A Chinese veterinary medicine composition for treating or / and preventing chicken synoviae mycoplasma disease and its preparation method and use

Abstract

The present invention provides a Chinese veterinary medicine composition for treating or / and preventing chicken synovial mycoplasma disease, and its preparation method and use, which belong to the field of Chinese veterinary medicine. The present invention provides a Chinese veterinary medicine composition for treating or / and preventing chicken synovial mycoplasma disease, which is prepared from the following raw materials in weight ratio: 40-60 parts of Sichuan Cyathula, 20-40 parts of Cinnamon Bark, 50-70 parts of Stephania Tetrandrae, 40-60 parts of Ephedra, 50-70 parts of Gentiana Macrophylla, and 20-40 parts of Licorice. The present invention also provides a preparation method and use of the Chinese veterinary medicine composition. The present invention is a pure Chinese medicine preparation, which is green, safe, and has no residue. Through experiments conducted in chicken farms, the results show that the present invention is applicable to both the treatment and prevention stages, can effectively reduce the incidence of chicken synovial mycoplasma disease, improve symptoms such as chicken joint swelling and emaciation, and each raw material is used in combination to play a synergistic effect, significantly reduce the cost of medication, and provide a new choice for Chinese veterinary medicine to treat chicken synovial mycoplasma disease.

Description

Technical Field

[0001] The invention relates to a Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease, belonging to the field of Chinese veterinary medicine. Background Art

[0002] Chicken synovial mycoplasma disease, also known as infectious synovitis, is an acute to chronic disease in chickens and turkeys. Clinically, it mainly harms the synovial bursa and tendon sheath of the joints, causing exudative inflammation of the synovial bursa membrane and tendon sheath synovium of the joints. The transmission routes include horizontal transmission, vertical transmission and other routes. It can be infected at different ages, and the younger the age, the stronger the susceptibility. Acute infection is usually seen in chickens aged 4 to 16 weeks and turkeys aged 10 to 24 weeks, and chronic infection can be seen at any age. It occurs all year round, and is most likely to occur in winter and spring, and it is increasing year by year. The initial symptoms are pale combs, lameness and slow growth. As the disease progresses, the feathers become rough and messy, the combs shrink, the spirit becomes depressed, and the weight is lost. The characteristic lesions mainly manifest as swollen foot pads, swollen tarsal joints, and keel cysts. However, as the body recovers, symptoms such as spirit will slowly disappear, and characteristic lesions in joints and other parts will always exist. In the early autopsy of the swollen part, there is a small amount of white or light yellow transparent exudative fluid after dissection; as the disease progresses, the secretion increases and becomes turbid or yellow cheesy. During this period, the surface of the joints, especially the surface of the tarsal joints and shoulder joints, becomes thinner to varying degrees, forming depressions. The incidence rate in the chicken flock is high and shows an increasing trend, which makes them in a sub-healthy state for a long time. After infection, it is easy to affect growth and development, resulting in delayed production and no peak egg production. It has a serious impact on the growth and production of the chicken flock and causes huge economic losses to the farm. The main sources of infection are sick chickens and latently infected chickens. Food sources, feeding utensils, air, etc. contaminated by pathogens are also potential sources of infection. Various stresses, mycotoxin poisoning, improper feeding management (high density, poor ventilation) and other factors are important causes of the disease. This disease not only affects the growth performance and egg production rate of chickens, but may also lead to mixed infections with avian influenza, Newcastle disease virus and Escherichia coli, increase the mortality rate of chicken flocks, and cause serious economic losses to the poultry farming industry.

[0003] Clinical prevention is mainly based on vaccination, and commonly used drugs for treatment include tylosin, tylosin, enrofloxacin, lincomycin, etc., but the incidence and recurrence rates are still very high. In addition, antibiotic treatment is prone to drug resistance, which has a certain impact on the health of chickens and humans. At the same time, it may produce certain toxic side effects, causing damage to the liver and kidneys of chickens, and affecting the growth and development of chickens. Due to the disadvantages of antibacterial drugs and the market demand for this disease, green and antibiotic-free Chinese veterinary medicine products have become an option.

