Saussurea involucrata extract as well as preparation method and application thereof

Abstract

The invention relates to the technical field of skin care products, in particular to a saussurea involucrata extract and a preparation method and application thereof, and the preparation method of the saussurea involucrata extract comprises the following steps: redissolving and purifying an alcohol extract of saussurea involucrata through polyol and an adsorbent. According to the preparation method of the saussurea involucrata extract, the alcohol extract of the saussurea involucrata is redissolved and purified by adopting the polyhydric alcohol with small polarity and combining the adsorbent, the prepared saussurea involucrata extract is free of peculiar smell, light in color and free of microbial pollution problems, meanwhile, the biological activity is remarkably improved, and the product quality is remarkably improved; the application problem is solved, and the application of the composition in the field of cosmetics is greatly enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to a snow lotus extract and a preparation method and application thereof. Background Art

[0002] Snow lotus is a perennial herbaceous plant of the genus Saussurea in the Asteraceae family and is a precious plant raw material. The active ingredients in snow lotus include polymethoxylated flavonoids such as homoplanin, brown cyanidin, acacetin, etc., as well as flavonol glycoside compounds with quercetin as aglycone such as quercetin, isoquercetin, rutin, etc., especially rutin has the highest content. It also contains a rich variety of phenolic acid compounds such as chlorogenic acid, p-coumaric acid and dimer compounds with chlorogenic acid as the main body such as 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, etc., in addition to lignan compounds such as arctiin, which are the material basis for the biological activities of snow lotus extract such as whitening, anti-oxidation, anti-inflammatory, and anti-aging.

[0003] At present, the extract prepared by the extraction process of snow lotus often has problems such as unpleasant odor, dark color, few active ingredients, and no outstanding efficacy. Moreover, in the process of separation and enrichment of effective substances, the effective substances are lost to a large extent, resulting in a low content of effective substances in the product, which further affects the applicability and efficacy of snow lotus extract. Summary of the invention

[0004] One object of the present invention is to provide a method for preparing a snow lotus extract which can effectively increase the content of effective ingredients in the snow lotus extract and reduce the unpleasant odor and color of the snow lotus extract, comprising: re-dissolving and purifying the snow lotus alcohol extract through polyol and adsorbent.

[0005] In a specific embodiment of the present invention, the re-dissolution and purification process comprises: the ethanol extract of the snow lotus is re-dissolved in a polyol, mixed evenly with the adsorbent, and filtered.

[0006] In a specific embodiment of the present invention, the re-dissolution and purification process comprises: uniformly mixing the alcohol extract of the snow lotus, polyol and adsorbent, and filtering.

[0007] In a particular embodiment of the present invention, the adsorbent comprises a polar adsorbent.

[0008] In a specific embodiment of the present invention, the adsorbent includes at least one of silica gel, macroporous adsorption resin and neutral alumina.

[0009] In a specific embodiment of the present invention, the adsorbent comprises silica gel.

[0010] In a specific embodiment of the present invention, the amount of the adsorbent used is 0.05%-5% of the total mass of the polyol and the alcohol extract of snow lotus.

[0011] In a specific embodiment of the present invention, the amount of the adsorbent used is 0.5%-5% of the total mass of the polyol and the alcohol extract of snow lotus.

[0012] In a specific embodiment of the present invention, the amount of the adsorbent used is 1%-3% of the total mass of the polyol and the alcohol extract of snow lotus.

[0013] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-85°C.

[0014] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-50°C.

[0015] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-30°C.

[0016] In a specific embodiment of the present invention, the purification treatment time is 5-90 minutes.

[0017] In a specific embodiment of the present invention, the purification treatment time is 5-30 minutes.

[0018] In a specific embodiment of the present invention, the purification treatment time is 10-20 minutes.

[0019] In a specific embodiment of the present invention, the polyol includes at least one of 1,3-butanediol and dipropylene glycol.

[0020] In a specific embodiment of the present invention, the mass fraction of the polyol is ≥50%.

[0021] In a specific embodiment of the present invention, the mass fraction of the polyol is 50%-65%.

[0022] In a specific embodiment of the present invention, the mass percentage of the polyol is 100%.

[0023] In a specific embodiment of the present invention, the re-dissolving temperature is 20-85°C.

[0024] In a specific embodiment of the present invention, the re-dissolving temperature is 40-80°C.

[0025] In a specific embodiment of the present invention, the method for preparing the ethanol extract of snow lotus comprises: Snow lotus is extracted with ethanol, and the mass volume ratio of snow lotus to ethanol is 1:10-1:40; Extract at 45-85°C for 1-4h and filter.

[0026] In a specific embodiment of the present invention, the mass volume ratio of the snow lotus to ethanol is 1:15-1:30.

[0027] In a specific embodiment of the present invention, the mass volume ratio of the snow lotus to ethanol is 1:18-1:25.

[0028] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥70%.

[0029] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥90%.

[0030] In a specific embodiment of the present invention, the extraction temperature is 50-80°C.

[0031] In a specific embodiment of the present invention, the extraction time is 1-2h.

[0032] In a specific embodiment of the present invention, the method for preparing the ethanol extract of snow lotus further comprises: removing ethanol from the solution obtained after the filtration.

[0033] In a specific embodiment of the present invention, the ethanol in the solution obtained after the filtration is removed by concentration under reduced pressure.

[0034] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 38-55°C.

[0035] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 42-48°C.

[0036] In a specific embodiment of the present invention, the snow lotus is sterilized before being mixed with ethanol.

[0037] In a specific embodiment of the present invention, the sterilization treatment comprises high pressure steam sterilization.

[0038] In a specific embodiment of the present invention, the pressure of the high-pressure steam sterilization is 103.4-205.8 kPa.

[0039] In a specific embodiment of the present invention, the temperature of the high pressure steam sterilization is 120-132°C.

[0040] In a specific embodiment of the present invention, a snow lotus extract is also provided, which is prepared by the above preparation method.

[0041] In a specific embodiment of the present invention, there is also provided a use of a snow lotus extract in the preparation of cosmetics, wherein the snow lotus extract comprises the snow lotus extract prepared by the above preparation method and the above snow lotus extract.

