Traditional Chinese medicine foot soaking bag with effects of warming meridians, dispelling cold, and removing wind and dampness and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine foot-soaking bag with the effects of warming meridians, dispelling cold, and removing wind and dampness, and a preparation method thereof, and relates to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine foot-soaking bag is prepared from the following traditional Chinese medicines in parts by weight: 20-40 parts of wormwood leaf, 10-30 parts of aconite root, 10-30 parts of notopterygium root, 5-20 parts of white tribulus terrestris, 5-20 parts of clematis root, 1-10 parts of acanthopanax bark, 1-10 parts of leucoderma corylifolia, 1-10 parts of cynanchum wilfordii, and 1-10 parts of asarum. The traditional Chinese medicine foot-soaking bag extracts effective components of the traditional Chinese medicine through supercritical fluid extraction and solvent extraction, adopts the extract and residue to prepare the foot-soaking bag, and combines hot water soaking of the foot-soaking bag during use to achieve full utilization of water-soluble effective components and oil-soluble effective components contained in the traditional Chinese medicine, improve the utilization rate of effective components of the traditional Chinese medicine, increase the content of effective components in the foot-soaking bag, and thus improve the use effect of the foot-soaking bag.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, and in particular to a traditional Chinese medicine foot-soaking bag with the effects of warming meridians, dispelling cold, and removing wind and dampness, and a preparation method thereof. Background Art

[0002] Foot soaking is a type of foot bath, one of the contents of traditional Chinese medicine foot therapy, and also a commonly used external treatment method. The foot is also called the second heart of the human body. Studies have shown that there are reflex areas and meridian distributions corresponding to various internal organs on the human feet. When soaking feet in warm water, these reflex areas can be stimulated, promoting blood circulation in the human body, regulating the endocrine system, enhancing the function of human organs, and achieving health care effects of disease prevention and treatment. At the same time, the warm stimulation of the peripheral nerves of the feet when soaking feet in hot water can inhibit the cerebral cortex, making people feel comfortable and relaxed in the brain, thereby accelerating sleep and deepening sleep.

[0003] At present, there are many foot soaking bags with health care effects on the market. Although they are added with multiple Chinese medicines, their effects are poor and it is difficult to achieve effective therapeutic effects. And people know that different Chinese medicine prescriptions have different medicinal effects. If you want to have a good relief or even cure effect on a certain symptom in a targeted manner, you must choose a specific Chinese medicine prescription. In addition, the existing foot soaking bags are generally directly made of multiple Chinese medicines, and the effective ingredients of some Chinese medicines are relatively slow to leaching in water or water is not a good solvent, resulting in these effective ingredients cannot be fully utilized, thereby causing a waste of Chinese medicine resources. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a Chinese medicine foot bath bag with the effects of warming the meridians and dispelling cold and dispelling wind and dampness and a preparation method thereof, which greatly improves the utilization rate of Chinese medicine, enables the effective ingredients of Chinese medicine to fully exert their effects, shortens the onset time, and thus quickly relieves symptoms.

[0005] The technical problem to be solved by the present invention is achieved by adopting the following technical solutions:

[0006] The first object of the present invention is to provide a Chinese medicine foot bath bag with the effects of warming the meridians, dispelling cold, and dispelling wind and dampness, which is made of the following Chinese medicines in parts by weight:

[0007]

[0008] The present invention adds Tribulus terrestris and Leprosy on the basis of seven traditional Chinese medicines (Artemisia argyi, Chuanwu, Notopterygium wilfordii, Clematidis, Acanthopanax acanthopanax, Cynanchum indica, Asarum) known in the art that have the effects of warming the meridians and dispelling cold and dispelling wind and dampness. The known effects of Tribulus terrestris are calming the liver and relieving depression, dispelling wind and improving eyesight, and the known effects of Leprosy are clearing away heat and detoxifying, promoting blood circulation and dispersing knots. It is found through experiments that the appropriate addition of these two traditional Chinese medicines can improve the use effect of the foot soaking bag. However, the known effects of Tribulus terrestris and Leprosy do not provide technical inspiration for using these two traditional Chinese medicines to prepare a traditional Chinese medicine foot soaking bag with the effects of warming the meridians and dispelling cold and dispelling wind and dampness. The inventor obtained the formula of the present invention by screening hundreds of formulas, and the addition of Tribulus terrestris or Leprosy alone will not substantially change the use effect of the foot soaking bag.

