Traditional Chinese medicine compound for treating sarcopenia and application thereof

Abstract

The invention provides a traditional Chinese medicine compound for treating sarcopenia, which is prepared from the following components in parts by mass: 20 to 60 parts of fructus lycii, 20 to 60 parts of salted semen cuscutae, 10 to 30 parts of fructus rubi, 5 to 15 parts of fried semen plantaginis and 5 to 15 parts of fructus schizandrae. The traditional Chinese medicine compound can effectively treat sarcopenia.

Description

Technical Field

[0001] The invention relates to a traditional Chinese medicine compound for treating sarcopenia and application thereof, belonging to the technical field of traditional Chinese medicines. Background Art

[0002] Sarcopenia, a health problem that becomes more and more serious with age, is characterized by a significant reduction in muscle mass, weakened muscle strength, or overall degeneration of muscle physiological function. This disease is progressive and not only affects the patient's daily life, but also greatly increases the risk of adverse consequences such as disability, fractures, frequent falls, and even life-threatening conditions. As a chronic musculoskeletal disease, the incidence of sarcopenia is rising year by year, bringing unprecedented challenges and burdens to the social health system.

[0003] Looking back at ancient Chinese medical books, we have not been able to directly find the exact term "sarcopenia". However, in recent years, Chinese medical researchers, relying on the profound theoretical foundation of Chinese medicine and rich clinical practice, have classified this modern disease into the category of "meat atrophy" in Chinese medicine. They believe that the fundamental pathogenesis of sarcopenia lies in the weakness of spleen and stomach function and the deficiency of liver and kidney qi. However, despite such theoretical understanding, under current technical conditions, we have not yet found a Chinese medicine compound that can comprehensively and effectively treat sarcopenia. This is both a challenge and an important direction that needs to be explored in depth in future research on Chinese medicine. Summary of the invention

[0004] The present invention provides a traditional Chinese medicine compound for treating sarcopenia and application thereof, which can effectively solve the above problems.

[0005] The present invention is achieved in that:

[0006] A traditional Chinese medicine compound for treating sarcopenia comprises the following components by weight: 20-60 parts of wolfberry fruit, 20-60 parts of salt dodder seed, 10-30 parts of raspberry, 5-15 parts of stir-fried plantain seed and 5-15 parts of schisandra chinensis.

[0007] In some embodiments, the above-mentioned Chinese herbal compound for treating sarcopenia comprises the following components by mass: 30-50 parts of wolfberry, 30-50 parts of salt dodder seeds, 15-25 parts of raspberry, 8-12 parts of stir-fried plantain seeds and 8-12 parts of schisandra chinensis.

[0008] In some embodiments, the above-mentioned Chinese herbal compound for treating sarcopenia includes the following components by mass: 40 parts of wolfberry, 40 parts of salt dodder seeds, 20 parts of raspberry, 10 parts of stir-fried plantain seeds and 10 parts of schisandra chinensis.

[0009] A preparation method of a Chinese medicine compound for treating sarcopenia comprises the following steps: firstly crushing the above Chinese medicines, placing them in a decoction container, soaking them in distilled water 6-10 times the mass of the Chinese medicines, then heating them, boiling them, decocting them again, filtering them, and collecting the filtrate; adding 6-10 times the distilled water to the residues to continue decocting, boiling them, and filtering them; combining the two decoctions, concentrating them in a hot water bath; cooling them at room temperature, packaging them, and refrigerating them for later use.

[0010] In some embodiments, the crushed particle size is 50-100 mesh.

[0011] In some embodiments, the soaking time is 25-35 minutes.

[0012] In some embodiments, the decocting time is 0.8-1.5h.

[0013] In some embodiments, the concentration is to concentrate to 2.5-3.0 g of medicinal material per 1 ml of the medicinal solution.

[0014] A use of the above-mentioned traditional Chinese medicine compound in preparing a medicine for treating sarcopenia.

[0015] In some embodiments, the drug for treating sarcopenia is in the form of a decoction, capsule, tablet, granule, powder or pill.

