Composition for improving sleep quality and preparation method and application thereof

Abstract

The present invention belongs to the field of food technology, and specifically relates to a composition for improving sleep quality, and a preparation method and application thereof. The raw materials of the composition include: casein hydrolyzed peptide, γ-aminobutyric acid, jujube seed powder, tea theanine, bovine spleen peptide powder, ampelopsis serrulata extract and pyrroloquinoline quinone disodium salt. The raw materials of the composition for improving sleep quality in the present invention are all derived from food. Compared with common sedative drugs, it has good safety, no dependence and tolerance, and no adverse reactions.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and in particular relates to a composition for improving sleep quality, a preparation method and an application thereof. Background Art

[0002] Sleep disorders refer to various functional disorders manifested in the sleep-wake process, including insomnia, excessive sleepiness, sleep breathing disorders and abnormal sleep behavior caused by various reasons. The main clinical manifestations are abnormal sleep quantity (excessive or insufficient) and paroxysmal abnormalities during sleep (sleepwalking, sleep talking, nightmares, night terrors, teeth grinding, involuntary muscle or limb twitching). Sleep disorders are mostly caused by long-term ideological conflicts or excessive mental burden, mental labor, long-term improper work-rest combination, physical weakness after illness, etc.

[0003] Insomnia can cause fatigue, uneasiness, general discomfort, listlessness, slow reaction, headache, and inattention. In severe cases, it can lead to functional diseases such as schizophrenia, depression, anxiety, autonomic nervous system dysfunction, and various systemic diseases (such as cardiovascular system, digestive system, etc.).

[0004] At present, Western medicine mainly uses drugs for treatment, which can be divided into six categories: ① benzodiazepines ② antidepressants ③ antihistamines ④ barbiturates and non-barbiturates ⑤ antipsychotics and other sedatives ⑥ melatonin. Among them, benzodiazepines are the main drugs, and their main pharmacological effects are anti-anxiety, sedation, hypnosis, anticonvulsant and skeletal muscle relaxation. The main action pathway is to promote the release of inhibitory neurotransmitters such as γ-aminobutyric acid (GABA) and glycine by inhibitory neurons in the brain, inhibit the production and release of 5-HT, and thus produce an anti-anxiety effect. However, long-term and large-scale use of commonly used benzodiazepines and non-benzodiazepine sedatives and hypnotic drugs will produce serious adverse reactions, making patients prone to tolerance and dependence.

[0005] Traditional Chinese medicine advocates treatment through Chinese herbal prescriptions, acupuncture, and acupoint massage, but it has the disadvantages of taking a long time and having a slow effect.

[0006] Chinese invention patent application CN107594269A discloses a formula of a polypeptide herbal solid beverage with the function of improving sleep, which includes the following components by weight: 2.5-2.9 parts of bone marrow peptide powder, 0.1-0.5 parts of brain peptide powder, 1.0-5.0 parts of sea buckthorn powder, 1.0-5.0 parts of lily powder, 1.0-5.0 parts of hawthorn powder, 1.0-5.0 parts of spiny jujube seed powder, 1.0-3.0 parts of kudzu root powder, 0.5-3.0 parts of ginkgo powder, 0.5-3.0 parts of cassia seed powder, and 0.5-3.0 parts of angelica powder. However, the above formula ingredients are relatively complex.

[0007] Chinese invention patent application CN101361916A discloses a health product for enhancing human immunity and promoting sleep. The Chinese medicine preparation is an oral preparation made of Chinese angelica, Poria, velvet antler, dendrobium, ginseng, raw rehmannia root, eucommia bark, ligusticum chuanxiong, rosa laevigata, and epimedium as raw materials, and pharmaceutically permitted auxiliary materials. However, the invention mainly involves Chinese herbal extracts, which have many medicinal flavors and contain precious Chinese medicinal materials such as velvet antler, and the cost is relatively high.

[0008] Therefore, it is very necessary to develop a natural, side-effect-free, safe and mild food that can effectively improve sleep, and a drug that can prevent or treat sleep disorders to alleviate and solve the pain of patients. Summary of the invention

[0009] In view of the deficiencies in the prior art, the present invention provides a composition for improving sleep quality, and a preparation method and application thereof.

[0010] In order to achieve the above-mentioned purpose of the present invention, the specific technical solution adopted by the present invention is:

[0011] A composition for improving sleep quality, the raw materials of the composition comprising: casein hydrolyzed peptide, gamma-aminobutyric acid, jujube seed powder, tea theanine, bovine spleen peptide powder, ampelopsis serrulata extract and pyrroloquinoline quinone disodium salt.

