Culture medium for soilless potted Chinese roses and preparation method of culture medium

Abstract

The invention belongs to the technical field of gardening flower cultivation, and particularly relates to a soilless potted Chinese rose cultivation medium and a preparation method thereof.The medium is prepared from humus soil, perlite, vermiculite, coco coir, peat and other components according to a specific proportion, and good air permeability, water retention and nutrient balance are guaranteed. In addition, 5% of corn straw biochar and 1 * 10 < 8 > CFU / mL of bacillus subtilis and bacillus megaterium bacterial liquid are added into the substrate, so that the microbial activity and the root health are enhanced. Experimental data results show that the culture medium remarkably improves the growth speed, the flowering phase durability and the ornamental value of the Chinese roses, is suitable for potted plant culture in urban families and gardening landscapes, and has wide application prospects and economic benefits.

Description

Technical Field

[0001] The invention relates to the technical field of horticultural flower cultivation, in particular to a cultivation medium for soilless potted roses and a preparation method thereof. Background Art

[0002] Rose (Rosa spp.) is widely used in garden landscapes and home potted plants because of its diverse flower colors and long flowering period. However, traditional soil cultivation methods often face problems such as soil compaction, nutrient imbalance and poor aeration, which in turn affect the growth and ornamental effect of roses. In order to overcome these limitations, soilless cultivation technology has been widely used in recent years, among which the selection and improvement of cultivation substrates are important factors that determine the growth effect of plants.

[0003] Soil matrix improvement is important for plant growth. The main ingredients include humus, peat, coconut bran, vermiculite, perlite, etc. Compared with garden soil cultivation, it optimizes the soil environment and provides more suitable conditions for plant growth, which plays a vital role in the healthy growth of plants and high-yield and high-quality production. The specific effects include the following:

[0004] 1) Improve soil structure. Soil matrix improvement can improve the physical structure of the soil, increase soil permeability, water retention and fertilizer retention, and facilitate the growth and development of plant roots. This is crucial for improving soil permeability, drainage, and reducing salt accumulation in the soil.

[0005] 2) Provide rich nutrients. Soil matrix improvement can add organic matter, minerals and other nutrients to provide rich nutrients required by plants. This is crucial for the growth, flowering and fruiting stages of plants, especially when the original nutrients in the soil are insufficient or the inorganic content is low.

[0006] 3) Adjusting pH: Soil matrix improvement can adjust the pH of the soil to make it more suitable for plant growth. Some plants have clear requirements for soil pH. Through matrix improvement, the pH value of the soil can be adjusted to create a more suitable growth environment.

[0007] 4) Improve soil water retention capacity. Soil matrix improvement can improve soil water retention capacity and reduce water loss. This can better meet the water needs of plants in arid areas or when soil water management is difficult.

[0008] 5) Reduce the pressure of soil pests and diseases. Reasonable soil matrix improvement can reduce the occurrence of pests and diseases in the soil and improve the plant's ability to resist pests and diseases.

[0009] In addition to the physical properties of the matrix material, studies have shown that plant growth promoting rhizobacteria (PGPR) have a significant promoting effect on plant growth. PGPR can improve the growth performance of plants by improving the plant root environment, enhancing disease resistance and promoting nutrient absorption. In particular, in a soilless culture system, PGPR can make up for the lack of natural soil microorganisms in the matrix, and further improve the root health and growth performance of roses. However, the existing soilless culture matrix has not yet fully utilized the advantages of PGPR technology, and it is urgent to develop a soilless culture matrix combined with PGPR to optimize the growth environment and stress resistance of rose potted plants. Based on this, the present invention aims to develop a soilless culture matrix containing PGPR, providing better air permeability, water retention and nutrient balance, and enhancing the growth, disease resistance and stress resistance of roses through the action of PGPR, which is particularly suitable for potted cultivation in urban families and gardening landscapes. Summary of the invention

[0010] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0011] Therefore, the purpose of the present invention is to provide a cultivation substrate for soilless potted roses and a preparation method thereof, which can optimize the physical and chemical properties of the substrate, improve the growth performance, stress resistance and nutrient absorption efficiency of roses, solve the soil problems existing in traditional cultivation, and promote the healthy growth and efficient cultivation of roses.

[0012] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions:

[0013] A soilless potted rose cultivation medium, comprising: the medium is prepared by the following components in volume ratio:

[0014] Humus soil: 30% to 50%;

[0015] Perlite: 15% to 25%;

[0016] Vermiculite: 10% to 15%;

[0017] Coconut peat: 10% to 20%;

[0018] Peat: 10% to 20%.

