Bactericidal composition, bactericide and application for preventing and controlling eggplant gray mold

Abstract

The present invention discloses a fungicide composition, fungicide, and application for preventing and controlling eggplant gray mold. The active ingredients of the fungicide composition are a compound of fenoxam and zoxamidin. The fungicide, including the fungicide composition, can be used to prevent and control eggplant gray mold. The fungicide composition exhibits a synergistic effect on inhibiting the growth of Botrytis cinerea hyphae, thereby enhancing the inhibitory effect on the pathogen and thereby improving the control of eggplant gray mold. It can also provide support for the screening and development of new control agents.

Description

[0001] This invention is a divisional application of the applicant's invention patent application with application number 202310516172.3 filed on May 9, 2023, and invention name "Fungicide composition, fungicide and application for preventing and controlling eggplant gray mold". Technical Field

[0002] The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and controlling eggplant gray mold. Background Art

[0003] Eggplant gray mold, caused by the fungus Botrytis cinerea Per., is a major eggplant disease that primarily affects leaves, stems, and fruit, and can be infective from the seedling stage to the mature plant. Gray mold typically causes a 20-40% yield reduction, and in severe cases, over 60% in the field, significantly impacting agricultural production. For many years, benzimidazole, diformamide, or anilinopyrimidine fungicides have been used to control gray mold in eggplant. However, long-term use of these fungicides alone has led to increased pathogen resistance, significantly reducing the effectiveness of existing fungicides. Consequently, there is an urgent need to identify and develop new control agents.

[0004] Fenclofenone is a botanical fungicide with independent intellectual property rights in my country. Its chemical name is 1-o-hydroxyphenylbutanone, CAS: 2887-61-8, and its molecular formula is C 10 H 12 O2, the structural formula is as follows:

[0005]

[0006] Chinese patent application number CN99112525.8 discloses the use of the compound 1-o-hydroxyphenylbutanone, detailing its preparation process, structural formula, and physical properties. It also discloses that 1-o-hydroxyphenylbutanone has inhibitory and bactericidal effects against major diseases of vegetables, various crops, and fruit trees. However, the bactericidal effects of 1-o-hydroxyphenylbutanone vary when formulated with different compounds, necessitating further research. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a fungicide composition, fungicide and application for controlling eggplant gray mold, which has a bactericidal and synergistic effect on the pathogen of eggplant gray mold and can provide support for the screening and development of new control agents.

[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions.

[0009] A fungicide composition for preventing and controlling eggplant gray mold. The active ingredients of the fungicide composition are prepared by compounding fentrazone and mandipropamid, or fentrazone and zoxamide, or fentrazone and zinc pyrithione.

[0010] In the above-mentioned fungicidal composition for preventing and controlling eggplant gray mold, preferably, the mass ratio of fentrazone to mandipropamid is 1-10:20-1.

[0011] In the above-mentioned fungicidal composition for preventing and controlling eggplant gray mold, preferably, the mass ratio of fenoxamyl to zoxamidin is 1:15-1.

[0012] In the above-mentioned fungicidal composition for preventing and controlling gray mold of eggplant, preferably, the mass ratio of fenoxamyl to zoxamidin is 1:7.5.

[0013] In the above-mentioned fungicidal composition for preventing and controlling eggplant gray mold, preferably, the mass ratio of fentrazone to zinc pyrithione is 1-20:20-1.

[0014] As a general technical concept, the present invention also provides a fungicide comprising the above-mentioned fungicide composition.

[0015] As a general technical concept, the present invention also provides a use of the above-mentioned fungicide composition or the above-mentioned fungicide in preventing and controlling eggplant gray mold.

[0016] Compared with the prior art, the advantages of the present invention are:

[0017] (1) In the fungicidal composition of the present invention, when fenthion is compounded with dimethomorph, zoxamidin or zinc pyrithione, it exhibits a synergistic effect on inhibiting the growth of Botrytis cinerea hyphae within a certain mass ratio range. Compared with a single active ingredient, it can improve the inhibitory effect on pathogens, thereby improving the control effect on eggplant gray mold, and can provide support for the screening and development of new control agents.

[0018] (2) The fungicidal composition of the present invention can delay the development of resistance of the pathogen of eggplant gray mold and extend the service life of the agent.

[0019] (3) The bactericidal composition of the present invention can reduce the amount of active ingredients used, reduce the cost of prevention and control, and alleviate environmental pressure. DETAILED DESCRIPTION

[0020] The present invention will be further described below with reference to specific preferred embodiments, but the scope of protection of the present invention is not limited thereby. The materials and instruments used in the following examples are all commercially available.

