Bacteriostatic and deodorant composite preparation for pets and preparation method thereof

Abstract

The application provides a bacteriostatic and deodorizing composite preparation for pets and application thereof, which comprises 30-40% of enzyme preparation, 20-30% of plant extract, 20-30% of probiotic powder, and 0-10% of inorganic salt and filling aid. The application can efficiently remove ammonia and effectively reduce the ammonia gas yield in excrement; can efficiently inhibit E. coli, Salmonella, MRSA, C. perfringens and the like; can reduce fecal odor and effectively reduce fecal odor substances in pet excrement, such as indole, cadaverine, spermidine, putrescine and skatole and the like; is safe and harmless, avoids the negative influence of chemical synthetic substances on pet health, prevents clumping, and maximally reduces the influence on cat litter clumping, thereby guaranteeing the use effect.

Description

Technical Field

[0001] This invention relates to the field of pet product application technology, specifically to a compound preparation for pet antibacterial and deodorizing purposes and its preparation method. Background Technology

[0002] As pets play an increasingly important role in human life, their health issues are attracting growing attention. Pet feces, as a direct indicator of health, reflect digestive status. Excessive ammonia production indicates incomplete protein digestion, damaging intestinal epithelial cells, metabolism, and gut microbiota, disrupting acid-base balance, reducing digestive enzyme activity, and consequently affecting nutrient absorption. This also contributes to pet health problems and leads to significant ammonia emissions. Sulfides (such as hydrogen sulfide), ammonia, and aliphatic compounds (such as butyric acid and skatole) produced in feces and urine not only pollute the environment but also negatively impact the physiological functions of humans and animals. They stimulate the olfactory nerves, affect the respiratory center, impair respiratory function, and are highly toxic. With the continuous expansion of pet ownership, fecal pollution and odor problems are becoming increasingly serious. Statistics show that the number of pets in China has exceeded 100 million, generating a huge amount of feces annually.

[0003] The current pet feces deodorizer market largely relies on single functional substances such as yucca extract or inorganic salt systems like sodium bicarbonate, lacking consideration for digestion and resulting in poor deodorizing effects. Furthermore, issues like cat litter clumping limit widespread application, and these deodorizers offer limited features. Household users have a stronger demand for pet feces deodorization, but the current market offers limited product variety, primarily chemical deodorizers, with biological and physical deodorizers still in their early stages of development. As the demand for pet feces treatment increases, deodorizer technology needs innovation, especially in pet feces treatment, requiring a wider variety of products that are environmentally friendly and reduce ammonia emissions and pet feces odor. Summary of the Invention

[0004] To effectively target fecal odor by adding deodorizing agents, this invention addresses the technical problem of poor deodorization in current cat litter by synergistically combining bio-enzymes, probiotics, plant extracts, and inorganic salts. This reduces the abundance of odor-causing substances and pathogens in pet feces, lowers the risk of urinary tract infections in pets, and minimizes the impact of cat litter clumping on the deodorizing composition. This invention provides a pet antibacterial and deodorizing compound preparation and its preparation method.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a compound preparation capable of deodorizing and inhibiting bacteria, comprising 30%-40% enzyme preparation, 20%-30% plant extract, 20%-30% probiotic powder, and 0-10% inorganic salt and filler.

[0007] Preferably, the enzyme preparation is obtained by mixing laccase, lipase, glucose oxidase, peroxidase, pectinase, and protease in a mass ratio of 5:5:1:1:3:5.

[0008] Preferably, the protease in the enzyme preparation is obtained by mixing acidic protease, neutral protease and alkaline protease in a mass ratio of 5:3:2.

[0009] Preferably, the probiotic powder is obtained by mixing Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis in a mass ratio of 1:3:5.

[0010] Preferably, the plant extract is obtained by mixing dandelion extract, honeysuckle extract, and scutellaria baicalensis extract in a mass ratio of 1:1:1.

