A Trichoderma strain antagonistic to melon root-knot nematode disease and wilt disease and its application

Abstract

The present invention belongs to the field of microorganisms, and specifically relates to a Trichoderma strain antagonistic to melon root-knot nematode disease and wilt disease and its application. Trichoderma breve Application of HLST422‑7 in the control of root-knot nematodes and wilt diseases. Trichoderma breve There are no reports on the use of HLST422‑7 in the prevention and treatment of plant nematode diseases, and there has never been any relevant research on the combined infection of nematodes and wilt. T.short Compared with T069, Trichoderma breve HLST422‑7 has better control effects on root-knot nematodes and wilt, and can also improve plant growth in the presence of nematodes, further demonstrating the great advantages of this strain in plant disease prevention and control.

Description

Technical Field

[0001] The invention belongs to the technical field of modern agriculture, and particularly relates to a Trichoderma strain antagonistic to melon root knot nematode disease and wilt disease and application thereof. Background Art

[0002] Root-knot nematode disease and wilt are very common soil-borne diseases in melon production. Root-knot nematodes infect the roots of plants, grow and reproduce in the plant body, and form root knots of varying sizes on the main roots and fibrous roots. In severe cases, the root system swells, slowly softens and rots. Damage to the roots prevents water and nutrients from being supplied to the aboveground part of the plant, resulting in short plants, weak root systems, yellowing and wilting of the aboveground stems and leaves, and eventually death of the entire plant. Melon wilt is also a very common soil-borne disease, which is particularly serious in continuous cropping areas. It is called the "cancer" of melons and vegetables, with an incidence rate of 15-35%, and in severe cases it can reach more than 75%, or even a total crop failure. It is one of the important pathogens that cause melon yield losses. Root-knot nematodes pierce the plant's root system with their mouth needles, and enter the plant's root system with the help of secreted degrading enzymes. The wound formed after the puncture becomes a door for the wilt bacteria to enter the plant. Therefore, in fields where nematodes are seriously infested, wilt is often accompanied by the occurrence of wilt, resulting in a combined infection of root-knot nematodes and wilt, which has a huge impact on plant growth and the yield and quality of melons.

[0003] At present, biological control methods based on biocontrol bacteria can be used to control root-knot nematodes. Trichoderma is a common fungus in nature, and has good effects in the prevention and control of various diseases. There are many types of Trichoderma. Trichoderma viride, Trichoderma longibrachiatum, Trichoderma harzianum, Trichoderma spore, Trichoderma citriodora, etc. have been reported to be effective against root-knot nematodes. Trichoderma harzianum, Trichoderma spore, Trichoderma pseudokoningii, Trichoderma viride, etc. are effective against wilt. Although various biocontrol agents have certain preventive effects on the occurrence of root-knot nematodes and wilt, when root-knot nematodes and wilt occur simultaneously to produce a complex infection, there is often no single biocontrol bacterium that can successfully prevent and control them, and there is no related research on whether a single Trichoderma can effectively prevent and control them. Summary of the invention

[0004] The first aspect of the present invention aims to provide a strain of Trichoderma.

[0005] The second aspect of the present invention aims to provide a culture or metabolic product.

[0006] The third aspect of the present invention aims to provide a method for preparing the culture of the second aspect of the present invention.

[0007] The fourth aspect of the present invention aims to provide a bacterial agent.

[0008] The purpose of the fifth aspect of the present invention is to provide a method for preparing the bacterial agent of the fourth aspect of the present invention.

[0009] The sixth aspect of the present invention aims to provide the use of the Trichoderma of the first aspect, the culture of the second aspect, and the bacterial agent of the fourth aspect.

[0010] The seventh aspect of the present invention aims to provide a product.

[0011] The eighth aspect of the present invention is to provide a method. In order to achieve the above-mentioned purpose of the present invention, the technical solution adopted by the present invention is:

[0012] In a first aspect of the present invention, a strain of Trichoderma is provided, the name of which is HLST422-7, which is classified as Trichoderma short-stalked Trichoderma breve , deposited at the General Microbiology Center of China Microorganism Culture Collection Committee on September 9, 2024. The address of the depository is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), and the deposit number is: CGMCC No. 41500.

[0013] Trichoderma breve HLST422-7 colonies are actinomycetes, with dense hyphae and lighter color. Aerial hyphae are abundant and cover the entire plate, with no obvious pigment and odor. Spore clusters are yellow-green, conidia are spherical and smooth. Conidiophores are solitary, opposite or tri-whorled.