[0004] Patent application number CN202111251980.9, invention name: Chinese medicinal compound preparation for treating chicken synoviae mycoplasma disease, targeting the pathogenic characteristics of chicken synoviae mycoplasma, combined with the theory of traditional Chinese medicine, a Chinese medicinal compound preparation for treating chicken synoviae mycoplasma disease can play a therapeutic role in chicken synoviae mycoplasma. The invention is made of effective ingredients: Scutellaria baicalensis, Smilax glabra, Stephania tetrandra, and Cyathula obtusifolia. The Chinese medicinal compound preparation of the invention can significantly improve the symptoms of joint swelling and has a significant therapeutic effect on chicken synoviae mycoplasma disease. Evaluation of Efficacy of Compound Chinese Medicinal Herbsagainst Mycoplasma synoviae using different Lab Tests in Mouse and Chicken, Atif Rehman et al., Intl. J. Agric. Biol., pp. 1-7, April 2019, disclosed: The inhibition zone of Cyathula obtusifolia against chicken synoviae mycoplasma is 21.17+0.89mm, and the sensitivity is medium.

[0005] According to the syndrome differentiation of traditional Chinese veterinary medicine, chicken synovial mycoplasma disease belongs to dampness and arthralgia. "Arthralgia" refers to obstruction and blockage. The causes of arthralgia are mostly due to insufficient yang energy and weak defensive qi in the animal body. In addition, when the climate changes suddenly and the patient lies in wetlands for a long time, the wind, cold and dampness will take advantage of the situation to enter, injure the skin, flow through the meridians, and invade the muscles, joints, tendons and bones, resulting in meridian blockage and stagnation of qi and blood. This will then cause joint muscle swelling and pain, poor flexion and extension, and even numbness. In severe cases, there will also be symptoms such as joint swelling and deformation, and even inability to lie on the ground. In the clinical treatment of this disease, attention should be paid to dispelling wind and cold, relaxing muscles and activating blood circulation, and removing dampness and unblocking meridians. It has been reported that the use of Qinling Decoction (composed of Scutellaria baicalensis, Smilax glabra and Cyathula officinalis) has a good therapeutic effect on chicken synovial mycoplasma disease (Zhang Xiaoning, Study on the Therapeutic Effect of Qinling Decoction on Chicken Synovial Mycoplasma Disease, Master's Thesis of Jilin University, May 2022).

[0006] The traditional Chinese veterinary medicine program for treating chicken synovia bursa mycoplasma disease is usually based on the principle of TCM syndrome differentiation and treatment. Different traditional Chinese medicine formulas are selected according to the specific symptoms and constitution of the sick chickens, but it still cannot meet the market demand. Summary of the invention

[0007] The technical scheme of the present invention is to provide a Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease. Another technical scheme of the present invention is to provide a preparation method and use of the Chinese veterinary medicine composition.

[0008] The present invention provides a Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease, which is prepared from the following raw materials in weight proportion:

[0009] 40-60 parts of Cyathula, 20-40 parts of Cinnamon Bark, 50-70 parts of Stephania Tetrandrae, 40-60 parts of Ephedra, 50-70 parts of Gentiana Macrophylla, and 20-40 parts of Licorice.

[0010] Preferably, it is prepared from the following bulk drugs in the following weight ratio:

[0011] 50 parts of Cyathula officinalis, 30 parts of Cortex Cinnamomi, 60 parts of Radix Tetrandrae, 50 parts of Ephedra, 60 parts of Radix Gentianae Macrophyllae, and 30 parts of Radix Glycyrrhizae.

[0012] The veterinary drug composition of the present invention is prepared from the raw drug powder, water or organic solvent extract of the raw drug as active ingredients, and pharmaceutically acceptable excipients or auxiliary ingredients are added to form a commonly used pharmaceutical preparation.

[0013] Wherein, the preparation is a mixture, powder, granule, tablet or pill.

[0014] The present invention provides a method for preparing the Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease, which comprises the following steps:

[0015] a. Weigh the APIs of various weight ratios;

[0016] b. The raw medicine is ground into powder, or boiled in water for extraction, and then pharmaceutically acceptable excipients or auxiliary ingredients are added to prepare a commonly used pharmaceutical preparation.

[0017] The invention provides use of the Chinese veterinary medicine composition in preparing medicine for treating or / and preventing chicken synoviae mycoplasma disease.

