Cosmetics with skin repair efficacy, their preparation methods, and compositions

Abstract

This application relates to the field of cosmetics, and relates to a cosmetic with skin repair efficacy, its preparation method, and composition. The cosmetic includes: 0.01 - 1% centella asiatica extract; 0.1 - 3% panthenol; 0.1 - 10% seawater; 0.001 - 5% self-assembled supramolecular polysaccharide; the balance is the matrix. The combination of each component achieves a synergistic effect; it can achieve excellent skin repair effects with fewer active ingredients, while reducing the viscosity of the cosmetic and improving the skin feel of the cosmetic. By combining centella asiatica extract, panthenol, seawater, and self-assembled supramolecular polysaccharide, the skin permeability of centella asiatica extract is greatly improved, the skin absorption effect is enhanced, and the addition amount of centella asiatica extract in the cosmetic is reduced. On the premise of a low addition amount, the cosmetic has excellent skin repair effects; it can simultaneously solve the problems of stickiness caused by a high addition amount of centella asiatica extract and poor skin feel of the cosmetic.

Description

Technical Field

[0001] This application relates to the field of cosmetics. Specifically, it relates to a cosmetic with skin repair efficacy, its preparation method, and composition. Background Art

[0002] Human skin is often affected by problems such as skin inflammation and damaged skin barrier caused by ultraviolet radiation, external stimuli, etc. When the skin barrier is damaged, it will lead to too fast loss of skin moisture and dry skin;

[0003] Skin inflammation caused by infection, irritants, or allergies can also damage the skin barrier.

[0004] Cosmetics play an important role in skin repair; there are various cosmetics for sensitive skin repair on the market. However, these repair cosmetics are uneven; there are fewer active ingredients that can be absorbed by the skin; they cannot achieve the real repair effect. Summary of the Invention

[0005] The purpose of the embodiments of this application is to provide a cosmetic with skin repair efficacy, its preparation method, and composition.

[0006] In the first aspect, this application provides a cosmetic with skin repair efficacy. By mass percentage, the cosmetic includes:

[0007] 0.01% - 1% centella asiatica extract;

[0008] 0.1% - 3% panthenol;

[0009] 0.1% - 10% seawater;

[0010] 0.001% - 5% self-assembled supramolecular polysaccharide; the balance is the matrix.

[0011] In the above technical solution, through the combination of centella asiatica extract, panthenol, seawater, and self-assembled supramolecular polysaccharide; the various components are compounded to achieve a synergistic effect; it can achieve excellent skin repair effects with fewer active ingredients, while reducing the greasiness of the cosmetic and improving the skin feel of the cosmetic.

[0012] Furthermore, centella asiatica extract, as a repair active ingredient, has skin repair efficacy. However, to achieve excellent skin repair efficacy, a high content (above 1 wt%, such as 1.5%, 2%) of centella asiatica extract needs to be added. However, the currently commercially available centella asiatica extract has a sticky property, which will greatly increase the greasiness of the cosmetic when added to the cosmetic; this will greatly reduce the skin feel of the cosmetic and the consumer experience, restricting its application; and the addition amount will also increase the cost, further restricting its application.

[0013] In the above technical solution of the present application, by compounding centella asiatica extract, panthenol, seawater, and self-assembled supramolecular polysaccharide, the skin permeability of centella asiatica extract can be greatly improved, and the skin absorption effect can be enhanced. As a result, the addition amount of centella asiatica extract in cosmetics can be reduced, and on the premise of a relatively low addition amount, the cosmetics can still have excellent skin repair effects. Thus, the problems of greasiness and poor skin feel caused by a high addition amount of centella asiatica extract can be solved simultaneously.

[0014] In other embodiments of the present application, the cosmetics include, by mass percentage:

[0015] 0.1% - 1% centella asiatica extract;

[0016] 0.3% - 3% panthenol;

[0017] 0.5% - 10% seawater;

[0018] 0.5% - 5% self-assembled supramolecular polysaccharide; the balance is the matrix.

