Preparation method of factory-like tremella aurantialba liquid strain

Abstract

The invention relates to a preparation method of a factory-like tremella aurantialba liquid strain, which comprises the following steps: inoculating a tremella aurantialba mushroom stock seed into a first-stage culture medium for regulation culture to obtain a primary liquid strain, and then carrying out propagation culture on the liquid strain in a second-stage culture medium, the first-stage culture medium is specifically prepared by adding 30-35g of wheat, 10g of corn flour, 10-15g of glucose, 5g of maltose, 20-25mg of chitosan and 1.5-2g of magnesium sulfate into 1L of water, preparing a first-stage culture medium from 3-4g of potassium dihydrogen phosphate; according to the method, specific mushrooms are selected, and a specific culture medium is adopted for conditioning culture, so that the proportion coordination of associated fungi and tremella auramtialba is adjusted, the associated fungi and the tremella auramtialba are stable and unchanged in the subsequent propagation culture process, the problem of strain failure in the culture process is effectively solved, and the growth consistency and excellent quality of the liquid strain after inoculation are ensured; a single strain reaches 195 g, the biological efficiency reaches up to 90% or above, and industrialized production is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus cultivation, and in particular to a method for preparing industrialized golden fungus liquid strains. Background Art

[0002] Golden ear, also known as golden ear fungus, is the fruiting body of the Tremella fuciformis plant. It is shaped like a human brain and is golden yellow. The shape of golden ear is similar to that of wood ear, hence the name "golden ear". Golden ear is a rare edible fungus. It is very popular because of its bright color, fragrant smell, soft and delicate taste, and the market demand is gradually increasing. Golden ear is rich in carbohydrates, protein, crude fiber, and 18 kinds of amino acids and other nutrients. It has all the essential amino acids for the human body. Its nutritional value is the highest among the three ears, thanks to Tremella fuciformis and black wood ear. In addition, golden ear also has pharmacological effects such as expectorant, cough relief, anti-inflammatory, asthma sedation, lowering blood sugar, and improving human hematopoietic function and immunity.

[0003] Golden ear is a weak wood-decaying edible fungus. The ability of a single pure mycelium to decompose crude fiber is very weak, so it needs associated bacteria to decompose and transform the difficult-to-decompose nutrients for the golden ear to absorb and utilize. Therefore, the golden ear fungus actually contains two types of fungi, golden ear and associated bacteria. Only the golden ear fungus containing associated bacteria can grow fruiting bodies. Common golden ear associated bacteria include blood-stain tough leather fungus ( Stereum sanguinolentum )、Hairy leather fungus( S.hirsutum ) and Lederma tenuissima ( S. pubescens ).

[0004] In the prior art, most of the original hairy leather fungus liquid strain + golden ear fruiting body mode is used for production, but the use of solid strain production faces the problems of slow strain germination, long growth cycle, high contamination rate, and inconsistent strain age. In recent years, yeast-like spores + leather fungus liquid strains are used to mix into mixed liquid strains for production, but the matching ratio and mixing time are difficult to control, which easily leads to a decrease in the effectiveness of the liquid strains. The phenomenon of no mushrooms often occurs during the production process, which is not suitable for factory production. In the prior art, there is also a method of using a mother strain to prepare a pure liquid strain. For example, in CN108990704 A, golden ear is inoculated into a liquid culture medium to prepare a liquid stock, and then the liquid stock is inoculated into a fermentation tank for fermentation and culture to prepare golden ear liquid strain. However, the overall culture cycle of this method is long and the culture steps are complicated. However, it is clear in the art that the ratio of golden ear to hairy leather fungus in the golden ear strain, and the changes in the growth rates of the two strains during the preparation of the liquid strain have a great impact on the effectiveness of the prepared strain and the subsequent growth of golden ear. The long and complicated culture process is likely to lead to an imbalance in the ratio of golden ear to hairy leather fungus in the strain, and the growth of the two bacteria is unstable. In the expansion culture, the ratio of golden ear to hairy leather fungus is poorly stable, which will make it difficult to ensure the growth consistency of the strain during large-scale production. Summary of the invention

[0005] Based on the above technical problems, the present invention aims to provide a method for preparing a factory-based golden ear liquid strain. The method effectively stabilizes the ratio of golden ear and hairy leather fungus in the liquid strain, and the golden ear and hairy leather fungus have excellent stability during the propagation process. The composite mycelium of the liquid strain germinates quickly and stably, the mycelium amount of the two fungi is controllable, and the strain growth consistency is excellent.

