Preparation method of pet oral care agent

Abstract

The invention provides a preparation method of a pet oral care agent, and the pet oral care agent comprises the following components in parts by weight: 0.1-1 part of a surfactant, 2-4 parts of a sweetening agent, 6-8 parts of a stabilizer, 5-10 parts of a bacteriostatic agent, 0.4-0.6 part of protease, 0.5-1 part of hemicellulase and 60-80 parts of water. The preparation method comprises the following steps: S1, mixing and stirring the surfactant, the bacteriostatic agent, the protease and the hemicellulase according to the formula ratio, then adding deionized water, and heating and stirring to dissolve the components to obtain a mixture; and S2, adding the sweetening agent and the stabilizer into the mixture according to the formula ratio, and stirring to obtain the pet oral care agent. According to the preparation method of the pet oral care agent disclosed by the invention, the aspartic protease, the sulfydryl protease and the bacteriostatic agent have a synergistic effect in the aspect of bacteriostasis, and particularly have a remarkable bacteriostatic effect on common bacteria in the oral cavity of a pet.

Description

Technical Field

[0001] The invention relates to the technical field of pet antibacterial products, and in particular to a method for preparing a pet oral care agent. Background Art

[0002] The pet oral cleaning products on the market mainly include pet tooth cleaning water and pet toothpaste, both of which belong to the category of pet care products, rather than pet staple food or snacks. Pet oral cleaning products are usually eaten directly by pets after entering the mouth. Therefore, pet oral cleaning products must be non-toxic and meet the requirements of food safety, that is, the LD50 must at least reach the non-toxic level.

[0003] Currently on the market, pet feeds consumed by pets cannot be chewed or gnawed effectively by teeth. Meanwhile, long-term consumption of various additives can easily cause oral problems. However, existing pet oral care agents on the market have poor antibacterial properties and poor safety performance. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a method for preparing a pet oral care agent to improve the antibacterial effect and safety of the pet oral care agent.

[0005] The technical solution adopted by the present invention is:

[0006] A method for preparing a pet oral care agent, wherein: the pet oral care agent comprises the following components by weight: 0.1-1 parts of a surfactant, 2-4 parts of a sweetener, 6-8 parts of a stabilizer, 5-10 parts of an antibacterial agent, 0.4-0.6 parts of a protease, 0.5-1 parts of a hemicellulase, and 60-80 parts of water;

[0007] The preparation method comprises the following steps:

[0008] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, and obtain a mixture;

[0009] Step S2: adding a formulated amount of a sweetener and a stabilizer to the mixture and stirring the mixture to obtain a pet oral care agent.

[0010] Preferably, in the method for preparing the pet oral care agent, the surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sorbitan oleate, polysorbate, and glyceryl monostearate.

[0011] Preferably, in the method for preparing the pet oral care agent, the sweetener is selected from one or more of xylitol, steviol glycoside, sorbitol, maltitol and erythritol.

[0012] Preferably, in the method for preparing the pet oral care agent, the stabilizer comprises 2-4 parts of methyl benzoate, 1-3 parts of epoxidized soybean oil, and 1-3 parts of tridecyl ester.

[0013] Preferably, in the method for preparing the pet oral care agent, the antibacterial agent comprises 1-3 parts of ethyl vanillin, 2-4 parts of polyhexamethylene biguanide, and 2-3 parts of plant extracts.

[0014] Preferably, in the method for preparing the pet oral care agent, the plant extract is one or more of chamomile extract, tea extract, clove extract, lavender extract and rosemary extract.

[0015] Preferably, in the method for preparing the pet oral care agent, the protease comprises 0.2-0.3 parts of aspartic protease and 0.2-0.3 parts of thiol protease.

[0016] Preferably, in the method for preparing the pet oral care agent, the heating temperature in step S1 is 60-65°C.

[0017] Preferably, in the method for preparing the pet oral care agent, the stirring speed in step S1 and step S2 is 600 r / min to 800 r / min, and the stirring time is 30-60 min.

[0018] Advantages of the present invention:

[0019] The preparation method of the pet oral care agent of the present invention uses ethyl vanillin, polyhexamethylene biguanide and plant extracts to synergistically improve the antibacterial and antiseptic properties of the care agent; methyl benzoate, epoxy soybean oil and tridecyl ester are used synergistically as stabilizers to improve the long-term stability of the care agent; aspartic acid protease, thiol protease and antibacterial agent have a synergistic effect in antibacterial performance, especially the antibacterial effect on common bacteria in the pet's oral cavity is remarkable. DETAILED DESCRIPTION

[0020] The present invention will be further described below in conjunction with specific embodiments.