[0042] Compared with the prior art, the present invention has the following beneficial effects: The use of polyol combined with an adsorbent to redissolve and purify the alcohol extract of snow lotus can effectively remove / dilute the unpleasant smell / odor of the obtained snow lotus extract, dilute the color of the snow lotus extract, and enhance its application in the field of cosmetics; The stability of the obtained snow lotus extract is further improved, and the content of the target ingredients is high with little loss, and it has significant anti-inflammatory, antioxidant and whitening effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0044] Figure 1 is the HPLC spectrum of the reconstituted solution of Example 1 of the present invention; Figure 2 The HPLC spectrum of the snow lotus extract of Example 1 of the present invention; Figure 3 is the HPLC spectrum of the reconstituted solution of Example 3 of the present invention; Figure 4 The HPLC spectrum of the snow lotus extract of Example 3 of the present invention; Figure 5 The snow lotus extracts and reconstituted solutions of Examples 1 and 3 of the present invention and the snow lotus extract of Comparative Example 1 show NO generation and cytotoxicity; Figure 6 This is a relationship diagram of the snow lotus extract and reconstituted solution of Examples 1 and 3 of the present invention, and the snow lotus extract of Comparative Example 1 to the amount of melanin produced. DETAILED DESCRIPTION

[0045] The technical scheme of the present invention will be described below in conjunction with the accompanying drawings and specific embodiments, but it will be appreciated by those skilled in the art that the following described embodiments are some embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work, all belong to the scope of protection of the present invention. If the specific conditions are not indicated in the embodiments, they are carried out according to the conditions recommended by the normal conditions or the manufacturers. If the manufacturer is not indicated in the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0046] In a specific embodiment of the present invention, a method for preparing a snow lotus extract is provided, comprising: the alcohol extract of the snow lotus is redissolved and purified by a polyol and an adsorbent. After the alcohol extract of the snow lotus is redissolved and purified by a polyol and an adsorbent, the obtained snow lotus extract has no obvious unpleasant odor, is light in color, has better stability, and has a high content of total flavonoids and total polyphenols in the product, and can more effectively exert whitening, anti-oxidation, anti-inflammatory and other effects.

[0047] Polyols are used in the re-dissolution and purification process, so that the active ingredients in the extract obtained after the snow lotus is extracted with an alcohol-containing solvent can be fully dissolved in the polyol, which is convenient for separating or enriching the active ingredients in the alcohol extract of the snow lotus.

[0048] The applicant found that the water-soluble components and their content in the alcohol extract of snow lotus have a great influence on the dark color and strong unpleasant odor of the snow lotus extract. The use of polyol combined with an adsorbent to redissolve and purify the alcohol extract of snow lotus can effectively adsorb the water-soluble components in the alcohol extract of snow lotus, thereby diluting the unpleasant odor and color of the obtained snow lotus extract. The obtained snow lotus extract retains the characteristic aroma and has almost no unpleasant odor.

[0049] Moreover, the adsorption treatment is carried out by directly mixing with an adsorbent. Compared with before the adsorbent treatment, the target component loss in the obtained snow lotus extract is very small, and the content change is extremely small, which is more conducive to the application of the snow lotus extract in creams, essential oils, toners, gels, essences, masks, sunscreens, lotions and other cosmetics. In addition, the above treatment method can also effectively remove the odor and polar macromolecules such as pigments in the alcohol extract of snow lotus, and can effectively improve the stability of the obtained snow lotus extract.

[0050] It is understandable that the alcohol extract of snow lotus can be an extract obtained by extracting and filtering snow lotus with an alcohol-containing solvent, or it can be a concentrate obtained by removing the alcohol-containing solvent from the above extract to obtain an extract or a crude drug, which can be adaptively adjusted according to actual needs. When the snow lotus extract is applied to the field of cosmetics or related fields, it can also be processed accordingly according to actual needs, such as sterilization.

[0051] In a specific embodiment of the present invention, the redissolution and purification treatment includes: the alcohol extract of snow lotus is redissolved in polyol, mixed evenly with the adsorbent, and filtered. The redissolution solution of the alcohol extract of snow lotus after redissolution in polyol is then mixed evenly with the adsorbent, and conventional mixing methods such as stirring can be used.

[0052] It is understandable that if the alcohol extract of snow lotus is a concentrate after the alcohol-containing solvent is removed, such as an extract state or a concentrate concentrated to the amount of crude drug, the alcohol extract of snow lotus can be mixed with polyols for redissolution; after complete redissolution, the redissolution solution obtained after the above redissolution is evenly mixed with the adsorbent, and after sufficient action, it is filtered to obtain a solution that is a snow lotus extract. Before mixing with the adsorbent, the mixed solution obtained after the alcohol extract of snow lotus is redissolved with polyols can also be filtered to remove insoluble matter, obtain a redissolution solution, and then mixed with the adsorbent.

[0053] If the alcohol extract of snow lotus is an extract from which the alcohol solvent has not been removed, the alcohol solvent can be removed in a conventional manner, and then mixed with a polyol for redissolution to obtain a redissolution solution; the above-mentioned extract can also be first mixed with a polyol, and then the alcohol solvent can be removed in a conventional manner such as concentration. For example, when the alcohol solvent is an ethanol aqueous solution, the extract obtained after the snow lotus is extracted with the ethanol aqueous solution is the alcohol extract of snow lotus, the above-mentioned extract is first mixed with a polyol, and then the ethanol is removed in a conventional manner such as concentration, and the obtained concentrate is redissolved with a polyol and filtered to obtain a redissolution solution. The above-mentioned redissolution solution is evenly mixed with the adsorbent, and after sufficient action, it is filtered, and the obtained filtrate is the snow lotus extract.

[0054] In a specific embodiment of the present invention, the re-dissolution and purification treatment includes: uniformly mixing the alcohol extract of snow lotus, polyol and adsorbent, and filtering. It is understandable that if the alcohol extract of snow lotus is a concentrate after removing the alcohol-containing solvent, such as an extract state or a concentrate concentrated to the amount of crude drug, the alcohol extract of snow lotus, polyol and adsorbent can be directly mixed uniformly, and after sufficient action, filtered, and the resulting solution is the snow lotus extract.

[0055] If the alcohol extract of snow lotus is an extract from which the alcohol solvent has not been removed, the alcohol solvent can be removed by conventional methods such as concentration, and then the extract is mixed with a polyol and an adsorbent, and after sufficient reaction, it is filtered, and the resulting solution is the snow lotus extract. The above-mentioned extract can also be first mixed with a polyol and an adsorbent, and then the alcohol solvent can be removed by conventional methods such as concentration, and then after sufficient reaction, it is filtered, and the resulting solution is the snow lotus extract.

[0056] In a particular embodiment of the invention, the adsorbent comprises a polar adsorbent.

[0057] In a specific embodiment of the present invention, the adsorbent includes at least one of silica gel, macroporous adsorption resin and neutral alumina. The adsorbent is silica gel, macroporous adsorption resin or neutral alumina, etc., which can further improve the adsorption treatment effect and reduce the loss of target components in the product. Among them, the polar macroporous adsorption resin can be a macroporous adsorption resin such as D101, AB-8, HP-100. It can be understood that the adsorbent is usually in a powder state, and its particle size may not be specifically limited, and commercially available conventional particle size is also acceptable.

