A Chinese medicine preparation for treating gastric disease and its preparation method and application

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and specifically relates to a Chinese medicine preparation for treating gastric disease, and a preparation method and application thereof. The Chinese medicine preparation comprises the following raw materials in parts by weight: 30-40 parts of white flower bitter lantern root, 25-35 parts of shrimp grass, 10-20 parts of dried orange peel, 12-18 parts of immature bitter orange, 6-10 parts of cucumber seeds, 8-10 parts of spiny jujube seeds, 2-5 parts of lily, and 1-3 parts of licorice. In the prescription, the white flower bitter lantern root promotes blood circulation and dredges the meridians, relieves swelling and relieves pain, and the shrimp grass dispels wind and clears heat, which are the main medicines; the dried orange peel regulates qi and strengthens the spleen, the immature bitter orange breaks qi and eliminates accumulation, and the cucumber seeds dispel wind and strengthen the stomach, promote digestion, which are the minister medicines; the spiny jujube seeds nourish the heart and calm the mind, and the lily nourishes yin and produces body fluid, which are the adjuvant medicines; and the licorice is used as the adjuvant to coordinate the various medicines. The whole prescription is reasonably compatible and treats atrophic gastritis together.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine preparation for treating gastric diseases and a preparation method and application thereof. Background Art

[0002] Chronic atrophic gastritis (CAG), also known as atrophic gastritis, is a chronic digestive system disease characterized by atrophy of gastric mucosal epithelium and glands, thinning of gastric mucosa, thickening of mucosal basal layer, accompanied by pyloric metaplasia and intestinal metaplasia, or atypical hyperplasia. For this disease, the current treatment is mainly based on drug therapy, among which Western medicine occupies an important position, such as antacids and antibacterial drugs. Although such drugs can effectively relieve symptoms, long-term use will produce side effects, such as damage to liver and kidney function.

[0003] Traditional Chinese medicine believes that chronic atrophic gastritis is mostly caused by factors such as spleen and stomach weakness, internal dampness and heat, qi stagnation and blood stasis, and Chinese medicine can achieve the effect of treating both the symptoms and the root causes. Studies have found that Chinese medicine can not only improve the clinical symptoms of chronic atrophic gastritis, but also reverse intestinal metaplasia, improve gastric mucosal atrophy, and effectively prevent chronic atrophic gastritis from developing into gastric cancer. Based on this, the treatment of chronic atrophic gastritis with Chinese medicine has received increasing attention and concern.

[0004] At present, there are many traditional Chinese medicines for treating chronic atrophic gastritis, such as Chinese patent CN118750563A, which discloses a Chinese medicine composition for treating chronic atrophic gastritis. Chinese patent CN118078948A discloses a prescription for treating chronic atrophic gastritis. However, the above preparations all have problems such as unsatisfactory therapeutic effects. Therefore, it is of great significance to develop a safe and effective new traditional Chinese medicine preparation for treating chronic atrophic gastritis. Summary of the invention

[0005] The first object of the present invention is to provide a Chinese medicine preparation for treating gastric diseases, which can effectively treat atrophic gastritis.

[0006] The second purpose of the present invention is to provide a method for preparing a Chinese medicine preparation for treating gastric disease, which is simple to operate.

[0007] The third purpose of the present invention is to provide an application of a traditional Chinese medicine preparation for treating gastric disease, which has broad application prospects.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is:

[0009] A traditional Chinese medicine preparation for treating gastric disease comprises the following raw materials in parts by weight: 30-40 parts of Radix Achyranthis Bidentatae, 25-35 parts of Herba Schizonepetae, 10-20 parts of dried tangerine peels, 12-18 parts of immature bitter oranges, 6-10 parts of cucumber seeds, 8-10 parts of spiny jujube seeds, 2-5 parts of lilies and 1-3 parts of liquorice.

[0010] Furthermore, the invention comprises the following raw materials in parts by weight: 37 parts of Radix Achyranthis Bidentatae, 29 parts of Herba Schizonepetae, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily and 2 parts of licorice.

[0011] The method for preparing the above-mentioned Chinese medicine preparation for treating gastropathy comprises the following steps:

[0012] 1) Weighing and mixing the raw materials according to the weight ratio, soaking, decocting and filtering to obtain a first decoction and a first filter residue;

[0013] 2) adding water to the first filter residue of step 1), soaking, decocting and filtering to obtain a second decoction; combining the first decoction and the second decoction, concentrating to an extract with a relative density of 1.10-1.25, and preparing the extract with pharmaceutically acceptable excipients into a corresponding dosage form.

