Yeast rapid detection device and method

Abstract

The present invention relates to the technical field of yeast detection, and discloses a rapid yeast detection device and method, including a base plate, and also including: a tray, which is rotatably mounted on the top of the base plate; a sampling component, which is arranged above the base plate and is used to sample yeast fermentation products; a feeding component, which is used to feed the sampling component for sampling yeast fermentation products; and a detection component, which is used to detect yeast fermentation products. The present invention can continuously sample the yeast fermentation liquid inside the barrel body, and during sampling, the tray will drive the barrel body to rotate, so that the positions of continuous sampling are different, avoiding the occurrence of accidental sampling, and can also automatically replace the sampling tube after the sampling and detection are completed, avoiding the occurrence of cross contamination of continuous sampling, and multiple sampling and detection ensure the accuracy of the sampling and detection results, thereby improving the use effect of the device.

Description

Technical Field

[0001] The present invention relates to the technical field of yeast detection, and in particular to a rapid yeast detection device and method. Background Art

[0002] Yeast is a type of unicellular fungi belonging to the kingdom Fungi and the phylum Ascomycota. They are usually spherical or ellipsoidal in shape and reproduce by budding or fission. This tiny unicellular microorganism that is invisible to the naked eye can ferment sugar into alcohol and carbon dioxide. It is distributed throughout nature and is a typical heterotrophic facultative anaerobic microorganism that can survive both in aerobic and anaerobic conditions. It is a natural fermentation agent. During the cultivation of yeast, the test sample of yeast fermentation needs to be extracted.

[0003] For example, a yeast fermentation sample extraction device disclosed in the patent with announcement number CN219174474U can sample yeast, but it does not have the function of continuous sampling, and the sampling position is also fixed, which leads to accidental test results after sampling, reducing the effect of sampling test. Therefore, it is urgent to design a yeast rapid detection device. Summary of the invention

[0004] The purpose of the present invention is to solve the problems existing in the prior art and to propose a rapid yeast detection device and method.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A yeast rapid detection device comprises a substrate and further comprises:

[0007] A tray is rotatably mounted on the top of the base plate, a barrel is placed on the tray, and yeast fermentation products are arranged inside the barrel;

[0008] A sampling component is arranged above the base plate and is used for sampling yeast fermentation products;

[0009] A feeding component, which is arranged on the base plate and is used to feed the sampling component for sampling yeast fermentation products;

[0010] A detection component is arranged on the top of the base plate and is used to detect yeast fermentation products;

[0011] A driving component is arranged at the bottom of the base plate, and is used to drive the detection component, the tray and the feeding component.

[0012] As a further technical solution of the present invention, the sampling component includes: an L-shaped frame, the L-shaped frame is fixedly installed on the top of the base plate, a turntable is horizontally arranged below the horizontal side of the L-shaped frame, a first motor is installed on the top of the horizontal side of the L-shaped frame, the output shaft of the first motor is installed at the center of the top of the turntable, a rotating rod is installed at the bottom of the turntable for vertical rotation, and the end of the rotating rod is fixedly installed on the top of the base plate.

[0013] As a further technical solution of the present invention, four fixing frames evenly distributed in a ring shape are fixed to the bottom of the turntable, a sleeve is fixedly sleeved on each fixing frame, a sliding tube is slidably arranged inside each sleeve, the end of the sliding tube passes through the bottom of the sleeve, a sampling tube is arranged at the bottom of the sliding tube, and each sliding tube is provided with a fixing mechanism for fixing the sampling tube.

[0014] As a further technical solution of the present invention, a first round roller and a second round roller are fixedly sleeved on the surface of the rotating rod, the second round roller is located above the first round roller, a second folding groove is opened on the first round roller, and a first folding groove is opened on the second round roller. A sliding rod is rotatably installed at the end of the side surface of each sliding tube, and the end of each sliding rod away from the sliding tube is slidably installed inside the second folding groove, a piston disk is slidably arranged inside each sleeve, a folding rod is provided on the top of each sleeve, the end of each vertical side of the folding rod is fixedly installed on the top of the corresponding piston disk, and the end of each horizontal side of the folding rod is slidably installed inside the first folding groove, a second spring is installed on the piston disk and the top of the sleeve, and the second spring is sleeved on the vertical side surface of the folding rod.

[0015] As a further technical solution of the present invention, the fixing mechanism includes: a fixing box, which is fixedly installed at the end of the sliding tube surface, two symmetrically arranged clamping plates are slidably arranged below the fixing box, a groove is provided at the bottom of the clamping plates, two symmetrically arranged slide plates are slidably arranged inside the fixing box, an open groove is provided at the bottom of the fixing box that penetrates into the interior of the fixing box, protrusions are fixed at the bottom of the two slide plates, the two protrusions pass through the open grooves and are respectively fixedly installed on the tops of the two clamping plates, third spring rods are fixedly installed on the opposite sides of the fixing box, and the corresponding telescopic ends of the third spring rods are fixedly installed on the corresponding sides of the slide plates.

