Application of bacterial cellulose loaded with nano cerium oxide particles in preparation of medicine for treating inflammatory bowel disease

Abstract

The invention belongs to the field of biomedicine and material engineering, and particularly relates to application of bacterial cellulose loaded with nano cerium oxide particles in preparation of drugs for treating inflammatory bowel diseases. The novel medicine for treating ulcerative colitis is formed by compounding the nano cerium oxide particles and the bacterial cellulose, has the advantage of improving the stability of the bacterial cellulose in gastrointestinal tracts, plays the anti-oxidation and anti-inflammatory effects of the cerium oxide particles at the same time, promotes tissue repair and regeneration, and can be used for treating ulcerative colitis. The cerium oxide nano-particle-loaded bacterial cellulose drug has the advantages that the cerium oxide nano-particle-loaded bacterial cellulose drug is used as a carrier and synergistically exerts a treatment effect with the bacterial cellulose, a new possibility is provided for treatment of ulcerative colitis, and the cerium oxide nano-particle-loaded bacterial cellulose drug can realize targeted treatment of ulcerative colitis focuses and can directly reach diseased regions through oral administration, so that the treatment effect is greatly improved, and the treatment cost is reduced. Compared with the traditional UC treatment medicine, the medicine disclosed by the invention can reduce the distribution range in vivo, so that the risk of side effects of the system is reduced.

Description

Technical Field

[0001] The invention belongs to the field of biomedicine and material engineering, and specifically relates to the application of bacterial cellulose loaded with nano-cerium oxide particles in the preparation of medicines for treating inflammatory bowel disease. Background Art

[0002] Ulcerative colitis (UC) is a chronic inflammatory bowel disease that primarily affects the colonic mucosa. UC can occur at any age, and its typical symptoms include abdominal pain, diarrhea, malnutrition, bloody stools, anemia, etc. Patients often experience periodic attacks and remissions. The etiology and pathogenesis of UC are not yet fully understood, but there is a lot of evidence that the interaction between the patient's genotype, immune system, and intestinal microbiota plays a vital role in the pathogenesis of UC. The characteristic manifestation of the disease is continuous inflammation on the colonic mucosa, which usually starts in the rectum and extends upward to other parts of the colon, increasing the risk of colon cancer. In recent years, the incidence of UC has continued to increase, and patients often face a serious decline in quality of life. The complexity and chronicity of the disease have brought long-term pain to patients.

[0003] Treating ulcerative colitis is a challenging task. The main purpose of current treatment is to quickly induce remission during the active phase of the disease and maintain remission for a long time, while preventing the occurrence of complications. When UC cannot be cured, we should actively seek treatments to reduce intestinal inflammation, reduce the risk of complications and cancer, and try to avoid surgery. Current treatments include drug therapy, surgical intervention, and lifestyle adjustments, among which traditional UC drugs are still the main means of clinical treatment. However, existing treatments have some limitations, including drug side effects, limited efficacy, and surgical risks. Therefore, it is particularly important to seek innovative treatments to improve treatment effects, reduce side effects, and improve patients' quality of life. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art and to provide a bacterial cellulose loaded with nano-cerium oxide particles for use in the preparation of a drug for preventing and / or treating inflammatory bowel disease. By compounding nano-cerium oxide particles with bacterial cellulose, a new composite material for treating ulcerative colitis is formed to improve the stability of bacterial cellulose in the gastrointestinal tract, while exerting the antioxidant and anti-inflammatory effects of cerium dioxide, thereby providing new possibilities for the treatment of ulcerative colitis.

[0005] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0006] In a first aspect, the present invention provides the use of bacterial cellulose loaded with nano-cerium oxide particles in the preparation of a drug for preventing and / or treating inflammatory bowel disease.

[0007] Nano-cerium oxide particles (CeO2NPs) are an important oxide nanomaterial. Ce(III) and Ce(IV) on their surface can be automatically converted and regenerated, thereby scavenging free radicals and reducing oxidative damage to exert excellent antioxidant properties. CeO2NPs also have anti-inflammatory properties and can inhibit the release of inflammatory factors and the occurrence of inflammatory responses. In addition, CeO2NPs also have antibacterial properties, promote wound healing and promote tissue regeneration.