[0009] The second object of the present invention is to provide a method for preparing the Chinese medicine foot soaking bag having the effects of warming meridians, dispelling cold, and dispelling wind and dampness, comprising the following steps:

[0010] (1) crushing wormwood leaf, chuanwu root, notopterygium root, tribulus terrestris, clematis root, acanthopanax bark, leucophylla scabra, cynanchum chinense and asarum to extract volatile oil by supercritical fluid extraction to obtain volatile oil and residue I;

[0011] (2) extracting the residue I with an ethanol solution by refluxing, and separating the solid from the liquid to obtain an extract and a residue II;

[0012] (3) drying and crushing the residue II to obtain powder I;

[0013] (4) concentrating the extract under reduced pressure, separating it by column chromatography using a macroporous adsorption resin, and collecting the eluate;

[0014] (5) concentrating the eluate under reduced pressure, drying it, and crushing it to obtain powder II;

[0015] (6) The volatile oil, powder I and powder II are fully mixed and then packaged with non-woven fabric to obtain a traditional Chinese medicine foot bathing bag.

[0016] The beneficial effects of the present invention are as follows: the present invention extracts effective ingredients of traditional Chinese medicine by supercritical fluid extraction and solvent extraction, uses the extract and the residue to prepare a foot soaking bag, and combines the hot water soaking of the foot soaking bag during use to achieve full utilization of water-soluble effective ingredients and oil-soluble effective ingredients contained in the traditional Chinese medicine, improve the utilization rate of the effective ingredients of the traditional Chinese medicine, increase the content of the effective ingredients in the foot soaking bag, and thus improve the use effect of the foot soaking bag. DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments.

[0018] The present invention provides a traditional Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness, which is made of the following traditional Chinese medicines in parts by weight:

[0019]

[0020] The present invention also provides a method for preparing the Chinese medicine foot soaking bag having the effects of warming meridians, dispelling cold, and dispelling wind and dampness, comprising the following steps:

[0021] (1) crushing wormwood leaf, chuanwu, notopterygium root, tribulus terrestris, clematis root, acanthopanax bark, leucophylla scabra, cynanchum chinense and asarum, and extracting volatile oil by supercritical fluid extraction to obtain volatile oil and residue I;

[0022] (2) extracting the residue I with an ethanol solution by refluxing, and separating the solid from the liquid to obtain an extract and a residue II;

[0023] (3) drying and crushing the residue II to obtain powder I;

[0024] (4) concentrating the extract under reduced pressure, performing column chromatography separation using a macroporous adsorption resin, and collecting the eluate;

[0025] (5) concentrating the eluate under reduced pressure, drying it, and crushing it to obtain powder II;

[0026] (6) The volatile oil, powder I and powder II are fully mixed and then packaged with non-woven fabric to obtain a traditional Chinese medicine foot bathing bag.

[0027] Furthermore, the supercritical fluid extraction method uses CO2 as an extractant, the extraction pressure is 10-30MPa, the extraction temperature is 30-50°C, and the extraction time is 0.5-5h. The volatile oil of traditional Chinese medicine is extracted by supercritical fluid extraction to achieve efficient utilization of volatile oil. Supercritical fluid extraction has the advantages of high efficiency, environmental protection, safety, energy saving and good product quality.

[0028] Furthermore, the volume fraction of the ethanol solution is 60-100%. Ethanol or ethanol water solution is used to extract the effective components of traditional Chinese medicine, and the ethanol is easy to remove and can be reused after recovery, thereby reducing costs.

[0029] Furthermore, the solid-liquid ratio of the reflux extraction is 1 g: (20-100) mL, the extraction time for each extraction is 0.5 to 2 hours, and the number of extractions is 1 to 3 times.

[0030] Furthermore, the solid-liquid separation is filtration or centrifugation. Filtration or centrifugation can be used for solid-liquid separation.