[0016] The beneficial effects of the present invention are:

[0017] The Chinese medicine compound of the present invention is composed of wolfberry, dodder, schisandra, plantain and raspberry, wherein wolfberry can balance yin and yang, nourish the kidney and replenish essence, and is the main medicine; dodder can both nourish yin and support yang, warm without drying, nourish without stagnation, and is the ministerial medicine; raspberry and schisandra nourish yin and strengthen the kidney, astringe essence and stop nocturnal emission, and are adjuvant medicines; plantain is diuretic and relieves the deficiency fire in the kidney, and is the guiding medicine. All the medicines cooperate to play the effect of nourishing the kidney and replenishing essence, and the Chinese medicine compound is quite effective in treating sarcopenia. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a bar graph of the grip test of Example 2 of the present invention.

[0020] Figure 2 This is a statistical chart of lower limb lean mass / body weight in Example 2 of the present invention.

[0021] Figure 3 This is a statistical diagram of the cross-sectional area of ​​muscle fibers in Example 2 of the present invention.

[0022] Figure 4 This is a statistical chart of the grip test of Example 3 of the present invention.

[0023] Figure 5 This is a statistical chart of lower limb lean mass / body weight in Example 3 of the present invention.

[0024] Figure 6 This is a statistical diagram of the cross-sectional area of ​​muscle fibers in Example 3 of the present invention.

[0025] Figure 7 HE staining images of muscles of each group in Example 3 of the present invention. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0027] A Chinese herbal compound preparation for treating sarcopenia, wherein the ingredients of the formula are accurately measured according to the mass ratio, and specifically include the following main ingredients: 20 to 60 parts of wolfberry, 20 to 60 parts of salt dodder seeds, 10 to 30 parts of raspberry, 5 to 15 parts of stir-fried plantain seeds and 5 to 15 parts of schisandra chinensis.

[0028] The medicinal properties and effects of each raw material are described as follows:

[0029] Salt Cuscuta australis: spicy and sweet in taste, warm in nature, enters the liver and kidney meridians, and its main functions and effects: tonifies yang and benefits yin, removes wind and improves eyesight, stops diarrhea and consolidates essence, nourishes muscles and strengthens yin, strengthens tendons and bones, replenishes deficiencies, and benefits qi and strength. It can both benefit yin and support yang, is warm without being dry, and replenishes without being stagnant. It is mainly used to treat soreness of the waist and knees, impotence and spermatorrhea, frequent urination, and excessive leucorrhea. It can also treat symptoms such as blurred vision caused by liver and kidney deficiency, and poor appetite, loose stools and diarrhea caused by spleen deficiency.

[0030] Lycium barbarum: sweet in taste, neutral in nature, enters the liver and kidney meridians, and its main functions and effects: nourishes the liver and kidney, benefits the essence and blood, improves eyesight, nourishes yin and moistens the lungs, can balance yin and yang, benefit the kidney and replenish essence, and is mainly used to treat dizziness, decreased vision, weakness in the waist and knees, spermatorrhea, and thirst caused by liver and kidney yin deficiency, and can also treat yin deficiency and fatigue cough.

[0031] Schisandra chinensis: sour in taste, warm in nature, enters the lung, kidney and heart meridians. Its main functions and indications: astringes the lungs and nourishes the kidneys, promotes fluid production and astringes sweat, astringes the semen and stops diarrhea, and calms the mind and soothes the nerves. The sourness of this product can be astringent, and it is warm and moist in nature. It can astringe the lung qi upwards, nourish the kidney yin downwards, nourish yin and consolidate the kidneys, astringe the semen and stop nocturnal emission. It is mainly used to treat spermatorrhea, diarrhea at dawn caused by deficiency of the spleen and kidney and cold, and can also treat chronic cough, weak asthma, thirst due to loss of body fluids, spontaneous sweating, night sweats, and palpitations, insomnia and dreaminess caused by deficiency of heart and kidney yin and blood.