[0019] As a preferred solution of the cultivation matrix of the soilless potted rose described in the present invention, corn straw biochar is also added to the matrix, and the addition ratio of the corn straw biochar is 5% of the volume of the matrix in claim 1.

[0020] As a preferred embodiment of the cultivation medium for the soilless potted rose described in the present invention, Bacillus subtilis and Bacillus megaterium bacterial liquid are also added to the medium, the concentration of the bacterial liquid is 1×10^8 CFU / mL, and the addition amount is 20 mL / kg.

[0021] A method for preparing a soilless potted rose cultivation medium comprises: step 1, preparing humus, perlite, vermiculite, coconut bran and peat, wherein the humus, perlite, vermiculite, coconut bran and peat are free of pests and diseases and pollution;

[0022] Step 2: Screen the humus soil to remove large impurities;

[0023] Step 3: In a clean container, add the humus, perlite, vermiculite, coconut bran and peat screened in step 2 in order according to the volume ratio in claim 1;

[0024] Step 4: Use a shovel or a stirring rod to fully stir the materials in step 3 and mix them evenly to obtain a mixed matrix;

[0025] Step 5: adding corn straw biochar to the mixed matrix at a ratio of 5% of the volume of the mixed matrix in step 4, and stirring to evenly distribute the rice straw biochar in the mixed matrix;

[0026] Step 6, inoculate Bacillus subtilis and Bacillus megaterium strains at a 1% (v / v) inoculum into 100 mL LB liquid culture medium sterilized at 121° C. for 30 minutes in advance, activate and culture at 30° C. and 160 r / min, then centrifuge at 5,000 r / min for 5 minutes, collect the bacteria and dilute with sterile water, and use a UV spectrophotometer to adjust the OD600 of the bacterial solution to 0.5 to obtain Bacillus subtilis and Bacillus megaterium bacterial solutions;

[0027] Step 7, adding the Bacillus subtilis and Bacillus megaterium bacterial solutions in step 6 to the mixed matrix in step 5 at a ratio of 20 mL / kg;

[0028] Step 8: Use a shovel to stir the mixed matrix in step 7 to evenly infiltrate the bacterial solution into the mixed matrix;

[0029] Step nine: moisten the mixed matrix in step eight to a suitable water content, wherein the suitable water content is 60%-70% of the weight of the matrix.

[0030] As a preferred embodiment of the method for preparing a soilless potted rose cultivation medium according to the present invention, the mixed matrix components obtained in step nine are evenly distributed.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] 1. The present invention not only provides excellent air permeability and water retention through scientific ingredient ratio, but also ensures balanced supply of nutrients and creates an ideal growth environment; humus provides rich organic matter for the matrix, perlite enhances drainage performance, vermiculite improves water retention capacity, and coconut bran and peat improve the characteristics of water retention and nutrient release.

[0033] 2. The corn straw biochar added in the present invention helps to increase the microbial activity of the matrix, promote root health, and further improve the plant's absorption efficiency of water and nutrients.

[0034] 3. The present invention adds root growth-promoting strains Bacillus subtilis and Bacillus megaterium, so that the matrix not only enhances the disease resistance of the plant, but also improves its stress resistance, so that the rose performs better when facing environmental pressure. DETAILED DESCRIPTION

[0035] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below.

[0036] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0037] Secondly, the present invention is described in detail with reference to schematic diagrams. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0038] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be described in further detail below.

[0039] The present invention provides a cultivation medium for soilless potted roses and a preparation method thereof, wherein the medium is prepared by the following components in volume ratio:

[0040] Humus soil: 30% to 50%;

[0041] Perlite: 15% to 25%;

[0042] Vermiculite: 10% to 15%;

[0043] Coconut peat: 10% to 20%;

[0044] Peat: 10% to 20%.

[0045] Example 1

[0046] A specific preferred matrix formula is: 40% humus, 20% perlite, 10% vermiculite, 10% coconut bran, and 10% peat.

[0047] The specific preparation method is as follows:

[0048] Step 1: prepare humus, perlite, vermiculite, coconut husk and peat, wherein the humus, perlite, vermiculite, coconut husk and peat are free of pests and diseases and pollution;

[0049] Step 2: Screen the humus soil to remove large impurities;

[0050] Step 3: In a clean container, add the humus, perlite, vermiculite, coconut bran and peat screened in step 2 in order according to the volume ratio in claim 1;

[0051] Step 4: Use a shovel or a stirring rod to fully stir the materials in step 3 and mix them evenly to obtain a mixed matrix;

[0052] Step 5: adding corn straw biochar to the mixed matrix at a ratio of 5% of the volume of the mixed matrix in step 4, and stirring to evenly distribute the rice straw biochar in the mixed matrix;

[0053] Step 6, inoculate Bacillus subtilis and Bacillus megaterium strains at a 1% (v / v) inoculum into 100 mL LB liquid culture medium sterilized at 121° C. for 30 minutes in advance, activate and culture at 30° C. and 160 r / min, then centrifuge at 5,000 r / min for 5 minutes, collect the bacteria and dilute with sterile water, and use a UV spectrophotometer to adjust the OD600 of the bacterial solution to 0.5 to obtain Bacillus subtilis and Bacillus megaterium bacterial solutions;

[0054] In this embodiment, the preferred LB liquid culture medium formula is: tryptone (10 g / L), yeast extract (5 g / L), sodium chloride (10 g / L), pH 7.4.