[0019] In other embodiments of the present application, the cosmetics include, by mass percentage:

[0020] 0.1% - 0.6% centella asiatica extract;

[0021] 0.5% - 2% panthenol;

[0022] 3% - 10% seawater;

[0023] 1% - 4% self-assembled supramolecular polysaccharide; the balance is the matrix.

[0024] In other embodiments of the present application, the centella asiatica extract includes: asiaticoside, madecassoside, cyclodextrin; the cyclodextrin is coated on the surfaces of asiaticoside and madecassoside.

[0025] In other embodiments of the present application, by weight parts, the self-assembled supramolecular polysaccharide includes: dendrobium officinale polysaccharide and sodium hyaluronate; the dendrobium officinale polysaccharide and sodium hyaluronate self-assemble into supramolecular polysaccharide.

[0026] In other embodiments of the present application, the matrix includes: thickening agent, humectant, chelating agent, pH neutralizer.

[0027] In other embodiments of the present application, the thickening agent includes at least one of carbomer, xanthan gum, hydroxyethyl cellulose, or acrylic acid (ester) class.

[0028] In other embodiments of the present application, the humectant includes at least one of butylene glycol, glycerol, 1,3-butylene glycol, or pentylene glycol.

[0029] In other embodiments of the present application, the chelating agent includes at least one of sodium edetate, ethylenediaminetetraacetic acid, tetrasodium ethylenediaminetetraacetate, or disodium nitrilotriacetate.

[0030] In other embodiments of the present application, the pH neutralizing agent includes at least one of citric acid, lactic acid, potassium hydroxide, sodium hydroxide, disodium hydrogen phosphate, or triethanolamine.

[0031] In other embodiments of the present application, by mass percentage, the cosmetic includes:

[0032] 0.01% to 1% of centella asiatica extract;

[0033] 0.1% to 3% of panthenol;

[0034] 0.1% to 10% of seawater;

[0035] 0.001% to 5% of self-assembled supramolecular polysaccharide;

[0036] 0.1% to 0.5% of thickening agent;

[0037] 1% to 5% of humectant;

[0038] 0.01% to 1% of chelating agent;

[0039] 0.01% to 0.1% of pH neutralizing agent; the balance is water.

[0040] In a second aspect, the present application provides a method for preparing a cosmetic having a skin repair effect, including:

[0041] Mix the thickening agent, humectant, chelating agent with water, dissolve at 80°C to 90°C, and disperse evenly to obtain a first mixed material;

[0042] Cool the first mixed material to 40°C to 50°C, add centella asiatica extract, panthenol, seawater, self-assembled supramolecular polysaccharide, pH neutralizing agent, and stir to mix evenly.

[0043] In a third aspect, the present application provides a composition having a skin repair effect. By mass fraction, the composition includes:

[0044] 0.01% to 1% of centella asiatica extract;

[0045] 0.1% to 3% of panthenol;

[0046] 0.1% to 10% of seawater;

[0047] 0.001% to 5% of self-assembled supramolecular polysaccharide. Description of the Drawings

[0048] To more clearly illustrate the technical solutions of the embodiments of the present application, the accompanying drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application and should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0049] Figure 1 Graphs of cell scratch test data for each example and comparative example;

[0050] Figure 2 Graph of cells at 0 h in the blank control of the cell scratch test;

[0051] Figure 3 Graph of cells at 24 h in the blank control of the cell scratch test;

[0052] Figure 4 Graph of cells at 0 h in the positive control of the cell scratch test;

[0053] Figure 5 Graph of cells at 24 h in the positive control of the cell scratch test;

[0054] Figure 6 Graph of cells at 0 h in Example 2 of the cell scratch test;

[0055] Figure 7 Graph of cells at 24 h in Example 2 of the cell scratch test;

[0056] Figure 8 Graph of cells at 0 h in Comparative Example 2 of the cell scratch test;

[0057] Figure 9 Graph of cells at 24 h in Comparative Example 2 of the cell scratch test. Detailed implementation manners

[0058] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present application.