[0016] As a further technical solution of the present invention, a connecting block is provided inside the fixed box, and the connecting block is located between the two slides. Movable rods are rotatably installed on the opposite sides of the connecting block. The ends of the two movable rods away from the connecting block are rotatably installed on the opposite sides of the two slides, and an insertion rod is fixedly installed horizontally on the side of the connecting block. The end of the insertion rod passes through the fixed box, and a Z-shaped rod is fixedly installed on the bottom of the horizontal side of the L-shaped frame. A stopper is fixedly installed at the end of the Z-shaped rod. When the insertion rod and the stopper contact, the insertion rod slides between the stopper, and a collection box is also fixedly installed on the top of the base plate.

[0017] As a further technical solution of the present invention, the feeding assembly includes: a feeding box, which is horizontally fixedly installed on the top of the base plate, a push plate is slidably arranged inside the feeding box, a first spring rod is fixedly installed on the inner side of the feeding box, the telescopic end of the first spring rod is fixedly installed on the side of the push plate, a top column is vertically arranged at the bottom of the feeding box, the top of the top column passes through the bottom of the feeding box, a fourth spring rod is vertically fixedly installed on the top of the base plate, the telescopic end of the fourth spring rod is fixedly installed on the bottom of the top column, a third round roller is vertically rotatably installed on the top of the base plate, an oblique groove and a vertical groove are opened on the surface of the third round roller, the oblique groove and the vertical groove are connected end to end, a sliding column is fixedly and rotatably installed on the end of the surface of the top column, and the end of the sliding column away from the top column is slidably arranged inside the oblique groove and the vertical groove.

[0018] As a further technical solution of the present invention, the detection component includes: a carrying plate, which is horizontally arranged above the substrate, with a column vertically fixedly installed at the center of the bottom of the carrying plate, and the end of the column is rotatably installed on the top of the substrate, four material discharge sheets distributed in a ring are arranged on the top of the carrying plate, and a microscope is also fixedly installed on the top of the substrate.

[0019] As a further technical solution of the present invention, the driving component includes: a first belt, two first pulleys are rotatably installed at the bottom of the base plate, the first belt is sleeved on the surfaces of the two first pulleys, the two first pulleys are fixedly installed at the bottom center of the tray and the bottom center of the column respectively through a connecting shaft, a large pulley is fixedly installed at the bottom of the corresponding first pulley, a small pulley is also rotatably installed at the bottom of the base plate, the second belt is sleeved on the surfaces of the large pulley and the small pulley, the small pulley is fixedly installed at the bottom center of the third roller through a connecting shaft, a second motor is installed at the bottom of the base plate, and the output shaft of the second motor is installed at the bottom center of the corresponding first pulley.

[0020] A rapid yeast detection method comprises the following steps:

[0021] S1: First, place the barrel 11 filled with yeast fermentation liquid on the tray 16, and then start the first motor 3 and the second motor 15 synchronously. It should be noted that the first motor 3 and the second motor 15 are both intermittent motors. The first motor 3 drives the turntable 10 to rotate, the rotation of the turntable 10 drives the fixed frame 21 to rotate, the rotation of the fixed frame 21 drives the sleeve 18 to rotate, and the rotation of the sleeve 18 drives the slide tube 19, the fixing mechanism and the sampling tube 20 to rotate. Due to the arrangement of the first round roller 17, the slide rod 37 and the second folding groove 36, the slide tube 19 will move along the second folding groove 36 when rotating. At this time, the slide rod 37 will first move horizontally for a distance The sampling tube 20 and the top column 26 are separated, and the sampling tube 20 and the top column 26 are tightly pressed and unable to move. Then the sliding rod 37 moves downward again, and the sliding tube 19 is first rotated horizontally and then rotated downward, so that the sampling tube 20 can enter the barrel body 11. Due to the arrangement of the second round roller 31 and the first folding groove 35, the folding rod 33 drives the piston disc 32 to rotate horizontally first. When the sampling tube 20 gradually enters the yeast fermentation liquid, the folding rod 33 drives the piston disc 32 to move upward while rotating, and the second spring 34 generates elastic force. The upward movement of the piston disc 32 causes the fermentation liquid inside the barrel body 11 to be extracted into the sampling tube 20, and the sampling is completed.

[0022] S2: The operation of the second motor 15 drives the first belt 13, the first pulley, the second belt 14, the large pulley and the small pulley to rotate. The rotation of the first pulley and the small pulley drives the column, the tray 16 and the third roller 28 to rotate. It should be noted that when the large pulley rotates a quarter of a circle, the small pulley rotates one circle. The rotation of the column drives the carrier plate 4 to rotate. The rotation of the tray 16 drives the barrel 11 to rotate. It has been realized that the sampling tube 20 can sample yeast liquid at different positions each time. The rotation of the carrier plate 4 can make the yeast liquid sampled each time drip onto different discharge sheets. The rotation of the small pulley drives the third roller 28 to rotate. At this time, the rotation of the third roller 28 drives the sliding column to move downward, and the movement of the sliding column drives the top column 26 to move downward.