[0031] Furthermore, the temperature of the reduced pressure concentration is 55-65° C. The organic solvent is recovered by reduced pressure concentration, thereby avoiding the waste of the organic solvent, achieving the reuse of the organic solvent, reducing costs, and reducing environmental pollution.

[0032] Furthermore, the column chromatography separation uses an ethanol solution with a volume concentration of 20-60% for gradient elution. Column chromatography separation has the advantages of mild separation conditions, high stability, good repeatability, and high product recovery rate.

[0033] Furthermore, the macroporous adsorption resin is one of D101 macroporous adsorption resin, AB-8 macroporous adsorption resin, and DM130 macroporous adsorption resin. Macroporous adsorption resin is a highly porous organic polymer material with a large specific surface area and adsorption capacity. It can efficiently adsorb and separate the active ingredients in traditional Chinese medicine extracts, and achieve the enrichment and purification of the active ingredients through selective adsorption and elution.

[0034] Furthermore, the drying is oven drying or vacuum drying. The drying method of the residue II is oven drying, and the drying method of the eluent after reduced pressure concentration is vacuum drying.

[0035] The above-mentioned D101 macroporous adsorption resin, AB-8 macroporous adsorption resin, and DM130 macroporous adsorption resin belong to the existing macroporous adsorption resins in the art. Although they can separate and purify Chinese medicine extracts, they have poor selectivity for effective components of Chinese medicine, so the purification effect is not good. In order to further optimize the purification effect of macroporous adsorption resin on Chinese medicine extracts, the present invention also provides a macroporous adsorption resin and a preparation method thereof.

[0036] Furthermore, the macroporous adsorption resin is NBS macroporous adsorption resin, which is prepared by suspension polymerization with N-allyl-N-methylbenzenesulfonamide as a monomer, divinylbenzene as a cross-linking agent, azobisisobutyronitrile or benzoyl peroxide as an initiator, toluene or xylene as a porogen, gelatin and polyvinyl alcohol as dispersants.

[0037] Furthermore, the preparation method of the NBS macroporous adsorption resin comprises the following steps:

[0038] (1) Preparation of the aqueous phase: adding a dispersant to deionized water, wherein the weight percentage of the dispersant in the aqueous phase is 0.5 to 2%, to obtain an aqueous phase;

[0039] (2) Preparation of oil phase: The monomer, crosslinking agent, initiator and porogen are uniformly mixed, wherein the amount of the crosslinking agent is 40-60% of the total mass of the monomer and the crosslinking agent, the amount of the initiator is 0.5-1.5% of the total mass of the monomer and the crosslinking agent, and the amount of the porogen is 100-200% of the total mass of the monomer and the crosslinking agent to obtain an oil phase;

[0040] (3) Preparation of macroporous adsorption resin: The oil phase is added to the water phase with a mass ratio of the water phase to the oil phase of (2-4):1, and the temperature is raised to 60-70°C for reaction for 0.5-3 h while stirring, and the temperature is further raised to 80-90°C for reaction for 1-5 h, solid-liquid separation, alcohol washing, water washing, and drying to obtain NBS macroporous adsorption resin.

[0041] Compared with styrene, the macroporous adsorption resin synthesized using N-allyl-N-methylbenzenesulfonamide as a monomer has better selectivity, has an excellent purification effect on the extract prepared by the present invention, can significantly increase the content of effective ingredients in the traditional Chinese medicine package, and thus improve the use effect of the traditional Chinese medicine package.

[0042] Example 1

[0043] The preparation method of the Chinese medicine foot soaking bag comprises the following steps:

[0044] (1) 30 parts of Artemisia argyi, 20 parts of Chuanwu, 20 parts of Notopterygium wilfordii, 15 parts of Tribulus terrestris, 10 parts of Clematis chinensis, 5 parts of Acanthopanax acanthopanax, 5 parts of Lepidium sibiricum, 4 parts of Cynanchum chinense and 4 parts of Asarum were ground and then volatile oil was extracted by supercritical fluid extraction with CO2 as the extractant at an extraction pressure of 20 MPa, an extraction temperature of 50°C and an extraction time of 3 h to obtain volatile oil and residue I.

