Insecticide composition and its application

Abstract

The present invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition and its use. The insecticidal composition comprises active ingredients A and B, wherein the active ingredient A is a compound represented by Formula I; the active ingredient B is any one of tetrazolyl fenpropiamide, cyclobromofenapyr, cyantraniliprole, flufenapyr, chlorfenapyr, or flubendiamide; and the mass ratio of active ingredient A to active ingredient B is 1:45 to 45:1. The insecticidal composition of the present invention rationally combines compounds with different mechanisms of action to produce a synergistic effect, thereby improving pest control effectiveness, reducing the application rate, reducing the number of applications, and lowering the cost of use.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide compounding, and particularly relates to an insecticide composition and its application in preventing and controlling pests in agricultural or non-agricultural environments. Background Art

[0002] Diamide insecticides have been a hot topic in the field of insecticide research in recent years. They mainly act on the ryanodine receptors of insects. Their mechanism of action is novel, highly effective, and has no cross-resistance with traditional pesticides.

[0003] In recent years, the incidence of various crop pests has been increasing year by year, with the affected areas expanding year by year. This has caused significant damage to crops, leading to reduced crop yields and increased agricultural production costs. To improve pest control, farmers have increased the dosage of diamide insecticides or arbitrarily mixed them. This unscientific use of pesticides not only fails to achieve synergistic effects, but also leads to pesticide waste, excessive residues, environmental pollution, and the development of pesticide resistance in pests.

[0004] In view of the current situation that pests have developed resistance to diamide insecticides, resulting in an increase in pesticide usage year by year, while the control effect is showing a downward trend, screening out new high-efficiency, low-toxic, green and environmentally friendly control agents can provide a theoretical basis for the integrated control of pests. Therefore, this experiment selected the compound of formula I and 6 kinds of diamide insecticides for reasonable compounding, measured their biological activity against various pests indoors, and carried out corresponding field efficacy tests, aiming to screen for agents and dosages with better control effects on target pests, which can provide scientific drug use guidance for the control of various agricultural and non-agricultural pests. At the same time, it is of great significance to the long-term and sustainable management of pests and the reduction of crop damage. Summary of the Invention

[0005] In order to solve the above problems existing in the prior art, the present invention provides an insecticide composition and its application. The insecticide composition has excellent control effects on various agricultural and non-agricultural pests, reduces the dosage of pesticides, and slows down the development of pest resistance.

[0006] In order to achieve the above objectives, the present invention adopts the following technical solution: an insecticide composition, wherein the active ingredients of the insecticide composition include active ingredient A and active ingredient B, and the active ingredient A is a compound represented by formula I:

[0007] The active ingredient B is any one of tetrazobactam, cyclofenac, cyantraniliprole, flubendiamide, chlorantraniliprole, and flubendiamide; the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 45:1, or any value within the above numerical range.

[0008] Furthermore, the active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 30:1, or any value within the above numerical range;

[0009] The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:1, or any value within the above numerical range;

[0010] The active ingredient B is cyantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1, or any value within the above numerical range;

[0011] The active ingredient B is flufenacet, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:1, or any value within the above numerical range;

[0012] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 45:1, or any value within the above numerical range;

[0013] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 32:1, or any value within the above numerical range;

[0014] Furthermore, the active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1, or any value within the above numerical range;

[0015] The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 20:1, or any value within the above numerical range;

[0016] The active ingredient B is cyantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 18:1, or any value within the above numerical range;

[0017] The active ingredient B is flufenacet, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 24:1, or any value within the above numerical range;

[0018] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 32:1, or any value within the above numerical range;

[0019] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 24:1, or any value within the above numerical range.

[0020] Furthermore, the active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 25:1, or any value within the above numerical range;

[0021] The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 10:1, or any value within the above numerical range;

[0022] The active ingredient B is cyantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1, or any value within the above numerical range;

[0023] The active ingredient B is flufenacet, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 16:1, or any value within the above numerical range;

[0024] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 24:1, or any value within the above numerical range;

[0025] The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 14:1, or any value within the above numerical range.

[0026] Furthermore, in addition to the active ingredients, the insecticide composition also includes other pesticide-acceptable adjuvants, and the adjuvants are selected from one or more of dispersants, wetting agents, emulsifiers, stabilizers, antifreeze agents, defoaming agents, preservatives, thickeners, solvents, organic acids, and dispersion media.

[0027] Furthermore, the composition can be prepared into various dosage forms, including wettable powders, water-dispersible granules, suspensions, aqueous emulsions, suspension seed coatings, microcapsule suspensions, microcapsule suspension-suspension concentrates, emulsifiable concentrates, microemulsions, dispersible liquids or granules.