[0021] Example 1

[0022] The invention discloses a fungicide composition for preventing and controlling eggplant gray mold. The active ingredients of the fungicide composition are prepared by compounding fenclorac and mandipropamid.

[0023] In this embodiment, the mass ratio of fentrazone to mandipropamid is 1-10:20-1.

[0024] A fungicide of the present invention includes the fungicide composition of the above embodiment.

[0025] The fungicide composition or fungicide of this embodiment can be used to prevent and control eggplant gray mold.

[0026] Example 2

[0027] The invention discloses a fungicide composition for preventing and treating eggplant gray mold. The active ingredients of the fungicide composition are prepared by compounding fenclorac and zoxamidin.

[0028] In this embodiment, the mass ratio of fenoxamyl to zoxamidin is 1:15-1, more preferably 1:7.5.

[0029] A fungicide of the present invention includes the fungicide composition of the above embodiment.

[0030] The fungicide composition or fungicide of this embodiment can be used to prevent and control eggplant gray mold.

[0031] Example 3

[0032] The invention discloses a fungicide composition for preventing and treating eggplant gray mold. The active ingredients of the fungicide composition are prepared by compounding fenclofenone and pyrithione zinc.

[0033] In this embodiment, the mass ratio of fentrazone to zinc pyrithione is 1-20:20-1.

[0034] A fungicide of the present invention includes the fungicide composition of the above embodiment.

[0035] The fungicide composition or fungicide of this embodiment can be used to prevent and control eggplant gray mold.

[0036] Indoor bioassay test

[0037] 1. Test strains: Infected eggplant leaves were collected and the pathogen of eggplant gray mold, Botrytis cinerea, was isolated in the laboratory and stored on PDA culture medium.

[0038] 2. Test agents

[0039] 98% fenoxam-1-one (Hubei Hengjingrui Chemical Co., Ltd.), 93% mandipropamid (Syngenta Crop Protection Co., Ltd., Switzerland), 97% zoxamidin (Dalian Kaifei Chemical Co., Ltd.), 99% zinc pyrithione (Shandong Haizhou Bioengineering Co., Ltd.).

[0040] Drug treatment: The test agent was first dissolved in dimethyl sulfoxide and then diluted with a 0.1% by mass Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions were set according to Examples 1-3. The specific proportions are shown in Tables 1-3. Five mass concentration gradients were set for each single agent and proportioned mixture according to the equal ratio method.

[0041] 3. Test methods

[0042] The hyphae growth rate method was used. 9 mL of pre-melted PDA medium was added to a sterile conical flask. 1 mL of drug solution was quantitatively pipetted sequentially, from low to high concentration, and added to each of the above conical flasks. After thorough shaking, the mixture was poured into a 9 cm diameter Petri dish to prepare drug-containing plates of the corresponding concentrations. A treatment without drug was used as a blank control, with five replicates for each treatment. A 5 mm diameter punch was used to cut a bacterial cake from the edge of the test strain colony. This cake was inoculated onto the center of the drug-containing plate and the blank control plate, with the hyphae facing upward. The plates were covered and incubated at 25°C. When the blank control colony diameter reached 2 / 3 of the Petri dish diameter, the colony diameter was measured using the cross-hatch method, and the hyphae growth inhibition rate of the different treatments was calculated.

[0043]

[0044] 4. Data analysis: DPS software was used for data statistical analysis. Linear regression was performed with the logarithmic value of fungicide concentration as x and the corresponding probability value of mycelial growth inhibition rate as y to obtain the toxicity regression equation and the toxicity EC of the agent to the target pathogen. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0045]

[0046] In the above formula: ATI - the toxicity index of the mixture; S - the EC of the standard agent 50 , the unit is mg / L; M--EC of the mixture 50 , unit is mg / L.

[0047] TTI=TI A ×P A +TI B ×P B

[0048] In the above formula: TTI - theoretical toxicity index of the mixture; TI A --Toxicity index of agent A; P A --The percentage of agent A in the mixture, expressed as percentage (%); TI B --Toxicity index of agent B; P B--The percentage of agent B in the mixture, in percentage (%).

[0049]

[0050] In the above formula: CTC - co-toxicity coefficient; ATI - measured toxicity index of the mixture; TTI - theoretical toxicity index of the mixture.

[0051] 5. Measurement results

[0052] The synergistic effect of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), with CTC ≤ 80 indicating antagonism, 80 < CTC < 120 indicating additive effect, and CTC ≥ 120 indicating synergism. The results are shown in Tables 1-3.