[0058] In a specific embodiment of the present invention, the adsorbent may further preferably be silica gel.

[0059] In a specific embodiment of the present invention, the amount of the adsorbent is 0.05%-5% of the total mass of the polyol and the alcohol extract of snow lotus. The amount of the adsorbent is kept within a suitable range, which can effectively improve the adsorption efficiency and adsorption effect.

[0060] It is understandable that the amount of adsorbent used can also be 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. of the total mass of the polyol and the alcohol extract of snow lotus, or an amount between any two of the above values.

[0061] In a specific embodiment of the present invention, the amount of the adsorbent used is 0.5%-5% of the total mass of the polyol and the alcohol extract of snow lotus.

[0062] In a specific embodiment of the present invention, the amount of the adsorbent used is 1%-3% of the total mass of the polyol and the alcohol extract of snow lotus.

[0063] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-85° C. It is understood that when the alcohol extract of snow lotus, polyol and adsorbent are mixed, redissolved and purified, the temperature of the purification treatment is the same as the temperature of the redissolution. When the alcohol extract of snow lotus is first redissolved in polyol and then mixed with adsorbent for purification, the temperature of the purification treatment may be the same as or different from the temperature of the redissolution.

[0064] The temperature of the purification treatment may be 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or 85° C., or a temperature between any two of the above values.

[0065] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-50° C. When the alcohol extract of snow lotus is first redissolved in a polyol and then mixed with an adsorbent for purification treatment, the temperature of the purification treatment is preferably 20-50° C. The temperature of the purification treatment is carried out at a lower temperature, which can not only effectively interact with the adsorbent and the redissolved solution, but also save costs.

[0066] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-30°C.

[0067] In a specific embodiment of the present invention, the time of purification treatment is 5-90min. It is understandable that when the alcohol extract of snow lotus, polyol and adsorbent are mixed, redissolved and purified, the time of purification treatment is the same as the time of redissolved. When the alcohol extract of snow lotus is first redissolved by polyol and then mixed with adsorbent for purification treatment, the time of purification treatment can be the same as the time when redissolved, or it can be different.

[0068] The purification treatment time can be 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90 minutes, or it can be a time between any two of the above values.

[0069] In a specific embodiment of the present invention, the purification treatment time is 5-30 minutes.

[0070] In a specific embodiment of the present invention, the purification treatment time is 10-20 minutes.

[0071] In a specific embodiment of the present invention, the polyol includes at least one of 1,3-butanediol and dipropylene glycol.

[0072] In a specific embodiment of the present invention, the mass fraction of the polyol is ≥50%. It is understood that the polyol can be a polyol solution with a mass fraction of 100%, or a polyol aqueous solution; when the polyol is a polyol aqueous solution, the mass fraction of the polyol aqueous solution is ≥50%.

[0073] The use of a higher mass fraction of polyols can more effectively improve the re-dissolution efficiency of the snow lotus alcohol extract, further reduce the loss of the target component, and achieve the purpose of removing / diluting the unpleasant odor and color of the snow lotus extract, which is conducive to forming a stable system.

[0074] In a specific embodiment of the present invention, the mass fraction of the polyol is 50%-65%. When the polyol is a polyol aqueous solution, the mass fraction of the polyol is within this range, which can be more conducive to the redissolution of the alcohol extract of snow lotus, obtain a more stable redissolution solution, and simultaneously achieve the purpose of removing / diluting unpleasant odors and diluting product color. The mass fraction of the polyol can be further preferably 50%-60%.

[0075] In a specific embodiment of the present invention, the mass fraction of the polyol is 100%. When the polyol adopts a polyol solution with a mass fraction of 100%, the alcohol extract of snow lotus can be more fully redissolved in the polyol, the loss of the target component can be reduced, and a more stable redissolved solution can be obtained; and when the alcohol extract of snow lotus is redissolved with a mass fraction of 100% polyol, the unpleasant smell of the obtained snow lotus extract can also be effectively removed / diluted, and the color of the snow lotus extract can be diluted.

[0076] In a specific embodiment of the present invention, during the redissolution and purification process, the mass ratio of the alcohol extract of snow lotus to the polyol is 1:2-1:50. It is understood that the mass ratio of the alcohol extract of snow lotus to the polyol can be specifically adjusted according to actual needs, and the preferred range can be selected from the above range.

[0077] It is understood that if the alcohol extract of snow lotus is a concentrate after the alcohol-containing solvent is removed, the mass ratio of the alcohol extract of snow lotus to the polyol may preferably be within the above range. If the alcohol extract of snow lotus is an extract from which the alcohol-containing solvent has not been removed, the alcohol-containing solvent may be removed by a conventional treatment method and then mixed with the polyol. At this time, the mass ratio between the concentrate after the alcohol-containing solvent is removed and the polyol is preferably maintained within the above range.

[0078] In a specific embodiment of the present invention, during the redissolution and purification treatment, the mass ratio of the ethanol extract of snow lotus to the polyol is 1:4-1:30.

[0079] In a specific embodiment of the present invention, the temperature of the reconstitution is 20-85° C. Similarly, it is understood that when the alcohol extract of snow lotus, polyol and adsorbent are mixed and reconstituted and purified, the temperature of the reconstitution is the same as the temperature of the purification process.

[0080] When the alcohol extract of snow lotus is first reconstituted with a polyol and then mixed with an adsorbent for purification, the temperature of the reconstitution may be the same as or different from the temperature of the purification treatment. In addition, under this treatment method, the time of the reconstitution may be the same as or different from the time of the purification treatment. The reconstitution time may be adaptively adjusted according to the reconstitution situation until the alcohol extract of snow lotus is fully reconstituted, which can usually be processed for 0.5-2 hours.

[0081] The re-dissolution temperature may be 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or 85° C., or a temperature between any two of the above values.

[0082] In a specific embodiment of the present invention, the temperature for redissolution is 40-80°C. Redissolution using a relatively high temperature treatment can be beneficial to improving the efficiency of redissolution. It is understandable that when the alcohol extract of snow lotus is first redissolved with a polyol and then mixed with an adsorbent for purification treatment, the temperature for redissolution may preferably be 40-80°C; the temperature for purification treatment may preferably be 20-50°C. When the alcohol extract of snow lotus is first redissolved with a polyol and then mixed with an adsorbent for purification treatment, the time for redissolution may preferably be 40-90min; the time for purification treatment may preferably be 5-30min.

[0083] In a specific embodiment of the present invention, the ethanol extract of snow lotus can be prepared or a commercial product.

[0084] In a specific embodiment of the present invention, the preparation method of the alcohol extract of snow lotus comprises: extracting the snow lotus with ethanol, the mass volume ratio of the snow lotus to ethanol is 1:10-1:40; extracting at 45-85°C for 1-4h, and filtering.