[0014] The second aspect of the present invention provides a culture or metabolite obtained by culturing the Trichoderma of the first aspect of the present invention.

[0015] The third aspect of the present invention provides a method for preparing the culture of the second aspect of the present invention, comprising inoculating the Trichoderma of the first aspect of the present invention into a culture medium and culturing the culture medium to obtain the Trichoderma.

[0016] Preferably, the culture medium comprises at least one of PDA culture medium, CMD culture medium and rice culture medium.

[0017] In some embodiments of the present invention, the culture condition is culturing at 16-37°C for 7 days. Preferably, the culture temperature is 26-30°C.

[0018] The fourth aspect of the present invention provides a bacterial agent comprising the Trichoderma of the first aspect of the present invention and / or the culture of the second aspect of the present invention.

[0019] Preferably, the bacterial agent is a solid bacterial agent or a liquid bacterial agent.

[0020] Preferably, the solid bacterial agent comprises a carrier.

[0021] Preferably, the carrier comprises at least one of a mineral material and a plant material.

[0022] Further preferably, the mineral material comprises at least one of activated carbon, clay, peat soil, vermiculite, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth.

[0023] Further preferably, the plant material comprises at least one of corn flour, soybean flour and wheat bran.

[0024] Preferably, the liquid bacterial agent includes a protective agent.

[0025] Preferably, the protective agent includes one or more of glycerol, benzoate, sorbate, polyglycol, and Tween.

[0026] Preferably, the bacterial agent is in the form of at least one of a liquid, a powder or a granule.

[0027] The fifth aspect of the present invention provides a method for preparing the microbial agent of the fourth aspect of the present invention, wherein the Trichoderma of the first aspect of the present invention and / or the culture of the second aspect of the present invention are used as active ingredients to obtain the microbial agent.

[0028] The sixth aspect of the present invention provides the use of any one of (a1) to (a3) ​​in at least one of (b1) to (b6):

[0029] (a1) the Trichoderma described in the first aspect of the present invention;

[0030] (a2) the culture according to the second aspect of the present invention;

[0031] (a3) the bacterial agent according to the fourth aspect of the present invention;

[0032] (b1) prevention and / or treatment of melon root-knot nematode disease;

[0033] (b2) Preparation of products for preventing and / or treating melon root-knot nematode disease;

[0034] (b3) prevention and / or treatment of melon wilt;

[0035] (b4) Preparation of products for preventing and / or treating melon wilt;

[0036] (b5) simultaneous prevention and / or treatment of melon root-knot nematode disease and melon wilt disease;

[0037] (b6) Preparing products for simultaneously preventing and / or treating melon root-knot nematode disease and melon wilt disease.

[0038] A seventh aspect of the present invention provides a product comprising at least one of (a1) to (a3):

[0039] (a1) the Trichoderma described in the first aspect of the present invention;

[0040] (a2) the culture according to the second aspect of the present invention;

[0041] (a3) the bacterial agent according to the fourth aspect of the present invention;

[0042] Preferably, the product may further include other active ingredients.

[0043] Preferably, the other active ingredients include but are not limited to other Trichoderma that are effective against root-knot nematode disease or wilt, such as Trichoderma viride, Trichoderma longibrachiatum, Trichoderma harzianum, Trichoderma acanthosporum, Trichoderma citriviride, Trichoderma pseudokoningii, etc.

[0044] Preferably, the other active ingredients may also include other conventional drugs effective against root-knot nematode disease or wilt disease.

[0045] Preferably, the product may further include pharmaceutically acceptable excipients.

[0046] Preferably, the pharmaceutically acceptable excipients include at least one of a solvent, a colorant, a stabilizer, a preservative, a pH adjuster, a buffer, a surfactant, a foaming agent, a defoaming agent, a thickener, and a carrier.

[0047] The eighth aspect of the present invention provides any one of methods (c1) to (c3), comprising the step of using the product described in the seventh aspect of the present invention;

[0048] (c1) Methods for preventing and / or treating melon root-knot nematode disease;

[0049] (c2) methods for preventing and / or treating melon wilt;

[0050] (c3) Methods for the simultaneous prevention and / or treatment of melon root-knot nematode disease and melon wilt disease.

[0051] The beneficial effects of the present invention are:

[0052] The present invention aims to solve the problem of combined infection of melon root knot nematodes and wilt disease, and proposes a short-stalked Trichoderma Trichoderma breve Application of HLST422-7 in the control of root-knot nematodes and wilt diseases.

