Double-antibiotic brightening freeze-dried essence and preparation method thereof

Abstract

The present invention relates to the technical field of skin care products, and specifically discloses a dual-antibiotic brightening freeze-dried essence and a preparation method thereof. The dual-antibiotic brightening freeze-dried essence comprises a freeze-dried powder and a solvent liquid, wherein the ratio of the mass of the freeze-dried powder to the volume of the solvent liquid is 100-130 mg: 2-3 ml, and the freeze-dried powder is prepared by mixing and freeze-drying the following components: a solvent, a skin conditioner, an antioxidant, and the solvent liquid is composed of the following components: a solvent, an anti-glycation agent, a moisturizer, a whitening composition, and a preservative, and the whitening composition is a compound of kava pepper leaf / root / stem extract, madecassoside, Centella asiatica extract, red algae extract, and sea daffodil extract. The present invention has the advantage of better achieving rapid whitening.

Description

Technical Field

[0001] The invention relates to the field of skin care products, and in particular to a double-antibiotic brightening freeze-dried essence and a preparation method thereof. Background Art

[0002] Skin care products are products for skin care. Skin care products have various functions and are used to improve the skin in a targeted manner. Based on consumers' pursuit of whitening, skin care products with whitening effects are one of the more popular skin care products. Skin care products with whitening effects usually achieve whitening effects by adding ingredients that can inhibit the activity of tyrosinase to reduce the production of melanin in the skin.

[0003] Although many whitening ingredients have the effect of inhibiting tyrosinase activity in in vitro tests, in actual use, insufficient penetration of ingredients has become a key factor limiting the whitening effect. Many active ingredients have difficulty penetrating the surface of the skin and cannot reach the target site, resulting in a slow whitening effect. It takes a long time to see a slight effect, which makes it difficult to meet consumers' demand for rapid whitening. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to better achieve rapid whitening, the present application provides a dual-antibiotic brightening freeze-dried essence and a preparation method thereof.

[0005] In the first aspect, the present application provides a dual-antibody brightening freeze-dried essence, which adopts the following technical solution:

[0006] A dual-antibody brightening freeze-dried essence, comprising freeze-dried powder and a solvent solution, wherein the ratio of the mass of the freeze-dried powder to the volume of the solvent solution is 100-130 mg: 2-3 ml;

[0007] The freeze-dried powder is prepared by mixing the following components by mass and then freeze-drying:

[0008] Solvent 78-91.4499 parts;

[0009] Skin conditioning agent 5.54775-12.955 parts;

[0010] Antioxidant 3.00235-9.045 parts;

[0011] The solvent liquid is composed of the following components in parts by mass:

[0012] Solvent 81.5502-95.8174 parts;

[0013] Anti-glycation agent 0.2511-2.0088 parts;

[0014] Moisturizer 3.4583-13.217 parts;

[0015] 0.072-2.504 parts of whitening composition;

[0016] Preservative 0.4012-0.72 parts;

[0017] The whitening composition is a compound of Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract and Narcissus chinensis extract.

[0018] By adopting the above technical scheme, the whitening composition is a compound of Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract, and Narcissus chinensis extract, and the mutual cooperation of the active ingredients can play a good role in inhibiting tyrosinase, and can well open the skin channels so that the active ingredients can quickly penetrate into the melanocytes, thereby quickly achieving the effect of inhibiting tyrosinase, and the timeliness of tyrosinase inhibition is long, and frequent use is not required. By adding antioxidants and anti-glycation agents, the antioxidant and anti-glycation effects can be effectively achieved, thereby effectively resisting skin aging, thereby restoring the skin to youth, and making the skin's metabolic function better, thereby accelerating the metabolism of melanin, and achieving rapid whitening and anti-aging and anti-wrinkle effects.

[0019] Preferably, the mass ratio of the Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract and Narcissus oleraceus extract is 0.002-0.004: 0.0475-0.475: 0.0025-0.025: 0.01-1: 0.01-1.

