A method for preparing a pest repellent for aquaculture

Abstract

The present invention provides a method for preparing a pest repellent for aquaculture, and relates to the field of aquaculture pest repellents. The method for preparing a pest repellent for aquaculture comprises the following steps: step 1: preparation of repellent, taking 70g of zinc sulfate and 30g of trichloroisocyanuric acid, putting them into a mixing tank, mixing them in proportion and slowly stirring them evenly to obtain a preliminary repellent; step 2: preparation of additives, extracting medicinal material powder by acid method or alkali method to obtain additives; step 3: preparation of composite agents. The toxicity caused by excessive use of zinc sulfate is weakened by adding a very small amount of citric acid, and the heavy metal salt repellent of zinc sulfate is reduced by organic acid, so that when using the repellent, personnel can easily control the dosage, and also reduce the harm of zinc sulfate to aquatic cultured organisms. At the same time, the addition and use of additives and growth promoters make up for the problem of insufficient efficacy in eliminating and repelling pests after the toxicity of zinc sulfate is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture pest repellents, and in particular to a method for preparing a pest repellent for aquaculture. Background Art

[0002] Aquaculture is the use of waters available for breeding (including planting) by humans, according to the ecological habits of the breeding objects and the requirements of the water environment, using aquaculture technology and facilities to engage in the breeding of aquatic economic animals and plants. It is one of the agricultural production sectors, and is divided into marine aquaculture and freshwater aquaculture according to the nature of the waters. According to the breeding and planting objects, it is divided into fish, shrimps and crabs, shellfish, algae, water chestnuts, lotus, lotus roots, etc.

[0003] With the deterioration of the water environment for aquaculture, eutrophication of water bodies, massive breeding of phytoplankton and zooplankton, and frequent occurrence of aquatic animal diseases, the greatest impact is the zooplankton in the water and the parasites on the surface of fish. Among the parasites, crustaceans are the most influential and difficult to kill, and their recurrence is large. The insect bodies and dirt hinder the circulation of water between the gill filaments and the gas exchange on the gill surface, reducing the tolerance of shrimp to hypoxia, and they are easily suffocated to death. Sick adult shrimp or larvae swim slowly, have reduced feeding ability, stop growing and developing, and cannot molt, which further promotes the attachment and proliferation of sessile ciliates, resulting in the death of a large number of hosts.

[0004] To solve the above problems, people generally use spraying zinc sulfate products to inhibit the growth and reproduction of zooplankton and parasites on the surface of fish. However, due to the frequent and large-scale use of zinc sulfate in the prevention and control of sessile ciliates, its usage concentration has gradually increased from 0.3 mg / L to 2.8 mg / L. The high concentration of zinc sulfate, even for a short period of time, will cause almost all animals to have varying degrees of stress detoxification reactions. In addition, long-term use of lipoic acid has a greater toxic effect on the larvae of aquatic animals, and will kill some planktonic bait organisms, destroying the balance of the water environment. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a method for preparing a pest repellent for aquaculture, which solves the problem in the prior art that excessive use of existing repellents is highly toxic and easily causes an imbalance in the water environment.

[0006] To achieve the above object, the present invention is implemented by the following technical scheme: A method for preparing a pest repellent for aquaculture, comprising the following steps:

[0007] Step 1: Preparation of repellent: 70 g of zinc sulfate and 30 g of trichloroisocyanuric acid are put into a mixing tank, mixed according to the proportion and slowly stirred to obtain a preliminary repellent;

[0008] Step 2: Preparation of additives: extracting medicinal powder by acid or alkali method to obtain additives;

[0009] Step 3: Preparation of a composite agent, injecting the repellent in step 1 and the additive in step 2 into a mixing tank, mixing for 5-10 minutes, then adding a growth promoter, and mixing to obtain a composite agent. The composite agent formed by mixing the citric acid contained in the growth promoter is sprayed in water to act on the body, and can also alleviate mycotoxin poisoning, and inhibit aquatic pathogens such as Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens, further improving the overall functionality and wide application of the repellent;