[0012] Preferably, the raw materials of the composition include, by weight: 20-30 parts of casein hydrolyzed peptide, 10-20 parts of γ-aminobutyric acid, 5-10 parts of jujube seed powder, 1-3 parts of tea theanine, 5-10 parts of bovine spleen peptide powder, 5-10 parts of Ampelopsis grossedentata extract and 0.1-0.3 parts of pyrroloquinoline quinone disodium salt.

[0013] Further preferably, the raw materials of the composition include, by weight: 25 parts of casein hydrolyzed peptide, 15 parts of γ-aminobutyric acid, 8 parts of Ziziphus jujuba seed powder, 2 parts of tea theanine, 8 parts of bovine spleen peptide powder, 8 parts of Agave sphenanthera extract and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0014] Gamma-aminobutyric acid (GABA) is a natural non-protein amino acid and an important inhibitory neurotransmitter in the central nervous system of mammals. About 50% of central nervous synapses use GABA as a medium. When the human body lacks GABA, it will produce emotions such as anxiety, restlessness, fatigue, and worry.

[0015] Theanine exhibits a variety of pharmacological effects in the body, including prevention of cardiovascular diseases, effects on the nervous system, regulation of blood sugar, adjuvant therapy or treatment of tumors, anti-oxidation and cell protection, etc. Among them, the effect of theanine on the nervous system is mainly manifested in promoting the release of dopamine in the brain, increasing α-brain waves, reducing β-brain waves, thereby improving sleep and regulating emotions.

[0016] The seeds of the Chinese jujube tree are the dried mature seeds of the plant Chinese jujube of the Rhamnaceae family. They contain flavonoid glycosides, triterpenes, saponins, organic acids and other ingredients. Traditional Chinese medicine believes that Chinese jujube seeds have the effects of nourishing the liver, calming the heart and soothing the nerves. Modern pharmacological studies have shown that Chinese jujube seeds can effectively calm the nerves and have a significant effect on insomniacs. Chinese jujube seeds significantly improve sleep conditions mainly by regulating the functions of the internal organs and blood of the human body, and rarely produce toxic side effects.

[0017] Casein hydrolyzed peptide is the hydrolysis product of milk protein, which is obtained by trypsin hydrolysis of milk protein. It contains bioactive decapeptides, has a relaxing effect, has no toxic side effects, has a clear effect of relieving tension and improving sleep, and does not produce habitual dependence or addiction like tranquilizers.

[0018] Bovine spleen peptide usually uses pure and natural bovine spleen as raw material, and is processed through crushing, sterilization, bio-enzymatic hydrolysis, enzyme inactivation, purification, concentration, centrifugal spraying and drying to obtain small molecule bovine spleen peptide powder. Bovine spleen peptide powder has very high activity, is easily absorbed by the human body, can relieve fatigue and enhance immunity, has the effect of sobering up and protecting the liver, and can help lower blood lipids and blood pressure.

[0019] Ampelopsis grossedentata is the dried young branches and leaves of Ampelopsis grossedentata (Hand.-Mazz.) WT Wang, a plant of the Vitaceae family. It tastes sweet, light and cool. It enters the lung, liver and stomach meridians. It can clear away heat and detoxify, promote dampness and reduce swelling. It is used for colds and fever, sore throat, damp-heat jaundice, red and swollen eyes, carbuncles, sores and boils.

[0020] Pyrroloquinoline quinone disodium salt is the sodium salt of pyrroloquinoline quinone (PQQ), and its chemical name is 4,5-dihydro-4,5-dioxy-1-hydropyrrole (2,3f) quinone-2,7,9-tricarboxylic acid disodium salt. It can support the growth and development of mitochondria, stimulate the rapid growth of human cells, and has strong antioxidant properties. It can help remove free radicals and reduce cell damage. It has a powerful effect on cardiovascular health, brain health, metabolic function health, etc. It can mainly help physical recovery and anti-fatigue.

[0021] The present invention also relates to a method for preparing the above composition, comprising the following steps:

[0022] (1) Mix casein hydrolyzed peptide, jujube seed powder, bovine spleen peptide powder and Ampelopsis serrulata extract evenly, and sieve to obtain a mixture 1;

[0023] (2) Gamma-aminobutyric acid, tea theanine and pyrroloquinoline quinone disodium salt are mixed with mixture 1 and sieved to obtain the composition.

[0024] Preferably, the sieving in steps (1) and (2) is through a 50-60 mesh sieve.

[0025] The present invention also relates to the application of the composition in preparing a food product for improving sleep quality.

[0026] Preferably, the food product for improving sleep quality further comprises a food-acceptable auxiliary material.

[0027] Preferably, the food product is in the form of powder, granules, tablets or solutions.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The raw materials of the composition for improving sleep quality in the present invention are all derived from food. Compared with common sedative drugs, it has good safety, no dependence and tolerance, and no adverse reactions.

