A kind of lactating sow compound feed and preparation method thereof

Abstract

The invention discloses a lactating sow compound feed and a preparation method thereof, and relates to the technical field of feed processing; the lactating sow compound feed consists of peony seed oil nanoemulsion, an amino acid chelated trace element composite bag and corn flour; the peony seed oil nanoemulsion consists of peony seed oil, modified attapulgite, soy protein isolate, trehalose and chitosan hydrochloride; the amino acid chelated trace element composite bag consists of amino acid chelated iron, Chinese herbal medicine powder and bacillus subtilis; the nanoemulsion is used as a carrier material to coat the peony seed oil as an effective ingredient, and the amino acid chelated trace element composite bag is attached to the surface of the nanoemulsion, so as to prepare the lactating sow compound feed, which not only provides comprehensive nutritional support, supplements minerals and vitamin substances, ensures nutritional balance, but also improves the bioavailability of nutrients in the feed, and effectively promotes the lactation performance of the sow.

Description

Technical Field

[0001] The invention belongs to the technical field of feed processing, and specifically relates to a lactating sow compound feed and a preparation method thereof. Background Art

[0002] Pig colostrum is not only rich in immunoglobulins, which can improve immunity and meet the nutritional and immune needs of piglets after birth, but also rich in magnesium ions, which can promote the excretion of meconium in piglets. However, not every sow, especially primiparous sows, can lactate in time, and insufficient secretion is also the most common condition. Therefore, the application of lactation feed can effectively solve the problem of low lactation ability of sows.

[0003] The main functions of lactation feed are to promote mammary gland development, increase milk production, improve milk quality, regulate the balance of intestinal flora, increase appetite, reduce constipation, enhance animal vitality, reduce culling rate, improve animal body condition, shorten estrus cycle, extend service life, reduce weak fetuses and stillbirths, etc., and significantly increase the number of healthy piglets. Lactation feed has a positive effect on the lactation performance of mammals through its specific ingredients and mechanism of action.

[0004] The existing technology currently has the following problems:

[0005] Although lactation feed contains high protein, it lacks minerals and vitamins such as calcium and iron, leading to nutritional imbalance. At the same time, the bioavailability of lactation feed is low, which further limits the feeding effect of lactation feed and is not conducive to improving the lactation and production performance of sows. Summary of the invention

[0006] In view of the above situation, in order to overcome the defects of the prior art, the present invention proposes a lactating sow compound feed, comprising the following components in parts by weight: 30-50 parts of peony seed oil nanoemulsion, 8-10 parts of amino acid chelated trace element composite package, and 80-90 parts of corn flour.

[0007] The peony seed oil nanoemulsion comprises the following components in parts by weight: 8-12 parts of peony seed oil, 10-20 parts of modified attapulgite, 10-20 parts of isolated soy protein, 20-30 parts of trehalose and 10-15 parts of chitosan hydrochloride.

[0008] The amino acid chelated trace element composite package comprises the following components in parts by weight: 10-20 parts of amino acid chelated iron, 10-20 parts of Chinese herbal medicine powder, and 10-15 parts of Bacillus subtilis.

[0009] The raw materials of the Chinese herbal medicine powder include the following components in parts by weight: 3-5 parts of astragalus, 3-5 parts of dandelion, and 3-5 parts of honeysuckle.

[0010] The preparation method of the peony seed oil nanoemulsion specifically comprises the following steps:

[0011] (1) Disperse 20 g of attapulgite in 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add monohydrated citric acid to adjust the pH to 3.0, stir magnetically at room temperature for 8-10 h, centrifuge at a centrifugal speed of 5000-7000 rpm, and centrifuge for 5-10 min. Wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush. Acidification can change the charge and adsorption activity of attapulgite, help depolymerize mineral aggregates, and make it more dispersible. In addition, attapulgite also contains major and trace elements necessary for livestock and poultry, such as copper, zinc, manganese, iron, cobalt, molybdenum and selenium, which are essential for the growth and development of animals, can improve the body's immune and antioxidant functions, and improve the nutrient utilization rate of feed and animal production performance, thereby obtaining modified attapulgite;

[0012] (2) Dissolve 0.1-0.2 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.2-0.3 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution, stir magnetically at room temperature for 1-2 h, and hydrate in a refrigerator at 4°C for 8-12 h. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 600-800 W and the ultrasonic time is 10-15 min. The ultrasonic treatment also improves the foaming performance of the soy protein isolate, which is beneficial to emulsification, to obtain an aqueous phase solution;

[0013] (3) dropping peony seed oil into the aqueous phase solution described in step (2), wherein the modified attapulgite is used as a stabilizing carrier, the soy protein isolate is used as an emulsifier, and trehalose promotes the soy protein isolate to stabilize more oil-water interfaces, thereby reducing the flocculation and agglomeration degree of the nanoemulsion during the homogenization process, ensuring the uniformity and stability of the particle size, and dispersing at high speed to obtain a coarse emulsion at a speed of 10000-12000 rpm for 3-5 min. The coarse emulsion is then mixed with a chitosan hydrochloride aqueous solution having a mass fraction of 5-10%, the pH value is adjusted to 5.0-5.5, and the mixture is stirred at 50-55° C. for 2-3 h. The mixture is then homogenized at high pressure at 20-30 MPa for 3-5 min to form a spherical granular coating. The chitosan hydrochloride as a wall material further enhances the stability and utilization of the peony seed oil, has mucosal adhesion and positive charge characteristics, and can promote the intestinal absorption of nutrients and the proliferation and differentiation of mammary epithelial cells, thereby obtaining a peony seed oil nanoemulsion;

[0014] Preferably, in step (2), the amount of soy protein isolate added is 0.1-0.2 g, and the soy protein isolate not only provides the required protein substance, supports the basic nutritional needs of animals during lactation, but also has the function of promoting lactation;

[0015] Preferably, in step (3), the amount of peony seed oil added is 3-5 mL. Peony seed oil has a special aromatic smell, which can stimulate the secretion of saliva and amylase in lactating sows, inhibit the number of harmful intestinal bacteria, increase the height of intestinal villi, and promote the digestion and absorption of nutrients, thereby promoting the production of nutrients such as milk protein, lactose, and milk solids in milk, thereby improving the milk production and milk quality of sows.