[0028] The present invention also discloses the use of the insecticide composition for preventing and controlling agricultural or non-agricultural pests.

[0029] Furthermore, the pests are Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites.

[0030] Furthermore, the pests are Lepidoptera, Thysanoptera, and Hemiptera pests;

[0031] In particular, the pesticide composition of the present invention has excellent control effects on diamondback moth, cotton bollworm, corn borer, corn armyworm, fall armyworm, beet armyworm, rice leaf roller, Spodoptera exigua, thrips and aphids.

[0032] The present invention also discloses a method for controlling invertebrate pests, comprising contacting the invertebrate pests or their environment with a biologically effective amount of the composition described above.

[0033] The present invention also discloses a method for preventing and controlling insect pests in agriculture or non-agriculture, comprising applying the above composition to plants where the pests exist.

[0034] The beneficial effects of the present invention are as follows:

[0035] The present invention rationally compounds insecticide compounds with different action mechanisms, has a synergistic effect on multiple pests, can significantly improve the pest control effect, and expand the insecticide spectrum; while delaying the development of pest resistance, it also reduces the amount of pesticide applied, reduces the number of pesticide applications, and reduces production costs. DETAILED DESCRIPTION

[0036] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0037] Preparation example:

[0038] Preparation Example 1: 24% Formula I compound·tetrazolyl tetrazolamide suspension (5:1)

[0039] Formula: 20% compound of formula I, 4% tetrazolyl amide, 2% isomeric tridecanol polyoxyethylene ether, 4% styrenated phenol polyoxyethylene ether phosphate, 2% sodium polycarboxylate, 0.25% xanthan gum, 5% ethylene glycol, 1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0040] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0041] Preparation Example 2: 28% Formula I compound·tetrazolylcarboxamide water dispersible granules (25:3)

[0042] Formula: 25% compound of formula I, 3% tetrazolyl amide, 5% sodium lignin sulfonate, 2.5% flavonoid powder BX, 12% sodium polycarboxylate, 5% white sugar, and kaolin to make up the balance.

[0043] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and screening are carried out to obtain water-dispersible granule products; or the ground powder is sprayed with water in a boiling granulator, granulated, dried, and then screened to obtain water-dispersible granule products.

[0044] Preparation Example 3: 33% Formula I compound·tetrazolyl thiabendazole wettable powder (1:10)

[0045] Formula: 3% compound of formula I, 30% tetrazolyl amide, 2% sodium lauryl sulfate, 10% sodium lignin sulfonate, 2% naphthalene sulfonate formaldehyde condensate, 5% dispersant NNO, 5% white carbon black, and kaolin to make up the balance;

[0046] Preparation method: According to the formula composition, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0047] Preparation Example 4: 18% Formula I compound·Cyclofenac suspension (5:1)

[0048] Formula: 15% compound of formula I, 3% cyclobromofenapyr, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% fatty alcohol polyoxyethylene ether phosphate, 2% polyoxyethylene sorbitan monooleate, 1% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 0.5% isothiazolinone, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0049] Preparation method: Same as Preparation Example 1.

[0050] Preparation Example 5: 26% Formula I compound·Cyclofenac water dispersible granules (1:12)

[0051] Formula: 2% compound of formula I, 24% cyclobromofenapyr, 10% lignin sulfonate, 8% naphthalene sulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 5% white carbon black, 30% starch, and kaolin makes up the balance.

[0052] Preparation method: Same as Preparation Example 2.

[0053] Preparation Example 6: 25% Formula I compound·Cyclofenac wettable powder (3:2)

[0054] Formula: 15% compound of formula I, 10% cyclobromofenapyr, 3% sodium lauryl sulfate, 5% sodium lignin sulfonate, 6% sodium polynaphthaldehyde sulfonate, 5% kaolin, 8% white carbon black, and bentonite makes up the balance;

[0055] Preparation method: Same as Preparation Example 3.

[0056] Preparation Example 7: 16% Formula I compound·cyantraniliprole suspension concentrate (1:3)

[0057] Formula: 4% compound of formula I, 12% cyantraniliprole, 1% sodium lignin sulfonate, 1% naphthalene sulfonate formaldehyde condensate, 3% castor oil polyoxyethylene ether, 2% sodium alkyl polyoxyethylene ether sulfonate, 4% fatty alcohol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0058] Preparation method: Same as Preparation Example 1.