[0085] Snow lotus can be a species of the genus Saussurea of ​​the family Asteraceae ( Saussurea DC.) plants. The snow lotus used to prepare the ethanol extract of snow lotus can be the whole plant of snow lotus; it can also be the above-ground part of snow lotus, such as the flower of snow lotus, or the leaf of snow lotus, or the flower and leaf of snow lotus; it can also be the underground part of snow lotus, such as the root of snow lotus, etc.; preferably, the whole plant of snow lotus is used.

[0086] It is understood that when using ethanol to extract snow lotus, the number of extractions can be one or more times, and can be adaptively adjusted according to the actual extraction situation. Among them, the extraction time of 1-4 hours at 45-85°C is the time range for a single extraction. When multiple extractions are performed, the time of any single extraction can be in the range of 1-4 hours.

[0087] For example, when performing two extractions, the snow lotus can be mixed with a certain amount of ethanol, extracted for 3 hours at a certain temperature, and filtered to obtain a first filter residue and a first filtrate; the first filter residue is mixed with a certain amount of ethanol, extracted for 3 hours at a certain temperature, filtered to obtain a second filtrate, and the first filtrate and the second filtrate are combined to obtain an alcohol extract of the snow lotus. It should be noted that when multiple extractions are performed, the conditions (such as solid-liquid ratio, temperature, extraction time, etc.) for each extraction can be the same or different.

[0088] In a specific embodiment of the present invention, the mass volume ratio of snow lotus to ethanol is 1:15-1:30.

[0089] In a specific embodiment of the present invention, the mass volume ratio of snow lotus to ethanol is 1:18-1:25.

[0090] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥70%. Ethanol can be an ethanol solution with a mass fraction of 100%, or it can be an ethanol aqueous solution. When ethanol is an ethanol aqueous solution, the mass fraction of the ethanol aqueous solution is ≥70%. Using ethanol with a higher mass fraction as an extraction solvent can effectively reduce the leaching of water-soluble substances in snow lotus, improve the leaching of target components, and help to dilute the unpleasant smell and color of the obtained snow lotus extract. Wherein, ethanol can also be used to soak the snow lotus for a certain time before extracting the snow lotus, and the soaking time can be appropriately selected according to demand.

[0091] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥90%.

[0092] In a specific embodiment of the present invention, the extraction temperature may preferably be 50-80°C.

[0093] In a specific embodiment of the present invention, the extraction time may preferably be 1-2 hours.

[0094] In a specific embodiment of the present invention, the method for preparing the ethanol extract of snow lotus further comprises: after the snow lotus is extracted with ethanol, removing the ethanol from the solution obtained after filtering.

[0095] In a specific embodiment of the present invention, the ethanol in the solution obtained after filtration is removed by concentration under reduced pressure.

[0096] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 38-55°C. The reduced pressure concentration treatment at a relatively low temperature can avoid the color of the obtained product from deepening while removing the alcohol-containing solvent ethanol, which is beneficial to further dilute the color of the snow lotus extract. The solution obtained after ethanol extraction and filtration is subjected to reduced pressure concentration treatment, which can be concentrated to an extract state, or to a mass approximately equal to the amount of crude drug.

[0097] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 42-48°C.

[0098] In a specific embodiment of the present invention, the snow lotus may be crushed before being mixed with ethanol to improve the extraction efficiency.

[0099] In a specific embodiment of the present invention, the snow lotus may be sterilized before being mixed with ethanol.

[0100] In a specific embodiment of the present invention, the sterilization treatment includes high-pressure steam sterilization. Before the snow lotus is extracted with ethanol, the snow lotus is first subjected to high-pressure steam sterilization treatment, which can not only effectively solve the problem of microbial residues, but also destroy the tissue structure of the snow lotus raw material and improve the dissolution efficiency of the active ingredients in the snow lotus.

[0101] In a specific embodiment of the present invention, the pressure of high pressure steam sterilization is 103.4-205.8 kPa.

[0102] In a specific embodiment of the present invention, the temperature of high pressure steam sterilization is 120-132° C. It is understandable that the time of high pressure steam sterilization can be adaptively adjusted according to actual conditions, for example, it can be 10-50 minutes.

[0103] In a specific embodiment of the present invention, a snow lotus extract is also provided, which is prepared by the above method.

[0104] In a specific embodiment of the present invention, the mass ratio of total polyphenols to total flavonoids in the snow lotus extract is (0.5-1.2): (1.5-6); the above ratio is further preferably (0.7-1): (2-5), and the above ratio is further preferably (0.79-0.95): (3.2-4). The mass ratio of total polyphenols to total flavonoids in the snow lotus extract is maintained within the above range, so that it has good anti-inflammatory, whitening and antioxidant effects.

[0105] In a specific embodiment of the present invention, the snow lotus extract comprises chlorogenic acid, rutin, 1,5-dicaffeoylquinic acid and arctiin.

[0106] In a specific embodiment of the present invention, there is also provided a use of a snow lotus extract in the preparation of cosmetics, wherein the snow lotus extract is the above-mentioned snow lotus extract or the snow lotus extract prepared by the above-mentioned preparation method.

[0107] The following is further described using specific embodiments and experimental examples.

[0108] Example 1 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus was added to 2000 mL of 95% ethanol and soaked for 0.5 h; then the temperature was raised to 75 °C and extracted for 2 h, and filtered to obtain solution A and filter residue; 2) The filter residue was added to 2000 mL of 95% ethanol again and extracted at 75°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure and evaporated to the amount of crude drug at 45°C to obtain the alcohol extract of snow lotus; 3) Mix the ethanol extract of snow lotus with 100% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 70°C for 1 hour, cool to room temperature, and filter to obtain a redissolved solution; 4) Mixing the reconstituted solution with silica gel powder, stirring at room temperature for 15 minutes, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of silica gel powder is 0.05% of the mass of the reconstituted solution.

[0109] Example 2 The preparation method of snow lotus extract comprises: 1) 100g of dried whole plant of snow lotus, without sieving the coarse powder, was sterilized at 103.4Kpa and 121.3℃ for 20min; 2) Then add it into 2000mL 95% ethanol and soak it for 0.5h; then heat it to 75℃ and extract it for 2h, filter it and obtain solution A and filter residue; 3) The filter residue was added to 2000 mL of 95% ethanol again and extracted at 75°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure at 48°C and evaporated to the amount of crude drug, to obtain the ethanol extract of snow lotus; 4) Mix the alcohol extract of snow lotus with 100% dipropylene glycol in a mass ratio of 1:30, stir and redissolve at 70°C for 1 hour, cool to room temperature, and filter to obtain a redissolved solution; 5) Mix the reconstituted solution with silica gel powder, stir at 40°C for 10 min, filter, and sterilize to obtain the snow lotus extract; the amount of silica gel powder is 0.5% of the mass of the reconstituted solution.

