Insecticide composition and its application

Abstract

The present invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition and its use. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B. Active ingredient A is a compound of formula I: #imgabs0#, and active ingredient B is a diamide compound, including any one of flubendiamide, flubendiamide, thiobencarb, chlorocyanamide, and tetrachlorfenapyr. The mass ratio of active ingredient A to active ingredient B is 1:40 to 40:1. The insecticidal composition of the present invention can effectively control lepidopteran pests such as diamondback moth, cabbage looper, and rice stem borer, and has the advantages of significant synergistic effect, expanded insecticide spectrum, reduced application amount, and crop safety.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing technology and discloses an insecticide composition and application thereof. Background Art

[0002] Lepidoptera pests are the second largest order in the class Insecta, boasting a wide variety of species. They are also the most numerous order of agricultural and forestry pests, and pose the greatest threat to crops. There are currently 200,000 species of Lepidoptera pests worldwide, with over 8,000 found in China. Lepidoptera pests primarily feed on plant leaves and stems through larvae, causing symptoms such as leaf chips, hollow holes in leaves and stems, and white ears, hindering crop growth and reducing yield and quality. Lepidoptera pests are difficult to control due to their overlapping generations, high egg production, multiple generations per year, hidden larvae, and rapid development of resistance to common insecticides.

[0003] In order to solve the problems of difficulty in controlling lepidopteran pests and rapid development of drug resistance, the applicant explored the combined effects of the compound of formula I and the diamide compounds polyflubenzuron, flubendiamide, thiobenzuronamide, chloroflucythiazolinone, and tetrachlorfenapyr on lepidopteran pests, aiming to provide a theoretical basis for reducing the amount of pesticides used to control lepidopteran pests and improving the control effect. However, there are currently no reports on the combination of the compound of formula I with polyflubenzuron, flubendiamide, thiobenzuronamide, chloroflucythiazolinone, and tetrachlorfenapyr for the control of lepidopteran pests. Summary of the Invention

[0004] The object of the present invention is to provide an insecticide composition which has synergistic effects, reduces drug resistance, has low use cost and excellent control effect.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: an insecticide composition, wherein the active ingredients of the insecticide composition include active ingredient A and active ingredient B, and the active ingredient A is a compound of formula I: The active ingredient B is a diamide compound, which includes any one of polyflubenzuron diamide, flubendiamide, thiobenzuron amide, chlorofluanid, and tetrachlorantraniliprole. The mass ratio of the active ingredient A to the active ingredient B is 1:40 to 40:1, or any value within the above numerical range.

[0006] Furthermore, the active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 24:1, or any value within the above numerical range;

[0007] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:1, or any value within the above numerical range;

[0008] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 36:1, or any value within the above numerical range;

[0009] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 28:1, or any value within the above numerical range;

[0010] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1, or any value within the above numerical range.

[0011] Furthermore, the active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:8, 1:4, 1:1, 3:2, 4:1, 8:1, 16:1, 24:1, or any value in between;

[0012] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, 15:1, 20:1, 30:1, 40:1, or any value in between;

[0013] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:18, 1:9, 1:3, 3:1, 9:1, 18:1, 25:1, 30:1, 36:1, or any value in between;

[0014] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:28, 1:12, 1:8, 2:1, 8:1, 12:1, 20:1, 28:1, or any value in between;

[0015] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20, 1:10, 1:5, 2:1, 5:1, 10:1, 20:1, 30:1, or any value between the above values.

[0016] Furthermore, the active ingredient B is polyflubenzuronil, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 16:1, or any value within the above numerical range;

[0017] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 30:1, or any value within the above numerical range;

[0018] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 30:1, or any value within the above numerical range;

[0019] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 20:1, or any value within the above numerical range;

[0020] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1, or any value within the above numerical range;

[0021] Furthermore, the active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15, 1:8, 1:4, 1:1, 3:2, 4:1, 8:1, 16:1, or any value in between;

[0022] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:5, 1:2, 5:1, 10:1, 15:1, 20:1, 30:1, or any value in between;

[0023] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:18, 1:9, 1:3, 3:1, 9:1, 18:1, 25:1, 30:1, or any value in between;

[0024] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:28, 1:12, 1:8, 2:1, 8:1, 12:1, 20:1, or any value in between;

[0025] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:5, 2:1, 5:1, 10:1, 20:1, or any value between the above values.