[0059] Preparation Example 8: 27% Formula I compound·cyantraniliprole water dispersible granules (8:1)

[0060] Formula: 24% compound of formula I, 3% cyantraniliprole, 8% naphthalenesulfonate formaldehyde condensate, 10% sodium ligninsulfonate, 3% sodium lauryl sulfate, 8% ammonium sulfate, and starch makes up the balance;

[0061] Preparation method: Same as Preparation Example 2.

[0062] Preparation Example 9: 20% Formula I compound·cyantraniliprole dispersible oil suspension (1:9)

[0063] Formula: 2% compound of formula I, 18% cyantraniliprole, 2% lignin sulfonate, 10% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 2% castor oil polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 0.5% silicon dioxide, 0.5% organobentonite, 10% 200# solvent oil, and methyl oleate to make up the balance;

[0064] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0065] Preparation Example 10: 20% Formula I compound·flufenacil suspension (4:1)

[0066] Formula: 16% compound of formula I, 4% flufenacet, 3% ethylene glycol oxyethylene polyoxypropylene ether, 2% phenylethylphenol polyoxyethylene polyoxypropylene ether, 2% tristyrylphenol ethoxylate phosphate, 2% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% potassium benzoate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0067] Preparation method: Same as Preparation Example 1.

[0068] Preparation Example 11: 3.25% Formula I compound·flufenamid aqueous emulsion (12:1)

[0069] Formula: 3% compound of formula I, 0.25% flufenacet, 5% phenylethylphenol polyoxyethylene polyoxypropylene ether, 3% styrenated phenol polyoxyethylene ether phosphate, 18% cyclohexanone, 12% acetophenone, 0.2% xanthan gum, 0.1% potassium benzisothiazolinone, 5% ethylene glycol, 2% urea, 0.5% sodium sorbate, 0.1% organosilicon defoamer, and deionized water to make up the balance;

[0070] Preparation method: According to the formula ratio, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water, antifreeze, etc. are mixed together to form a uniform water phase. Under high-speed shearing, the oil phase is added to the water phase. After shearing to a qualified particle size, a defoamer, a thickener, and a preservative are added and stirred evenly to form a well-dispersed water emulsion product.

[0071] Preparation Example 12: 3% Formula I compound·flufenacil emulsifiable concentrate (1:9)

[0072] Formula: 0.3% compound of formula I, 2.7% flufenacet, 10% acetophenone, 20% EO / PO block copolymer, 8% N-octylpyrrolidone, 1% calcium dodecylbenzenesulfonate, 12% cyclohexanone, and xylene makes up the balance.

[0073] Preparation method: According to the formula ratio of the preparation example, the measured active ingredients, solvent, and cosolvent are added to a mixing kettle and stirred to dissolve them, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle. After filtering, the emulsifiable concentrate of the present invention is obtained.

[0074] Preparation Example 13: 18% Formula I compound·Chlorantraniliprole suspension concentrate (9:1)

[0075] Formula: 16.2% compound of formula I, 1.8% chlorantraniliprole, 1% glycerol fatty acid ester polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether sulfate, 4% tristyrylphenol ethoxylate phosphate, 2% triphenylphenol polyoxyethylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0076] Preparation method: Same as Preparation Example 1.

[0077] Preparation Example 14: 22% Formula I compound·Chlorantraniliprole water dispersible granules (1:10)

[0078] Formula: 2% compound of formula I, 20% chlorantraniliprole, 3% sodium lauryl sulfate, 8% naphthalenesulfonate formaldehyde condensate, 5% sodium salt of polycarboxylate, 5% white sugar, and kaolin makes up the balance.

[0079] Preparation method: Same as Preparation Example 2.

[0080] Preparation Example 15: 13.5% Formula I compound·Chlorantraniliprole dispersible oil suspension (22:5)

[0081] Formula: 11% compound of formula I, 2.5% chlorantraniliprole, 4% fatty alcohol polyoxyethylene ether, 2% sorbitan polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 9% castor oil polyoxyethylene ether, 1% sodium polycarboxylate, 2% sodium alkyl polyoxyethylene ether sulfonate, 1% organobentonite, 1% silicon dioxide, and soybean oil makes up the balance;

[0082] Preparation method: Same as Preparation Example 9.

[0083] Preparation Example 16: 20% Formula I compound·flubenzuronide suspension (9:1)

[0084] Formula: 18% compound of formula I, 2% flubendiamide, 2% alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% castor oil polyoxyethylene ether, 3% tristyrylphenol ethoxylate phosphate, 1% sodium salt of polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium benzoate, 5% glycerol, 0.5% silicone oil, and deionized water to make up the balance;

[0085] Preparation method: Same as Preparation Example 1.