[0110] Example 3 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus was added to 2000 mL of 70% ethanol and soaked for 0.5 h; then the temperature was raised to 75 °C for extraction for 1 h, and filtered to obtain solution A and filter residue; 2) The filter residue was added to 2000 mL of 70% ethanol again and extracted at 75°C for 1 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure and rotary evaporated at 55°C to the amount of crude drug, to obtain the ethanol extract of snow lotus; 3) Mix the ethanol extract of snow lotus with 80% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 85°C for 30 minutes, cool to room temperature, and filter to obtain a redissolved solution; 4) Mixing the reconstituted solution with silica gel powder, stirring at 50° C. for 5 min, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of silica gel powder is 3% of the mass of the reconstituted solution.

[0111] Example 4 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus, without sieving the coarse powder, was sterilized at 205.8 KPa and 132 °C for 10 min; 2) Then add it into 2000mL 70% ethanol and soak it for 0.5h; then heat it to 50℃ and extract it for 1h, filter it and obtain solution A and filter residue; 3) The filter residue was added into 2000 mL 70% ethanol again, extracted at 50°C for 1 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure at 45°C and evaporated to the amount of crude drug to obtain the ethanol extract of snow lotus. 4) Mix the ethanol extract of snow lotus with 50% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 70°C for 30 minutes, cool to room temperature, and filter to obtain a redissolved solution; 5) Mixing the reconstituted solution with silica gel powder, stirring at 20° C. for 30 min, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of silica gel powder is 3% of the mass of the reconstituted solution.

[0112] Example 5 The preparation method of snow lotus extract comprises: 1) 100g of dried whole plant of snow lotus, without sieving the coarse powder, was sterilized at 121.3Kpa and 121.3℃ for 20min; 2) Then add 1500mL 70% ethanol and soak for 0.5h; then heat to 50℃ and extract for 2h, filter to obtain solution A and filter residue; 3) The filter residue was added to 1500 mL of 70% ethanol again, extracted at 50°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure at 55°C and evaporated to the amount of crude drug to obtain the ethanol extract of snow lotus. 4) Mix the ethanol extract of snow lotus with 60% 1,3-butanediol in a mass ratio of 1:2, stir and redissolve at 85°C for 30 minutes, cool to room temperature, and filter to obtain a redissolved solution; 5) Mix the reconstituted solution with D101 macroporous adsorption resin, stir at 50°C for 15 min, filter, and sterilize to obtain the snow lotus extract; wherein the amount of D101 macroporous adsorption resin is 5% of the mass of the reconstituted solution.

[0113] Example 6 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus was added to 3000 mL of 95% ethanol and soaked for 0.5 h; then the temperature was raised to 75 °C and extracted for 2 h, and filtered to obtain solution A and filter residue; 2) The filter residue was added to 3000 mL of 95% ethanol again and extracted at 75°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure and evaporated to the amount of crude drug at 45°C to obtain the ethanol extract of snow lotus; 3) Mix the alcohol extract of snow lotus, 100% 1,3-butanediol and silica gel powder, stir and dissolve and purify at 70°C for 1 hour, cool to room temperature, filter and sterilize to obtain the snow lotus extract; wherein the mass ratio of the alcohol extract of snow lotus to 1,3-butanediol is 1:4, and the amount of silica gel powder is 2% of the total mass of the alcohol extract of snow lotus and 1,3-butanediol.

[0114] Example 7 The preparation method of snow lotus extract comprises: 1) The commercially available (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd.) ethanol extract of snow lotus in extract form was mixed with 100% 1,3-butanediol in a mass ratio of 1:4, stirred and re-dissolved at 80°C for 1 hour, cooled to room temperature, and filtered to obtain a re-dissolved solution; 2) Mixing the reconstituted solution with silica gel powder, stirring at 25° C. for 20 min, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of silica gel powder is 3% of the mass of the reconstituted solution.

[0115] Example 8 The preparation method of snow lotus extract comprises: 1) 100g of dried whole plant of snow lotus, without sieving the coarse powder, was added into 2000mL 95% ethanol and soaked for 0.5h; then the temperature was raised to 75℃ for extraction for 2h, filtered to obtain solution A; solution A was concentrated under reduced pressure and rotary evaporated at 45℃ to the amount of crude drug, to obtain the ethanol extract of snow lotus; 2) Mix the ethanol extract of snow lotus with 100% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 70°C for 1 hour, cool to room temperature, and filter to obtain a redissolved solution; 3) Mixing the reconstituted solution with silica gel powder, stirring at room temperature for 15 minutes, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of silica gel powder is 1% of the mass of the reconstituted solution.

[0116] Comparative Example 1 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus was added to 2000 mL of 70% ethanol, the temperature was raised to 80°C, and the mixture was extracted for 2 h. The mixture was filtered to obtain solution A and residue. 2) The residue was added to 2000 mL of 50% ethanol again and extracted at 85°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure at 70°C and evaporated to the amount of crude drug, to obtain the ethanol extract of snow lotus; 3) The ethanol extract of snow lotus was mixed with 50% 1,3-butanediol in a mass ratio of 1:4, stirred and redissolved at 50° C. for 60 min, cooled to room temperature, filtered, and sterilized to obtain the snow lotus extract.

[0117] Comparative Example 2 The preparation method of snow lotus extract comprises: 1) 100 g of dried whole plant of snow lotus was added to 2000 mL of 95% ethanol and soaked for 0.5 h; then the temperature was raised to 75 °C and extracted for 2 h, and filtered to obtain solution A and filter residue; 2) The filter residue was added to 2000 mL of 95% ethanol again and extracted at 75°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure and evaporated to the amount of crude drug at 45°C to obtain the alcohol extract of snow lotus; 3) Mix the alcohol extract of snow lotus with 100% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 70°C for 1 hour, cool to room temperature, and filter to obtain a redissolved solution; 4) Mixing the reconstituted solution with β-cyclodextrin, stirring at room temperature for 15 minutes, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of β-cyclodextrin is 1% of the reconstituted solution.

[0118] Comparative Example 3 The preparation method of snow lotus extract comprises: 1) Sterilize 100g of dried whole plant of snow lotus, without sieving the coarse powder, at 205.8Kpa and 132℃ for 10min; 2) Then add 1500mL 70% ethanol and soak for 0.5h, then heat to 45℃ and extract for 1h, filter to obtain solution A and filter residue; 3) The residue was added to 1500 mL of 70% ethanol again and extracted at 75°C for 1 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure and evaporated to the amount of crude drug at 55°C to obtain the alcohol extract of snow lotus; 4) Mix the ethanol extract of snow lotus with 80% 1,3-butanediol in a mass ratio of 1:4, stir and redissolve at 85°C for 30 minutes, cool to room temperature, and filter to obtain a redissolved solution; 5) Mixing the reconstituted solution with activated clay, stirring at 50° C. for 5 min, filtering, and sterilizing to obtain the snow lotus extract; wherein the amount of activated clay is 5% of the mass of the reconstituted solution.