[0020] By adopting the above technical scheme and specifically selecting the mass ratio of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, red algae extract, and sea daffodil extract, the activity of tyrosinase can be better inhibited, the synthesis of melanin can be better reduced, and the rapid whitening effect can be better.

[0021] Preferably, in the freeze-dried powder, the antioxidant is a compound of sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid.

[0022] By adopting the above technical scheme and specifically selecting the antioxidants as a combination of sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid, the antioxidant effect is better, anti-aging is better, the skin is better restored to youth, and the skin's metabolic function is better.

[0023] Preferably, the mass ratio of the sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid is 0.00125-0.025:0.0011-1.02:3-8.

[0024] By adopting the above technical solution and specifically selecting the mass ratio of sodium ascorbate, ascorbic acid, and 3-o-ethyl ascorbic acid, the antioxidant effect is further enhanced, the skin is restored to youthfulness faster, and the effect of enhancing skin metabolism is more significant.

[0025] Preferably, in the solvent solution, the anti-glycation agent is a compound of carnosine, arginine PCA, tetrapeptide-1 and nonapeptide-1.

[0026] By adopting the above technical scheme, by specifically selecting the anti-glycation agent as a combination of carnosine, arginine PCA, tetrapeptide-1, and nonapeptide-1, the anti-glycation effect is better, and it cooperates better with antioxidants, making the anti-aging effect more significant, the skin is better restored to youth, and the metabolic effect is better.

[0027] Preferably, the mass ratio of carnosine, arginine PCA, tetrapeptide-1 and nonapeptide-1 is 0.1-1:0.15-1:0.001-0.008:0.0001-0.0008.

[0028] By adopting the above technical scheme and specifically selecting the mass ratio of carnosine, arginine PCA, tetrapeptide-1 and nonapeptide-1, the anti-glycation effect is better, the skin metabolism function is better, the melanin is metabolized more quickly, and rapid whitening is achieved.

[0029] Preferably, in the freeze-dried powder, the skin conditioning agent is a compound of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch glycolate octenylsuccinate, and silica;

[0030] In the solvent liquid, the humectant is a compound of 1,2-pentanediol, butanediol, 1,2-hexanediol, and hydroxyethylpiperazineethane sulfonic acid;

[0031] In the solvent liquid, the preservative is a compound of p-hydroxyacetophenone and phenoxyethanol.

[0032] By adopting the above technical solution, the skin can be healthier, the skin care effect can be better, and the product quality can be higher.

[0033] Preferably, the mass ratio of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch octenylsuccinate, and silica is 5-10: 0.5-2: 0.0135-0.27: 0.03395-0.679: 0.0003-0.006;

[0034] The mass ratio of 1,2-pentanediol, butanediol, 1,2-hexanediol and hydroxyethylpiperazineethane sulfonic acid is 0.02-0.16: 2.9291-7.3782: 0.4092-0.6788: 0.1-5;

[0035] The mass ratio of p-hydroxyacetophenone to phenoxyethanol is 0.4-0.6:0.0012-0.12.

[0036] By adopting the above technical solution, skin damage can be better repaired, with a very significant skin care effect, and the product efficacy is quite significant.

[0037] In a second aspect, the present application provides a method for preparing a dual-antibody brightening freeze-dried essence, using the following technical solution:

[0038] A method for preparing the above-mentioned dual-antibody brightening freeze-dried essence, including a freeze-dried powder preparation method and a solvent liquid preparation method;

[0039] The preparation method of the freeze-dried powder comprises the following steps:

[0040] Step 1), mixing the components uniformly to obtain a mixture;

[0041] Step 2), freeze-drying the mixture at (-45)-(-55)°C to obtain freeze-dried powder;

[0042] The preparation method of the solvent solution comprises the following steps:

[0043] Step 01), heating the solvent for sterilization;

[0044] Step 02), add the remaining components into a solvent, mix well, and obtain a solvent solution.

[0045] By adopting the above technical scheme, the prepared double-anti-brightening freeze-dried essence has a significant rapid whitening effect, can effectively resist aging, achieve better anti-wrinkle effect, and has a good care effect on the skin.