[0023] S3: When the first motor 3 and the second motor 15 work for the second time, the sampling tube 20 after sampling will move along the second folding groove 36 as a path, and the sampling tube 20 will move upward. At this time, the folding rod 33 will rotate horizontally along the first folding groove 35 as a path. When the first motor 3 and the second motor 15 work for the third time, the sampling tube 20 will rotate horizontally along the second folding groove 36 as a path to above the discharge sheet. When the first motor 3 finishes working for the third time, the elastic force generated by the second spring 34 will cause the piston disc 32 and the folding rod 33 to move downward. The downward movement of the piston disc 32 will cause the yeast liquid inside the sampling tube 20 to be discharged and dripped onto the corresponding discharge sheet for observation by the microscope 5. When the first motor 3 and the second motor 15 work for the fourth time, the sampling tube 20 will rotate horizontally along the second folding groove 36 as a path to the starting position, and the folding rod 33 will also rotate horizontally along the first folding groove 35 to the starting position. During this process, the insertion rod 23 will contact the stopper 7 and cause the insertion rod 23 to move into the fixed box 22. The movement of the insertion rod 23 drives the moving block The movement of the moving block drives the movable rod 40 and the slide plate 38 to move. The movement of the slide plate 38 drives the clamping plates 42 to move away from each other and causes the third spring rod 41 to generate elastic force. Then, the sampling tube 20 located between the two clamping plates 42 will fall into the collecting box 6. When the slide tube 19 without the sampling tube 20 installed rotates to the top of the feeding box 9, at this time, the third round roller 28 rotates one circle, so that the slide column is located below the vertical groove 30 and at the connection between the inclined groove 29. Then, the elastic force generated by the fourth spring rod 27 will cause the top column 26 to move. The top column 26 moves upward, and the unused sampling tubes 20 located in the same plane move upward, so that the unused sampling tubes 20 can contact the groove at the bottom of the clamping plate 42 to open the clamping plate 42, and the clamping plate 42 can clamp the sampling tube 20. It should be noted that multiple sampling tubes 20 are placed on the side of the push plate 24, so that there will always be unused sampling tubes 20 pushed to the top of the top column 26, so as to realize the removal and replacement of the sampling tubes 20, and avoid cross contamination during sampling and detection.

[0024] The beneficial effects of the present invention are:

[0025] Firstly, the present invention can continuously sample the yeast fermentation liquid in the barrel body through the arrangement of the sampling component and the feeding component, and the tray will drive the barrel body to rotate during sampling, so that the positions of continuous sampling are different, thereby avoiding the occurrence of accidental sampling, and the sampling tube can be automatically replaced after the sampling and detection are completed, thereby avoiding the occurrence of cross contamination in continuous sampling, and multiple sampling and detection ensures the accuracy of the sampling and detection results, thereby improving the use effect of the device;

[0026] Secondly, the present invention, through the arrangement of the detection component, the feeding component and the driving component, can first dismantle the used sampling tubes and then install the unused sampling tubes after the sampling and testing is completed, so as to avoid cross-contamination interference during the sampling and testing, thereby improving the use effect of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the structure of a rapid yeast detection device proposed by the present invention;

[0028] Figure 2 for Figure 1 A magnified schematic diagram of part A;

[0029] Figure 3 This is a schematic diagram of the structure of a rapid yeast detection device proposed by the present invention from a bottom-up perspective;

[0030] Figure 4 This is a rear structural schematic diagram of a rapid yeast detection device proposed by the present invention;

[0031] Figure 5 This is a schematic cross-sectional view of a feed box of a yeast rapid detection device proposed by the present invention;

[0032] Figure 6 This is a schematic structural diagram of the first round roller, the second round roller and the third round roller of a yeast rapid detection device proposed by the present invention;

[0033] Figure 7 This is a schematic diagram of the cross-sectional structure of a sleeve of a rapid yeast detection device proposed by the present invention;

[0034] Figure 8 for Figure 7 A magnified schematic diagram of part B;

[0035] Fig. 9 This is a schematic diagram of the structure of a slide tube and a fixed box of a rapid yeast detection device proposed by the present invention;

[0036] Fig.10 for Fig. 9 Enlarged schematic diagram of part C.