[0119] Comparative Example 4 The preparation method of snow lotus extract comprises: 1) 100g of dried whole plant of snow lotus, without sieving the coarse powder, was sterilized at 121.3Kpa and 121.3℃ for 20min; 2) Then add it into 2000mL 70% ethanol and soak it for 0.5h; then heat it to 50℃ and extract it for 2h, filter it and obtain solution A and filter residue; 3) The filter residue was added to 2000 mL of 70% ethanol again, extracted at 50°C for 2 h, filtered to obtain solution B, and solutions A and B were combined, concentrated under reduced pressure at 55°C and evaporated to the amount of crude drug to obtain the ethanol extract of snow lotus. 4) Mix the ethanol extract of snow lotus with water in a mass ratio of 1:2, stir and redissolve at 70°C for 30 minutes, cool to room temperature, and filter to obtain a redissolved solution; 5) Mix the reconstituted solution with D101 macroporous adsorption resin, stir at 50°C for 15 min, and filter to obtain solution C; wherein the amount of D101 macroporous adsorption resin is 5% of the mass of the reconstituted solution; 6) Solution C was mixed with 60% 1,3-butanediol in a mass ratio of 1:2, and the mixture was sterilized to obtain the snow lotus extract.

[0120] When the snow lotus extract was prepared by the method of this comparative example, precipitation phenomenon was obvious.

[0121] Experimental Example 1 Product Odor Evaluation 21 volunteers were recruited to evaluate the odor of the products prepared in Examples 1-8 and Comparative Examples 1-4. The products were scored based on their characteristic odor, the presence or absence of unpleasant odor / smell, the degree of acceptance of the product odor, and the degree of liking for the odor. Each indicator was scored to a full score of 10, and the average value was taken for comparison. The evaluation results of the use effects of the embodiments are shown in Table 1.

[0122] Table 1 Odor evaluation table of the products obtained from Examples 1-8 and Comparative Examples 1-4 Category Characteristic smell Odor degree Acceptance Like Example 1 Snow Lotus Extract 10 0 10 9 Example 2 Snow lotus extract 10 1 10 9 Example 3 Snow lotus extract 8 3 8 8 Example 3 Reconstitution Solution 9 7 5 5 Example 4 Snow Lotus Extract 9 2 9 9 Example 5 Snow Lotus Extract 7 4 8 8 Example 6 Snow Lotus Extract 10 0 10 9 Example 7 Snow Lotus Extract 9 2 9 8 Example 8 Snow Lotus Extract 8 3 8 7 Comparative Example 1 Snow Lotus Extract 3 8 2 2 Comparative Example 2 Snow Lotus Extract 4 7 6 4 Comparative Example 3 Snow Lotus Extract 3 8 3 2 Comparative Example 4 Snow Lotus Extract 2 9 2 1 It can be seen from the data in Table 1 that among Examples 1-5, the snow lotus extract prepared by the method of Example 1 has the highest evaluation, that is, it highly retains the characteristic smell of snow lotus flowers and has no odor, and is widely loved by volunteers. However, the products that have not been treated with silica gel (the reconstituted solution of Example 3 and the snow lotus extract prepared by the method of Comparative Example 1) have odor substances that are found by most subjects. Therefore, after silica gel solid phase extraction, the odor of the snow lotus extract can be significantly reduced and the quality of the snow lotus extract can be improved.

[0123] For the snow lotus extracts obtained in Comparative Examples 1-4, the unpleasant odor of the product cannot be effectively reduced, and the loss of the target component is relatively large.

[0124] Experimental Example 2 The total polyphenol and total flavonoid contents of the samples obtained in Examples 1-5 and Comparative Examples 1-4 were tested using the following method.

[0125] Detection of total polyphenol content: Add 100 µL of the prepared gallic acid standard solution of each concentration and each sample, and then add 900 µL of deionized water to make the volume 1 mL; Then, 100 μL of Folin phenol reagent was added and allowed to react for 3 min; After adding 200µL of 7% sodium carbonate solution, add 700µL of deionized water to make the volume 2mL. After reacting for 1 h, 200 µL of the reaction solution was pipetted into a 96-well and the absorbance was measured at 700 nm using an ELISA reader; The total polyphenol content in the sample was calculated using the prepared gallic acid standard curve and the absorbance of the sample.

[0126] Detection of total flavonoids content: Add 100 µL of the prepared rutin standard solution of each concentration and each sample, and then add 300 µL of 95% ethanol; Then, 20 µL of 10% Al(NO3)3 solution was added and allowed to react for 3 min (Blank: replaced with deionized water); After adding 20µL of 1mol / L sodium acetate solution, add 560µL of deionized water to make the volume 1mL. After reacting for 30 min, 200 µL of the reaction solution was pipetted into a 96-well and the absorbance was measured at 415 nm using an ELISA reader; The total flavonoid content in the sample was calculated using the rutin standard curve and the absorbance of the sample.

[0127] The total polyphenol and total flavonoid contents in the snow lotus extracts obtained in Examples 1-5 and Comparative Examples 1-4 and the reconstituted solutions in Examples 1 and 3 that were not treated with silica gel are shown in Table 2.

[0128] Table 2 Total polyphenol and total flavonoid contents in the products obtained from Examples 1-5 and Comparative Examples 1-4 Category Total polyphenol content (mg / mL) Total flavonoids content (mg / mL) Example 1 Reconstitution Solution 0.95 4.0 Example 1 Snow Lotus Extract 0.93 3.8 Example 2 Snow lotus extract 0.89 3.6 Example 3 Reconstitution Solution 0.91 4.0 Example 3 Snow lotus extract 0.86 3.2 Example 4 Snow Lotus Extract 0.82 3.7 Example 5 Snow Lotus Extract 0.79 3.5 Comparative Example 1 Snow Lotus Extract 0.92 4.2 Comparative Example 2 Snow Lotus Extract 0.42 1.6 Comparative Example 3 Snow Lotus Extract 0.35 1.2 Comparative Example 4 Snow Lotus Extract 0.46 1.7 It can be seen from the data in Table 2 that the snow lotus extracts prepared in Examples 1-5 have high contents of total polyphenols and flavonoids, indicating that the snow lotus extracts prepared by this method can better enrich the polyphenol and flavonoid chemical components, have strong antioxidant activity, and the phenolic hydroxyl groups contained therein can have a strong scavenging effect on free radicals through electron transfer or hydrogen donation.