[0046] In summary, this application has the following beneficial effects:

[0047] 1. Since the whitening composition of the present application is a compound of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract, and Sea Narcissus extract, the active ingredients cooperate with each other to inhibit tyrosinase well, and can open the skin channels well, so that the active ingredients can quickly penetrate into the melanocytes, thereby quickly achieving the effect of inhibiting tyrosinase, and the timeliness of tyrosinase inhibition is long, and frequent use is not required. By adding antioxidants and anti-glycation agents, the antioxidant and anti-glycation effects can be effectively achieved, thereby effectively resisting skin aging, thereby restoring the skin to youth, and making the skin's metabolic function better, thereby accelerating melanin metabolism, and achieving rapid whitening and anti-aging and anti-wrinkle effects.

[0048] 2. In the present application, it is preferred to specifically select the mass ratio of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, red algae extract, and sea daffodil extract to better inhibit the activity of tyrosinase, better reduce melanin synthesis, and achieve a better rapid whitening effect. DETAILED DESCRIPTION

[0049] The present application is further described in detail below with reference to the embodiments.

[0050] Example 1

[0051] A double-antibody brightening freeze-dried essence comprises freeze-dried powder and a solvent liquid. The mass of the freeze-dried powder in each double-antibody brightening freeze-dried essence is 100 mg, and the volume of the solvent liquid is 2 ml.

[0052] The lyophilized powder is prepared by mixing and lyophilizing the following components:

[0053] Solvent, Skin Conditioner, Antioxidant.

[0054] The solvent is deionized water.

[0055] The skin conditioning agent is a compound of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch glycolate octenylsuccinate and silica.

[0056] Mannitol was commercially available, CAS number: 69-65-8.

[0057] Trehalose is commercially available, CAS number: 99-20-7.

[0058] The Haematococcus pluvialis extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0059] Sodium starch octenylsuccinate is commercially available, CAS number: 66829-29-6.

[0060] Silica was purchased from Evonik in Germany, model: SIPERNAT 2200PC.

[0061] The antioxidant is a compound of sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid.

[0062] Sodium ascorbate is commercially available, CAS number: 134-03-2.

[0063] Ascorbic acid was commercially available, CAS number: 299-36-5.

[0064] 3-o-Ethyl ascorbic acid was commercially available, CAS No.: 86404-04-8.

[0065] The preparation method of freeze-dried powder comprises the following steps:

[0066] Step 1), 91.4499 kg of deionized water, 5 kg of mannitol, and 0.5 kg of trehalose are mixed and heated to 85 ° C, kept at 85 ° C, rotated at 120 r / min, stirred for 30 min, cooled to 45 ° C, added with 0.0135 kg of Haematococcus pluvialis extract, 0.03395 kg of sodium octenyl succinate starch, 0.00125 kg of sodium ascorbate, 0.0003 kg of silica, rotated at 120 r / min, stirred for 5 min, added with 3 kg of 3-o-ethyl ascorbic acid, 0.0011 kg of ascorbic acid, rotated at 120 r / min, stirred for 5 min, and filtered with a 500 mesh filter cloth to obtain a mixture.

[0067] Step 2), freeze-drying the mixture at -45°C to obtain freeze-dried powder.

[0068] The solvent liquid consists of the following components:

[0069] Solvent, anti-glycation agent, moisturizer, whitening composition, preservative.

[0070] The solvent is deionized water.

[0071] The anti-glycation agent is a compound of carnosine, arginine PCA, tetrapeptide-1, and nonapeptide-1.

[0072] Carnosine was commercially available, CAS number: 305-84-0.

[0073] Arginine PCA was commercially available, CAS number: 56265-06-6.

[0074] Tetrapeptide-1 is commercially available, CAS number: 1632354-04-1.

[0075] Nonapeptide-1 was commercially available, CAS number: 158563-45-2.

[0076] The moisturizing agent is a compound of 1,2-pentanediol, butylene glycol, 1,2-hexanediol and hydroxyethylpiperazineethane sulfonic acid.

[0077] 1,2-Pentanediol is commercially available, CAS number: 5343-92-0.