[0045] (2) The residue I was extracted by refluxing with 70% by volume ethanol solution, with a solid-liquid ratio of 1 g:80 mL, each extraction time was 2 h, the number of extractions was 3, and the extraction was filtered to obtain an extract and a residue II.

[0046] (3) The residue II was dried in an oven at 80°C to constant weight and crushed to obtain powder I.

[0047] (4) The extract was concentrated under reduced pressure at 55° C. until there was no ethanol, and then separated by column chromatography using D101 macroporous adsorption resin. The height of the resin column was 120 cm and the inner diameter was 12 cm. Gradient elution was performed using ethanol solutions with volume concentrations of 20%, 40%, and 60%, and the eluates from the resin columns eluted with 40% and 60% ethanol solutions were collected.

[0048] (5) The eluate was concentrated to dryness under reduced pressure at 55°C, dried under vacuum at 55°C to constant weight, and crushed to obtain powder II.

[0049] (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabrics with a specification of 20 g / bag to obtain a Chinese medicine foot bath bag.

[0050] Example 2

[0051] The preparation method of the Chinese medicine foot soaking bag comprises the following steps:

[0052] (1) After pulverizing 25 parts of Artemisia argyi, 20 parts of Chuanwu, 15 parts of Notopterygium wilfordii, 10 parts of Tribulus terrestris, 10 parts of Clematis chinensis, 5 parts of Acanthopanax acanthopanax, 5 parts of Lepidium sibiricum, 3 parts of Cynanchum chinense and 3 parts of Asarum, the volatile oil was extracted by supercritical fluid extraction with CO2 as the extractant, the extraction pressure was 25 MPa, the extraction temperature was 50°C, the extraction time was 4 h, and the volatile oil and residue I were obtained.

[0053] (2) The residue I was extracted by refluxing with 60% by volume ethanol solution, with a solid-liquid ratio of 1 g:100 mL, each extraction time was 2 h, the extraction times were 2 times, and the extraction was filtered to obtain an extract and a residue II.

[0054] (3) The residue II was dried in an oven at 80°C to constant weight and crushed to obtain powder I.

[0055] (4) The extract was concentrated under reduced pressure at 50° C. until there was no ethanol, and then separated by column chromatography using AB-8 macroporous adsorption resin. The height of the resin column was 120 cm and the inner diameter was 12 cm. Gradient elution was performed using ethanol solutions with volume concentrations of 20%, 40%, and 60%, and the eluates from the resin columns eluted with 40% and 60% ethanol solutions were collected.

[0056] (5) The eluate was concentrated to dryness under reduced pressure at 60°C, dried under vacuum at 55°C to constant weight, and crushed to obtain powder II.

[0057] (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabrics with a specification of 20 g / bag to obtain a Chinese medicine foot bath bag.

[0058] Example 3

[0059] The preparation method of the Chinese medicine foot soaking bag comprises the following steps:

[0060] (1) 30 parts of Artemisia argyi, 20 parts of Chuanwu, 10 parts of Notopterygium wilfordii, 12 parts of Tribulus terrestris, 10 parts of Clematis chinensis, 5 parts of Acanthopanax acanthopanax, 8 parts of Lepidium scutellariae, 4 parts of Cynanchum chinense and 4 parts of Asarum were ground and then subjected to supercritical fluid extraction for volatile oil extraction, with CO2 as the extractant, the extraction pressure was 15 MPa, the extraction temperature was 40°C, the extraction time was 5 h, and volatile oil and residue I were obtained.

[0061] (2) The residue I was extracted with anhydrous ethanol by refluxing, with a solid-liquid ratio of 1 g:50 mL, each extraction time was 1 h, the number of extractions was 3, and the extraction was filtered to obtain an extract and a residue II.

[0062] (3) The residue II was dried in an oven at 80°C to constant weight and crushed to obtain powder I.

[0063] (4) The extract was concentrated under reduced pressure at 55° C. until there was no ethanol, and then separated by column chromatography using DM130 macroporous adsorption resin. The height of the resin column was 120 cm and the inner diameter was 12 cm. Gradient elution was performed using ethanol solutions with volume concentrations of 20%, 40%, and 60%, and the eluates from the resin columns eluted with 40% and 60% ethanol solutions were collected.

