A pesticide composition against plant viruses and use thereof

Abstract

The present application provides a kind of pesticide composition for resisting plant virus and its application.The pesticide composition is made of raw materials including the following components: flubendiamide and amplex. The mass ratio of flubendiamide and amplex is 31:1-1:1. Compared with flubendiamide single agent, the composition of the present application can significantly improve the use effect of flubendiamide, and can greatly reduce the dosage under the condition of the same prevention effect. Flubendiamide and amplex have synergistic effect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of pesticides, and particularly relates to a pesticide composition for resisting plant viruses and application thereof. BACKGROUND

[0002] Fluorobenzyl thiofurfuryl ether (development code GZU-001, CAS: 2088490-68-8) is a new generation of plant immune inducer developed by Guizhou University based on natural product vanillin. CN106467478A discloses that fluorobenzyl thiofurfuryl ether can improve the resistance of tobacco to viral diseases, but has no direct destructive effect on tobacco viruses. CN106467478A discloses that it also has certain plant growth regulating activity.

[0003]

[0004] Viral diseases are also known as plant "cancer", and are a relatively difficult problem in the field of plant protection. Although CN106467478A discloses that some compounds have better anti-disease effects than ningnanmycin, which is a widely used anti-virus agent on the market, but the method for improving the efficacy and reducing the dosage is still a direction that needs to be researched by technical personnel. SUMMARY

[0005] The application aims to provide a pesticide composition for resisting plant viruses.

[0006] The pesticide composition for resisting plant viruses provided by the application is made of raw materials including the following components: fluorobenzyl thiofurfuryl ether and profenofen.

[0007] In the composition, the mass ratio of fluorobenzyl thiofurfuryl ether and profenofen can be 31:1-1:1, specifically, the mass ratio of fluorobenzyl thiofurfuryl ether and profenofen can be 20:1-1:0.8, 20:1-5:1, 20:1-5:2, 20:1-10:1, 10:1-5:1, 10:1-5:2, more specifically, 20:1, 15:1, 10:1, 5:1, 5:2, 5:4, 7:1, 3:1.

[0008] The plant viruses can be tobacco mosaic virus, potato virus Y, and cucumber mosaic virus.

[0009] In addition to containing fluorobenzyl thiofurfuryl ether and profenofen, the pesticide composition can also be processed into various dosage forms by adding an auxiliary agent (such as a surfactant, a carrier).

[0010] The dosage form can be aqueous solution, water emulsion, wettable powder, soluble powder, soluble granule, water dispersible granule, suspension, soluble solution, oil suspension, tablet, or effervescent granule.

[0011] The effective component in the dosage form can have a suitable concentration of 0.1-60 wt%, specifically 0.5-15 wt%, 0.6-5.1 wt%, 2-52 wt%, or 11-21 wt%.

[0012] The use of the complex component can not only improve the prevention and treatment effect of viral diseases, but also expand the use space of the composition. Especially under the prevention and treatment principle of "prevention first", the component is more easily accepted by the market if it is applied in advance. DETAILED DESCRIPTION

[0013] The application will be further described in detail below with specific embodiments. The examples provided below are only for illustrating the application, and are not intended to limit the scope of the application. The examples provided below can serve as a guide for further improvement by those skilled in the art, and do not constitute any limitation on the application.

[0014] In the following examples, the experimental methods are conventional methods, and are performed according to the techniques or conditions described in the literature in the art or according to the product instructions, unless otherwise specified. The materials, reagents, etc. used in the following examples can be obtained from commercial channels, unless otherwise specified.

[0015] Example 1, preliminary activity test

[0016] The experiment was performed according to "NY / T 1156.6-2006 Pesticide Indoor Biological Assay Experiment Standard". The semi-leaf spot method was used to carry out the screening of the synergistic formula with heart leaf tobacco as the experimental material.

[0017] The TMV virus liquid was provided by the Fine Chemical Research Center of Guizhou University and was stored in a freezer.