[0018] The principle of the prescription for treating and / or preventing chicken synoviae mycoplasma disease of the present invention is as follows:

[0019] Sichuan Cyathula: Also known as Baibei (Shennong's Herbal Classic), it is a plant of the Amaranthaceae family. Cyathula officinalis Kuan The dried root of the plant. It tastes sweet and slightly bitter, and is neutral in nature. It enters the liver and kidney meridians. Efficacy: removes blood stasis and promotes menstruation, promotes joint function, and promotes diuresis and promotes blood circulation. Indications: amenorrhea, abdominal masses, retained placenta, joint pain, foot paralysis and muscle spasm, hematuria and bloody stranguria, and injuries from falls. "Yanggeng Ji" says: "It promotes ventilation and moves the feet."

[0020] Cinnamon: Also known as cinnamon heart (from "Fan Mu Zhuang Yan Fang"), it is a plant of the Lauraceae family, cinnamon Cinnamomum cassia The dried bark of Presl. It tastes spicy and sweet, and is very hot. It enters the kidney, spleen, heart, and liver meridians. Efficacy: replenishing fire and yang, guiding fire back to the origin, dispersing cold and relieving pain, warming and unblocking the meridians. Indications: cold pain in the waist and knees, abdominal pain caused by cold hernia, floating of false yang, vomiting and diarrhea caused by deficiency cold, etc. "Veterinary Medicinal Properties" says: "Guide fire back to the origin, and treat cold pain in animals."

[0021] Stephania tetrandra: also known as stone solution (Compendium of Materia Medica), is a plant of the Menispermaceae family. Stephania Tertrandra The dried root of S.Moore. Bitter in taste, cold in nature. Enters the bladder and lung meridians. Efficacy: dispel wind and relieve pain, promote diuresis and reduce swelling. Indications: rheumatic arthralgia, edema, beriberi, urinary incontinence, etc. "Common Chinese Herbal Medicines for Veterinary Medicine" says: "It dispels wind and promotes diuresis, promotes qi and relieves pain, and activates blood circulation and disperses blood stasis."

[0022] Ephedra: Also known as horse hair yellow medicine (Veterinary Materia Medica), it is a plant of the Ephedraceae family. Ephedra sinica Stapf, Ephedra sinica Ephedra intermedia Schrenk et CA Mey. or Equisetum arvense Ephedra Equisetina The dried herbaceous stem of Bge. It is pungent, slightly bitter, and warm in nature. It enters the lung and bladder meridians. Efficacy: sweating and dispelling cold, clearing the lungs and relieving asthma, promoting diuresis and reducing swelling. Indications: colds caused by wind and cold, chest tightness, asthma and cough, edema caused by wind and water, etc. "Qiu Ji Tong Xuan Lun" says: "It can treat all kinds of wind and relieve the exterior symptoms, and it can be used to remove the veil."

[0023] Qinjiao: also known as Qinjiao (Annotations to the Classic of Materia Medica), a plant of the Gentianaceae family Gentiana macrophylla Pall., Gentiana macrophylla Gentiana straminea Maxim., Gentiana macrophylla Gentiana crassicaulis Duthie exBurk. or Gentiana macrophylla Gentiana dahurica The dried root of Fisch. It tastes pungent and bitter, and is neutral in nature. It enters the stomach, liver, and gallbladder meridians. Efficacy: dispel rheumatism, clear damp-heat, stop pain, and relieve deficiency fever. Indications: rheumatic pain, tendon spasm, joint pain, bone steaming and hot flashes, etc. "Yang Geng Ji" says: "It removes dampness and heat from the twelve meridians and regulates the tendons and bones."

[0024] Licorice: Also known as sweet root (Chinese Materia Medica), it is a plant of the leguminous plant Licorice. Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Glycyrrhiza inflata Bat. or Glycyrrhiza glabra Glycyrrhiza glabra The dried root and rhizome of L. It is sweet and neutral in nature. It enters the heart, lung, spleen and stomach meridians. Efficacy: clearing away heat and detoxifying, relieving pain, and harmonizing various medicines. Indications: acute pain in the abdomen and limbs, carbuncle, ulcer, and relieving drug toxicity and potency. "Shi Huang An Ji Collection" says: "It stops chest pain and cough, opens the hair orifices, warms the internal organs, and regulates blood vessels."