[0037] In the figure: 1, substrate; 2, L-shaped frame; 3, first motor; 4, carrier plate; 5, microscope; 6, collection box; 7, stopper; 8, Z-shaped rod; 9, feed box; 10, turntable; 11, barrel; 12, turnbar; 13, first belt; 14, second belt; 15, second motor; 16, tray; 17, first round roller; 18, sleeve; 19, slide tube; 20, sampling tube; 21, fixed frame; 22, fixed Box; 23, plug rod; 24, push plate; 25, first spring rod; 26, top column; 27, fourth spring rod; 28, third round roller; 29, inclined groove; 30, vertical groove; 31, second round roller; 32, piston plate; 33, folding rod; 34, second spring; 35, first folding groove; 36, second folding groove; 37, sliding rod; 38, sliding plate; 39, connecting block; 40, movable rod; 41, third spring rod; 42, clamping plate. DETAILED DESCRIPTION

[0038] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Please refer to the attached Figure 1 -Attached Fig.10 A yeast rapid detection device comprises a substrate 1, and also comprises: a tray 16, a sampling component, a feeding component, a detection component and a driving component, wherein the tray 16 is rotatably mounted on the top of the substrate 1, a barrel 11 is placed on the tray 16, and yeast fermentation is arranged inside the barrel 11, the sampling component is arranged above the substrate 1, the sampling component is used to sample the yeast fermentation, the feeding component is arranged on the substrate 1, the feeding component is used to feed the sampling component for sampling the yeast fermentation, the detection component is arranged on the top of the substrate 1, the detection component is used to detect the yeast fermentation, the driving component is arranged at the bottom of the substrate 1, and the driving component is used to drive the detection component, the tray 16 and the feeding component;

[0041] As described above, the yeast liquid can be sampled multiple times from different positions inside the barrel body 11 continuously, thereby ensuring the accuracy of sampling and detection.

[0042] Please refer to the attached Figure 1 -Attached Fig.10In a preferred embodiment, the sampling assembly includes: an L-shaped frame 2, the L-shaped frame 2 is fixedly installed on the top of the base plate 1, a turntable 10 is horizontally arranged below the horizontal side of the L-shaped frame 2, a first motor 3 is installed on the top of the horizontal side of the L-shaped frame 2, the output shaft of the first motor 3 is installed at the center of the top of the turntable 10, a rotating rod 12 is installed at the bottom of the turntable 10 for vertical rotation, the end of the rotating rod 12 is fixedly installed on the top of the base plate 1, four annularly evenly distributed fixed frames 21 are fixed at the bottom of the turntable 10, each fixed frame 21 is fixedly sleeved with a sleeve 18, each sleeve 18 is slidably arranged with a slide tube 19, the end of the slide tube 19 passes through the bottom of the sleeve 18, a sampling tube 20 is arranged at the bottom of the slide tube 19, each slide tube 19 is provided with a fixing mechanism for fixing the sampling tube 20, the rotating rod 1 2 surface is fixedly sleeved with a first round roller 17 and a second round roller 31, the second round roller 31 is located above the first round roller 17, a second folding groove 36 is opened on the first round roller 17, and a first folding groove 35 is opened on the second round roller 31, a sliding rod 37 is rotatably installed at the end of the side of each sliding tube 19, and the end of each sliding rod 37 away from the sliding tube 19 is slidably installed in the second folding groove 36, a piston disc 32 is slidably arranged inside each sleeve 18, a folding rod 33 is provided at the top of each sleeve 18, the end of each vertical side of the folding rod 33 is fixedly installed on the top of the corresponding piston disc 32, and the end of each horizontal side of the folding rod 33 is slidably installed in the first folding groove 35, a second spring 34 is installed at the top of the piston disc 32 and the sleeve 18, and the second spring 34 is sleeved on the vertical side surface of the folding rod 33;

[0043] Preferably, the folding rod 33 drives the piston disc 32 to rotate and move upward, and the second spring 34 generates elastic force, and the upward movement of the piston disc 32 causes the fermentation liquid inside the barrel body 11 to be extracted into the sampling tube 20, thereby completing the sampling.

[0044] Please refer to the attached Figure 5 -Attached Fig.10In a preferred embodiment, the fixing mechanism includes: a fixing box 22, the fixing box 22 is fixedly installed at the end of the surface of the sliding tube 19, two symmetrically arranged clamping plates 42 are slidably arranged below the fixing box 22, the bottom of the clamping plates 42 is provided with a groove, two symmetrically arranged slide plates 38 are slidably arranged inside the fixing box 22, the bottom of the fixing box 22 is provided with an open groove that penetrates into the inside of the fixing box 22, the bottoms of the two slide plates 38 are fixed with protrusions, the two protrusions pass through the open grooves and are respectively fixedly installed on the tops of the two clamping plates 42, and the opposite sides inside the fixing box 22 are fixedly installed with third spring rods 41, and the corresponding telescopic ends of the third spring rods 41 It is fixedly installed on the corresponding side of the slide 38, and a connecting block 39 is provided inside the fixing box 22. The connecting block 39 is located between the two slides 38. The opposite sides of the connecting block 39 are rotatably installed with movable rods 40. The ends of the two movable rods 40 away from the connecting block 39 are rotatably installed on the opposite sides of the two slides 38. The plug rod 23 is fixedly installed horizontally on the side of the connecting block 39. The end of the plug rod 23 passes through the fixing box 22. A Z-shaped rod 8 is fixedly installed at the bottom of the horizontal side of the L-shaped frame 2. A stopper 7 is fixedly installed at the end of the Z-shaped rod 8. When the plug rod 23 and the stopper 7 are in contact, the plug rod 23 slides between the stopper 7. A collecting box 6 is also fixedly installed on the top of the base plate 1.