[0129] Compared with the product (redissolved solution) not treated with silica gel, although the total polyphenol and flavonoid contents in the snow lotus extract obtained after silica gel treatment in Examples 1 and 3 decreased slightly, the decrease was very small. However, the total polyphenol and flavonoid contents in the snow lotus extract obtained after treatment with reagents such as β-cyclodextrin (Comparative Example 2) and activated white clay (Comparative Example 3) decreased significantly, respectively, by half. Therefore, selecting a specific adsorbent can not only effectively dilute unpleasant odors and colors, but also effectively reduce the loss of target components in the product.

[0130] Experimental Example 3 Stability Observation Data The stability observation data of the products obtained in Example 1, Example 3 and Comparative Example 1 are shown in Table 3.

[0131] Table 3 Stability observation data of the products obtained in Example 1, Example 3 and Comparative Example 1 Detection indicators Example 1 Reconstitution Solution Example 1 Snow Lotus Extract Example 3 Reconstitution Solution Example 3 Snow lotus extract Comparative Example 1 Snow Lotus Extract Precipitation or not (Day 0) none none none none none Precipitation (room temperature, day 3) none none none none have Precipitation (room temperature, 45th day) none none have none have Color (room temperature, day 0) yellow Light yellow Brown yellow Dark Brown Color (room temperature, 45 days) Dark yellow Light yellow Reddish brown yellow Dark Brown The reconstituted solution prepared by the method of Example 3 and the snow lotus extract prepared by Comparative Example 1 have obvious precipitation phenomenon. In addition, it can be found from Table 3 that the stability of the snow lotus extract obtained after the reconstituted solution of Example 3 is treated with silica gel is significantly improved, and it is still a homogeneous stable system after storage for 45 days; and, after silica gel treatment, the color of the snow lotus extract of Example 3 changes from brown to yellow, and the color becomes significantly lighter. Compared with the reconstituted solution before silica gel treatment, the snow lotus extract prepared by the preparation method of Example 1 also becomes lighter in color to a certain extent, from yellow to light yellow; and, after being stored at room temperature for 45 days, the color of the reconstituted solution before silica gel treatment has a slight darkening, from yellow to dark yellow, while the color of the snow lotus extract obtained after silica gel treatment has almost no change. It can be seen that silica gel adsorption treatment can effectively dilute the color of the snow lotus extract and improve the stability of the snow lotus extract; for products that are unstable by themselves, after adding silica gel adsorption treatment, the instability of the product can be effectively improved, and the product stability and formula applicability can be significantly improved.

[0132] Experimental Example 4 High Performance Liquid Chromatography (HPLC) Material Composition Analysis HPLC material composition analysis method: The snow lotus extract and the reconstituted solution obtained by the preparation methods of Example 1 and Example 3 were diluted to 10% with methanol, and then 1.5 mL was drawn with a syringe and passed through an organic 0.22 micron filter membrane and injected into a sample bottle as a sample for later use; HPLC analysis of snow lotus extract and reconstituted solution was performed using an Agilent system, e2695 PAD UV detector, Eclipse XBD C18 (5 µm, 4.6 mm × 250 mm) column, and mobile phases: A: water (0.1% acetic acid); B: acetonitrile. The injection volume was 5 µL, and the detection wavelength was 280 nm.

[0133] See also Figure 1-4 By performing HPLC material composition analysis on the snow lotus extracts obtained in Examples 1 and 3 and the reconstituted solutions of the products that have not been treated with silica gel, it was found that the snow lotus extracts prepared by this method mainly include biologically active ingredients such as chlorogenic acid, rutin, 1,5-dicaffeoylquinic acid and arctiin, which are the material basis for the snow lotus flower extract to exert its antioxidant, anti-inflammatory and whitening effects.

[0134] The relative content ratios of chlorogenic acid, rutin, 1,5-dicaffeoylquinic acid and arctiin in the product can be obtained from the HPLC test data, as shown in Table 4. As can be seen from Table 4, the chlorogenic acid content is the richest in the snow lotus extract prepared by this method, and the relative content of the target component changes little before and after silica gel treatment, which hardly affects the biological activity of the product.

[0135] Moreover, compared with the reconstituted solution without silica gel treatment, the relative contents of chlorogenic acid and 1,5-dicaffeoylquinic acid in the snow lotus extract obtained after silica gel treatment decreased slightly, while the relative contents of rutin and arctiin increased slightly. It can be seen that the purification treatment of the present invention has a certain selective enrichment effect on the target component.

[0136] Table 4 Composition and proportion of samples obtained in Examples 1 and 3

[0137] Experimental Example 5 Evaluation of anti-inflammatory activity The anti-inflammatory activity was evaluated by the NO generation inhibition activity method. The specific determination experimental method is as follows: The prepared snow lotus extract / reconstituted solution stock solution was used as a diluent to prepare three material solutions of 0.1%, 0.2% and 0.4% respectively. The cells were mouse macrophage RAW264.7 cells. On the first day, the density of the cells was 2×10^5 cell / well in 24 wells. After 18 hours, the old culture medium solution was removed, and 1 mL of DMEM cell culture medium containing 1ug / mL lipopolysaccharide (LPS) was added to each well. Then, the prepared product solutions were added. After culturing in the incubator for 24 hours, 100μL of the supernatant was aspirated from each well, and then an equal volume of Griess reagent was added to determine the NO content. Then, the absorbance A was measured at a wavelength of 540nm, and the NO generation amount (NO) was calculated using the following formula (1): production %),in A control The absorbance corresponding to the cell model induced by LPS only.

[0138] NO production (%)= ×100% (1) The NO generation data of the snow lotus extracts obtained in Examples 1 and 3 and the corresponding reconstituted solutions, the snow lotus extracts obtained in Comparative Example 1 and the positive control group can be found in Figure 5 Among them, "dexamethasone" identifies the result data corresponding to the 10µg dexamethasone product, "+" identifies the result data corresponding to the cell model induced by only LPS, "-" identifies the result data corresponding to the cells in the natural state without LPS induction, "Example 1 Snow Lotus Extract" identifies the result data corresponding to the Snow Lotus Extract prepared in Example 1, "Example 1 Reconstituted Solution" identifies the result data corresponding to the reconstituted solution prepared in Example 1, "Example 3 Snow Lotus Extract" identifies the result data corresponding to the Snow Lotus Extract prepared in Example 3, "Example 3 Reconstituted Solution" identifies the result data corresponding to the reconstituted solution prepared in Example 3, and "Comparative Example 1" identifies the result data corresponding to the Snow Lotus Extract prepared in Comparative Example 1.

