Application of Miao medicine bone setting tea fresh medicine in preparation of anti-inflammatory and analgesic medicine

Abstract

The invention discloses an application of a Miao medicine bone-setting tea fresh medicine in preparation of an anti-inflammatory and analgesic medicine, and relates to the technical field of biological medicines, and the technical key points are as follows: through carrying out anti-inflammatory, analgesic and swelling inhibition experiments on mice by using a glabrous sarcandra herb alcohol extract, the glabrous sarcandra herb alcohol extract has anti-inflammatory, analgesic and swelling inhibition effects, and the glabrous sarcandra herb alcohol extract has the anti-inflammatory, analgesic and swelling inhibition effects. The compound can be applied to anti-inflammatory and analgesic drugs.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to application of fresh Miao medicine bone-setting tea in the preparation of anti-inflammatory and analgesic drugs. Background Art

[0002] Due to inflammatory factors, tissue fluid exudation, increased basal metabolic rate, capsule irritation and other reasons, the human body will experience pain symptoms after inflammation. Inflammatory factors such as prostaglandins and bradykinin stimulate local nerves, causing pain; tissue fluid exudation may cause local swelling and compress nerves, causing pain; increased basal metabolic rate may cause fever and stimulate local nerves to cause pain; capsule irritation, such as swelling in hepatitis, stimulates the capsule or subcutaneous nerves, causing pain.

[0003] Sarcandra glabra (Thunb.) Nakai. is a common medicine in the Miao area. Its Miao name is "Det nixvob dliob" (a tree or vegetable with green leaves that stretch out like indigo). It is also known as "bone-jointing tea", "swelling knotweed", "coral grass", etc. It mainly contains coumarins, organic acids, flavonoids and other ingredients. Toxicity studies have shown that it is safe and non-toxic and can be used as an edible medicinal material without mutagenicity. "Guizhou Herbal Medicine" records that "bone-jointing tea, four tiles, and blood-removing grass, each five cents, stewed with wine to treat back pain caused by fatigue". As a common folk medicine of the Miao people, it is usually fresh and crushed for external application to treat fractures, traumatic injuries, etc., with significant efficacy.

[0004] Jiujie tea has antibacterial and anti-inflammatory, antiviral, immune regulation, anti-tumor, liver protection, and thrombocytopenia inhibition effects, but research on the application of Jiujie tea in anti-inflammatory and analgesic drugs is still relatively rare. Summary of the invention

[0005] The purpose of the present invention is to solve the above problems and provide a use of fresh Miao medicine bone-setting tea in the preparation of anti-inflammatory and analgesic drugs.

[0006] In order to achieve the above object, the technical solution of the present invention is as follows:

[0007] The invention provides an application of a fresh Miao medicine bone-setting tea in the preparation of an anti-inflammatory and analgesic drug, comprising: the fresh Miao medicine bone-setting tea is fresh Jiujiecha tea powder.

[0008] The present invention is further configured as follows: taking 1 portion of fresh powder of Camellia ternata, adding 3 times the amount of 75% ethanol, soaking for 48 hours, filtering, adding 3 times the amount of 75% ethanol again, soaking for 24 hours, filtering, combining the filtrate, and concentrating the filtrate under reduced pressure in a 50°C water bath to extract the Camellia ternata alcohol extract.

[0009] The present invention is further configured as follows: the alcohol extract of the nine-section tea can relieve pain and inhibit swelling.

[0010] Compared with the prior art, this solution has the following beneficial effects:

[0011] 1. Toxicity studies have shown that it is safe and non-toxic, can be used as an edible medicinal material, is non-mutagenic, and is safe and reliable.

[0012] 2. The alcohol extract of Jiujiecha has strong anti-inflammatory and analgesic activity, and its high-dose group has a higher efficacy than the aspirin positive control group. DETAILED DESCRIPTION

[0013] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described in detail below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work should fall within the scope of protection of the present invention.

[0014] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present invention can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0015] Example:

[0016] 1. Pharmacological test methods

[0017] 1.1 Preparation of Jiujiecha alcohol extract (hereinafter referred to as ZX-2) Take 1.85kg of Jiujiecha fresh powder, add 3 times the amount of 75% ethanol, soak for 48h, filter, add 3 times the amount of 75% ethanol again, soak for 24h, filter, combine the filtrate, and concentrate the filtrate under reduced pressure in a 50℃ water bath to a liquid concentration of 8.565g / mL (fresh drug amount). Set low, medium and high dose groups as: 1.071g / mL, 2.141g / mL, 4.282g / mL. During the experiment, 0.4mL / 20g was used for gavage administration. The dosage of low, medium and high dose groups were: 21.412g / kg, 42.824g / kg, 85.648g / kg (fresh drug concentration), respectively.

[0018] 1.2 Control group setting

[0019] Blank control group: physiological saline, 0.4mL / 20g was administered by gavage during the experiment.

[0020] Positive drug group: Aspirin group, grind the tablets into powder, prepare a suspension with a concentration of 10mg / mL, and dissolve it by ultrasound for 30min. During the test, 0.4mL / 20g was administered by gavage. Dexamethasone group: According to the dosage requirements of the dosage (0.015g / Kg) and the dosage (20ml / Kg), grind the 0.75mg / tablet dexamethasone acetate tablets, add appropriate amount of water, and prepare a suspension of 1mg / mL for use.

