Tabletting candy containing casein peptide and collagen tripeptide as well as preparation method and application thereof
Casein peptide prepared by enzymatic and spray-drying technology and collagen tripeptide tableting candy, combined with β-cyclodextrin and xylitol to mask the bitterness, solve the problems of bitterness and fishy smell of existing products, and achieve improved sleep and The effect of skin condition, suitable for large-scale production.
Patent Information
- Application Number
- CN202411418667.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-12
- Publication Date
- 2024-12-24
AI Technical Summary
The existing casein peptide and collagen tripeptide products have poor taste due to their bitter and fishy taste, and the lack of products that combine sleep improvement and supplement collagen tripeptide supplementation, making it difficult to effectively improve sleep quality and skin status.
A tablet candy formula of casein peptide and collagen tripeptide is produced by enzymatic decomposition and spray drying technology, combining β-cyclodextrin, xylitol and fruit concentrate powder to mask the bitter taste, and a stability is prepared through specific process steps. Good tablet candy, including 5-15 parts of casein peptide, 5-20 parts of collagen tripeptide, 1.0-3.0 parts of β-cyclodextrin, 5-10 parts of γ-aminobutyric acid, 30-50 xylitol Components of 10-20 parts of fruit concentrate powder and 0.1-1.0 parts of magnesium stearate.
Effectively improve sleep quality, improve skin condition, improve product taste and functional effect, and at the same time, the process is simple and suitable for large-scale production.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of food, and in particular relates to a tablet candy containing casein peptide and collagen tripeptide, and a preparation method and application thereof. Background Art
[0002] Casein peptides are a class of peptides with certain activity obtained by enzymatic hydrolysis, separation and purification of casein. Casein in cow's milk contains casein peptides that can promote sleep, which is also called casein sleep peptides. Many studies have shown that casein peptides in milk have a significant effect on various aspects of people's sleep. Although casein sleep peptides obtained by hydrolysis with specific proteases have the effect of improving sleep, they have a distinct bitter taste and poor oral suitability.
[0003] Collagen tripeptide is the smallest structural unit of collagen and can be fully absorbed by the human body. The hydrophilic natural moisturizing factor in collagen tripeptide has a stable triple helix structure, which can strongly lock in moisture and keep the skin moisturized and tender at all times. Collagen tripeptide has stronger permeability to the skin than collagen. It can penetrate the stratum corneum and bind to the skin epithelial cells, participate in and improve the metabolism of skin cells, enhance the activity of collagen in the skin, maintain the integrity of the stratum corneum moisture and fiber structure, improve the living environment of skin cells and promote the metabolism of skin tissue, enhance blood circulation, and achieve the purpose of moisturizing the skin. However, collagen tripeptide is mainly obtained by enzymatic hydrolysis, separation and purification of fish skin or fish scales, and has obvious fishy smell and bitterness. Collagen tripeptide is often used in oral beauty products, especially oral drinks are the most popular. At present, in the market, in order to ensure the taste requirements, the addition amount of collagen tripeptide in most collagen tripeptide foods is greatly limited, so the functional effect of consumption is also general.
[0004] Studies have shown that lack of sleep or poor sleep quality can affect endocrine disorders in the human body, which in turn can cause skin problems. Skin problems are largely caused by low levels of collagen tripeptide in the body, and part of the reason is endocrine disorders or sleep problems. There are currently few products on the market that combine improving sleep with supplementing collagen tripeptide to synergistically enhance the effects and solve skin problems. Summary of the invention
[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a tablet candy containing casein peptides and collagen tripeptides and a preparation method thereof. The prepared tablet candy containing casein peptides and collagen tripeptides has less bitterness, no obvious fishy smell, good stability, and can effectively improve sleep quality and improve skin condition.
[0006] To achieve the above object, the technical solution adopted by the present invention is: The compressed candy containing casein peptide and collagen tripeptide comprises the following components in parts by weight: 5-15 parts of casein peptide, 5-20 parts of collagen tripeptide, 1.0-3.0 parts of beta-cyclodextrin, 5-10 parts of gamma-aminobutyric acid, 30-50 parts of xylitol, 10-20 parts of fruit concentrate powder, and 0.1-1.0 parts of magnesium stearate.
