Sorghum Echinococcus TL02 and Its Application

Abstract

The present invention discloses sorghum cephalosporin TL02 and its application. The present invention separates a sorghum cephalosporin (TL02) from a plant with black spot disease of long-spined thorn grass Epicoccum sorghum ) TL02, whose deposit number is CGMCC No.41277. Sorghum sorghum bacteria TL02 and the bacterial agent can control weeds such as long-spined thorn grass, and can effectively inhibit the root and bud growth of weeds such as long-spined thorn grass; at the same time, it is highly safe for pastures and crops other than corn, cabbage, soybeans, and Elymus salsa, and can safely and effectively control weeds such as long-spined thorn grass, reduce the use of chemical herbicides, and is environmentally friendly.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and particularly relates to sorghum cephalosporin TL02 and applications thereof. Background Art

[0002] Tribulus terrestris Cenchrus longispinus ) has the characteristics of high stress resistance, high reproductive capacity, and diverse transmission pathways, which will reduce the yield, quality and biodiversity of grasslands. After invading grasslands, long-spined thorn grass can reproduce rapidly, competing with other forage grasses for light, water, and fertilizer, inhibiting the growth of other forage grasses, reducing the quality of grasslands, reducing the yield of high-quality forage grasses, and endangering the ecological balance and biodiversity of grasslands.

[0003] At present, chemical control and manual mowing are commonly used to control long-spined thorn grass. These two control methods can control long-spined thorn grass in the season, but they require a lot of manpower and material resources, and the large-scale application of chemical agents increases environmental hazards and seriously affects the sustainability of agricultural ecological development. Therefore, it is very important to develop efficient and green biological herbicides. Summary of the invention

[0004] The purpose of the present invention is to provide a sorghum endococcus TL02, a bacterial agent and an application thereof.

[0005] The present invention isolated a strain TL02 with strong pathogenicity from naturally occurring long-spined Tribulus terrestris, which was identified as Echinococcus sorghum ( Epicoccum sorghinum ), has an inhibitory effect on the growth of weeds such as long-spined tribulus terrestris, and can safely and effectively prevent and control weeds such as long-spined tribulus terrestris.

[0006] The purpose of the present invention is implemented by the following technical solutions.

[0007] The present invention provides a sorghum-epiplosaccharide ( Epicoccum sorghinum )TL02, currently deposited in the China General Microbiological Culture Collection Center (CGMCC), located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Its deposit number is CGMCC No.41277, and the deposit date is April 1, 2024.

[0008] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum ) TL02 microbial agent, which includes Echinococcus kaoliang ( Epicoccum sorghinum ) TL02 and / or its fermentation broth.

[0009] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum) A method for preparing TL02 fermentation broth, comprising the following steps: 1) inoculating: Echinococcus kaoliang ( Epicoccum sorghinum 1) TL02 was inoculated on PDA solid culture medium and cultured at 25°C for 7 days to obtain activated strains; 2) Fermentation: The activated strains were inoculated into PDB liquid culture medium and cultured at 25°C and 180 rpm; 3) Filtration: The bacterial solution after 14 days of culture was centrifuged, and the supernatant obtained after centrifugation was the fermentation liquid.

[0010] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum ) TL02 or E. sorghumensis ( Epicoccum sorghinum ) Application of TL02 microbial agent in controlling long-spined tribulus terrestris.

[0011] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum ) TL02 or E. sorghumensis ( Epicoccum sorghinum ) Application of TL02 microbial agent in the preparation of products for controlling long-spined tribulus terrestris.

[0012] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum ) TL02 or E. sorghumensis ( Epicoccum sorghinum ) Application of TL02 microbial agent in inhibiting the growth of root shoots of Tribulus terrestris.

[0013] The present invention also provides a sorghum bacteria ( Epicoccum sorghinum ) TL02 or E. sorghumensis ( Epicoccum sorghinum ) Application of TL02 microbial agent in inhibiting weed growth.

[0014] Furthermore, the weeds are at least one of Amaranthus retroflexus, Chenopodium album, Echinochloa crusgalli, Chloris viridis, Setaria viridis, and Digitaria sanguinalis.