[0086] Preparation Example 17: 32% Formula I compound·flubenzuronil water dispersible granules (3:1)

[0087] Formula: 24% compound of formula I, 8% flubendiamide, 8% sodium lignin sulfonate, 2% flavonoid powder BX, 10% naphthalenesulfonate formaldehyde condensate, 5% white sugar, and kaolin to make up the balance;

[0088] Preparation method: Same as Preparation Example 2.

[0089] Preparation Example 18: 30% Formula I compound·flubenzuronil wettable powder (1:5)

[0090] Formula: 5% compound of formula I, 25% flubendiamide, 5% sodium lignin sulfonate, 5% dispersant NNO, 2.5% pulverized powder BX, 5% white carbon black, and kaolin to make up the balance;

[0091] Preparation method: Same as Preparation Example 3.

[0092] Indoor toxicity assay example

[0093] Test targets: Diamondback moth, Fall armyworm, Spodoptera litura, Corn armyworm, Cotton bollworm, Thrips, Aphids;

[0094] Test agents: tetrazobactam, cyclofenac, cyantraniliprole, flubendiamide, chlorantraniliprole, flubendiamide, and the technical drug of the compound of formula I.

[0095] 1. Lepidoptera pest assay method: Toxicity assays were conducted on third-instar larvae of Plutella xylostella, Spodoptera frugiperda, Corn Armyworm, and Helicoverpa armigera, in accordance with the Pesticides Part 14: Leaf Dip Method, as per the Agricultural Industry Standard of the People's Republic of China, NY / T 1154.14-2008, "Guidelines for Indoor Pesticide Bioassays."

[0096] First, prepare the test agent in a suitable solvent to a gradient of five concentrations. A series of mass ratios of the mixed agents are then set, and the final concentrations are determined using these ratios. Appropriate leaf discs are immersed in the test agent solution for 10 seconds, removed and air-dried, and placed in a Petri dish containing moisturizing filter paper. Test insects are then seeded, with 20 insects per replicate. Each treatment is replicated four times, with a blank control containing no agent (including all organic solvents and emulsifiers).

[0097] 48 hours after treatment, examine the insects for mortality and record the total number of insects and the number of dead insects. Gently touch the insect body with tweezers, and the lack of reaction is considered the criterion for death.

[0098] 2. Determination method for Hemiptera and Thysanoptera pests: Refer to the agricultural industry standard of the People's Republic of China NY / T1154.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides" Part 6: Insect Dipping Method, and conduct toxicity tests on cotton aphids (wingless adults) and rice thrips (adults).

[0099] First, prepare the test agent in a suitable solvent to form five concentration gradients. The mixed agents are then mixed in a series of ratios, and the final mass concentrations are prepared according to the different ratios. Target insects are immersed in the corresponding agent solution for 5 seconds. Excess solution is then removed with filter paper, and the test insects are transferred to normal conditions for rearing. Each treatment is replicated four times, with 20 insects immersed in each replicate. A treatment containing no agent (including all organic solvents and emulsifiers) serves as a blank control.

[0100] 48 h after treatment, the mortality of the test insects was investigated and the number of dead insects and the total number of insects were recorded.

[0101] The above experiment calculates the mortality rate of each treatment based on the survey data. Calculate according to the following formula:

[0102]

[0103] Where:

[0104] P——mortality rate, in percentage (%);

[0105] K——indicates the number of dead insects, the unit is head;

[0106] N——represents the total number of insects processed, in heads.

[0107]

[0108] Where:

[0109] P1——adjusted mortality rate, in percentage (%);

[0110] P t ——Treatment mortality rate, expressed in percentage (%);

[0111] P0 - blank control mortality rate, in percentage (%).

[0112] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0113] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0114] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0115]

[0116] Where:

[0117] ATI - measured toxicity index of mixture;

[0118] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0119] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0120] TTI=TI A ×P A +TI B ×P B

[0121] Where:

[0122] TTI – Theoretical Toxicity Index of Mixtures;

[0123] TI A ——Agent toxicity index;

[0124] P A——The percentage of agent A in the mixture, in percentage (%);

[0125] TI B ——Toxicity index of agent B;

[0126] P B ——The percentage of agent B in the mixture, in percentage (%).

[0127]

[0128] Where:

[0129] CTC – Co-toxicity coefficient;

[0130] ATI - measured toxicity index of mixture;

[0131] TTI - Theoretical Toxicity Index of Mixture.

[0132] According to the Sun Yunpei method, the co-toxicity coefficients of different ratios of two drugs were calculated. A co-toxicity coefficient of CTC ≥ 120 indicated a synergistic effect; CTC ≤ 80 indicated an antagonistic effect; and 80 < CTC < 120 indicated an additive effect.