[0016] The present invention also provides a method for preparing a lactating sow compound feed, which specifically comprises the following steps:

[0017] S1. Firstly crush the astragalus, dandelion and honeysuckle, the mesh size is 60-80 mesh, and then ultrafinely grind, the mesh size is 200-300 mesh. Chinese herbal medicine contains a large number of functional active ingredients, which can improve the lactation performance and milk quality of sows, and provide some biologically active substances with anti-inflammatory, bactericidal and immunity-enhancing properties through breast milk, which is of great significance for maintaining the early healthy growth of piglets. The collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis are placed in a ball mill for grinding for 20-30 minutes, and the speed is controlled at 2000-4000rpm. The Chinese herbal medicine powder of the tonifying and replenishing qi type promotes the proliferation and metabolism of Bacillus subtilis and plays a prebiotic role. A mutually dispersed complex is formed between the three, which is conducive to maximizing the synergistic effect of each component, effectively enhancing the therapeutic effect, and obtaining an amino acid chelated trace element composite package;

[0018] S2, slowly adding the amino acid chelated trace element composite package described in step S1 to the peony seed oil nanoemulsion, magnetically stirring for 20-30 minutes, and then freeze-drying at a drying temperature of -30°C to -40°C for 8-10 hours. The amino acid chelated trace element composite package can be adsorbed on the surface structure of the peony seed oil nanoemulsion, further promoting the absorption of elements and nutrients, and then mixing evenly with corn flour, granulating, and obtaining a lactating sow compound feed;

[0019] Preferably, in step S1, the activity of Bacillus subtilis is 1.0-2.0×10 6 cfu / g. The presence of Bacillus subtilis can degrade the cell walls of Chinese herbal medicines, allowing the effective ingredients to precipitate in the digestive tract. It can also promote the absorption and utilization of amino acid chelated iron in the intestines, enhancing nutrition and lactation performance.

[0020] The beneficial effects achieved by the present invention are as follows:

[0021] The invention uses nanoemulsion as a carrier material, encapsulates the effective ingredient peony seed oil, and simultaneously attaches an amino acid chelated trace element composite package on the surface of the nanoemulsion to prepare a lactating sow compound feed, which not only provides comprehensive nutritional support, supplements minerals and vitamins, ensures nutritional balance, but also improves the bioavailability of nutrients in the feed, and effectively promotes the lactation performance of the sow; in the peony seed oil nanoemulsion, an aqueous phase solution formed by modified attapulgite, trehalose and soy protein isolate encapsulates the peony seed oil, and chitosan hydrochloride is used as a wall material to further enhance the stability of the peony seed oil, and the obtained nanoemulsion has a uniform and stable particle size, and chitosan hydrochloride is loaded with effective ingredients, which increases the residence time in the intestinal mucosa and is beneficial to the absorption and utilization of each component, wherein the peony seed oil can promote the generation of nutrients such as milk protein, lactose, and milk solids in milk, and improve the milk yield and milk quality of the sow, and the peony seed oil contains magnesium, potassium, iron, zinc, calcium and other trace elements. The amino acid chelated trace element composite package contains active ingredients such as iron and vitamin E, which makes up for the nutritional imbalance in traditional lactation feeds; in the amino acid chelated trace element composite package, a mutually dispersed block complex is formed, Bacillus subtilis is beneficial to the absorption and utilization of Chinese herbal medicine and amino acid chelated iron, and reduces toxic side effects. At the same time, Chinese herbal medicine promotes the proliferation and metabolism of Bacillus subtilis, and the three act synergistically, significantly enhancing the improvement effect on the lactation performance of sows; the amino acid chelated trace element composite package is adsorbed on the surface reticular structure of the peony seed oil nanoemulsion, which increases the absorption of each component in the composite package, and the composite combination of the composite package and the nanoemulsion is also helpful to improve the intestinal function of the sow, thereby helping to improve the lactation performance; the present invention uses peony seed oil nanoemulsion, amino acid chelated trace element composite package and corn flour to prepare a lactating sow compound feed, which supplements nutrients such as minerals and vitamins, ensures the nutritional balance of the feed, and also improves the absorption and utilization of nutrients in the feed, effectively improving the lactation performance of the sow. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a scanning electron microscope image of the peony seed oil nanoemulsion prepared in Example 1 of the present invention;

[0023] Figure 2 This is a scanning electron microscope image of the amino acid chelated trace element composite package prepared in Example 1 of the present invention;

[0024] Figure 3 The milk production results of Examples 1-4 and Comparative Examples 1-3 of the present invention are shown in FIG.

[0025] Figure 4 The figure is a result diagram of the nutritional components of milk of Examples 1-4 and Comparative Examples 1-3 of the present invention;

[0026] Figure 5 It is a graph showing the lactation performance results of Examples 1-4 of the present invention and Comparative Examples 1-3. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0028] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0029] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0030] The sources of the reagents used in the examples are as follows:

[0031] Peony seed oil CAS No: 9004-34-6, brand Heze Guyu Peony Biotechnology Co., Ltd., specification 280mL;

[0032] Attapulgite CAS No: 12174-11-7, brand Macklin, product number A921976-250mg;

[0033] Sodium hexametaphosphate CAS No: 10124-56-8, brand Innochem, product number A80460;

[0034] Citric acid monohydrate CAS No: 5949-29-1, brand Innochem, product number A63085;

[0035] Trehalose CAS No: 99-20-7, brand Innochem, product number A52221;

[0036] PBS buffer solution brand: Krypton, product number: CD433577-100mL;

[0037] Soy protein isolate CAS No: 9010-10-0, brand Macklin, item number S832685-50g;

[0038] Chitosan hydrochloride CAS No: 70694-72-3, brand Macklin, product number 850122-25g;

[0039] Amino acid chelated iron brand Yuanye, item number S20421-25g;

[0040] Bacillus subtilis CMCC (B) 63501 brand: Huankai Microorganisms, product number: 10014525.