[0139] Depend on Figure 5 It can be seen that the NO generation inhibition activity experiment was carried out on the snow lotus extracts obtained in Examples 1 and 3, and the reconstituted solutions in Examples 1 and 3 without silica gel treatment, indicating that the snow lotus extracts obtained in Examples 1 and 3 and the corresponding reconstituted solutions have good anti-inflammatory activity.

[0140] Compared with the reconstituted solutions in Examples 1 and 3 without silica gel treatment, the anti-inflammatory activity of the snow lotus extracts obtained in Examples 1 and 3 changed very little, and the antioxidant activity effect increased slightly, which is consistent with the aforementioned changes in the (relative) content of total polyphenols, total flavonoids and corresponding target components in the products of Examples 1 and 3.

[0141] Moreover, at a concentration of 0.1%-0.4%, the snow lotus extract inhibited the generation of NO in a concentration gradient, showing strong anti-inflammatory activity. In addition, the antioxidant activity of the snow lotus extract (reconstituted solution) obtained in Examples 1 and 3 was better than that in Comparative Example 1, further indicating that the snow lotus extract prepared by the method of the present invention has a higher content of the target component.

[0142] Experimental Example 6 Evaluation of whitening activity The whitening activity was evaluated by melanin production inhibition activity detection. The specific test method is as follows: The prepared snow lotus extract / redissolved solution stock solution was used as a diluent with cell culture medium to prepare three material solutions of 0.1%, 0.2% and 0.4% respectively. The cells were mouse melanoma B16F10 cells. On the first day, the density of the cells was 2×10^4 cell / well in 24well. After 18 hours, the old culture medium solution was removed, and 1 mL of DMEM cell culture medium containing 1nM neuropeptide α-melanocyte stimulating hormone (α-MSH) was added to each well. Then, the prepared product solutions were added respectively. After culturing in the incubator for 24 hours, the supernatant was discarded, and then 1 mL of phosphate buffer solution (PBS) was added to wash the adherent cells. Then, the PBS solution was removed, and 200 μL of 1N NaOH solution was added to each well. The cells were dissolved at 80°C for about 30 minutes. After the end, 100 μL was taken and added to 96well. The absorbance was measured at a wavelength of 405nm using an enzyme marker to determine the amount of intracellular melanin secretion, where A control The absorbance corresponding to the cell model induced by α-MSH only.

[0143] Melanin production (%)= ×100% (2) The melanin secretion data of the snow lotus extracts obtained in Examples 1 and 3 and the corresponding reconstituted solutions, the snow lotus extracts obtained in Comparative Example 1 and the positive control group can be found in Figure 6Among them, "Kojic acid" identifies the result data corresponding to the application of 300Um kojic acid product, "+" identifies the result data corresponding to the application of only α-MSH-induced cell model, "-" identifies the result data corresponding to the application of cells in a natural state without α-MSH induction, "Example 1 Snow Lotus Extract" identifies the result data corresponding to the Snow Lotus Extract prepared in Example 1, "Example 1 Reconstituted Solution" identifies the result data corresponding to the reconstituted solution prepared in Example 1, "Example 3 Snow Lotus Extract" identifies the result data corresponding to the Snow Lotus Extract prepared in Example 3, "Example 3 Reconstituted Solution" identifies the result data corresponding to the reconstituted solution prepared in Example 3, and "Comparative Example 1" identifies the result data corresponding to the Snow Lotus Extract prepared in Comparative Example 1.

[0144] Depend on Figure 6 It can be seen that the melanin production inhibitory activity experiment was carried out on the snow lotus extracts obtained in Examples 1 and 3, and the reconstituted solutions in Examples 1 and 3 without silica gel treatment, indicating that the snow lotus extracts obtained in Examples 1 and 3 and the corresponding reconstituted solutions have good melanin production inhibitory activity.

[0145] Compared with the reconstituted solutions in Examples 1 and 3 without silica gel treatment, the melanin production inhibitory activity of the snow lotus extracts obtained in Examples 1 and 3 changed very little, and the melanin production inhibitory activity effect increased slightly, which is also consistent with the aforementioned changes in the (relative) content of total polyphenols, total flavonoids and corresponding target components in the products of Examples 1 and 3.

[0146] Moreover, at a concentration of 0.1%-0.4%, the snow lotus extract inhibited the production of melanin in a concentration gradient, and showed a stronger efficacy than the positive control kojic acid, showing a stronger whitening activity. In addition, the whitening activity of the snow lotus extract (reconstituted solution) obtained in Examples 1 and 3 was better than that of Comparative Example 1, further indicating that the snow lotus extract prepared by the method of the present invention has a higher content of the target component.

[0147] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a snow lotus extract, characterized in that: include: The alcohol extract of snow lotus was reconstituted and purified by polyol and adsorbent. The re-dissolution and purification process comprises: the alcohol extract of the snow lotus is re-dissolved in a polyol, mixed evenly with the adsorbent, and filtered; The adsorbent includes silica gel or macroporous adsorption resin; the amount of the adsorbent is 0.05%-5% of the total mass of the polyol and the alcohol extract of the snow lotus.

2. The preparation method according to claim 1, characterized in that The temperature of the purification treatment is 20-85°C; the time of the purification treatment is 5-90 minutes.

3. The preparation method according to claim 1, characterized in that: The polyol includes at least one of 1,3-butanediol and dipropylene glycol.

4. The preparation method according to claim 1, characterized in that: The mass fraction of the polyol is ≥50%.

5. The preparation method according to claim 1, characterized in that: The reconstitution temperature is 20-85°C.

6. The preparation method according to claim 1, characterized in that: The preparation method of the ethanol extract of snow lotus comprises: Snow lotus is extracted with ethanol, and the mass volume ratio of snow lotus to ethanol is 1:10-1:40; Extract at 45-85°C for 1-4h, filter; The mass fraction of the ethanol is ≥70%.

7. The preparation method according to claim 6, characterized in that: The method for preparing the snow lotus ethanol extract further comprises: removing ethanol from the solution obtained after filtering by using reduced pressure concentration treatment; The temperature of the reduced pressure concentration treatment is 38-55°C; the temperature of the reduced pressure concentration treatment is 42-48°C.

8. The preparation method according to claim 7, characterized in that: Before the snow lotus is mixed with ethanol, the snow lotus is sterilized by high-pressure steam; the pressure of the high-pressure steam sterilization is 103.4-205.8 kPa; the temperature of the high-pressure steam sterilization is 120-132°C.

9. A snow lotus extract, prepared according to the preparation method according to any one of claims 1-8.

10. Use of a snow lotus extract in preparing cosmetics, wherein the snow lotus extract comprises the snow lotus extract prepared by the preparation method according to any one of claims 1 to 8 or the snow lotus extract according to claim 9.

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