[0026] Furthermore, the active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 8:1, or any value within the above numerical range;

[0027] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 20:1, or any value within the above numerical range;

[0028] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:9 to 25:1, or any value within the above numerical range;

[0029] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 12:1, or any value within the above numerical range;

[0030] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 10:1, or any value within the above numerical range.

[0031] Furthermore, the active ingredient B is polyflubenzuronil, and the mass ratio of the active ingredient A to the active ingredient B is 1:15, 1:8, 1:4, 1:1, 3:2, 4:1, 8:1, or any value in between;

[0032] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:5, 1:2, 5:1, 10:1, 15:1, 20:1, or any value in between;

[0033] The active ingredient B is thiobencarb, and the mass ratio of the active ingredient A to the active ingredient B is 1:9, 1:3, 3:1, 9:1, 18:1, 25:1, or any value in between;

[0034] The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:12, 1:8, 2:1, 8:1, 12:1, or any value in between;

[0035] The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:5, 2:1, 5:1, 10:1, or any value between the above values.

[0036] Furthermore, the total weight of the insecticide composition is expressed as 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 70% of the total weight of the insecticide composition.

[0037] Furthermore, the insecticide composition contains auxiliary ingredients in addition to the active ingredient, and the auxiliary ingredients are one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0038] Furthermore, the wetting agent is selected from a mixture of one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, sapodilla powder, silkworm excrement or soapberry powder;

[0039] Furthermore, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ether phosphates, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0040] Furthermore, the emulsifier is selected from Nongru 500# (calcium alkylbenzene sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonic acid magnesium salt, triethanolamine salt, Nongru 400# (benzyl dimethylphenol polyoxyethyl ether), Nongru 700# (alkylphenol formaldehyde resin polyoxyethyl ether), Ningru 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), A mixture of one or more of the following: Nongru 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), Nongru 33# (alkylaryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether);

[0041] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0042] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0043] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0044] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0045] Furthermore, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

[0046] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0047] Furthermore, the penetrant is selected from a mixture of one or more of penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone or silicone;

[0048] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0049] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0050] The above substances are all commercially available.

[0051] Furthermore, the formulation of the insecticide composition is a solid preparation or a liquid preparation.

[0052] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0053] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0054] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0055] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion, an aqueous emulsion, a suspension or a dispersible oil suspension.

[0056] The present invention also discloses the use of the above-mentioned insecticide composition for preventing and controlling agricultural, forestry or horticultural lepidopteran pests;

[0057] Furthermore, the lepidopteran pests include diamondback moth, beet armyworm, Spodoptera litura, striped stem borer, cotton bollworm or cabbage worm.

[0058] In order to obtain the desired insecticidal effect, the dosage of the pesticide composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0059] The beneficial effects of the present invention are as follows:

[0060] 1) The insecticide composition of the present invention is compounded with agents having different mechanisms of action in a reasonable ratio, and has a significant synergistic effect on pests within an appropriate mass ratio range;

[0061] 2) The insecticidal composition of the present invention has excellent control effects on lepidopteran pests in agriculture, horticulture and forestry;

[0062] 3) The insecticide composition of the present invention slows down the development of insecticide resistance, and is particularly effective against diamondback moth, cabbage looper, and striped stem borer, thereby reducing the amount of pesticides used and alleviating environmental pressure. DETAILED DESCRIPTION

[0063] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0064] Preparation example:

[0065] Preparation Example 1: 20% Formula I compound·polyflubendiamide suspension (1:4)

[0066] Formula: 4% compound of formula I, 16% polyflubenzuronide, 1% sodium lauryl sulfate, 2% sodium alkyl polyoxyethylene ether sulfonate, 4% polyoxyethylene sorbitan monooleate, 4% alkylphenol polyoxyethylene ether phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0067] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0068] Preparation Example 2: 30% Formula I compound·polyflubendiamide water dispersible granules (3:2)

[0069] Formula: 18% compound of formula I, 12% polyflubenzuronide, 8% lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 5% white carbon black, 25% starch, and kaolin makes up the balance;

[0070] Preparation method: According to the proportions in the example, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the mixture is kneaded, granulated, dried, and sieved to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0071] Preparation Example 3: 18% Formula I compound·polyflubendiamide dispersible oil suspension (8:1)

[0072] Formula: 16% compound of formula I, 2% polyflubenzuronide, 1% succinic acid sulfonate, 2% isomeric tridecyl alcohol polyoxyethylene ether, 13% triphenyl phenol polyoxyethylene ether, 4% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% silicon dioxide, 1% organic bentonite, and methyl oleate makes up the balance;

[0073] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0074] Preparation Example 4: 24% Formula I compound·flubenzuronil suspension (1:5)

[0075] Formula: 4% compound of formula I, 20% flubendiamide, 1% Guerbet alcohol polyoxyethylene ether, 5% polyoxyethylene sorbitan monooleate, 1% sodium lignin sulfonate, 4% alkylphenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% sodium sorbate, 0.5% silicone oil, and deionized water to make up the balance;

[0076] Preparation method: Same as Preparation Example 1.