[0030] (2) The present invention has discovered the optimal combination of jujube seed powder, bovine spleen peptide powder, and Ampelopsis serrulata extract. Under the optimal combination of raw materials, the sleep duration and sleep frequency can be significantly improved, thereby better regulating the body's sleep quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a comparison chart of the sleeping efficiency before and after taking the compositions of Examples 1-3 of the present invention and Comparative Examples 1-6;

[0032] Figure 2 is a comparison chart of sleep duration maintenance rate before and after taking the compositions of Examples 1-3 of the present invention and Comparative Examples 1-6;

[0033] Figure 3 It is a comparison chart of sleep quality before and after taking the compositions of Examples 1-3 of the present invention and Comparative Examples 1-6. DETAILED DESCRIPTION

[0034] The present invention will be further described in detail below in conjunction with specific examples. The following examples are not intended to limit the present invention, but are only intended to illustrate the present invention. The experimental methods used in the following examples are generally conventional, unless otherwise specified, and the materials, reagents, etc. used in the following examples are commercially available, unless otherwise specified.

[0035] Casein hydrolyzed peptides were purchased from Zhejiang Nuo Derivative Technology Co., Ltd.; Ziziphus jujuba seed powder was purchased from Shaanxi Tianxingjian Biochemical Technology Co., Ltd.; tea theanine was purchased from Xicheng International Trade (Shanghai) Co., Ltd.; bovine spleen peptide powder was purchased from Dezhou Lanli Biotechnology Co., Ltd.; Agave sphenanthera extract (specification 10%) was purchased from Xi'an Nuozhong Kangjian Biotechnology Co., Ltd.; pyrroloquinoline quinone disodium salt was purchased from Shandong Yiran Health Technology Group Co., Ltd.

[0036] Example 1

[0037] A composition for improving sleep quality comprises the following raw materials by weight: 25 parts of casein hydrolyzed peptide, 15 parts of gamma-aminobutyric acid, 8 parts of jujube seed powder, 2 parts of tea theanine, 8 parts of bovine spleen peptide powder, 8 parts of Ampelopsis grossedentata extract and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0038] The preparation method is:

[0039] (1) Mix casein hydrolyzed peptide, jujube seed powder, bovine spleen peptide powder and Ampelopsis serrulata extract evenly, and pass through a 60-mesh sieve to obtain a mixture 1;

[0040] (2) Gamma-aminobutyric acid, tea theanine and pyrroloquinoline quinone disodium salt are mixed with mixture 1 and passed through a 60-mesh sieve to obtain the composition.

[0041] Example 2

[0042] A composition for improving sleep quality comprises the following raw materials by weight: 20 parts of casein hydrolyzed peptide, 10 parts of gamma-aminobutyric acid, 5 parts of jujube seed powder, 1 part of tea theanine, 5 parts of bovine spleen peptide powder, 5 parts of Ampelopsis grossedentata extract and 0.1 part of pyrroloquinoline quinone disodium salt.

[0043] The preparation method is:

[0044] (1) Mix casein hydrolyzed peptide, jujube seed powder, bovine spleen peptide powder and Ampelopsis serrulata extract evenly, and pass through a 50-mesh sieve to obtain a mixture 1;

[0045] (2) Gamma-aminobutyric acid, tea theanine and pyrroloquinoline quinone disodium salt are mixed with mixture 1 and passed through a 50-mesh sieve to obtain the composition.

[0046] Example 3

[0047] A composition for improving sleep quality comprises the following raw materials by weight: 30 parts of casein hydrolyzed peptide, 20 parts of gamma-aminobutyric acid, 10 parts of jujube seed powder, 3 parts of tea theanine, 10 parts of bovine spleen peptide powder, 10 parts of Ampelopsis grossedentata extract and 0.3 parts of pyrroloquinoline quinone disodium salt.

[0048] The preparation method is consistent with that in Example 1.

[0049] Comparative Example 1

[0050] The difference between this comparative example and Example 1 is that the amount of the powder of Ziziphus jujuba seed is 24 parts, the amount of the powder of bovine spleen peptide is 0 parts, and the amount of the extract of Ampelopsis grossedentata is 0 parts.

[0051] Comparative Example 2

[0052] The difference between this comparative example and Example 1 is that the content of the powder of Ziziphus jujuba seed is 0 parts, the powder of bovine spleen peptide is 12 parts, and the extract of Ampelopsis grossedentata is 12 parts.

[0053] Comparative Example 3

[0054] The difference between this comparative example and Example 1 is that 8 parts of Ziziphus jujuba seed powder, 16 parts of bovine spleen peptide powder, and 0 parts of Ampelopsis serrulata extract are used.