[0006] The purpose of the present invention is achieved through the following technical solutions: A method for preparing a factory-scale golden ear liquid strain is characterized in that: golden ear mushroom original species are inoculated into a primary culture medium for conditioning culture to obtain primary liquid strains, and then the liquid strain secondary culture medium is expanded and cultured, wherein the primary culture medium is specifically prepared by adding 30-35g of wheat, 10g of corn flour, 10-15g of glucose, 5g of maltose, 20-25mg of chitosan, 1.5-2g of magnesium sulfate, and 3-4g of potassium dihydrogen phosphate into 1L of water to prepare the primary culture medium.

[0007] The wheat is boiled in water for 20 minutes and then mixed with other ingredients.

[0008] Furthermore, the golden ear mushroom seed is a golden ear fruiting body with a pink color and a cultivation period of 65 to 80 days. Specifically, a thin layer on the surface of the mushroom is selected as the original seed, and 4 pieces (1*1 cm in size) are inoculated in 1L of primary culture medium.

[0009] Furthermore, preferably, the cultivation period of the above-mentioned golden ear mushroom is 70 to 75 days.

[0010] Furthermore, the culture temperature is adjusted to 19-21° C., the culture speed is 150-250 rpm, and the culture period is 3-4 days.

[0011] Furthermore, the secondary culture medium used in the propagation culture is 1L of water with 10g of culture material powder, 20g of glucose, 1g of fish peptone, 1.5g of magnesium sulfate, and 3g of potassium dihydrogen phosphate, wherein the culture material powder is the fine powder obtained by drying and grinding the culture material residue after the golden ear cultivation and fruiting (the culture material is composed of 78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, and 1% light calcium carbonate).

[0012] Furthermore, the multiplication culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days.

[0013] The ratio of golden ear fungus and associated bacteria in the strain has a significant impact on the quality of the strain. If the proportion of associated bacteria is too high, the strain will face slow germination, long growth cycle, and poor consistency of fruiting body growth. If the proportion of golden ear fungus is too high, it cannot decompose and utilize the nutrients in the cultivation matrix well, resulting in the problem of no mushroom production. In the long-term cultivation process, we found that when the ratio of associated bacteria and golden ear fungus in the inoculated strain is coordinated, the germination effect of the strain and the growth quality of golden ear fruiting bodies are relatively excellent. In the process of preparing liquid strains using original strains, it was found that after the original strain was cultured and expanded, the ratio of associated bacteria and golden ear fungus in the strain was basically unbalanced, which would lead to unsatisfactory subsequent cultivation effects, small size of golden ear fruiting bodies, poor meat elasticity, dull color and other problems.

[0014] In the present invention, golden ear fruiting bodies with a cultivation period of 65 to 80 days are used as seed mushrooms, and a small amount of maltose and a trace amount of chitosan are added when preparing the primary culture medium. Since different sugar sources have different growth regulating effects on different microorganisms, the proportion coordination of the associated bacteria and golden ear fungus in the bacterial liquid after the primary culture is effectively adjusted, and the participation of a trace amount of chitosan in the culture improves the proportion stability of the associated bacteria and the golden ear fungus, so that the proportion relationship is stable in the subsequent expansion and propagation culture without obvious changes. This makes the bacterial liquid obtained by the expansion and propagation culture have excellent consistency with the bacterial liquid of the primary culture, and the quality of the golden ear cultivated by inoculation is guaranteed.