[0025] Chickens infected with mycoplasma synoviae are characterized by lack of energy, swelling of the tarsal joints, lying down, decreased appetite, stagnation of growth, and weight loss. Typical symptoms are swelling and lameness of the tarsal and metatarsal joints, even deformation, sternal cysts, tenosynovitis and osteoarthritis. The present invention judges that the disease is caused by the body's weak defensive qi according to the above symptoms, and is susceptible to wind and cold, and then there is runny nose and fear of cold; if the spleen and kidney yang are deficient and the middle yang is insufficient, then there is fear of cold, loss of appetite, and indigestion; when the wind, cold and dampness evil enters the multi-viscera stage from the surface to the inside, then there is joint swelling and edema of the joint capsule; if the wind, cold and dampness evil blocks the meridians and stagnates for a long time, then the heat is transformed into damage to the yin, then the cold and heat are mixed, and the liver and kidney are not nourished, and the tendons and bones are not nourished, then the joints are not flexed and extended, and there is pain.

[0026] It can be seen that this disease belongs to the category of Bi syndrome, which is a syndrome of phlegm and blood stasis, and is related to cold evil, dampness evil, heat evil, etc. Bi means closure, which refers to the blockage of meridians, stagnation of Yingwei, and poor circulation of qi and blood in the internal organs. The Yellow Emperor's Internal Classic points out that "the three qi of wind, cold and dampness are mixed together to form Bi"; the Bi theory chapter of the Internal Classic says that "in spring, tendon Bi occurs, in summer, pulse Bi occurs, in the long summer, muscle Bi occurs, in autumn, skin Bi occurs, and in winter, bone Bi occurs." If it does not heal for a long time, then "all the five internal organs have their own combination, and if the disease does not go away for a long time, it will stay in the combination." The pathogenic factors repeatedly invade the body, from the skin surface to the muscles and then to the tendons and bones. The condition does not heal for a long time, making it a stubborn Bi.

[0027] Under the guidance of TCM theory, the key points of syndrome differentiation are: arthritis lasts for a long time, joint and muscle pain is severe, the pain is fixed, joints are deformed and swollen, and functional disorders occur. Swelling is caused by meridian blockage and stagnation of qi and blood, and pain is caused by stagnation of qi and blood. The treatment is based on the theory of "pain if it is blocked, and pain if it is unblocked" and "treating wind starts with treating blood, and wind will disappear when blood circulates".

[0028] Compared with the "Qinling Decoction" published in May 2022 by Zhang Xiaoning, "Study on the therapeutic effect of Qinling Decoction on chicken synovial bursal mycoplasma disease", a master's thesis of Jilin University, the present invention uses Sichuan Cyathula as the main medicine during treatment to exert its effects of promoting blood circulation and unblocking collaterals, removing rheumatism, and benefiting joints, that is, targeting the main symptoms of swollen joints and edema of the joint capsule of chickens, while Qinling Decoction uses Scutellaria baicalensis as the main medicine, which is bitter, cold, and dry, and mainly clears heat and dries dampness to eliminate arthritis symptoms caused by dampness. The present invention uses cinnamon bark and ephedra as ministerial medicines, one of which replenishes fire and assists yang, unclogs blood vessels, and the other dispels wind and cold, promotes diuresis and reduces swelling. The two work together to strengthen the effect of Sichuan Cyathula, and Qinling Decoction uses Smilax glabra as the ministerial medicine, which promotes diuresis and dries dampness, and dredges joints, and still mainly eliminates dampness. The combination of the adjuvant drugs of the present invention, namely, the Chinese Gentiana macrophylla and the Stephania tetrandra, can not only enhance the effect of dredging collaterals and relieving pain, but also can respond to the symptoms of bone steaming and hot flashes caused by the stagnation of heat in the arthralgia. Licorice can not only harmonize the various drugs, but also tonify the middle and replenish qi as an adjuvant drug. The Qinling Decoction uses the Rhizoma Cynoglossi as an auxiliary adjuvant drug, and cooperates with the main drug to enhance its effect of dredging joints.

[0029] Combined with the course of the disease of chicken synovial bursa mycoplasma disease, the disease belongs to arthralgia according to Chinese veterinary dialectics, which is caused by the body's weak defensive qi, and the body is susceptible to wind and cold, which will cause cold runny nose; if the spleen and kidney yang are deficient and the middle yang is insufficient, the patient will be afraid of cold, have poor appetite and indigestion; when the wind, cold and dampness are felt from the surface to the inside, and enter the multi-viscera stage, the patient will see joint swelling and joint capsule edema; if the wind, cold and dampness evil block the meridians and stagnate for a long time, the heat will damage the yin, and the cold and heat will be mixed, and the liver and kidney will be malnourished, and the tendons and bones will be malnourished, and the joints will be unfavorable and painful. The prescription of the present invention can remove dampness and dredge the meridians, dispel wind and dissipate cold, and can also activate blood circulation and remove blood stasis, reduce swelling and relieve pain. All medicines are used together, and the effects of clearing heat and removing dampness, warming the middle and dispersing cold, removing arthralgia and relieving pain are played together. Qinling Decoction focuses on dampness evil, mainly clearing heat and drying dampness, and promoting joint function, and is mainly used to treat damp arthralgia.