[0008] Bacterial cellulose is a natural polysaccharide polymer synthesized by bacteria. It has a linear polymer structure composed of many glucose molecules connected by β-1,4-glycosidic bonds. This structure gives bacterial cellulose excellent physical and chemical properties, including excellent mechanical strength, heat resistance, solvent resistance, biocompatibility and biodegradability. Therefore, it can be used as a drug delivery carrier to help control the release rate of drugs and improve the bioavailability of drugs. It can also be used in tissue engineering as a scaffold material for cell growth and tissue regeneration. It can also be used as a biodegradable wound dressing to promote wound healing and reduce the occurrence of infection and inflammation.

[0009] The present invention prepares a novel drug for treating inflammatory bowel disease by preparing nano cerium oxide particles and loading them on bacterial cellulose through a microwave synthesis method. The drug can exert a targeted therapeutic effect and can directly reach the lesion site through oral administration; and the two cooperate with each other to effectively improve the stability of bacterial cellulose in the gastrointestinal tract, while exerting the antioxidant and anti-inflammatory effects of cerium dioxide, promoting tissue repair and regeneration, and achieving a synergistic therapeutic effect with bacterial cellulose; and the drug has low toxicity and good biocompatibility, is not easy to cause immune response and toxic side effects, helps to reduce side effects and risks in the treatment process, and provides new possibilities for the treatment of inflammatory bowel disease.

[0010] Preferably, the inflammatory bowel disease is ulcerative colitis.

[0011] Preferably, the method for preparing the bacterial cellulose loaded with nano-cerium oxide particles comprises the following steps: mixing cerium nitrate and bacterial cellulose in water, adjusting the pH value to 10-12, and then subjecting the mixture to microwave treatment to obtain the bacterial cellulose loaded with nano-cerium oxide particles.

[0012] Preferably, the mass ratio of cerium nitrate to bacterial cellulose is (15-20):1.

[0013] The inventors have found through experimental research that the dosage ratio of cerium nitrate and bacterial cellulose has a significant effect on the therapeutic effect of ulcerative colitis of the bacterial cellulose drug loaded with nano-cerium oxide particles. The appropriate dosage ratio can ensure that the cerium oxide particles are effectively loaded on the bacterial cellulose matrix, thereby improving the activity and stability of the drug, reducing the impact of gastric acid and gastric juice on bacterial cellulose, and synergistically enhancing the therapeutic effect of ulcerative colitis; and the dosage ratio of the two will affect the biocompatibility of the drug to a certain extent. The appropriate dosage ratio can ensure that the drug has low toxicity to the organism during the treatment process and help to improve the biodegradability of the drug. When the mass ratio of cerium nitrate to bacterial cellulose is within the above-mentioned preferred range, the bacterial cellulose drug loaded with nano-cerium oxide particles can exert a better therapeutic effect on ulcerative colitis, and has low toxicity and good biocompatibility, is not easy to cause immune response and toxic side effects, helps to reduce side effects and risks during the treatment process, and improves the safety and controllability of the treatment.

[0014] Preferably, the specific conditions of the microwave treatment are: reaction temperature 100-140° C., reaction time 4-6 min, heating time 2-4 min, stirring speed 350-450 rpm.

[0015] The present invention loads nano-cerium oxide particles on bacterial cellulose through microwave reaction, wherein parameters such as reaction temperature and reaction time are equally important to the particle size, loading efficiency and other properties of the prepared drug. Experimental investigations have found that when microwave treatment is carried out under the parameter conditions within the above-mentioned preferred range, nano-cerium oxide particles can be better loaded on bacterial cellulose, which helps to produce smaller and evenly dispersed particles, thereby exerting a better effect in treating ulcerative colitis.

[0016] Preferably, the pH value of the solution is adjusted to 10-12 using NH4OH.

[0017] In a second aspect, the present invention provides a method for preparing bacterial cellulose loaded with nano-cerium oxide particles, comprising the following steps: mixing cerium nitrate and bacterial cellulose in water, adjusting the pH value to 10-12, and then subjecting the mixture to microwave treatment to obtain the bacterial cellulose loaded with nano-cerium oxide particles; the mass ratio of cerium nitrate to bacterial cellulose is (15-20):1.

[0018] Preferably, the specific conditions of the microwave treatment are: reaction temperature 100-140° C., reaction time 4-6 min, heating time 2-4 min, stirring speed 350-450 rpm.