[0059] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application to be protected, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0060] The embodiments of the present application provide a cosmetic with skin repair efficacy. In terms of mass percentage, the cosmetic includes:

[0061] 0.01% - 1% Centella asiatica extract;

[0062] 0.1% - 3% Panthenol;

[0063] 0.1% - 10% Seawater;

[0064] 0.001% - 5% Self - assembled supramolecular polysaccharide; the balance is the matrix.

[0065] In the above technical solution, through the compounding of Centella asiatica extract, panthenol, seawater, and self - assembled supramolecular polysaccharide; the synergistic effect is achieved; it can achieve excellent skin repair effects with fewer active ingredients, while reducing the greasiness of the cosmetic and improving the skin feel of the cosmetic.

[0066] Exemplarily, in some embodiments of the present application, by mass percentage, the cosmetic includes:

[0067] Centella asiatica extract with a content of 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or a range between any two of the foregoing values;

[0068] Panthenol with a content of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 3% or a range between any two of the foregoing values;

[0069] Seawater with a content of 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10% or a range between any two of the foregoing values;

[0070] Self - assembled supramolecular polysaccharide with a content of 0.001%, 0.002%, 0.005%, 0.008%, 0.1%, 0.2%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or a range between any two of the foregoing values; the balance is the matrix.

[0071] Further optionally, in some embodiments of the present application, by mass percentage, the cosmetic includes:

[0072] 0.1% - 1% Centella asiatica extract;

[0073] 0.3% - 3% Panthenol;

[0074] 0.5% to 10% seawater;

[0075] 0.5% to 5% self - assembled supramolecular polysaccharide; the balance is the matrix.

[0076] Further optionally, in some embodiments of the present application, by mass percentage, the cosmetic comprises:

[0077] 0.1% to 0.6% centella asiatica extract;

[0078] 0.5% to 2% panthenol;

[0079] 3% to 10% seawater;

[0080] 1% to 4% self - assembled supramolecular polysaccharide; the balance is the matrix.

[0081] The main active ingredients of centella asiatica extract that take effect are asiaticoside and madecassoside. The solubility of these two substances is poor, which limits their application in cosmetic formulations. Although cyclodextrin - based encapsulation has been used to solve the water - solubility problem, when pursuing high repair effects, the addition amount is large, and the stickiness is obvious, greatly reducing the consumer experience, and the price is high. Panthenol, as a B - group vitamin, can be converted into pantothenic acid in the body, can promote normal keratinization of the skin, promote wound healing, etc. Panthenol has excellent water - solubility, but its permeability is poor, and the skin feel is very sticky at high concentrations. It can not only increase the repair effect of the product, but also reduce the usage amount and improve the skin feel. At the same time, adding seawater, which is rich in various minerals, the high permeability of natural and balanced minerals and trace elements brings a cool feeling to the skin while enhancing the bioavailability of other active molecules. The self - assembled supramolecular polysaccharide used can form an ordered network film on the skin. While moisturizing, it can inhibit water evaporation. These four substances can enhance the skin barrier effect through mutual interaction.

[0082] Furthermore, the cosmetic provided by the present application has good repair and moisturizing effects, and the experience is improved.

[0083] Furthermore, the cosmetic provided by the present application has high cost - effectiveness; it can greatly reduce the content of centella asiatica extract (this ingredient has a high cost and has problems such as stickiness and easy to cause poor skin feel); it can achieve excellent repair and moisturizing effects under the premise of a low content, and at the same time has a better skin feel, greatly reducing the stickiness.

[0084] Further, in some embodiments of the present application, the centella asiatica extract comprises: asiaticoside, madecassoside, cyclodextrin - based; the cyclodextrin - based is encapsulated on the surface of asiaticoside and madecassoside.