[0030] The Yellow Emperor's Classic of Internal Medicine points out that "the three elements of wind, cold and dampness combine to form arthralgia". If arthralgia does not heal for a long time, it will spread to the interior. Therefore, there is a saying that "the five internal organs are all combined, and if the disease does not go away for a long time, it will stay in the combination." The wind, cold and dampness evil of the sick chicken spreads from the surface to the interior. As the pathogenic evil penetrates deeper, the condition becomes increasingly complicated. If the arthralgia blocks the meridians for a long time, it will turn into heat and damage the yin, and then cold and heat will be mixed. In terms of the change of the location of the disease, because the disease did not heal the previous time and was infected again, it penetrated from the outside to the inside, from the skin surface to the muscles, and then from the muscles to the tendons and bones. It is consistent with the process of syndrome differentiation and transmission in the Six-Jing Shanghan Lun, which is the process of the external evil from Taiyang syndrome to Yangming or Shaoyang, then to Taiyin, and then to Shaoyin Jueyin. The symptoms of mycoplasma synoviae disease in chickens include lethargy, lying down, decreased appetite, stagnation of growth, weight loss, swelling and deformation of tarsal and metatarsal joints, lameness, and in the later stage, nodules or cysts begin to appear on the chest, and tenosynovitis and osteoarthritis characterized by yellow watery or viscous or cheesy exudates in the joint cavity. The condition is complicated and cannot be simply attributed to rheumatism.

[0031] The efficacy and explanation of the prescription for treating chicken synoviae mycoplasma disease of the present invention are as follows:

[0032] Efficacy: Removes dampness and dredges meridians, dispels wind and cold, promotes blood circulation and removes blood stasis, reduces swelling and relieves pain.

[0033] Indications: Arthritis caused by wind-cold, dampness-heat obstruction, with symptoms of chills, runny nose, swollen, stiff and deformed joints, and difficulty in flexion and extension.

[0034] Explanation of the prescription: In the prescription, Rhizoma Cynanchum is the main drug, which is good at promoting blood circulation and unblocking collaterals, removing rheumatism, and benefiting joints; Rhizoma Cinnamomi is mild in nature, can guide fire back to the origin, and is good at dispelling cold and relieving pain, replenishing fire and supporting Yang, and has the function of unblocking blood vessels; Ephedra promotes the Yang of the Wei, dredges the meridians to drive away the wind and cold from the outside, and disperses the evil from the surface. The two are the assistant drugs, warming and unblocking the three burners, removing the cold evil in the Rong, relieving the wind and heat in the Wei, and replenishing the deficiency of the lower burner to unblock the nine orifices and benefit the joints; Gentiana macrophylla is moist but not dry, and is the wind medicine. As a moisturizing agent, it is good at guiding dampness and heat downward, and is suitable for rheumatism and arthralgia, whether it is new or long-standing. When used together with ephedra and cinnamon, it can eliminate wind-cold dampness and arthralgia. Because of its bitter and cold nature, it can clear away heat and promote diuresis, and is good at moving downward to relieve dampness and heat in the lower bladder. The two herbs together can enhance the effect of dredging meridians and relieving pain, and eliminate the symptoms of bone steaming and hot flashes caused by long-term arthralgia in the tendons, bones and joints, and the stagnation and heat. Licorice is a guiding drug, which can replenish the middle qi, harmonize the middle and relieve urgency, detoxify, and harmonize the properties of the drugs. The combination of these herbs can clear away heat and dampness, warm the middle and dispel cold, eliminate arthralgia and relieve pain, and treat both cold and heat, and play the effect of removing dampness and dredging meridians.