[0010] Step 4: Preparation of finished agent: Mix the compound agent with water in a ratio of 1:60-1:100 to obtain a finished repellent;

[0011] The toxicity caused by excessive use of zinc sulfate is weakened by adding a very small amount of citric acid, and the heavy metal salt repellent of zinc sulfate is reduced by organic acid, so that when using the repellent, personnel can easily control the dosage, and also reduce the harm of zinc sulfate to aquatic cultured organisms. At the same time, the addition of additives and growth promoters can make up for the problem of insufficient effectiveness in eliminating and repelling pests after the toxicity of zinc sulfate is reduced.

[0012] Preferably, the trichloroisocyanuric acid in step 1 contains 7.5% available chlorine.

[0013] Preferably, the growth promoter in step three includes sulfamethoxazole, furazolidone, oxytetracycline and natural growth promoters.

[0014] Preferably, the natural growth promoter includes yucca extract, citric acid and betaine. Yucca as a feed additive also shows a growth-promoting effect.

[0015] Preferably, the weight ratio of the components of the growth promoter is 1%-1.5% of sulfamethoxazole, 0.1%-0.5% of furazolidone, and 1%-3% of oxytetracycline based on the total weight.

[0016] Preferably, the weight ratio of the components of the natural growth promoter is 0.1%-0.3% of yucca extract, 1%-5% of citric acid, and 1%-10% of betaine based on the total weight.

[0017] Preferably, the Chinese medicinal material powder in step 2 includes one or more of Tripterygium wilfordii and Phellodendron chinense.

[0018] Preferably, the two extraction methods in step 2 are as follows:

[0019] (1) When the medicinal material powder is Phellodendron chinense, it is extracted by alkaline method. The raw drug is moistened with 8% lime milk, loaded into a percolation tank, and soaked in saturated lime water for three hours. The speed is adjusted until the percolation is completed in 24 hours. A total of six times the amount of the raw drug is collected. Salt is added to the percolation liquid, and the saturated percolation liquid is left overnight to precipitate berberine. The obtained precipitate is dissolved in boiling water, and the filtrate is filtered while hot. The pH value is adjusted to 1 to 2 with concentrated hydrochloric acid. After the precipitate is obtained, it is recrystallized with 70% alcohol to obtain fine berberine.

[0020] (2) When the medicinal material powder is made of three needles, it is extracted by acid method. It is moistened with 1% sulfuric acid solution. After standing for one hour, it is placed in a percolation tank and soaked in 1% sulfuric acid solution for 24 hours before percolation. A total of eight times the amount of percolation liquid of the raw medicine is collected. The last five times the amount is reserved for the next batch. The first three times the amount of percolation liquid is used. The pH is adjusted to 7 with lime milk to precipitate impurities. The filtrate is filtered with a double-layer cloth bag and then adjusted to a pH of 1 to 2 with concentrated hydrochloric acid. Sodium chloride is added to make the salt content of the solution reach 10%. It is left for 1 to 3 days. After berberine hydrochloride is precipitated, the supernatant is poured off and the lower layer is drained to obtain crude berberine hydrochloride. The crude berberine hydrochloride can be crystallized with 70% ethanol to improve the purity.

[0021] Berberine can regulate the immune system of aquatic animals and help enhance disease resistance. It is effective against many common Gram-negative and Gram-positive bacteria in aquaculture, including Vibrio, Aeromonas, Streptococcus and Pseudomonas. It can inhibit bacterial growth by destroying cell membranes and inhibiting protein synthesis. Since the additive is extracted from medicinal powder and the final extract is berberine, it is a completely natural and extremely safe natural antibiotic, as well as various growth promoters used in combination. Each growth promoter also uses natural growth promoters such as yucca extract, citric acid, and betaine. While being used in combination to repel pests, it will not cause harm to farmed organisms.