[0019] In a third aspect, the present invention provides a drug for treating ulcerative colitis, the drug comprising bacterial cellulose loaded with nano-cerium oxide particles prepared by the above-mentioned preparation method.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The present invention forms a novel drug for treating ulcerative colitis by combining nano-cerium oxide particles with bacterial cellulose. The drug has the advantage of improving the stability of bacterial cellulose in the gastrointestinal tract, while exerting the antioxidant and anti-inflammatory effects of cerium dioxide, promoting tissue repair and regeneration, and synergistically exerting therapeutic effects with bacterial cellulose, thereby providing new possibilities for the treatment of ulcerative colitis.

[0022] (2) The bacterial cellulose drug loaded with nano-cerium oxide particles described in the present invention can achieve targeted treatment of ulcerative colitis lesions. The drug can be directly delivered to the lesion site through oral administration. The carrier effect of bacterial cellulose and the anti-inflammatory effect of cerium dioxide nanoparticles can more accurately treat ulcerative colitis. In addition, compared with traditional UC drug treatments, the drug described in the present invention can reduce the distribution range in the body, thereby reducing the risk of systemic side effects.

[0023] (3) The bacterial cellulose drug loaded with nano-cerium oxide particles described in the present invention has low toxicity and good biocompatibility, is not easy to cause immune response and toxic side effects, helps to reduce side effects and risks during treatment, and improves the safety and controllability of treatment. In addition, combining nano-cerium oxide particles with bacterial cellulose can improve the stability of bacterial cellulose in the gastrointestinal tract and reduce its influence by gastric acid and gastric juice, thereby improving the treatment effect and controllability. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The disease activity index graphs of mice in the control group, model group, and treatment group;

[0025] Figure 2 Figure 2 is the weight change of mice in the control group, model group and treatment group;

[0026] Figure 3 This is a graph showing the contents of IL-6, IL-1β, and TNF-α in mouse colon tissue homogenate;

[0027] Figure 4 Figure 2 is the HE staining of the colon tissue of mice in the control group, model group and treatment group (Figure A), and Figure B is the HE staining of the colon tissue of mice in the control group, model group and treatment group (Figure A). Figure 3 A picture is randomly selected from the group and enlarged 20 times;

[0028] Figure 5 This is the TEM image of the excreta after mice consumed BC alone;

[0029] Figure 6This is a TEM image of the excrement of mice after eating BC@CeO2NPs prepared in Example 2;

[0030] Figure 7 Figure 1 shows the TEM image (Figure A) and diffraction pattern (Figure B) of the excrement after mice consumed CeO2NPs alone. DETAILED DESCRIPTION

[0031] To better illustrate the content of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0032] Unless otherwise specified, the reagents used in the examples are conventional reagents in the art and can be purchased through commercial channels. The experimental operations not specifically described in the examples are conventional operations in the art or can be understood or known by those skilled in the art based on the existing technology or common knowledge they master.

[0033] Example 1

[0034] This embodiment provides a method for preparing bacterial cellulose (BC), comprising the following steps:

[0035] (1) Culture medium preparation

[0036] ① Preparation of liquid culture medium (taking 1000 mL as an example): In 1000 mL of ultrapure water, add 20 g of glucose, 5 g of yeast extract powder, 5 g of peptone, 1.15 g of anhydrous citric acid and 6.8 g of disodium hydrogen phosphate dodecahydrate, autoclave at 120°C for 30 min before use;

[0037] ② Preparation of solid culture medium (taking 1000 mL as an example): In 1000 mL of ultrapure water, add 20 g of glucose, 5 g of yeast extract powder, 5 g of peptone, 1.15 g of anhydrous citric acid, 6.8 g of disodium hydrogen phosphate dodecahydrate and 15 g of agar in sequence, sterilize at 120°C for 30 min, and prepare agar plates;