[0078] Butanediol is commercially available, CAS number: 110-63-4.

[0079] 1,2-Hexanediol is commercially available, CAS number: 6920-22-5.

[0080] Hydroxyethylpiperazineethanesulfonic acid is commercially available, CAS number: 7365-45-9.

[0081] The whitening composition is a compound of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract and Sea Narcissus extract.

[0082] Kava leaf / root / stem extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0083] Madecassoside is commercially available, CAS number: 34540-22-2.

[0084] Centella asiatica extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0085] The red algae extract was purchased from Guangzhou Ruiran Biotechnology Co., Ltd.

[0086] The sea daffodil extract was purchased from Shaanxi Xinkang Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0087] The preservative is a combination of p-hydroxyacetophenone and phenoxyethanol.

[0088] p-Hydroxyacetophenone is commercially available, CAS number: 99-93-4.

[0089] Phenoxyethanol is commercially available, CAS number: 122-99-6.

[0090] The preparation method of the solvent solution comprises the following steps:

[0091] Step 01), add 95.8174 kg of deionized water into the emulsifying pot and heat to 90°C, keep at 90°C, rotate at 120 r / min, stir for 20 min, and cool down.

[0092] Step 02), deionized water is cooled to 60°C, 0.4kg p-hydroxyacetophenone, 0.0012kg phenoxyethanol, 0.02kg 1,2-pentanediol, 2.9291kg butanediol, 0.4092kg 1,2-hexanediol are added, the speed is 120r / min, stirred for 5min, cooled to 45°C, 0.1kg hydroxyethylpiperazine ethane sulfonic acid, 0.1kg carnosine, 0.15kg arginine PCA, 0.001kg tetrapeptide-1, 0.0001kg nonapeptide-1, 0.002kg kava pepper leaf / root / stem extract, 0.0475kg madecassoside, 0.0025kg Centella asiatica extract, 0.01kg red algae extract, 0.01kg sea daffodil extract, the speed is 120r / min, stirred for 20min, filtered with a 500-mesh filter cloth to obtain a solvent solution.

[0093] Example 2

[0094] A double-antibody brightening freeze-dried essence comprises freeze-dried powder and a solvent liquid. The mass of the freeze-dried powder in each double-antibody brightening freeze-dried essence is 115 mg, and the volume of the solvent liquid is 2.5 ml.

[0095] The lyophilized powder is prepared by mixing and lyophilizing the following components:

[0096] Solvent, Skin Conditioner, Antioxidant.

[0097] The solvent is deionized water.

[0098] The skin conditioning agent is a compound of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch glycolate octenylsuccinate and silica.

[0099] Mannitol was commercially available, CAS number: 69-65-8.

[0100] Trehalose is commercially available, CAS number: 99-20-7.

[0101] The Haematococcus pluvialis extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0102] Sodium starch octenylsuccinate is commercially available, CAS number: 66829-29-6.

[0103] Silica was purchased from Evonik in Germany, model: SIPERNAT 2200PC.

[0104] The antioxidant is a compound of sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid.

[0105] Sodium ascorbate is commercially available, CAS number: 134-03-2.

[0106] Ascorbic acid was commercially available, CAS number: 299-36-5.

[0107] 3-o-Ethyl ascorbic acid was commercially available, CAS No.: 86404-04-8.

[0108] The preparation method of freeze-dried powder comprises the following steps:

[0109] Step 1), 84.72495kg of deionized water, 7.5kg of mannitol, and 1.25kg of trehalose are mixed and heated to 85°C, kept at 85°C, rotated at 120r / min, stirred for 30min, cooled to 45°C, 0.14175kg of Haematococcus pluvialis extract, 0.356475kg of sodium octenyl succinate starch, 0.013125kg of sodium ascorbate, and 0.00315kg of silica are added, rotated at 120r / min, stirred for 5min, 5.5kg of 3-o-ethyl ascorbic acid and 0.51055kg of ascorbic acid are added, rotated at 120r / min, stirred for 5min, and the material is filtered with a 500-mesh filter cloth to obtain a mixture.