[0041] Example 1

[0042] This embodiment provides a lactating sow compound feed, comprising the following components in parts by weight: 50 parts of peony seed oil nanoemulsion, 10 parts of amino acid chelated trace element composite package, and 90 parts of corn flour.

[0043] The peony seed oil nanoemulsion comprises the following components in parts by weight: 12 parts of peony seed oil, 20 parts of modified attapulgite, 20 parts of isolated soy protein, 30 parts of trehalose and 15 parts of chitosan hydrochloride.

[0044] The amino acid chelated trace element composite package comprises the following components in parts by weight: 20 parts of amino acid chelated iron, 20 parts of Chinese herbal medicine powder, and 15 parts of Bacillus subtilis.

[0045] The raw materials of the Chinese herbal medicine powder include the following components in parts by weight: 5 parts of astragalus, 5 parts of dandelion and 5 parts of honeysuckle.

[0046] The preparation method of peony seed oil nanoemulsion specifically comprises the following steps:

[0047] (1) Disperse 20 g of attapulgite in 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add monohydrated citric acid to adjust the pH to 3.0, stir magnetically at room temperature for 10 h, centrifuge at a speed of 7000 rpm, and centrifuge for 10 min. Wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush. Acidification can change the charge and adsorption activity of attapulgite, help depolymerize mineral aggregation, and make it more dispersible. In addition, attapulgite also contains major and trace elements necessary for livestock and poultry, such as copper, zinc, manganese, iron, cobalt, molybdenum and selenium, which are essential for the growth and development of animals, can improve the body's immune and antioxidant functions, and improve the nutrient utilization rate of feed and animal production performance, thereby obtaining modified attapulgite;

[0048] (2) Dissolve 0.2 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.3 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution. The amount of soy protein isolate added is 0.2 g. The soy protein isolate not only provides the required protein substance and supports the basic nutritional needs of animals during lactation, but also has the function of promoting lactation. Stir magnetically at room temperature for 2 hours, and then hydrate in a refrigerator at 4°C for 12 hours. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 800 W and the ultrasonic time is 15 minutes. The ultrasonic treatment also improves the foaming performance of the soy protein isolate, which is beneficial to emulsification, and an aqueous phase solution is obtained.

[0049] (3) Add peony seed oil dropwise into the aqueous phase solution described in step (2), wherein the amount of peony seed oil added is 5 mL. Peony seed oil has a special aromatic smell, which can stimulate the secretion of saliva and amylase in lactating sows, inhibit the number of harmful intestinal bacteria, increase the height of intestinal villi, and promote the digestion and absorption of nutrients, thereby promoting the production of nutrients such as milk protein, lactose, and milk solids in milk, thereby increasing the milk production and milk quality of sows. In the aqueous phase solution, modified attapulgite is used as a stabilizing carrier, soy protein isolate is used as an emulsifier, and trehalose promotes soy protein isolate to stabilize more oil-water interfaces, thereby reducing the nanoemulsion during the homogenization process. The flocculation and agglomeration degree was determined to ensure the uniformity and stability of the particle size. A crude emulsion was obtained by high-speed dispersion at a speed of 12000 rpm for 5 min. The crude emulsion was then mixed with a 10% by mass chitosan hydrochloride aqueous solution, the pH was adjusted to 5.5, and the mixture was stirred at 55 °C for 3 h. Then, high-pressure homogenization was performed at 30 MPa for 5 min to form a spherical granular coating. Chitosan hydrochloride as a wall material further enhanced the stability and utilization of peony seed oil, had mucosal adhesion and positive charge characteristics, and could promote the intestinal absorption of nutrients and the proliferation and differentiation of mammary epithelial cells to obtain peony seed oil nanoemulsion.

[0050] The present embodiment provides a method for preparing a lactating sow compound feed, which specifically comprises the following steps:

[0051] S1. Astragalus, dandelion and honeysuckle were first crushed with a sieve aperture of 80 mesh, and then ultrafinely crushed with a sieve aperture of 300 mesh. Chinese herbal medicines contain a large number of functional active ingredients, which can improve the lactation performance and milk quality of sows, and provide some biologically active substances with anti-inflammatory, bactericidal and immunity-enhancing effects through breast milk, which is of great significance for maintaining the early healthy growth of piglets. The collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis were placed in a ball mill for grinding for 30 minutes, and the speed was controlled at 4000rpm. The activity of Bacillus subtilis was 2.0×10 6cfu / g, the presence of Bacillus subtilis can degrade the cell wall in Chinese herbal medicine, so that the effective ingredients therein are precipitated in the digestive tract, and can also promote the absorption and utilization of amino acid chelated iron in the intestine, enhance nutrition and lactation performance, and the Chinese herbal medicine powder of tonifying the middle and replenishing qi promotes the proliferation and metabolism of Bacillus subtilis and plays a prebiotic role. The three form a mutually dispersed complex, which is conducive to maximizing the synergistic effect of each component, effectively enhancing the therapeutic effect, and obtaining an amino acid chelated trace element composite package;

[0052] S2. Slowly add the amino acid chelated trace element composite package described in step S1 into the peony seed oil nanoemulsion, stir magnetically for 30 minutes, and then freeze-dry at a drying temperature of -40°C for a drying time of 10 hours. The amino acid chelated trace element composite package can be adsorbed on the surface structure of the peony seed oil nanoemulsion, further promoting the absorption of elements and nutrients. Then, the composite package is evenly mixed with corn flour and granulated to obtain a lactating sow compound feed.