[0077] Preparation Example 5: 22% Formula I compound·flubenzuronil water dispersible granules (10:1)

[0078] Formula: 20% compound of formula I, 2% flubendiamide, 10% sodium lignin sulfonate, 2% lakai powder BX, 6% sodium polycarboxylate, 5% white sugar, and kaolin makes up the balance.

[0079] Preparation method: same as Preparation Example 2.

[0080] Preparation Example 6: 30% Formula I compound·flubenzuronide wettable powder (5:1)

[0081] Formula: 25% compound of formula I, 5% flubendiamide, 5% sodium lignin sulfonate, 2% sodium dioctyl sulfosuccinate, 8% dispersant NNO, 2% pulverizing powder BX, 5% white carbon black, and kaolin to make up the balance;

[0082] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring kettle, and pulverized and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0083] Preparation Example 7: 30% Formula I compound·sulfinamide suspension (1:9)

[0084] Formula: 3% compound of formula I, 27% thiobenzamide, 2% Guerbet alcohol polyoxyethylene ether, 3% sorbitan oleate polyoxyethylene ether, 3% styrenated phenol polyoxyethylene ether sulfate, 1% sodium salt of polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.25% silicone oil, and deionized water to make up the balance;

[0085] Preparation method: Same as Preparation Example 1.

[0086] Preparation Example 8: 38.5% Formula I compound·sulfuronamide water dispersible granules (10:1)

[0087] Formula: 35% compound of formula I, 3.5% thiobenzamide, 2% succinate sulfonate, 4% naphthalenesulfonate formaldehyde condensate, 12% calcium lignin sulfonate, 2% sodium lauryl sulfate, 10% ammonium sulfate, and starch makes up the balance.

[0088] Preparation method: same as Preparation Example 2.

[0089] Preparation Example 9: 20% Formula I compound·sulfinamide dispersible oil suspension (1:3)

[0090] Formula: 5% compound of formula I, 15% thiobenzamide, 5% alkylaryl polyoxyethylene ether polyoxypropylene ether, 8% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% sodium salt of polycarboxylate, 0.5% organic bentonite, and soybean oil makes up the balance;

[0091] Preparation method: Same as Preparation Example 3.

[0092] Preparation Example 10: 18% Formula I compound·Cyfluanid suspension concentrate (1:8)

[0093] Formula: 2% compound of formula I, 16% chlorfluanid, 2% tristyrylphenol polyoxyethylene ether, 1% naphthalenesulfonate formaldehyde condensate, 3% tristyrylphenol ethoxylate phosphate, 3% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0094] Preparation method: Same as Preparation Example 1.

[0095] Preparation Example 11: 20% Formula I compound·Cyfluanid water dispersible granules (9:1)

[0096] Formula: 18% compound of formula I, 2% chlorfluanid, 3% wetting agent EFW, 7% sodium lignin sulfonate, 8% naphthalene sulfonate formaldehyde condensate, 5% white carbon black, 25% starch, and kaolin as the balance.

[0097] Preparation method: same as Preparation Example 2.

[0098] Preparation Example 12: 42% Formula I compound·Cyanoflurane wettable powder (2:1)

[0099] Formula: 28% compound of formula I, 14% chlorfluanid, 2% sodium lauryl sulfate, 8% naphthalenesulfonate formaldehyde condensate, 5% tea saponin, 2% sodium alginate, 8% light calcium carbonate, and kaolin makes up the balance;

[0100] Preparation method: Same as Preparation Example 6.

[0101] Preparation Example 13: 27% Formula I compound·tetrachlorantraniliprole suspension (1:5)

[0102] Formula: 4.5% compound of formula I, 22.5% tetrachlorantraniliprole, 5% ethylene glycol oxyethylene polyoxypropylene ether, 5% tristyrylphenol ethoxylate phosphate, 2% sodium polycarboxylate, 0.25% xanthan gum, 5% glycerol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0103] Preparation method: Same as Preparation Example 1.