[0015] Most specifically, a method for preparing a liquid fungus strain of Tremella fuciformis in a factory is characterized by comprising the following steps: (1) Preparing stock Take golden ear fruiting bodies with pink color and cultivation period of 65-80 days as seed mushrooms, and pick a thin layer on the surface of the seed mushrooms as the original seed; (2) Conditioning and culture The original seed is inoculated into a primary culture medium for conditioning culture, wherein the primary culture medium is prepared by adding 30-35 g of wheat, 10 g of corn flour, 10-15 g of glucose, 5 g of maltose, 20-25 mg of chitosan, 1.5-2 g of magnesium sulfate, and 3-4 g of potassium dihydrogen phosphate into 1 L of water, and 4 pieces (1*1 cm in size) of the original seed are inoculated into 1 L of the primary culture medium, the culture medium is sterilized at 123° C. before inoculation, the culture temperature is adjusted to 19-21° C., the culture speed is 150-250 rpm, and the culture period is 3-4 days; (3) Propagation and cultivation The primary bacterial strain liquid obtained by conditioning culture in step (2) is inoculated into a secondary culture medium for propagation culture. The secondary culture medium is prepared by adding 10 g of fine powder of cultivation material, 20 g of glucose, 1 g of fish peptone, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of the secondary culture medium. The culture medium is sterilized at 123° C. before inoculation. The fine powder of cultivation material is obtained by drying and grinding the residue of cultivation material after the fruiting of Tremella fuciformis culture. The propagation culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days. The cultivation material consists of 78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, and 1% light calcium carbonate.

[0016] The present invention has the following technical effects: The present invention selects specific mushroom species and adopts specific culture medium for conditioning culture, thereby adjusting the proportion coordination of the associated bacteria and the golden ear mushroom, and making it stable in the subsequent expansion and culture process, effectively solving the problem of strain failure in the culture process, and ensuring the growth consistency of the liquid strain after inoculation, with excellent quality, a single strain reaches 195g, and the biochemical efficiency is as high as more than 90%, realizing factory production. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 : Physical picture of the liquid strain prepared by the present invention.

[0018] Figure 2 : A cross-sectional view of a Tremella fuciformis fruiting body cultivated by inoculation with liquid spawn prepared by the present invention.

[0019] Figure 3 : A comparison chart of golden ear fruiting bodies cultivated with the liquid strain prepared by the present invention and the liquid strain in the prior art. DETAILED DESCRIPTION

[0020] The present invention is described in detail below through examples. It is necessary to point out that the following examples are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Those skilled in the art can make some non-essential improvements and adjustments to the present invention based on the above-mentioned contents of the present invention.

[0021] Example 1 A method for preparing a liquid fungus strain of Tremella fuciformis in a factory, comprising the following steps: (1) Preparing stock Take golden ear fruiting bodies with pink color and cultivation period of 65-80 days as seed mushrooms, and pick a thin layer on the surface of the seed mushrooms as the original seed; (2) Conditioning and culture The original seed is inoculated into a primary culture medium for conditioning culture. The primary culture medium is prepared by adding 30 g of wheat, 10 g of corn flour, 12 g of glucose, 5 g of maltose, 22 mg of chitosan, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of water. Four pieces of original seed (1*1 cm in size) are inoculated into 1 L of the primary culture medium. The culture medium is sterilized at 123° C. before inoculation. After inoculation, the culture temperature is adjusted to 20° C., the culture speed is 200 rpm, and the culture cycle is 3 days. At this time, the color changes to brown-red, with a light wine aroma, and the mycelium fragments are small and evenly distributed in the culture medium; (3) Propagation and cultivation The primary bacterial strain liquid obtained by conditioning and culturing in step (2) is inoculated into a secondary culture medium for propagation culture. The secondary culture medium is prepared by adding 10 g of fine powder of cultivation material, 20 g of glucose, 1 g of fish peptone, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of the secondary culture medium. The culture medium is sterilized at 123° C. before inoculation. The fine powder of cultivation material is obtained by drying and grinding the residue of cultivation material after the fruiting of Tremella fuciformis cultivation. The propagation culture is carried out at a rotation speed of 600 pm and 19° C. for 3 days to obtain liquid bacterial strains. The cultivation material is composed of 78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, and 1% light calcium carbonate.

[0022] The liquid strain prepared in this embodiment is as follows Figure 1 As shown, it can be seen that the bacterial liquid is uniformly brown-red in color, has a light wine aroma, and the mycelium fragments are small and evenly distributed in the culture medium without floating or sinking. Figure 2 As shown, its interior is solid, crystal orange-yellow in color, with evenly distributed white lines and delicate texture.