[0032] Raspberry: sweet in taste, slightly warm in nature, enters the liver and kidney meridians, and its main functions and effects: nourishes the liver and kidneys, astringes and consolidates the body, nourishes the kidneys and consolidates the essence, and also has the effects of promoting yang and improving eyesight. It is mainly used to treat kidney deficiency, spermatorrhea, enuresis, frequent urination and other symptoms. It can also treat kidney deficiency, impotence and blurred vision caused by liver and kidney deficiency.

[0033] Fried Plantago seed: light in taste, neutral in nature, enters the kidney, liver, and lung meridians. Efficacy and indications: diuretic and relieving stranguria without damaging kidney qi, able to diuresis, purge the virtual fire in the kidney, able to promote water and dampness, separate the clear from the turbid and stop diarrhea, also able to clear the liver and improve eyesight, clear the lungs and resolve phlegm, mainly treats urinary difficulty, edema, gonorrhea, summer-heat and dampness diarrhea, and can also treat red eyes, cataracts, blurred vision, and lung heat, cough, and excessive sputum.

[0034] This compound preparation can effectively relieve and treat the symptoms of sarcopenia by rationally combining these Chinese medicinal materials.

[0035] In certain specific embodiments, the above-mentioned Chinese medicine compound preparation for treating sarcopenia has its formula ingredients accurately measured according to the mass ratio, and specifically includes the following main ingredients: 30 to 50 parts of wolfberry, 30 to 50 parts of salt dodder seeds, 15 to 25 parts of raspberries, 8 to 12 parts of stir-fried plantain seeds, and 8 to 12 parts of schisandra chinensis. With this specific ratio, the compound preparation shows a more significant effect in treating sarcopenia.

[0036] In some other embodiments, the above-mentioned Chinese medicine compound preparation for treating sarcopenia, the formula components are accurately measured according to the mass ratio, and specifically include the following main ingredients: 40 parts of wolfberry, 40 parts of salt dodder seeds, 20 parts of raspberries, 10 parts of stir-fried plantain seeds, and 10 parts of schisandra chinensis. This specific ratio can not only effectively treat sarcopenia, but also ensure the safety and stability of the drug, providing patients with a more reliable treatment plan.

[0037] A method for preparing a Chinese medicine compound for treating sarcopenia, firstly, crushing the selected Chinese medicinal materials to ensure that the particles of the medicinal materials are small enough to facilitate the extraction of effective ingredients. Then, the crushed Chinese medicinal materials are put into a decoction container together, and distilled water equivalent to 6 to 10 times the weight of the Chinese medicinal materials is added for soaking. The purpose of soaking is to allow the effective ingredients in the Chinese medicinal materials to fully dissolve in water, thereby improving the effect of decoction.

[0038] After the soaking step is completed, the container is heated to gradually heat the water therein until it boils. After boiling, the heating state is maintained, and the Chinese medicinal materials are allowed to continue to be decocted at high temperature for a period of time so that the effective ingredients can be more fully released into the water. After the decoction is completed, the decoction liquid is separated from the medicinal residue by filtering, and the decoction liquid is collected.

[0039] Next, add 6 to 10 times the amount of distilled water to the previously obtained medicinal residue and continue to decoct. Similarly, heat the water to boiling, then keep boiling for a while, and separate the decoction and the medicinal residue by filtering again. The liquids obtained from these two decoctions are combined together and then concentrated on a hot water bath to remove excess water and concentrate the medicinal liquid to an appropriate concentration.

[0040] After the concentration is completed, the liquid medicine is placed at room temperature to cool naturally. After it cools to room temperature, it is packaged. The concentrated liquid medicine is packaged into appropriate containers, and finally the packaged liquid medicine is stored in a refrigerated environment to ensure the stability and effectiveness of the liquid medicine. In this way, a Chinese medicine compound for the treatment of sarcopenia is prepared and can be used at any time.

[0041] In some specific embodiments, the particle size of the pulverized material is set between 50 mesh and 100 mesh. Such a particle size range can ensure that the material has good reactivity and solubility in subsequent processing.

[0042] In some specific embodiments, when the material is soaked, the soaking time is set to be between 25 minutes and 35 minutes. Through such soaking time, the material can effectively absorb water fully, thereby preparing for subsequent processing steps.