[0011] Preferably, the inorganic salt and filler are obtained by mixing zinc ricinoleate, sodium bicarbonate and montmorillonite in a mass ratio of 1:2:1.

[0012] A second aspect of the present invention provides a method for preparing a deodorizing and antibacterial compound formulation, wherein the preparation method comprises:

[0013] (1) Weigh out the enzyme preparation, probiotic powder, plant extract and inorganic salt adjuvant by weight, mix them and add them to the micro-particle granulator;

[0014] (2) After mixing corn starch and distilled water, heat to obtain corn starch solution, then spray corn starch into micro-granulator for granulation, and dry at low temperature to remove moisture to obtain compound preparation.

[0015] Preferably, the corn starch and distilled water are mixed at a material-to-liquid ratio of 1:(5~10), and the low-temperature drying temperature is 0~40℃.

[0016] A third aspect of the present invention provides the application of a deodorizing and antibacterial compound formulation. Application scenarios include, but are not limited to, pet feces treatment, cat litter, kennels, carpets, external use, and the removal of volatile odors from deodorizing substances.

[0017] The beneficial effects of this invention are:

[0018] This invention presents a compound antibacterial and deodorizing agent formulated with enzymes, probiotic powder, plant extracts, inorganic salts, and adjuvants. This compound works synergistically to effectively reduce ammonia production in feces and odor-causing substances in pet excrement, such as indole, cadaverine, spermidine, putrescine, and skatole. The enzymes and probiotics specifically decompose organic matter in pet excrement, particularly substances that cause odor. The enzymes' degradation of carbohydrates simultaneously provides more easily absorbed nutrients for the probiotics, promoting their recovery and further decomposing odor-causing substances in pet excrement.

[0019] Furthermore, the antibacterial and deodorizing compound preparation of the present invention can effectively inhibit Escherichia coli, Salmonella, methicillin-resistant Staphylococcus aureus (MRSA), Clostridium perfringens, etc. The enzyme preparation combination assists in the recovery and proliferation of probiotics, effectively squeezing the breeding space of harmful bacteria.

[0020] Furthermore, the deodorizing and antibacterial compound of the present invention, through optimized formulation and preparation process, can minimize the impact on cat litter clumping, thereby ensuring its effectiveness. The present invention employs a method of encapsulating the deodorizing and antibacterial compound with cornstarch. When finally applied, it is sprayed onto pet excrement, cat litter, kennels, and carpets. The starch encapsulation helps the probiotics to revive and break down, thus initiating the decomposition of excrement and minimizing clumping of excrement with cat litter and other products. Moreover, the deodorizing and antibacterial compound of the present invention does not cause adhesion to cat litter and other products, avoiding problems such as skin allergies and respiratory discomfort in pets. Attached Figure Description

[0021] Figure 1 It is a static ammonia production device.

[0022] Figure 2 This is the effect of Example 1 on fecal ammonia production. Detailed Implementation

[0023] The present invention will be further described below with reference to the embodiments.

[0024] The test materials used in the embodiments of this invention are all conventional test materials in the art and can be purchased through commercial channels.

[0025] Note: The laccase in this invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 10,000 U.

[0026] The lipase in this invention was produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 100,000 U.

[0027] The glucose oxidase in this invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 10,000 U.

[0028] The peroxidase in this invention was produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 10,000 U.

[0029] The pectinase in this invention was produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 300,000 U.

[0030] The protease in this invention was produced by Guangdong Yiduoli Biotechnology Co., Ltd., with an enzyme activity of 200,000 U.

[0031] The probiotic powder contains ≥1.0×10⁻⁶ Bacillus subtilis. 9 CFU / g, yeast content ≥1.0×10 7 CFU / g, Enterococcus faecalis content ≥1.0×10 8 CFU / g.

[0032] Dandelion extract, honeysuckle extract, scutellaria baicalensis extract, etc., were all purchased from Changsha Shiwei Biotechnology Co., Ltd.