[0007] Preferably, the compressed candy containing casein peptides and collagen tripeptides comprises the following components in parts by weight: 10 parts of casein peptide, 20 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 40 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0008] Preferably, the method for producing casein peptide comprises the following steps: Add water to casein at a mass ratio of 1:6-1:12 and stir, raise the temperature to 37-50°C, add protease with a casein mass of 0.05%-3.0% and digestive enzyme with a casein mass of 0.5%-4.0% in stages, perform two-step enzymolysis for a total of 3-8 hours, raise the temperature to inactivate the enzyme, and end the enzymolysis. The enzymolysis solution is filtered, concentrated and spray-dried to obtain casein peptides.
[0009] Furthermore, the source of the casein is cow's milk.
[0010] Furthermore, the protease includes one or more of papain, alkaline protease or chymotrypsin.
[0011] Furthermore, the digestive enzyme includes one or more of pepsin and pancreatin.
[0012] Preferably, the protease comprises papain or chymotrypsin, and the digestive enzyme is pepsin combined with pancreatic enzyme.
[0013] Furthermore, the mass of the protease added in the step is 0.1%-2.0% of the mass of the casein added.
[0014] Furthermore, the mass of the digestive enzyme added in the step is 0.5%-3.0% of the mass of the casein added.
[0015] Furthermore, the two-step enzymatic hydrolysis step specifically refers to: the first step of protease hydrolysis for 1-4 hours, and the second step of digestive enzyme hydrolysis for 2-4 hours.
[0016] Furthermore, the conditions for heating to inactivate the enzyme in the step are: heating to 100° C. and boiling for 15 minutes.
[0017] Preferably, the method for producing the collagen tripeptide comprises the following steps: (1) mixing tilapia fish skin with water, heating the mixture to 100° C.-110° C. and maintaining the mixture for 30 min-40 min, adding endoprotease to hydrolyze the fish skin collagen until the degree of hydrolysis is 7.0%-9.5%, heating the mixture to 80° C.-90° C. and maintaining the mixture for 20 min-40 min to inactivate the enzyme, and obtaining a tilapia fish skin collagen endoprotease hydrolyzate; (2) cooling the tilapia skin collagen endoprotease hydrolyzate to 40-60° C., adding 1%-5% (W / W) of the tilapia skin collagen endoprotease hydrolyzate of Bacillus amyloliquefaciens protease, and performing enzymolysis for 9-15 hours; (3) after the tilapia skin collagen endoprotease hydrolyzate treated with Bacillus amyloliquefaciens protease is hydrolyzed to a degree of hydrolysis of 14.21%-18.80%, the temperature is raised to 80° C.-90° C. and maintained for 20 min-40 min to inactivate the enzyme; (4) removing the residue by centrifugation, concentrating, and freeze-drying to obtain tilapia skin collagen tripeptide.
[0018] Preferably, the γ-aminobutyric acid is produced by using sodium L-glutamate as raw material through the steps of Lactobacillus shilgardii fermentation, heat sterilization, cooling, activated carbon treatment, filtration, addition of blending auxiliary materials (starch), spray drying and the like.
[0019] Preferably, the fruit concentrated powder is one or more of blueberry concentrated powder, cranberry concentrated powder and passion fruit concentrated powder.
[0020] Furthermore, the fruit concentrated powder is obtained by spray-drying fruit concentrated juice by adding maltodextrin as a carrier.
[0021] Furthermore, the proportion of fruit concentrated juice in the fruit concentrated powder is greater than 65%.
[0022] The present invention also provides a method for preparing the compressed candy containing casein peptide and collagen tripeptide, comprising the following steps: (1) After mixing casein peptide and collagen tripeptide, add appropriate amount of water and stir to dissolve for 10-20 minutes. After uniform dissolution, add β-cyclodextrin and continue to stir and dissolve for 10-20 minutes to obtain a mixed solution of casein peptide, collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide.
[0023] Furthermore, in the step (1), an appropriate amount of water is added in an amount that is 9 times the total weight of the casein peptide and the collagen tripeptide.