[0022] Preferably, the complex formed by mixing the citric acid contained in the growth promoter in step three acts in vitro by spraying water, relieves mycotoxin poisoning, and has an inhibitory effect on aquatic pathogens Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens.

[0023] Preferably, when the finished repellent in step 4 is used, 0.3 g is used for every 1 m³ of water and 200 g is used for every acre of water with a depth of 1 m. After being dissolved in water, it is evenly sprayed throughout the pond at one time. Beneficial Effects

[0024] The present invention provides a method for preparing a pest repellent for aquaculture, which has the following beneficial effects:

[0025] 1. The toxicity caused by excessive use of zinc sulfate is weakened by adding a very small amount of citric acid, and the heavy metal salt repellent of zinc sulfate is degraded by organic acid, so that when using the repellent, personnel can easily control the dosage, and also reduce the harm of zinc sulfate to aquatic organisms. At the same time, the addition of additives and growth promoters makes up for the problem of insufficient effectiveness in eliminating pests after the toxicity of zinc sulfate is reduced.

[0026] 2. Since the additives are extracted from medicinal powder and the final extract is berberine, which is a completely natural and extremely safe natural antibiotic, as well as various growth promoters used in combination, and various growth promoters also use natural growth promoters such as yucca extract, citric acid, and betaine. While being used in combination to repel pests, it will not cause harm to farmed organisms.

[0027] 3. The complex formed by mixing the citric acid contained in the growth promoter acts in vitro by spraying water. It also has the effect of alleviating mycotoxin poisoning and inhibiting aquatic pathogens such as Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens, further improving the overall functionality and wide application of the repellent. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The present invention provides an overall flow chart of a method for preparing a pest repellent for aquaculture. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1:

[0030] like Figure 1 As shown, an embodiment of the present invention provides a method for preparing a pest repellent for aquaculture, comprising the following steps:

[0031] Step 1: Preparation of repellent: 70 g of zinc sulfate and 30 g of trichloroisocyanuric acid are put into a mixing tank, mixed according to the proportion and slowly stirred to obtain a preliminary repellent;

[0032] Step 2: Preparation of additives: extracting medicinal powder by acid or alkali method to obtain additives;

[0033] Step 3: Preparation of the composite agent, injecting the repellent in step 1 and the additive in step 2 into a mixing tank, mixing for 5-10 minutes, then adding the growth promoter, and mixing to obtain the composite agent;

[0034] Step 4: Preparation of finished agent: Mix the compound agent with water in a ratio of 1:60-1:100 to obtain a finished repellent;

[0035] The heavy metal salt repellent of zinc sulfate is degraded by organic acid, so that personnel can easily control the dosage when using the repellent, and also reduce the harm of zinc sulfate to aquatic cultured organisms. At the same time, the addition of additives and growth promoters makes up for the problem of insufficient effectiveness in eliminating and repelling pests after the toxicity of zinc sulfate is reduced.

[0036] In the step 1, the trichloroisocyanuric acid contains 7.5% of available chlorine.

[0037] The growth promoting agent in step three includes natural growth promoting agent.

[0038] The natural growth promoter includes yucca extract, citric acid and betaine. Yucca as a feed additive also shows a growth-promoting effect, which enhances feed digestion and nutrient absorption, improves immunity and high resistance to infectious diseases.

[0039] The weight ratio of each component of the natural growth promoter is 0.1%-0.3% of yucca extract, 1%-5% of citric acid, and 1%-10% of betaine based on the total weight.

[0040] The Chinese medicinal material powder in step 2 includes one or more of trifoliate needles and phellodendron chinense.

[0041] The two extraction methods in step 2 are as follows:

[0042] (1) When the medicinal material powder is Phellodendron chinense, it is extracted by alkaline method. The raw drug is moistened with 8% lime milk, loaded into a percolation tank, and soaked in saturated lime water for three hours. The speed is adjusted until the percolation is completed in 24 hours. A total of six times the amount of the raw drug is collected. Salt is added to the percolation liquid, and the saturated percolation liquid is left overnight to precipitate berberine. The obtained precipitate is dissolved in boiling water, and the filtrate is filtered while hot. The pH value is adjusted to 1 to 2 with concentrated hydrochloric acid. After the precipitate is obtained, it is recrystallized with 70% alcohol to obtain fine berberine.

