt motor 7 arranged inside the storage container 1, and the belt motor 7 is used to drive the transfer belt 6; a slide is attached to the transfer belt 6, and the slide comprises a connecting plate 1 20 and a connecting plate 2 21, and a guide rod 19 is fixedly connected between the connecting plate 1 20 and the connecting plate 2 21, and a screw rod 18 is rotatably connected; the connecting plate 1 20 and the connecting plate 2 21 are respectively Attached to the transfer belt 6; the slide 22 is slidably fitted on the screw 18 and the guide rod 19, and the slide 22 and the screw 18 form a spiral pair, and a motor 17 is arranged on the connecting plate 21, and the motor 17 is used to drive the screw 18; the clamping mechanism includes an electric cylinder 3 23 arranged on the slide 22, a mounting seat 1 24 is arranged on the telescopic rod of the electric cylinder 3 23, an electric cylinder 4 25 is arranged on the mounting seat 1 24, a mounting seat 26 is arranged on the telescopic rod of the electric cylinder 4 25, a clamping electric cylinder 27 is arranged on the mounting seat 26, and clamping parts 28 are arranged on the telescopic rods at both ends of the clamping electric cylinder 27 for clamping the culture tube 9.

[0030] An adding mechanism is provided at the bottom of the storage container 1 for injecting additives into the culture tube 9; the adding mechanism includes a supplementing tube 15 that is slidably fitted at the bottom of the storage container 1, and the supplementing tube 15 is clamped and transferred by a clamping mechanism on the slide 22; a blocking mechanism is provided on the side of the storage container 1, and when the culture tube 9 is shaken, the blocking mechanism blocks the open end of the culture tube 9; the blocking mechanism includes a support plate 47, and an array of sealing tubes 51 are provided on the support plate 47, and the sealing tubes 51 are connected to the support plate 47 by a telescopic rope 52, and a clamping ring 50 is also provided on the support plate 47 for fixing the sealing tube 51; the adding mechanism includes an additive preparation box 2 arranged in an array on the storage container 1, the replenishing pipe 15 is connected to the additive preparation box 2 through a transmission pipe, and the additive is transmitted through the additive preparation box 2; a limiting wheel 13 is arranged at the bottom end of the storage container 1, which is used to limit the transmission pipe between the replenishing pipe 15 and the additive preparation box 2; a replenishing release part 16 is arranged on the replenishing pipe 15, which is used to release the additive; a cleaning mechanism is arranged at the bottom end of the storage container 1, which is used to clean the adding mechanism; a mounting cylinder 14 is arranged at the bottom end of the storage container 1, and a mounting cylinder 14 is opened There is a slide groove, and the replenishing pipe 15 is slidably matched in the slide groove of the mounting cylinder 14; the cleaning mechanism includes a cleaning collection box 10 arranged on the storage container 1, and a cleaning docking box 11 arranged at the bottom end of the storage container 1, the cleaning docking box 11 is located below the replenishing release part 16, and the cleaning docking box 11 is connected to the cleaning collection box 10; a docking hole is arranged on the cleaning docking box 11, and an electric cylinder 12 is arranged inside the storage container 1, and the electric cylinder 12 is used to control the movement of the cleaning docking box 11, and the docking hole on the cleaning docking box 11 cooperates with the release port of the replenishing release part 16 to realize the connection. The sealing mechanism comprises a control electric cylinder 1 45 arranged on the storage container 1, and a control frame 46 arranged on the inner side of the storage container 1, the control frame 46 is driven by the control electric cylinder 1 45; a support plate 47 is installed on the control frame 46, a control electric cylinder 2 48 is arranged on the support plate 47, a mounting bracket 49 is arranged on the telescopic rod of the control electric cylinder 2 48, and a clamping ring 50 is installed on the mounting bracket 49; an electromagnetic clamping rod 2 5001 is arranged on the clamping ring 50 for fixing the sealing tube 51; an electromagnetic clamping rod 3 5101 is arranged on the sealing tube 51 for clamping the open end of the culture tube 9.

[0031] The working principle is as follows: the prepared culture medium is placed in a storage container 1 for storage, that is, fermented and cultured in a culture tube 9; a sealing plate 5 is used to block the top access hole of the storage container 1 to achieve airtightness; during the storage process, the temperature can be controlled by a control system 3 to achieve a constant internal temperature; during the culture process, when the culture medium is supplemented with additives, the additives can be transferred through the additive preparation box 2, that is, the operator prepares the types of additives that need to be supplemented in the additive preparation box 2, transfers them through the additive preparation box 2, and finally passes through the supplementation tube 15 and is released from the supplementation release part 16. Specifically, the supplementation release part 16 needs to be aligned with the corresponding culture tube 9. Open end; that is, through the operation of the belt motor 7, the transfer belt 6 moves, so that the connecting plate 20 and the connecting plate 21 move to adjust the height; the motor 17 works, the screw rod 18 rotates, and the slide 22 moves to adjust the lateral position. At the target position, the clamping part 28 is controlled by the clamping electric cylinder 27 to clamp the supplementary tube 15 to transfer the supplementary tube 15, and the supplementary tube 15 is separated from the mounting tube 14; the supplementary tube 15 is transferred to the location of the target culture tube 9, and is released through the supplementary release part 16 to complete the supplementation of additives. Further, the position is changed in turn to supplement the additives in each culture medium, instead of manual addition, and repeated removal of the culture medium is avoided to reduce pollution.