[0035] The beneficial effects of the present invention are:

[0036] The invention is a pure Chinese medicine preparation, which is green, safe and has no residue. The results of the test conducted in the chicken farm show that the invention is applicable in both the treatment and prevention stages, can effectively reduce the incidence of chicken synoviae mycoplasma disease, and improve the symptoms of chicken joint swelling, emaciation and the like. When in use, 500 ml of the Chinese medicine compound liquid is diluted with 100 jin of water, and the chickens are concentrated for drinking. The total effective rate of continuous use for 5 to 7 days is above 85%. The raw materials are used in combination to exert a synergistic effect, significantly reduce the cost of medication, and are safer to use, thereby providing a new choice for Chinese veterinary medicines to treat chicken synoviae mycoplasma disease. DETAILED DESCRIPTION

[0037] Example 1 Preparation of the veterinary drug mixture of the present invention

[0038] Weigh the raw materials: 40 grams of Cyathula capitata, 20 grams of cinnamon bark, 50 grams of Stephania tetrandra, 40 grams of ephedra, 50 grams of Gentiana macrophylla, and 20 grams of liquorice, add water to boil, filter, and then add water to prepare 2500 ml of medicinal solution.

[0039] Example 2 Preparation of the veterinary drug mixture of the present invention

[0040] Weigh the raw materials: 50 grams of Cyathula officinalis, 30 grams of cinnamon bark, 60 grams of Stephania tetrandra, 50 grams of ephedra, 60 grams of Gentiana macrophylla, and 30 grams of liquorice, and prepare according to the method of Example 1.

[0041] Example 3 Preparation of the veterinary drug mixture of the present invention

[0042] Weigh the raw materials: 60 grams of Cyathula officinalis, 40 grams of cinnamon bark, 70 grams of Stephania tetrandra, 60 grams of Ephedra, 70 grams of Gentiana macrophylla, and 40 grams of Licorice, and prepare according to the method of Example 1.

[0043] Usage: Add 500ml to 500kg of water, and drink it within 4-6 hours based on the total daily intake. One course of treatment is 5-7 days.

[0044] The beneficial effects of the present invention are demonstrated by pharmacodynamic tests as follows.

[0045] Test Example 1 Test on the efficacy of the veterinary medicine of the present invention in treating chicken synoviae mycoplasma disease

[0046] Inclusion criteria for suspected cases of Mycoplasma synoviae:

[0047] 1.1 Clinical symptoms: Poor mental state, pale and atrophied combs, no or little eating, messy feathers, swollen joints, prone to lying down, and emaciated chickens. The egg production rate of laying hens decreases, and the tip of some eggshells is deformed.

[0048] 1.2 Clinical anatomy: Autopsy can reveal obvious yellow gelatinous substance in the synovial bursa, effusion in the tarsal and toe joints, or air sac inflammation due to respiratory infection.

[0049] 1.3 Chinese medicine syndrome differentiation: Suspected chickens grow slowly, have messy feathers, limp, unstable gait, pale combs, like to lie down, and are not sociable. The joints are swollen and there is a sense of fluctuation when touched. The symptoms vary in different stages of the disease, such as severe swelling and protrusion of joints, abnormal thickening of foot pads, etc. Based on the above symptoms, the disease is judged to be spleen yang deficiency, liver and kidney deficiency, and the three evils of wind, cold and dampness infiltrating the body, resulting in wind-cold-dampness arthralgia syndrome.

[0050] 1.4 Indirect ELISA

[0051] The operation and judgment were carried out according to 5.4 of NY / T4035-2021, and those with positive results were included as cases.

[0052] In summary, those who meet the above conditions can be included in the experimental group.

[0053] 2. Treatment criteria

[0054] ①Clinical symptom evaluation criteria

[0055] score Daily Activities Mental state Eating and drinking water cure normal normal normal efficient Slight limp, abnormal standing posture, not moving, preferring to lie on the ground Depressed, disheveled feathers, pale comb, half-closed eyes Not actively eating or drinking invalid Severe limp, slow movement, and recumbency Mental depression and slow response to external factors Loss of appetite, severe weight loss

[0056] ② Criteria for judging autopsy symptoms

[0057]

[0058] Preparation and Usage of the Medicinal Solution: The Chinese medicine in the embodiment is prepared in the following manner, each raw material is fully mixed, and water is added to decoct and extract to prepare 2500 ml of medicinal solution. Each 500 ml is diluted with 500 jin of water, and it is calculated as the full-day amount and consumed in 4-6 hours. A course of treatment is 5-7 days.