[0021] 1.3 Effects of acetic acid on writhing response in mice

[0022] 50 Kunming mice, half male and half female, weighing (20±2)g, were randomly divided into 5 groups, namely, saline (20mL / kg) group, aspirin (positive drug, 200mg / kg) group, Jiujiecha low-dose group (21.412g / kg), Jiujiecha medium-dose group (42.824g / kg), Jiujiecha high-dose group (85.648g / kg) (fresh drug concentration). Each group had 10 mice, half male and half female. The mice were gavaged with 0.4mL per 20g body weight, once a day, for 5 consecutive days. 30min after the last administration, the mice were intraperitoneally injected with 0.6% glacial acetic acid 0.25mL, and the number of twists of the mice within 15min was recorded.

[0023] The writhing reaction inhibition rate (%) = (average number of writhing times in the saline group - average number of writhing times in the drug-treated group) / average number of writhing times in the saline group × 100%.

[0024] 1.4 Effect of xylene on ear swelling in mice

[0025] Fifty Kunming mice, half male and half female, weighing (20±2) g, were randomly divided into 8 groups, namely, saline (20 mL / kg) group, dexamethasone (positive drug, 20 mg / kg) group, Jiujiecha low-dose group (21.412 g / kg), Jiujiecha medium-dose group (42.824 g / kg), Jiujiecha high-dose group (85.648 g / kg) (fresh drug concentration). Each group had 10 mice, half male and half female, and the mice were gavaged, 0.4 mL per 20 g body weight, once a day, for 5 consecutive days. 40 minutes after the last administration, 30 μL of xylene was applied to the right ear of the mouse to induce inflammation. 70 minutes after the last administration, the mice were killed by cervical dislocation, the two ears were cut off along the base of the auricle, and a round ear piece was punched at the same position of the left and right ears with a 9 mm diameter puncher, and the swelling degree of the auricle and the inhibition rate were calculated.

[0026] Auricle swelling inhibition rate (%) = (average swelling rate of saline group - average swelling rate of test group) / average swelling rate of saline group × 100%.

[0027] 1.5 Statistics

[0028] SPSS17.0 software was used to perform statistical analysis on the experimental data. The experimental measurement data were expressed as "x±s". The two-sample t-test was used to compare the differences between the groups, and the one-way analysis of variance was used to compare the means among multiple groups. P<0.05 was considered statistically significant.

[0029] 2. Pharmacological test results

[0030] 2.1 Effect of the alcohol extract of Camellia ternata on acetic acid-induced writhing reaction in mice

[0031] Compared with the normal saline group, the number of writhing reactions of mice in the aspirin (positive drug) group was extremely significantly reduced (P<0.01), and the pain inhibition rate reached 67.63%; the analgesic effect of the drug group increased with the increase of drug concentration, and the low, medium and high dose groups of Jiujiecha alcohol extract all significantly reduced the number of writhing reactions of mice (P<0.01), and the pain inhibition rates reached 61.27%, 63.87% and 73.41%, respectively, as shown in Table 1.

[0032] Table 1 Effects of ZX-2 on writhing reaction induced by acetic acid in mice

[0033]

[0034] Note: 1. -- indicates that the inhibition rate was not calculated; 2. The ** in the same column indicates that the difference is extremely significant (p<0.01) compared with the normal saline group, and the * indicates that the difference is extremely significant (P<0.05).

[0035] 2.2 Effect of the alcohol extract of Camellia sinensis on xylene-induced ear swelling in mice

[0036] Compared with the saline group, the dexamethasone group could significantly reduce the swelling of the mouse auricle (P<0.05), and the swelling inhibition rate was 51.13%; the ethanol extract of Jiujiecha had a certain effect of inhibiting the swelling of the mouse auricle, and the inhibitory effect increased with the increase of drug concentration. The high-dose group of ethanol extract of Jiujiecha could significantly inhibit the swelling of the mouse auricle (P<0.05), and the swelling inhibition rate was 46.37%, as shown in Table 2.

[0037] Table 4 Effects of ZX-2 on xyleneinduced auricle swelling in mice

[0038]

[0039] Note: 1.— indicates that the inhibition rate was not calculated; 2. The data in the same column with an * indicates that the difference is significant compared with the saline group (P<0.05); the data with a ** indicates that the difference is extremely significant compared with the saline group (P<0.01).

[0040] The above specific embodiments are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, such modifications are protected by the patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A use of fresh Miao medicine bone-setting tea in the preparation of anti-inflammatory and analgesic drugs, characterized in that: The fresh medicine of Miao medicine bone-setting tea is fresh medicine powder of Jiujiecha.

2. The use of the fresh Miao medicine bone-setting tea as claimed in claim 1 in the preparation of anti-inflammatory and analgesic drugs, characterized in that: Take 1 portion of fresh powder of Camellia ternata, add 3 times the amount of 75% ethanol, soak for 48 hours, filter, add 3 times the amount of 75% ethanol again, soak for 24 hours, filter, combine the filtrate, and concentrate the filtrate under reduced pressure in a 50°C water bath to extract the Camellia ternata alcohol extract.

3. The use of the fresh Miao medicine bone-setting tea as claimed in claim 2 in the preparation of anti-inflammatory and analgesic drugs, characterized in that: The ethanol extract of the nine-section tea can relieve pain and inhibit swelling.

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