[0110] Step 2), freeze-drying the mixture at -50°C to obtain freeze-dried powder.

[0111] The solvent liquid consists of the following components:

[0112] Solvent, anti-glycation agent, moisturizer, whitening composition, preservative.

[0113] The solvent is deionized water.

[0114] The anti-glycation agent is a compound of carnosine, arginine PCA, tetrapeptide-1, and nonapeptide-1.

[0115] Carnosine was commercially available, CAS number: 305-84-0.

[0116] Arginine PCA was commercially available, CAS number: 56265-06-6.

[0117] Tetrapeptide-1 is commercially available, CAS number: 1632354-04-1.

[0118] Nonapeptide-1 was commercially available, CAS number: 158563-45-2.

[0119] The moisturizing agent is a compound of 1,2-pentanediol, butylene glycol, 1,2-hexanediol and hydroxyethylpiperazineethane sulfonic acid.

[0120] 1,2-Pentanediol is commercially available, CAS number: 5343-92-0.

[0121] Butanediol is commercially available, CAS number: 110-63-4.

[0122] 1,2-Hexanediol is commercially available, CAS number: 6920-22-5.

[0123] Hydroxyethylpiperazineethanesulfonic acid is commercially available, CAS number: 7365-45-9.

[0124] The whitening composition is a compound of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract and Sea Narcissus extract.

[0125] Kava leaf / root / stem extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0126] Madecassoside is commercially available, CAS number: 34540-22-2.

[0127] Centella asiatica extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0128] The red algae extract was purchased from Guangzhou Ruiran Biotechnology Co., Ltd.

[0129] The sea daffodil extract was purchased from Shaanxi Xinkang Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0130] The preservative is a combination of p-hydroxyacetophenone and phenoxyethanol.

[0131] p-Hydroxyacetophenone is commercially available, CAS number: 99-93-4.

[0132] Phenoxyethanol is commercially available, CAS number: 122-99-6.

[0133] The preparation method of the solvent solution comprises the following steps:

[0134] Step 01), add 88.6838 kg of deionized water into an emulsifying pot and heat to 90°C, keep at 90°C, rotate at 120 r / min, stir for 20 min, and cool down.

[0135] Step 02), deionized water was cooled to 60°C, 0.5kg p-hydroxyacetophenone, 0.0606kg phenoxyethanol, 0.09kg 1,2-pentanediol, 5.15365kg butanediol, 0.544kg 1,2-hexanediol were added, the speed was 120r / min, stirred for 5min, cooled to 45°C, 2.55kg hydroxyethylpiperazineethanesulfonic acid, 0.55kg carnosine, 0.575kg arginine were added. PCA, 0.0045kg tetrapeptide-1, 0.00045kg nonapeptide-1, 0.003kg Kava Piper leaf / root / stem extract, 0.26125kg madecassoside, 0.01375kg Centella asiatica extract, 0.505kg red algae extract, 0.505kg sea daffodil extract, speed 120r / min, stirring for 20min, filter the material with 500 mesh filter cloth to obtain a solvent solution.

[0136] Example 3

[0137] A double-antibody brightening freeze-dried essence comprises freeze-dried powder and a solvent liquid. The mass of the freeze-dried powder in each dose of the double-antibody brightening freeze-dried essence is 130 mg, and the volume of the solvent liquid is 3 ml.

[0138] The lyophilized powder is prepared by mixing and lyophilizing the following components:

[0139] Solvent, Skin Conditioner, Antioxidant.

[0140] The solvent is deionized water.

[0141] The skin conditioning agent is a compound of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch glycolate octenylsuccinate and silica.

[0142] Mannitol was commercially available, CAS number: 69-65-8.

[0143] Trehalose is commercially available, CAS number: 99-20-7.

[0144] The Haematococcus pluvialis extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0145] Sodium starch octenylsuccinate is commercially available, CAS number: 66829-29-6.

[0146] Silica was purchased from Evonik in Germany, model: SIPERNAT 2200PC.

[0147] The antioxidant is a compound of sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid.

[0148] Sodium ascorbate is commercially available, CAS number: 134-03-2.