[0032] Furthermore, the adding mechanism can be cleaned, that is, the electric cylinder 12 controls the movement of the cleaning docking box 11 so that the docking hole on the cleaning docking box 11 cooperates with the replenishing release part 16, replenishes clean water in the additive preparation box 2, and transmits it to clean the transmission path, and the clean water is finally collected by the clean collection box 10.

[0033] After adding the additive, the culture tube 9 is shaken to evenly mix the additive. Specifically, the control frame 46 is moved by controlling the electric cylinder 1 45 to move the seal 51 above the culture tube 9. The installation bracket 49 is moved by controlling the electric cylinder 2 48, that is, the installation bracket 49 drives the clamping ring 50 to move. The clamping ring 50 transfers the seal 51 to fit the open end of the culture tube 9. The electromagnetic clamping rod 3 5101 is clamped and fixed at the open end of the culture tube 9, and the electromagnetic clamping rod 2 5001 releases the seal 51, so that the seal 51 is sleeved on the culture tube 9, which can prevent the culture medium from overflowing when shaking. Furthermore, The driving motor 29 works, the driving shaft 30 rotates, and under the belt drive, the connected driving wheel 43 rotates, that is, the swivel 39 rotates, the radial seat 40 rotates with the swivel 39, and the contact wheel 44 moves inside the arc groove 3601 to achieve radial position change, that is, the radial seat 40 changes position, and under the connection of the connecting rod 38, the support tube 35 shakes, that is, the culture tube 9 shakes to achieve even shaking; the bottom end of the culture tube 9 is located on the support tube 35 and is clamped by an electromagnetic clamp rod 3501. The culture tube 9 passes through the ball part 32 and is limited by the spherical positioning rod 34. The spherical positioning rod 34 is telescopically changed according to the size of the culture tube 9.

[0034] When taking and placing the culture tube 9, the position can be adjusted through the above-mentioned transfer mechanism. At the target culture tube 9, the electric cylinder four 25 controls the telescopic position of the mounting seat two 26, and the clamping electric cylinder 27 controls the clamping part 28 to clamp the culture tube 9. The electric cylinder three 23 controls the movement of the mounting seat one 24, that is, the culture tube 9 is pulled out of the positioning mechanism to complete the transfer.

Claims

1. A constant temperature storage device for polysialic acid fermentation medium, comprising a storage container (1), wherein a take-in and put-out hole is provided on the top of the storage container (1), and a control system is provided on the top of the storage container (1), characterized in that: The storage container (1) is provided with a mounting table (8) in an array, and placement holes are provided in an array on the mounting table (8), and a positioning mechanism is provided in the placement hole; the positioning mechanism comprises a mounting ring (31) arranged in the placement hole, and a ball portion (32) is connected to the mounting ring (31) by a ball pair, and a culture tube (9) is detachably arranged in the ball portion (32); a support tube (35) is connected to the bottom of the ball portion (32), and the support tube (35) is deflected to shake the culture tube (9); a transfer mechanism is provided inside the storage container (1) for transferring and placing the culture tube (9); the transfer mechanism comprises a slide seat (22) slidably arranged inside the storage container (1), and a clamping mechanism is provided on the slide seat (22) for clamping the culture tube (9); an adding mechanism is arranged at the bottom end of the storage container (1) for injecting additives into the culture tube (9); the adding mechanism comprises a supplementing tube (15) slidably fitted at the bottom end of the storage container (1), the supplementing tube (15) being clamped and transferred by a clamping mechanism on a slide seat (22); a blocking mechanism is arranged on the side of the storage container (1), and when the culture tube (9) is shaken, the blocking mechanism blocks the open end of the culture tube (9); the blocking mechanism comprises a support plate (47), a sealing tube (51) is arranged in an array on the support plate (47), the sealing tube (51) and the support plate (47) are connected by a telescopic rope (52), and a clamping ring (50) is also arranged on the support plate (47) for fixing the sealing tube (51); The adding mechanism comprises an additive preparation box (2) arranged in an array on the storage container (1); the replenishing pipe (15) is connected to the additive preparation box (2) through a transmission pipe, and the additive is transmitted through the additive preparation box (2); a limiting wheel (13) is arranged at the bottom end of the storage container (1) to limit the transmission pipe between the replenishing pipe (15) and the additive preparation box (2); a replenishing release part (16) is arranged on the replenishing pipe (15) to release the additive; a cleaning mechanism is arranged at the bottom end of the storage container (1) to clean the adding mechanism; a mounting tube (14) is arranged at the bottom end of the storage container (1), and a slide groove is provided on the mounting tube (14), and the replenishing pipe (15) is slidably fitted in the slide groove of the mounting tube (14); The cleaning mechanism comprises a cleaning collection box (10) arranged on the storage container (1), and a cleaning docking box (11) arranged at the bottom end of the storage container (1), the cleaning docking box (11) being located below the supplementary release portion (16), and the cleaning docking box (11) being connected to the cleaning collection box (10); a docking hole is arranged on the cleaning docking box (11), and a second electric cylinder (12) is arranged inside the storage container (1), the second electric cylinder (12) being used to control the movement of the cleaning docking box (11), and the docking hole on the cleaning docking box (11) cooperates with a release port of the supplementary release portion (16) to achieve communication.