[0055] Comparative Example 4

[0056] The only difference between this comparative example and Example 1 is that 8 parts of Ziziphus jujuba seed powder, 0 parts of bovine spleen peptide powder, and 16 parts of Ampelopsis grossedentata extract are used.

[0057] Comparative Example 5

[0058] The difference between this comparative example and Example 1 is only that the raw material ratio is different.

[0059] The raw materials of the composition are as follows: 25 parts of casein hydrolyzed peptide, 30 parts of gamma-aminobutyric acid, 15 parts of jujube seed powder, 1 part of tea theanine, 3 parts of bovine spleen peptide powder, 4 parts of ampelopsis serrulata extract and 0.5 parts of pyrroloquinoline quinone disodium salt by weight.

[0060] Comparative Example 6

[0061] The only difference between this comparative example and Example 1 is that pyrroloquinoline quinone disodium salt is not added.

[0062] Effect test

[0063] Test Example 1

[0064] 100 mice of similar size were selected and divided into 10 groups, each with 10 mice, and the compositions prepared in Examples 1-3 and Comparative Examples 1-6 were gavaged at a dose of 100 mg / kg. A blank control group was gavaged with normal saline, and then the sleep latency (the time when the righting reflex disappears) and sleep duration (the time from the disappearance of the righting reflex to the recovery) of the mice in each group were observed and recorded. The results are shown in Table 1.

[0065] Table 1 Sleep onset latency and sleep duration

[0066]

[0067] Note: Different letters in the same column indicate significant statistical differences between the corresponding groups, p < 0.05.

[0068] According to Table 1, the mice taking the compositions of Examples 1-3 significantly shortened their sleep latency and significantly prolonged their sleep duration. The compositions prepared in Examples 1-3 of the present invention have the effect of treating insomnia.

[0069] Test Example 2

[0070] 100 subjects were recruited. The subjects were required to be healthy and without obvious physical defects. They were divided into 10 groups, with 10 subjects in each group. A blank control group was set up to observe the sleep quality before taking the composition of the present invention. The combination of Examples 1-3 and Comparative Examples 1-6 took the compositions prepared by Examples 1-3 and Comparative Examples 1-6 respectively. The sleep status of the subjects was observed by a wristband that detects sleep status. The sleep efficiency before and after taking the composition of the present invention was as follows: Figure 1 As shown in Figure 2, the sleep duration maintenance rate is as follows: Figure 2 As shown in Figure 3 shown.

[0071] Conclusion: After taking the compositions of Examples 1-3 of the present invention and Comparative Examples 1-6, the efficiency of falling asleep is improved, the sleep maintenance rate is improved, and the proportion of deep sleep is high. By comparing Comparative Examples 1-6 with Example 1, it is found that there is a synergistic effect between the Ampelopsis serrulata extract, bovine spleen peptide powder and Ziziphus jujuba seed powder. After taking Examples 1-3, the effect is better than that of Comparative Examples 1-6, and the effect of Example 1 is more significant.

[0072] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A composition for improving sleep quality, It is characterized in that The raw materials of the composition include, by weight: 20-30 parts of casein hydrolyzed peptide, 10-20 parts of gamma-aminobutyric acid, 5-10 parts of jujube seed powder, 1-3 parts of tea theanine, 5-10 parts of bovine spleen peptide powder, 5-10 parts of ampelopsis serrulata extract and 0.1-0.3 parts of pyrroloquinoline quinone disodium salt.

2. The composition according to claim 1, It is characterized in that The raw materials of the composition include, by weight: 25 parts of casein hydrolyzed peptide, 15 parts of gamma-aminobutyric acid, 8 parts of jujube seed powder, 2 parts of tea theanine, 8 parts of bovine spleen peptide powder, 8 parts of ampelopsis serrulata extract and 0.2 parts of pyrroloquinoline quinone disodium salt.

3. A method for preparing the composition according to any one of claims 1 to 2, It is characterized in that The following steps are involved: (1) Mix casein hydrolyzed peptide, jujube seed powder, bovine spleen peptide powder and Ampelopsis serrulata extract evenly, and sieve to obtain a mixture 1; (2) Gamma-aminobutyric acid, tea theanine and pyrroloquinoline quinone disodium salt are mixed with mixture 1 and sieved to obtain the composition.

4. The preparation method according to claim 3, It is characterized in that In steps (1) and (2), the sieving is performed through a 50-60 mesh sieve.

5. Use of the composition according to any one of claims 1 to 2 in preparing a food product for improving sleep quality.

6. The use according to claim 5, It is characterized in that The food product for improving sleep quality also includes a food-acceptable auxiliary material.

7. The use according to claim 6, It is characterized in that The food product is in the form of powder, granules, tablets or solutions.

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