A method for optimizing protein translation elongation process to improve protein production in saccharomyces cerevisiae

By integrating eukaryotic translation elongation factor genes into the Saccharomyces cerevisiae genome, the protein translation elongation process was optimized, solving the problem of insufficient protein synthesis capacity in Saccharomyces cerevisiae and achieving efficient synthesis and high yield of Saccharomyces cerevisiae proteins.

CN118909815BActive Publication Date: 2026-07-03JIANGNAN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Filing Date
2024-08-23
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing breeding techniques are insufficient to effectively improve the protein synthesis capacity of Saccharomyces cerevisiae and suffer from poor genetic stability.

Method used

By integrating the genes of eukaryotic translation elongation factors Tef1, Eft1, Yef3, and Anb1 into the Saccharomyces cerevisiae genome, the protein translation elongation process was optimized to improve the protein translation rate and global synthesis capacity.

Benefits of technology

It significantly improved the yield and production efficiency of Saccharomyces cerevisiae protein. The protein content of the engineered strain increased from 45.63g/100g to 48.06g/100g, and the protein yield increased from 3.23g/L to 3.46g/L.

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Abstract

The application discloses a method for optimizing protein translation extension process to improve protein yield of saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. The application improves the global protein synthesis capacity of saccharomyces cerevisiae by regulating the translation elongation factor in the translation initiation process, so that the protein content of saccharomyces cerevisiae is increased from 45.63 g / 100 g to 48.06 g / 100 g, and the protein yield is increased from 3.23 g / L to 3.46 g / L. The application provides technical support for low-cost and green single-cell protein production, and has a wide application prospect in the field of protein substitution.
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