[0053] Trichoderma breve There are no reports on the use of HLST422-7 in the prevention and treatment of plant nematode diseases, and there has never been any relevant research on the combined infection of nematodes and wilt. T. longibrachiatum , different subspecies of the same species T. breve Compared with T069, Trichoderma breveHLST422-7 has better control effects on root-knot nematodes and wilt, and can also improve plant growth in the presence of nematodes, further demonstrating the great advantages of this strain in plant disease prevention and control. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0055] Figure 1 for Trichoderma breve The morphological identification results of HLST422-7, where A is the colony morphology on PDA medium; B is the spore cluster morphology on PDA medium; C is the spore morphology; D is the conidiophore morphology.

[0056] Figure 2 for T. breve Antagonistic effect of HLST422-7 on melon wilt pathogen on plates, where A is the control result and B is the inoculated result T. breve Results after HLST422-7.

[0057] Figure 3 for T. breve Effects of HLST422-7 and clean water treatment on the growth and root knotting of melon, where A is T. short A is the growth of plants in the HLST422-7 treatment group; B is the growth of plants in the water control group; C is T. breve The root system of the HLST422-7 treatment group has root knots; D is the root system of the clear water control group has root knots. DETAILED DESCRIPTION

[0058] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0059] The strains and biological materials used in the present invention are from the following sources:

[0060] Trichoderma HLST422-7, classified as Trichoderma short-stalked Trichoderma breve :The applicant deposited the product with the General Microbiology Center of China Microorganism Culture Collection on September 9, 2024. The address of the depository is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), and the deposit number is: CGMCC No. 41500.

[0061] Southern root-knot nematode and rice root-knot nematode of the Poaceae family: All nematodes were obtained through propagation and cultivation in this laboratory. The southern root-knot nematode was propagated through tomato, and the root-knot nematode of the Poaceae family was propagated through rice. Both are conventional materials in this field.

[0062] Fusarium oxysporum melon-specific type ( Fusarium oxysporum f. sp. melon ): Conventional materials in this field can be obtained by conventional means.

[0063] Trichoderma longibrachiatum: Conventional materials in this field can be obtained by conventional means.

[0064] Trichoderma sp. T069, classified as Trichoderma brevis Trichoderma breve: It was deposited by the inventor at the General Microbiology Center of China Microorganism Culture Collection Committee on September 28, 2023. The address of the depository is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), and the deposit number is: CGMCC No.40838.

[0065] Example 1 Trichoderma breve Isolation, culture and identification of HLST422-7

[0066] T. breve HLST422-7 was collected from a Hami melon shed in Jinwei Village, Xincun Town, Lingshui Li Autonomous County, Hainan Province. Soil was collected from the rhizosphere of plants with Hami melon root-knot nematodes. The surface soil of the rhizosphere was removed, and five sampling points were collected from each shed. 100 g of the rhizosphere soil below 3 cm was collected and placed in a ziplock bag and brought back to the laboratory for storage at 4°C.

[0067] The dilution plate method was used to isolate Trichoderma. 10 g of soil sample was placed in a 50 mL triangular flask, 90 mL of sterile water was added, and the mixture was shaken at 200 r / min for 30 min. The supernatant was diluted 10 times and 100 times, that is, 1 mL of supernatant was added to 9 mL of sterile water and mixed (diluted 10 times), and then 1 mL was added to 9 mL of sterile water (diluted 100 times). 200 μL of the 10-fold and 100-fold dilutions were evenly spread on 9 cm Bengal red culture medium plates, and placed in a constant temperature incubator at 28°C for culture. After 24-48 hours, the mycelium with Trichoderma morphology was picked and continued to be cultured on PDA plates.

[0068] The primers for molecular biology sequencing of Trichoderma are: fRPB2-5f: 5'-GAYGAYMGWGATCAYTTYGG-3' (SEQ ID NO: 1); fRPB2-7cr: CCCATRGCTTGYTTRCCCAT (SEQ ID NO: 2). After sequencing and comparing with the NCBI database, it was found that Trichoderma breve

[0069] The morphological results of the strain are as follows Figure 1 As shown. Figure 1 It can be seen T. breve HLST422-7 The colony is actiform, with dense hyphae and light color. Aerial hyphae are abundant and cover the entire plate, with no obvious pigment and odor. The spore clusters are yellow-green, and the conidia are spherical and smooth. The conidiophores are solitary, opposite or in three whorls.