[0018] Experimental reagents: 95% flubendiamide technical material (CN109134327A, provided by Hebi Quanfeng Biological Technology Co., Ltd.), 98% amplexid technical material (PD20184001, Hebi Quanfeng Biological Technology Co., Ltd.)

[0019] Test method: Select heart leaf tobacco with consistent growth at the 5-6 leaf stage, and trim the plant body, remove old leaves, and retain 3-4 healthy leaves. Use a brush to dip different treated reagents on the left half leaf, and dip the right half leaf with the corresponding concentration of solvent as a control. After 24 hours, evenly sprinkle the whole leaf with emery powder, dilute the TMV virus liquid 500 times, and use a brush to dip the virus liquid for whole leaf virus inoculation. After 30 minutes, rinse with water, and investigate when the disease spots are obvious (usually 72h). Record the number of disease spots on the left and right leaves, and calculate the inhibition rate according to the following formula:

[0020]

[0021] Wherein, the average number of dead spots is the average number of each group of three repetitions.

[0022] The results of the bioassay are shown in Table 1:

[0023] Table 1, results of preliminary activity test

[0024]

[0025] From the above preliminary screening activity of the monoamyl ester of 2-(2-oxoethyl) 1,3- dimethylimidazolinium chloride, it can be seen that the addition of monoamyl ester of 2-(2-oxoethyl) 1,3- dimethylimidazolinium chloride improves the protection effect of the fluazuron on tobacco mosaic virus, but the degree of synergism and specific technical parameters still need further experimental exploration.

[0026] Example 2, synergistic screening experiment

[0027] The experiment adopts the half-leaf dead spot method for screening test, and the specific conditions are referred to in Example 1. DPS is used for data processing, and Sun Yunpei method is used for evaluating the synergistic effect.

[0028] Table 2, results of synergistic experiment

[0029]

[0030] From the above data, it can be seen that the addition of monoamyl ester of 2-(2-oxoethyl) 1,3- dimethylimidazolinium chloride improves the protection effect of the fluazuron on tobacco mosaic virus. When fluazuron and monoamyl ester of 2-(2-oxoethyl) 1,3-dimethylimidazolinium chloride are compounded at a ratio of 20:1, 10:1 and 5:1 respectively, the co-toxicity coefficients are all greater than 120, indicating that monoamyl ester of 2-(2-oxoethyl) 1,3-dimethylimidazolinium chloride has a synergistic effect on fluazuron.

[0031] Example 3, synergistic screening experiment

[0032] According to the above experimental results, the synergistic range of fluazuron and monoamyl ester of 2-(2-oxoethyl) 1,3-dimethylimidazolinium chloride is explored. The experiment adopts the half-leaf dead spot method for screening test, and the specific conditions are referred to in Example 1. DPS is used for data processing, and Sun Yunpei method is used for evaluating the synergistic effect.

[0033] Table 3, results of synergistic experiment

[0034]

[0035]

[0036] From the above results, it can be seen that the compatibility of fluorine benzyl thiofuran and profenofen shows certain synergistic effect on TMV. Among them, when the ratio of fluorine benzyl thiofuran to profenofen is 5:8, the CTC value is between 80-120, which is additive effect, and when the ratio of fluorine benzyl thiofuran to profenofen is 5:2 and 5:4, the CTC value is greater than 120, which is synergistic effect. Combined with the previous experiment, it can be seen that in the process of fluorine benzyl thiofuran and profenofen preventing and treating viral diseases, fluorine benzyl thiofuran is the main effective factor. From the above experiments, it can be seen that in the compatibility of fluorine benzyl thiofuran and profenofen, when the ratio of fluorine benzyl thiofuran to profenofen is from 1.25 to 0.625, the effect changes from synergistic to additive, and the use of profenofen is too high, which has limited effect on the improvement of antiviral effect. In addition, when the dosage of profenofen is 160 mg / L, the growth of plants is inhibited, which shows that this dosage is not suitable for use.

