Rodenticide containing tripterygium wilfordii and application of rodenticide

Abstract

The invention belongs to the field of agriculture, and relates to a rodenticide containing tripterygium wilfordii and application thereof, the rodenticide containing tripterygium wilfordii comprises the following components by weight: 0.1-0.5 mg of triptolide, 0.5-1.5 g of 3-methylbutyric acid, 0.5-1.5 g of ethyl acetate, 10-20 g of glucose, 60-100 g of peanut powder, 10-40 g of corn oil, 20-60 g of banana powder, and the balance of wheat flour. The rodenticide prepared by the invention has excellent luring and palatability to harmful rats, the feeding rate of the harmful rats can be improved, and the control effect of the rodenticide is remarkably improved; the compound can be used for preventing and treating harmful rats in fields such as farmland, indoor, forest or grassland.

Description

Technical Field

[0001] The invention belongs to the field of agriculture, and specifically relates to a rodenticide containing tripterygium wilfordii and application thereof. Background Art

[0002] The use of chemical agents is an important means of pest control, but long-term and large-scale use has led to problems of resistance, recurrence and residue. As a high-quality resource of green and environmentally friendly botanical rodenticide with multiple functions such as poisoning, anticoagulation and anti-fertility, Tripterygium wilfordii should be scientifically developed and utilized to provide safe agent selection and strategic support for pest control.

[0003] The rodenticide product registered by Jiangsu Wuxi Kailida Industrial Co., Ltd. (Pesticide Registration Certificate No.: PD20090004) is marketed as the only biological (plant-based) rodenticide registered in the fields of farmland, forest, grassland, public health, and residence.

[0004] Triptolide is a natural plant made from Tripterygium wilfordii, a botanical rodenticide. It is suitable for the taste of rodents, has a short-term killing effect and a dual effect of anti-fertility on male and female rodents (it can be used for emergency prevention and control, and can also be used for prevention). It will not cause the vigilance of rodents and cause super-compensatory reproduction and drug resistance, and can reduce the number and density of rodents, achieving a long-term rodent control effect.

[0005] In many methods of trapping and killing rodents, rice is often used as a drug carrier, but rodents tend to reject it, resulting in reduced control effectiveness. In order to find easy-to-use, cost-effective baits, the applicant selected different types of baits and screened out the best bait or bait combination to provide technical support for the further development of safe and efficient rodenticides. Summary of the invention

[0006] The object of the present invention is to provide a rodenticide containing Tripterygium wilfordii and its application. The specific technical scheme is as follows:

[0007] A rodenticide containing tripterygium wilfordii comprises the following ingredients by weight: 0.1-0.5 mg of tripterygium wilfordii, 0.5-1.5 g of 3-methylbutyric acid, 0.5-1.5 g of ethyl acetate, 10-20 g of glucose, 60-100 g of peanut powder, 10-40 g of corn oil, 20-60 g of banana powder, and wheat flour to make up to 1 kg.

[0008] Furthermore, it contains the following ingredients by weight: 0.2-0.3 mg of triptolide, 0.5-1.5 g of 3-methylbutyric acid, 0.5-1.5 g of ethyl acetate, 13-17 g of glucose, 70-90 g of peanut powder, 20-30 g of corn oil, 30-50 g of banana powder, and wheat flour to make up to 1 kg.

[0009] Furthermore, it contains the following ingredients by weight: 0.25 mg of triptolide, 1 g of 3-methylbutyric acid, 1 g of ethyl acetate, 15 g of glucose, 80 g of peanut powder, 25 g of corn oil, 40 g of banana powder, and wheat flour to make up to 1 kg.

[0010] The rodenticide of the present invention is used for controlling rodent pests, especially for controlling rodents in farmland, indoor, forest or grassland.