[0015] Beneficial effects: The present invention isolates sorghum spores from black spot diseased plants of long spiny tribulus terrestris ( Epicoccum sorghinum ) TL02. Sorghum Echinococcus TL02 has a strong inhibitory effect on the growth of roots, buds, stems and leaves of weeds such as long-spined thorn grass. At the same time, it is highly safe for pastures and crops other than corn, cabbage, soybeans and alkali grass. It can safely and effectively prevent weeds such as long-spined thorn grass, reduce the use of chemical herbicides, and is environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 The colony morphology of Coccus sorghumense TL02 in Example 2; the left picture is the front morphology of Coccus sorghumense TL02 colony, and the right picture is the back morphology of Coccus sorghumense TL02 colony.

[0018] Figure 2 This is the structural feature of the sorghum bacteria TL02 under a microscope in Example 2.

[0019] Figure 3 The results of spraying fermentation liquid and sterile water on Tribulus terrestris in Example 4; the left picture shows the treatment group sprayed with fermentation liquid, and the right picture shows the control group sprayed with sterile water.

[0020] Figure 4 The results of spraying fermentation liquid and sterile water on Amaranthus retroflexus in Example 5; the upper figure shows the treatment group sprayed with fermentation liquid, and the lower figure shows the control group sprayed with sterile water.

[0021] Figure 5 The results of spraying fermentation liquid and sterile water on Chenopodium album in Example 5; the upper figure shows the treatment group sprayed with fermentation liquid, and the lower figure shows the control group sprayed with sterile water.

[0022] Figure 6 The results of spraying barnyard grass with fermentation liquid and sterile water in Example 5; the left figure shows the treatment group sprayed with fermentation liquid, and the right figure shows the control group sprayed with sterile water.

[0023] Figure 7 The results of spraying fermentation liquid and sterile water on Chloris viridis in Example 5; the left figure shows the treatment group sprayed with fermentation liquid, and the right figure shows the control group sprayed with sterile water.

[0024] Figure 8 The results of spraying fermentation liquid and sterile water on Setaria viridis in Example 5; the left figure shows the treatment group sprayed with fermentation liquid, and the right figure shows the control group sprayed with sterile water.

[0025] Figure 9 The results of spraying fermentation liquid and sterile water on crabgrass in Example 5; the left figure shows the treatment group sprayed with fermentation liquid, and the right figure shows the control group sprayed with sterile water. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The formula of the culture medium used in the examples of the present invention is as follows.

[0028] PDA solid culture medium: potato 300 g / L, glucose 20 g / L, agar 15 g / L, chloramphenicol 0.1 g / L.

[0029] PDB liquid medium: potato 300 g / L, glucose 20 g / L.

[0030] MS culture medium: KNO3 1900 mg / L, NH4NO3 1650 mg / L, CaCl2 332.2 mg / L, MgSO4 180.7 mg / L, KH2PO4 170 mg / L, FeNaEDTA 36.7 mg / L, MnSO4H2O 16.9 mg / L, ZnSO4·7H2O 8.6 mg / L, H3BO3 6.2 mg / L, KI 0.83 mg / L, Na2MoO4·2H2O 0.25 mg / L, CuSO4·5H2O 0.025 mg / L, CoCl2·6H2O 0.025 mg / L.

[0031] Example 1 Isolation and screening of strain TL02.

[0032] 1. Sample collection: In October 2021, typical black spot diseased plants were collected from the long-spined Tribulus terrestris occurrence area in Dongfeng Village, Dongfeng Town, Kailu County, Tongliao City, Inner Mongolia (43.663701N, 121.579432E), stored in paper bags and numbered, and brought back to the laboratory for immediate pathogen isolation.

[0033] 2. Sample processing: Use sterile scissors to cut the diseased tissue sample (0.5 cm × 0.5 cm) at the junction of the diseased and healthy parts of the diseased plants, treat it with 30% sodium hypochlorite for 3 min, rinse it with sterile water 3 to 5 times, place it on PDA solid culture medium containing cephalosporin, and culture it in a constant temperature incubator at 25°C for 3 days.

[0034] 3. Isolation and purification of bacterial strains: After hyphae grow around the diseased tissue block, use an inoculation needle to pick up the hyphae at the edge of the colony, place it diagonally on PDA solid culture medium for purification and culture, and repeat the purification until the colony morphology is consistent; the purified colony is named and numbered TL02, and placed in a 4°C refrigerator to preserve the strain for future use.