[0037] From the above results, it can be seen that the compatibility of fluorine benzyl thiofuran and profenofen shows certain synergistic effect on TMV. Among them, when the ratio of fluorine benzyl thiofuran to profenofen is 5:8, the CTC value is between 80-120, which is additive effect, and when the ratio of fluorine benzyl thiofuran to profenofen is 5:2 and 5:4, the CTC value is greater than 120, which is synergistic effect. Combined with the previous experiment, it can be seen that in the process of fluorine benzyl thiofuran and profenofen preventing and treating viral diseases, fluorine benzyl thiofuran is the main effective factor. From the above experiments, it can be seen that in the compatibility of fluorine benzyl thiofuran and profenofen, when the ratio of fluorine benzyl thiofuran to profenofen is from 1.25 to 0.625, the effect changes from synergistic to additive, and the use of profenofen is too high, which has limited effect on the improvement of antiviral effect. In addition, when the dosage of profenofen is 160 mg / L, the growth of plants is inhibited, which shows that this dosage is not suitable for use.

[0038] Example 4, Anti-potato Y virus experiment

[0039] The experiment was carried out according to the "Pesticide indoor biological determination experiment standard NY / T1156.6-2006". The semi-leaf spot method was used, and amaranthus as the experimental material was used to carry out the screening work of synergistic formula.

[0040] PVY virus liquid was provided by Guizhou University Fine Chemical Research Center.

[0041] Experimental reagents: 95% fluorine benzyl thiofuran technical (CN109134327A, provided by Hebi Quantong Biological Technology Co., Ltd.), 98% profenofen technical (PD20184001, Hebi Quantong Biological Technology Co., Ltd.)

[0042] Test method: Select 5-6 leaf stage amaranthus with consistent growth, remove old leaves, and reserve 3 healthy leaves. Dip different treatments of reagents in the left half leaf with a brush, and the right half leaf is coated with the corresponding concentration of solvent as a control. After 24 hours, evenly sprinkle the whole leaf with corundum, dilute the PVY virus liquid 300 times, and use a pen to dip the virus liquid for whole leaf virus inoculation. Thirty minutes later, rinse with water, and investigate when the disease spot is obvious (usually 72h). Record the number of disease spots on the left and right leaves, and calculate the inhibition rate according to the following formula:

[0043]

[0044] Among them, the average number of spots is the average of three repeats in each group.

[0045] The biological determination results are shown in Table 4.

[0046] Table 4, anti-potato Y virus test results

[0047]

[0048] From the above results of the effect against PVY virus, it can be seen that the effect of the compound of dimethomorph and prohexadione-calcium is better than that of dimethomorph alone. The scheme of TMV as a screening object is verified on PVY.

[0049] The synergistic screening experiment of the present application determines that dimethomorph and prohexadione-calcium have good synergistic effect in the range of 20:1 to 1:0.8.

[0050] Although the CTC value can reflect the strength of the synergistic effect between drugs, in actual agricultural prevention and control work, the final prevention and control effect still needs to be considered.

[0051] Example 5, cucumber virus disease prevention and control experiment

[0052] Test conditions: When summering cucumbers face high temperature and drought environment, they are extremely prone to virus diseases. The test was carried out in a vegetable greenhouse in Luocheng Street, Shouguang City, Weifang City, and the greenhouse area was 10m*67m. Cucumbers were planted on May 31, with a row distance of 60cm and a plant distance of 25cm. The previous crop was cucumber.

[0053] Test reagents: 95% dimethomorph technical material (CN109134327A, provided by Hebi Quantong Biological Technology Co., Ltd.), 98% prohexadione-calcium technical material (PD20184001, Hebi Quantong Biological Technology Co., Ltd.)

