Artificial fertilization method for eel eggs

Abstract

The present invention discloses a method for artificially inseminating eel eggs, comprising the following steps: 1. preparing eel semen and female eel eggs; 2. preparing an eel egg artificial insemination device and an egg incubation device; 3. adding eel semen and semen activation liquid to the artificial insemination device; 4. placing eggs in an egg receiving tray and placing it on a tray rack within the device, activating the device, extracting and atomizing eel semen so that it adheres to the surface of the eel eggs; 5. activating an activation unit and spraying activation liquid into the egg receiving tray to activate the eel sperm; 6. placing the fertilized eel eggs in the egg incubation device for incubation. The artificial fertilization method for eel eggs recorded in the present invention greatly shortens the migration distance of eel sperm after activation, allows the sperm to combine with the egg in a short time to complete fertilization, and the whole process does not touch the eel eggs, thereby greatly improving the fertilization success rate of the eggs.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural product breeding, and in particular to a method for artificial fertilization of eel eggs. Background Art

[0002] Artificial breeding of eels is mainly divided into paddy field ecological breeding and indoor fully artificial breeding.

[0003] Among them, in the indoor fully artificial breeding technology, the eel egg fertilization methods are mainly divided into wet fertilization and dry fertilization. The wet fertilization is to pour the taken out fish eggs and sperm into the water at the same time, and the dry fertilization is to mix the fish eggs and sperm first and then pour them into the water.

[0004] Yellow eel is a fish species with relatively small egg-laying capacity. Each female carries between dozens and a thousand eggs. Dry fertilization takes a long time to operate, and the eggs will be damaged during mixing and stirring, which will affect the fertilization rate. Therefore, the wet fertilization operation is basically adopted in yellow eel farming.

[0005] However, wet fertilization actually simulates the process of natural fertilization in a small space. However, since the survival time of eel sperm after being activated by water is usually less than five minutes, the optimal fertilization time is 1-2 minutes. In addition, eel eggs are large, sperm swims slowly, and an egg has only one fertilization hole. After the egg encounters water, it quickly absorbs water. After it is full of water, the fertilization hole is closed, and the sperm cannot enter normally for fertilization. If the problem of low fertilization efficiency is solved by increasing the density of sperm, the demand for male broodstock will increase greatly, which is not conducive to the large-scale breeding of fully artificial seedlings.

[0006] Based on the above-mentioned difficulties in the artificial breeding technology of rice field eels, we propose a method for artificial fertilization of rice field eel eggs. Summary of the Invention

[0007] The purpose of the present invention is to provide an artificial fertilization device and a fertilization method for yellow eel eggs, so as to solve the above problems existing in the prior art.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] A method for artificial fertilization of yellow eel eggs, comprising:

[0010] Step 1: Prepare male and female broodstock, extract eel semen and female eel eggs, and place the extracted eel semen and eggs in sterile containers for later use.

[0011] Step 2: Prepare the artificial fertilization device for eel eggs and the fish egg hatching equipment. In order to provide a relatively sterile and safe environment for the fertilization of eel eggs, it is necessary to implement all-round sterile disinfection inside and outside the equipment. The disinfection of the equipment can be selected but is not limited to ozone disinfection.

[0012] The artificial fertilization device for yellow eel eggs comprises a base, a nozzle driving assembly, an egg tray transmission assembly and an activation unit.

[0013] Furthermore, the nozzle drive assembly is installed on the top of the base, and the nozzle drive assembly includes a vertical frame fixedly installed with the base, and a No. 2 screw transmission mechanism is horizontally installed on the vertical frame, and a carrier plate is installed at the bottom of the screw slider of the No. 2 screw transmission mechanism, and an atomizing device is installed at the end of the carrier plate. The liquid inlet port of the atomizing device is connected to the containing tank through a pipe, wherein the main function of the nozzle drive assembly is to drive the atomizing device to move back and forth in a horizontal direction above the egg tray transmission assembly, and cooperate with the start-up of the atomizing device to ensure that sperm is evenly attached to the egg membrane on the surface of the eel eggs.

[0014] Furthermore, the egg tray transmission assembly is arranged at the bottom of the nozzle drive assembly, and the egg tray transmission assembly also includes a machine base, a No. 1 screw transmission mechanism, a tray rack and an egg holding tray for holding eel eggs. The egg holding tray is placed on the tray rack, and the sizes and structures between the tray rack and the egg holding tray are adapted to each other. The tray rack is horizontally installed on the screw slider assembly of the No. 1 screw transmission mechanism; the activation unit includes an activation liquid storage tank, a delivery pump and a spray pipe; the liquid inlet end of the delivery pump is connected to the activation liquid storage tank by a pipeline, and the liquid outlet of the delivery pump is connected to the spray pipe.