[0133] Indoor pesticide activity test for Plutella xylostella:

[0134] Example 1: To verify the control effect of different ratios of the compound of formula I and flubendiamide on the diamondback moth, indoor toxicity tests on the diamondback moth were conducted using different ratios of the compound of formula I and flubendiamide. The corresponding concentrations were prepared according to the mass ratios in the table and indoor insecticidal tests were conducted. The test results are as follows:

[0135] Table 1 Indoor biological activity test results of different ratios of compound I and flubendiamide against Plutella xylostella

[0136]

[0137]

[0138] The results of the indoor tests (see Table 1) show that the mass ratio of the compound of formula I to flufenacet is 1:32 to 30:1, and the co-toxicity coefficient to the diamondback moth is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to flufenacet is 1:24 to 24:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; the mass ratio of the compound of formula I to flufenacet is 1:18 to 24:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0139] Example 2: To verify the control effect of different ratios of the compound of formula I and flubendiamide on the diamondback moth, indoor toxicity tests on the diamondback moth were conducted using different ratios of the compound of formula I and flubendiamide. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0140] Table 2 Indoor biological activity test results of different ratios of compound I and flubendiamide against Plutella xylostella

[0141]

[0142] The results of the indoor tests (see Table 2) show that the mass ratio of the compound of formula I to flubendiamide is 1:25-32:1, and the co-toxicity coefficient to the diamondback moth is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to flubendiamide is 1:15-24:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; the mass ratio of the compound of formula I to flubendiamide is 1:10-24:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0143] Indoor test on the activity of pesticides against corn armyworm

[0144] Example 3: To verify the control effect of different ratios of the compound of formula I and cyclobromofenamide on corn armyworms, indoor toxicity tests on corn armyworms were conducted using different ratios of the compound of formula I and cyclobromofenamide. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0145] Table 3 Indoor biological activity test results of different ratios of compound I and cyclobromofenapyr against corn armyworm

[0146]

[0147] The results of the indoor tests (see Table 3) show that the mass ratio of the compound of formula I and cyclic bromine sulfamethoxazole to corn armyworm is 1:32-30:1, and the co-toxicity coefficient is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I and cyclic bromine sulfamethoxazole is 1:24-20:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; the mass ratio of the compound of formula I and cyclic bromine sulfamethoxazole is 1:12-10:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0148] Example 4: To verify the control effect of different ratios of the compound of formula I and cyantraniliprole on corn armyworms, indoor toxicity tests on corn armyworms were conducted using different ratios of the compound of formula I and cyantraniliprole. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0149] Table 4 Indoor biological activity test results of different ratios of compound I and cyantraniliprole against corn armyworm

[0150]

[0151] The results of the indoor tests (see Table 4) show that the mass ratio of the compound of formula I to cyantraniliprole is 1:28-30:1, and the co-toxicity coefficient against corn armyworm is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to cyantraniliprole is 1:22-24:1, and the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to cyantraniliprole is 1:18-16:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is significant.

[0152] Indoor test on the activity of pesticides against cotton bollworm

[0153] Example 5: To verify the control effect of different ratios of the compound of formula I and tetrazolin on cotton bollworm, indoor toxicity tests on cotton bollworm were conducted using different ratios of the compound of formula I and tetrazolin. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0154] Table 5 Indoor biological activity test results of different ratios of compound I and tetrazolyl thiamethoxam against cotton bollworm

[0155]

[0156] The results of the indoor tests (see Table 5) show that the mass ratio of the compound of formula I to tetrazolin is 1:20-30:1, and the co-toxicity coefficient against cotton bollworm is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to tetrazolin is 1:10-20:1, and the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to tetrazolin is 1:5-20:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is significant.

[0157] Example 6: To verify the control effect of different ratios of the compound of formula I and chlorantraniliprole on cotton bollworm, indoor toxicity tests on cotton bollworm were conducted using different ratios of the compound of formula I and chlorantraniliprole. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0158] Table 6 Indoor biological activity test results of different ratios of compound I and chlorantraniliprole against cotton bollworm

[0159]

[0160]

[0161] The results of the indoor tests (see Table 6) show that the mass ratio of the compound of formula I to chlorantraniliprole is 1:36-32:1, and the co-toxicity coefficient to cotton bollworm is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to chlorantraniliprole is 1:25-24:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; the mass ratio of the compound of formula I to chlorantraniliprole is 1:14-18:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0162] Indoor test on the activity of pesticides against fall armyworm

[0163] Example 7: To verify the control effect of different ratios of the compound of formula I and tetrazolin on fall armyworm, indoor toxicity tests on fall armyworm were conducted using different ratios of the compound of formula I and tetrazolin. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0164] Table 7 Indoor biological activity test results of different ratios of the compound of formula I and tetrazolyl amide against fall armyworm

[0165]

[0166] The results of the indoor tests (see Table 7) show that the mass ratio of the compound of formula I to tetrazolin is 1:24 to 30:1, and the co-toxicity coefficient against the fall armyworm is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to tetrazolin is 1:24 to 25:1, and the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to tetrazolin is 1:12 to 20:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is significant.