[0043] In some embodiments, during the decoction process, the decoction time is controlled between 0.8 hours and 1.5 hours. Such a decoction time can ensure that the effective ingredients in the material are fully extracted, while avoiding excessive decoction that causes decomposition of the ingredients.

[0044] In some specific embodiments, during the concentration process, the goal of the concentration is to make 2.5 grams to 3.0 grams of medicinal material in every 1 ml of the liquid medicine. With such a concentration ratio, the concentration of the liquid medicine can be ensured to be moderate, which can ensure the efficacy and be convenient for patients to use.

[0045] A Chinese herbal compound can be used to prepare a drug for the treatment of sarcopenia. This compound can effectively relieve and improve the symptoms of sarcopenia through its unique formula and ingredients. In practical applications, this drug for the treatment of sarcopenia can adopt a variety of different drug dosage forms to meet the needs and preferences of different patients. Specifically, these dosage forms include traditional decoctions, which are convenient for patients to prepare and take at home; capsules, which are convenient to carry and take, and easy to swallow; tablets, which have a stable dose and a long shelf life; granules, which dissolve quickly and are easy to absorb; powders, which can be sprinkled directly on food for consumption; and pills, which are traditional forms and are easy to store for a long time. These different dosage forms provide patients with a variety of choices, making the treatment process more flexible and convenient.

[0046] Example 1 Preparation of a Chinese medicinal compound for treating sarcopenia

[0047] The Chinese medicine compound is prepared from the following raw materials by weight: 40g of wolfberry, 40g of salt dodder seed, 20g of raspberry, 10g of stir-fried plantain seed and 10g of schisandra chinensis are purchased from the Affiliated Hospital of Integrated Chinese and Western Medicine of Nanjing University of Chinese Medicine. The above Chinese medicines are first properly chopped into 50-100 meshes, placed in a decoction container, soaked in distilled water 8 times the weight of the Chinese medicine for 30 minutes, then heated, boiled for 1 hour after boiling, filtered, and the filtrate is collected; the residue is continued to be boiled with 8 times the distilled water, boiled for 1 hour, and filtered. The two decoctions are combined and concentrated in a hot water bath to a liquid equivalent to 2.88g of the medicinal material per 1ml. After cooling at room temperature, the decoctions are divided and stored in boxes at -20℃ for standby use.

[0048] Example 2 Establishment of Sarcopenia Mouse Model

[0049] Animal grouping and modeling

[0050] Experimental animals: 24 3-month-old SPF male C57BL / 6J mice were provided by the Experimental Animal Center of Jiangsu Institute of Traditional Chinese Medicine, with the experimental animal license number SYXK(Su)2021-0025. Approved by the Animal Experiment Ethics Committee (approval number: AEWC-20230610-323), all animals were adaptively fed in an isolated barrier environment (Animal Center of Jiangsu Provincial Hospital of Integrated Traditional Chinese and Western Medicine) for 2 weeks, with an initial average weight of (28.54±1.26) g, 4-5 mice per cage, temperature (22±2)℃, humidity (60±5)%, light 12h light and dark alternation, free access to water, and daily monitoring of their food intake and status.

[0051] After a 2-week adaptation period, the mice were randomly divided into two groups according to the different feed feeding: normal control group (Control, ordinary feed, feed ratio: 10% fat, 20% protein and 70% carbohydrate, n=6); high-fat diet group (HFD, high-fat feed, feed ratio: 60% fat, 20% protein and 20% carbohydrate, n=18).

[0052] The diagnosis of sarcopenia requires the combination of muscle strength, muscle quality, and muscle function. When muscle strength is reduced and muscle quantity or quality is low, sarcopenia can be diagnosed. The body composition of mice, i.e. muscle mass, is tested by testing grip strength and bone density (DXA) to determine whether the mouse model of sarcopenia is successful.