[0085] Due to the poor solubility of asiaticoside and madecassoside, the application of these two substances in cosmetic formulations is limited. By using cyclodextrin-based encapsulation treatment, the water solubility problem can be solved; by reducing their addition amount in cosmetics, the viscosity can be reduced and the skin feel can be improved.

[0086] Further optionally, in some embodiments of the present application, the above-mentioned centella extract (asiaticoside and madecassoside encapsulated with cyclodextrin) can be obtained by purchasing commercially.

[0087] Exemplarily, in some alternative embodiments of the present application, the above-mentioned centella extract can be prepared by the following method:

[0088] Dissolve β-cyclodextrin in water and add ethanol;

[0089] Add asiaticoside and madecassoside, stir and filter;

[0090] Take the filtrate; after removing ethanol from the filtrate, dry to obtain the centella extract encapsulated with cyclodextrin.

[0091] Further exemplarily, in some embodiments of the present application,

[0092] the above-mentioned centella extract can be prepared by the following method:

[0093] Dissolve β-cyclodextrin in water and add ethanol;

[0094] Add the water extract of centella (rhizome, stem and leaf), stir and filter;

[0095] Take the filtrate; after removing ethanol from the filtrate, dry to obtain the centella extract encapsulated with cyclodextrin.

[0096] Further, in some embodiments of the present application, by weight, the self-assembled supramolecular polysaccharide includes: dendrobium officinale polysaccharide and sodium hyaluronate; the dendrobium officinale polysaccharide and sodium hyaluronate self-assemble into a supramolecular polysaccharide.

[0097] The inventors found that skin moisture loss will seriously affect the repair effect of cosmetics on the skin. The cosmetics of the present application can form an ordered, network-like film on the skin by compounding the self-assembled supramolecular polysaccharide. While moisturizing, it can inhibit water evaporation, greatly improving the repair effect of the above-mentioned cosmetics. Through interaction with other cosmetic ingredients, synergistic effects can be achieved, enhancing the skin barrier effect; and there is a certain sustained-release effect on the release of active ingredients; further facilitating the absorption of active ingredients by the skin, thus further facilitating the improvement of the repair effect of cosmetics on the skin.

[0098] Further, in some embodiments of the present application, the above self-assembled supramolecular polysaccharide can be obtained by purchasing commercially.

[0099] Exemplarily, in some embodiments of the present application, the above self-assembled supramolecular polysaccharide can be prepared by the following method:

[0100] Extract the rhizome of Dendrobium officinale by the water extraction method; obtain the water extract of the rhizome of Dendrobium officinale;

[0101] Mix the water extract of the rhizome of Dendrobium officinale with glycerol to obtain a mixed solution, add 1,2-pentanediol and 1,2-hexanediol to the mixed solution, stir at 80°C to 100°C for 30 min to 60 min, and then cool.

[0102] The above Dendrobium polysaccharide and sodium hyaluronate self-assemble into a supramolecular polysaccharide, which can further improve the moisturizing effect; when compounded in the cosmetics of the present application, it can play a synergistic effect with skin repair active substances; and further improve the skin repair effect.

[0103] Further, in some embodiments of the present application, the thickener includes at least one of carbomer, xanthan gum, hydroxyethyl cellulose or acrylic acid (ester) class.

[0104] Exemplarily, in some embodiments of the present application, the above thickener includes any one of carbomer, xanthan gum, hydroxyethyl cellulose or acrylic acid (ester) class; or in some embodiments of the present application, the above thickener is a mixture of carbomer and xanthan gum; or in some embodiments of the present application, the above thickener is a mixture of carbomer, xanthan gum and hydroxyethyl cellulose; or in some embodiments of the present application, the above thickener is a mixture of carbomer, xanthan gum and hydroxyethyl cellulose; or in some embodiments of the present application, the above thickener is a mixture of carbomer and acrylic acid (ester) class; each raw material in each of the above mixtures can be mixed in any proportion.