[0021] Example 1

[0022] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 0.1 part of a surfactant, 2 parts of a sweetener, 6 parts of a stabilizer, 5 parts of an antibacterial agent, 0.4 part of a protease, 0.5 part of a hemicellulase, and 80 parts of water; the surfactant is sodium dodecylbenzene sulfonate, the sweeteners are sorbitol and maltitol, and the mass ratio is 1:1; the stabilizer comprises 2 parts of methyl benzoate, 1 part of epoxy soybean oil, and 3 parts of tridecyl ester; the antibacterial agent comprises 1 part of ethyl vanillin, 2 parts of polyhexamethylene biguanide, and 2 parts of a plant extract; the plant extract is a chamomile extract and a tea extract, and the mass ratio is 1:1; the protease comprises 0.2 parts of aspartic protease and 0.2 parts of thiol protease;

[0023] The preparation method comprises the following steps:

[0024] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0025] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0026] Example 2

[0027] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 0.5 parts of a surfactant, 3 parts of a sweetener, 7 parts of a stabilizer, 8 parts of an antibacterial agent, 0.5 parts of a protease, 0.8 parts of a hemicellulase, and 80 parts of water; the surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sorbitan oleate, polysorbate, and glyceryl monostearate; the sweetener is selected from one or more of xylitol, steviol glycoside, sorbitol, maltitol, and erythritol; the stabilizer comprises 3 parts of methyl benzoate, 3 parts of epoxy soybean oil, and 1 part of tridecyl ester; the antibacterial agent comprises 2 parts of ethyl vanillin, 3 parts of polyhexamethylene biguanide, and 3 parts of a plant extract; the plant extract is a lavender extract; the protease comprises 0.2 parts of aspartic protease and 0.3 parts of thiol protease;

[0028] The preparation method comprises the following steps:

[0029] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0030] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0031] Example 3

[0032] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 1 part of a surfactant, 4 parts of a sweetener, 8 parts of a stabilizer, 10 parts of an antibacterial agent, 0.6 parts of a protease, 1 part of a hemicellulase, and 80 parts of water; the surfactant is glyceryl monostearate, the sweetener is erythritol, the stabilizer comprises 4 parts of methyl benzoate, 3 parts of epoxy soybean oil, and 1 part of tridecyl ester; the antibacterial agent comprises 3 parts of ethyl vanillin, 4 parts of polyhexamethylene biguanide, and 3 parts of a plant extract; the plant extract is a lavender extract and a rosemary extract, and the mass ratio is 1:1; the protease comprises 0.3 parts of aspartic protease and 0.3 parts of thiol protease;

[0033] The preparation method comprises the following steps:

[0034] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0035] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0036] Comparative Example 1

[0037] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 0.1 part of a surfactant, 2 parts of a sweetener, 6 parts of a stabilizer, 5 parts of an antibacterial agent, 0.4 parts of a protease, 0.5 parts of a hemicellulase, and 80 parts of water; the surfactant is sodium dodecylbenzene sulfonate, the sweetener is sorbitol and maltitol, and the mass ratio is 1:1; the stabilizer comprises 2 parts of methyl benzoate, 1 part of epoxy soybean oil, and 3 parts of tridecyl ester; the antibacterial agent is ethyl vanillin; the protease comprises 0.2 parts of aspartic protease and 0.2 parts of thiol protease;

[0038] The preparation method comprises the following steps:

[0039] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0040] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0041] Comparative Example 2

[0042] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 0.5 parts of a surfactant, 3 parts of a sweetener, 7 parts of a stabilizer, 8 parts of an antibacterial agent, 0.5 parts of a protease, 0.8 parts of a hemicellulase, and 80 parts of water; the surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sorbitan oleate, polysorbate, and glyceryl monostearate; the sweetener is selected from one or more of xylitol, steviol glycoside, sorbitol, maltitol, and erythritol; the stabilizer comprises 3 parts of methyl benzoate, 3 parts of epoxy soybean oil, and 1 part of tridecyl ester; the antibacterial agent comprises 5 parts of polyhexamethylene biguanide and 3 parts of a plant extract; the plant extract is a lavender extract; the protease comprises 0.2 parts of aspartic protease and 0.3 parts of thiol protease;

[0043] The preparation method comprises the following steps:

[0044] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0045] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0046] Comparative Example 3

[0047] A method for preparing a pet oral care agent, wherein the pet oral care agent comprises the following components by weight: 1 part of a surfactant, 4 parts of a sweetener, 8 parts of a stabilizer, 10 parts of an antibacterial agent, 1 part of a hemicellulase, and 80 parts of water; the surfactant is glyceryl monostearate, the sweetener is erythritol, the stabilizer comprises 4 parts of methyl benzoate, 3 parts of epoxy soybean oil, and 1 part of tridecyl ester; the antibacterial agent comprises 3 parts of ethyl vanillin, 4 parts of polyhexamethylene biguanide, and 3 parts of a plant extract; the plant extract is a lavender extract and a rosemary extract, and the mass ratio is 1:1;

[0048] The preparation method comprises the following steps:

[0049] Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, the heating temperature is 60°C, the stirring speed is 700r / min, and the stirring time is 30min to obtain a mixture;

[0050] Step S2. Add the formulated amount of sweetener and stabilizer to the mixture and stir at a stirring speed of 700 r / min for 30 min to obtain a pet oral care agent.

[0051] The pet oral care agents of Examples 1-3 and Comparative Examples 1-3 were tested for antibacterial rate, and the test results are shown in Table 1.

[0052] Table 1

[0053] Pasteurella Streptococcus staphylococcus Candida albicans Example 1 99.8％ 99.6％ 99.1％ 99.3％ Example 2 99.8％ 99.2％ 99.4％ 99.2％ Example 3 99.1％ 99.3％ 99.9％ 99.5％ Comparative Example 1 56.1％ 49.2％ 89.4％ 67.4％ Comparative Example 2 67.5％ 62.1％ 78.4％ 81.6％ Comparative Example 3 45.4％ 85.1％ 65.8％ 88.3％

[0054] The results in Table 1 show that ethyl vanillin, polyhexamethylene biguanide and plant extracts work synergistically, and the antibacterial effect is significantly improved compared with using them alone. In addition, the test action time is 5 minutes, and the antibacterial performance can reach more than 99% within 5 minutes, indicating that rapid sterilization can be achieved, and the growth of microorganisms can be inhibited and killed in a short time.

[0055] Finally, it should be noted that the above specific implementation methods are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to examples, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for preparing a pet oral care agent, characterized in that: The pet oral care agent includes the following components by weight: 0.1-1 parts of surfactant, 2-4 parts of sweetener, 6-8 parts of stabilizer, 5-10 parts of antibacterial agent, 0.4-0.6 parts of protease, 0.5-1 parts of hemicellulase, and 60-80 parts of water; The preparation method comprises the following steps: Step S1. Take the formulated amount of surfactant, antibacterial agent, protease, and hemicellulase, mix and stir, then add deionized water, heat and stir to dissolve, and obtain a mixture; Step S2: adding a formulated amount of a sweetener and a stabilizer to the mixture and stirring the mixture to obtain a pet oral care agent.

2. The method for preparing the pet oral care agent according to claim 1, characterized in that: The surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sorbitan oleate, polysorbate, and glyceryl monostearate.

3. The method for preparing the pet oral care agent according to claim 1, characterized in that: The sweetener is selected from one or more of xylitol, steviol glycoside, sorbitol, maltitol and erythritol.

4. The method for preparing the pet oral care agent according to claim 1, characterized in that: The stabilizer comprises 2-4 parts of methyl benzoate, 1-3 parts of epoxidized soybean oil, and 1-3 parts of tridecyl ester.

5. The method for preparing the pet oral care agent according to claim 1, characterized in that: The antibacterial agent comprises 1-3 parts of ethyl vanillin, 2-4 parts of polyhexamethylene biguanide and 2-3 parts of plant extract.

6. The method for preparing the pet oral care agent according to claim 5, characterized in that: The plant extract is one or more of chamomile extract, tea extract, clove extract, lavender extract and rosemary extract.

7. The method for preparing the pet oral care agent according to claim 1, characterized in that: The protease comprises 0.2-0.3 parts of aspartic protease and 0.2-0.3 parts of thiol protease.

8. The method for preparing the pet oral care agent according to claim 1, characterized in that: The heating temperature in step S1 is 60-65°C.

9. The method for preparing the pet oral care agent according to claim 1, characterized in that: In step S1 and step S2, the stirring speed is 600 r / min to 800 r / min, and the stirring time is 30 to 60 min.