[0015] Step 3: Add the eel semen and semen activation liquid into the corresponding holding tank and activation liquid storage box in the artificial insemination equipment prepared in step 2, respectively, in preparation for the eel egg fertilization operation. Before adding the eel semen into the holding tank, sperm preservation liquid needs to be added to dilute the eel sperm. The diluted eel sperm concentration is 900,000 to 1.1 million sperm / ml.

[0016] Step 4: The fish eggs obtained in step 1 are contained in an egg holding tray, and the egg holding tray is placed on a tray rack in the equipment, the equipment is started, and No. 1 screw transmission mechanism in the egg tray transmission assembly is operated, and the tray rack and the egg holding tray thereon are moved to the nozzle drive assembly position; the atomizing device in the equipment is started to extract and atomize the eel semen inside the holding tank so that it adheres to the surface of the eel eggs placed in the egg holding tray.

[0017] Step 5. After all the eel eggs placed in the egg holding tray have completed the atomization attachment of the eel semen, the activation unit in the equipment is started, and the activation liquid in the activation liquid storage tank is transported to the spray pipe, and sprayed into the egg holding tray to activate the eel sperm attached to the surface of the eel eggs and fertilize the eel eggs. When the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius and the fertilization time is 1-2 minutes.

[0018] Step 6: After the fertilization of the yellow eel eggs is completed, they can be placed in the fish egg incubation equipment prepared in step 2 to start hatching. After completing the fertilization operation of all the yellow eel eggs, the equipment is fully cleaned and disinfected again and sealed up for the next fertilization operation.

[0019] Furthermore, the atomizing device in step 2 is composed of a pump and an atomizing nozzle, wherein the atomizing nozzle is installed at the outlet end of the pump, the pipeline is a disposable sterile tube, and the two ends of the pipeline are connected to the container and the atomizing device by quick-release connectors.

[0020] Furthermore, a splash-proof panel is installed on the periphery of the base of the egg tray transmission assembly in step 2, and the bottom of the egg tray is designed to be a square flat bottom structure, with both ends of the egg tray sliding against the inner side of the splash-proof panel.

[0021] Furthermore, the activation liquid storage tank and the delivery pump in step 2 are both installed on one side of the top of the base, and the spray pipe is installed horizontally at the front end of the stand in the nozzle drive assembly.

[0022] Furthermore, a protective shell cover is installed on the top of the base in step 2, which wraps the nozzle drive assembly, egg tray transmission assembly and activation unit. An egg tray access port is opened at one end of the protective shell cover. The egg tray access port is mainly for the convenience of the operator to take and place the egg tray.

[0023] Beneficial effects:

[0024] The artificial insemination method for yellow eel eggs described in the present invention comprises the following steps: uniformly attaching yellow eel sperm to the surface of the egg membrane of yellow eel eggs in an atomized manner; and then injecting an activation liquid to activate the sperm so that the sperm fertilizes the eggs. During the entire fertilization process, the yellow eel sperm is in direct contact with the eggs before activation, which greatly shortens the migration distance of the yellow eel sperm after activation, allowing the sperm to combine with the eggs in a short time to complete fertilization. Moreover, the yellow eel eggs will not be touched during the entire process, and no damage will be caused to the eggs, thereby greatly improving the fertilization success rate of the eggs. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the internal structure of the artificial insemination device of the present invention Figure 1 ;

[0026] Figure 2 Schematic diagram of the internal structure of the artificial insemination device of the present invention Figure 2 ;

[0027] Figure 3 This is a schematic diagram of the external structure of the artificial insemination device of the present invention;

[0028] Figure 4 This is a schematic structural diagram of the egg tray transmission assembly of the present invention;

[0029] Figure 5 This is a schematic diagram of the structure of the nozzle drive assembly of the present invention;

[0030] Figure 6 Schematic diagram of the activation unit structure of the present invention.