[0105] Further, in some embodiments of the present application, the moisturizer includes at least one of butanediol, glycerol, 1,3-butanediol or pentanediol.

[0106] Exemplarily, in some embodiments of the present application, the humectant includes any one of butylene glycol, glycerin, 1,3 - butylene glycol, or pentylene glycol. Or in some embodiments of the present application, the humectant is a mixture of butylene glycol, glycerin, 1,3 - butylene glycol, and pentylene glycol. Or in some embodiments of the present application, the humectant is a mixture of butylene glycol and glycerin; or in some embodiments of the present application, the humectant is a mixture of 1,3 - butylene glycol and pentylene glycol; or in some embodiments of the present application, the humectant is a mixture of butylene glycol and pentylene glycol. Each raw material in the above mixtures can be mixed in any proportion.

[0107] Further, in some embodiments of the present application, the chelating agent includes at least one of disodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid, tetrasodium ethylenediaminetetraacetate, or disodium nitrilotriacetate.

[0108] Exemplarily, in some embodiments of the present application, the chelating agent is any one of disodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid, tetrasodium ethylenediaminetetraacetate, or disodium nitrilotriacetate; or in some embodiments of the present application, the chelating agent is a mixture of disodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid, and tetrasodium ethylenediaminetetraacetate; or in some embodiments of the present application, the chelating agent is a mixture of tetrasodium ethylenediaminetetraacetate and disodium nitrilotriacetate. Each raw material in the above mixtures can be mixed in any proportion.

[0109] Further, in some embodiments of the present application, the pH neutralizing agent includes at least one of organic acids and bases, inorganic acids and bases; further optionally, the pH neutralizing agent includes at least one of aminomethylpropanol, citric acid, lactic acid, potassium hydroxide, sodium hydroxide, disodium hydrogen phosphate, or triethanolamine.

[0110] Exemplarily, in some embodiments of the present application, the pH neutralizing agent includes any one of aminomethylpropanol, citric acid, lactic acid, potassium hydroxide, sodium hydroxide, disodium hydrogen phosphate, or triethanolamine; or in some embodiments of the present application, the pH neutralizing agent is a mixture of aminomethylpropanol, citric acid, lactic acid, potassium hydroxide, and sodium hydroxide; or in some embodiments of the present application, the pH neutralizing agent is a mixture of aminomethylpropanol, sodium hydroxide, and disodium hydrogen phosphate. Each raw material in the above mixtures can be mixed in any proportion.

[0111] Further, in some embodiments of the present application, by mass percentage, the cosmetic includes:

[0112] 0.01% - 1% centella asiatica extract;

[0113] 0.1% - 3% panthenol;

[0114] 0.1% to 10% seawater;

[0115] 0.001% to 5% self - assembled supramolecular polysaccharide;

[0116] 0.1% to 0.5% thickener;

[0117] 1% to 5% humectant;

[0118] 0.01% to 1% chelating agent;

[0119] 0.01% to 0.1% pH neutralizer; the balance is water.

[0120] Exemplarily, in some embodiments of the present application, by mass percentage, the cosmetic composition comprises:

[0121] Centella asiatica extract in an amount of 0.01%, 0.02%, 0.03%, 0.05%, 0.08%, 0.1%, 0.2%, 0.3%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or in a range between any two of the foregoing values;

[0122] Panthenol in an amount of 0.1%, 0.2%, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.6%, 3% or in a range between any two of the foregoing values;

[0123] Seawater in an amount of 0.1%, 0.2%, 0.5%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 3%, 3.2%, 3.5%, 4%, 5%, 6%, 7%, 8%, 9%, 10% or in a range between any two of the foregoing values;

[0124] Self - assembled supramolecular polysaccharide in an amount of 0.001%, 0.005%, 0.008%, 0.1%, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 2%, 3%, 3.5%, 4%, 4.5%, 5% or in a range between any two of the foregoing values;

[0125] Thickener in an amount of 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5% or in a range between any two of the foregoing values;

[0126] Humectant in an amount of 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or in a range between any two of the foregoing values;

[0127] Chelating agents in an amount of 0.01%, 0.02%, 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or in a range between any two of the foregoing values;

[0128] pH neutralizing agents in an amount of 0.01%, 0.02%, 0.05%, 0.08%, 0.09%, 0.1% or in a range between any two of the foregoing values; the balance is water.