[0033] Zinc ricinoleate, sodium bicarbonate, bentonite, etc., were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0034] The cat litter was provided by Hangzhou Gaoyejia. Example

[0035] Three different proteases were used: acidic protease, neutral protease, and alkaline protease. Each enzyme was added at a level of 50 units per gram (u / g) when used alone, and the degradation of plant-based raw protein, such as soybean meal, was performed under their respective optimal pH conditions. See Table 1 for details.

[0036] Table 1

[0037] Enzymes Add levels (u / g) Enzymatic hydrolysis environment Increase in soluble protein content (%) acidic protease 50 pH 3.0 75.77 neutral protease 50 pH 6.5 110.18 alkaline protease 50 pH 7.5 70.28

[0038] When used alone, neutral protease showed the highest increase in soluble protein content at pH 6.5, reaching 110.18%.

[0039] Subsequently, these three proteases were combined in different proportions to form four different experimental groups. The effects of different combinations on the increase rate of soluble protein content were evaluated under pH 6.5 conditions, as shown in Table 2.

[0040] Table 2

[0041] Grouping (proportion) acidic protease neutral protease alkaline protease Increase in soluble protein content (%) Group 1 5 3 2 154.81 Group 2 4 3 3 137.25 Group 3 2 4 4 107.20 Group 4 2 5 3 104.42

[0042] When used in combination, the first group (acidic protease: 5, neutral protease: 3, alkaline protease: 2) showed the highest increase in soluble protein content, at 154.81%, followed by the second group (acidic protease: 4, neutral protease: 3, alkaline protease: 3), with an increase in soluble protein content of 137.25%.

[0043] It can be seen that neutral protease performed best in the individual experiments. However, when acidic protease and alkaline protease were added without changing the amount of neutral protease, the increase in protein degradation content in the first and second groups indicates that the synergistic effect of different types of protease can significantly improve protein degradation efficiency. This is of great significance for improving the nutritional value and application range of raw materials. Example

[0044] Furthermore, based on Example 1, six enzymes—laccase, lipase, glucose oxidase, peroxidase, pectinase, and protease (acidic protease: 5, neutral protease: 3, alkaline protease: 2)—were used to treat pet excrement. By varying the proportions of these enzymes, five different experimental combinations were created to evaluate their effects on ammonia production. See the experimental setup diagram below. Figure 1 .

[0045] Ammonia is produced by the fermentation of pet excrement. The change in ammonia content after adding a deodorizing and antibacterial compound preparation was measured to evaluate the deodorizing effect of the product. The detection method was based on GB / T 14668-1993 Determination of ammonia in air quality, Nessler's reagent colorimetric method.

[0046] Take fresh cat feces and place equal amounts into feces bottles (apparatus as follows). Figure 1 As shown in Table 3, enzyme systems prepared in groups one through five were added to each feces bottle at 3% of the cat litter content. The feces bottles were placed in a room at 28°C, and the ammonia production of each group was measured after 3 days using Nessler's reagent colorimetric method. The results are shown in Table 3.

[0047] Enzymatic hydrolysis was carried out under controlled conditions, and the effectiveness of the enzyme combination was evaluated by measuring the ammonia production in the reaction system.

[0048] Table 3

[0049] Grouping (proportion) Laccase Lipase glucose oxidase peroxidase pectinase protease Ammonia production (μg) Group 1 5 5 1 1 3 5 32.82213 Group 2 4 4 2 2 3 5 38.4933 Group 3 3 3 3 3 3.5 4.5 47.59035 Group 4 4 3 2 2 5 4 33.03507 Group 5 5 4 1 1 4 5 34.85448

[0050] The first enzyme combination (laccase: 5, lipase: 5, glucose oxidase: 1, peroxidase: 1, pectinase: 3, protease: 5) produced the least amount of ammonia, 32.82213 μg.

