A transgenic plant identification device and method using fluorescent protein as reporter gene

Abstract

The present invention relates to a transgenic plant identification device and method using fluorescent protein as a reporter gene. The device comprises a shell, a translation and lifting mechanism is arranged at the bottom of the shell, a light reflection mechanism is fixedly connected to the middle of two opposite inner side walls of the shell, a camera is detachably connected to the middle of the top of the shell, the camera end of the camera is located inside the shell, a fixing part is fixedly connected to the top wall of the shell, the fixing part is adapted to the camera end of the camera, an inner box and a plurality of illuminators are also fixedly connected to the top wall of the shell, light holes adapted to the camera end of the camera are opened in the middle of the top and the middle of the bottom of the inner box, a light adjustment mechanism is arranged inside the inner box, the light adjustment mechanism is adapted to the camera end of the camera, a transparent glass is fixedly connected to the middle of one side wall of the shell, and a light filtering protection mechanism is correspondingly arranged on the side of the transparent glass close to the inside of the shell. The present invention can effectively detect and screen the whole and tissues of transgenic plants using fluorescent protein as a reporter gene.

Description

Technical Field

[0001] The invention belongs to the technical field of plant molecular biology, and in particular relates to a transgenic plant identification device and method using fluorescent protein as a reporter gene. Background Art

[0002] With the continuous development of biotechnology, transgenic technology has become an important means in the field of modern agriculture. Transgenic technology can be used to introduce target genes with excellent traits into the plant genome, thereby improving the genetic traits of the plant. However, during the transformation process, only a few plant cells can absorb foreign DNA and integrate it into the plant genome, and most cells are not transformed. Therefore, the introduction of target genes often requires the use of screening marker genes to give transformed cells specific selective markers in order to identify and identify transgenic plants.

[0003] Fluorescent proteins can excite bright green fluorescence at specific wavelengths, are non-toxic to cells, have stable fluorescence, and do not require substrates. They have become an indispensable tool in the field of biological and medical research. As a reporter gene, fluorescent proteins can be used to visually distinguish transgenic plants by observing the luminescence of fluorescent proteins. They are highly sensitive, highly repeatable, and applicable to different species, playing an important role in screening and detecting transgenic plants. Based on the green fluorescent protein gene and the red fluorescent protein gene, a large number of fluorescent protein gene mutants have been developed, and their fluorescent colors cover almost all visible light spectra. The new proteins produced by the transformation of the green fluorescent protein in the original Aequorea victoria jellyfish have colors ranging from blue to yellow and have been widely used in life science research. The study of mutants of the red fluorescent protein DsRED and the optimization of a series of monomers have obtained long-wavelength fluorescent proteins with various colors of emission light in the orange and red spectral regions. The monomeric proteins with better performance include mCherry, mOrange, etc. In addition, many natural fluorescent proteins of various colors have been found in coral polyps and their related species, such as bright red, orange, gray, brown, tan, yellow, etc., which greatly enrich the spectral diversity of fluorescent proteins and provide more options for life science research. Summary of the invention

[0004] The purpose of the present invention is to provide a transgenic plant identification device and method using fluorescent protein as a reporter gene, so as to detect and screen the entire transgenic plant and tissues using fluorescent protein as a reporter gene.

[0005] To achieve the above-mentioned purpose, the present invention provides the following scheme: a transgenic plant screening and detection device using fluorescent protein as a reporter gene, comprising a shell, a translation and lifting mechanism is arranged at the bottom end of the shell, a light reflecting mechanism is fixedly connected to the middle of two opposite inner side walls of the shell, a camera is detachably connected to the middle of the top end of the shell, the camera end of the camera is located inside the shell, a fixing part is fixedly connected to the top wall of the shell, the fixing part is adapted to the camera end of the camera, an inner box and a plurality of lighting lamps are also fixedly connected to the top wall of the shell, light holes adapted to the camera end of the camera are opened in the middle of the top end and the middle of the bottom end of the inner box, A light adjustment mechanism is arranged inside the inner box body, and the light adjustment mechanism is adapted to the camera end of the camera, a plurality of the lighting lamps are located outside the fixing portion, a transparent glass is fixedly connected to the middle of one side wall of the outer shell, a light filtering and protection mechanism is arranged correspondingly on the side of the transparent glass close to the inside of the outer shell, a sealing door is arranged on the side wall of the outer shell adjacent to the transparent glass, a control panel is fixedly connected to the top end of the outer shell, a plurality of buttons are arranged on the control panel, and the translation and lifting mechanism, the light reflecting mechanism, the fixing portion, the light adjustment mechanism, the light filtering and protection mechanism, the camera, the lighting lamps and the control panel are electrically connected.