[0023] In order to verify the influence of the selection of seed mushrooms on the quality of the subsequently prepared liquid spawn, we used golden ear fruiting bodies with different cultivation cycles as seed mushrooms, specifically, the cultivation cycles were 40-55 days (excluding 55), 55-65 days (excluding 65), 65-80 days (excluding 80) and more than 80 days. According to the method of Example 1, the bacterial liquid was obtained through primary culture (conditioning culture) and secondary culture (propagation culture), and the bacterial liquid was tested. Then, the bacterial liquid after the secondary culture (propagation culture) was inoculated with the same cultivation material and cultivated under the same environment.

[0024] The cultivation materials used are: (78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, 1% light calcium carbonate, dry weight of each bag 650g, inoculation volume 1.5mL) The mycelial growth, ear emergence, fruiting body quality, biological efficiency and other properties of Tremella fuciformis were observed and statistically analyzed. The results are shown in Table 1.

[0025] Table 1:

[0026] In the present invention, by selecting mushrooms of a specific period and conditioning them through primary culture medium, the ratio of hairy leather fungus and golden ear fungus in the strain and the properties of the bacterial liquid can be adjusted to a state suitable for the growth of golden ear fungus, so that the properties and ratio of hairy leather fungus and golden ear fungus are stable in the subsequent expansion process. Compared with mushrooms of 65 to 80 days, the ratio of hairy leather fungus and golden ear fungus is seriously unbalanced in the bacterial liquid obtained by the expansion culture of mushrooms of other cultivation periods after primary culture conditioning, and some bacterial liquids have poor uniformity of properties, resulting in unsatisfactory subsequent inoculation and cultivation effects. The golden ear cultivation period of the liquid strain culture prepared by the mushrooms of 65 to 80 days is shorter, the fruiting body quality is excellent, and the biological efficiency is also significantly better than other groups.

[0027] Comparative Example 1 Compared with Example 1, the maltose in the primary culture medium was replaced by an equal amount of sucrose, and the rest of the formula and culture steps were the same as those in Example 1.

[0028] Comparative Example 2 Compared with Example 1, glucose was not added to the primary culture medium, but chitosan was used instead. The rest of the formula and steps were the same as those in Example 1.

[0029] The liquid strains obtained in Example 1, Comparative Example 1 and Comparative Example 2 were inoculated into the same cultivation material, and cultivation management was performed under the same environment. A blank group was set as a control. Compared with Example 1, the maltose and chitosan in the primary culture medium of the blank group were all replaced with glucose. The remaining formulas and steps were consistent with those in Example 1. The cultivation was performed according to the same cultivation material and cultivation management as above. The results are shown in Table 2.

[0030] Table 2:

[0031] It can be seen that the difference in quality between the bacterial liquid obtained by conditioning culture of the primary culture medium and the liquid strain obtained by further multiplication culture is very small, the stability is excellent, the golden ear obtained by inoculation cultivation is excellent, and the liquid strain after multiplication culture well maintains the strain properties of the primary culture, realizing the factory production of golden ear, and the golden ear obtained by cultivation is excellent. In the bacterial liquid obtained by the primary culture process in Comparative Example 1, the ratio of hairy leather fungus and golden ear fungus is unbalanced, and the properties of the bacterial liquid are not ideal. After further multiplication culture, it is found that the ratio stability of hairy leather fungus and golden ear fungus is extremely poor, and the ratio difference is further increased, resulting in a large range of mushrooms. In Comparative Example 2, although the ratio of hairy leather fungus and golden ear is adjusted within a relatively ideal range during primary culture, the bacterial liquid has poor properties. In the further multiplication process, the ratio of hairy leather fungus and golden ear is seriously unbalanced, and the liquid strain obtained cannot well guarantee the strain properties of the primary culture medium, resulting in unsatisfactory subsequent mushroom production.