[0053] In this example, the prepared peony seed oil nanoemulsion and amino acid chelated trace element composite package were subjected to scanning electron microscopy to observe their microscopic morphology. Figure 1 This is a SEM image of the peony seed oil nanoemulsion prepared in Example 1 magnified 4000 times. Figure 2 This is a 200-fold magnified SEM image of the amino acid chelated trace element composite package prepared in Example 1. Figure 1 The surface of the peony seed oil nanoemulsion prepared in this embodiment presents a network porous structure, such as Figure 2 The amino acid chelated trace element composite package prepared in this embodiment is in the form of dispersed blocks.

[0054] Example 2

[0055] This embodiment provides a lactating sow compound feed, comprising the following components in parts by weight: 30 parts of peony seed oil nanoemulsion, 8 parts of amino acid chelated trace element composite package, and 80 parts of corn flour.

[0056] The peony seed oil nanoemulsion comprises the following components in parts by weight: 8 parts of peony seed oil, 10 parts of modified attapulgite, 10 parts of isolated soy protein, 20 parts of trehalose and 10 parts of chitosan hydrochloride.

[0057] The amino acid chelated trace element composite package comprises the following components in parts by weight: 10 parts of amino acid chelated iron, 10 parts of Chinese herbal medicine powder, and 10 parts of Bacillus subtilis.

[0058] The raw materials of the Chinese herbal medicine powder include the following components in parts by weight: 3 parts of astragalus, 3 parts of dandelion and 3 parts of honeysuckle.

[0059] The preparation method of peony seed oil nanoemulsion specifically comprises the following steps:

[0060] (1) Disperse 20 g of attapulgite in 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add monohydrated citric acid to adjust the pH to 3.0, stir magnetically at room temperature for 8 h, centrifuge at a speed of 5000 rpm for 5 min, wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush. Acidification can change the charge and adsorption activity of attapulgite, help depolymerize mineral aggregates, and make it more dispersible. In addition, attapulgite also contains major and trace elements necessary for livestock and poultry, such as copper, zinc, manganese, iron, cobalt, molybdenum and selenium, which are essential for the growth and development of animals, can improve the body's immune and antioxidant functions, and improve the nutrient utilization rate of feed and animal production performance, thereby obtaining modified attapulgite;

[0061] (2) Dissolve 0.1 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.2 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution. The amount of soy protein isolate added is 0.1 g. The soy protein isolate not only provides the required protein substance and supports the basic nutritional needs of animals during lactation, but also has the function of promoting lactation. Stir magnetically at room temperature for 1 hour, and then hydrate in a refrigerator at 4°C for 8 hours. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 600 W and the ultrasonic time is 10 min. The ultrasonic treatment also improves the foaming performance of the soy protein isolate, which is beneficial to emulsification, to obtain an aqueous phase solution.

[0062] (3) Peony seed oil is dripped into the aqueous phase solution described in step (2), and the amount of peony seed oil added is 3 mL. Peony seed oil has a special aromatic smell, which can stimulate the secretion of saliva and amylase in lactating sows, inhibit the number of harmful intestinal bacteria, increase the height of intestinal villi, and promote the digestion and absorption of nutrients, thereby promoting the production of nutrients such as milk protein, lactose, and milk solids in milk, thereby improving the milk production and milk quality of sows. In the aqueous phase solution, modified attapulgite is used as a stabilizing carrier, soy protein isolate is used as an emulsifier, and trehalose promotes soy protein isolate to stabilize more oil-water interfaces, thereby weakening the nanoemulsion during the homogenization process. The flocculation and agglomeration degree in the mixture ensured the uniformity and stability of the particle size. The crude emulsion was obtained by high-speed dispersion at a speed of 10,000 rpm for 3 min. The crude emulsion was then mixed with a 5% by mass chitosan hydrochloride aqueous solution, the pH was adjusted to 5.0, and the mixture was stirred at 50 ° C for 2 h. Then, the mixture was homogenized at high pressure at 20 MPa for 3 min to form a spherical granular coating. Chitosan hydrochloride as a wall material further enhanced the stability and utilization of peony seed oil, had mucosal adhesion and positive charge characteristics, and could promote the intestinal absorption of nutrients and the proliferation and differentiation of mammary epithelial cells to obtain peony seed oil nanoemulsion.

[0063] The present embodiment provides a method for preparing a lactating sow compound feed, which specifically comprises the following steps:

[0064] S1. Astragalus, dandelion and honeysuckle were first crushed with a sieve aperture of 60 mesh, and then ultrafinely crushed with a sieve aperture of 200 mesh. Chinese herbal medicines contain a large number of functional active ingredients, which can improve the lactation performance and milk quality of sows, and provide some biologically active substances with anti-inflammatory, bactericidal and immunity-enhancing effects through breast milk, which is of great significance for maintaining the early healthy growth of piglets. The collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis were placed in a ball mill for 20 minutes, and the speed was controlled at 2000rpm. The activity of Bacillus subtilis was 1.0×10 6 cfu / g, the presence of Bacillus subtilis can degrade the cell wall in Chinese herbal medicine, so that the effective ingredients therein are precipitated in the digestive tract, and can also promote the absorption and utilization of amino acid chelated iron in the intestine, enhance nutrition and lactation performance, and the Chinese herbal medicine powder of tonifying the middle and replenishing qi promotes the proliferation and metabolism of Bacillus subtilis and plays a prebiotic role. The three form a mutually dispersed complex, which is conducive to maximizing the synergistic effect of each component, effectively enhancing the therapeutic effect, and obtaining an amino acid chelated trace element composite package;

[0065] S2. Slowly add the amino acid chelated trace element composite package described in step S1 into the peony seed oil nanoemulsion, stir magnetically for 20 minutes, and then freeze-dry at a drying temperature of -30°C for a drying time of 8 hours. The amino acid chelated trace element composite package can be adsorbed on the surface structure of the peony seed oil nanoemulsion, further promoting the absorption of elements and nutrients. Then, the composite package is evenly mixed with corn flour and granulated to obtain a lactating sow compound feed.