[0045] Furthermore, the slide plate 38 moves to drive the clamping plates 42 away from each other and causes the third spring rod 41 to generate elastic force (no longer clamping the sampling tube 20 ), and the sampling tube 20 located between the two clamping plates 42 will fall into the collecting box 6 .

[0046] Please refer to the attached Figure 2 -Attached Fig. 9 In a preferred embodiment, the feeding assembly includes: a feeding box 9, the feeding box 9 is horizontally fixedly installed on the top of the base plate 1, a push plate 24 is slidably arranged inside the feeding box 9, a first spring rod 25 is fixedly installed on the side of the inner side of the feeding box 9, and the telescopic end of the first spring rod 25 is fixedly installed on the side of the push plate 24, a top column 26 is vertically arranged at the bottom of the feeding box 9, and the top of the top column 26 passes through the bottom of the feeding box 9, a fourth spring rod 27 is vertically fixedly installed on the top of the base plate 1, and the telescopic end of the fourth spring rod 27 is fixedly installed on the bottom of the top column 26, and a third round roller 28 is vertically rotatably installed on the top of the base plate 1, and an inclined groove 29 and a vertical groove 30 are opened on the surface of the third round roller 28, and the inclined groove 29 and the vertical groove 30 are connected end to end, and a sliding column is fixedly rotatably installed on the end of the surface of the top column 26, and the end of the sliding column away from the top column 26 is slidably arranged inside the inclined groove 29 and the vertical groove 30;

[0047] Specifically, the top column 26 moves upward to drive the unused sampling tubes 20 located in the same plane to move upward, so that the unused sampling tubes 20 can contact the groove at the bottom of the clamping plate 42 to open the clamping plate 42 and clamp the sampling tubes 20.

[0048] Please refer to the attached Figure 1 -Attached Figure 4 In a preferred embodiment, the detection assembly includes: a carrier plate 4, the carrier plate 4 is horizontally arranged above the substrate 1, a column is vertically fixedly installed at the center of the bottom of the carrier plate 4, the end of the column is rotatably installed on the top of the substrate 1, four material discharge sheets distributed in an annular manner are arranged on the top of the carrier plate 4, and a microscope 5 is also fixedly installed on the top of the substrate 1;

[0049] Furthermore, the downward movement of the piston plate 32 causes the yeast liquid in the sampling tube 20 to be discharged and dripped onto the corresponding material discharge sheet for observation by the microscope 5 .

[0050] Please refer to the attached Figure 1 -Attached Figure 6 In a preferred embodiment, the driving assembly includes: a first belt 13, two first pulleys are rotatably installed at the bottom of the base plate 1, the first belt 13 is sleeved on the surfaces of the two first pulleys, the two first pulleys are respectively fixedly installed at the bottom center of the tray 16 and the bottom center of the column through a connecting shaft, a large pulley is fixedly installed at the bottom of the corresponding first pulley, a small pulley is also rotatably installed at the bottom of the base plate 1, the large pulley and the small pulley surface are sleeved with a second belt 14, the small pulley is fixedly installed at the bottom center of the third roller 28 through a connecting shaft, a second motor 15 is installed at the bottom of the base plate 1, and the output shaft of the second motor 15 is installed at the bottom center of the corresponding first pulley;

[0051] The above is used to drive the first belt 13, the first pulley, the second belt 14, the large pulley and the small pulley to rotate. The rotation of the first pulley and the small pulley drives the column, the tray 16 and the third roller 28 to rotate.

[0052] Working principle of the present invention:

[0053] First, place the barrel 11 filled with yeast fermentation liquid on the tray 16, and then start the first motor 3 and the second motor 15 synchronously. It should be noted that the first motor 3 and the second motor 15 are both intermittent motors. The first motor 3 drives the turntable 10 to rotate, the rotation of the turntable 10 drives the fixed frame 21 to rotate, the rotation of the fixed frame 21 drives the sleeve 18 to rotate, and the rotation of the sleeve 18 drives the slide tube 19, the fixing mechanism and the sampling tube 20 to rotate. Due to the arrangement of the first round roller 17, the slide rod 37 and the second folding groove 36, the slide tube 19 will move along the second folding groove 36 as a path when rotating {taking the sampling tube 20 located above the feed box 9 as an example for description}, at this time, the slide rod 3 7 will first move horizontally for a distance, and the sampling tube 20 and the top column 26 will be tightly pressed against each other and unable to move. Then the sliding rod 37 will move downward again, so that the sliding tube 19 will first rotate horizontally and then rotate downward, so that the sampling tube 20 can enter the barrel body 11. Due to the arrangement of the second round roller 31 and the first folding groove 35, the folding rod 33 will drive the piston disc 32 to rotate horizontally first. When the sampling tube 20 gradually enters the yeast fermentation liquid, the folding rod 33 will drive the piston disc 32 to move upward while rotating, and the second spring 34 will generate elastic force. The upward movement of the piston disc 32 will cause the fermentation liquid inside the barrel body 11 to be extracted into the sampling tube 20, and the sampling is completed.