[0011] Compared with the prior art, the present invention achieves the following beneficial technical effects:

[0012] When triptolide is combined with the attractant ingredients 3-methylbutyric acid, ethyl acetate, glucose, peanut powder, corn oil and banana powder, the killing effect of rodenticide on harmful rodents can be significantly improved. DETAILED DESCRIPTION

[0013] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0014] The present invention is further explained below in conjunction with specific implementation modes.

[0015] Example 1: Test on the effectiveness of rodenticides in preventing grassland rodent pests

[0016] Experimental site: The experimental area is a grassland with flat terrain. The rodent pest species is plateau pika, and the rodent density is more than 72 / hm2.

[0017] Test drug:

[0018] Mouse poison 1: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg triptolide, 1 g 3-methylbutyric acid, 1 g ethyl acetate, 15 g glucose, 80 g peanut powder, 25 g corn oil, 40 g banana powder, wheat flour to make up 1 kg. Prepared by conventional wet granulation.

[0019] Rodent poison 2: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg triptolide, 2 g 3-methylbutyric acid, 15 g glucose, 80 g peanut powder, 25 g corn oil, 40 g banana powder, and wheat flour to make up to 1 kg.

[0020] Raticide 3: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg of triptolide, 2 g of ethyl acetate, 15 g of glucose, 80 g of peanut powder, 25 g of corn oil, 40 g of banana powder, and wheat flour to make up to 1 kg.

[0021] Rodent poison 4: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg of triptolide, 1 g of 3-methylbutyric acid, 1 g of ethyl acetate, 15 g of glucose, 120 g of peanut powder, 25 g of corn oil, and wheat flour to make up to 1 kg.

[0022] Rat poison 5: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg of triptolide, 80 g of peanut powder, 80 g of banana powder, and wheat flour to make up 1 kg.

[0023] Rodenticide 6: 0.25 mg / kg triptolide granules, containing the following ingredients by weight: 0.25 mg triptolide, 1 g 3-methylbutyric acid, 1 g ethyl acetate, 15 g glucose, 80 g soybean meal powder, 25 g corn oil, 40 g milk powder, and wheat flour to make up to 1 kg.

[0024] Rodenticide 7: Pesticide registration number: PD20090004.

[0025] Experimental method: Set up 5 dosing areas and 1 blank control area; each dosing area is 1hm2; set up 3 investigation plots of 0.15hm2 in each area; repeat the experiment 3 times. The experiment started to dosing before the grass turned green in spring, and the effective holes (holes with activity traces or fresh paw prints) were selected for dosing, and the dosage was 12g / hole.

[0026] Before applying the pesticide, investigate the effective holes and record the rat holes with signs of rat activities in the community as effective holes.

[0027] Palatability survey: 5 days after application, investigate the marked effective holes, record the consumption and remaining of rodenticides, and calculate the consumption rate.

[0028] Feeding rate (%) = feeding amount / total dosage × 100.

[0029] Effectiveness investigation: Investigation was conducted once every 30 days after medication, and at the same time as the control area.

[0030] Effective hole reduction rate (%) = (number of effective holes before prevention and control - number of effective holes after prevention and control) / number of effective holes before prevention and control × 100;

[0031] Corrected effective hole reduction rate (%) = (effective hole reduction rate in the control area - effective hole reduction rate in the control area) / 1 - effective hole reduction rate in the control area × 100;

[0032] Results and analysis:

[0033] As shown in Table 1, the feeding rate of rodenticide 3 and the existing rodenticide (pesticide registration certificate number: PD20090004) is lower, and the attraction and palatability to rodents are poor; compared with rodenticide 1 and rodenticides 2-6, it can be seen that the combination of attractant ingredients in rodenticides will have a significant effect on the feeding rate of rodenticides; when triptolide is combined with the attractant ingredients 3-methylbutyric acid, ethyl acetate, glucose, peanut powder, corn oil and banana powder, the attraction and palatability of rodenticides to rodents can be significantly improved, and their feeding rate can be increased.