[0053] Table 1 Indoor bioactivity test of fenthiocarb and dimethomorph against Botrytis cinerea

[0054] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Fenclofenac 5.0568 100.0000 -- -- mandipropamid 41.8802 12.0744 -- -- Fenclofenac 1: Mandipropamid 60 39.7312 12.7275 13.5158 94.1675 Fenclofenac 1: Mandipropamid 30 30.9279 16.3503 14.9107 109.6544 Fenclofenac 1: Mandipropamid 20 24.2117 20.8858 16.2614 128.4379 Fenclofenac 1: Mandipropamid 10 16.4835 30.6780 20.0677 152.8725 Fenclofenac 1: Mandipropamid 5 10.5065 48.1302 26.7287 180.0694 Fenclofenac 1: Mandipropamid 1 6.6587 75.9428 56.0372 135.5220 Fenclofenac 5: Mandipropamid 1 2.0985 240.9721 85.3457 282.3482 Fenclofenac 10: Mandipropamid 1 1.5377 328.8548 92.0068 357.4246

[0055] As shown in Table 1, when fenoxam-1 and mandipropamid are compounded, when the mass ratio of fenoxam-1 to mandipropamid is 1:60-30, an additive effect is exhibited against Botrytis cinerea; when the mass ratio of fenoxam-1 to mandipropamid is 1-10:20-1, a synergistic effect is exhibited against Botrytis cinerea.

[0056] Table 2 Indoor bioactivity test of fenthiocarb and oxadiazine against Botrytis cinerea

[0057]

[0058]

[0059] As shown in Table 2, when fenoxamyl and zoxamide were combined, when the mass ratio of fenoxamyl and zoxamide was 5:1, an additive effect was shown on Botrytis cinerea; when the mass ratio of fenoxamyl and zoxamide was 1:15-1, a synergistic effect was shown on Botrytis cinerea, especially when the mass ratio was 1:7.5, the co-toxicity coefficient reached 653.1869, and the synergistic effect was particularly significant.

[0060] Table 3 Indoor bioactivity test of fenthion and zinc pyrithione against Botrytis cinerea

[0061] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Fenclofenac 5.0568 100.0000 -- -- Zinc pyrithione 8.3778 60.3595 -- -- Pyrithione Zinc 30 7.0011 72.2286 61.6382 117.1815 Pyrithione Zinc 20 5.1386 98.4081 62.2472 158.0925 Pyrithione Zinc 10 6.3740 79.3348 63.9632 124.0319 Pyrithione Zinc 5 3.8366 131.8042 66.9663 196.8218 Pyrithione Zinc 1 2.4069 210.0960 80.1798 262.0312 Pyrithione Zinc 1 3.3475 151.0620 93.3933 161.7483 Pyrithione Zinc 10: Pyrithione Zinc 1 3.7457 135.0028 96.3963 140.0497 Pyrithione Zinc 1 1.8154 278.5502 98.1124 283.9094 Fenclofenac 30: Zinc pyrithione 1 5.1562 98.0722 98.7213 99.3425

[0062] As shown in Table 3, when fencyclopyrim and pyrithione zinc are compounded, when the mass ratio of fencyclopyrim and pyrithione zinc is 1:30 and 30:1, an additive effect is shown on Botrytis cinerea; when the mass ratio of fencyclopyrim and pyrithione zinc is 1-20:20-1, a synergistic effect is shown on Botrytis cinerea.

[0063] In summary, when the fenoxamyl of the present invention is compounded with dimethomorph, zoxamidin or zinc pyrithione, it shows a synergistic effect in inhibiting the mycelial growth of Botrytis cinerea. Compared with a single active ingredient, it can improve the inhibitory effect on pathogens, thereby improving the control effect on eggplant gray mold, and can provide support for the screening and development of new control agents.

[0064] The above description is only a preferred embodiment of the present invention and does not constitute any formal limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the spirit and technical solution of the present invention, use the methods and technical contents disclosed above to make many possible changes and modifications to the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention, still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A fungicidal composition for preventing and treating eggplant gray mold, characterized in that: The active ingredient of the bactericidal composition is prepared by compounding fenoxam and zoxamidin, and the mass ratio of fenoxam to zoxamidin is 1:15-1.

2. The bactericidal composition for preventing and controlling eggplant gray mold according to claim 1, characterized in that The mass ratio of fenoxamyl to zoxamidoprop-3 is 1:7.

5.

3. A fungicide, characterized in that: The invention comprises the bactericidal composition according to claim 1 or 2.

4. Use of the fungicide composition according to claim 1 or 2 or the fungicide according to claim 3 in preventing and controlling eggplant gray mold.

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