[0006] Preferably, the translation and lifting mechanism includes a first motor, the first motor is fixedly connected to the inner bottom wall of the shell, the first motor output shaft is fixedly connected to one end of a horizontally arranged first screw, the other end of the first screw is rotatably connected to the inner wall of the shell, the middle of the first screw is threadedly connected to a movable plate, the movable plate and the first screw are perpendicular to each other and the bottom end is in sliding contact with the bottom wall of the shell, one end of the top of the movable plate is fixedly connected to the second motor, the second motor output shaft is fixedly connected to one end of a horizontally arranged second screw, the second screw is arranged along the length direction of the movable plate, the other end of the second screw is rotatably connected to a second fixed seat, the bottom end of the second fixed seat is fixedly connected to the top end of the movable plate, the middle of the second screw is threadedly connected to a moving block, the bottom end of the moving block is in sliding contact with the top end of the movable plate, the top of the moving block is fixedly connected to a lifting part, the first motor, the second motor, the lifting part and the control board are electrically connected.

[0007] Preferably, the lifting part includes four first electrically-controlled telescopic rods, which are respectively arranged at the four corners of the top of the moving block, the first electrically-controlled telescopic rods are vertically arranged and the bottom ends are fixedly connected to the top of the moving block, and the tops of the four first electrically-controlled telescopic rods are commonly fixedly connected with a placement plate, the top of the placement plate is used to place the plant to be detected, and the first electrically-controlled telescopic rods are electrically connected to the control board.

[0008] Preferably, the light reflecting mechanism includes a reflective strip and a fixing plate, the reflective strip is fixedly connected to an inner side wall of the outer shell, the fixing plate is fixedly connected to the other inner side wall of the outer shell, the reflective strip is located below the fixing plate, the bottom end of the fixing plate is rotatably connected to a light reflecting part, the reflective strip is inclined and its reflective surface faces the light reflecting part, and the light reflecting part is electrically connected to the control panel.

[0009] Preferably, the light reflecting part includes a rotating frame, a reflector is fixedly connected to the inside of the rotating frame, the reflective surface of the reflector faces the reflective strip, one end of the top of the rotating frame is fixedly connected to a bottom hinge seat, the bottom hinge seat is rotatably connected to one end of a second electrically-controlled telescopic rod, the other end of the second electrically-controlled telescopic rod is rotatably connected to a top hinge seat, the top hinge seat is fixedly connected to the bottom end of the fixed plate, one end of a rotating shaft is respectively fixedly connected to the middle of two opposite outer side walls of the rotating frame, the other end of the rotating shaft is rotatably connected to the bottom end of the fixed rod, the top end of the fixed rod is fixedly connected to the bottom end of the fixed plate, and the second electrically-controlled telescopic rod is electrically connected to the control panel.

[0010] Preferably, the light adjustment mechanism includes an image magnifying unit and a multi-color light filtering unit, and the image magnifying unit and the multi-color light filtering unit are symmetrically arranged on both sides of the camera end, and the image magnifying unit and the multi-color light filtering unit are fixedly connected to the inner wall of the inner box body, and the image magnifying unit and the multi-color light filtering unit are electrically connected to the control board.

[0011] Preferably, the image magnifying unit comprises a fifth motor, the fifth motor is fixedly connected to the top wall of the inner box body, the fifth motor output shaft is fixedly connected to the top of a fifth rotating shaft arranged vertically, the bottom of the fifth rotating shaft is rotatably connected to the bottom wall of the inner box body, a central connecting rod is fixedly connected to the middle of the outer wall of the fifth rotating shaft, both ends of the central connecting rod are respectively fixedly connected to first fixing rings, a convex lens is fixedly connected inside the first fixing ring, the two convex lenses have different refractive indices for light, and the fifth motor is electrically connected to the control board;

[0012] The multi-color light filtering unit includes a sixth motor, which is fixedly connected to the top wall of the inner box body, the sixth motor output shaft is fixedly connected to the top of a vertically arranged sixth rotating shaft, the bottom end of the sixth rotating shaft is rotatably connected to the bottom wall of the inner box body, the outer side wall of the sixth rotating shaft is fixedly connected to a ring, the outer side wall of the ring is fixedly connected to one end of a plurality of connecting blocks arranged at equal intervals, the other end of the connecting block is fixedly connected to a second fixing ring, the second fixing ring is located below the first fixing ring, the second filter plate is fixedly connected inside the second fixing ring, and the sixth motor is electrically connected to the control board.

[0013] Preferably, the light filtering protection mechanism includes a fixed frame, which is fixedly connected to the inner wall of the outer shell and corresponds to the transparent glass, two vertical inner walls of the fixed frame are respectively provided with two sliding grooves, a first filter plate is slidably arranged in the sliding groove, a fourth screw is threadedly passed through one end of the first filter plate, the fourth screw is vertically arranged and both ends are rotatably connected to the two horizontal side walls of the fixed frame, the bottom end of the fourth screw is fixedly connected to the output shaft of the fourth motor, the fourth motor is fixedly connected to the outer bottom end of the fixed frame, a second guide rod is vertically arranged slidably passed through the other end of the first filter plate, both ends of the second guide rod are fixedly connected to the two horizontal side walls of the fixed frame, the two fourth motors are respectively located at the two ends of the bottom of the fixed frame, and the fourth motor is electrically connected to the control board.