[0064] (5) The eluate was concentrated to dryness under reduced pressure at 60°C, dried under vacuum at 55°C to constant weight, and crushed to obtain powder II.

[0065] (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabrics with a specification of 20 g / bag to obtain a Chinese medicine foot bath bag.

[0066] Example 4

[0067] The preparation method of the Chinese medicine foot soaking bag comprises the following steps:

[0068] (1) After pulverizing 40 parts of Artemisia argyi, 15 parts of Chuanwu, 20 parts of Notopterygium wilfordii, 10 parts of Tribulus terrestris, 10 parts of Clematis chinensis, 6 parts of Acanthopanax acanthopanax, 4 parts of Lepidium scutellariae, 4 parts of Cynanchum chinense and 3 parts of Asarum, the volatile oil was extracted by supercritical fluid extraction with CO2 as the extractant, the extraction pressure was 10 MPa, the extraction temperature was 45°C, the extraction time was 5 h, and the volatile oil and residue I were obtained.

[0069] (2) The residue I was extracted by refluxing with a 60% by volume ethanol solution, with a solid-liquid ratio of 1 g:60 mL, each extraction time was 1.5 h, the number of extractions was 2, and the extraction was filtered to obtain an extract and a residue II.

[0070] (3) The residue II was dried in an oven at 80°C to constant weight and crushed to obtain powder I.

[0071] (4) The extract was concentrated under reduced pressure at 50° C. until there was no ethanol, and then separated by column chromatography using D101 macroporous adsorption resin. The height of the resin column was 120 cm and the inner diameter was 12 cm. Gradient elution was performed using ethanol solutions with volume concentrations of 20%, 40%, and 60%, and the eluates from the resin columns eluted with 40% and 60% ethanol solutions were collected.

[0072] (5) The eluate was concentrated to dryness under reduced pressure at 55°C, dried under vacuum at 55°C to constant weight, and crushed to obtain powder II.

[0073] (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabrics with a specification of 20 g / bag to obtain a Chinese medicine foot bath bag.

[0074] Example 5

[0075] The preparation method of the Chinese medicine foot soaking bag comprises the following steps:

[0076] (1) After pulverizing 20 parts of Artemisia argyi, 20 parts of Chuanwu, 20 parts of Notopterygium wilfordii, 10 parts of Tribulus terrestris, 8 parts of Clematis chinensis, 6 parts of Acanthopanax acanthopanax, 4 parts of Lepidium scutellariae, 4 parts of Cynanchum chinense and 5 parts of Asarum, the volatile oil was extracted by supercritical fluid extraction with CO2 as the extractant, the extraction pressure was 30 MPa, the extraction temperature was 40°C, the extraction time was 3 h, and the volatile oil and residue I were obtained.

[0077] (2) The residue I was extracted by refluxing with 70% by volume ethanol solution, with a solid-liquid ratio of 1 g:100 mL, each extraction time was 1 h, the number of extractions was 3, and the extraction was filtered to obtain an extract and a residue II.

[0078] (3) The residue II was dried in an oven at 80°C to constant weight and crushed to obtain powder I.

[0079] (4) The extract was concentrated under reduced pressure at 55° C. until there was no ethanol, and then separated by column chromatography using D101 macroporous adsorption resin. The height of the resin column was 120 cm and the inner diameter was 12 cm. Gradient elution was performed using ethanol solutions with volume concentrations of 20%, 40%, and 60%, and the eluates from the resin columns eluted with 40% and 60% ethanol solutions were collected.

[0080] (5) The eluate was concentrated to dryness under reduced pressure at 60°C, dried under vacuum at 55°C to constant weight, and crushed to obtain powder II.

[0081] (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabrics with a specification of 20 g / bag to obtain a Chinese medicine foot bath bag.

[0082] Comparative Example 1

[0083] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that the herb is not added and the amount of the herb is superimposed on that of the wormwood leaf.