[0129] Some embodiments of the present application provide a method for preparing a cosmetic having a skin repair effect, including:

[0130] Mix a thickening agent, a humectant, a chelating agent and water, dissolve at 80°C to 90°C, and disperse evenly to obtain a first mixed material;

[0131] Cool the first mixed material to 40°C to 50°C, add centella asiatica extract, panthenol, seawater, self-assembled supramolecular polysaccharide, and a pH neutralizing agent, and stir and mix evenly.

[0132] According to the above technical solution, by adopting the above preparation method, a cosmetic having a skin repair effect can be prepared.

[0133] Further exemplarily, in some embodiments of the present application, mix a thickening agent, a humectant, a chelating agent and water, dissolve at a temperature of 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, 86°C, 87°C, 88°C, 89°C, 90°C or in a range between any two of the foregoing values, and disperse evenly to obtain a first mixed material;

[0134] Cool the first mixed material to 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C, 50°C or in a range between any two of the foregoing values, add centella asiatica extract, panthenol, seawater, self-assembled supramolecular polysaccharide, and a pH neutralizing agent, and stir and mix evenly.

[0135] Some embodiments of the present application provide a composition having a skin repair effect. In terms of mass parts, the composition includes:

[0136] 0.01% - 1% centella asiatica extract; 0.1% - 3% panthenol; 0.1% - 10% seawater; 0.001% - 5% self-assembled supramolecular polysaccharide.

[0137] In other alternative embodiments of the present application, preservatives may be optionally added to the above-mentioned cosmetics and compositions. Exemplarily, in some embodiments of the present application, the above-mentioned preservatives may include Naturcare HL-10plus, phenoxyethanol, etc.

[0138] The features and properties of the present application will be further described in detail with reference to the following examples:

[0139] Examples, Comparative Examples

[0140] A cosmetic with skin repair efficacy is provided. The ingredients of each example are shown in Table 1 by mass percentage.

[0141] Table 1

[0142]

[0143]

[0144] Production steps:

[0145] 1. Mix the raw materials in Group A, then stir and heat to 85 °C, and disperse evenly;

[0146] 2. Cool down to 45 °C, add the raw materials in Group B, and stir evenly.

[0147] Test items and conclusions

[0148] 1. Test item: In vitro repair - cell scratch

[0149] Preparation of test samples

[0150] Sample group: Dilute the cosmetic samples prepared in each example and comparative example into 5% concentration solutions with a medium containing 2% serum.

[0151] Blank control: Medium containing 2% serum.

[0152] Positive control: Medium containing 10% serum.

[0153] Efficacy evaluation method and evaluation

[0154] Place the wound healing four-well insert into a 12-well plate, inoculate log-phase BJ cells into the 12-well plate, add 110 μL of cell suspension to each well in the four-well insert, and the inoculation density is 1 * 105 cells per well. Place it in a carbon dioxide incubator at 37 °C and culture for 24 h. After the cells are basically confluent, gently remove the wound healing four-well insert, then wash it once with PBS, add 110 μL of 2% serum medium containing the sample to each well in the four-well insert, and place it in a carbon dioxide incubator at 37 °C and culture until the observation time set in the experiment. At the set time, observe and measure the width of the scratch with a microscope and take pictures.

[0155] The test results can be found in Appendix Figure 1 ~Appendix Figure 9 of the specification.