[0055] Step 7, adding the Bacillus subtilis and Bacillus megaterium bacterial solutions in step 6 to the mixed matrix in step 5 at a ratio of 20 mL / kg;

[0056] Step 8: Use a shovel to stir the mixed matrix in step 7 to evenly infiltrate the bacterial solution into the mixed matrix;

[0057] Step nine: moisten the mixed matrix in step eight to a suitable water content, wherein the suitable water content is 60%-70% of the weight of the matrix.

[0058] Example 2

[0059] A cultivation medium for soilless potted roses, the preparation method of which is consistent with that of Example 1, and the volume ratio of the medium components is: 30% humus, 15% perlite, 10% vermiculite, 20% coconut bran, and 20% peat.

[0060] Example 3

[0061] A cultivation medium for soilless potted roses, the preparation method of which is consistent with that of Example 1, and the volume ratio of the medium components is: 35% humus, 25% perlite, 15% vermiculite, 10% coconut bran, and 10% peat.

[0062] Example 4

[0063] A cultivation medium for soilless potted roses, the preparation method of which is consistent with that of Example 1, and the volume ratio of the medium components is: 50% humus, 15% perlite, 10% vermiculite, 10% coconut bran, and 10% peat.

[0064] Example 5

[0065] A cultivation medium for soilless potted roses, the preparation method of which is consistent with that of Example 1, and the volume ratio of the medium components is: 30% humus, 20% perlite, 15% vermiculite, 15% coconut bran, and 15% peat.

[0066] A comparative cultivation matrix was set up, which was different from Example 1 in that no biochar and bacterial solution were added to the matrix.

[0067] Comparative Example 1

[0068] A cultivation medium for soilless potted roses, wherein the volume ratio of the medium components is: 40% humus, 20% perlite, 10% vermiculite, 10% coconut bran and 10% peat.

[0069] Comparative Example 2

[0070] A cultivation medium for soilless potted roses, wherein the volume ratio of the medium components is: 30% humus, 15% perlite, 10% vermiculite, 20% coconut bran and 20% peat.

[0071] Comparative Example 3

[0072] A cultivation medium for soilless potted roses, wherein the volume ratio of the medium components is: 35% humus, 25% perlite, 15% vermiculite, 10% coconut bran and 10% peat.

[0073] Comparative Example 4

[0074] A cultivation medium for soilless potted roses, wherein the volume ratio of the medium components is: 50% humus, 15% perlite, 10% vermiculite, 10% coconut bran and 10% peat.

[0075] Comparative Example 5

[0076] A cultivation medium for soilless potted roses, wherein the volume ratio of the medium components is: 30% humus, 20% perlite, 15% vermiculite, 15% coconut bran and 15% peat.

[0077] The present invention adopts flower pots with specifications of upper diameter of 15.7 cm, bottom diameter of 12.4 cm, and height of 16.5 cm, and soil capacity of 2 L for rose cultivation, three rose seedlings with basically the same growth potential are planted in each pot, and 8 pots are provided for each formula. During the entire growth cycle of the rose, nutrient solution is irrigated once every 20 days to ensure comprehensive nutrition, and bees are released during the flowering period to improve the pollination rate.

[0078] Determination of morphological indicators of the present invention. In order to ensure the accuracy of the test, 5 rose seedlings with good growth and uniformity were randomly selected from each pot. First, the root soil was thoroughly rinsed with deionized water and dried. Next, the plant height and root length of the rose were measured using a ruler with an accuracy of 0.1 cm. Subsequently, the 5 selected rose seedlings were separated to obtain the root and stem parts, respectively. After measuring the fresh weight of these three parts, they were placed in an oven at 105 degrees Celsius for fixing for 20 minutes. Then, the sample was transferred to an oven at 60 degrees Celsius until its weight remained constant. Finally, an analytical balance with an accuracy of 0.0001 grams was used to measure the dry weight of the root and stem parts, respectively.

[0079] When collecting and processing root soil samples, first shake off the soil loosely bound to the roots. Then, use a small brush to gently brush off the soil tightly bound to the roots and pass it through a 2mm sieve.

