Aegerita acutula and its application in promoting the growth of Dendrobium officinale

Abstract

The present invention discloses an Aschersonia sp. strain Gaoshan-15 and its application in promoting the growth of Dendrobium officinale, belonging to the field of microbial technology. The strain Gaoshan-15 was isolated from wild-collected Dendrobium officinale and has a preservation number of CGMCC NO. 18547. When the Aschersonia sp. strain Gaoshan-15 provided by the present invention symbionts with Dendrobium officinale, it can significantly promote the growth of Dendrobium officinale. The seedling height growth rate is 57.73% higher than that of the control plants, and the fresh weight growth rate is 34.5% higher than that of the control plants. The strain Gaoshan-15 provides a new option for the industrial cultivation of Dendrobium officinale.

Description

Technical Field

[0001] The present invention relates to Aschersonia sp., in particular to Aschersonia sp. strain Gaoshan-15 that can promote the growth of Dendrobium officinale, belonging to the field of microbial technology. Background Art

[0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium in Orchidaceae. It has functions such as promoting fluid production, reducing blood sugar, enhancing the body's immunity, and resisting skin aging, and has high application value in food, medicine, and health care. Due to the limited number of wild Dendrobium officinale, it is listed as a third-level protected variety in the "Regulations on the Protection and Management of Wild Medicinal Resources"; with the aggravation of the reduction of wild Dendrobium officinale resources, it is included in the "Red Data Book of Chinese Plants" as an endangered plant. Currently, the Dendrobium officinale supplied in the market mainly comes from artificial cultivation. However, the growth rate of artificially cultivated Dendrobium officinale is slow and the average yield is low.

[0003] The mycorrhizal fungi symbiotic with Dendrobium officinale can improve its germination rate, survival rate, and promote growth and development by helping the roots absorb nutrients and water. Isolating and screening mycorrhizal fungi beneficial to its growth from wild Dendrobium officinale can be used for the industrial cultivation of Dendrobium officinale to improve its growth rate. Reasonably utilizing mycorrhizal resources and improving the disease resistance of Dendrobium officinale can reduce the application amount of chemical pesticides to a certain extent, which also conforms to the sustainable development strategy of agriculture. Therefore, the research on mycorrhizal inoculants has become an important direction in the Dendrobium officinale industry. Summary of the Invention

[0004] Object of the Invention: The object of the present invention is to provide an Aschersonia sp. strain Gaoshan-15 that can promote the growth of Dendrobium officinale, and to provide its application in improving the growth rate of Dendrobium officinale.

[0005] Technical Solution: The present invention provides an Aschersonia sp. strain Gaoshan-15, which is classified and named as Aschersonia of the genus Aschersonia in Deuteromycetes, with the Latin scientific name of Aschersonia sp., deposited in the China General Microbiological Culture Collection Center (CGMCC), with the deposit number of CGMCC NO.18547 and the deposit date of September 18, 2019.

[0006] In June 2013, the strain Gaoshan-15 was obtained by isolation and purification from the roots of wild Dendrobium collected in Longling County, Dehong Prefecture, Yunnan Province. This strain has a significant growth-promoting effect on Dendrobium officinale and is identified as Aschersonia sp.

[0007] The colony morphological characteristics of Aschersonia sp. Gaoshan-15 are as follows: villous mycelium, slightly with grey pigment; small conidia are unicellular, ovoid, borne on solitary phialides, and often aggregated into clusters at the top of phialides.

[0008] The present invention provides a product comprising the strain Gaoshan-15. The product includes solid bacterial agents and / or bacterial solutions.

[0009] The present invention provides the application of the strain Gaoshan-15 or the product in promoting the growth of Dendrobium officinale.

[0010] Preferably, the specific method of the application is to add the strain Gaoshan-15 described in claim 1 or the product described in claim 2 to the cultivation substrate of Dendrobium officinale.

[0011] Preferably, the added mass is 1-2 g per plant of Dendrobium officinale.

[0012] Preferably, the relative water content of the substrate is not less than 60%. Co-cultivate for about 10 days to ensure the survival of the mycelium in the substrate.

[0013] Preferably, the adding method is to place it on the surface of the substrate.

[0014] Preferably, the adding method is to place it at the root of Dendrobium officinale.

[0015] Preferably, the application method is to co-cultivate the strain Gaoshan-15 described in claim 1 or the product described in claim 2 with Dendrobium officinale.

[0016] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages: 1. When the Aschersonia strain Gaoshan-15 provided by the present invention coexists with Dendrobium officinale, it can significantly promote the growth of Dendrobium officinale. The seedling height growth rate is 57.73% higher than that of the control plants, and the fresh weight growth rate is 34.5% higher than that of the control plants; 2. The Aschersonia strain Gaoshan-15 provided by the present invention has simple culture conditions, is easy to preserve, is easy for industrial production, and has good development and application prospects. Description of the Drawings

[0017] Figure 1 It is the colony characteristic diagram of the strain Gaoshan-15 (1a is the front; 1b is the back);

[0018] Figure 2 It is the morphological comparison diagram of Dendrobium officinale inoculated and not inoculated with the strain Gaoshan-15 (inoculated on the right of the figure; not inoculated on the left of the figure).

[0019]

Preservation Information

[0020] The technical solution of the present invention will be further described below with reference to the accompanying drawings.