[0156] Conclusion:

[0157] From the results in Appendix Figure 1 ~Appendix Figure 9 of the specification, it can be seen that the cell scratch repair effect of each example is significantly better than that of each comparative example.

[0158] Furthermore, by comparing each example with the positive control group, it can be seen that the cell scratch repair effect of each example is almost close to that of the positive control group. Thus, it can be shown that the cosmetics provided by the examples of the present application have very excellent cell scratch repair effects.

[0159] Furthermore, by comparing each example with Comparative Example 4, it can be seen that when the "centella asiatica extract, panthenol, seawater, self-assembled supramolecular polysaccharide" of the present application is not used in a synergistic combination, it is necessary to greatly increase the addition amount of the "centella asiatica extract" in order to improve it to the level of Example 1 of the present application (the addition amount of Comparative Example 4 is 15 times that of Example 1); the repair effect. And such a high dosage will inevitably greatly increase the viscosity of the cosmetics; reduce the feel of the cosmetics, restricting its scope of application. Moreover, such a large dosage will also result in too high a cost, further restricting its scope of application.

[0160] 2. Test item: Sensory evaluation

[0161] Select 10 evaluators to evaluate the product usage experience

[0162] Evaluation process:

[0163] ① Before use, observe indicators such as gloss through the container;

[0164] ② During use, first take a 4*4 cm area on the inner side of the forearm as the test area, use a syringe to take 0.05 mL of the sample to the area to be tested, disperse the sample with the index finger, gently stroke it, and slightly make circles at a frequency of 1 circle / s. After smearing about 15 circles, compare with the reference sample, and evaluate and score each indicator from 0 to 10 according to the requirements and record;

[0165] ③ After the product is completely absorbed for 5 minutes, evaluate and score each indicator from 0 to 10 according to the requirements and record.

[0166] Evaluation dimensions: Appearance before use. Degree of heaviness, moisturizing feeling, smooth feeling, absorbency, spreadability, stickiness, soft feeling, smooth feeling, and residual feeling during use are scored for overall preference.

[0167] The test results are shown in the following table:

[0168] Table 2

[0169]

[0170] Conclusion:

[0171] From the above test results, the scores of each embodiment are all above 8 points. It can be seen that the cosmetics of the embodiments of the present application have excellent skin feel, lower greasiness, and are more suitable for daily use as repair cosmetics.

[0172] Furthermore, by comparing Example 1 with Comparative Example 4, it can be seen that after adding 15% of "Centella asiatica extract" to Comparative Example 4, its skin feel is greatly reduced, further proving that its greasiness is too large, which will limit its use as daily-use cosmetics.

[0173] 3. Test item: Repair efficacy test

[0174] Thirty-two healthy volunteers were selected. For half-face control test, after cleansing the face at night, take out the mask and stick it tightly on the facial skin, and then gently stroke it with fingertips to make it fit the facial contour; after 10 - 15 minutes, remove the mask and gently massage the essence until it is absorbed. The test period is 7 days (once every other day).

[0175] Test content: Transdermal water loss of the skin, water content of the skin stratum corneum, self-assessment questionnaire, adverse reaction assessment.

[0176] The test results are shown in the following table:

[0177] Table 3

[0178]

[0179]

[0180] Conclusion: It can be seen from the above test results that:

[0181] Compared with Comparative Example 4, the transdermal water loss of the skin in Example 1 of the present application is reduced by a larger margin, and the water content of the stratum corneum is also increased more, indicating that the moisturizing and repair effect of the example is better than that of the comparative example.

[0182] Since Example 1 of the present application has the lowest cell scratch repair effect; thus, it can be confirmed without doubt that other embodiments of the present application must have better repair and moisturizing effects relative to the example.