[0149] Ascorbic acid was commercially available, CAS number: 299-36-5.

[0150] 3-o-Ethyl ascorbic acid was commercially available, CAS No.: 86404-04-8.

[0151] The preparation method of freeze-dried powder comprises the following steps:

[0152] Step 1), 78kg of deionized water, 10kg of mannitol and 2kg of trehalose are mixed and heated to 85°C, kept at 85°C, rotated at 120r / min, stirred for 30min, cooled to 45°C, 0.27kg of Haematococcus pluvialis extract, 0.679kg of sodium octenyl succinate starch, 0.025kg of sodium ascorbate and 0.006kg of silica are added, rotated at 120r / min, stirred for 5min, 8kg of 3-o-ethyl ascorbic acid and 1.02kg of ascorbic acid are added, rotated at 120r / min, stirred for 5min, and the material is filtered with a 500-mesh filter cloth to obtain a mixture.

[0153] Step 2), freeze-drying the mixture at -55°C to obtain freeze-dried powder.

[0154] The solvent liquid consists of the following components:

[0155] Solvent, anti-glycation agent, moisturizer, whitening composition, preservative.

[0156] The solvent is deionized water.

[0157] The anti-glycation agent is a compound of carnosine, arginine PCA, tetrapeptide-1, and nonapeptide-1.

[0158] Carnosine was commercially available, CAS number: 305-84-0.

[0159] Arginine PCA was commercially available, CAS number: 56265-06-6.

[0160] Tetrapeptide-1 is commercially available, CAS number: 1632354-04-1.

[0161] Nonapeptide-1 was commercially available, CAS number: 158563-45-2.

[0162] The moisturizing agent is a compound of 1,2-pentanediol, butylene glycol, 1,2-hexanediol and hydroxyethylpiperazineethane sulfonic acid.

[0163] 1,2-Pentanediol is commercially available, CAS number: 5343-92-0.

[0164] Butanediol is commercially available, CAS number: 110-63-4.

[0165] 1,2-Hexanediol is commercially available, CAS number: 6920-22-5.

[0166] Hydroxyethylpiperazineethanesulfonic acid is commercially available, CAS number: 7365-45-9.

[0167] The whitening composition is a compound of Kava Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract and Sea Narcissus extract.

[0168] Kava leaf / root / stem extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0169] Madecassoside is commercially available, CAS number: 34540-22-2.

[0170] Centella asiatica extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0171] The red algae extract was purchased from Guangzhou Ruiran Biotechnology Co., Ltd.

[0172] The sea daffodil extract was purchased from Shaanxi Xinkang Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0173] The preservative is a combination of p-hydroxyacetophenone and phenoxyethanol.

[0174] p-Hydroxyacetophenone is commercially available, CAS number: 99-93-4.

[0175] Phenoxyethanol is commercially available, CAS number: 122-99-6.

[0176] The preparation method of the solvent solution comprises the following steps:

[0177] Step 01), add 81.5502 kg of deionized water into an emulsifying pot and heat to 90°C, keep at 90°C, rotate at 120 r / min, stir for 20 min, and cool down.

[0178] Step 02), deionized water is cooled to 60°C, 0.6kg p-hydroxyacetophenone, 0.12kg phenoxyethanol, 0.16kg 1,2-pentanediol, 7.3782kg butanediol, 0.6788kg 1,2-hexanediol are added, the speed is 120r / min, stirred for 5min, cooled to 45°C, 5kg hydroxyethylpiperazine ethane sulfonic acid, 1kg carnosine, 1kg arginine PCA, 0.008kg tetrapeptide-1, 0.0008kg nonapeptide-1, 0.004kg kava pepper leaf / root / stem extract, 0.475kg madecassoside, 0.025kg Centella asiatica extract, 1kg red algae extract, 1kg sea daffodil extract, the speed is 120r / min, stirred for 20min, filtered with a 500-mesh filter cloth to obtain a solvent solution.

[0179] Comparative Example 1

[0180] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0181] Replace the Kava leaf / root / stem extract in equal amounts with Glycyrrhiza glabra extract.