[0014] Furthermore, the amount of water used in step 1) and step 2) is 10-15 times the total weight of the raw material.

[0015] Furthermore, in step 1), the soaking time is 1-2 hours, and the decocting time is 2-3 hours.

[0016] Furthermore, in step 2), the soaking time is 2-3 hours, and the decocting time is 3-4 hours.

[0017] Furthermore, the dosage form is an oral dosage form.

[0018] Furthermore, the oral dosage form is tablets, granules, capsules or oral liquid.

[0019] Application of the above-mentioned traditional Chinese medicine preparation for treating gastric diseases in preparing medicine for treating atrophic gastritis.

[0020] The pharmacological properties of each raw material of the present invention are analyzed as follows:

[0021] White flower bitter lantern root: cool in nature, slightly bitter in taste. Clears away heat and detoxifies, dispels wind and dampness, and nourishes the spleen and kidneys; mainly treats colds and fever, headaches, coughs, back and leg pain, nephritis edema, and rheumatic joint pain.

[0022] Shrimp beard grass: bitter, neutral, and belongs to the heart meridian. It has the effects of clearing heat and detoxifying, promoting diuresis and reducing swelling. It is mainly used to treat sores, swelling, edema, and wind-heat headache. "Guizhou Herbal Medicine" records: clearing heat and detoxifying, promoting diuresis and reducing swelling, and dispersing wind.

[0023] Tangerine peel: bitter, pungent, warm in nature, enters the lung and spleen meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. It is mainly used to treat abdominal distension, loss of appetite, vomiting and diarrhea, and cough with excessive phlegm.

[0024] Citrus aurantium: bitter, pungent, sour, slightly cold, enters the spleen and stomach meridians. It can dissipate qi and eliminate stagnation, resolve phlegm and relieve lumps. It is mainly used to treat stagnation of internal organs, fullness and pain, heaviness after diarrhea, constipation, phlegm stagnation and qi obstruction, chest pain, chest congestion, and sagging of internal organs.

[0025] Cucumber seeds: sweet in taste, cool in nature. It has the effects of calcium supplementation, wind dispelling, phlegm elimination, tendon and bone repair. It is mainly used to treat rheumatism, phlegm and asthma in the elderly, fractures and tendon injuries.

[0026] Ziziphus jujuba seed: sweet, sour, neutral in nature, enters the liver, gallbladder, and heart meridians. It has the effects of nourishing the heart and liver, calming the mind, astringing sweat, and promoting body fluid. It is mainly used to treat insomnia, palpitations, thirst, and sweating.

[0027] Lily: sweet in taste, cold in nature, enters the heart and lung meridians. It has the effects of nourishing yin and moistening the lungs, clearing the heart and calming the mind. It is mainly used to treat dry cough due to yin deficiency, cough due to fatigue and hemoptysis, insomnia and palpitations due to yin deficiency and heat, and lily disease syndrome of heart and lung yin deficiency and internal heat.

[0028] Licorice: sweet, neutral in nature, enters the spleen, stomach, heart, and lung meridians. It can relieve urgency, moisten the lungs, clear away heat and detoxify, and harmonize various medicines. It is mainly used to treat shortness of breath, fatigue, poor appetite, loose stools, cough, sore throat, etc. "Ben Jing" records: It can treat cold and heat evil spirits in the five internal organs, strengthen tendons and bones, grow muscles, double strength, wounds and swellings, and detoxify.

[0029] The beneficial technical effects of the present invention are:

[0030] The invention provides a Chinese medicine preparation for treating gastric disease, which achieves the purpose of treating atrophic gastritis by promoting blood circulation and removing blood stasis, increasing strength, promoting qi and removing accumulation, strengthening the stomach and digesting food, nourishing yin and promoting body fluid, and being sweet and mild to penetrate dampness. Specifically, the root of white flower and bitter lantern in the prescription can promote blood circulation and dredge meridians, reduce swelling and relieve pain, and the herb of shrimp whiskers can dispel wind and clear heat, and they are all monarch medicines; the dried orange peel can regulate qi and strengthen the spleen, the immature bitter orange can break qi and eliminate accumulation, and the cucumber seed can dispel wind and strengthen the stomach and promote digestion, and they are all minister medicines; the Chinese jujube seed can nourish the heart and calm the mind, and the lily can nourish yin and promote body fluid, and they are all adjuvant medicines; and the licorice is used as a guiding medicine to coordinate the various medicines. The whole prescription is reasonably compatible and can treat atrophic gastritis together. DETAILED DESCRIPTION

[0031] The above contents of the present invention are further described in detail below in the form of embodiments, but this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples, and all technologies realized based on the above contents of the present invention belong to the scope of the present invention.