[0053] The mouse grip strength tester (Large and Small Mouse Grip Strength Tester TOW-13A, Tazheng Technology (China), grip plate area 230mm×250mm) was used to evaluate mouse muscle strength. The mice were adapted to the instrument for 3 days before the test. During the test, the mouse's tail was lifted and induced to grasp a rigid grid attached to a digital dynamometer. The tail of each mouse was gently pulled back, and the tension reading of the digital dynamometer was defined as the grip force before the mouse released the net. Five consecutive tests were performed on each mouse, and the average maximum limb muscle strength value (g) was calculated. The results are shown in Tables 1 and Figure 1 shown.

[0054] Table 1 Effects of high-fat diet on muscle strength in mice

[0055]

[0056] Compared with the control group, *P<0.05, **P<0.01.

[0057] Dual-energy X-ray bone densitometer DXA (bone density imager Ultra Focus DXA (Faxitron (USA)) was used to detect changes in body fat composition of mice. The weight of mice was measured, and 4% chloral hydrate solution was prepared at a dose of 1 ml / 100 g to anesthetize the mice to prevent them from moving during the test. After the mice were sedated and anesthetized, their limbs were fully spread out and they were placed prone on the DXA scanning detection platform. After the nose of the mice was aligned with the intersection of the red laser cross, the scan was started from head to tail. After the mouse scan was completed, the analysis software obtained the relevant data of the mouse body composition, including lean body weight, fat content, skeletal muscle mass, etc. The results are shown in Table 2.

[0058] Table 2 Effect of high-fat diet on muscle content in mice

[0059]

[0060] Compared with the control group, *P<0.05, **P<0.01.

[0061] After the model was established, the mice were deeply anesthetized and killed, and the lower limbs and muscles of the mice were removed. The skeletal muscle tissue on one side was immediately placed in a tissue fixative for fixation overnight, embedded in paraffin, and made into 4 μm tissue sections. Wax blocks of 3 mice were randomly selected from each group for sectioning and HE staining. The morphological quantitative analysis of the HE-stained sections of the muscle cross-sections was performed using ImageJ software. The results are shown in Table 3.

[0062] Table 3 Effects of high-fat diet on muscle cross-sectional area in mice

[0063]

[0064] Compared with the control group, *P<0.05, **P<0.01.

[0065] As can be seen from Tables 1-3, compared with the normal control group, the grip strength, skeletal muscle mass index, and muscle fiber cross-sectional area of ​​the high-fat diet group were significantly reduced, indicating that the sarcopenia mouse model was successfully constructed.

[0066] The TCM pathological mechanism of sarcopenia is mainly spleen and stomach weakness and liver and kidney deficiency. The high-fat mouse sarcopenia model constructed in this example can well simulate sarcopenia from the TCM mechanism for the following reasons:

[0067] 1. A high-fat diet can damage the spleen and stomach, leading to spleen deficiency. Spleen deficiency is one of the common causes of sarcopenia in traditional Chinese medicine theory. The spleen governs the muscles. If the spleen is weak, it cannot nourish the muscles, leading to muscle atrophy.

[0068] 2. A high-fat diet can lead to kidney deficiency. Kidney deficiency can lead to a lack of essence and blood, affecting muscle growth and development. In traditional Chinese medicine theory, the kidney is the "foundation of innateness" and is responsible for bone and marrow production. Insufficient bone marrow can lead to muscle weakness.

[0069] 3. A high-fat diet can lead to hyperlipidemia. In traditional Chinese medicine, hyperlipidemia is considered to be a pathological product of "phlegm dampness" and "blood stasis". These pathological products can lead to poor circulation of qi and blood, and muscles cannot get normal nutrition, resulting in sarcopenia.

[0070] 4. A high-fat diet can cause liver depression and qi stagnation. Liver depression and qi stagnation can affect the spleen and stomach functions, and further affect the physiological functions of muscles.

[0071] The high-fat mouse sarcopenia model of this embodiment is different from the tail-suspended mouse sarcopenia model. The tail-suspended mouse sarcopenia model is related to the "loss of nourishment of tendons and bones" and "blood deficiency" in traditional Chinese medicine theory, reflecting muscle atrophy caused by the loss of sufficient qi and blood nourishment. Therefore, the tail-suspended mouse sarcopenia model cannot simulate sarcopenia well from the mechanism of traditional Chinese medicine. Therefore, the embodiment of the present application adopts a high-fat mouse sarcopenia model that can better simulate the aging process of humans.