[0066] Example 3

[0067] This embodiment provides a lactating sow compound feed, comprising the following components in parts by weight: 40 parts of peony seed oil nanoemulsion, 9 parts of amino acid chelated trace element composite package, and 85 parts of corn flour.

[0068] The peony seed oil nanoemulsion comprises the following components in parts by weight: 10 parts of peony seed oil, 15 parts of modified attapulgite, 15 parts of isolated soy protein, 25 parts of trehalose and 12.5 parts of chitosan hydrochloride.

[0069] The amino acid chelated trace element composite package comprises the following components in parts by weight: 15 parts of amino acid chelated iron, 15 parts of Chinese herbal medicine powder, and 12.5 parts of Bacillus subtilis.

[0070] The raw materials of the Chinese herbal medicine powder include the following components in parts by weight: 4 parts of astragalus, 4 parts of dandelion and 4 parts of honeysuckle.

[0071] The preparation method of peony seed oil nanoemulsion specifically comprises the following steps:

[0072] (1) Disperse 20 g of attapulgite into 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add monohydrated citric acid to adjust the pH to 3.0, stir magnetically at room temperature for 9 h, centrifuge at a speed of 6000 rpm, and centrifuge for 7.5 min. Wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush. Acidification can change the charge and adsorption activity of attapulgite, help depolymerize mineral aggregates, and make it more dispersible. In addition, attapulgite also contains major and trace elements necessary for livestock and poultry, such as copper, zinc, manganese, iron, cobalt, molybdenum and selenium, which are essential for the growth and development of animals, can improve the body's immune and antioxidant functions, and improve the nutrient utilization rate of feed and animal production performance, thereby obtaining modified attapulgite;

[0073] (2) Dissolve 0.15 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.25 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution. The amount of soy protein isolate added is 0.15 g. The soy protein isolate not only provides the required protein substance and supports the basic nutritional needs of animals during lactation, but also has the function of promoting lactation. Stir magnetically at room temperature for 1.5 hours, and hydrate in a refrigerator at 4°C for 10 hours. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 700 W and the ultrasonic time is 12.5 min. The ultrasonic treatment also improves the foaming performance of the soy protein isolate, which is beneficial to emulsification, to obtain an aqueous phase solution.

[0074] (3) Add peony seed oil dropwise into the aqueous phase solution described in step (2), wherein the amount of peony seed oil added is 4 mL. Peony seed oil has a special aromatic smell, which can stimulate the secretion of saliva and amylase in lactating sows, inhibit the number of harmful intestinal bacteria, increase the height of intestinal villi, and promote the digestion and absorption of nutrients, thereby promoting the production of nutrients such as milk protein, lactose, and milk solids in milk, thereby increasing the milk production and milk quality of sows. In the aqueous phase solution, modified attapulgite is used as a stabilizing carrier, soy protein isolate is used as an emulsifier, and trehalose promotes soy protein isolate to stabilize more oil-water interfaces, thereby reducing the flocculants of the nanoemulsion during the homogenization process. The coagulation and aggregation degree ensured the uniformity and stability of the particle size. The crude emulsion was obtained by high-speed dispersion at a speed of 11000 rpm for 4 min. The crude emulsion was then mixed with a 7.5% by mass chitosan hydrochloride aqueous solution, the pH was adjusted to 5.3, stirred at 52.5 ° C for 2.5 h, and then homogenized at high pressure at 25 MPa for 4 min to form a spherical granular coating. Chitosan hydrochloride as a wall material further enhanced the stability and utilization of peony seed oil, had mucosal adhesion and positive charge characteristics, could promote intestinal absorption of nutrients and the proliferation and differentiation of mammary epithelial cells, and obtained peony seed oil nanoemulsion.

[0075] The present embodiment provides a method for preparing a lactating sow compound feed, which specifically comprises the following steps:

[0076] S1. Astragalus, dandelion and honeysuckle were first crushed with a sieve aperture of 70 mesh, and then ultrafinely crushed with a sieve aperture of 250 mesh. Chinese herbal medicines contain a large number of functional active ingredients, which can improve the lactation performance and milk quality of sows, and provide some biologically active substances with anti-inflammatory, bactericidal and immunity-enhancing effects through breast milk, which is of great significance for maintaining the early healthy growth of piglets. The collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis were placed in a ball mill and ground for 25 minutes, with a controlled speed of 3000rpm. The activity of Bacillus subtilis was 1.5×10 6 cfu / g, the presence of Bacillus subtilis can degrade the cell wall in Chinese herbal medicine, so that the effective ingredients therein are precipitated in the digestive tract, and can also promote the absorption and utilization of amino acid chelated iron in the intestine, enhance nutrition and lactation performance, and the Chinese herbal medicine powder of tonifying the middle and replenishing qi promotes the proliferation and metabolism of Bacillus subtilis and plays a prebiotic role. The three form a mutually dispersed complex, which is conducive to maximizing the synergistic effect of each component, effectively enhancing the therapeutic effect, and obtaining an amino acid chelated trace element composite package;

[0077] S2. Slowly add the amino acid chelated trace element composite package described in step S1 into the peony seed oil nanoemulsion, stir magnetically for 25 minutes, and then freeze-dry at a drying temperature of -35°C for a drying time of 9 hours. The amino acid chelated trace element composite package can be adsorbed on the surface structure of the peony seed oil nanoemulsion, further promoting the absorption of elements and nutrients. Then, mix evenly with corn flour, granulate, and obtain a lactating sow compound feed.