[0043] (2) When the medicinal material powder is made of three needles, it is extracted by acid method. It is moistened with 1% sulfuric acid solution. After standing for one hour, it is placed in a percolation tank and soaked in 1% sulfuric acid solution for 24 hours before percolation. A total of eight times the amount of percolation liquid of the raw medicine is collected. The last five times the amount is reserved for the next batch. The first three times the amount of percolation liquid is used. The pH is adjusted to 7 with lime milk to precipitate impurities. The filtrate is filtered with a double-layer cloth bag and then adjusted to a pH of 1 to 2 with concentrated hydrochloric acid. Sodium chloride is added to make the salt content of the solution reach 10%. It is left for 1 to 3 days. After berberine hydrochloride is precipitated, the supernatant is poured off and the lower layer is drained to obtain crude berberine hydrochloride. The crude berberine hydrochloride can be crystallized with 70% ethanol to improve the purity.

[0044] Berberine can regulate the immune system of aquatic animals, help enhance disease resistance, stimulate the activity of immune cells and increase the production of antioxidants. It is effective against many Gram-negative and Gram-positive bacteria commonly found in aquaculture, including Vibrio, Aeromonas, Streptococcus and Pseudomonas. It can inhibit bacterial growth by destroying cell membranes and inhibiting protein synthesis, and can improve water quality by inhibiting the growth of algae and harmful microorganisms.

[0045] The composite agent formed by mixing the citric acid contained in the growth promoter in step three acts in vitro by spraying water, relieves mycotoxin poisoning, and has an inhibitory effect on aquatic pathogens such as Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens.

[0046] When the finished repellent in step 4 is used, 0.3 g is used for every 1 m³ of water and 200 g is used for every acre of water with a depth of 1 m. After being dissolved in water, it is evenly sprayed throughout the pond at one time. Embodiment 2:

[0047] Based on the method for preparing a pest repellent for aquaculture provided in the first embodiment of the present application, the second embodiment of the present application proposes another method for preparing a pest repellent for aquaculture. The second embodiment is only a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0048] Specifically, the method for preparing a pest repellent for aquaculture provided in the second embodiment of the present application is different in that it includes the following steps:

[0049] Step 1: Preparation of repellent: 70 g of zinc sulfate and 30 g of trichloroisocyanuric acid are put into a mixing tank, mixed according to the proportion and slowly stirred to obtain a preliminary repellent;

[0050] Step 2: Preparation of additives: extracting medicinal powder by acid or alkali method to obtain additives;

[0051] Step 3: Preparation of the composite agent, injecting the repellent in step 1 and the additive in step 2 into a mixing tank, mixing for 5-10 minutes, then adding the growth promoter, and mixing to obtain the composite agent;

[0052] Step 4: Preparation of finished agent: mix the compound agent with water in a ratio of 1:60-1:100 to obtain a finished repellent.

[0053] In the step 1, the trichloroisocyanuric acid contains 7.5% of available chlorine.

[0054] The growth promoter in step three includes one or more combinations of sulfamethoxazole, furazolidone, and oxytetracycline.

[0055] The weight ratio of each component of the growth promoter is 1%-1.5% of sulfamethoxazole, 0.1%-0.5% of furazolidone, and 1%-3% of oxytetracycline based on the total weight.

[0056] The Chinese medicinal material powder in step 2 includes one or more of trifoliate needles and phellodendron chinense.