[0035] Example 2: Identification of strain TL02.

[0036] 1. Morphological identification

[0037] The fermentation liquid of the isolated and purified TL02 strain was inoculated on PDA solid medium and cultured at a constant temperature of 25°C and a photoperiod of 12 h for 7 days. The morphology and color of the colonies were observed, the growth diameter of the colonies was measured, and the microstructural characteristics of the pathogen were observed under a 40x microscope. Figure 1 , Figure 2 shown.

[0038] 2. Molecular Biology Identification

[0039] The genomic DNA of pathogens was extracted by CTAB method and stored in a -20℃ refrigerator for future use. PCR amplification was performed using rDNA-ITS4 / 5, EF-1α, GPDH, and Actin sequence primers. The sequence primers are shown in Table 1, the PCR reaction system is shown in Table 2, and the PCR reaction program is shown in Table 3. After PCR amplification reaction, the PCR amplification product was stored at -20℃.

[0040] Table 1 PCR amplification primer sequences

[0041]

[0042] Table 2 PCR amplification reaction system

[0043]

[0044] Table 3 PCR amplification reaction program

[0045]

[0046] After the PCR amplification products were tested with 1% agarose gel, they were sent to BGI for sequencing. The nucleotide sequence length of the rDNA-ITS4 / 5 of the strain was 533 bp using ITS1 / ITS4 as primers, and the sequencing results of the ITS gene are shown in SEQ ID No.9; the nucleotide sequence length of the EF-1α of the strain was 843 bp using TEF1 / TEF2 as primers, and the sequencing results of the EF-1α gene are shown in SEQ ID No.10; the nucleotide sequence length of the GPDH of the strain was 597 bp using GPD1 / GPD2 as primers, and the sequencing results of the GPDH gene are shown in SEQ ID No.11; the nucleotide sequence length of the Actin of the strain was 1100 bp using ACTDF1 / ACTDR1 as primers, and the sequencing results of the ACTIN gene are shown in SEQ ID No.12.

[0047] The obtained sequence was compared with the known sequences in the NCBI database (http: / / www.ncbi.nlm.nih.gov / ) by BLAST and it was found that strain TL02 was similar to E. sorghumensis Epicoccum sorghinum The highest similarity was found between each amplified sequence and E. sorghumensis Epicoccum sorghinum The homology is: ITS amplification sequence and model strain Epicoccum sorghinum The homology of LC12103 is 100% (accession number MN215621.1), and the EF-1α amplified sequence is similar to the model strain Epicoccum sorghinum The homology of EL21 is 99.41% (accession number: OK236518.1), and the GPDH amplified sequence is Epicoccum sorghinum The homology of BS2-1 is 98.83% (accession number: JBCIYP010000025.1), and the Actin amplified sequence is Epicoccum sorghinum The homology of BS2-1 is 99.64% (accession number: JBCIYP010000033.1). Combined with the results of microscopic morphological identification, it can be known that the pathogen TL02 of black spot disease of Tribulus terrestris is Echinococcus sorghum Epicoccum sorghinum .

[0048] Escherichia coli ( Epicoccum sorghinum )TL02, currently deposited in the China General Microbiological Culture Collection Center (CGMCC), located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Its deposit number is CGMCC No.41277, and the deposit date is April 1, 2024.

[0049] Example 3: Sorghum spores Epicoccum sorghinum )TL02 bacterial agent.

[0050] Escherichia coli ( Epicoccum sorghinum ) TL02 bacterial agent, which includes the sorghum-attacking bacteria obtained in Example 2 ( Epicoccum sorghinum ) TL02 and / or its fermentation broth.

[0051] Escherichia coli ( Epicoccum sorghinum ) The method for preparing TL02 fermentation broth comprises the following steps:

[0052] 1) Inoculation: Inoculate the sorghum bacteria ( Epicoccum sorghinum ) TL02 was inoculated on PDA solid culture medium and cultured at 25°C for 7 days to obtain activated strains.

[0053] 2) Fermentation: Inoculate the activated strain into PDB liquid culture medium and culture in a shaker at 25°C and 180 rpm.