[0078] Example 4

[0079] This embodiment provides a lactating sow compound feed, comprising the following components in parts by weight: 30 parts of peony seed oil nanoemulsion, 10 parts of amino acid chelated trace element composite package, and 90 parts of corn flour.

[0080] The peony seed oil nanoemulsion comprises the following components in parts by weight: 12 parts of peony seed oil, 20 parts of modified attapulgite, 20 parts of isolated soy protein, 20 parts of trehalose and 10 parts of chitosan hydrochloride.

[0081] The amino acid chelated trace element composite package comprises the following components in parts by weight: 20 parts of amino acid chelated iron, 10 parts of Chinese herbal medicine powder, and 15 parts of Bacillus subtilis.

[0082] The raw materials of the Chinese herbal medicine powder include the following components in parts by weight: 5 parts of astragalus, 5 parts of dandelion and 5 parts of honeysuckle.

[0083] The preparation method of peony seed oil nanoemulsion specifically comprises the following steps:

[0084] (1) Disperse 20 g of attapulgite in 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add monohydrated citric acid to adjust the pH to 3.0, stir magnetically at room temperature for 8 h, centrifuge at a speed of 7000 rpm for 5 min, wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush. Acidification can change the charge and adsorption activity of attapulgite, help depolymerize mineral aggregates, and make it more dispersible. In addition, attapulgite also contains major and trace elements necessary for livestock and poultry, such as copper, zinc, manganese, iron, cobalt, molybdenum and selenium, which are essential for the growth and development of animals, can improve the body's immune and antioxidant functions, and improve the nutrient utilization rate of feed and animal production performance, thereby obtaining modified attapulgite;

[0085] (2) Dissolve 0.2 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.2 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution. The amount of soy protein isolate added is 0.2 g. The soy protein isolate not only provides the required protein substance and supports the basic nutritional needs of animals during lactation, but also has the function of promoting lactation. Stir magnetically at room temperature for 1 hour, and then hydrate in a refrigerator at 4°C for 8 hours. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 800 W and the ultrasonic time is 10 min. The ultrasonic treatment also improves the foaming performance of the soy protein isolate, which is beneficial to emulsification, to obtain an aqueous phase solution.

[0086] (3) Add peony seed oil dropwise into the aqueous solution of step (2), wherein the amount of peony seed oil added is 5 mL. Peony seed oil has a special aromatic smell, which can stimulate the secretion of saliva and amylase in lactating sows, inhibit the number of harmful intestinal bacteria, increase the height of intestinal villi, and promote the digestion and absorption of nutrients, thereby promoting the production of nutrients such as milk protein, lactose, and milk solids in milk, thereby increasing the milk production and milk quality of sows. In the aqueous solution, modified attapulgite is used as a stabilizing carrier, soy protein isolate is used as an emulsifier, and trehalose promotes soy protein isolate to stabilize more oil-water interfaces, thereby reducing the effect of the nanoemulsion in the homogenization process. The flocculation and agglomeration degree in the mixture ensured the uniformity and stability of the particle size. The crude emulsion was obtained by high-speed dispersion at a speed of 12000 rpm for 3 min. The crude emulsion was then mixed with a 5% by mass chitosan hydrochloride aqueous solution, the pH was adjusted to 5.5, and the mixture was stirred at 55 °C for 2 h. Then, the mixture was homogenized at high pressure at 30 MPa for 3 min to form a spherical granular coating. Chitosan hydrochloride as a wall material further enhanced the stability and utilization of peony seed oil, had mucosal adhesion and positive charge characteristics, and could promote the intestinal absorption of nutrients and the proliferation and differentiation of mammary epithelial cells to obtain peony seed oil nanoemulsion.

[0087] The present embodiment provides a method for preparing a lactating sow compound feed, which specifically comprises the following steps:

[0088] S1. Astragalus, dandelion and honeysuckle were first crushed with a mesh size of 80 mesh, and then ultrafinely crushed with a mesh size of 300 mesh. Chinese herbal medicines contain a large number of functional active ingredients, which can improve the lactation performance and milk quality of sows, and provide some biologically active substances with anti-inflammatory, bactericidal and immunity-enhancing effects through breast milk, which is of great significance for maintaining the early healthy growth of piglets. The collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis were placed in a ball mill and ground for 20 minutes, with a controlled speed of 4000rpm. The activity of Bacillus subtilis was 2.0×10 6cfu / g, the presence of Bacillus subtilis can degrade the cell wall in Chinese herbal medicine, so that the effective ingredients therein are precipitated in the digestive tract, and can also promote the absorption and utilization of amino acid chelated iron in the intestine, enhance nutrition and lactation performance, and the Chinese herbal medicine powder of tonifying the middle and replenishing qi promotes the proliferation and metabolism of Bacillus subtilis and plays a prebiotic role. The three form a mutually dispersed complex, which is conducive to maximizing the synergistic effect of each component, effectively enhancing the therapeutic effect, and obtaining an amino acid chelated trace element composite package;

[0089] S2. Slowly add the amino acid chelated trace element composite package described in step S1 into the peony seed oil nanoemulsion, stir magnetically for 20 minutes, and then freeze-dry at a drying temperature of -40°C for a drying time of 8 hours. The amino acid chelated trace element composite package can be adsorbed on the surface structure of the peony seed oil nanoemulsion, further promoting the absorption of elements and nutrients. Then, mix evenly with corn flour, granulate, and obtain a lactating sow compound feed.