[0024] The present invention also provides the use of the compressed candy containing casein peptide and collagen tripeptide in improving sleep and improving skin condition.
[0025] Compared with the prior art, the beneficial effects of the present invention are: (1) The bitterness of the casein peptide and collagen tripeptide added in the present invention is mainly caused by the exposure of some hydrophobic amino acid peptides contained therein. The casein peptide and collagen tripeptide are pre-mixed and dissolved, and then the hydrophobic amino acid peptides can be embedded in the hydrophobic cavity of β-cyclodextrin to cover up their bitterness. At the same time, xylitol and fruit concentrate powder are added to coordinate and blend with the above ingredients, further improving the taste of the compressed candy; (2) The casein peptide and collagen tripeptide of the present invention work together to most effectively improve sleep quality, delay skin aging, and enable people to have good skin condition with adequate sleep; (3) The preparation process of the present invention is simple, easy to operate, and suitable for scale-up production to achieve large-scale production. DETAILED DESCRIPTION
[0026] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Example 1
[0027] The compressed candy containing casein peptide and collagen tripeptide comprises the following components in parts by weight: 10 parts of casein peptide, 20 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 40 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0028] The preparation method comprises the following steps: (1) After mixing casein peptide and collagen tripeptide, add 9 times water and stir and dissolve for 10-20 minutes. After dissolving evenly, add β-cyclodextrin and continue stirring and dissolving for 10-20 minutes to obtain a mixed solution of casein peptide, collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide. Example 2
[0029] The compressed candy containing casein peptide and collagen tripeptide comprises the following components in parts by weight: 15 parts of casein peptide, 15 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 40 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0030] The preparation method comprises the following steps: (1) After mixing casein peptide and collagen tripeptide, add 9 times water and stir and dissolve for 10-20 minutes. After dissolving evenly, add β-cyclodextrin and continue stirring and dissolving for 10-20 minutes to obtain a mixed solution of casein peptide, collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide. Example 3
[0031] The compressed candy containing casein peptide and collagen tripeptide comprises the following components in parts by weight: 20 parts of casein peptide, 10 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 40 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0032] The preparation method comprises the following steps: (1) After mixing casein peptide and collagen tripeptide, add 9 times water and stir and dissolve for 10-20 minutes. After dissolving evenly, add β-cyclodextrin and continue stirring and dissolving for 10-20 minutes to obtain a mixed solution of casein peptide, collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide.
[0033] Comparative Example 1 The compressed candy containing collagen tripeptide comprises the following components in parts by weight: 20 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 50 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0034] The preparation method comprises the following steps: (1) Add 9 times the amount of water to the collagen tripeptide and stir and dissolve for 10-20 minutes. After the mixture is evenly dissolved, add β-cyclodextrin and continue stirring and dissolving for 10-20 minutes to obtain a mixture of collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added thereto for mixing and tableting, thereby obtaining a tablet candy containing collagen tripeptide.
[0035] Comparative Example 2 The compressed candy containing casein peptide and collagen tripeptide comprises the following components in parts by weight: 10 parts of casein peptide, 20 parts of collagen tripeptide, 10 parts of gamma-aminobutyric acid, 42.3 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
[0036] The preparation method comprises the following steps: (1) mixing casein peptide and collagen tripeptide evenly to obtain a mixed dry powder; (2) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (1), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (3) The soft material obtained in step (2) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide.
[0037] The present invention provides comparative example 1 (comparative example 1 does not contain casein peptide, and except for xylitol, the other components are exactly the same as those of embodiment 1) and comparative example 2 (comparative example 2 does not contain β-cyclodextrin, except for xylitol, the other components are exactly the same as those of embodiment 1, and the preparation method does not have a spray drying step).