[0051] Experimental results show that different enzyme combinations have a significant impact on the amount of ammonia produced, and some combinations can more effectively inhibit the formation of odorous substances such as ammonia. Based on Examples 1 and 2 above, the optimal enzyme ratio combination (Group 1) was selected.

[0052] Example 3 (Full Combination Example)

[0053] A 10 kg deodorizing and antibacterial compound preparation was prepared, comprising: laccase: 0.25 kg, lipase: 0.25 kg, glucose oxidase: 0.25 kg, peroxidase: 0.75 kg, pectinase: 0.75 kg, Bacillus subtilis powder: approximately 0.11 kg, yeast: approximately 0.33 kg, Enterococcus faecalis: approximately 0.56 kg, dandelion extract: approximately 0.33 kg, honeysuckle extract: approximately 0.33 kg, Scutellaria baicalensis extract: approximately 0.33 kg, zinc ricinoleate: approximately 0.33 kg, sodium bicarbonate: approximately 0.67 kg, with the remainder filled with montmorillonite. After mixing, the mixture was added to a microgranulator at a speed of 25 r / min, then sprayed with a corn starch solution (2 kg corn starch dissolved in 10 kg water and heated to 45°C) for granulation. The mixture was then dried at low temperature to remove moisture, yielding the compound preparation.

[0054] Example 4 (Example without enzyme preparation)

[0055] A 10 kg deodorizing and antibacterial compound preparation was prepared, comprising approximately 0.11 kg of Bacillus subtilis powder, approximately 0.33 kg of yeast, approximately 0.56 kg of Enterococcus faecalis, approximately 0.33 kg of dandelion extract, approximately 0.33 kg of honeysuckle extract, approximately 0.33 kg of Scutellaria baicalensis extract, approximately 0.33 kg of zinc ricinoleate, and approximately 0.67 kg of sodium bicarbonate. The remainder was filled with montmorillonite and rice bran. After mixing, the mixture was added to a microgranulator at a speed of 25 r / min, and then sprayed with a corn starch solution (2 kg of corn starch dissolved in 10 kg of water and heated to 45°C) for granulation. The mixture was then dried at low temperature to remove moisture, yielding the compound preparation.

[0056] Example 5 (Example without probiotics)

[0057] A 10 kg deodorizing and antibacterial compound preparation was prepared, comprising: laccase: 0.25 kg, lipase: 0.25 kg, glucose oxidase: 0.25 kg, peroxidase: 0.75 kg, pectinase: 0.75 kg, dandelion extract: approximately 0.33 kg, honeysuckle extract: approximately 0.33 kg, scutellaria baicalensis extract: approximately 0.33 kg, zinc ricinoleate: approximately 0.33 kg, sodium bicarbonate: approximately 0.67 kg, with the remainder filled with montmorillonite and rice bran. After mixing, the mixture was added to a microgranulator at a speed of 25 r / min, then sprayed with a corn starch solution (2 kg corn starch dissolved in 10 kg water and heated to 45°C) for granulation. The mixture was then dried at low temperature to remove moisture, yielding the compound preparation.

[0058] Example 6 (Example without plant extracts)

[0059] A 10 kg deodorizing and antibacterial compound preparation was prepared, comprising: 0.25 kg laccase, 0.25 kg lipase, 0.25 kg glucose oxidase, 0.75 kg peroxidase, 0.75 kg pectinase, approximately 0.11 kg Bacillus subtilis powder, approximately 0.33 kg yeast, approximately 0.56 kg Enterococcus faecalis, approximately 0.33 kg zinc ricinoleate, and approximately 0.67 kg sodium bicarbonate. The remainder was filled with montmorillonite and rice bran. After mixing, the mixture was added to a microgranulator at a speed of 25 r / min, and then sprayed with a corn starch solution (2 kg corn starch dissolved in 10 kg water and heated to 45 °C) for granulation. The mixture was then dried at low temperature to remove moisture, yielding the compound preparation.