[0032] Since the prior art in CN 117716934 A is similar to the present invention, and also uses seed mushrooms to prepare liquid strains through two-step inoculation and cultivation, we also use the same seed mushrooms as in Example 1, cut the original seeds, and carry out strain liquid culture and fermentation tank culture according to the culture method of Example 1 in CN117716934 A to obtain liquid strains. The liquid strains were inoculated into the same cultivation materials as in Example 1 and managed under the same environment. The golden ear fruiting bodies obtained by inoculating and cultivating the strains and the golden ear fruiting bodies obtained by inoculating and cultivating the liquid strains prepared in Example 1 of the present invention are shown in the figure below. Figure 3 As shown, the upper part is the golden ear fruiting body obtained by cultivating the liquid spawn in Example 1 of the present invention, and the cultivation period (mycelium growth + fruiting body formation) is 50 days, and the lower part is the golden ear fruiting body obtained by cultivating the liquid spawn in CN 117716934 A, and the cultivation period is 62 days. It can be clearly seen from the figure that the golden ear fruiting body obtained by cultivating the liquid spawn of the present invention is orange-yellow in color, large in size, and uniform, while the liquid spawn of CN 117716934 A is smaller in overall size, and the size uniformity is also poor, the size difference is more obvious, the color is white and light, and it is more compact and less elastic to the naked eye. According to statistics, its bioconversion efficiency is 50.19%, while the bioconversion efficiency in Example 1 of the present invention is 90.46%.

[0033] Example 2 A method for preparing a liquid fungus strain of Tremella fuciformis in a factory, comprising the following steps: (1) Preparing stock Take golden ear fruiting bodies with pink color and 65-day cultivation period as seed mushrooms, and pick a thin layer on the surface of the seed mushrooms as the original seed; (2) Conditioning and culture The original seed is inoculated into a primary culture medium for conditioning culture, wherein the primary culture medium is prepared by adding 35 g of wheat, 10 g of corn flour, 15 g of glucose, 5 g of maltose, 20 mg of chitosan, 2 g of magnesium sulfate, and 4 g of potassium dihydrogen phosphate into 1 L of water, and 4 pieces of original seed (1*1 cm in size) are inoculated into 1 L of the primary culture medium, the culture medium is sterilized at 123° C. before inoculation, and the culture temperature is adjusted to 19-21° C. after inoculation, the culture speed is 150 rpm, and the culture cycle is 4 days; (3) Propagation and cultivation The primary bacterial strain liquid obtained by conditioning and culturing in step (2) is inoculated into a secondary culture medium for propagation culture. The secondary culture medium is prepared by adding 10 g of fine powder of cultivation material, 20 g of glucose, 1 g of fish peptone, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of the secondary culture medium. The culture medium is sterilized at 123° C. before inoculation. The fine powder of cultivation material is obtained by drying and grinding the residue of cultivation material after the fruiting of Tremella fuciformis cultivation. The propagation culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days to obtain liquid bacterial strains. The cultivation material is composed of 78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, and 1% light calcium carbonate.

[0034] The liquid spawn prepared in this example has a uniform brown-red color, a light wine aroma, small mycelium fragments, and is evenly distributed in the culture medium without floating or sinking. The golden ear fruiting bodies obtained by inoculating and cultivating the liquid spawn are brain-shaped, bright in color, orange-yellow or orange-red, and elastic to the touch. Each fruiting body weighs 196 g and has an average diameter of 11.2 cm.

[0035] Example 3 A method for preparing a liquid fungus strain of Tremella fuciformis in a factory, comprising the following steps: (1) Preparing stock Take golden ear fruiting bodies with pink color and a cultivation period of 78 days as seed mushrooms, and pick a thin layer on the surface of the seed mushrooms as the original seed; (2) Conditioning and culture The original seed is inoculated into a primary culture medium for conditioning culture, wherein the primary culture medium is prepared by adding 32g of wheat, 10g of corn flour, 10g of glucose, 5g of maltose, 25mg of chitosan, 1.5g of magnesium sulfate, and 3g of potassium dihydrogen phosphate into 1L of water, and 4 pieces (1*1cm in size) of the original seed are inoculated into 1L of the primary culture medium, and the culture medium is sterilized at 123°C before inoculation, and the culture temperature is adjusted to 19-21°C after inoculation, the culture speed is 250rpm, and the culture cycle is 4 days; (3) Propagation and cultivation The primary bacterial strain liquid obtained by conditioning and culturing in step (2) is inoculated into a secondary culture medium for propagation culture. The secondary culture medium is prepared by adding 10 g of fine powder of cultivation material, 20 g of glucose, 1 g of fish peptone, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of the secondary culture medium. The culture medium is sterilized at 123° C. before inoculation. The fine powder of cultivation material is obtained by drying and grinding the residue of cultivation material after the fruiting of Tremella fuciformis cultivation. The propagation culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days to obtain liquid bacterial strains. The cultivation material is composed of 78% cottonseed hulls, 10% sawdust, 10% wheat bran, 1% glucose, and 1% light calcium carbonate.