[0054] Table 5, test design and arrangement

[0055]

[0056]

[0057] A small amount of DMSO was used to dissolve the above technical material, and a 0.1% Tween 80 aqueous solution was used to configure the technical material into a 2000mg / L mother liquor, and then diluted into the required concentration of liquid medicine according to the proportion. Each treatment has 4 repeats, and each repeat is treated with a single row of 8m. The test was sprayed on July 23, and the virus disease prevention and control effect was investigated after 10 days.

[0058] Single plant disease classification standard:

[0059] 0 level: no disease symptoms on the whole plant;

[0060] 1 level: a few yellowing spots appear on the leaves or the central leaf veins are clear;

[0061] 3 level: mosaic on middle and upper leaves;

[0062] 5: middle and upper leaves with heavy leaf curl, a few leaves with crinkled or abnormal shape;

[0063] 7: most leaves with heavy leaf curl, some leaves with crinkled, abnormal shape or necrotic spots, and the plant slightly dwarfed;

[0064] 9: most leaves with heavy leaf curl, abnormal shape or necrotic spots, and the plant obviously dwarfed or even dead.

[0065] Disease index = ∑ number of diseased plants x representative value of the disease level / total number of plants surveyed x representative value of the highest level x 100

[0066]

[0067] Table 6, results and analysis

[0068]

[0069]

[0070] The foregoing test has shown that the addition of the ME has a weak effect on enhancing the plant's ability to resist viruses, but this effect is related to the positive effect of the ME as a plant growth regulator on the growth of the plant, and the weak effect cannot be used as an antiviral agent, and a high dose of the ME can cause phytotoxicity. In order to further verify the synergistic effect of the ME on the thiacloprid, we carried out screening and verification in a cucumber greenhouse. The test results show that the addition of the ME has a significant synergistic effect on the thiacloprid. Under the condition that the effective components are unchanged, the addition of the ME can improve the control effect of the thiacloprid on viral diseases. In the range of 31:1-1:1 in the present test, the ME shows a good improvement effect, and the synergistic effect is obvious at 31:1, 15:1, 7:1 and 1:1. When the total effective component is 160 mg / L, the mass ratio of the thiacloprid to the ME is in the range of 31:1-7:1, the control effect is more than 75.0%, which is significantly higher than that of the thiacloprid single agent at the same dose.

[0071] The present application has been described in detail. For those skilled in the art, without departing from the purpose and scope of the present application, and without unnecessary experiments, the present application can be implemented in a wide range under the same parameters, concentrations and conditions. Although the present application gives a special example, it should be understood that the present application can be further improved. In summary, according to the principle of the present application, the present application intends to include any change, use or improvement of the present application, including changes made by conventional techniques known in the art, which are out of the scope disclosed in the present application.

Claims

1. A pesticide composition made from raw materials comprising the following components: flubenzyl thiophanate and aminoethyl ester; In the composition, the mass ratio of flubenzyl thiocyanate to aminoethyl ester is 31:1 to 1:

1.

2. The pesticide composition according to claim 1, characterized in that, In the composition, the mass ratio of flubenzyl thiocyanate to aminoethyl ester is 20:1 to 1:0.

8.

3. The pesticide composition according to claim 1, characterized in that, In addition to containing flubenzyl thiocyanate and aminoethyl ester, the pesticide composition is also processed into various formulations by adding adjuvants.

4. The pesticide composition according to claim 3, characterized in that, The dosage form is an aqueous solution, an emulsion, a wettable powder, a soluble powder, a soluble granule, a water-dispersible granule, a suspension, a soluble solution, an oil suspension, a tablet, or an effervescent granule.

5. The pesticide composition according to claim 3, characterized in that, The concentration of the active ingredient in the dosage form is 0.1–60 wt%.

6. The application of the pesticide composition according to any one of claims 1-5 in the treatment of plant viruses; wherein the plant virus is: tobacco mosaic virus, potato virus Y, or cucumber mosaic virus.

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