[0032] 1. Embodiment

[0033] Example 1

[0034] A traditional Chinese medicine preparation for treating gastric disease comprises the following raw materials in parts by weight: 37 parts of Radix Achyranthis Bidentatae, 29 parts of Herba Schizonepetae, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily and 2 parts of liquorice.

[0035] The preparation method of the above-mentioned Chinese medicine preparation for treating gastropathy, the specific preparation process is as follows:

[0036] 1) Weigh and mix the raw materials according to the weight ratio, use water in an amount of 12 times the total weight of the raw materials, soak in water for 1.5 hours, and then boil for 2.5 hours. After boiling, filter to obtain a first decoction and a first filter residue;

[0037] 2) adding water in an amount of 12 times the total weight of the raw material drug to the first filter residue of step 1), soaking it for 2.5 hours, then decocting it for 3.5 hours, filtering it after decocting to obtain a second decoction; combining the first decoction and the second decoction, concentrating it to an extract with a relative density of 1.13, drying and crushing the extract, and filling it into capsules as capsule powder to prepare capsules.

[0038] Example 2

[0039] A traditional Chinese medicine preparation for treating gastric disease comprises the following raw materials in parts by weight: 30 parts of Radix Achyranthis Bidentatae, 25 parts of Herba Schizonepetae, 10 parts of dried tangerine peel, 12 parts of immature bitter orange, 6 parts of cucumber seeds, 8 parts of spiny jujube seeds, 2 parts of lily and 1 part of liquorice.

[0040] The preparation method of the above-mentioned Chinese medicine preparation for treating gastropathy, the specific preparation process is as follows:

[0041] 1) Weigh and mix the raw materials according to the weight ratio, use water in an amount of 15 times the total weight of the raw materials, soak in water for 2 hours, and then boil for 3 hours. After boiling, filter to obtain a first decoction and a first filter residue;

[0042] 2) adding water in an amount of 15 times the total weight of the API to the first filter residue of step 1), soaking for 3 hours, decocting for 4 hours, filtering after decocting to obtain a second decoction; combining the first decoction and the second decoction, concentrating to an extract with a relative density of 1.10, adding xylitol to the extract, and preparing granules by conventional methods.

[0043] Example 3

[0044] A traditional Chinese medicine preparation for treating gastric disease comprises the following raw materials in parts by weight: 40 parts of Radix Achyranthis Bidentatae, 35 parts of Herba Schizonepetae, 20 parts of dried tangerine peel, 18 parts of immature bitter orange, 10 parts of cucumber seeds, 10 parts of spiny jujube seeds, 5 parts of lily and 3 parts of liquorice.

[0045] The preparation method of the above-mentioned Chinese medicine preparation for treating gastropathy, the specific preparation process is as follows:

[0046] 1) Weigh and mix the raw materials according to the weight ratio, use water in an amount of 10 times the total weight of the raw materials, soak in water for 1 hour, and then boil for 2 hours. After boiling, filter to obtain a first decoction and a first filter residue;

[0047] 2) adding water in an amount of 10 times the total weight of the API to the first filter residue of step 1), soaking for 2 hours, decocting for 3 hours, filtering after decocting to obtain a second decoction; combining the first decoction and the second decoction, concentrating to an extract with a relative density of 1.25, adding xylitol to the extract, and preparing an oral solution by a conventional method.

[0048] 2. Comparative Example

[0049] Comparative Example 1

[0050] The difference between Comparative Example 1 and Example 1 is that the Chinese medicine preparation comprises the following raw materials in parts by weight: 29 parts of Herba Lycopodii, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily, and 2 parts of licorice.

[0051] Comparative Example 2

[0052] The difference between Comparative Example 2 and Example 1 is that the Chinese medicine preparation comprises the following raw materials in parts by weight: 66 parts of Herba Lycopodii, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily, and 2 parts of licorice.

[0053] Comparative Example 3

[0054] The difference between Comparative Example 3 and Example 1 is that the Chinese medicine preparation comprises the following raw materials in parts by weight: 37 parts of Radix Achyranthis Bidentatae, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily, and 2 parts of licorice.