[0057] The two extraction methods in step 2 are as follows:

[0058] (1) When the medicinal material powder is Phellodendron chinense, it is extracted by alkaline method. The raw drug is moistened with 8% lime milk, loaded into a percolation tank, and soaked in saturated lime water for three hours. The speed is adjusted until the percolation is completed in 24 hours. A total of six times the amount of the raw drug is collected. Salt is added to the percolation liquid, and the saturated percolation liquid is left overnight to precipitate berberine. The obtained precipitate is dissolved in boiling water, and the filtrate is filtered while hot. The pH value is adjusted to 1 to 2 with concentrated hydrochloric acid. After the precipitate is obtained, it is recrystallized with 70% alcohol to obtain fine berberine.

[0059] (2) When the medicinal material powder is made of three needles, it is extracted by acid method. It is moistened with 1% sulfuric acid solution. After standing for one hour, it is placed in a percolation tank and soaked in 1% sulfuric acid solution for 24 hours before percolation. A total of eight times the amount of percolation liquid of the raw drug is collected. The last five times the amount is kept for the next batch. The first three times the amount of percolation liquid is used. The pH is adjusted to 7 with lime milk to precipitate impurities. The filtrate is filtered with a double-layer cloth bag and then adjusted to a pH of 1 to 2 with concentrated hydrochloric acid. Sodium chloride is added to make the salt content of the solution reach 10%. It is left for 1 to 3 days. After berberine hydrochloride is precipitated, the supernatant is poured off and the lower layer is drained to obtain crude berberine hydrochloride. The crude berberine hydrochloride can be crystallized with 70% ethanol to improve the purity.

[0060] When the finished repellent in step 4 is used, 0.3 g is used for every 1 m³ of water and 200 g is used for every acre of water with a depth of 1 m. After being dissolved in water, it is evenly sprayed throughout the pond at one time. Embodiment three:

[0061] Based on the method for preparing a pest repellent for aquaculture provided in the first embodiment of the present application, the third embodiment of the present application proposes another method for preparing a pest repellent for aquaculture. The third embodiment is only a preferred embodiment of the first embodiment, and the implementation of the third embodiment will not affect the independent implementation of the first embodiment.

[0062] Specifically, the method for preparing a pest repellent for aquaculture provided in the third embodiment of the present application is different in that it includes the following steps:

[0063] Step 1: Preparation of repellent: 70 g of zinc sulfate and 30 g of trichloroisocyanuric acid are put into a mixing tank, mixed according to the proportion and slowly stirred to obtain a preliminary repellent;

[0064] Step 2: Preparation of additives: extracting medicinal powder by acid or alkali method to obtain additives;

[0065] Step 3: Preparation of the finished agent: Mix the additive with the preliminary repellent, and then mix the mixture with water in a ratio of 1:60-1:100 to obtain the finished repellent.

[0066] In the step 1, the trichloroisocyanuric acid contains 7.5% of available chlorine.

[0067] The Chinese medicinal material powder in step 2 includes one or more of trifoliate needles and phellodendron chinense.

[0068] The two extraction methods in step 2 are as follows:

[0069] (1) When the medicinal material powder is Phellodendron chinense, it is extracted by alkaline method. The raw drug is moistened with 8% lime milk, loaded into a percolation tank, and soaked in saturated lime water for three hours. The speed is adjusted until the percolation is completed in 24 hours. A total of six times the amount of the raw drug is collected. Salt is added to the percolation liquid, and the saturated percolation liquid is left overnight to precipitate berberine. The obtained precipitate is dissolved in boiling water, and the filtrate is filtered while hot. The pH value is adjusted to 1 to 2 with concentrated hydrochloric acid. After the precipitate is obtained, it is recrystallized with 70% alcohol to obtain fine berberine.