2. A constant temperature storage device for polysialic acid fermentation medium according to claim 1, characterized in that: An electric cylinder (4) is arranged at the taking-in and putting-out hole, and a sealing plate (5) is arranged on the telescopic rod of the electric cylinder (4) for sealing the taking-in and putting-out hole.

3. A constant temperature storage device for polysialic acid fermentation medium according to claim 1, characterized in that: The transfer mechanism comprises a transfer belt (6) and a belt motor (7) arranged inside the storage container (1), the belt motor (7) being used to drive the transfer belt (6); a slide is attached to the transfer belt (6), the slide comprises a connecting plate 1 (20) and a connecting plate 2 (21), a guide rod 1 (19) is fixedly connected between the connecting plate 1 (20) and the connecting plate 2 (21), and a screw rod 1 (18) is rotatably connected thereto; the connecting plate 1 (20) and the connecting plate 2 (21) are respectively attached to the transfer belt (6); a slide seat (22) is slidably fitted on the screw rod 1 (18) and the guide rod 1 (19), and the slide seat (22) and the screw rod 1 (18) form a spiral pair; a motor 1 (17) is arranged on the connecting plate 2 (21), and the motor 1 (17) is used to drive the screw rod 1 (18).

4. A constant temperature storage device for polysialic acid fermentation medium according to claim 1, characterized in that: The clamping mechanism comprises an electric cylinder three (23) arranged on a sliding seat (22), a mounting seat one (24) being arranged on a telescopic rod of the electric cylinder three (23), an electric cylinder four (25) being arranged on the mounting seat one (24), a mounting seat two (26) being arranged on the telescopic rod of the electric cylinder four (25), a clamping electric cylinder (27) being arranged on the mounting seat two (26), and clamping parts (28) being arranged on the telescopic rods at both ends of the clamping electric cylinder (27) for clamping the culture tube (9).

5. A constant temperature storage device for polysialic acid fermentation medium according to claim 1, characterized in that: The mounting platform (8) is provided with a driving motor (29), and a driving shaft (30) is provided on the output shaft of the driving motor (29), and the driving shaft (30) is used to drive the support cylinder (35) on the positioning mechanism to deflect; the positioning mechanism comprises a groove ring (36) arranged at the bottom of the mounting platform (8), and an arc groove (3601) is opened in the central area of ​​the groove ring (36); a rotating ring (39) is rotatably mounted on the groove ring (36), and a radial guide rod (42) is provided on the inner side of the rotating ring (39); a connecting driving wheel (43) is provided on one end of the radial guide rod (42) extending to the central area of ​​the rotating ring (39), and the connecting driving wheels (43) on the positioning mechanisms are connected via a belt mechanism, and one end of the belt mechanism is connected to the driving shaft (30), and the belt mechanism is driven by the driving motor (29).

6. A constant temperature storage device for polysialic acid fermentation medium according to claim 5, characterized in that: A radial seat (40) is slidably mounted on the radial guide rod (42), and a spring (41) for providing elastic force is sleeved on the radial guide rod (42). A contact wheel (44) is arranged on the electric cylinder (4), and the contact wheel (44) is located in the arc groove (3601) and moves along the inner side of the arc groove (3601). A connecting rod (38) is rotatably mounted on the radial seat (40), and one end of the connecting rod (38) is rotatably connected to the support tube (35); an electromagnetic clamping rod (3501) is arranged on the support tube (35) for fixing the bottom of the culture tube (9); a connecting rod (37) is arranged on the support tube (35), and the top end of the connecting rod (37) is connected to the ball part (32); a spherical positioning rod (34) is telescopically arranged on the inner side of the ball part (32), and a spring (3401) for providing elastic force is connected between the spherical positioning rod (34) and the ball part (32), and the spherical positioning rod (34) is used to position the culture tube (9).

7. A constant temperature storage device for polysialic acid fermentation medium according to claim 1, characterized in that: The blocking mechanism comprises a control electric cylinder 1 (45) arranged on the storage container (1), and a control frame (46) arranged on the inner side of the storage container (1), the control frame (46) being driven by the control electric cylinder 1 (45); a support plate (47) being mounted on the control frame (46), a control electric cylinder 2 (48) being arranged on the support plate (47), a mounting bracket (49) being arranged on the telescopic rod of the control electric cylinder 2 (48), and a clamping ring (50) being mounted on the mounting bracket (49); an electromagnetic clamping rod 2 (5001) being arranged on the clamping ring (50) for fixing the sealing tube (51); and an electromagnetic clamping rod 3 (5101) being arranged on the sealing tube (51) for clamping the open end of the culture tube (9).

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