[0055] Comparative Example 4

[0056] The difference between Comparative Example 4 and Example 1 is that the Chinese medicine preparation comprises the following raw materials in parts by weight: 66 parts of Radix Achyranthis Bidentatae, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lily, and 2 parts of licorice.

[0057] Comparative Example 5

[0058] The difference between Comparative Example 5 and Example 1 is that the Chinese medicine preparation comprises the following raw materials in parts by weight: 37 parts of Radix Glehniae chinensis, 29 parts of Herba Lycopodii, 14 parts of dried tangerine peel, 13 parts of immature bitter orange, 9 parts of spiny jujube seeds, 4 parts of lily, and 2 parts of licorice.

[0059] The technical effects that can be achieved by the Chinese medicine preparation of the present invention are described below.

[0060] 3. Test examples

[0061] 3.1 Grouping and modeling

[0062] Experimental subjects: A total of 130 SD rats were selected, weighing 180-200g, half of which were male and half were female.

[0063] Grouping and modeling: 10 rats were taken as the normal control group, and the remaining 120 rats were orally administered with 60% ethanol solution once (on an empty stomach), 2 mL per rat each time, once every 5 days in the first month, once every 7 days in the second month, during which pH 7.0-7.8, 20mmol / L sodium deoxycholate solution was used to replace water for free drinking. In the third month, 30% ethanol solution and 20mmoL / L sodium deoxycholate solution were used, respectively, and water was freely drunk once every 3 days. During the 3-month period, hunger and satiety disorders were coordinated: 2 days of sufficient feeding, 1 day of fasting, and unlimited feeding days. The experimental time for the above model construction was 3 months. Before the end of the modeling, 6 rats were randomly selected for gastric mucosal pathological histological examination. After confirming that the model was successfully constructed, 100 model rats were randomly divided into a model group, a positive control group, a low-dose group in Example 1 of the present invention, a medium-dose group in Example 1, a high-dose group in Example 1, and a high-dose comparative example 1-5 group, with 10 rats in each group.

[0064] 3.2 Administration

[0065] Model group: intragastric administration of equal volume of distilled water;

[0066] Normal control group: gavage with an equal volume of distilled water;

[0067] Positive control group: Oral administration of Morodan at a dose of 0.36 g / kg;

[0068] Example 1 low-dose group: gavage of the Chinese medicine preparation of Example 1 at a dose of 5 g / kg (raw medicine);

[0069] The medium dose group in Example 1: gavage of the Chinese medicine preparation in Example 1 at a dose of 10 g / kg (raw drug);

[0070] Example 1 high-dose group: gavage of the Chinese medicine preparation of Example 1 at a dose of 20 g / kg (raw drug);

[0071] High-dose comparative example 1-5 group: The Chinese medicine preparations of comparative examples 1-5 were respectively gavaged at a dose of 20 g / kg (raw medicine).

[0072] Each group was given the drug continuously for 30 days according to the above-mentioned administration method. After the administration, the animals in each group were fasted but not watered for 24 hours, then anesthetized with 10% urethane, the abdomen was opened, the pylorus was ligated, and the abdominal cavity was closed by suture.

[0073] 3.3 Index detection

[0074] 3.3.1 Gastric juice analysis

[0075] The gastric juice of rats in each group was taken and the pepsin activity was detected by ELISA kit. The test results are shown in Table 1.

[0076] 3.3.2 Blood analysis

[0077] The serum of rats in each group was taken to detect the following indicators:

[0078] (1) Serum gastrin levels were tested according to the kit instructions. The test results are shown in Table 1.

[0079] (2) The TBA colorimetric method was used to detect the lipid peroxide (LPO) content in serum. The test results are shown in Table 2.

[0080] (3) The NBT method was used to detect the activity of superoxide dismutase (SOD) in serum. The test results are shown in Table 2.

[0081] (4) The DTNB method was used to detect the activity of glutathione peroxidase (GSH-Px) in serum. The test results are shown in Table 2.

[0082] Table 1

[0083]

[0084] Table 2

[0085]

[0086] As shown in Table 1, compared with the normal control group, the GAS and PP of the model group rats after modeling were increased. Compared with the model group, the GAS and PP of the positive control group rats were reduced. Compared with the model group, after gavage of the Chinese medicine preparations of low-dose Example 1 group, medium-dose Example 1 group, and high-dose Example 1 group, the GAS and PP of the rats were significantly reduced. And compared with the positive control group, the GAS and PP of the rats in the medium-dose Example 1 group and the high-dose Example 1 group of the present invention were higher than those in the positive control group. The above results show that the Chinese medicine preparation of the present invention is better than the drug in the positive control group in increasing gastric juice.