[0054] The operation of the second motor 15 will drive the first belt 13, the first pulley, the second belt 14, the large pulley and the small pulley to rotate. The rotation of the first pulley and the small pulley drives the column, the tray 16 and the third roller 28 to rotate. It should be noted that when the large pulley rotates a quarter of a circle, the small pulley rotates one circle. The rotation of the column drives the carrier plate 4 to rotate. The rotation of the tray 16 drives the barrel 11 to rotate. It has been realized that the sampling tube 20 can sample yeast liquid at different positions each time, and the rotation of the carrier plate 4 can make the yeast liquid sampled each time drip onto different discharge sheets. The rotation of the small pulley drives the third roller 28 to rotate. At this time, the rotation of the third roller 28 drives the sliding column to move downward, and the movement of the sliding column drives the top column 26 to move downward.

[0055] When the first motor 3 and the second motor 15 work for the second time, the sampling tube 20 after sampling will move along the second folding groove 36 as a path, and the sampling tube 20 will move upwards. At this time, the folding rod 33 will rotate horizontally along the first folding groove 35 as a path;

[0056] When the first motor 3 and the second motor 15 work for the third time, the sampling tube 20 will rotate horizontally to the top of the material discharge sheet along the second folding groove 36. When the first motor 3 finishes working for the third time, the elastic force generated by the second spring 34 will cause the piston disc 32 and the folding rod 33 to move downward. The downward movement of the piston disc 32 will cause the yeast liquid in the sampling tube 20 to be discharged and dripped onto the corresponding material discharge sheet for observation by the microscope 5.

[0057] When the first motor 3 and the second motor 15 work for the fourth time, the sampling tube 20 will rotate horizontally to the starting position along the second folding groove 36 as the path, and the folding rod 33 will also rotate horizontally to the starting position along the first folding groove 35. During this process, the insertion rod 23 will contact the stopper 7 and move the insertion rod 23 into the fixed box 22. The movement of the insertion rod 23 drives the moving block to move, and the movement of the moving block drives the movable rod 40 and the slide plate 38 to move. The movement of the slide plate 38 drives the clamping plates 42 to move away from each other and causes the third spring rod 41 to generate elastic force, so that the sampling tube 20 located between the two clamping plates 42 will fall into the collecting box 6. When the slide tube 19 without the sampling tube 20 installed rotates to the top of the feeding box 9, At this time, the third round roller 28 rotates one circle, so that the sliding column is located at the connection between the lower part of the vertical groove 30 and the inclined groove 29. Then, the elastic force generated by the fourth spring rod 27 will cause the top column 26 to move upward. The upward movement of the top column 26 drives the unused sampling tubes 20 located in the same plane to move upward, so that the unused sampling tubes 20 will contact the groove at the bottom of the clamping plate 42 to open the clamping plate 42, and the clamping plate 42 clamps the sampling tube 20. It should be noted that multiple sampling tubes 20 are placed on the side of the push plate 24, so that there will always be unused sampling tubes 20 pushed to the top of the top column 26, so as to realize the disassembly and replacement of the sampling tubes 20, and avoid cross contamination during sampling and detection.

[0058] In summary, by reciprocating in sequence, the yeast liquid can be sampled multiple times from different positions inside the barrel body 11 continuously, thereby ensuring the accuracy of sampling and detection, and further improving the use effect of the equipment.