[0054] 3) Centrifugation: Add the bacterial liquid after 14 days of shaking culture into a sterilized 15 mL centrifuge tube and centrifuge at 10,000 rpm for 5 minutes at room temperature. The supernatant obtained after centrifugation is the fermentation liquid.

[0055] Example 4: Sorghum spores Epicoccum sorghinum ) Effects of TL02 on Tribulus terrestris.

[0056] 1. Sorghum spore bacteria ( Epicoccum sorghinum ) Effect of TL02 fermentation liquid (hereinafter referred to as fermentation liquid) on the germination of Tribulus terrestris seeds.

[0057] The seed germination rate was determined by the paper dish method. The fermentation liquid prepared in Example 3 was evenly added to the filter paper and placed in a culture dish. 25 long-spined Tribulus terrestris seeds with similar growth potential were selected and sterilized and placed in a culture dish. They were cultured in the dark at 25°C. Sterile water was set as a control group. Each treatment was repeated 3 times. The seed germination was counted after 10 days. The seed germination standard is: the germination of the buds of the seeds exceeds the length of the seeds themselves; the seed germination inhibition rate is calculated as follows: seed germination inhibition rate (%) = (control group seed germination rate - treatment group seed germination rate) / control group seed germination rate × 100%, where seed germination rate (%) = number of seeds germinating / total number of seeds tested × 100%.

[0058] The test results showed that the seed germination inhibition rate was 0.4%, indicating that the fermentation liquid had basically no inhibitory effect on the seed germination of Tribulus terrestris.

[0059] 2. The effect of fermentation liquid on the growth of root shoots of Tribulus terrestris.

[0060] A deep culture dish containing MS culture medium was prepared, and the surface of the seeds of Tribulus terrestris was disinfected and sown in the culture dish; after the seedlings of Tribulus terrestris grew for 4 days, the fermentation liquid prepared in Example 3 was added to the treatment group, and sterile water was added to the control group, and 3 replicates were set for each treatment. After 4 days of continuous cultivation, the root length inhibition rate and the shoot length inhibition rate were determined. The root length inhibition rate and the shoot length inhibition rate were calculated as follows: root length inhibition rate (%) = [(control group root length - treatment group root length) / control group root length] × 100%; shoot length inhibition rate (%) = [(control group shoot length - treatment group shoot length) / control group shoot length] × 100%.

[0061] The test results showed that the root length inhibition rate was 92.52% and the shoot length inhibition rate was 63.14%. The results showed that the fermentation liquid had an inhibitory effect on the growth of roots and shoots of Tribulus terrestris, and had a greater inhibitory effect on the growth of roots of Tribulus terrestris.

[0062] 3. The effect of fermentation liquid on the growth of stems and leaves of Tribulus terrestris.

[0063] The long-spined Tribulus terrestris was sown in a small plastic pot, and the small pot was placed in a tray filled with water, and then placed in an incubator with 18 h light and 6 h dark light to grow naturally. When the long-spined Tribulus terrestris grew to the 3-5 leaf stage, the treatment group sprayed the fermentation liquid prepared in Example 3 evenly on the leaves of the long-spined Tribulus terrestris until they were covered with dewdrops, and then covered with a black plastic bag and treated in the dark for 24 h, and the bacterial liquid was sprayed continuously for 3 days. The control group sprayed with sterile water. Each treatment was repeated 3 times, and the growth was observed every two days and the data was recorded.

[0064] The results are as follows Figure 3 As shown, the leaves of Tribulus terrestris in the treatment group turned yellow, while there was no effect in the control group, indicating that the fermentation liquid had a serious inhibitory effect on the growth of stems and leaves of Tribulus terrestris and had strong herbicidal activity.

[0065] Example 5: Sorghum spores Epicoccum sorghinum ) Pathogenicity of TL02 to common farmland weeds.