[0167] Example 8: To verify the control effect of different ratios of the compound of formula I and chlorantraniliprole on fall armyworm, indoor toxicity tests on fall armyworm were conducted using different ratios of the compound of formula I and chlorantraniliprole. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0168] Table 8 Indoor biological activity test results of different ratios of the compound of formula I and chlorantraniliprole against fall armyworm

[0169]

[0170]

[0171] The results of the indoor tests (see Table 8) show that the mass ratio of the compound of formula I to chlorantraniliprole is 1:30-45:1, and the co-toxicity coefficient to the fall armyworm is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to chlorantraniliprole is 1:22-35:1, and the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to chlorantraniliprole is 1:15-20:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is significant.

[0172] Indoor test on the activity of pesticides against rice thrips

[0173] Example 9: To verify the control effect of different ratios of the compound of formula I and cyantraniliprole on rice thrips, indoor toxicity tests on rice thrips were conducted using different ratios of the compound of formula I and cyantraniliprole. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0174] Table 9 Indoor biological activity test results of different ratios of compound I and cyantraniliprole against rice thrips

[0175]

[0176] The results of the indoor tests (see Table 9) show that the mass ratio of the compound of formula I to cyantraniliprole is 1:30 to 25:1, and the co-toxicity coefficient against rice thrips is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to cyantraniliprole is 1:20 to 20:1, and the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to cyantraniliprole is 1:10 to 10:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0177] Indoor test on the activity of pesticides against cotton aphids

[0178] Example 10: To verify the control effect of different ratios of the compound of formula I and cyantraniliprole on cotton aphids, indoor toxicity tests on cotton aphids were conducted using different ratios of the compound of formula I and cyantraniliprole. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal tests were conducted. The test results are as follows:

[0179] Table 10 Indoor bioactivity test results of different ratios of compound I and cyantraniliprole against cotton aphids

[0180]

[0181] The results of the indoor tests (see Table 10) show that the mass ratio of the compound of formula I to cyantraniliprole is 1:27-18:1, and the co-toxicity coefficient to cotton aphids is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to cyantraniliprole is 1:15-9:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; the mass ratio of the compound of formula I to cyantraniliprole is 1:9-9:1, and the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0182] Field efficacy trials

[0183] Example 11: Field efficacy test for controlling diamondback moth

[0184] The test was conducted in accordance with the "Guidelines for Field Efficacy Tests of Pesticides (I) Part 13: Insecticides for Control of Lepidoptera Larvae on Cruciferous Vegetables"

[0185] (GB / T17980.13-2000).

[0186] The trial took place at a broccoli planting base in Wangtian Village, Helan County, Yinchuan City, Ningxia Hui Autonomous Region. The broccoli variety was "Excellent Cold Resistance." The trial site was flat and well-maintained.

[0187] Experimental method: The experiment set up 14 treatments, each treatment was repeated 4 times, and the area of ​​each plot was 20m 2 At the beginning of the peak period of the diamondback moth larvae (1st to 2nd instar), a backpack electric sprayer (PJB-16) with a conical nozzle, a pressure of 0.41 MPa, a flow rate of about 0.78 L / min, and a spray method were used for one application. The water volume was about 45 L / 667 m 2 To prevent the liquid from drifting, spray the pesticide alternately between each treatment.

[0188] Ten broccoli plants were randomly fixed and marked in each plot, and the number of live larvae of different instars on the entire plant was investigated. The baseline insect population was surveyed before application, and the number of remaining insects on the fixed plants and crop safety were measured 3 and 10 days after application.