[0059] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A yeast rapid detection device, comprising a substrate (1), characterized in that: Also includes: A tray (16), the tray (16) being rotatably mounted on the top of the base plate (1), a barrel (11) being placed on the tray (16), and yeast fermentation products being arranged inside the barrel (11); A sampling component, the sampling component is arranged above the base plate (1), and the sampling component is used to sample yeast fermentation products; A feeding component, the feeding component is arranged on the base plate (1), and the feeding component is used to feed a sampling component for sampling yeast fermentation products; A detection component, the detection component is arranged on the top of the base plate (1), and the detection component is used to detect yeast fermentation products; A driving component, the driving component being arranged at the bottom of the base plate (1), and being used to drive the detection component, the tray (16) and the feeding component; The sampling assembly comprises: an L-shaped frame (2), the L-shaped frame (2) being fixedly mounted on the top of a base plate (1), a turntable (10) being horizontally arranged below a horizontal side of the L-shaped frame (2), a first motor (3) being mounted on the top of the horizontal side of the L-shaped frame (2), an output shaft of the first motor (3) being mounted at the top center of the turntable (10), a rotating rod (12) being mounted vertically at the bottom of the turntable (10), a distal end of the rotating rod (12) being fixedly mounted on the top of the base plate (1), Four annularly evenly distributed fixing frames (21) are fixed to the bottom of the rotating disk (10); a sleeve (18) is fixedly sleeved on each fixing frame (21); a sliding tube (19) is slidably arranged inside each sleeve (18); the end of the sliding tube (19) passes through the bottom of the sleeve (18); a sampling tube (20) is arranged at the bottom of the sliding tube (19); each sliding tube (19) is provided with a fixing mechanism for fixing the sampling tube (20); and the surface of the rotating rod (12) A first round roller (17) and a second round roller (31) are fixedly sleeved, the second round roller (31) is located above the first round roller (17), the first round roller (17) is provided with a second folding groove (36), the second round roller (31) is provided with a first folding groove (35), a sliding rod (37) is rotatably mounted on the side end of each slide tube (19), the end of each slide rod (37) away from the slide tube (19) is slidably mounted inside the second folding groove (36), and each sleeve A piston disc (32) is slidably arranged inside the tube (18), a folding rod (33) is arranged on the top of each sleeve (18), the end of the vertical side of each folding rod (33) is fixedly installed on the top of the corresponding piston disc (32), and the end of the horizontal side of each folding rod (33) is slidably installed inside the first folding groove (35), and a second spring (34) is installed on the top of the piston disc (32) and the sleeve (18), and the second spring (34) is sleeved on the vertical side surface of the folding rod (33); The material feeding assembly comprises: a material feeding box (9), the material feeding box (9) being fixedly mounted horizontally on the top of the base plate (1), a push plate (24) being slidably mounted inside the material feeding box (9), a first spring rod (25) being fixedly mounted on the side of the inside of the material feeding box (9), a telescopic end of the first spring rod (25) being fixedly mounted on the side of the push plate (24), a top column (26) being vertically mounted on the bottom of the material feeding box (9), the top of the top column (26) passing through the bottom of the material feeding box (9), and the top of the base plate (1) being vertically fixed A fourth spring rod (27) is installed, the telescopic end of the fourth spring rod (27) is fixedly installed at the bottom of the top column (26), a third round roller (28) is vertically rotatably installed on the top of the base plate (1), an inclined groove (29) and a vertical groove (30) are opened on the surface of the third round roller (28), the inclined groove (29) and the vertical groove (30) are connected end to end, a sliding column is fixedly rotatably installed on the end of the surface of the top column (26), and the end of the sliding column away from the top column (26) is slidably arranged inside the inclined groove (29) and the vertical groove (30); The detection component comprises: a carrier plate (4), the carrier plate (4) being horizontally arranged above the base plate (1), a column being vertically fixedly installed at the center of the bottom of the carrier plate (4), the end of the column being rotatably installed on the top of the base plate (1), four material discharge sheets being arranged in an annular pattern on the top of the carrier plate (4), a microscope (5) being also fixedly installed on the top of the base plate (1), and the driving component comprising: a first belt (13), two first pulleys being rotatably installed at the bottom of the base plate (1), the first belt (13) being sleeved on the two first pulleys. The two first pulleys are respectively fixedly mounted at the bottom center of the tray (16) and the bottom center of the column through a connecting shaft, a large pulley is fixedly mounted at the bottom of the corresponding first pulley, a small pulley is rotatably mounted at the bottom of the base plate (1), a second belt (14) is sleeved on the surface of the large pulley and the small pulley, the small pulley is fixedly mounted at the bottom center of the third roller (28) through a connecting shaft, a second motor (15) is mounted at the bottom of the base plate (1), and the output shaft of the second motor (15) is mounted at the bottom center of the corresponding first pulley.

2. A rapid yeast detection device according to claim 1, characterized in that: The fixing mechanism comprises: a fixing box (22), the fixing box (22) being fixedly mounted on the end of the surface of the sliding tube (19), two symmetrically arranged clamping plates (42) being slidably mounted below the fixing box (22), the bottom of the clamping plates (42) being provided with a groove, two symmetrically arranged slide plates (38) being slidably mounted inside the fixing box (22), the bottom of the fixing box (22) being provided with an open groove penetrating into the inside of the fixing box (22), the bottoms of the two slide plates (38) being fixed with protrusions, the two protrusions being passed through the open grooves and being respectively fixedly mounted on the tops of the two clamping plates (42), and third spring rods (41) being fixedly mounted on opposite sides inside the fixing box (22), the telescopic ends of the corresponding third spring rods (41) being fixedly mounted on the corresponding sides of the slide plates (38).