[0070] right Trichoderma breve HLST422-7 has undergone the preservation procedure and was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on September 9, 2024, with the preservation number: CGMCC No. 41500. The name is HLST422-7, and the classification name is short-stalked Trichoderma Trichoderma breve.

[0071] Example 2 Determination of nematicidal activity of fermentation liquid against second-instar larvae of root-knot nematodes

[0072] 1. Liquid fermentation culture

[0073] The colony cultured on PDA in Example 1 was punched with a 5 mm puncher to obtain three bacterial cakes at the edge of the colony and placed in 150 mL PD liquid culture medium, shaken and cultured at 28°C and 180 rpm for 7 days to obtain a fermentation broth. The fermentation product was centrifuged at 10,000 rpm for 10 min, the supernatant was taken, and the bacteria were removed by a 0.22 μm sterile filter membrane, and the filtrate was collected to obtain the treated fermentation broth for later use.

[0074] 2. Test of southern root-knot nematode

[0075] Meloidogyne incognita ( Meloidogyne incognita ), Rice root-knot nematode ( Meloidogyne grass-like plant ) were obtained by culture and propagation in our laboratory. Mature and plump oocysts were selected under a stereomicroscope, disinfected with 0.5% sodium hypochlorite for 5 min and then rinsed with sterile water for 5 times. After disinfection, the oocysts were placed in an incubation pool and cultured in a constant temperature box at 25°C in the dark. Freshly hatched nematodes were collected daily for nematicidal activity testing.

[0076] 3. Nematicidal activity test

[0077] 1 mL of fermentation liquid was added to a 24-well plate, and 20 μL (about 30-40) second-instar larvae (J2) suspension of root-knot nematodes was added to each well. Clean water was used as the control, and each treatment was repeated three times. The death of nematodes was observed at 24 h, 48 h, and 72 h. The nematodes stopped moving, the body was stiff, and those that did not move after being stimulated with a hair needle were considered dead, and the mortality rate was calculated. The test results are shown in Table 1.

[0078] Table 1 T. breve Contact killing ability of HLST422-7 against second-instar larvae of root-knot nematodes

[0079]

[0080] Note: * indicates that the control group and the treatment group P The difference was significant at the <0.01 level ( t -test).

[0081] From the results in Table 1, we can see T. breve The fermentation liquid of HLST422-7 had a direct contact killing effect on southern root-knot nematode J2, with mortality rates of 31.11%, 45.66% and 72.63% at 24 h, 48 h and 72 h, respectively. T. breve The mortality rates of rice root-knot nematodes treated with HLST422-7 for 48 h and 72 h were 94.71% and 97.51%, respectively.

[0082] Example 3 Determination of the inhibitory activity of the fermentation broth against Fusarium oxysporum melon-specific type

[0083] Will T. breve HLST422-7 and Fusarium oxysporum melon-specific type ( Fusarium oxysporum f.sp. melon ) were inoculated on PDA medium and cultured at 28℃ for 72 h. A 5 mm punch was used to punch bacterial cakes from the edge of the colony and inoculated on both sides of the PDA plate at the same time. The distance between the two bacterial cakes was 8 cm. The plate was placed in a 28℃ incubator for culture. After about 7 days, the control group inoculated with only Fusarium oxysporum but not Trichoderma was full of the plate, and the inhibition rate was calculated. Three replicates were set.

[0084] Inhibition rate (%) = [(pathogen radius of control group - pathogen radius of treatment group) / (pathogen radius of control group - bacterial cake radius)] × 100%.

[0085] The results are as follows Figure 3 As shown, the present invention T. breve HLST422-7 has good antagonism to the melon-specific type of Fusarium oxysporum, with an inhibition rate of 83.22%.

[0086] Example 4 T. breve Determination of the efficacy of HLST422-7 against melon root-knot nematode disease

[0087] Taking the control of melon root-knot nematode disease in potted plants as an example, the experiment used a concentration of 1×10 7 CFU / mL T. shortHLST422-7 spore suspension, the Trichoderma strain was inoculated on a PDA plate and cultured at 28°C for 96 h. The spores on the plate were scraped with a spreader to prepare a concentration of 1×10 7 CFU / mL of spore suspension. The soil used was a mixture of nutrient matrix and sand in a ratio of 1:1. It was sterilized in a dry heat sterilizer at 165°C for 2 h and then placed in a plastic pot. The melon seedling variety was Emerald. After raising the seedlings in advance, they were transplanted into culture pots after the seedlings grew two leaves. One week after planting, 10 mL of spore suspension was inoculated in each pot. One week later, 200 root-knot nematodes were inoculated. Seven days later, the spore suspension was inoculated again. 21 days after the nematode inoculation, the number of root knots on the roots was investigated, and the plant height, root length, fresh weight, root fresh weight and other indicators were measured. The number of root knots per gram of root fresh weight was calculated. Inoculate with the same concentration of Trichoderma longibrachiatum and different subspecies of the same species T. breve T069 spore suspension was used as the control group.