[0072] Example 3 Pharmacodynamics Experiment

[0073] After the sarcopenia model was established, the next step of the Chinese medicine compound intervention experiment was carried out. The 18 C57BL / 6J mice in the high-fat diet group were randomly divided into 3 groups according to their body weight, including a high-fat model group (equal volume of distilled water) (HFD), a low-dose Chinese medicine compound group (14.4g / kg / d) (WZ-L), and a high-dose Chinese medicine compound group (28.8g / kg / d) (WZ-H). The Chinese medicine prepared in Example 1 was given by gavage. At the same time, a normal control group (CON) of 6 mice was set up, and the normal control group (CON) and the high-fat model group (HFD) received the same volume of normal saline for gavage. The intervention ended after 3 months of gavage. See Table 4 for details. The method of Example 2 was used to test muscle strength, muscle content, skeletal muscle mass index SMI, muscle fiber cross-sectional area, etc. The experimental results are shown in Tables 5-7. HE staining of muscle tissue was performed, and the results are as shown Figure 7 shown.

[0074] Table 4

[0075]

[0076] Table 5 Effects of Chinese herbal compound on muscle strength of mice

[0077]

[0078] Compared with the control group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01.

[0079] Table 6 Effects of Chinese herbal compound on muscle content in mice

[0080]

[0081] Compared with the control group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01.

[0082] Table 7 Effects of Chinese herbal compound on muscle cross-sectional area of ​​mice

[0083]

[0084] Compared with the control group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01.

[0085] Table 5 shows the effect of the Chinese herbal compound on the muscle strength of mice. Statistical analysis showed that compared with the normal control group, the gripping strength of mice in the high-fat model group decreased significantly, which was statistically significant (**P<0.01). Compared with the high-fat model group, the gripping strength of mice in the low-dose group and high-dose group of the Chinese herbal compound was significantly improved, which was statistically significant (#P<0.05). The gripping strength of mice in the low-dose group and the high-dose group was significantly higher than that in the high-fat model group, but slightly lower than that in the normal control group. This shows that the Chinese herbal compound can effectively improve the negative effect of a high-fat diet on the muscle strength of mice, and both low and high doses can produce significant effects, but the effect of the high-dose group is slightly better than that of the low-dose group. The experimental results show that the Chinese herbal compound shows a potential positive effect in improving the muscle strength of mice, especially under high-fat diet conditions. This finding provides an experimental basis for the application of the Chinese herbal compound in resisting the decline of muscle strength.

[0086] In Table 6, we explored in detail the effect of the Chinese herbal compound on the muscle content of mice. Specifically, we conducted experiments on different groups of mice to observe the effect of the Chinese herbal compound on the skeletal muscle mass index (SMI). SMI refers to the ratio of the lean weight of the lower limbs of mice to their body weight, which can reflect the muscle content.

[0087] The experiment was divided into four groups, each with 3 mice. The first was the high-fat model group. The mice in this group established the model through a high-fat diet. The results showed that their skeletal muscle mass index was significantly lower than that of other groups, which was statistically significant (*P<0.05, **P<0.01). Next was the high-fat + low-dose Chinese medicine compound group. The mice in this group added a low-dose Chinese medicine compound on the basis of a high-fat diet. The results showed that the skeletal muscle mass index of mice in this group was significantly improved compared with the high-fat model group (#P<0.05). In addition, we also set up a high-fat + high-dose Chinese medicine compound group. The mice in this group added a high-dose Chinese medicine compound on the basis of a high-fat diet. The results showed that the skeletal muscle mass index of mice in this group was also significantly improved compared with the high-fat model group (#P<0.05), and was slightly improved compared with the low-dose group, but the difference was not statistically significant.

[0088] Finally, we also set up a normal control group, in which mice were fed a normal diet, and the results showed that their skeletal muscle mass index was 6.11±0.36. Compared with the high-fat model group, the skeletal muscle mass index of mice in the normal control group was significantly higher (##P<0.01), and compared with the Chinese medicine compound treatment group, the skeletal muscle mass index of mice in the normal control group also showed a certain advantage, but the difference was not statistically significant.