[0031] In the picture:

[0032] 1. Base;

[0033] 2. Nozzle drive assembly; 201. Stand; 202. Second screw drive mechanism; 203. Carrier plate; 204. Atomizing device; 205. Container; 206. Pipeline;

[0034] 3. Egg tray transmission assembly; 301. Machine base; 302. Splash-proof enclosure; 303. No. 1 lead screw transmission mechanism; 304. Tray rack; 305. Egg holding tray;

[0035] 4. Activation unit; 401. Activation liquid storage tank; 402. Delivery pump; 403. Spray pipe;

[0036] 5. Protective shell cover;

[0037] 6. Egg tray removal and placement. DETAILED DESCRIPTION

[0038] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be briefly introduced below in conjunction with the drawings and the description of the embodiments or the prior art. Obviously, the following description of the structure of the drawings is only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0039] Example:

[0040] In response to the problems of the dry and wet fertilization methods currently used for artificial insemination of eel seedlings in artificial aquaculture, which are long time-consuming, have a high incidence of bad eggs, and have a low fertilization rate, we propose a method for artificial insemination of eel eggs and corresponding equipment that is completely different from the current traditional dry and wet fertilization procedures and methods. The specific plan is as follows:

[0041] This embodiment provides a method for artificial fertilization of eel eggs, comprising:

[0042] Step 1: Prepare male and female broodstock, extract eel semen and female eel eggs, and place the extracted eel semen and eggs in sterile containers for later use.

[0043] It needs to be further explained that in order to ensure the activity and quality of the extracted eel sperm, the weight of the male broodstock should be no less than 100 grams each, and when taking eggs from female broodstock, female broodstock with mature eggs should be selected.

[0044] Step 2: Prepare the above-mentioned artificial fertilization device for eel eggs and fish egg hatching equipment. In order to provide a relatively sterile and safe environment for fertilization of eel eggs, it is necessary to implement all-round sterile disinfection inside and outside the equipment. The disinfection of the equipment can be selected but is not limited to ozone disinfection.

[0045] like Figure 1-2 As shown, the artificial insemination device for yellow eel eggs includes a base 1, a nozzle drive assembly 2, an egg tray transmission assembly 3 and an activation unit 4.

[0046] like Figure 5 As shown, the nozzle drive assembly 2 is installed on the top of the base 1, and the nozzle drive assembly 2 includes a stand 201 fixedly installed with the base 1, and a No. 2 screw transmission mechanism 202 is horizontally installed on the stand 201, and a carrier plate 203 is installed at the bottom of the screw slider of the No. 2 screw transmission mechanism 202, and an atomizing device 204 is installed at the end of the carrier plate 203. The liquid inlet port of the atomizing device 204 is connected to the containing tank 205 through a pipe 206, wherein the main function of the nozzle drive assembly 2 is to drive the atomizing device 204 to move back and forth in a horizontal direction above the egg tray transmission assembly 3, and cooperate with the start-up of the atomizing device 204 to ensure that sperm is evenly attached to the egg membrane on the surface of the eel eggs.

[0047] like Figure 1-4 As shown, in order to cooperate with the nozzle drive device and the atomizing device 204 installed thereon to realize the atomized attachment of the eel sperm, and also to facilitate the subsequent taking and placing of the egg holding tray 305 containing the eel eggs, so as to facilitate the subsequent fertilization operation of the eel eggs, the egg tray transmission assembly 3 is arranged at the bottom of the nozzle drive assembly 2, and the egg tray transmission assembly 3 also includes a base 301, a No. 1 screw transmission mechanism 303, a tray rack 304 and an egg holding tray 305 for containing the eel eggs. 05, the egg holding tray 305 is placed on the tray rack 304, the sizes and structures of the tray rack 304 and the egg holding tray 305 are adapted to each other, and the tray rack 304 is horizontally installed on the screw slider assembly of the No. 1 screw transmission mechanism 303; the activation unit 4 includes an activation liquid storage tank 401, a delivery pump 402 and a spray pipe 403; the liquid inlet end of the delivery pump 402 is connected to the activation liquid storage tank 401 through a pipeline, and the liquid outlet of the delivery pump 402 is connected to the spray pipe 403.

[0048] Step 3: Add the eel semen and semen activation liquid into the corresponding holding tank 205 and activation liquid storage tank 401 in the artificial insemination equipment prepared in step 2, in preparation for the eel egg fertilization operation. Before adding the eel semen into the holding tank 205, sperm preservation liquid needs to be added to dilute the eel sperm. The dilution concentration of the eel sperm is 900,000 to 1.1 million sperm / ml.