[0021] Example 1 Morphological Characteristics of Strain Gaoshan-15

[0022] The strain Aschersonia sp. Gaoshan-15 was cultured on a PDA plate, and its morphological characteristics were observed. The colony morphology was observed with the naked eye. As Figure 1 shown, the mycelium of the strain Aschersonia sp. Gaoshan-15 was villous and slightly with grey pigment. The small conidia were unicellular, ovoid, borne on solitary phialides, and often aggregated into clusters at the top of the phialides.

[0023] Example 2 Taxonomic Identification of Strain Gaoshan-15

[0024] The genomic DNA of the strain Aschersonia sp. Gaoshan-15 was extracted, and the full ITS region sequence of the strain Aschersonia sp. Gaoshan-15 was amplified with universal primers for the fungal ITS region and sequenced. The full sequence obtained by PCR amplification was as shown in SEQ ID No: 1. After comparison and identification, it was confirmed that the strain Aschersonia sp. Gaoshan-15 belonged to the fungus Aschersonia sp. of the Deuteromycetes class.

[0025] Example 3 Establishment of the Symbiotic System between Strain Gaoshan-15 and Dendrobium officinale

[0026] Prepare the solid inoculum of the strain Aschersonia sp. Gaoshan-15: Using cottonseed hull fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and load it into a high-pressure sterilization bag. Sterilize it for 20 minutes under the high-pressure conditions of 121 °C and 101.3 kPa. After the culture substrate cooled, inoculate the strain Aschersonia sp. Gaoshan-15 under sterile conditions and conduct artificial culture. The specific culture conditions were 26 °C, 12 h light + 12 h darkness, pH 6.5, and culture for 25 days. After the culture was completed, process the inoculum into a solid inoculum in the shape of 1 cm3 blocks.

[0027] Establish the symbiotic system between the strain Aschersonia sp. Gaoshan-15 and Dendrobium officinale: Place the solid inoculum blocks on the surface of the cultivation substrate for Dendrobium officinale seedlings. The inoculation amount of the solid inoculum for each cluster (5 plants) of Dendrobium officinale seedlings was 5 g. After applying the inoculum, the relative water content of the substrate was maintained above 60% for 10 days.

[0028] Example 4 Strain Gaoshan-15 promotes the growth of Dendrobium officinale

[0029] This example uses the solid bacterial agent of strain Gaoshan-15 prepared in Example 3.

[0030] Set up the inoculated Gaoshan-15 plants: Select the Dendrobium officinale plants with basically the same growth during inoculation, and measure the seedling height and ground diameter. Use 15 cm diameter nutrient pots for cultivating Dendrobium officinale, and cultivate one clump of Dendrobium officinale in each nutrient pot. Place the block solid inoculant on the surface of the cultivation substrate of the Dendrobium officinale seedlings planted that year. The inoculation amount of solid inoculant for each clump (5 plants) of Dendrobium officinale seedlings is 5 g. After applying the inoculant, the relative moisture content of the substrate is maintained above 60% for 10 days.

[0031] The control plants (CK) were set up: Dendrobium officinale plants that were not inoculated with mycorrhizal agents but only inoculated with an equal amount of cottonseed hull residue. Other cultivation conditions were the same as those of the inoculated Gaoshan-15 plants.

[0032] Detection of the growth of Dendrobium officinale: Six months after inoculation, the mycorrhizal degree of the root system of Dendrobium officinale was detected by section staining to ensure that the inoculated strain Gaoshan-15 had combined with the root of Dendrobium officinale and formed mycorrhiza. Twelve months after inoculation, the growth promotion effect of mycorrhized plants of Dendrobium officinale was analyzed, and the detection indicators included seedling height and fresh weight. The test results are shown in Table 1, and the morphology of Dendrobium officinale inoculated with and without the inoculation strain Gaoshan-15 is shown in Figure 2 .

[0033] Table 1 The growth-promoting effect of Gaoshan-15 on Dendrobium officinale plants

[0034] Plant Seedling height (cm) before inoculation Seedling height (cm) after inoculation Fresh weight (g) before inoculation Fresh weight (g) after inoculation Growth rate of seedling height Growth rate of fresh weight Inoculated with Alpine - 15 8.6±0.2 17.1±0.3 8.8±0.15 15.80±0.20 98.84% 79.55% CK 9±0.2 12.7±0.1 9.1±0.15 13.20±0.21 41.11% 45.05%

Claims

1. A conchosporon ( Aschersonia sp. ) strain Gaoshan-15, characterized in that The deposit number of the strain Gaoshan-15 is CGMCC NO.18547.

2. A product, characterized in that: The product contains the strain Gaoshan-15 described in claim 1.

3. The product according to claim 2, characterized in that The product is a solid bacterial agent or a bacterial liquid.

4. Use of the strain Gaoshan-15 according to claim 1 or the product according to claim 2 in promoting the growth of Dendrobium officinale.

5. The use according to claim 4, characterized in that: The specific method of the application is to add the strain Gaoshan-15 described in claim 1 or the product described in claim 2 to the cultivation medium of Dendrobium officinale.

6. The use according to claim 5, characterized in that: The added mass is 1-2 g per Dendrobium officinale plant.

7. The use according to claim 5, characterized in that: The relative water content of the matrix is ​​not less than 60%.

8. The use according to claim 5, characterized in that: The adding method is to place it on the surface of the matrix.

9. The use according to claim 5, characterized in that: The adding method is to place the plant at the root of Dendrobium officinale.

10. The use according to claim 4, characterized in that: The application method is to symbiotically cultivate the strain Gaoshan-15 described in claim 1 or the product described in claim 2 with Dendrobium officinale.

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