[0089] Through this experiment, we can see that the Chinese herbal compound can improve the problem of decreased muscle content in mice caused by a high-fat diet to a certain extent. Both low-dose and high-dose Chinese herbal compound can significantly improve the skeletal muscle mass index of mice, showing the potential application value of Chinese herbal compound in muscle protection.

[0090] Table 7 shows the effect of the Chinese herbal compound on the muscle cross-sectional area of ​​mice. From the data in Table 7, it can be seen that the muscle cross-sectional area of ​​mice in the high-fat model group was significantly lower than that in the normal control group, indicating that the high-fat diet had a negative impact on the muscle growth of mice. However, when the mice in the high-fat model group were treated with the Chinese herbal compound, their muscle cross-sectional area increased significantly in both the low-dose and high-dose groups. In particular, the muscle cross-sectional area of ​​the high-dose group was close to the level of the normal control group, showing the significant effect of the Chinese herbal compound in improving muscle cross-sectional area. These results indicate that the Chinese herbal compound has the potential to promote muscle growth and repair, especially in the sarcopenia model induced by a high-fat diet.

[0091] Figure 7 The images of muscle tissues of mice in different groups after HE staining are shown. By carefully observing these images, we can clearly see that both low-dose and high-dose interventions of the Chinese herbal compound have a positive effect on the muscle morphology of mice. Specifically, after treatment with the Chinese herbal compound, the muscle cells of the mice showed a more regular morphology, and the cell structure became more clearly visible. In addition, the connections between muscle fibers appeared orderly and compact, showing that the overall structure of the muscle tissue was significantly improved. These observations indicate that the Chinese herbal compound can effectively promote the health and functional recovery of muscle tissue at different dose levels.

[0092] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Chinese medicinal compound for treating sarcopenia, characterized in that: The invention comprises the following components by weight: 20-60 parts of wolfberry, 20-60 parts of salt dodder seeds, 10-30 parts of raspberries, 5-15 parts of stir-fried plantain seeds and 5-15 parts of schisandra chinensis.

2. The Chinese medicinal compound for treating sarcopenia according to claim 1, characterized in that: The invention comprises the following components by weight: 30-50 parts of wolfberry, 30-50 parts of salt dodder seeds, 15-25 parts of raspberries, 8-12 parts of stir-fried plantain seeds and 8-12 parts of schisandra chinensis.

3. The Chinese medicinal compound for treating sarcopenia according to claim 2, characterized in that: The composition comprises the following components by weight: 40 parts of wolfberry fruit, 40 parts of salt dodder seed, 20 parts of raspberry, 10 parts of stir-fried plantain seed and 10 parts of schisandra chinensis.

4. A method for preparing a Chinese medicinal compound for treating sarcopenia according to any one of claims 1 to 3, characterized in that: Firstly, the Chinese medicine is crushed, placed in a decoction container, soaked in distilled water 6-10 times the weight of the Chinese medicine, then heated, boiled again after boiling, filtered, and the filtrate is collected; the residue is added with 6-10 times the distilled water to continue decocting, boiling, and filtering; the two decoctions are combined and concentrated in a hot water bath; cooled at room temperature, then packaged and refrigerated for later use.

5. The preparation method according to claim 4, characterized in that: The particle size of the crushed particles is 50-100 meshes.

6. The preparation method according to claim 4, characterized in that: The soaking time is 25-35 minutes.

7. The preparation method according to claim 4, characterized in that: The decoction time is 0.8-1.5h.

8. The preparation method according to claim 4, characterized in that: The concentration is to concentrate until each 1 ml of the medicinal solution contains 2.5-3.0 g of the medicinal material.

9. Use of the Chinese medicinal compound according to any one of claims 1 to 3 in the preparation of a medicament for treating sarcopenia.

10. The use according to claim 9, characterized in that: The drug for treating sarcopenia is in the form of a decoction, capsule, tablet, granule, powder or pill.

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