[0060] Example 7 (Example without inorganic salts and fillers)

[0061] A 10 kg deodorizing and antibacterial compound preparation was prepared, comprising: laccase: 0.25 kg, lipase: 0.25 kg, glucose oxidase: 0.25 kg, peroxidase: 0.75 kg, pectinase: 0.75 kg, dandelion extract: approximately 0.33 kg, honeysuckle extract: approximately 0.33 kg, scutellaria baicalensis extract: approximately 0.33 kg, Bacillus subtilis powder: approximately 0.11 kg, yeast: approximately 0.33 kg, Enterococcus faecalis: approximately 0.56 kg, with the remainder filled with rice bran. After mixing, the mixture was added to a microgranulator at a speed of 25 r / min, then sprayed with a corn starch solution (2 kg corn starch dissolved in 10 kg water, heated to 45°C) for granulation. The mixture was then dried at low temperature to remove moisture, yielding the compound preparation. Example

[0062] 1. Deodorization test

[0063] Ammonia is produced by the fermentation of cat feces. The change in ammonia content after adding a deodorizing and antibacterial compound preparation was measured to evaluate the deodorizing effect of the product. The detection method was based on GB / T 14668-1993 Determination of ammonia in air quality, Nessler's reagent colorimetric method.

[0064] Take fresh cat feces and place equal amounts into feces bottles (apparatus as follows). Figure 1 As shown in the figure, 3% of the cat litter content of each feces bottle was added to the deodorizing and antibacterial compound preparation prepared in Examples 2-5, and the control group was not added. The feces bottles were placed in a room at room temperature of 28°C, and the ammonia production of each group was measured on the first day, the second day, the third day, the fifth day and the seventh day. The determination was performed by Nessler's reagent colorimetric method, and the results are shown in Table 4.

[0065] Table 4. Deodorization effect of each embodiment

[0066] Group / Ammonia μg Day 1 Day 3 Day 5 Day 7 control group 12.42 26.36 47.59 53.66 Example 3 2.71 19.09 20.30 29.72 Example 4 10.60 31.82 38.49 50.02 Example 5 10.60 27.26 31.44 31.97 Example 6 8.78 30.00 53.66 42.74 Example 7 6.35 19.69 26.97 32.43

[0067] A comparison of the data from the control group and Example 3 shows that the antibacterial and deodorizing compound preparation of the present invention is significantly more effective in removing ammonia than other examples. Figure 2 The ammonia production of the control group and Example 3 on days 1, 3, 5, and 7 is shown, as well as the percentage of ammonia production inhibition by Example 3. Figure 2 The results showed that on the first day, Example 3 exhibited the highest inhibition of ammonia production, reaching 78.2%. This was followed by the fifth day, with an inhibition rate of 47.27%, indicating that the enzyme preparation in the antibacterial and deodorizing compound formulation was highly effective in deodorization.

[0068] On the other hand, when comparing Examples 3 and Examples 4-7, it can be found that the deodorizing effect is reduced to varying degrees when one of the components of the antibacterial and deodorizing compound preparation of the present invention is missing. In particular, in Example 4, where the enzyme preparation is missing, the ammonia production is close to that of the control group on the seventh day.

[0069] Meanwhile, it should be noted that the ammonia production data on the seventh day, lacking any one of the components of the antibacterial and deodorizing compound preparation of the present invention, could not achieve the effect of Example 3.

[0070] 2. Antibacterial test

[0071] An aqueous solution was prepared by adding 3% of the samples from Examples 3-7. Subsequently, antibacterial experiments were conducted using Escherichia coli, Salmonella, Staphylococcus aureus, and Clostridium perfringens as indicator bacteria. The antibacterial results are shown in Table 5.

[0072] Table 5. Results of antibacterial and deodorizing compound preparation's antibacterial test.