[0066] The seeds of six kinds of weeds, including Amaranthus retroflexus, Chenopodium album, Echinochloa crus-galli, Chloris viridis, Setaria viridis, and Digitaria sanguinea, were sown in small plastic pots, and the small pots were placed in a tray filled with water, and then placed in a light incubator at 25°C, 18 h light, and 6 h dark for natural growth. When the above six kinds of weeds grew to the 3-5 leaf stage, the fermentation liquid prepared in Example 3 was evenly sprayed on the stems and leaves of the weeds to be tested until they were covered with dewdrops in the treatment group, and then covered with a black plastic bag and treated in the dark for 24 h, and the bacterial liquid was sprayed continuously for 3 d. The control group was set to spray sterile water. Each treatment was repeated 3 times, and the growth was observed every two days and the data was recorded.

[0067] Crop disease susceptibility evaluation criteria: plants have no lesions, grow normally, and are asymptomatic, which is indicated by NS; plants have sporadic lesions on their leaves, and their growth and development are slightly restricted, indicating a slight impact, which is indicated by LS; 1 / 5-1 / 4 of the plant's leaf area is diseased and its growth is inhibited, which is indicated by MS; a large number of plants die and their growth and development are severely restricted, which is indicated by SS.

[0068] The results are shown in Table 4 and Figures 4 - 9 As shown, the fermentation liquid is most pathogenic to Amaranthus retroflexus, Chenopodium album, Echinochloa crusgalli and Chloris virga, and has a certain pathogenicity to Setaria viridis and Digitaria sanguinea, but not too serious.

[0069] Table 4 Pathogenicity determination of fermentation broth to common weeds

[0070]

[0071] Example 6: Sorghum Echinococcus ( Epicoccum sorghinum ) Biosafety determination of TL02.

[0072] 1. The effect of fermentation liquid on the germination of forage and crop seeds.

[0073] A total of 7 plants, including 3 kinds of forage grasses (Elymus dahliae, alfalfa, and sweet clover) and 4 kinds of crops (wheat, corn, cabbage, and oats), were selected as test subjects for the effect of fermentation liquid on the germination of forage grass and crop seeds. The seed germination rate was determined using the paper plate method. The fermentation liquid prepared in Example 3 was evenly added to the filter paper and placed in a culture dish. 25 seeds of each of the 7 kinds of forage grasses and crop seeds were selected and sterilized and placed in the culture dish respectively, and cultured at 25°C in the dark. Sterile water was set as a control group. Each treatment was repeated 3 times, and the seed germination was counted after 10 days.

[0074] The results are shown in Table 5. The fermentation liquid has an inhibitory effect on the germination of 7 kinds of forage grasses and crops. The inhibition rates of seed germination of Elymus dahliae, Sweet Clover, oats and cabbage are all above 50%. The inhibition rate of fermentation liquid on seed germination of Sweet Clover reached 91.33%. The inhibitory effect on alfalfa, wheat and corn was weaker, with inhibition rates between 10% and 30%.

[0075] 2. The effect of fermentation liquid on the growth of root shoots of forage and crops.

[0076] A total of 7 plants, including 3 kinds of forage grasses (Elymus dahliae, alfalfa, and sweet clover) and 4 kinds of crops (wheat, corn, cabbage, and oats), were selected as test subjects for the effect of fermentation liquid on the root and bud growth of forage grasses and crops. Deep culture dishes containing MS culture medium were prepared, and the surfaces of the 7 kinds of forage grasses and crop seeds were sterilized and sown in the culture dishes respectively; after the seedlings of the 7 kinds of forage grasses and crops grew for 4 days, the fermentation liquid prepared in Example 3 was added to the treatment group, and sterile water was added to the control group, and 3 replicates were set for each treatment. After continuing to culture for 4 days, the root growth inhibition rate and the shoot growth inhibition rate were determined.

[0077] The results are shown in Table 5. The fermentation liquid has an inhibitory effect on the root growth of the seven forages and crops. The inhibition rate of the root growth of Elymus dahliae is the largest, which is 66.24%, and the inhibition rates of the roots of other plants are all below 20%. The fermentation liquid has no obvious inhibitory effect on the bud growth of the seven forages and crops, and the inhibition rate is all below 15%. In summary, the fermentation liquid has a good inhibitory effect on the seed germination of the seven forages and crops, and the inhibition on the bud growth is the weakest.

[0078] Table 5 Effects of fermentation liquid on seed germination and root / sprout growth of forage and crop seeds

[0079]

[0080] 3. The effect of fermentation liquid on the growth of stems and leaves of forage and crops.