[0049] Step 4, the fish eggs obtained in step one are contained in the egg holding dish 305, and the egg holding dish 305 is placed on the tray rack 304 in the equipment, the equipment is started, No. 1 screw transmission mechanism 303 in the egg tray transmission assembly 3 is operated, and the tray rack 304 is moved to the position of the spray nozzle drive assembly 2 together with the egg holding dish 305 thereon; the atomizing device 204 in the equipment starts to extract and atomize the yellow eel semen inside the holding tank 205 to make it adhere to the yellow eel egg surface placed in the egg holding dish 305.

[0050] Step 5: After all the eel eggs placed in the egg holding tray 305 have completed the atomization attachment of the eel semen, the activation unit 4 in the equipment is started, and the activation liquid in the activation liquid storage tank 401 is transported to the spray pipe 403, and sprayed into the egg holding tray 305 to activate the eel sperm attached to the surface of the eel eggs and fertilize the eel eggs. When the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius and the fertilization time is 1-2 minutes.

[0051] Step 6: After the fertilization of the yellow eel eggs is completed, they can be placed in the fish egg incubation equipment prepared in step 2 to start hatching. After completing the fertilization operation of all the yellow eel eggs, the equipment is fully cleaned and disinfected again and sealed up for the next fertilization operation.

[0052] The atomizing device 204 in step 2 is composed of a pump and an atomizing nozzle, wherein the atomizing nozzle is installed at the outlet end of the pump, the pipe 206 adopts a disposable sterile tube, and the two ends of the pipe 206 are connected to the container 205 and the atomizing device 204 by quick-release connectors. As is well known, the inner wall of the pipe 206 is actually a narrow space, and its cleaning and disinfection operation is difficult, it is difficult to disinfect thoroughly, and it is easy to breed bacteria. Therefore, this design can reduce the possibility of eel semen being contaminated by the external environment during transportation.

[0053] like Figure 4 As shown, in step 2, in order to prevent the diluted semen from overflowing into the outside of the egg holding tray 305 due to atomization, a splash-proof panel 302 is installed on the periphery of the base 301 of the egg tray transmission assembly 3. Moreover, in order to prevent the eel eggs from stacking and aggregating at the bottom of the tray, the bottom of the egg holding tray 305 is designed to be a square flat-bottom structure, and the two ends of the egg holding tray 305 are slidably attached to the inner side of the splash-proof panel 302.

[0054] like Figure 1 、 Figure 2 、 Figure 6 As shown, in step 2, in order to activate the sperm in time after the sperm atomization and attachment of the eel eggs are completed, and to implement the final fertilization operation of the eel eggs, the activation liquid storage tank 401 and the delivery pump 402 are both installed on one side of the top of the base 1, and the spray pipe 403 is horizontally installed at the front end of the stand 201 in the nozzle drive assembly 2.

[0055] like Figure 3 As shown, in step 2, in order to avoid the influence of the external environment or contamination of the eel sperm and eel eggs as much as possible, a protective shell cover 5 is installed on the top of the base 1, which wraps the nozzle drive assembly 2, the egg tray transmission assembly 3 and the activation unit 4. An egg tray access port 6 is opened at one end of the protective shell cover 5. The egg tray access port 6 is mainly for the convenience of the operator to take and place the egg holding tray 305.

[0056] The difference between the eel egg fertilization method proposed in the present invention and the traditional dry fertilization method is that in the method described in the present invention, atomized and diluted eel semen (90-110 / ml) is used to adhere to the surface of the egg membrane of the fish eggs contained in the egg holding tray 305, which can achieve contact between the sperm and the fish eggs without causing contact between the sperm and the fish eggs, and will not damage the egg membrane on the surface of the fish eggs, thereby ensuring the integrity of the fish eggs to the greatest extent.

[0057] The difference between the present invention and the traditional wet fertilization method of eel eggs is that the eel egg fertilization method proposed in the present invention does not require directly pouring the eel semen and fish eggs into water or activation liquid at the same time. Instead, through the means of atomization attachment, the dormant sperm can first be evenly distributed on the egg membrane of the fish eggs, and then combined with the subsequent addition of activation liquid, the sperm is activated and the fish eggs are fertilized. In this way, the migration distance of the eel sperm after activation can be greatly shortened, and the sperm can be combined with the eggs in a short time to complete fertilization. Moreover, under the premise of the same fertilization rate, the fertilization method provided by the present invention has a sperm demand of about half that of the traditional wet fertilization method.