[0014] Preferably, the fixing part includes a third motor, the third motor is fixedly connected to the inner top wall of the shell, the output shaft of the third motor is fixedly connected to one end of a horizontally arranged two-way screw, the other end of the two-way screw is rotatably connected to a third fixing seat, the top end of the third fixing seat is fixedly connected to the inner top wall of the shell, the two ends of the two-way screw are respectively threadedly connected to one end of a moving beam, the two moving beams are horizontally spaced and perpendicular to the two-way screw, the other ends of the two moving beams are slidably connected to the same first guide rod, the first guide rod and the two-way screw are parallel to each other, the two ends of the first guide rod are respectively fixedly connected to the first fixing seat, the top end of the first fixing seat is fixedly connected to the inner top wall of the shell, the middle parts of the side walls of the two moving beams close to each other are respectively fixedly connected with clamping blocks, the two clamping blocks are adapted to the camera end of the camera, and the third motor is electrically connected to the control board.

[0015] A transgenic plant identification device and method using fluorescent protein as a reporter gene, comprising the following steps:

[0016] S1. Install the camera on the top of the housing;

[0017] S2, open the sealed door to place the plant to be tested on the top of the translation and lifting mechanism, and adjust the horizontal position and height of the plant to be tested by the translation and lifting mechanism;

[0018] S3, turn on the lighting lamp, and adjust the light reflecting mechanism so that the light of the lighting lamp and the light reflected by the light reflecting mechanism are irradiated onto the plant to be detected;

[0019] S4. Adjust the light adjustment mechanism so that the camera can capture light of different colors emitted by the fluorescent protein.

[0020] Compared with the prior art, the present invention has the following advantages and technical effects:

[0021] The present invention can be used to carry the transgenic plants that need to be detected and screened and adjust the horizontal position and height position of the transgenic plants through the provided translation and lifting mechanism. The provided fixing part can stably fix the camera on the top of the shell to make the pictures taken by the camera clearer. The provided lighting lamp and light reflecting mechanism can irradiate more lighting light onto the transgenic plants. The provided light adjustment mechanism can adjust and filter light of different colors and magnify the transgenic plants to different multiples according to the needs of taking pictures. The provided light filtering protection mechanism can protect the transparent glass and protect the safety of the experimenters. The provided control panel can realize the automatic operation of the whole device. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative labor:

[0023] Figure 1 This is a schematic diagram of the appearance of the present invention;

[0024] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0025] Figure 3 The structure of the light filtering protection mechanism of the present invention is shown in FIG. Figure 1 ;

[0026] Figure 4 The structure of the light filtering protection mechanism of the present invention is shown in FIG. Figure 2 ;

[0027] Figure 5 This is a schematic diagram of the structure of the light reflection mechanism of the present invention;

[0028] Figure 6 It is a schematic diagram of the structure of the fixing part of the present invention;

[0029] Figure 7 It is a structural schematic diagram of the light adjustment mechanism of the present invention.

[0030] Among them, 1. shell; 2. transparent glass; 3. sealed door; 4. handle; 5. universal wheel; 6. control panel; 7. button; 8. camera; 9. first motor; 10. first screw; 11. moving plate; 12. second motor; 13. second screw; 14. second fixed seat; 15. moving block; 16. first electric telescopic rod; 17. placement plate; 18. reflective strip; 19. fixed plate; 20. rotating frame; 21. fixed frame; 22. first filter plate; 23. lighting lamp; 24. third motor; 25. bidirectional screw; 26. third fixed seat; 27. first guide rod; 28. , the first fixed seat; 29, the moving beam; 30, the inner box; 31, the slide; 32, the second guide rod; 33, the fourth screw; 34, the fourth motor; 35, the reflector; 36, the fixed rod; 37, the rotating shaft; 38, the second electric telescopic rod; 39, the top hinge seat; 40, the bottom hinge seat; 41, the connecting rod; 42, the clamping block; 43, the fifth motor; 44, the fifth rotating shaft; 45, the central connecting rod; 46, the first fixed ring; 47, the convex lens; 48, the sixth motor; 49, the sixth rotating shaft; 50, the collar; 51, the connecting block; 52, the second fixed ring; 53, the second filter plate. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] Reference Figures 1 to 7As shown, the present invention provides a transgenic plant identification device using fluorescent protein as a reporter gene, comprising a shell 1, a translation and lifting mechanism is arranged at the bottom end of the shell 1, a light reflecting mechanism is fixedly connected to the middle of two opposite inner side walls of the shell 1, a camera 8 is detachably connected to the middle of the top of the shell 1, the camera end of the camera 8 is located inside the shell 1, a fixing part is fixedly connected to the top wall of the shell 1, the fixing part is adapted to the camera end of the camera 8, an inner box 30 and a plurality of illuminating lamps 23 are also fixedly connected to the top wall of the shell 1, light holes adapted to the camera end of the camera 8 are opened in the middle of the top and the middle of the bottom of the inner box 30, and the inner A light adjustment mechanism is provided inside the box body 30, and the light adjustment mechanism is adapted to the camera end of the camera 8. A plurality of lighting lamps 23 are located outside the fixed portion. A transparent glass 2 is fixedly connected to the middle of one side wall of the outer shell 1, and a light filtering protection mechanism is correspondingly provided on the side of the transparent glass 2 close to the inside of the outer shell 1. A sealing door 3 is provided on the side wall of the outer shell 1 adjacent to the transparent glass 2. A control panel 6 is fixedly connected to the top end of the outer shell 1, and a plurality of buttons 7 are provided on the control panel 6. The translation and lifting mechanism, the light reflecting mechanism, the fixed portion, the light adjustment mechanism, the light filtering protection mechanism, the camera 8, the lighting lamps 23 and the control panel 6 are electrically connected.