[0182] The extract of Glycyrrhiza glabra was purchased from Shaanxi Tianshengyuan Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.

[0183] Comparative Example 2

[0184] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0185] Madecassoside was replaced with lavender extract in equal amounts.

[0186] Lavender extract was purchased from Shaanxi New Horizon Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0187] Comparative Example 3

[0188] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0189] An equal amount of pomegranate peel extract was used to replace the Centella asiatica extract.

[0190] Pomegranate peel extract was purchased from Shaanxi Bolin Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0191] Comparative Example 4

[0192] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0193] Camu camu extract was used to replace the red algae extract in an equal amount.

[0194] Camu camu extract was purchased from Shaanxi Lvlai Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0195] Comparative Example 5

[0196] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0197] An equal amount of Portulaca oleracea extract was used to replace the Sea Narcissus extract.

[0198] Purslane extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0199] Comparative Example 6

[0200] A dual-antibiotic brightening freeze-dried essence, compared with Example 2, the only difference is:

[0201] An equal amount of Kava Piper mesona leaf / root / stem extract was used to replace Glycyrrhiza glabra extract, an equal amount of Madecassoside was used to replace Lavender extract, an equal amount of Centella asiatica extract was used to replace Pomegranate peel extract, an equal amount of Rhododendron camu extract was used to replace Red Algae extract, and an equal amount of Portulaca oleracea extract was used to replace Sea Narcissus extract.

[0202] The extract of Glycyrrhiza glabra was purchased from Shaanxi Tianshengyuan Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.

[0203] Lavender extract was purchased from Shaanxi New Horizon Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0204] Pomegranate peel extract was purchased from Shaanxi Bolin Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0205] Camu camu extract was purchased from Shaanxi Lvlai Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0206] Purslane extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.

[0207] Experiment 1

[0208] Several volunteers, aged 30-35 years old, female, with an initial melanin content of 300-400 on their facial skin, were recruited.

[0209] Ten volunteers formed a group and were divided into two groups to use the double-antibody brightening freeze-dried essence of each embodiment and comparative example.

[0210] The volunteers used the double-anti-brightening freeze-dried essence once every night. Before use, they first washed their face with clean water and a disposable face towel, wiped it dry with a clean towel, and then took a portion of the double-anti-brightening freeze-dried essence and applied it evenly on the face.

[0211] When recruiting volunteers, wash your face with clean water and a disposable face towel, dry it with a clean towel, and then use the MX18 melanin tester from the German CK company to test the melanin content and record it. The detection point is the middle of the forehead, which is recorded as the initial melanin content. If the initial melanin content is not between 300-400, you cannot participate in the experiment.

[0212] After using it continuously for 7 days and 28 days respectively, the melanin content was tested again in the morning of the next day (i.e. the morning of the 8th day and the 29th day). Before testing, clean the face with clean water and a disposable face washcloth, wipe it dry with a clean towel, and then use the MX18 melanin tester of the German CK company to test the melanin content and record it. The testing point is the middle of the forehead, and the melanin content is recorded as the whitening 7 days and 28 days.

[0213] During the experiment, volunteers participated in outdoor activities for 2 hours every day. The experiment was conducted in summer and during sunny periods.

[0214] The mean value of each group of experimental data was taken.

[0215] The specific test data of Experiment 1 are shown in Table 1.

[0216] Table 1

[0217]

[0218]

[0219] According to the data comparison of each embodiment and comparative example in Table 1, it can be obtained that after 7 days of use, the melanin content of each embodiment decreases significantly, while the melanin content of each comparative example decreases at a slower rate. The decrease in the melanin content of each embodiment after 7 days of use is greater than the decrease in the melanin content of each comparative example after 28 days of use. It can be seen that even if each comparative example uses an extract with whitening effect, it is difficult to penetrate into the deep layer of the skin due to the inability to open the skin channel, resulting in a general effect of inhibiting tyrosinase and then inhibiting melanin synthesis, resulting in a slow whitening effect. When the compound of Kava Piper melanin leaf / root / stem extract, hydroxy-madecassoside, Centella asiatica extract, red algae extract, and sea daffodil extract is used, it can promote the skin's absorption of active ingredients, so that the active ingredients reach the deep skin, and then quickly play a significant effect of inhibiting melanin synthesis. In combination with the antioxidant and anti-glycation effects, the skin quickly regains its youthfulness, thereby accelerating melanin metabolism and making the skin quickly become fair and shiny.