[0090] Comparative Example 1

[0091] This comparative example provides a lactating sow compound feed, which is different from Example 1 in that the peony seed oil nanoemulsion does not contain modified attapulgite and chitosan hydrochloride; the preparation method of the peony seed oil nanoemulsion does not include step (1); and the preparation method of the lactating sow compound feed is the same as that of Example 1.

[0092] Comparative Example 2

[0093] This comparative example provides a lactating sow compound feed, which is different from Example 1 in that the peony seed oil nanoemulsion does not contain peony seed oil; the preparation method of the peony seed oil nanoemulsion is the same as that of Example 1; the preparation method of the lactating sow compound feed is the same as that of Example 1.

[0094] Comparative Example 3

[0095] This comparative example provides a lactating sow compound feed, which is different from Example 1 in that the lactating sow compound feed does not contain an amino acid chelated trace element composite package; the preparation method of peony seed oil nanoemulsion is the same as that of Example 1; and the preparation method of lactating sow compound feed does not include step S1.

[0096] Experimental Example 1

[0097] Milk production experiment

[0098] Test sample: the lactating sow compound feed prepared in Examples 1-4 and Comparative Examples 1-3.

[0099] Test method: 35 lactating sows with the same parity and similar body weight in good health were selected and randomly divided into 7 groups, 5 in each group, to ensure the same feeding and management conditions for the test sows in each group. The test samples were added to the basic feed in a ratio of 7:3 for mixed feeding. The sows were fed once a day at 7:00, 12:00 and 18:00, and the feed was provided at 110% of the daily feed intake. After feeding for 7 days, the average daily milk production within 1 hour was calculated by testing the body weight of the sows. Each lactating sow and its suckling piglets (5 pigs) were taken as an observation unit. At 9:00 am on each test day, the piglets were separated from the lactating sows for 4 hours, and then the sows and piglets were put together for lactation. No food or water was allowed during the 1-hour lactation period. The body weight of the sows before and after lactation was weighed, and the difference was the milk production (g) of the sow in this 1 hour.

[0100] Figure 3 It is a milk production result diagram of Examples 1-4 and Comparative Examples 1-3; as shown in the figure, the milk production of Examples 1-4 is 508-550g, indicating that the milk production is large and lactation is effectively promoted; the milk production of Comparative Examples 1-3 is 420-465g, indicating that the milk production is small and lactation cannot be effectively promoted; the peony seed oil nanoemulsion of Comparative Example 1 does not contain modified attapulgite and chitosan hydrochloride, which reduces the stability of peony seed oil, is not conducive to its role in promoting milk secretion, resulting in less milk production and ineffective promotion of lactation; the peony seed oil nanoemulsion of Comparative Example 2 does not contain peony seed oil and lacks effective substances, resulting in less milk production and ineffective promotion of lactation; the lactating sow compound feed of Comparative Example 3 does not contain an amino acid chelated trace element composite package, which is not conducive to further promoting the absorption and utilization of other effective ingredients such as peony seed oil, resulting in less milk production and ineffective promotion of lactation.

[0101] Experimental Example 2

[0102] Milk nutritional composition experiment

[0103] Test sample: the lactating sow compound feed prepared in Examples 1-4 and Comparative Examples 1-3.

[0104] Test method: 35 lactating sows with the same parity and similar body weight in good health were selected and randomly divided into 7 groups, with 5 sows in each group, to ensure that the feeding and management conditions of each group of experimental sows were consistent, and the test samples were added to the basic feed in a ratio of 7:3 for mixed feeding. The feed was fed once a day at 7:00, 12:00 and 18:00, at 110% of the daily feed intake. After feeding for 14 days, the sows' milk was collected, and the content of milk protein, lactose and milk solids in the milk was detected using a high-precision infrared breast milk analyzer.

[0105] Figure 4The result diagram of the nutritional components of milk of Examples 1-4 and Comparative Examples 1-3; as shown in the figure, the contents of milk protein, lactose and milk solids in the milk of Examples 1-4 are 11-15%, 5-6% and 33-38%, respectively, indicating that the nutritional value of the milk is relatively high; the contents of milk protein, lactose and milk solids in the milk of Comparative Examples 1-3 are 5-8%, 3-4% and 20-25%, respectively, indicating that the nutritional value of the milk is relatively low; the peony seed oil nanoemulsion of Comparative Example 1 does not contain modified attapulgite and chitosan hydrochloride, which is not conducive to the stability and absorption and utilization of peony seed oil, thereby affecting the efficacy of peony seed oil, resulting in a low nutritional value of the milk; the peony seed oil nanoemulsion of Comparative Example 2 does not contain peony seed oil, lacks the effect of promoting the generation of nutrients by effective substances, resulting in a low nutritional value of the milk; the lactating sow compound feed of Comparative Example 3 does not contain an amino acid chelated trace element composite package, which is not conducive to the coordinated effect of peony seed oil nanoemulsion, resulting in a low nutritional value of the milk.

[0106] Experimental Example 3

[0107] Lactation performance test

[0108] Test sample: the lactating sow compound feed prepared in Examples 1-4 and Comparative Examples 1-3.

[0109] Test method: 35 lactating sows of the same parity and similar body weight in good health were selected and randomly divided into 7 groups, 5 sows in each group, to ensure the same feeding and management conditions for the test sows in each group, add the test sample to the basic feed in a ratio of 7:3 and feed them mixedly at 7:00, 12:00 and 18:00 each day, feeding at 110% of the daily feed intake for 21 days. Each lactating sow and its suckling piglets (10) were taken as an observation unit, and the daily weight gain (g) and diarrhea rate (%) of the piglets were recorded and calculated.