[0034] Furthermore, a handle 4 is fixedly connected to the outer wall of the sealing door 3, and the handle 4 facilitates opening or closing the sealing door 3. Universal wheels 5 are respectively provided at the four corners of the bottom end of the shell 1, and the universal wheels 5 facilitate moving the entire device.

[0035] The shell 1 is made of an opaque material, which can make the fluorescence emitted by the transgenic plants in a dark environment clearer.

[0036] The translation and lifting mechanism provided can be used to carry the transgenic plants that need to be detected and screened, and to adjust the horizontal position and height position of the transgenic plants. The fixed portion provided can stably fix the camera 8 on the top of the housing 1, so that the pictures taken by the camera 8 are clearer. The lighting lamp 23 and the light reflecting mechanism provided can irradiate more lighting light onto the transgenic plants. The light adjusting mechanism provided can adjust and filter different colors of light and magnify the transgenic plants to different multiples according to the needs of taking pictures. The light filtering protection mechanism provided can protect the transparent glass 2 to protect the safety of the experimenters. The control panel provided can realize the automatic operation of the entire device.

[0037] There are many types of fluorescent proteins, the commonly used ones are green fluorescent protein GFP, red fluorescent protein RFP, yellow fluorescent protein YFP, blue fluorescent protein BFP, etc. Different fluorescent proteins have different excitation and emission spectra and need to be excited with excitation light of different wavelengths.

[0038] According to the characteristics of the above fluorescent proteins, the lighting lamp 23 is configured with excitation light sources of different wavelengths: 365nm, 400nm, 450nm, 510nm, 540nm. The peak value of blue light excitation light is 450nm, and various types of green fluorescent proteins can be observed. The peak value of green light excitation light is 540nm, and various types of red fluorescent proteins can be observed. The excitation light with a wavelength of 400nm can observe blue fluorescent protein. When observing green fluorescent protein, use excitation light with a wavelength of 450nm to irradiate, use the matching first filter plate 22 to protect the transparent glass 2, and then observe fluorescence. When observing red fluorescent protein, use excitation light with a wavelength of 540nm to irradiate, use the matching first filter plate 22 to protect the transparent glass 2, and then observe fluorescence. When observing blue fluorescent protein, use excitation light with a wavelength of 400nm to irradiate, and use the matching first filter plate 22 to protect the transparent glass 2.

[0039] A further optimized solution is that the translation and lifting mechanism includes a first motor 9, which is fixedly connected to the inner bottom wall of the shell 1, and the output shaft of the first motor 9 is fixedly connected to one end of a horizontally arranged first lead screw 10, and the other end of the first lead screw 10 is rotatably connected to the inner wall of the shell 1. A movable plate 11 is threadedly connected to the middle part of the first lead screw 10, and the movable plate 11 and the first lead screw 10 are perpendicular to each other and the bottom end is in sliding contact with the bottom wall of the shell 1, and a second motor 12 is fixedly connected to the top end of the movable plate 11, and the output shaft of the second motor 12 is fixedly connected to one end of a horizontally arranged second lead screw 13, and the second lead screw 13 is arranged along the length direction of the movable plate 11, and the other end of the second lead screw 13 is rotatably connected to a second fixed seat 14, and the bottom end of the second fixed seat 14 is fixedly connected to the top end of the movable plate 11, and a movable block 15 is threadedly connected to the middle part of the second lead screw 13, and the bottom end of the movable block 15 is in sliding contact with the top end of the movable plate 11, and the top of the movable block 15 is fixedly connected to a lifting part, and the first motor 9, the second motor 12, the lifting part and the control board 6 are electrically connected.

[0040] A further optimized solution is that the lifting part includes four first electrically-controlled telescopic rods 16, and the four first electrically-controlled telescopic rods 16 are respectively arranged at the four corners of the top of the moving block 15. The first electrically-controlled telescopic rods 16 are vertically arranged and the bottom ends are fixedly connected to the top of the moving block 15. The tops of the four first electrically-controlled telescopic rods 16 are commonly fixedly connected with a placement plate 17. The top of the placement plate 17 is used to place the plants to be detected, and the first electrically-controlled telescopic rods 16 are electrically connected to the control board 6.