[0058] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A method for artificial fertilization of eel eggs, comprising: Step 1: Prepare male and female broodstock, extract eel semen and female eel eggs, and place the extracted eel semen and eggs in sterile containers for later use; Step 2: Prepare the artificial fertilization device for yellow eel eggs and the fish egg hatching equipment, and perform all-round sterilization inside and outside the equipment for standby use; The artificial fertilization device for yellow eel eggs comprises a base (1), a nozzle drive assembly (2), an egg tray transmission assembly (3) and an activation unit (4); The nozzle drive assembly (2) is mounted on the top of the base (1), and the nozzle drive assembly (2) includes a stand (201) fixedly mounted on the base (1), a second screw transmission mechanism (202) is horizontally mounted on the stand (201), a carrier plate (203) is mounted on the bottom of the screw slider of the second screw transmission mechanism (202), an atomizing device (204) is mounted on the end of the carrier plate (203), and a liquid inlet port of the atomizing device (204) is connected to the container (205) through a pipe (206); The egg tray transmission assembly (3) is located at the bottom of the nozzle drive assembly (2), and the egg tray transmission assembly (3) includes a machine base (301), a No. 1 screw transmission mechanism (303), a tray rack (304) and an egg holding tray (305); the egg holding tray (305) is placed on the tray rack (304), and the tray rack (304) is horizontally mounted on the screw slider assembly of the No. 1 screw transmission mechanism (303); The activation unit (4) comprises an activation liquid storage tank (401), a delivery pump (402) and a spray pipe (403); the liquid inlet of the delivery pump (402) is connected to the activation liquid storage tank (401) via a pipeline, and the liquid outlet of the delivery pump (402) is in communication with the spray pipe (403); Step 3: Add the eel semen and semen activation liquid into the corresponding holding tank (205) and activation liquid storage tank (401) of the artificial insemination equipment prepared in step 2, respectively, to prepare for the eel egg fertilization operation; Step 4: The fish eggs obtained in step 1 are placed in an egg holding tray (305), and the egg holding tray (305) is placed on a tray rack (304) in the device, the device is started, and the No. 1 screw transmission mechanism (303) in the egg tray transmission assembly (3) is operated to move the tray rack (304) and the egg holding tray (305) thereon to the position of the nozzle driving assembly (2); the atomizing device (204) in the device is started to extract and atomize the yellow eel semen inside the holding tank (205) so that it adheres to the surface of the yellow eel eggs held in the egg holding tray (305); Step 5: After all the eel eggs placed in the egg holding tray (305) have completed the atomization attachment of the eel sperm, the activation unit (4) in the device is started, and the activation liquid in the activation liquid storage tank (401) is transported to the spray pipe (403), and sprayed into the egg holding tray (305), thereby activating the eel sperm attached to the surface of the eel eggs and fertilizing the eel eggs; Step 6: After the fertilization of the yellow eel eggs is completed, they can be placed in the fish egg incubation equipment prepared in step 2 to start hatching. After completing the fertilization operation of all the yellow eel eggs, the equipment is fully cleaned and disinfected again and sealed up for the next fertilization operation.

2. The method for artificial insemination of eel eggs according to claim 1, wherein: The atomizing device (204) in step 2 is composed of a pump and an atomizing nozzle, the pipeline (206) is a disposable sterile tube, and the two ends of the pipeline (206) are connected to the container (205) and the atomizing device (204) by quick-release connectors.

3. The method for artificial insemination of eel eggs according to claim 1, wherein: The base (301) of the egg tray transmission assembly (3) in the step 2 is provided with a splash-proof enclosure (302) on the periphery thereof, and the egg holding tray (305) is designed as a square flat bottom tray, with both ends of the egg holding tray (305) slidingly attached to the inner side of the splash-proof enclosure (302).

4. The method for artificial insemination of eel eggs according to claim 1, wherein: The activation liquid storage tank (401) and the delivery pump (402) in step 2 are both installed on one side of the top of the base (1), and the spray pipe (403) is installed horizontally at the front end of the stand (201) in the nozzle drive assembly (2).

5. The method for artificial insemination of eel eggs according to claim 1, wherein: A protective shell (5) is installed on the top of the base (1) in step 2, which encloses the nozzle drive assembly (2), the egg tray transmission assembly (3) and the activation unit (4). One end of the protective shell (5) is provided with an egg tray access opening (6).

6. The method for artificial insemination of eel eggs according to claim 1, wherein: In the step three, before the eel semen is added into the container (205), a sperm preservation solution is added to dilute the eel sperm, and the dilution concentration of the eel sperm is 900,000 to 1.1 million sperm / ml.

7. The method for artificial insemination of eel eggs according to claim 1, wherein: In the step 5, when the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius and the fertilization time is 1-2 minutes.

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