[0104] Preparation Example 14: 30% Formula I compound·tetrachlorantraniliprole water dispersible granules (1:1)

[0105] Formula: 15% compound of formula I, 15% tetrachlorantraniliprole, 10% sodium lignin sulfonate, 2% succinate sulfonate, 6% naphthalenesulfonate formaldehyde condensate, 8% ammonium sulfate, and kaolin makes up the balance;

[0106] Preparation method: same as Preparation Example 2.

[0107] Preparation Example 15: 11% Formula I compound·tetrachlorantraniliprole emulsifiable concentrate (10:1)

[0108] Formula: 10% compound of formula I, 1% tetrachlorantraniliprole, 15% propylene glycol methyl ether, 12% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 12% DMF, 20% cyclohexanone, and methyl oleate makes up the balance;

[0109] Preparation method: according to the formula ratio, the active ingredient, solvent and cosolvent are added to the mixing kettle and stirred to dissolve, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in the stirring kettle and filtered to obtain the desired emulsifiable concentrate of the present invention.

[0110] Example 1: Indoor biological activity assay

[0111] Test target: Second-instar larvae of Pieris rapae;

[0112] Test agents: compound of formula I, flubendiamide, flubendiamide, thiobencarb, chlorfluanid, and tetrachloranid;

[0113] Preparation of pharmaceutical preparation: Dissolve the above raw drugs in a suitable solvent, and then prepare them into 5 mass concentration gradients according to the equal proportion method using 0.1% Tween-80 aqueous solution.

[0114] Test insect age: Select healthy and consistent second-instar test insects, use tweezers to place fresh cabbage leaves that have not been treated with drugs in the drug solution and immerse them for 10 seconds, then take them out. After the drug solution dries naturally, place the cabbage pieces in a culture dish lined with moisturizing filter paper, use the filter paper to absorb the excess drug solution, and place the test insects in the culture dish with the immersed leaves.

[0115] Each treatment was replicated four times, with 20 test insects per replicate. A blank treatment was used as a control. The treated test insects were reared in an artificial intelligence culture chamber at 26±1°C, a light intensity of L:D = 16h:8h, and a relative humidity of 60%.

[0116] After treatment with the pesticide, the mortality of the test insects was investigated 48 hours later. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0117] Data statistics and analysis:

[0118] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula:

[0119]

[0120] Where:

[0121] P——mortality rate, in percentage (%);

[0122] K——indicates the number of dead insects, the unit is head;

[0123] N——represents the total number of insects processed, in heads.

[0124]

[0125] Where:

[0126] P1——adjusted mortality rate, in percentage (%);

[0127] P t ——Treatment mortality rate, expressed in percentage (%);

[0128] P0 - blank control mortality rate, in percentage (%).

[0129] Data statistics and analysis: If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the experiment needs to be repeated.

[0130] Use DPS statistical analysis system to analyze and find the toxicity regression line and LC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient R.

[0131] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0132]

[0133] Where:

[0134] ATI - measured toxicity index of mixture;

[0135] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0136] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0137] TTI=TI A ×P A +TI B ×P B

[0138] Where:

[0139] TTI – Theoretical Toxicity Index of Mixtures;

[0140] TI A ——Agent toxicity index;

[0141] P A——The percentage of agent A in the mixture, in percentage (%);

[0142] TI B ——Toxicity index of agent B;

[0143] P B ——The percentage of agent B in the mixture, in percentage (%).

[0144]

[0145] Where:

[0146] CTC – Co-toxicity coefficient;

[0147] ATI - measured toxicity index of mixture;

[0148] TTI - Theoretical Toxicity Index of Mixture.

[0149] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The test results are as follows:

[0150] Table 1 Indoor biological activity test of compound I and polyflubendiamide against Pieris rapae

[0151]

[0152] Indoor test results showed that combining the compound of Formula I with polyflubendiamide within an appropriate mass ratio range exhibited excellent control effects against Pieris rapae. When the mass ratio of the compound of Formula I to polyflubendiamide was 1:25 to 24:1, the co-toxicity coefficient against Pieris rapae was greater than 120, demonstrating a synergistic effect. When the mass ratio of the compound of Formula I to polyflubendiamide was 1:15 to 8:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to polyflubendiamide was 1:8 to 4:1, the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect.