[0220] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A dual-antibiotic brightening freeze-dried essence, characterized in that: It comprises a lyophilized powder and a solvent liquid, wherein the ratio of the mass of the lyophilized powder to the volume of the solvent liquid is 100-130 mg: 2-3 ml; The freeze-dried powder is prepared by mixing the following components by mass and then freeze-drying: Solvent 78-91.4499 parts; Skin conditioning agent 5.54775-12.955 parts; Antioxidant 3.00235-9.045 parts; The solvent liquid is composed of the following components in parts by mass: Solvent 81.5502-95.8174 parts; Anti-glycation agent 0.2511-2.0088 parts; Moisturizer 3.4583-13.217 parts; 0.072-2.504 parts of whitening composition; Preservative 0.4012-0.72 parts; The whitening composition is a compound of Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract, and Sea Narcissus extract; The mass ratio of the Piper melongena leaf / root / stem extract, madecassoside, Centella asiatica extract, Rhodophyta algae extract, and Narcissus salsa extract is 0.002-0.004: 0.0475-0.475: 0.0025-0.025: 0.01-1: 0.01-1; In the freeze-dried powder, the antioxidant is a compound of sodium ascorbate, ascorbic acid, and 3-o-ethyl ascorbic acid; In the solvent solution, the anti-glycation agent is a compound of carnosine, arginine PCA, tetrapeptide-1 and nonapeptide-1.

2. The dual-antibiotic brightening freeze-dried essence according to claim 1, characterized in that: The mass ratio of the sodium ascorbate, ascorbic acid and 3-o-ethyl ascorbic acid is 0.00125-0.025:0.0011-1.02:3-8.

3. The dual-antibiotic brightening freeze-dried essence according to claim 1, characterized in that: The mass ratio of carnosine, arginine PCA, tetrapeptide-1 and nonapeptide-1 is 0.1-1: 0.15-1: 0.001-0.008: 0.0001-0.0008.

4. The dual-antibiotic brightening freeze-dried essence according to claim 1, characterized in that: In the freeze-dried powder, the skin conditioning agent is a compound of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch glycolate octenylsuccinate, and silica; In the solvent liquid, the humectant is a compound of 1,2-pentanediol, butanediol, 1,2-hexanediol, and hydroxyethylpiperazineethane sulfonic acid; In the solvent liquid, the preservative is a compound of p-hydroxyacetophenone and phenoxyethanol.

5. The dual-antibiotic brightening freeze-dried essence according to claim 4, characterized in that: The mass ratio of mannitol, trehalose, Haematococcus pluvialis extract, sodium starch octenylsuccinate and silica is 5-10: 0.5-2: 0.0135-0.27: 0.03395-0.679: 0.0003-0.006; The mass ratio of 1,2-pentanediol, butanediol, 1,2-hexanediol and hydroxyethylpiperazineethane sulfonic acid is 0.02-0.16: 2.9291-7.3782: 0.4092-0.6788: 0.1-5; The mass ratio of p-hydroxyacetophenone to phenoxyethanol is 0.4-0.6:0.0012-0.

12.

6. A method for preparing the dual-antibody brightening freeze-dried essence according to any one of claims 1 to 5, characterized in that: Including freeze-dried powder preparation method and solvent liquid preparation method; The preparation method of the freeze-dried powder comprises the following steps: Step 1), mixing the components uniformly to obtain a mixture; Step 2), freeze-drying the mixture at (-45)-(-55)°C to obtain freeze-dried powder; The preparation method of the solvent solution comprises the following steps: Step 01), heating the solvent for sterilization; Step 02), add the remaining components into the solvent, mix evenly, and obtain a solvent solution.

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