[0059] 3. Test content

[0060] In a chicken farm in Leshan, Sichuan, 10,000 white-feathered broilers, aged 26 days, were confirmed to be infected with Mycoplasma synoviae through clinical symptoms, pathological examination, and indirect ELISA. The disease was found in every building, with an overall incidence of about 14%.

[0061] 880 sick chickens were selected for the test and randomly divided into 8 groups, each with 110 chickens. Test group 1 was Example 1; test group 2 was Example 2; test group 3 was Example 3; test group 4 was control group 1; test group 5 was control group 2; test group 6 was control group 3; test group 7 was a positive control group (Tiamulin); and test group 8 was a blank group. The dosage was based on the recommended dose.

[0062] Among them, control group 1: 50 grams of Sichuan Cyathula; control group 2: 50 grams of Ephedra; control group 3: 50 grams of Stephania tetrandra, the preparation method is the same as Example 1.

[0063] 4. Test results

[0064] The results of each example are shown in the table below. The results show that the compound dosage forms with different components all have certain therapeutic effects, and Example 2 has the best therapeutic effect. The single dosage form has a poor therapeutic effect, which is not as obvious as the compound dosage form and the positive control group.

[0065] Group Number of cured cases (cases) / cure rate (%) Effective number (pieces) / Effectiveness (%) Invalid number (pieces) / Inefficiency (%) Equivalent to original drug ( / piece / g / day) Experimental Group 1 35 / 32 39 / 35 36 / 33 0.12 Experimental Group 2 70 / 64 25 / 23 15 / 13 0.12 Experimental Group 3 40 / 36 44 / 40 26 / 24 0.12 Experimental Group 4 0 / 0 17 / 15 93 / 85 0.12 Experimental Group 5 10 / 9 25 / 23 75 / 68 0.12 Experimental Group 6 0 / 0 21 / 19 89 / 81 0.12 Test Group 7 33 / 30 67 / 61 10 / 9 / Experimental Group 8 0 / 0 10 / 9 100 / 91 0

[0066] From the above test results, it can be seen that compared with the compound examples, the single examples have obvious therapeutic effects, and the therapeutic effect of the test group 3 of Example 3 is better than that of the positive control group (tylosin) of the test group 7. The compound dosage form of the present invention has an excellent therapeutic effect on Mycoplasma synoviae, among which the therapeutic effect of Example 2 is the most prominent.

[0067] Finally, it should be explained that the above embodiments are only for explanation of the present application and are not used to limit the protection scope of the present application, but are determined by the claims of the present application. For those skilled in the art, the present application can be modified and varied in various ways. Any equivalent changes or modifications made according to the essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease, characterized in that: It is prepared from the following raw materials in the following weight ratio: 40-60 parts of Cyathula, 20-40 parts of Cinnamon Bark, 50-70 parts of Stephania Tetrandrae, 40-60 parts of Ephedra, 50-70 parts of Gentiana Macrophylla, and 20-40 parts of Licorice.

2. The Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease according to claim 1, characterized in that: It is prepared from the following raw materials in the following weight ratio: 50 parts of Cyathula officinalis, 30 parts of Cortex Cinnamomi, 60 parts of Radix Tetrandrae, 50 parts of Ephedra, 60 parts of Radix Gentianae Macrophyllae, and 30 parts of Radix Glycyrrhizae.

3. The Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease according to claim 1 or 2, characterized in that: The raw drug powder, water or organic solvent extract of the raw drug are used as active ingredients, and pharmaceutically acceptable auxiliary materials or auxiliary ingredients are added to prepare a commonly used pharmaceutical preparation.

4. The Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease according to claim 3, characterized in that: The preparation is a mixture, powder, granule, tablet or pill.

5. A method for preparing the Chinese veterinary medicine composition for treating and / or preventing chicken synoviae mycoplasma disease according to any one of claims 1 to 4, characterized in that: It includes the following steps: a. Weigh the APIs of various weight ratios; b. The raw medicine is ground into powder, or boiled in water for extraction, and then pharmaceutically acceptable excipients or auxiliary ingredients are added to prepare a commonly used pharmaceutical preparation.

6. Use of the Chinese veterinary drug composition according to any one of claims 1 to 4 in the preparation of a drug for treating and / or preventing chicken synoviae mycoplasma disease.

7. The use according to claim 6, characterized in that: The medicine is a medicine for treating or / and preventing arthritis caused by wind, cold, dampness and heat obstruction.

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