[0036] The liquid spawn prepared in this example has a uniform brown-red color, a light wine aroma, small mycelium fragments, and is evenly distributed in the culture medium without floating or sinking. The golden ear fruiting bodies obtained by inoculating and cultivating the liquid spawn are brain-shaped, bright in color, orange-yellow or orange-red, and elastic to the touch. Each fruiting body weighs 193 g and has an average diameter of 11.1 cm.

Claims

1. A method for preparing a liquid bacterial strain of Tremella fuciformis in an industrial scale, characterized in that: The original species of golden ear mushroom are inoculated into a primary culture medium for conditioning culture to obtain a primary liquid strain, and then the liquid strain is expanded and cultured in a secondary culture medium. The primary culture medium is prepared by adding 30-35g of wheat, 10g of corn flour, 10-15g of glucose, 5g of maltose, 20-25mg of chitosan, 1.5-2g of magnesium sulfate and 3-4g of potassium dihydrogen phosphate into 1L of water.

2. The method for preparing a factory-based Tremella fuciformis liquid strain as claimed in claim 1, characterized in that: The golden ear mushroom seed is a golden ear fruiting body with a pink color and a cultivation period of 65 to 80 days. Specifically, a thin layer on the surface of the mushroom seed is selected as the original seed.

3. The method for preparing a factory-based Tremella fuciformis liquid strain as claimed in claim 1 or 2, characterized in that: The culture temperature is adjusted to 19-21° C., the culture speed is 150-250 rpm, and the culture period is 3-4 days.

4. A method for preparing a Tremella fuciformis liquid strain in an industrial scale according to any one of claims 1 to 3, characterized in that: The secondary culture medium used in the propagation culture is 1L of water with 10g of culture material powder, 20g of glucose, 1g of fish peptone, 1.5g of magnesium sulfate and 3g of potassium dihydrogen phosphate added thereto.

5. The method for preparing a factory-based Tremella fuciformis liquid strain as claimed in claim 4, characterized in that: The propagation culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days.

6. A method for preparing a liquid fungus strain of Tremella fuciformis in an industrial scale, characterized in that: The steps include: (1) Preparing stock Take golden ear fruiting bodies with pink color and cultivation period of 65-80 days as seed mushrooms, and pick a thin layer on the surface of the seed mushrooms as the original seed; (2) Conditioning and culture The original seed is inoculated into a primary culture medium for conditioning culture, wherein the primary culture medium is prepared by adding 30-35 g of wheat, 10 g of corn flour, 10-15 g of glucose, 5 g of maltose, 20-25 mg of chitosan, 1.5-2 g of magnesium sulfate, and 3-4 g of potassium dihydrogen phosphate into 1 L of water, and 4 pieces (1*1 cm in size) of the original seed are inoculated into 1 L of the primary culture medium, the culture temperature is adjusted to 19-21° C., the culture speed is 150-250 rpm, and the culture period is 3-4 days; (3) Propagation and cultivation The primary bacterial strain liquid obtained by conditioning culture in step (2) is inoculated into a secondary culture medium for propagation culture. The secondary culture medium is prepared by adding 10 g of culture medium powder, 20 g of glucose, 1 g of fish peptone, 1.5 g of magnesium sulfate, and 3 g of potassium dihydrogen phosphate to 1 L of the secondary culture medium. The culture medium powder is obtained by drying and grinding the culture medium residue after the fruiting of Tremella fuciformis culture. The propagation culture is carried out at a rotation speed of 600 pm and a temperature of 18-20° C. for 3 days.

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