[0038] (2) Strain activation and BC culture

[0039] Take out a tube of frozen Acetobacter xylinum from a -80℃ refrigerator, and put it into -20℃ and -4℃ refrigerators for thawing. Then use a disposable inoculation loop to take out the bacteria from the tube and draw a "Z" on the solid agar plate until the whole plate is drawn. Then make a mark on the plate, and still put the plate upside down in the incubator for about 3 days. After three days, take 3mL of liquid culture medium and add it to a 15mL centrifuge tube. Use an inoculation loop to scrape a colony and add it to 3mL of liquid culture medium, and then transfer it to a constant temperature centrifuge tube. In the incubator, culture at a constant temperature of 26°C for 3 days; three days later, take 40mL of liquid culture medium and add it to a 50mL centrifuge tube, then transfer 2mL of the bacterial liquid in the 15mL centrifuge tube to the centrifuge tube, transfer it to the incubator, and culture it at 26°C for 3 days; three days later, add 200mL of the aforementioned liquid culture medium to a 1L container bottle, then transfer the bacterial liquid in the 50mL centrifuge tube to the container, move it to the incubator and culture it at 26°C for 3 days; three days later, a white milky film can be observed, and BC is obtained.

[0040] (3) Cleaning of BC

[0041] After the culture is completed, the BC is taken out and immersed in an ethanol solution for sterilization, and then continuously heated and stirred with ultrapure water at 95°C for 30 minutes, and then washed with 0.5wt% NaOH solution at 95-98°C for 20 minutes, and repeated 2-3 times to remove the culture medium and bacteria on the film until the BC film becomes transparent. Finally, it is washed with ultrapure water at 97-98°C for 40 minutes to wash away the alkali solution in the film, and the solution is tested with pH paper until it is neutral, thereby obtaining the bacterial cellulose (BC).

[0042] Example 2

[0043] This embodiment provides a method for preparing bacterial cellulose loaded with nano-cerium oxide particles (BC@CeO2 NPs), comprising the following steps:

[0044] Take 98.65mL of ultrapure water, add 434mg of Ce(NO3)3, stir thoroughly to dissolve, then add 1.35mL of 1M NH4OH solution to the Ce(NO3)3 solution, shake quickly, adjust the pH value to 11, then add 20mL of the solution to each microwave tube, put in 5.05mg BC for microwave reaction, and prepare bacterial cellulose loaded with nano-cerium oxide particles; wherein, the microwave reaction conditions are as follows: heating time 3min, reaction temperature 120℃, reaction time 5min, stirring speed 400rpm.

[0045] Example 3

[0046] This embodiment provides a method for preparing bacterial cellulose loaded with nano-cerium oxide particles (BC@CeO2 NPs), comprising the following steps:

[0047] Take 98.65mL of ultrapure water, add 378mg of Ce(NO3)3, stir thoroughly to dissolve, then add 1.35mL of 1M NH4OH solution to the Ce(NO3)3 solution, shake quickly, adjust the pH value to 11, then add 20mL of the solution to each microwave tube, put in 5.05mgBC for microwave reaction, and prepare bacterial cellulose loaded with nano-cerium oxide particles; wherein, the microwave reaction conditions are as follows: heating time 2min, reaction temperature 100℃, reaction time 6min, stirring speed 450rpm.

[0048] Example 4

[0049] This embodiment provides a method for preparing bacterial cellulose loaded with nano-cerium oxide particles (BC@CeO2 NPs), comprising the following steps:

[0050] Take 98.65mL of ultrapure water, add 505mg of Ce(NO3)3, stir thoroughly to dissolve, then add 1.35mL of 1M NH4OH solution to the Ce(NO3)3 solution, shake quickly, adjust the pH value to 11, then add 20mL of the solution to each microwave tube, put in 5.05mgBC for microwave reaction, and prepare bacterial cellulose loaded with nano-cerium oxide particles; wherein, the microwave reaction conditions are as follows: heating time 4min, reaction temperature 140℃, reaction time 4min, stirring speed 350rpm.

[0051] Comparative Example 1

[0052] The difference between this comparative example and Example 2 is that the added amount of Ce(NO3)3 is 252.5 mg.

[0053] Comparative Example 2

[0054] The difference between this comparative example and Example 2 is that the added amount of Ce(NO3)3 is 631.5 mg.

[0055] Comparative Example 3

[0056] The difference between this comparative example and Example 2 is that the added amount of bacterial cellulose (BC) is 86.8 mg.

[0057] Comparative Example 4

[0058] The difference between this comparative example and Example 2 is that the reaction temperature during the microwave reaction is 90° C. and the reaction time is 8 min.

[0059] Comparative Example 5

[0060] The difference between this comparative example and Example 2 is that the reaction temperature during the microwave reaction is 150° C. and the reaction time is 2 min.