[0084] Comparative Example 2

[0085] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that the white tribulus terrestris is not added, and the amount of white tribulus terrestris is superimposed on the wormwood leaf.

[0086] Comparative Example 3

[0087] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that Tribulus terrestris and Leprosy Herba are not added, and the amount of Tribulus terrestris and Leprosy Herba is superimposed on that of Artemisia argyi.

[0088] Example 6

[0089] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that the D101 macroporous adsorption resin in Example 1 is replaced by NBS macroporous adsorption resin.

[0090] The preparation method of NBS macroporous adsorption resin comprises the following steps:

[0091] (1) Preparation of the aqueous phase: gelatin and polyvinyl alcohol are added to deionized water, with the weight percentage of gelatin in the aqueous phase being 1% and the weight percentage of polyvinyl alcohol in the aqueous phase being 0.5%, to obtain an aqueous phase.

[0092] (2) Preparation of oil phase: 40 g of N-allyl-N-methylbenzenesulfonamide, 60 g of divinylbenzene, 1 g of azobisisobutyronitrile and 150 g of toluene were mixed uniformly to obtain an oil phase.

[0093] (3) Preparation of macroporous adsorption resin: The oil phase was added to the water phase with a mass ratio of 2:1 between the water phase and the oil phase. The mixture was heated to 60°C for reaction for 0.5 h under stirring. The mixture was further heated to 90°C for reaction for 5 h. The mixture was separated into solid and liquid, washed with ethanol and deionized water, and dried under vacuum at 55°C to constant weight to obtain NBS macroporous adsorption resin.

[0094] Example 7

[0095] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that the D101 macroporous adsorption resin in Example 1 is replaced by NBS macroporous adsorption resin.

[0096] The preparation method of NBS macroporous adsorption resin comprises the following steps:

[0097] (1) Preparation of the aqueous phase: gelatin and polyvinyl alcohol are added to deionized water, with the weight percentage of gelatin in the aqueous phase being 1.5% and the weight percentage of polyvinyl alcohol in the aqueous phase being 0.5%, to obtain an aqueous phase.

[0098] (2) Preparation of oil phase: 50 g of N-allyl-N-methylbenzenesulfonamide, 50 g of divinylbenzene, 1.5 g of azobisisobutyronitrile and 200 g of toluene were uniformly mixed to obtain an oil phase.

[0099] (3) Preparation of macroporous adsorption resin: The oil phase was added to the water phase with a mass ratio of 4:1 between the water phase and the oil phase. The mixture was heated to 65°C for reaction for 1 h under stirring. The mixture was further heated to 85°C for reaction for 4 h. The mixture was separated into solid and liquid, washed with ethanol and deionized water, and dried under vacuum at 55°C to constant weight to obtain NBS macroporous adsorption resin.

[0100] Example 8

[0101] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 1, except that the D101 macroporous adsorption resin in Example 1 is replaced by NBS macroporous adsorption resin.

[0102] The preparation method of NBS macroporous adsorption resin comprises the following steps:

[0103] (1) Preparation of the aqueous phase: gelatin and polyvinyl alcohol are added to deionized water, wherein the weight percentage of gelatin in the aqueous phase is 0.5% and the weight percentage of polyvinyl alcohol in the aqueous phase is 1%, to obtain an aqueous phase.

[0104] (2) Preparation of oil phase: 45 g of N-allyl-N-methylbenzenesulfonamide, 55 g of divinylbenzene, 1.5 g of azobisisobutyronitrile and 180 g of toluene were mixed uniformly to obtain an oil phase.

[0105] (3) Preparation of macroporous adsorption resin: The oil phase was added to the water phase with a mass ratio of 3:1 between the water phase and the oil phase. The mixture was heated to 70°C for reaction for 1 h while stirring. The mixture was then heated to 90°C for reaction for 3 h. The mixture was separated into solid and liquid and washed with ethanol and deionized water. The mixture was dried under vacuum at 55°C until constant weight was obtained to obtain NBS macroporous adsorption resin.

[0106] Comparative Example 4

[0107] The preparation method of the Chinese medicine foot bath bag is the same as that of Example 8, except that the monomer used to prepare the NBS macroporous adsorption resin in Example 8 is replaced by styrene instead of N-allyl-N-methylbenzenesulfonamide.