[0189] Calculation method of drug efficacy:

[0190]

[0191]

[0192] Table 11 Field efficacy test results for controlling diamondback moth

[0193]

[0194] According to the results of the field efficacy test, the insect population base was investigated 3 days after the application, and the control effect was calculated. It was found that the effect of Preparation Example 10: 20% Formula I compound·flufenacil suspension (4:1) was 16 g / hm2. 2 The best control effect was achieved, with a control effect of 92.06%. Ten days after application, the insect population base was surveyed. The control effects of the treatment groups treated with Preparation Example 10: 20% Formula I compound·flubenzuron amide suspension concentrate (4:1), Preparation Example 17: 32% Formula I compound·flubenzuron amide water dispersible granules (3:1), Preparation Example 2: 28% Formula I compound·tetrazobactam water dispersible granules (25:3), and Preparation Example 7: 16% Formula I compound·cyantraniliprole suspension concentrate (1:3) all increased compared with 3 days after application. Among them, Preparation Example 10: 20% Formula I compound·flubenzuron amide suspension concentrate (4:1) and Preparation Example 17: 32% Formula I compound·flubenzuron amide water dispersible granules (3:1) had the best control effects, reaching 95.40% and 93.51%, respectively.

[0195] Safety investigation: During the field trial, no pesticide damage occurred in any treatment group, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area, indicating that the test agents were safe for broccoli growth at the doses provided.

[0196] Example 12: Field efficacy test for controlling corn armyworm and fall armyworm

[0197] The test site is Dazhuangsi Village, Liuquanpu Town, Zhenping County, Henan Province. The soil in the test site is yellow loam with medium soil fertility. The growth and growth period of corn plants in the test site are consistent.

[0198] Experimental crop: corn (Qin Xin 1708).

[0199] Target of prevention and control: Fall armyworm and corn borer occur together, with fall armyworm being the main target.

[0200] Experimental design: The experiment set up 14 treatments, each treatment was repeated 4 times, and the area of ​​each plot was 50m 2 , randomized block arrangement. Different treatments should be arranged with protection rows around four weeks.

[0201] Test method: The test was carried out in the corn bell stage, and the medicine was prepared by double dilution method. First, the medicine was diluted with 100mL of clean water, and then shaken thoroughly to form a mother solution. 2 For standard water usage, first pour half clean water into the sprayer, then add the mother liquor, then pour the remaining half clean water into the sprayer. Stir the liquid thoroughly and spray evenly. Use a backpack electric sprayer to spray the corn leaves, especially the heart leaves. To ensure uniform application and reduce errors caused by the application tools or the operator's skill level, all spraying operations should be completed by a single experienced person.

[0202] Survey time and method: A five-point sampling method was used, with 5 plants surveyed at each point and a total of 25 plants surveyed for each treatment. A total of 3 surveys were conducted during the entire trial period. The insect population base was surveyed before the application of the drug, and the number of live insects on the plants was surveyed and recorded 7 and 14 days after the application of the drug.

[0203] Calculation method of drug efficacy:

[0204]

[0205] Table 12 Results of field trials on efficacy of pesticides against corn armyworm and fall armyworm

[0206]

[0207] The results showed that the combination formulations significantly outperformed the single-dose control formulations in both rapid-acting and sustained effectiveness against corn armyworm and fall armyworm. Seven days after application, the best control was achieved with the formulation (Example 8): 27% Formula I compound·cyantraniliprole water dispersible granules (8:1) at 92.53%. Fourteen days after application, the formulation (Example 6): 25% Formula I compound·cyantraniliprole wettable powder (3:2) at 94.05%.

[0208] Example 13: Field efficacy test for controlling rice thrips

[0209] Experimental location: The experiment was located in the rice fields of Kongxiang Village, Shuitian Township, Jishui County, Ji'an City, Jiangxi Province. The fertility of the experimental land was above average, and the cultivation and management conditions of all experimental plots were consistent.

[0210] Experimental design: The experiment set up 5 drug treatments and 1 blank control, and the area of ​​each experimental plot was 25m 2 Each treatment was repeated 4 times, and all experimental plots were arranged in random blocks, with ridges built between the plots.

[0211] Test method: Apply the pesticide once 10 days after rice transplanting. Prepare the solution from low concentration to high concentration according to the test design. Use 45kg / 667m 2 .

[0212] Survey method: Survey the insect population base before spraying, and survey the number of live insects 3 days, 7 days, and 14 days after spraying. Survey 10 points in each plot using the parallel jump method, with each point surveying 0.1m 2 , investigate the residual insect quantity, calculate the insect population reduction rate and correct the control effect.