[0073] Antibacterial zone diameter / mm E. coli salmonella MRSA Clostridium perfringens Example 3 10 11 10 12 Example 4 7 8 6 8 Example 5 5 6 7 6 Example 6 6 5 6 7 Example 7 9 10 8 10

[0074] As shown in Table 5, the antibacterial and deodorizing compound preparation provided in Example 3 can effectively inhibit Escherichia coli, Salmonella, MRSA and Clostridium perfringens. Overall, it has good antibacterial properties, while inorganic salts and filler additives have no obvious antibacterial effect.

[0075] 3. Reduce fecal odor substances

[0076] The remaining feces from the control group in Example 8 and Example 3 were tested for fecal odor substances, and the results are shown in Table 6.

[0077] Table 6. Effects of barley odorant and glucose oxidase on canine odor compounds.

[0078] Project ng / g Indole Cadaniamine spermidine ammonia skatole control group 15193.67 59138.75 353.33 38547.45 254.77 Example 3 10173.88 47654.66 297.65 29845.62 120.85

[0079] Note: All the above indicators are in ng / g.

[0080] As shown in Table 6, compared with the control group, the fecal odor substances in the feces treated with the antibacterial and deodorizing compound preparation were significantly reduced, indicating that the antibacterial and deodorizing effect was superior.

[0081] The above experimental results show that the combination of several substances has a significant synergistic effect, indicating that the combination has a synergistic effect rather than a simple additive effect. The enzyme preparation, plant extract, probiotics, and inorganic salts provided by this invention can effectively enhance the function of the compound product, with a clear amplification effect. In summary, the antibacterial and deodorizing compound preparation provided by this invention can efficiently remove ammonia, effectively reducing the ammonia production in feces; it is highly effective in inhibiting bacteria such as Escherichia coli, Salmonella, MRSA, and Clostridium perfringens; it reduces fecal odor, effectively reducing odorous substances in pet feces, such as indole, cadaverine, spermidine, putrescine, and skatole; it is safe and harmless, avoiding the negative impact of chemically synthesized substances on pet health; and it prevents clumping, minimizing the impact on cat litter clumping, thereby ensuring its effectiveness.

[0082] The embodiments described above can be further combined or replaced, and these embodiments are merely descriptions of preferred embodiments of the present invention, not limitations on the concept and scope of the present invention. Various changes and improvements made to the technical solutions of the present invention by those skilled in the art without departing from the inventive concept are all within the protection scope of the present invention. The protection scope of the present invention is given by the appended claims and any equivalents.

Claims

1. A compound preparation for pets that inhibits bacteria and deodorizes, characterized in that, Components including the following mass percentages: Enzyme preparations 30%-40%, plant extracts 20%-30%, probiotic powder 20%-30%, inorganic salts and fillers 0-10%; The enzyme preparation is obtained by mixing laccase, lipase, glucose oxidase, peroxidase, pectinase, and protease in a mass ratio of 5:5:1:1:3:

5. The protease in the enzyme preparation is obtained by mixing acidic protease, neutral protease and alkaline protease in a mass ratio of 5:3:2; The probiotic powder is obtained by mixing Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis in a mass ratio of 1:3:

5. The plant extract is obtained by mixing dandelion extract, honeysuckle extract, and scutellaria baicalensis extract in a mass ratio of 1:1:

1. The inorganic salt and filler were obtained by mixing zinc ricinoleate, sodium bicarbonate and montmorillonite in a mass ratio of 1:2:

1. The preparation method of the compound formulation is as follows: (1) Weigh out enzyme preparations, probiotic powder, plant extracts, inorganic salts and filler, mix them and add them to the micro-particle granulator; (2) After mixing corn starch and distilled water, heat to obtain corn starch solution, then spray the corn starch solution into a micro-granulator for granulation, and dry at low temperature to remove moisture to obtain a compound preparation; the corn starch and distilled water are mixed at a material-to-liquid ratio of 1: (5~10), and the low temperature drying temperature is 0~40℃.

Images