[0061] Comparative Example 6

[0062] The difference between this comparative example and Example 2 is that the stirring speed during the microwave reaction is 300 rpm.

[0063] Comparative Example 7

[0064] The difference between this comparative example and Example 2 is that the stirring speed during the microwave reaction is 500 rpm.

[0065] Effect Example 1

[0066] In this effect example, the bacterial cellulose loaded with nano-cerium oxide particles prepared in Examples 2-4 and Comparative Examples 1-7 was used as samples, and the particle size of the nano-cerium oxide particles (CeO2 NPs) was tested by transmission electron microscopy. The results are shown in Table 1.

[0067] Table 1

[0068]

[0069] The results in Table 1 show that in the bacterial cellulose loaded with nano-cerium oxide particles prepared by Examples 2-4, the CeO2NPs have stable performance, uniform and relatively small size, which can ensure that the bacterial cellulose is more uniformly and controllably loaded, thereby improving the activity and stability of the drug, and reducing the effects of gastric acid and gastric juice on the bacterial cellulose, which is helpful to improve the therapeutic effect. However, when the dosage ratio of cerium nitrate to bacterial cellulose and the reaction conditions involved in the preparation process in Comparative Examples 1-7 are outside the preferred range of the present invention, the size of the prepared CeO2NPs is relatively larger and the particle size distribution is uneven, which will affect the effective loading of the generated CeO2NPs on the bacterial cellulose, resulting in reduced stability and anti-inflammatory activities of the bacterial cellulose drug loaded with nano-cerium oxide particles, thereby affecting the therapeutic effect of the drug.

[0070] Effect Example 2

[0071] In this effect example, the bacterial cellulose loaded with nano-cerium oxide particles prepared in Example 2 was used as a sample, and was orally administered to mice with colitis induced by dextran sulfate sodium (DSS) to explore its therapeutic effect on ulcerative colitis. The specific method is as follows.

[0072] (1) Grouping

[0073] Table 2

[0074]

[0075] (2) Modeling

[0076] DSS was prepared into a 3% aqueous solution and loaded into a 50 ml centrifuge tube. The centrifuge tube was placed in the water bottle of the mice (model group, treatment group) and sealed tightly. A new DSS aqueous solution was replaced every 2 days until the seventh day of drinking, when the modeling was completed, and then a 1.5% DSS aqueous solution was replaced until the end of the experiment; for the treatment group, mice were gavaged daily according to the drug dose. During the administration period, the weight, diet, and general mental health of the mice in the control group, model group, and treatment group were observed weekly, and the stool characteristics were observed and recorded, and scored according to the DAI scoring standard in Table 3. Disease Activity Index (DAI) = (body mass index + stool shape + blood in stool) / 3.

[0077] Table 3

[0078]

[0079]

[0080] (3) Results and analysis

[0081] like Figure 1 As shown, compared with the model group, the mice in the treatment group that took the bacterial cellulose drug loaded with nano-cerium oxide particles prepared by the present invention had a significantly lower disease activity index than the model group after the treatment cycle, indicating that the bacterial cellulose drug loaded with nano-cerium oxide particles of the present invention has a good therapeutic effect on DSS-induced ulcerative colitis, has low toxicity and good biocompatibility, is not easy to cause immune response and toxic side effects in the organism, and can effectively improve the physical condition of patients with ulcerative colon cancer; Figure 2 The results also show that taking the bacterial cellulose drug loaded with nano-cerium oxide particles described in the present invention can effectively maintain the weight of mice. The nano-cerium oxide particles in the drug have certain antibacterial properties. In the process of treating colitis, the drug can not only directly intervene in the infectious factors of the disease through antibacterial effects, but also retain probiotics, thereby achieving balanced regulation of the intestinal microecology. Therefore, the bacterial cellulose drug loaded with nano-cerium oxide particles described in the present invention can not only reduce the degree of intestinal inflammation and achieve better therapeutic effects, but also the drug can be used in related products such as health care and weight loss to effectively maintain a good and stable physical condition.