[0041] The moving direction of the movable plate 11 and the moving direction of the movable block 15 are perpendicular to each other. The cooperation between the two can realize the movement of the transgenic plant to any position in the horizontal plane. The extension or contraction of the first electrically-controlled telescopic rod 16 can adjust the transgenic plant to any height in the vertical direction. By adjusting the position of the transgenic plant, the picture taken by the camera 8 can be clearer and more comprehensive.

[0042] A further optimized solution is that the light reflecting mechanism includes a reflective strip 18 and a fixed plate 19, the reflective strip 18 is fixedly connected to an inner side wall of the outer shell 1, the fixed plate 19 is fixedly connected to the other inner side wall of the outer shell 1, the reflective strip 18 is located below the fixed plate 19, and the bottom end of the fixed plate 19 is rotatably connected to a light reflecting part, the reflective strip 18 is tilted and its reflective surface faces the light reflecting part, and the light reflecting part is electrically connected to the control board 6.

[0043] A further optimized solution is that the light reflecting part includes a rotating frame 20, a reflector 35 is fixedly connected inside the rotating frame 20, and the reflective surface of the reflector 35 faces the reflective strip 18. A bottom hinge seat 40 is fixedly connected to one end of the top of the rotating frame 20, and the bottom hinge seat 40 is rotatably connected to one end of a second electrically-controlled telescopic rod 38, and the other end of the second electrically-controlled telescopic rod 38 is rotatably connected to a top hinge seat 39, and the top hinge seat 39 is fixedly connected to the bottom end of the fixed plate 19, and one end of a rotating shaft 37 is respectively fixedly connected to the middle of two opposite outer side walls of the rotating frame 20, and the other end of the rotating shaft 37 is rotatably connected to the bottom end of the fixed rod 36, and the top end of the fixed rod 36 is fixedly connected to the bottom end of the fixed plate 19, and the second electrically-controlled telescopic rod 38 is electrically connected to the control board 6.

[0044] The illumination light emitted by the illumination lamp 23 is reflected by the reflective strip 18 to the reflector 35, and then the inclination angle of the reflector 35 is adjusted by the extension and retraction of the second electrically controlled telescopic rod 38, which can ensure that more illumination light is concentrated on the genetically modified plants, thereby improving the accuracy of detection and screening.

[0045] A further optimized solution is that the light adjustment mechanism includes an image magnifying unit and a multi-color light filtering unit, which are symmetrically arranged on both sides of the camera end of the camera 8, and are fixedly connected to the inner wall of the inner box 30, and are electrically connected to the control board 6.

[0046] Furthermore, the top end of the inner box body 30 is fixedly connected to the inner top wall of the outer shell 1 through two connecting rods 41, and the two connecting rods 41 are located between the fixing parts.

[0047] Further optimized solution, the image magnifying part includes a fifth motor 43, the fifth motor 43 is fixedly connected to the inner top wall of the inner box body 30, the output shaft of the fifth motor 43 is fixedly connected to the top of a fifth rotating shaft 44 arranged vertically, the bottom end of the fifth rotating shaft 44 is rotatably connected to the bottom wall of the inner box body 30, the middle part of the outer wall of the fifth rotating shaft 44 is fixedly connected to a central connecting rod 45, the two ends of the central connecting rod 45 are respectively fixedly connected to a first fixing ring 46, a convex lens 47 is fixedly connected inside the first fixing ring 46, the two convex lenses 47 have different refractive indices for light, and the fifth motor 43 is electrically connected to the control board 6;

[0048] The multi-color light filtering unit includes a sixth motor 48, which is fixedly connected to the inner top wall of the inner box body 30, and the output shaft of the sixth motor 48 is fixedly connected to the top of a vertically arranged sixth rotating shaft 49, and the bottom end of the sixth rotating shaft 49 is rotatably connected to the bottom wall of the inner box body 30, and the outer side wall of the sixth rotating shaft 49 is fixedly connected to a ring 50, and the outer side wall of the ring 50 is fixedly connected to one end of a plurality of equally spaced connecting blocks 51, and the other end of the connecting block 51 is fixedly connected to a second fixing ring 52, and the second fixing ring 52 is located below the first fixing ring 46, and the second filter plate 53 is fixedly connected inside the second fixing ring 52, and the sixth motor 48 is electrically connected to the control board 6.

[0049] The two convex lenses 47 have different magnifications, and the filter colors of several second filter plates 53 are different. According to the needs of taking pictures, the convex lens 47 that meets the magnification requirements is rotated to the bottom of the camera 8, and the second filter plate 53 that meets the light color requirements is rotated to the bottom of the camera 8. Then you can start taking pictures of the genetically modified plants. After taking pictures, the other convex lenses 47 and second filter plates 53 are rotated to the bottom of the camera 8 according to different magnifications and different light colors to continue taking pictures.