[0110] Figure 5The lactation performance results of Examples 1-4 and Comparative Examples 1-3 are shown in FIG. 1 ; as shown in the figure, the daily weight gain and diarrhea rate of the piglets in Examples 1-4 are 205-222 g and 10-20%, respectively, indicating that the milk quality is good and the lactation performance is good; the daily weight gain and diarrhea rate of the piglets in Comparative Examples 1-3 are 171-190 g and 50-70%, respectively, indicating that the milk quality is general and the lactation performance is poor; the peony seed oil nanoemulsion in Comparative Example 1 does not contain modified attapulgite and chitosan hydrochloride, which is not conducive to the synthesis of peony seed oil and amino acid chelated trace elements. The absorption and utilization of the amino acid chelated trace element composite package of peony seed oil in comparative example 3 cannot effectively promote the milk secretion and the production of nutrients, resulting in average milk quality and poor lactation performance; the peony seed oil nanoemulsion in comparative example 2 does not contain peony seed oil, and cannot effectively promote the milk secretion and the production of nutrients, resulting in average milk quality and poor lactation performance; the lactating sow compound feed in comparative example 3 does not contain the amino acid chelated trace element composite package, and cannot further enhance the stability of peony seed oil, nor can it be compounded with peony seed oil to enhance the promoting effect on milk secretion and nutrients, resulting in average milk quality and poor lactation performance.

[0111] The above experimental results show that the milk yield and milk quality of Examples 1-4 of the present invention are significantly better than those of Comparative Examples 1-3. Among them, the milk yield and milk quality of Example 1 using peony seed oil nanoemulsion and amino acid chelated trace element composite package are higher. In the peony seed oil nanoemulsion, the aqueous phase solution formed by modified attapulgite, trehalose and soy protein isolate wraps the peony seed oil, and chitosan hydrochloride is used as the wall material, which enhances the stability of the peony seed oil and increases the residence time in the intestinal mucosa, which is beneficial to the absorption and utilization of each component. The amino acid chelated trace element composite package forms a mutually dispersed block complex, which is adsorbed on the surface network structure of the peony seed oil nanoemulsion. The composite combination of the composite package and the nanoemulsion helps to improve the intestinal function of the sow, thereby helping to improve the lactation performance.

[0112] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

[0113] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A lactating sow compound feed, characterized in that: The invention comprises the following components in parts by weight: 30-50 parts of peony seed oil nanoemulsion, 8-10 parts of amino acid chelated trace element composite package, and 80-90 parts of corn flour; the peony seed oil nanoemulsion is made of the following components in parts by weight: 8-12 parts of peony seed oil, 10-20 parts of modified attapulgite, 10-20 parts of soy protein isolate, 20-30 parts of trehalose, and 10-15 parts of chitosan hydrochloride; the amino acid chelated trace element composite package comprises the following components in parts by weight: 10-20 parts of amino acid chelated iron, 10-20 parts of Chinese herbal medicine powder, and 10-15 parts of Bacillus subtilis; the raw materials of the Chinese herbal medicine powder comprise the following components in parts by weight: 3-5 parts of astragalus, 3-5 parts of dandelion, and 3-5 parts of honeysuckle; The preparation method of the peony seed oil nanoemulsion comprises the following steps: (1) Disperse 20 g of attapulgite into 500 mL of deionized water, add 0.8 g of sodium hexametaphosphate to remove impurities, stir at room temperature for 8 h, let stand overnight, take the upper suspension, add citric acid monohydrate to adjust the pH to 3.0, stir magnetically at room temperature for 8-10 h, centrifuge at a speed of 5000-7000 rpm for 5-10 min, wash the precipitate with deionized water until the pH of the supernatant reaches 7.0, dry, and crush to obtain modified attapulgite; (2) Dissolve 0.1-0.2 g of the modified attapulgite described in step (1) in 30 mL of water for standby use, dissolve 0.2-0.3 g of trehalose in 30 mL of PBS buffer solution, and then add soy protein isolate to disperse in the trehalose solution, stir magnetically at room temperature for 1-2 h, and hydrate in a refrigerator at 4°C for 8-12 h. The obtained protein trehalose solution and the modified attapulgite solution are ultrasonically homogenized. During the ultrasonic process, the ultrasonic power is 600-800 W and the ultrasonic time is 10-15 min to obtain an aqueous phase solution; (3) dropping peony seed oil into the aqueous phase solution described in step (2), dispersing at high speed to obtain a crude emulsion, the rotation speed is 10000-12000 rpm, the time is 3-5 min, and then mixing the crude emulsion with a chitosan hydrochloride aqueous solution with a mass fraction of 5-10%, adjusting the pH to 5.0-5.5, stirring at 50-55° C. for 2-3 h, and then high-pressure homogenizing at 20-30 MPa for 3-5 min to obtain a peony seed oil nanoemulsion.

2. A method for preparing a lactating sow compound feed according to claim 1, characterized in that: The specific steps include: S1. Grind astragalus, dandelion and honeysuckle first, with a mesh size of 60-80 mesh, and then ultrafine grind with a mesh size of 200-300 mesh. Grind the collected Chinese herbal medicine powder, amino acid chelated iron and Bacillus subtilis in a ball mill for 20-30 minutes, and control the rotation speed to 2000-4000rpm to obtain an amino acid chelated trace element composite package; S2. Slowly add the amino acid chelated trace element composite package described in step S1 into the peony seed oil nanoemulsion, stir magnetically for 20-30 minutes, and then freeze-dry at a drying temperature of -30°C to -40°C for 8-10 hours, and then mix evenly with corn flour and granulate to obtain a lactating sow compound feed.

3. The method for preparing a lactating sow compound feed according to claim 2, characterized in that: In step S1, the activity of Bacillus subtilis was 1.0-2.0×10 6 cfu / g.

4. The lactating sow compound feed according to claim 1, characterized in that: In step (2), the amount of soy protein isolate added is 0.1-0.2 g.

5. The lactating sow compound feed according to claim 1, characterized in that: In step (3), the amount of peony seed oil added is 3-5 mL.

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