[0070] (2) When the medicinal material powder is made of three needles, it is extracted by acid method. It is moistened with 1% sulfuric acid solution. After standing for one hour, it is placed in a percolation tank and soaked in 1% sulfuric acid solution for 24 hours before percolation. A total of eight times the amount of percolation liquid of the raw drug is collected. The last five times the amount is kept for the next batch. The first three times the amount of percolation liquid is used. The pH is adjusted to 7 with lime milk to precipitate impurities. The filtrate is filtered with a double-layer cloth bag and then adjusted to a pH of 1 to 2 with concentrated hydrochloric acid. Sodium chloride is added to make the salt content of the solution reach 10%. It is left for 1 to 3 days. After berberine hydrochloride is precipitated, the supernatant is poured off and the lower layer is drained to obtain crude berberine hydrochloride. The crude berberine hydrochloride can be crystallized with 70% ethanol to improve the purity.

[0071] When the finished repellent in step 3 is used, 0.3 g is used for every 1 m³ of water and 200 g is used for every acre of water with a depth of 1 m. After being dissolved in water, it is evenly sprayed throughout the pond at one time.

[0072] Table 1 Tests of antibacterial types, duration and toxicity:

[0073] Antibacterial species Duration (days) Toxicity Embodiment 1 9 7 Low Embodiment 2 7 2-5 middle Embodiment 3 3 1-3 high

[0074] From the test results in the above table, it can be seen that the types and duration of antibacterial effects of pest repellents used in aquaculture are significantly better, while also being less toxic.

[0075] The present invention provides a method for preparing a pest repellent for aquaculture. When the method is operated, firstly, 70g of zinc sulfate and 30g of trichloroisocyanuric acid are taken, put into a mixing tank, mixed according to a proportion and slowly stirred to obtain a preliminary repellent. The zinc ions generated by zinc sulfate in water are combined with proteins of insect cells to form protein salts, which are precipitated. At the same time, the zinc ions are easily combined with the bases of insect cell enzymes. The sulfhydryl group is the active group of the enzyme, and it loses its function after combining with the zinc ions, thereby causing the death of parasites, thereby achieving the effect of repelling and killing pests. At the same time, the trichloroisocyanuric acid can destroy proteins and enzymes in microbial cells, thereby killing bacteria, viruses and plankton in water, effectively reducing the organic matter in the water. The content of pollutants such as pollutants and nitrogen and phosphorus is maintained to maintain the ecological balance of the water body and keep the water clear and transparent. Then, the additive is extracted by acid or alkali method. Berberine is obtained by extracting Phellodendron chinense or Tripterygium wilfordii. Berberine can regulate the immune system of aquatic animals and help enhance disease resistance. It can stimulate the activity of immune cells and increase the production of antioxidants. It is effective against many Gram-negative and Gram-positive bacteria commonly found in aquaculture, including Vibrio, Aeromonas, Streptococcus and Pseudomonas. It can inhibit bacterial growth by destroying cell membranes and inhibiting protein synthesis. Berberine can also effectively fight against a variety of aquatic fungal pathogens, including Saprolegnia, and can inhibit fungal growth by inhibiting the synthesis of ergosterol, an important component of fungal cell membranes. At the same time, it can also improve water quality by inhibiting the growth of algae and harmful microorganisms. Since the additive is extracted from medicinal powder and the final extract is berberine, it is a completely natural and extremely safe natural antibiotic that can regulate the immune system of aquatic animals and help enhance disease resistance. It can stimulate the activity of immune cells and increase the production of antioxidants, as well as various growth promoters used in combination. Each growth promoter also uses natural growth promoters such as yucca extract, citric acid, and betaine. While achieving the coordinated use of repellent pests, it will not cause harm to farmed organisms. Then, inject the repellent in step one and the additive in step two into the mixing tank, mix for 5-10 minutes, and then add the growth promoter. After combination, a composite agent is obtained. The yucca added to the growth promoter as a feed additive also shows a growth-promoting effect. It enhances feed digestion and nutrient absorption, improves immunity and high resistance to infectious diseases. The citric acid therein has the effects of alleviating mycotoxin poisoning, and inhibiting aquatic pathogens Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens, further improving the overall functionality and wide application of the repellent. The heavy metal salt repellent of zinc sulfate is degraded by organic acid, so that when using the repellent, personnel can easily control the dosage, and also reduce the harm of zinc sulfate to aquatic cultured organisms. Finally, the composite agent is mixed with water in a ratio of 1:60-1:100 to obtain a finished repellent.