[0081] A total of 15 plants, including 4 kinds of forage grasses (Elymus tinctorius, alfalfa, Sweet clover, and Leymus chinensis) and 11 kinds of crops (corn, soybean, eggplant, tomato, pepper, spinach, cabbage, Chinese cabbage, cucumber, wheat, and oats), were selected as test subjects for the effects of fermentation liquid on the growth of stems and leaves of forage grasses and crops.

[0082] Fifteen kinds of forage grasses and crops were sown in small plastic pots, and the small pots were placed in a tray filled with water, and then placed in an incubator with 18 h light and 6 h dark light for natural growth. When the forage grasses and crops grew to the 3-5 leaf stage, the treatment group sprayed the fermentation liquid prepared in Example 3 evenly on the leaves of the forage grasses and crops until they were covered with dewdrops, and then covered with a black plastic bag for 24 hours in the dark, and sprayed with the bacterial liquid for 3 days continuously. The control group sprayed with sterile water, and each treatment was repeated 3 times. The growth was observed every two days and the data was recorded. The criteria for judging crop disease susceptibility were the same as those in Example 5.

[0083] The results are shown in Table 6. The fermentation liquid has a serious impact on the growth of stems and leaves of corn, soybeans, and cabbage, and also has a slight inhibitory effect on the growth of Elymus dahliae; it is safe for other forage and crops and can be used safely.

[0084] Table 6 Effects of fermentation liquid on the growth of forage and crop stems and leaves

[0085]

[0086] The ability to efficiently control target weeds is the basis for whether the biocontrol bacteria have research value. Epicoccum sorghinum ) TL02 has good biological control effect on weeds such as long-spined tribulus terrestris. The fermentation liquid has a good pathogenic effect on long-spined tribulus terrestris and only affects the growth of corn, cabbage, soybean, and Elymus salsa. It is highly safe for other forage and crops and can be used as a biological herbicide.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Echinococcus sorghumus ( Epicoccumsorghinum ) TL02, characterized in that, Its deposit number is CGMCC No.41277.

2. Sorghum Echinococcus ( Epicoccumsorghinum ) TL02 bacterial agent, characterized in that: It includes the sorghum spore bacteria as claimed in claim 1 ( Epicoccumsorghinum ) TL02 and / or its fermentation broth.

3. Sorghum Echinococcus ( Epicoccumsorghinum ) A method for preparing TL02 fermentation broth, characterized in that: It includes the following steps: 1) Inoculation: The sorghum-attacking bacteria ( Epicoccumsorghinum TL02 was inoculated on PDA solid medium and cultured at 25°C for 7 days to obtain activated strains; 2) Fermentation: Inoculate the activated strain into PDB liquid culture medium and culture at 25°C and 180 rpm; 3) Filtration: Centrifuge the bacterial solution after 14 days of culture, and the supernatant obtained after centrifugation is the fermentation broth.

4. The sorghum-attacking bacteria according to claim 1 ( Epicoccumsorghinum ) TL02 or the sorghum spore bacteria described in claim 2 ( Epicoccumsorghinum ) Application of TL02 microbial agent in controlling long-spined tribulus terrestris.

5. The sorghum-attacking bacteria according to claim 1 ( Epicoccumsorghinum ) TL02 or the sorghum spore bacteria described in claim 2 ( Epicoccumsorghinum ) Application of TL02 microbial agent in the preparation of products for controlling long-spined tribulus terrestris.

6. The sorghum-epiploic bacteria according to claim 1 ( Epicoccumsorghinum ) TL02 or the sorghum spore bacteria described in claim 2 ( Epicoccumsorghinum ) Application of TL02 microbial agent in inhibiting the growth of root shoots of Tribulus terrestris.

7. The sorghum-epiploic bacteria according to claim 1 ( Epicoccumsorghinum ) TL02 or the sorghum spore bacteria described in claim 2 ( Epicoccumsorghinum ) The application of TL02 microbial agent in inhibiting the growth of weeds is characterized in that: The weeds are at least one of Amaranthus retroflexus, Chenopodium album, Echinochloa crusgalli, Chloris viridis, Setaria viridis, and Digitaria sanguinalis.

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