3. A rapid yeast detection device according to claim 2, characterized in that: A connecting block (39) is provided inside the fixing box (22), and the connecting block (39) is located between the two slides (38). The opposite sides of the connecting block (39) are rotatably mounted with movable rods (40), and the ends of the two movable rods (40) away from the connecting block (39) are rotatably mounted on the opposite sides of the two slides (38). An insertion rod (23) is horizontally fixedly mounted on the side of the connecting block (39), and the end of the insertion rod (23) passes through the fixing box (22). A Z-shaped rod (8) is fixedly mounted on the bottom of the horizontal side of the L-shaped frame (2), and a stopper (7) is fixedly mounted on the end of the Z-shaped rod (8). When the insertion rod (23) and the stopper (7) are in contact, the insertion rod (23) and the stopper (7) slide therebetween. A collecting box (6) is also fixedly mounted on the top of the base plate (1).

4. A rapid yeast detection method, characterized in that: The yeast rapid detection device according to any one of claims 1 to 3 comprises the following steps: S1: First, the barrel (11) containing the yeast fermentation liquid is placed on the tray (16), and then the first motor (3) and the second motor (15) are started synchronously. It should be noted that the first motor (3) and the second motor (15) are both intermittent motors. The first motor (3) drives the turntable (10) to rotate, the turntable (10) drives the fixed frame (21) to rotate, the fixed frame (21) drives the sleeve (18) to rotate, and the sleeve (18) drives the slide tube (19), the fixing mechanism and the sampling tube (20) to rotate. Due to the arrangement of the first round roller (17), the slide rod (37) and the second folding groove (36), the slide tube (19) will move along the second folding groove (36) as a path when rotating. At this time, the slide rod (37) will first move horizontally. After the sample tube (20) and the top column (26) are moved for a certain distance, the sample tube (20) and the top column (26) are pressed against each other and cannot move. Then, the slide bar (37) moves downward again, so that the slide tube (19) first rotates horizontally and then rotates downward, so that the sample tube (20) can enter the barrel body (11). Due to the arrangement of the second round roller (31) and the first folding groove (35), the folding bar (33) drives the piston plate (32) to rotate horizontally first. When the sample tube (20) gradually enters the yeast fermentation liquid, the folding bar (33) drives the piston plate (32) to rotate and move upward, and the second spring (34) generates elastic force. The upward movement of the piston plate (32) causes the fermentation liquid inside the barrel body (11) to be drawn into the sample tube (20), thereby completing the sampling. S2: The operation of the second motor (15) drives the first belt (13), the first pulley, the second belt (14), the large pulley and the small pulley to rotate. The rotation of the first pulley and the small pulley drives the column, the tray (16) and the third roller (28) to rotate. It should be noted that when the large pulley rotates a quarter of a turn, the small pulley rotates one turn. The rotation of the column drives the carrier plate (4) to rotate. The rotation of the tray (16) drives the barrel (11) to rotate. This enables the sampling tube (20) to sample yeast liquid at different positions each time. The rotation of the carrier plate (4) enables the yeast liquid sampled each time to be dripped onto different discharge sheets. The rotation of the small pulley drives the third roller (28) to rotate. At this time, the rotation of the third roller (28) drives the sliding column to move downward. The movement of the sliding column drives the top column (26) to move downward. S3: When the first motor (3) and the second motor (15) work for the second time, the sampling tube (20) after sampling will move along the second folding groove (36) as a path, and the sampling tube (20) will move upward. At this time, the folding rod (33) will rotate horizontally along the first folding groove (35) as a path. When the first motor (3) and the second motor (15) work for the third time, the sampling tube (20) will rotate horizontally along the second folding groove (36) as a path to above the discharge sheet. When the first motor (3) finishes working for the third time, the elastic force generated by the second spring (34) causes the piston plate (32) and the folding rod ( When the first motor (3) and the second motor (15) are operated for the fourth time, the sampling tube (20) will rotate horizontally to the starting position along the second folding groove (36), and the folding rod (33) will also rotate horizontally to the starting position along the first folding groove (35). During this process, the insertion rod (23) will contact the stopper (7) and move the insertion rod (23) into the fixed box (22). The movement of the insertion rod (23) drives the moving The block moves, and the movement of the moving block drives the movable rod (40) and the slide plate (38) to move. The slide plate (38) moves and drives the clamping plates (42) to move away from each other and causes the third spring rod (41) to generate elastic force. Then, the sampling tube (20) located between the two clamping plates (42) will fall into the inside of the collection box (6). When the slide tube (19) without the sampling tube (20) installed rotates to the top of the feed box (9), at this time, the third round roller (28) rotates one circle, so that the slide column is located below the vertical groove (30) and at the connection between the inclined groove (29). Then, the elastic force generated by the fourth spring rod (27) causes the top column ( 26) moves upward, and the top column (26) moves upward, driving the unused sampling tubes (20) located in the same plane to move upward, so that the unused sampling tubes (20) can contact the groove at the bottom of the clamping plate (42) to open the clamping plate (42), and the clamping plate (42) can clamp the sampling tube (20). It should be noted that multiple sampling tubes (20) are placed on the side of the push plate (24), so that there will always be unused sampling tubes (20) pushed to the top of the top column (26), so as to realize the removal and replacement of the sampling tubes (20), thereby avoiding cross contamination during sampling and testing.

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