[0076] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a pest repellent for aquaculture, characterized in that: The following steps are involved: Step 1: Preparation of repellent: 70 g of zinc sulfate and 30 g of trichloroisocyanuric acid are put into a mixing tank, mixed according to the proportion and slowly stirred to obtain a preliminary repellent; Step 2: Preparation of additives: extracting medicinal powder by acid or alkali method to obtain additives; The Chinese medicinal material powder in step 2 includes one or more of trifoliate needles and phellodendron chinense; The two extraction methods in step 2 are as follows: (1) When the medicinal material powder is Phellodendron chinense, an alkali method is used for extraction. The raw drug is moistened with 8% lime milk, placed in a percolation tank, and soaked in saturated lime water for three hours. The speed is adjusted until the percolation is completed in 24 hours. A total of six times the amount of the raw drug is collected. Salt is added to the percolation liquid, and the saturated liquid is left overnight to precipitate berberine. The precipitate is dissolved in boiling water, and the filtrate is filtered while hot. The pH value is adjusted to 1 to 2 with concentrated hydrochloric acid. After the precipitate is obtained, it is recrystallized with 70% alcohol to obtain fine berberine. (2) When the medicinal material powder is made of three needles, it is extracted by acid method. It is moistened with 1% sulfuric acid solution. After standing for one hour, it is placed in a percolation tank and soaked in 1% sulfuric acid solution for 24 hours before percolation. A total of eight times the amount of percolation liquid of the raw medicine is collected. The last five times the amount is reserved for the next batch. The first three times the amount of percolation liquid is used. The pH is adjusted to 7 with lime milk to precipitate impurities. The filtrate is filtered with a double-layer cloth bag and then adjusted to pH 1 to 2 with concentrated hydrochloric acid. Sodium chloride is added to make the salt content of the solution reach 10%. It is left for 1 to 3 days. After berberine hydrochloride is precipitated, the supernatant is poured off and the lower layer is drained to obtain crude berberine hydrochloride. The crude berberine hydrochloride is then crystallized with 70% ethanol to improve the purity. Step 3: Preparation of the composite agent, injecting the repellent in step 1 and the additive in step 2 into a mixing tank, mixing for 5-10 minutes, then adding the growth promoter, and mixing to obtain the composite agent; The growth promoter in step 3 includes sulfamethoxazole, furazolidone, oxytetracycline and natural growth promoter; The natural growth promoter includes yucca extract, citric acid and betaine; The composite agent formed by mixing the citric acid contained in the growth promoter in step 3 acts on the body by spraying water, relieves mycotoxin poisoning, and has an inhibitory effect on aquatic pathogens such as Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas fluorescens; Step 4: Preparation of finished agent: mix the compound agent with water in a ratio of 1:60-1:100 to obtain a finished repellent.

2. The method for preparing a pest repellent for aquaculture according to claim 1, characterized in that: In the step 1, the trichloroisocyanuric acid contains 7.5% of available chlorine.

3. The method for preparing a pest repellent for aquaculture according to claim 1, characterized in that: The weight ratio of each component of the growth promoter is 1%-1.5% of sulfamethoxazole, 0.1%-0.5% of furazolidone, and 1%-3% of oxytetracycline based on the total weight.

4. The method for preparing a pest repellent for aquaculture according to claim 1, characterized in that: The weight ratio of each component of the natural growth promoter is 0.1%-0.3% of yucca extract, 1%-5% of citric acid, and 1%-10% of betaine based on the total weight.

5. The method for preparing a pest repellent for aquaculture according to claim 1, characterized in that: When the finished repellent in step 4 is used, 0.3 g is used for every 1 m³ of water and 200 g is used for every acre of water with a depth of 1 m. After being dissolved in water, it is evenly sprayed throughout the pond at one time.

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