[0087] As shown in Table 2, compared with the normal control group, the LPO value of the model group rats increased after modeling, and the activities of SOD and GSH-Px decreased. The above results show that the gastric mucosa of the model group rats has undergone serious changes. Compared with the model group, after gavage of the Chinese medicine preparation of low-dose Example 1 group, medium-dose Example 1 group, and high-dose Example 1 group, the LPO value in the serum of the rats decreased, and the activities of SOD and GSH-Px increased. The above results show that the Chinese medicine preparation of the present invention can reduce the gastric mucosal lesions. As can be seen from the above, the Chinese medicine preparation of the present invention can improve the antioxidant capacity and reduce gastric mucosal damage, thereby effectively treating atrophic gastritis.

[0088] In the higher dose Example 1 group and the high dose Comparative Examples 1-4 groups, the gastric juice GAS and PP of rats were increased, the LPO value in the serum was significantly increased, and the activities of SOD and GSH-Px were significantly reduced. Compared with Example 1, the Chinese medicine preparation formula of Comparative Example 1 omitted the white flower bitter lantern root; the Chinese medicine preparation formula of Comparative Example 2 omitted the white flower bitter lantern root, and the amount of shrimp grass was increased; the Chinese medicine preparation formula of Comparative Example 3 omitted the shrimp grass; the Chinese medicine preparation formula of Comparative Example 4 omitted the shrimp grass, and the amount of white flower bitter lantern root was increased. It can be seen from the above that omitting any one of the components of white flower bitter lantern root and shrimp grass uniformly leads to a decrease in the efficacy of the Chinese medicine preparation. In summary, the Chinese medicine preparation of the present invention can effectively treat atrophic gastritis through the synergistic effect of the two main drugs of white flower bitter lantern root and shrimp grass.

[0089] In the high-dose Example 1 group and the high-dose Comparative Example 5 group, the gastric juice GAS and PP of rats increased slightly, the LPO value in serum increased, and the activities of SOD and GSH-Px decreased. Compared with Example 1, the cucumber seeds were omitted in the Chinese medicine preparation formula of Comparative Example 5. As can be seen from the above, omitting the cucumber seeds will affect the efficacy of the Chinese medicine preparation of the present invention.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A Chinese medicine preparation for treating chronic atrophic gastritis, characterized in that: The invention is composed of the following raw materials in parts by weight: 30-40 parts of Radix Achyranthis Bidentatae, 25-35 parts of Herba Schizonepetae, 10-20 parts of dried tangerine peels, 12-18 parts of immature bitter oranges, 6-10 parts of cucumber seeds, 8-10 parts of spiny jujube seeds, 2-5 parts of lilies and 1-3 parts of liquorice.

2. A Chinese medicine preparation for treating chronic atrophic gastritis, characterized in that: The invention is composed of the following raw materials in parts by weight: 37 parts of Radix Achyranthis Bidentatae, 29 parts of Herba Schizonepetae, 14 parts of dried tangerine peels, 13 parts of immature bitter oranges, 8 parts of cucumber seeds, 9 parts of spiny jujube seeds, 4 parts of lilies and 2 parts of liquorice.

3. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to any one of claims 1 or 2, characterized in that: The following steps are involved: 1) Weighing and mixing the raw materials according to the weight ratio, soaking, decocting and filtering to obtain a first decoction and a first filter residue; 2) adding water to the first filter residue of step 1), soaking, decocting and filtering to obtain a second decoction; combining the first decoction and the second decoction, concentrating to an extract with a relative density of 1.10-1.25, and preparing the extract with pharmaceutically acceptable excipients into a corresponding dosage form.

4. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to claim 3, characterized in that: The amount of water used in step 1) and step 2) is 10-15 times the total weight of the raw material.

5. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to claim 3, characterized in that: In the step 1), the soaking time is 1-2 hours, and the decocting time is 2-3 hours.

6. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to claim 3, characterized in that: In step 2), the soaking time is 2-3 hours, and the boiling time is 3-4 hours.

7. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to claim 3, characterized in that: The dosage form is an oral dosage form.

8. The method for preparing the Chinese medicine preparation for treating chronic atrophic gastritis according to claim 7, characterized in that: The oral dosage form is tablet, granule, capsule or oral liquid.

9. Use of the traditional Chinese medicine preparation for treating chronic atrophic gastritis according to any one of claims 1 or 2 in the preparation of a medicament for treating chronic atrophic gastritis.

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