[0108] 600 patients with symptoms such as cold hands and feet, fear of cold, deficiency of qi and blood, weak spleen and stomach, dysmenorrhea, numbness of lower limbs, etc. were selected and randomly divided into 12 groups, 50 in each group, and the foot soaking bags prepared in the above embodiments 1 to 8 and comparative examples 1 to 4 were used respectively. One bag was used each time, and the feet were soaked once a day for 20 minutes each time (40°C hot water). One course of treatment was 7 days, and 8 courses of treatment were continued. The results are shown in Table 1.

[0109] Efficacy judgment:

[0110] Cure: All symptoms disappear;

[0111] Improvement: symptoms are significantly alleviated;

[0112] Ineffective: No significant change in symptoms.

[0113] Table 1

[0114]

[0115]

[0116] As can be seen from Table 1, compared with Comparative Examples 1-3, Examples 1-5 achieve outstanding effects of warming the meridians, dispelling cold, and dispelling wind and dampness by adopting a Chinese medicine formula composed of a specific ratio of Artemisia argyi, Chuanwu, Notopterygium wilfordii, Tribulus terrestris, Clematis chinensis, Acanthopanax acanthopanax, Leprosy herb, Cynanchum chinense and Asarum; compared with Example 1 and Comparative Example 4, Examples 6 to 8 can further improve the effects of warming the meridians, dispelling cold, and dispelling wind and dampness of the prepared foot bath packs by separating and purifying the Chinese medicine extracts using the NBS macroporous adsorption resin described in the present invention.

[0117] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A Chinese medicine foot bath bag with the effects of warming the meridians and dispelling cold and removing wind and dampness, characterized in that: Made from the following Chinese medicines in parts by weight: 20-40 servings of mugwort leaves 10-30 servings of Chuanwu 10-30 servings of Notopterygium wilfordii 5-20 servings of Tribulus terrestris 5-20 servings of Clematis 1-10 servings of Acanthopanax Cortex 1-10 parts of scutellaria baicalensis Xu Changqing 1-10 parts Asarum 1-10 parts; The preparation method of the Chinese medicine foot soaking bag comprises the following steps: (1) crushing wormwood leaf, chuanwu, notopterygium root, tribulus terrestris, clematis root, acanthopanax bark, leucophylla, cynanchum wilfordii and asarum to extract volatile oil by supercritical fluid extraction to obtain extracted oil and residue I; (2) extracting the residue I with an ethanol solution under reflux, separating the solid and the liquid, and obtaining an extract and a residue II; (3) drying and crushing the residue II to obtain powder I; (4) concentrating the extract under reduced pressure, performing column chromatography separation using a macroporous adsorption resin, and collecting the eluate; (5) The eluate is concentrated under reduced pressure, dried, and crushed to obtain powder II; (6) The volatile oil, powder I and powder II are fully mixed and packaged with non-woven fabric to obtain a traditional Chinese medicine foot bathing bag; The column chromatography separation uses an ethanol solution with a volume concentration of 20-60% for gradient elution, and collects the eluate of the resin column eluted with 40% and 60% ethanol solutions.

2. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The supercritical fluid extraction method uses CO2 as an extractant, the extraction pressure is 10-30 MPa, the extraction temperature is 30-50°C, and the extraction time is 0.5-5 h.

3. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The volume fraction of the ethanol solution is 60-100%.

4. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The solid-liquid ratio of the reflux extraction is 1 g: (20-100) mL, the extraction time is 0.5-2 h each time, and the number of extractions is 1-3 times.

5. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The solid-liquid separation is filtration or centrifugation.

6. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The temperature of the reduced pressure concentration is 55-65°C.

7. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The macroporous adsorption resin is one of D101 macroporous adsorption resin, AB-8 macroporous adsorption resin and DM130 macroporous adsorption resin.

8. The Chinese medicine foot soaking bag with the effects of warming the meridians, dispelling cold, and removing wind and dampness according to claim 1, characterized in that: The drying is oven drying or vacuum drying.

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