[0088] Table 2 T. breve Effect of HLST422-7 on root-knot nematode control in potted plants

[0089]

[0090] Note: Duncan method was used for multiple comparisons. Different letters represent differences between control and treatment groups. P The difference was significant at the <0.05 level.

[0091] It can be seen from Table 2 that the present invention T. breve HLST422-7 can significantly reduce the incidence of root-knot nematodes and the number of root knots. T. breve The number of root knots after HLST422-7 treatment was 53, significantly lower than the 99 in the clear water control group, and T. longibrachiatum and T. breve T069 control. T. breve The number of root nodes per gram of root fresh weight in the HLST422-7 treatment group was 52, which was significantly lower than 488 in the clear water control group, and the inhibition rate was 89.3%. T. longibrachiatum and T. breve The number of root knots per gram of root fresh weight after T069 treatment was reduced by 54.9% and 61.6% respectively compared with the water treatment group. T. short T069 and T. breve HLST422-7 are both short-stalked Trichoderma, but there are still significant differences in the biocontrol efficacy of different strains. T. breve HLST422-7 has a good control effect on melon root-knot nematodes.

[0092] Table 3 T. breve Effects of HLST422-7 on the growth of plants infected by root-knot nematodes

[0093]

[0094] Note: Duncan method was used for multiple comparisons. Different letters represent differences between control and treatment groups. P The difference was significant at the <0.05 level.

[0095] From Table 3, we can see that T. breve The plant height, fresh weight and root fresh weight of the bacteria treated with HLST422-7 after inoculation with nematodes were significantly increased compared with the water control. T. breve T069 control group; root length, fresh weight, root fresh weight were higher than T. longibrachiatum Control group. T. breve Treatment of melon plants with HLST422-7 can promote the growth of plant height, plant fresh weight and root fresh weight.

[0096] Example 5 T. breve Determination of the protective effect of HLST422-7 on melon wilt

[0097] Taking the prevention and control of melon wilt in potted plants as an example, the soil treatment method and fermentation liquid used were the same as those in Example 4. After the melon seedlings were raised in advance, they were transplanted into the culture pots after they grew two leaves. One week after planting, each pot was inoculated with 10 mL (1×10 7 CFU / mL), and one week later, 5 mL of Fusarium oxysporum spore suspension (spore concentration was 1×10 5 CFU / mL), with clean water as the control group, and each treatment was repeated 5 times. The incidence of Fusarium wilt was detected 21 days after inoculation, and the leaf surface disease was graded. The grading standard is:

[0098] Grade 0: no symptoms;

[0099] Level 1: Less than 25% of the leaf area is slightly wilted;

[0100] Level 2: 25% to 50% of the leaf area wilts and loses water;

[0101] Level 3: More than 50% of the leaf area wilts and loses water;

[0102] Level 4: Plant death.

[0103] .

[0104] Table 4 T. breve Effect of HLST422-7 on the prevention of melon wilt in potted plants

[0105]

[0106] Note: Duncan method was used for multiple comparisons. Different letters represent differences between control and treatment groups. PThe difference was significant at the <0.05 level.

[0107] From Table 4, it can be seen that the present invention has good potted plant performance. T. breve The leaf disease grade of the HLST422-7 strain treatment group was 1.17, and the disease index was 29.2, while the leaf disease grade of the control group was 2.50, and the disease index was 62.5. T. long-armed After treatment in the control group, the leaf disease grade was 1.83 and the disease index was 45.8. T. breve The leaf disease incidence of plants treated with HLST422-7 was significantly lower than that of the water control and T. longibrachiatum Therefore, T. short Treatment with HLST422-7 strain can significantly alleviate the incidence of melon wilt.