[0050] A further optimized solution is provided, in which the light filtering protection mechanism includes a fixed frame 21, which is fixedly connected to the inner wall of the outer shell 1 and corresponds to the transparent glass 2. Two vertical inner walls of the fixed frame 21 are respectively provided with two slide grooves 31, in which a first filter plate 22 is slidably arranged, a fourth lead screw 33 is threadedly penetrated at one end of the first filter plate 22, the fourth lead screw 33 is vertically arranged and both ends are rotatably connected to the two horizontal side walls of the fixed frame 21, an output shaft of a fourth motor 34 is fixedly connected to the bottom end of the fourth lead screw 33, the fourth motor 34 is fixedly connected to the outer bottom end of the fixed frame 21, a second vertically arranged second guide rod 32 is slidably penetrated at the other end of the first filter plate 22, both ends of the second guide rod 32 are fixedly connected to the two horizontal side walls of the fixed frame 21, the two fourth motors 34 are respectively located at the two ends of the bottom of the fixed frame 21, and the fourth motor 34 is electrically connected to the control board 6.

[0051] During the photo-taking process, the experimenter can observe the situation inside the device through the transparent glass 2. During the observation process, the first filter plate 22 corresponding to it can be driven by different fourth motors 34 to move to the position corresponding to the transparent glass 2, thereby protecting the experimenter.

[0052] A further optimized solution is provided, wherein the fixed part comprises a third motor 24, the third motor 24 is fixedly connected to the inner top wall of the outer shell 1, the output shaft of the third motor 24 is fixedly connected to one end of a horizontally arranged bidirectional lead screw 25, the other end of the bidirectional lead screw 25 is rotatably connected to a third fixed seat 26, the top end of the third fixed seat 26 is fixedly connected to the inner top wall of the outer shell 1, the two ends of the bidirectional lead screw 25 are respectively threadedly connected to one end of a moving beam 29, the two moving beams 29 are horizontally spaced and perpendicular to the bidirectional lead screw 25, the other ends of the two moving beams 29 are slidably connected to the same first guide rod 27, the first guide rod 27 is parallel to the bidirectional lead screw 25, the two ends of the first guide rod 27 are respectively fixedly connected to the first fixed seat 28, the top end of the first fixed seat 28 is fixedly connected to the inner top wall of the outer shell 1, the middle parts of the side walls of the two moving beams 29 close to each other are respectively fixedly connected to clamping blocks 42, the two clamping blocks 42 are adapted to the camera end of the camera 8, and the third motor 24 is electrically connected to the control board 6.

[0053] After the camera 8 is fixed on the top of the housing 1 , the third motor 24 works to drive the bidirectional lead screw 25 to rotate, and the two moving beams 29 move toward each other, and finally the camera end of the camera 8 is fixed by the clamping block 42 .

[0054] A detection method for a transgenic plant screening detection device using fluorescent protein as a reporter gene comprises the following steps:

[0055] S1, installing the camera 8 on the top of the housing 1;

[0056] After the camera 8 is mounted on the top of the housing 1, the camera end of the camera 8 is further fixed by two moving beams 29 and two clamping blocks 42;

[0057] S2, open the sealed door 3 to place the plant to be tested on the top of the translation and lifting mechanism, and adjust the horizontal position and height of the plant to be tested by the translation and lifting mechanism;

[0058] Open the sealed door 3 and place the plant to be detected on the top of the placement plate 17, then adjust the position of the plant to be detected in the horizontal plane by the moving plate 11 and the moving block 15, and adjust the height of the plant to be detected in the vertical direction by the first electrically controlled telescopic rod 16;

[0059] S3, turn on the lighting lamp 23, and adjust the light reflecting mechanism so that the light of the lighting lamp 23 and the light reflected by the light reflecting mechanism are irradiated onto the plant to be detected;

[0060] The illumination light of the illumination lamp 23 will irradiate the plant to be detected, and the illumination light reflected by the reflective strip 18 and the reflector 35 will also be concentrated on the plant to be detected, thereby improving the illumination effect;

[0061] S4. Adjust the light adjustment mechanism so that the camera 8 can capture light of different colors emitted by the fluorescent protein.

[0062] According to the requirements of magnification and filtering, the corresponding convex lens 47 and the second filter plate 53 are rotated to the bottom of the camera end of the camera 8, and then the photo can be taken.

[0063] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0064] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope of the present invention.