[0153] Table 2 Indoor biological activity test of compound of formula I and flubendiamide against Pieris rapae

[0154]

[0155] Indoor test results showed that combining the compound of Formula I with flubendiamide within an appropriate mass ratio range exhibited excellent control effects against Pieris rapae. When the mass ratio of the compound of Formula I to flubendiamide was 1:20 to 40:1, the co-toxicity coefficient against Pieris rapae was greater than 120, demonstrating a synergistic effect. When the mass ratio of the compound of Formula I to flubendiamide was 1:10 to 30:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to flubendiamide was 1:5 to 30:1, the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect.

[0156] Table 3 Indoor biological activity test of compound I combined with thiobencarb against Pieris rapae

[0157]

[0158] Laboratory test results show that combining the compound of Formula I with thiobencarbamide within an appropriate mass ratio range exhibits excellent control efficacy against Pieris rapae. When the mass ratio of the compound of Formula I to thiobencarbamide is 1:25 to 36:1, the co-toxicity coefficient against Pieris rapae is greater than 120, demonstrating a synergistic effect. When the mass ratio of the compound of Formula I to thiobencarbamide is 1:9 to 30:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to thiobencarbamide is 1:9 to 25:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0159] Table 4 Indoor biological activity test of compound of formula I and cyfluthrin against Pieris rapae

[0160]

[0161] Laboratory test results showed that combining the compound of Formula I with chlorfluanid within an appropriate mass ratio range demonstrated excellent control efficacy against Pieris rapae. When the mass ratio of the compound of Formula I to chlorfluanid was 1:35 to 28:1, the co-toxicity coefficient against Pieris rapae was greater than 120, demonstrating a synergistic effect. When the mass ratio of the compound of Formula I to chlorfluanid was 1:28 to 20:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to chlorfluanid was 1:12 to 12:1, the co-toxicity coefficient was greater than 140, demonstrating a significant synergistic effect.

[0162] Table 5 Indoor biological activity test of compound of formula I and tetrachlorantraniliprole against Pieris rapae

[0163]

[0164] Laboratory test results show that combining the compound of Formula I with chlorantraniliprole within an appropriate mass ratio range exhibits excellent control efficacy against Pieris rapae. When the mass ratio of Formula I to chlorantraniliprole is 1:20 to 30:1, the co-toxicity coefficient against Pieris rapae is greater than 120, demonstrating a synergistic effect. When the mass ratio of Formula I to chlorantraniliprole is 1:10 to 20:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of Formula I to chlorantraniliprole is 1:5 to 10:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0165] Example 2: Field efficacy test on Pieris rapae and Plutella xylostella

[0166] Test basis: The test refers to GB / T 17980.13-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling lepidopteran larvae of cruciferous vegetables".

[0167] Test targets: Pieris rapae and Plutella xylostella.

[0168] Experimental crop: Cabbage.

[0169] The experimental site was a cabbage field in Taipingqiao Village, Bixi Street, Changshu City, Suzhou City, Jiangsu Province. The field management level and soil fertility of all experimental plots in the experimental site were consistent, which was in line with local scientific production practices.

[0170] Experimental plot arrangement: The experimental drug, control drug and blank control plots were arranged in random blocks, with each plot area of ​​20m 2 , each treatment was repeated 4 times.

[0171] Application time and method: The test was conducted on May 7, 2023, and the cabbage leaves were evenly sprayed with a water consumption of 675 kg / hm 2 No other insecticides were used during the trial. A 3WBS-16B backpack manual sprayer was used for application. The spray was required to be uniform and consistent, with no overlap or omissions.

[0172] Efficacy investigation: Investigate the insect population base before application, investigate the number of diamondback moth and cabbage looper larvae on the whole leaves 5 and 7 days after application, randomly investigate 4 points for each treatment, 5 plants at each point, for a total of 20 plants, repeat 4 times and take the average value to calculate the control effect.

[0173] The calculation formula is as follows:

[0174]

[0175]

[0176] The results of the field efficacy test are shown in the following table:

[0177] Table 5 Field efficacy test on control of cabbage looper and diamondback moth

[0178]

[0179]

[0180] Safety investigation: During the field trial, no pesticide damage occurred in any treatment group, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area, indicating that the test agents were safe for cabbage growth at the doses provided.

[0181] The results of the field efficacy test showed that 5 days and 7 days after application, the quick-acting and long-lasting effects of the compound preparations in controlling cabbage loopers and diamondback moths were significantly better than those of other pesticides.