[0108] Example 6 T. breve Determination of the efficacy of HLST422-7 against combined infection of melon wilt and root-knot nematode

[0109] Taking the melon potted plant experiment as an example, the soil treatment method and fermentation liquid used were the same as those in Example 4. After the melon seedlings were raised in advance, they were transplanted into the culture pots after they grew two leaves. One week after planting, each pot was inoculated with 10 mL of Trichoderma spore suspension, and one week later, 5 mL of Fusarium oxysporum spore suspension was inoculated (spore concentration was 1×10 5 CFU / mL), one week later, 200 southern root-knot nematodes were inoculated, and then Trichoderma spore suspension was inoculated every 7 days. 21 days after nematode inoculation, the number of root knots in the plant roots and the incidence of leaf wilt were detected, and the leaf disease was graded.

[0110] Table 5 T. breve Effect of HLST422-7 on combined infection of melon wilt and root-knot nematode in potted plants

[0111]

[0112] Note: Duncan method was used for multiple comparisons. Different letters represent differences between control and treatment groups. P The difference was significant at the <0.05 level.

[0113] As shown in Table 5, in the potted plant test of the present invention inoculated with root-knot nematodes and wilt pathogens, the number of root knots in the control group was 74.8, and the number of root knots per gram of root fresh weight was 53.7; T. breve The number of root knots in the HLST422-7 treatment group was 31.0, and the number of root knots per gram of root fresh weight was 19.3, which were significantly lower than those in the water control group ( P <0.05), the number of root knots per gram of root fresh weight was significantly lower than T. long-armedControl group. The leaf surface of the water control group with wilt disease was graded as level 3, with a disease index of 75; T. long-armed The leaf surface grade of Fusarium wilt in the control group was 2, with a disease index of 50; T. breve The leaf surface grade of Fusarium wilt in the HLST422-7 treatment group was 1.2, which was significantly lower than that in the water control group and T. longibrachiatum In the control group, the disease index was 30. The results showed T. breve HLST422-7 can effectively control the combined incidence of root-knot nematodes and wilt, and its effect is better than that of Trichoderma longifolia T. longibrachiatum .

[0114] Table 6 T. breve Effects of HLST422-7 on the growth of melon under infection of root-knot nematodes and Fusarium wilt

[0115]

[0116] Note: Duncan method was used for multiple comparisons. Different letters represent differences between control and treatment groups. P The difference was significant at the <0.05 level.

[0117] As shown in Table 6, the present invention has a good effect in the potted plant test of composite inoculation of root-knot nematodes and wilt pathogens. T. short The fresh weight and root dry weight of HLST422-7 treated plants were significantly higher than those of the water control group and T. longibrachiatum The fresh weight of roots in the control group was significantly higher than that in T. longibrachiatum There was no significant difference between the control and the water control.

Claims

1. A strain of Trichoderma, named HLST422-7, classified as Trichoderma short-stalked Trichoderma breve , deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on September 9, 2024, with the deposit number: CGMCC No.41500.

2. A culture comprising the Trichoderma according to claim 1.

3. The method for preparing the culture according to claim 2, comprising the following steps: Inoculate the Trichoderma according to claim 1 into a culture medium and culture to obtain; The culture conditions are 16-37°C. A bacterial agent comprising the Trichoderma according to claim 1.

5. The bacterial agent according to claim 4, characterized in that: The bacterial agent is a solid bacterial agent or a liquid bacterial agent.

6. A method for preparing the bacterial agent according to claim 4 or 5, wherein the Trichoderma according to claim 1 is used as an active ingredient to obtain the bacterial agent.

7. The application of any one of (a1) to (a3) ​​in at least one of (b1) to (b6); (a1) the Trichoderma according to claim 1; (a2) the culture according to claim 2; (a3) the bacterial agent according to claim 4 or 5; (b1) prevention and / or treatment of melon root-knot nematode disease; (b2) Preparation of products for preventing and / or treating melon root-knot nematode disease; (b3) prevention and / or treatment of melon wilt; (b4) Preparation of products for preventing and / or treating melon wilt; (b5) simultaneous prevention and / or treatment of melon root-knot nematode disease and melon wilt disease; (b6) Preparation of products for the simultaneous prevention and / or treatment of melon root-knot nematode disease and melon wilt disease; The melon root-knot nematode disease is a melon root-knot nematode disease caused by the southern root-knot nematode.

8. A product comprising the bacterial agent according to claim 4 or 5.

9. Any method of (c1) to (c3), comprising the step of using the product of claim 8; (c1) Methods for preventing and / or treating melon root-knot nematode disease; (c2) methods for preventing and / or treating melon wilt; (c3) Methods for the simultaneous prevention and / or treatment of melon root-knot nematode disease and melon wilt disease; The melon root-knot nematode disease is a melon root-knot nematode disease caused by the southern root-knot nematode.

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