Claims

1. A transgenic plant identification device using fluorescent protein as a reporter gene, characterized in that: The invention comprises a shell (1), wherein a translation and lifting mechanism is arranged at the bottom end of the shell (1), a light reflecting mechanism is fixedly connected to the middle parts of the two opposite inner side walls of the shell (1), a camera (8) is detachably connected to the middle part of the top end of the shell (1), the camera end of the camera (8) is located inside the shell (1), a fixing part is fixedly connected to the top wall of the shell (1), the fixing part is matched with the camera end of the camera (8), the top wall of the shell (1) is also fixedly connected to an inner box (30) and a plurality of lighting lamps (23), the middle part of the top end and the middle part of the bottom end of the inner box (30) are both provided with light holes matched with the camera end of the camera (8), a light adjusting mechanism is arranged inside the inner box (30), and the light adjusting mechanism The structure is adapted to the camera end of the camera (8), a plurality of the illuminating lamps (23) are located outside the fixing portion, a transparent glass (2) is fixedly connected to the middle of one side wall of the housing (1), a light filtering and protecting mechanism is correspondingly arranged on the side of the transparent glass (2) close to the inside of the housing (1), a sealing door (3) is arranged on the side wall of the housing (1) adjacent to the transparent glass (2), a control panel (6) is fixedly connected to the top of the outside of the housing (1), a plurality of buttons (7) are arranged on the control panel (6), and the translation and lifting mechanism, the light reflecting mechanism, the fixing portion, the light adjusting mechanism, the light filtering and protecting mechanism, the camera (8), the illuminating lamps (23) and the control panel (6) are electrically connected; The translation and lifting mechanism comprises a first motor (9), the first motor (9) being fixedly connected to the inner bottom wall of the housing (1), the output shaft of the first motor (9) being fixedly connected to one end of a first screw (10) arranged horizontally, the other end of the first screw (10) being rotatably connected to the inner wall of the housing (1), a moving plate (11) being threadedly connected to the middle of the first screw (10), the moving plate (11) and the first screw (10) being perpendicular to each other and the bottom end of the moving plate (11) being in sliding contact with the bottom wall of the housing (1), a top end of the moving plate (11) being fixedly connected to a second motor (12), the output shaft of the second motor (12) being fixedly connected to A second lead screw (13) is provided at one end thereof and is arranged along the length direction of the movable plate (11). The other end of the second lead screw (13) is rotatably connected to a second fixed seat (14). The bottom end of the second fixed seat (14) is fixedly connected to the top end of the movable plate (11). A moving block (15) is threadedly connected to the middle part of the second lead screw (13). The bottom end of the moving block (15) is in sliding contact with the top end of the movable plate (11). The top end of the moving block (15) is fixedly connected to a lifting part. The first motor (9), the second motor (12), the lifting part and the control board (6) are electrically connected. The lifting part comprises four first electrically-controlled telescopic rods (16), the four first electrically-controlled telescopic rods (16) are respectively arranged at the four corners of the top of the moving block (15), the first electrically-controlled telescopic rods (16) are arranged vertically and the bottom ends are fixedly connected to the top of the moving block (15), the top ends of the four first electrically-controlled telescopic rods (16) are commonly fixedly connected to a placement plate (17), the top end of the placement plate (17) is used to place the plant to be detected, and the first electrically-controlled telescopic rods (16) are electrically connected to the control board (6).

2. A transgenic plant identification device using fluorescent protein as a reporter gene according to claim 1, characterized in that: The light reflection mechanism comprises a reflective strip (18) and a fixing plate (19); the reflective strip (18) is fixedly connected to an inner side wall of the outer shell (1); the fixing plate (19) is fixedly connected to the other inner side wall of the outer shell (1); the reflective strip (18) is located below the fixing plate (19); a light reflection part is rotatably connected to the bottom end of the fixing plate (19); the reflective strip (18) is inclined and its reflective surface faces the light reflection part; the light reflection part is electrically connected to the control panel (6).

3. A transgenic plant identification device using fluorescent protein as a reporter gene according to claim 2, characterized in that: The light reflecting part comprises a rotating frame (20), a reflector (35) is fixedly connected inside the rotating frame (20), the reflective surface of the reflector (35) faces the reflective strip (18), one end of the top of the rotating frame (20) is fixedly connected to a bottom hinge seat (40), the bottom hinge seat (40) is rotatably connected to one end of a second electrically controlled telescopic rod (38), the other end of the second electrically controlled telescopic rod (38) is rotatably connected to a top hinge seat (39), the top hinge seat (39) is fixedly connected to the bottom end of the fixed plate (19), one end of a rotating shaft (37) is fixedly connected to the middle of two opposite outer side walls of the rotating frame (20), the other end of the rotating shaft (37) is rotatably connected to the bottom end of a fixed rod (36), the top end of the fixed rod (36) is fixedly connected to the bottom end of the fixed plate (19), and the second electrically controlled telescopic rod (38) is electrically connected to the control panel (6).

4. The transgenic plant identification device using fluorescent protein as a reporter gene according to claim 1, characterized in that: The light adjustment mechanism comprises an image magnifying unit and a multi-color light filtering unit, wherein the image magnifying unit and the multi-color light filtering unit are symmetrically arranged on both sides of the camera end of the camera (8), the image magnifying unit and the multi-color light filtering unit are fixedly connected to the inner wall of the inner box (30), and the image magnifying unit and the multi-color light filtering unit are electrically connected to the control board (6).