[0082] The present invention also performed ELISA detection on mouse tissue homogenate. After the administration period of mice, the mice were fasted for 12 hours, and the connective tissues of the mice were taken to detect the contents of IL-6, IL-1β, and TNF-α in the tissue homogenate. The results were as follows: Figure 3As shown in the figure, the contents of IL-6, IL-1β and TNF-α pro-inflammatory cytokines in the tissues of the mice in the treatment group were lower than those in the model group that did not take the drug of the present invention; the pathological changes of the colon tissue observed by HE staining also showed that the intestinal tissue of the control group was relatively healthy, while the mice in the model group induced by DSS had severe induced inflammatory infiltration and epithelial tissue damage, while the severity of the mice in the treatment group that took the bacterial cellulose drug loaded with nano-cerium oxide particles of the present invention was significantly lower than that in the model group ( Figure 4 ); The above results all indicate that the bacterial cellulose loaded with nano-cerium oxide particles of the present invention, as a new type of drug, has excellent antioxidant and anti-inflammatory effects, and can achieve targeted treatment of ulcerative colitis lesions, promote tissue repair and regeneration, exert good therapeutic effects, and provide new possibilities for the treatment of ulcerative colitis.

[0083] In addition, the present invention also explored the therapeutic effect of using CeO2NPs alone, and found that when CeO2NPs entered the body from the mouse mouth, CeO2NPs gradually dissolved or agglomerated, and the stability was significantly reduced, resulting in a significant weakening of its therapeutic effect. CeO2NPs basically could not play a big role when they reached the mouse intestine. After using bacterial cellulose to load CeO2NPs, its performance was stable, and it was basically on the bacterial cellulose without detachment, which effectively ensured the stability and efficiency of CeO2NPs.

[0084] Figure 5 This is a TEM image of the excrement of mice fed with bacterial cellulose BC alone. In the image, only cellulose of BC is seen without any nanoparticles. Figure 6 This is a TEM image of the excrement of mice after eating the BC@CeO2NPs prepared in Example 2. The image shows that the CeO2NPs nanoparticles loaded on the BC cellulose can be clearly seen in the excrement after cleaning. Figure 7 The TEM image (Figure A) and diffraction pattern (Figure B) of the excrement of mice after feeding them with CeO2 NPs alone show that no nanoparticles were found in the excrement, and they were basically dissolved or agglomerated. The above results all indicate that the present invention uses bacterial cellulose to load nano-cerium oxide particles, which can effectively improve the stability of the nanoparticles in the gastrointestinal tract and achieve targeted treatment of ulcerative colitis lesions. The two work together to synergistically exert a therapeutic effect, providing new possibilities for the treatment of ulcerative colitis.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. Application of bacterial cellulose loaded with nano-cerium oxide particles in the preparation of drugs for preventing and / or treating inflammatory bowel disease.

2. The use according to claim 1, characterized in that: The inflammatory bowel disease is ulcerative colitis.

3. The use according to claim 1, characterized in that: The preparation method of the bacterial cellulose loaded with nano-cerium oxide particles comprises the following steps: mixing cerium nitrate and bacterial cellulose in water, adjusting the pH value to 10-12, and then performing microwave treatment to obtain the bacterial cellulose loaded with nano-cerium oxide particles.

4. The use according to claim 3, characterized in that: The mass ratio of the cerium nitrate to the bacterial cellulose is (15-20):

1.

5. The use according to claim 3, characterized in that: The specific conditions of the microwave treatment are: reaction temperature 100-140° C., reaction time 4-6 min, heating time 2-4 min, stirring speed 350-450 rpm.

6. The use according to claim 3, characterized in that: The pH of the solution was adjusted to 10-12 using NH4OH.

7. A method for preparing bacterial cellulose loaded with nano-cerium oxide particles, characterized in that: The method comprises the following steps: mixing cerium nitrate and bacterial cellulose in water, adjusting the pH value to 10-12, and then subjecting to microwave treatment to obtain the bacterial cellulose loaded with nano-cerium oxide particles; The mass ratio of the cerium nitrate to the bacterial cellulose is (15-20):

1.

8. The preparation method according to claim 7, characterized in that: The specific conditions of the microwave treatment are: reaction temperature 100-140° C., reaction time 4-6 min, heating time 2-4 min, and stirring speed 350-450 rpm.

9. A drug for treating ulcerative colitis, characterized in that: The drug comprises bacterial cellulose loaded with nano-cerium oxide particles prepared by the preparation method according to any one of claims 7 or 8.

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