[0034] Table 1 Rodenticide feeding rate

[0035] deal with Average feeding rate% Rat poison 1 92.2a Raticide 2 84.1b Raticide 3 80.3c Rat poison 4 83.9b Raticide 5 73.4d Raticide 6 84.0b Raticide 7 79.6c

[0036] As shown in Table 2, rodenticide 1 has an excellent control effect on plateau pikas, with a control effect of 97.6%; rodenticide 5 has a relatively low control effect on plateau pikas. Compared with rodenticides 2-6, it can be seen that when triptolide is combined with the attractant components 3-methylbutyric acid, ethyl acetate, glucose, peanut powder, corn oil and banana powder, the trapping effect of rodenticides on plateau pikas can be significantly improved.

[0037] Table 2 Effective hole prevention effect

[0038] deal with Corrected effective hole reduction rate (%) Rat poison 1 97.6 Raticide 2 88.3 Raticide 3 83.4 Rat poison 4 87.9 Raticide 5 78.5 Raticide 6 89.3 Raticide 7 81.7

[0039] Example 2: Test on the effectiveness of rodenticides in preventing farmland rodent pests

[0040] Experimental site: Wheat fields with high rat density and relatively consistent agronomic traits such as cultivation and management. The rodent pest species are Rattus norvegicus, Mus musculus, and Cricetulus hamster;

[0041] Test agent: Mouse agent 1-7 in Example 1

[0042] Test method: Set up 5 drug application areas and 1 blank control area; each area covers an area of ​​1hm2 and has 15 poison bait stations. A total of 75 poison bait stations are placed in the 5 areas, and 15g of poison bait is put into each poison bait station each time.

[0043] Investigation of control effect: The night trap method was used to investigate the control effect 2 days before the start of the experiment and 10 days after the application of the drug. 100 traps were set in each plot of farmland at a time, with a trap spacing of 5m and a row spacing of 50m. The traps were set before 18:00 in the evening, and the traps were collected after 6:00 the next morning for investigation. When the traps were collected, the number of effective mousetraps and the number of captured pests were recorded, and the reduction rate was calculated.

[0044] Results and analysis:

[0045] It can be seen from Table 3 that rodenticide 1 has an excellent control effect on farmland rodent pests, with a control efficiency of 91.7%; compared with rodenticides 2-6, it can be seen that when triptolide is combined with the attractant ingredients 3-methylbutyric acid, ethyl acetate, glucose, peanut powder, corn oil and banana powder, the killing effect of rodenticide on farmland rodent pests can be significantly improved.

[0046] Table 3: Effectiveness of rodenticides in preventing rodent infestation in farmland

[0047]

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit it.

Claims

1. A rodenticide containing Tripterygium wilfordii, characterized in that: The invention comprises the following ingredients by weight: 0.1-0.5 mg of triptolide, 0.5-1.5 g of 3-methylbutyric acid, 0.5-1.5 g of ethyl acetate, 10-20 g of glucose, 60-100 g of peanut powder, 10-40 g of corn oil, 20-60 g of banana powder and wheat flour to make up to 1 kg.

2. The rodenticide according to claim 1, characterized in that It contains the following ingredients by weight: 0.2-0.3 mg of triptolide, 0.5-1.5 g of 3-methylbutyric acid, 0.5-1.5 g of ethyl acetate, 13-17 g of glucose, 70-90 g of peanut powder, 20-30 g of corn oil, 30-50 g of banana powder, and wheat flour to make up 1 kg.

3. The rodenticide according to claim 2, characterized in that It contains the following ingredients by weight: 0.25 mg of triptolide, 1 g of 3-methylbutyric acid, 1 g of ethyl acetate, 15 g of glucose, 80 g of peanut powder, 25 g of corn oil, 40 g of banana powder, and wheat flour to make up 1 kg.

4. Use of the rodenticide according to any one of claims 1 to 3 for rodent control.

5. The use according to claim 4, characterized in that Used for the prevention and control of rodents in farmland, indoors, forests or grasslands.

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