[0182] Example 3: Field efficacy test on Chilo suppressalis

[0183] Test basis: The test refers to GB / T 17980.1-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling rice lepidopteran borers".

[0184] Test target: Rice stem borer;

[0185] Test location: The test field is located in Shuangqiao Village, Dingshu Town, Yixing City, Jiangsu Province. The soil organic matter content is medium, the soil fertility is above medium, and the previous crop is wheat.

[0186] Experimental plot arrangement: The experiment was conducted with 12 treatments based on different pesticides, with the same amount of liquid pesticide used as the blank control. Each treatment was repeated 4 times, and the area of ​​each plot was 50m 2 The specific pesticide regimen for each treatment is shown in the table below. During the experiment, no other pesticides were applied to any treatment, and other cultivation management remained the same.

[0187] Application time: The experiment was conducted on June 2, 2023, when the rice was in the tillering stage. The application device was a Shandong Weishi WS-16 backpack manual sprayer with a spray operating pressure of 0.2-0.3 MPa and a nozzle diameter of 1.0 mm.

[0188] Survey Method: Survey the insect population before application, and conduct a control efficacy survey after the current infestation is established (20 days after application). The survey uses a parallel jump sampling method at five points, with five consecutive clumps surveyed at each point, and 25 clumps surveyed per plot. The number of dead rice cores was counted, and the control efficacy was calculated.

[0189] The calculation formula is as follows:

[0190]

[0191]

[0192] The results of the field efficacy test are shown in the following table:

[0193] Table 6 Field efficacy test for controlling Chilo suppressalis

[0194]

[0195]

[0196] Safety Survey Results: Regular observations after application showed that rice plants in all treatment areas grew normally, with no significant abnormalities such as discoloration, deformities, or yellowing of leaf tips. Plant height and leaf color were similar to those in the water control area, demonstrating that all tested pesticides were safe for rice growth.

[0197] 20 days after application, 30% Formula I compound·flubenzuron amide wettable powder (5:1), 20% Formula I compound·chlorfluanid water dispersible granules (9:1), 30% Formula I compound·polyflubenzuron diamide water dispersible granules (3:2), 20% Formula I compound·sulfuranid dispersible oil suspension (1:3), and 11% Formula I compound·tetrachloranid emulsifiable concentrate (10:1) all had good control effects on the striped stem borer. Among them, the group treated with 30% Formula I compound·flubenzuron amide wettable powder (5:1) had the lowest heart rate of 0.73%, and the best control effect of 97.19%.

[0198] In summary, through indoor toxicity assays and field efficacy tests, it can be seen that the insecticidal composition of the present invention has a good control effect on Lepidoptera and pests, is safe for target crops, has significant control effects, and is superior to a single agent in delaying the development of drug resistance and prolonging the effectiveness.

[0199] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition, characterized in that The active ingredients of the insecticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I: (Formula I), the active ingredient B is a bisamide compound, and the bisamide compound includes any one of polyflubendiamide, flubendiamide, thiobencarb, chlorfluanid, and tetrachlorantraniliprole; The active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25-24:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:1; The active ingredient B is thiobenzamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25-36:1; The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 28:1; The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20-30:

1.

2. The insecticidal composition according to claim 1, characterized in that The active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15-16:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 30:1; The active ingredient B is thiobenzamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 30:1; The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 20:1; The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:

1.

3. The insecticidal composition according to claim 2, characterized in that The active ingredient B is polyflubenzuronamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 8:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 20:1; The active ingredient B is thiobenzamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:9 to 25:1; The active ingredient B is chlorfluanid, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 12:1; The active ingredient B is tetrachlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 10:

1.

4. The insecticidal composition according to claim 1, characterized in that The total weight of the insecticide composition is expressed as 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 70% of the total weight of the insecticide composition.

5. The insecticidal composition according to claim 1, characterized in that The insecticide composition contains auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The insecticidal composition according to claim 1, characterized in that The formulation of the insecticide composition is a solid preparation or a liquid preparation.

7. The insecticidal composition according to claim 6, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion, an aqueous emulsion, a suspension or a dispersible oil suspension.

8. Use of the insecticidal composition according to any one of claims 1 to 7 for controlling agricultural, forestry or horticultural lepidopteran pests.

9. The use according to claim 8, characterized in that The lepidopteran pests include diamondback moth, beet armyworm, prodenia litura, striped stem borer, cotton bollworm or cabbage worm.

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