[0038] Test example: Taste evaluation and sleep and beauty effect tests 100 women aged 25-45 with sub-health conditions such as sleep quality problems were selected as volunteers to conduct effect testing and taste evaluation of compressed candies, where the taste score was 1-10 points, 1 point for the worst taste, and 10 points for the best taste. The 100 volunteers were divided into 5 groups, and they tasted the compressed candies of Examples 1-3 and Comparative Examples 1-2, respectively. Each person consumed 2 tablets of compressed candies (specification 0.8g / tablet) 30 minutes before going to bed every day for 1 month, and wore a sleep bracelet to sleep. The sleep state was evaluated by recording the time taken to fall asleep and the sleep quality score, which was divided into no obvious improvement, improvement, and good improvement. The changes in skin state were evaluated by detecting skin moisture, which was divided into no obvious improvement, improvement, and good improvement. During the test, the eating and resting habits of all volunteers remained consistent. The test results are shown in Tables 1 and 2: Table 1 Taste evaluation
[0039] Table 2 Sleep and beauty effect test results
[0040] As can be seen from Table 1, the taste of Example 1 is significantly better than that of other examples and comparative examples. The present invention can effectively mask the bitterness of casein peptide and collagen tripeptide by dissolving the casein peptide and collagen tripeptide in water and then embedding them with β-cyclodextrin, and then granulating and tableting them after spray drying.
[0041] As can be seen from Table 2, the effects of improving sleep are similar among Example 1, Example 2, Example 3 and Comparative Example 2, and are all significantly better than Comparative Example 1. The effect of improving skin condition is Example 1>Comparative Example 2>Example 2>Example 3>Comparative Example 1.
[0042] It should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.
Claims
1. A tableted candy containing casein peptide and collagen tripeptide, characterized in that: Contains the following components in parts by weight: 5-15 parts of casein peptide, 5-20 parts of collagen tripeptide, 1.0-3.0 parts of beta-cyclodextrin, 5-10 parts of gamma-aminobutyric acid, 30-50 parts of xylitol, 10-20 parts of fruit concentrate powder, and 0.1-1.0 parts of magnesium stearate.
2. The tablet candy containing casein peptide and collagen tripeptide according to claim 1, characterized in that: Contains the following components in parts by weight: 10 parts of casein peptide, 20 parts of collagen tripeptide, 2.3 parts of beta-cyclodextrin, 10 parts of gamma-aminobutyric acid, 40 parts of xylitol, 17 parts of fruit concentrate powder, and 0.7 parts of magnesium stearate.
3. The tablet candy containing casein peptide and collagen tripeptide according to claim 2, characterized in that: The fruit concentrated powder is one or more of blueberry concentrated powder, cranberry concentrated powder and passion fruit concentrated powder.
4. The tablet candy containing casein peptide and collagen tripeptide according to claims 1-3, characterized in that: The fruit concentrated powder is obtained by spray-drying fruit concentrated juice by adding maltodextrin as a carrier, and the proportion of fruit concentrated juice in the fruit concentrated powder is greater than 65%.
5. The tablet candy containing casein peptide and collagen tripeptide and the preparation method thereof according to claims 1-4, characterized in that: The steps include: (1) After mixing casein peptide and collagen tripeptide, add appropriate amount of water and stir to dissolve for 10-20 minutes. After uniform dissolution, add β-cyclodextrin and continue to stir and dissolve for 10-20 minutes to obtain a mixed solution of casein peptide, collagen tripeptide and β-cyclodextrin; (2) spray drying the mixed solution in step (1) to obtain a mixed dry powder; (3) Gamma-aminobutyric acid and xylitol are crushed separately, passed through an 80-mesh sieve, and then mixed evenly with the mixed dry powder and fruit concentrate powder obtained in step (2), and wet granulated using a 70%-80% ethanol solution to prepare a soft material; (4) The soft material obtained in step (3) is dried by a boiling drying device, and magnesium stearate is added for mixing and tableting to obtain a tablet candy containing casein peptides and collagen tripeptide.
6. The tablet candy containing casein peptide and collagen tripeptide and the preparation method thereof according to claims 1-5, characterized in that: In the step (1), an appropriate amount of water is added in an amount of 9 times the total weight of the casein peptide and the collagen tripeptide.
7. The tablet candy containing casein peptide and collagen tripeptide according to claim 6, characterized in that: Application in improving sleep and improving skin condition.