[0183] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various modifications and variations can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. A cosmetic with skin repairing effect, characterized in that: In terms of mass percentage, the cosmetic comprises: 0.1%~0.6% Centella asiatica extract; 0.5%~2% panthenol; 3%~10% sea water; 1%~4% self-assembled supramolecular polysaccharide; the rest is matrix; The above self-assembling supramolecular polysaccharide is prepared according to the following method: Extracting the rhizome of Dendrobium officinale by water extraction method; obtaining the water extract of the rhizome of Dendrobium officinale; The water extract of the rhizome of Dendrobium officinale is mixed with glycerol to obtain a mixed solution, 1,2-pentanediol and 1,2-hexanediol are added to the mixed solution, and the mixture is stirred at 80°C to 100°C for 30min to 60min, and then cooled; Dendrobium polysaccharide and sodium hyaluronate are self-assembled into supramolecular polysaccharide.

2. The cosmetic with skin repairing effect according to claim 1, characterized in that: The Centella asiatica extract comprises: asiatica glycoside, madecassoside and cyclodextrins; the cyclodextrins are coated on the surface of the asiatica glycoside and the madecassoside.

3. The cosmetic with skin repairing effect according to claim 1, characterized in that: The self-assembling supramolecular polysaccharide comprises: dendrobium polysaccharide and sodium hyaluronate; the dendrobium polysaccharide and the sodium hyaluronate are self-assembled into supramolecular polysaccharide.

4. The cosmetic with skin repairing effect according to any one of claims 1 to 3, characterized in that: The matrix comprises: a thickener, a moisturizer, a chelating agent, and a pH neutralizer.

5. The cosmetic with skin repairing effect according to claim 4, characterized in that: The thickener comprises at least one of carbomer, xanthan gum, hydroxyethyl cellulose or acrylates.

6. The cosmetic with skin repairing effect according to claim 4, characterized in that: The moisturizing agent includes at least one of butylene glycol, glycerol or pentylene glycol.

7. The cosmetic with skin repairing effect according to claim 4, characterized in that: The chelating agent includes at least one of disodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid or tetrasodium ethylenediaminetetraacetate.

8. The cosmetic with skin repairing effect according to claim 4, characterized in that: The pH neutralizer includes at least one of citric acid, lactic acid, potassium hydroxide, sodium hydroxide, disodium hydrogen phosphate or triethanolamine.

9. The cosmetic with skin repairing effect according to claim 4, characterized in that: In terms of mass percentage, the cosmetic comprises: 0.1%~0.6% Centella asiatica extract; 0.5%~2% panthenol; 3%~10% sea water; 1%~4% self-assembled supramolecular polysaccharide; 0.1%~0.5% thickener; 1%~5% moisturizer; 0.01%~1% chelating agent; 0.01%~0.1% pH neutralizer; the balance is water.

10. The method for preparing the cosmetics with skin repairing effect according to any one of claims 4 to 9, characterized in that: include: Mixing a thickener, a moisturizer, a chelating agent and water, dissolving at 80° C. to 90° C., and dispersing uniformly to obtain a first mixed material; The first mixed material is cooled to 40° C. to 50° C., and Centella asiatica extract, panthenol, seawater, self-assembled supramolecular polysaccharide, and pH neutralizer are added, and the mixture is stirred and mixed evenly.

11. A composition having skin repairing effect, characterized in that: In terms of mass percentage, the composition comprises: 0.1%~0.6% Centella asiatica extract; 0.5%~2% panthenol; 3%~10% sea water; 1%~4% self-assembled supramolecular polysaccharide; The above self-assembling supramolecular polysaccharide is prepared according to the following method: Extracting the rhizome of Dendrobium officinale by water extraction method; obtaining the water extract of the rhizome of Dendrobium officinale; The water extract of the rhizome of Dendrobium officinale is mixed with glycerol to obtain a mixed solution, 1,2-pentanediol and 1,2-hexanediol are added to the mixed solution, and the mixture is stirred at 80°C to 100°C for 30min to 60min, and then cooled; Dendrobium polysaccharide and sodium hyaluronate are self-assembled into supramolecular polysaccharide.

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