5. The transgenic plant identification device using fluorescent protein as a reporter gene according to claim 4, characterized in that: The image magnifying unit comprises a fifth motor (43), the fifth motor (43) is fixedly connected to the inner top wall of the inner box body (30), the output shaft of the fifth motor (43) is fixedly connected to the top end of a fifth rotating shaft (44) vertically arranged, the bottom end of the fifth rotating shaft (44) is rotatably connected to the bottom wall of the inner box body (30), a central connecting rod (45) is fixedly connected to the middle of the outer wall of the fifth rotating shaft (44), the two ends of the central connecting rod (45) are respectively fixedly connected to the first fixing ring (46), a convex lens (47) is fixedly connected inside the first fixing ring (46), the two convex lenses (47) have different refractive indices for light, and the fifth motor (43) is electrically connected to the control board (6); The multi-color light filtering unit comprises a sixth motor (48), the sixth motor (48) is fixedly connected to the inner top wall of the inner box body (30), the output shaft of the sixth motor (48) is fixedly connected to the top end of a sixth rotating shaft (49) arranged vertically, the bottom end of the sixth rotating shaft (49) is rotatably connected to the bottom wall of the inner box body (30), the outer wall of the sixth rotating shaft (49) is fixedly connected to a sleeve ring (50), the outer wall of the sleeve ring (50) is fixedly connected to one end of a plurality of connection blocks (51) arranged at equal intervals, the other end of the connection block (51) is fixedly connected to a second fixing ring (52), the second fixing ring (52) is located below the first fixing ring (46), the second fixing ring (52) is fixedly connected to a second filter plate (53), and the sixth motor (48) is electrically connected to the control board (6).

6. The transgenic plant identification device using fluorescent protein as a reporter gene according to claim 1, characterized in that: The light filtering protection mechanism comprises a fixed frame (21), the fixed frame (21) being fixedly connected to the inner side wall of the housing (1) and corresponding to the transparent glass (2), two vertical inner side walls of the fixed frame (21) respectively being provided with two slide grooves (31), a first light filter plate (22) being slidably arranged in the slide grooves (31), a fourth lead screw (33) being threadedly penetrated at one end of the first light filter plate (22), the fourth lead screw (33) being vertically arranged and having both ends being rotatably connected to the two horizontal side walls of the fixed frame (21), the first lead screw (33) being rotatably disposed at the inner side walls of the fixed frame (21), and the first lead screw (33) being rotatably disposed at the inner side walls of the fixed frame (21). The bottom end of the four lead screws (33) is fixedly connected to the output shaft of the fourth motor (34), the fourth motor (34) is fixedly connected to the outer bottom end of the fixed frame (21), the other end of the first filter plate (22) is slidably penetrated by a vertically arranged second guide rod (32), both ends of the second guide rod (32) are fixedly connected to the two horizontal side walls of the fixed frame (21), the two fourth motors (34) are respectively located at the two ends of the bottom of the fixed frame (21), and the fourth motor (34) is electrically connected to the control board (6).

7. The transgenic plant identification device using fluorescent protein as a reporter gene according to claim 1, characterized in that: The fixing portion comprises a third motor (24), the third motor (24) is fixedly connected to the inner top wall of the housing (1), the output shaft of the third motor (24) is fixedly connected to one end of a horizontally arranged bidirectional lead screw (25), the other end of the bidirectional lead screw (25) is rotatably connected to a third fixing seat (26), the top end of the third fixing seat (26) is fixedly connected to the inner top wall of the housing (1), the two ends of the bidirectional lead screw (25) are respectively threadedly connected to one end of a moving beam (29), the two moving beams (29) are horizontally spaced and perpendicular to the bidirectional lead screw (25), and the two moving beams (29) are arranged horizontally at intervals and are perpendicular to each other. The other end of the movable beam (29) is slidably connected to the same first guide rod (27), the first guide rod (27) and the bidirectional lead screw (25) are parallel to each other, the two ends of the first guide rod (27) are respectively fixedly connected to the first fixed seat (28), the top of the first fixed seat (28) is fixedly connected to the inner top wall of the shell (1), the middle parts of the side walls of the two movable beams (29) close to each other are respectively fixedly connected to the clamping blocks (42), the two clamping blocks (42) are adapted to the camera end of the camera (8), and the third motor (24) is electrically connected to the control board (6).

8. A detection method for a transgenic plant identification device using fluorescent protein as a reporter gene according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Install the camera (8) on the top of the housing (1); S2, opening the sealed door (3) to place the plant to be tested on the top of the translation and lifting mechanism, and adjusting the horizontal position and height of the plant to be tested by the translation and lifting mechanism; S3, turning on the lighting lamp (23), and adjusting the light reflecting mechanism so that the light of the lighting lamp (23) and the light reflected by the light reflecting mechanism are both irradiated onto the plant to be detected; S4. Adjust the light adjustment mechanism so that the camera (8) can capture light of different colors emitted by the fluorescent protein.

Images