[0213] Calculation method of drug efficacy:

[0214]

[0215] Table 13 Field efficacy test results for controlling rice thrips

[0216]

[0217] The results of the field efficacy test show that 3 days after application, the control effects of the three combination treatment groups of Preparation Example 7: 16% Formula I compound·cyantraniliprole suspension concentrate (1:3), Preparation Example 9: 20% Formula I compound·cyantraniliprole dispersible oil suspension concentrate (1:9), and Preparation Example 8: 27% Formula I compound·cyantraniliprole water-dispersible granules (8:1) were 88.82%, 85.50%, and 83.52%, respectively, which were higher than the control effects of the control agents 25% Formula I compound wettable powder and 10% cyantraniliprole suspension concentrate; 7 days after application, the control effects of the three combination treatment doses of the three Formula I compounds and cyantraniliprole reached 90.35%, 89.30% and 86.64%, respectively; 14 days after application, the control effects of the three combination treatment doses of the three Formula I compounds and cyantraniliprole were 91.74%, 89.05% and 84.87%, respectively.

[0218] Example 14: Field efficacy test for controlling wolfberry cotton aphids

[0219] The test site is Xishagou Village, Keke Town, Ulan County, Haixi Prefecture, Qinghai Province. The soil type of the test site is sandy loam, the terrain is flat, the soil fertility of the test site is consistent, and the drainage and irrigation are good.

[0220] Experimental design: The experiment set up 6 treatments, each treatment was repeated 4 times, and the area of ​​each plot was 30m 2 , each plot was arranged in random blocks.

[0221] Test Method: A single application of pesticides was conducted during the wolfberry fruiting stage using a 3WBD-20L electric backpack sprayer. All test pesticides were prepared on-site, diluted using a two-step dilution method, and sprayed evenly until a small amount of water dripped from the leaves.

[0222] Survey Method: Randomly survey two wolfberry trees in each plot. Each tree was surveyed from five locations (east, south, west, north, and center). Five branches were randomly surveyed, and the number of live insects remaining on each branch, 30 cm below the top, was recorded. The baseline insect population was surveyed before treatment, and the number of live insects was surveyed 3 and 7 days after treatment. The population reduction rate and control efficacy were calculated based on the survey results.

[0223] Calculation method of drug efficacy:

[0224]

[0225] The test results are shown below:

[0226] Table 14 Field efficacy test results for controlling wolfberry cotton aphid

[0227]

[0228] The results of the field efficacy test show that the combination of Formula I compound and cyantraniliprole has a good field control effect on wolfberry cotton aphid. Test agent preparation example 8: 27% Formula I compound cyantraniliprole water dispersible granules (8:1), preparation example 9: 20% Formula I compound cyantraniliprole dispersible oil suspension (1:9), preparation example 7: 16% Formula I compound cyantraniliprole suspension (1:3) 3 treatments 35g / hm 2 Three days after application, the target pest populations were significantly reduced, with population reduction rates of 83.89%, 88.14%, and 90.73%, respectively, and control efficacy rates of 84.35%, 88.48%, and 90.99%, respectively. These rates were significantly higher than those of the control agents: a 25% wettable powder of the compound of Formula I and a 10% cyantraniliprole suspension concentrate. Seven days after application, the 16% cyantraniliprole suspension concentrate (1:3) formulated in Example 7 demonstrated the highest control efficacy of 94.5%.

[0229] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition, characterized in that The active ingredients of the insecticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula I: (Formula I), the active ingredient B is any one of tetrazobactam, cyclobromofenapyr, cyantraniliprole, flubendiamide, and flubendiamide; The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20-30:1; The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:1; The active ingredient B is cyantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 18:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25~32:

1.

2. The insecticidal composition according to claim 1, characterized in that The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 20:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 24:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15~24:

1.

3. The insecticidal composition according to claim 1, characterized in that The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 25:1; The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 10:1; The active ingredient B is cyantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 16:1; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10~14:

1.

4. The insecticidal composition according to claim 1, characterized in that In addition to the active ingredients, the insecticide composition also includes other pesticide-acceptable adjuvants, which are selected from one or more of dispersants, wetting agents, emulsifiers, stabilizers, antifreeze agents, defoaming agents, preservatives, thickeners, solvents, organic acids, and dispersion media.

5. The insecticidal composition according to claim 1, characterized in that The composition is prepared into various dosage forms, including wettable powder, water-dispersible granules, suspension, aqueous emulsion, suspension seed coating, microcapsule suspension, emulsifiable concentrate, microemulsion, dispersible liquid or granule.

6. Use of the insecticidal composition according to any one of claims 1 to 5 for controlling agricultural or non-agricultural pests.

7. Use according to claim 6, characterized in that The pests are Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera and mites.

8. A method for controlling invertebrate pests, characterized in that: The method comprises contacting the invertebrate pest or its environment with a biologically effective amount of the composition of any one of claims 1 to 5 above.

9. A method for controlling insect pests in agriculture or non-agriculture, characterized in that: The method comprises applying the composition according to any one of claims 1 to 5 to plants where pests are present.

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