[0080] Chlorophyll determination method: uniformly cut about 0.1g of fresh leaves from the third cotyledon of rose, cut into pieces, add 5ml of 95% ethanol, shake and extract for 48h in the dark, use 95% ethanol as the blank control group, and calculate the chlorophyll a, chlorophyll b, carotenoids and total chlorophyll contents according to the following equation.

[0081] Chlorophyll a (mg / gFW) = (13.95 × D665-6.88 × D649) × 0.005 ÷ m

[0082] Chlorophyll b (mg / gFW) = (24.96 × D649-7.32 × D665) × 0.005 ÷ m

[0083] Carotenoids (mg / gFW) = (1000×A470-1.29×Chla-53.78×Chlb) ÷ 220

[0084] Total chlorophyll (mg / gFW) = Chla + Chlb = (18.08 × D649 + 6.63 × D665) × 0.005 ÷ m

[0085] Wherein, m is the mass of the weighed rose leaves, D470 is the absorbance of the sample extract at 470nm, D649 is the absorbance of the sample extract at 649nm, and D665 is the absorbance of the sample extract at 665nm.

[0086] Determination of soil physicochemical properties of the present invention: pH is extracted with a water-soil ratio of 2.5:1 and measured by a pH meter. Conductivity is extracted with a water-soil ratio of 5:1 and measured by a conductivity meter. The cation exchange capacity is determined by sodium acetate-flame photometry. Steps: After weighing the soil in a centrifuge tube, add sodium acetate, shake for 5 minutes and then centrifuge, and repeat the extraction with sodium acetate 4 times. Then add ammonium acetate, shake and centrifuge, pour the supernatant into a volumetric flask, repeat twice, and finally make up the volume with ammonium acetate and measure with a flame photometer. The soil organic matter content is determined by potassium dichromate volumetric method-external heating method. After weighing the soil in a test tube, add concentrated sulfuric acid, add potassium dichromate, heat in an oil bath for 5 minutes, move into a triangular flask after cooling, add o-phenanthroline indicator, and titrate with ferrous sulfate. The nitrate nitrogen content is determined by ultraviolet spectrophotometry correction factor method, and ammonium nitrogen is determined by KCl extraction-indophenol blue colorimetry. The available phosphorus content is extracted with sodium bicarbonate, molybdenum antimony reagent is used for colorimetry, and the spectrophotometer is used for determination. Steps: After weighing the soil in a plastic bottle, add sodium bicarbonate and activated carbon, shake for 30 minutes, take out and filter, absorb 10ml, add molybdenum antimony reagent, make up to 50ml, and use a spectrophotometer for colorimetry at 880nm. The available potassium content is determined by ammonium acetate extraction and flame photometry. Steps: After weighing the soil in a plastic bottle, add ammonium acetate, shake for 30 minutes, take out and filter, and determine with a flame photometer.

[0087] According to the data analysis of Table 1, the growth performance of Chinese rose shows obvious differences. In Example 1, the plant height is 96.59cm and the root length is 19.33cm, while the plant height of Example 2 is increased to 103.22cm, and the root length reaches 26.33cm, showing the best growth state. The growth data of Example 3 and Example 4 are respectively 92.67cm and 90.33cm plant height, and the root length is also between 19.65cm and 21.67cm, indicating the influence of different formulas on the growth effect. In terms of stem thickness and stem fresh weight, the stem thickness of Example 2 is 6.7mm, and the stem fresh weight is 19.65g, which is the highest among all embodiments, showing good growth intensity. The stem fresh weight of Example 5 also reached 21.19g, indicating that the matrix can effectively promote the development of stem. In terms of root growth, the root fresh weight of Example 5 is 24.61g, and the root dry weight is 7.73g, which is better than the various indicators of the comparison group, showing stronger root growth ability. The data in the comparative group are generally lower, such as the plant height of comparative example 1 is only 70.33 cm, and the root length is 16.67 cm, which are lower than all the examples. The cultivation substrates used in Examples 1 to 5 show significant advantages in the growth performance of roses, which indicates that the substrate can significantly enhance root growth, thereby improving the water absorption and nutrient acquisition ability of plants.

[0088] Table 1 Effects of different treatments on rose growth indicators

[0089]

[0090] According to the data analysis in Table 2, the photosynthetic capacity of plants with different treatments can be analyzed. The chlorophyll a content of Example 2 is 23.59 mg / L, which is significantly higher than other groups, indicating that the treatment conditions can effectively promote the synthesis of photosynthetic pigments, thereby enhancing the photosynthetic efficiency of plants. The chlorophyll b content is 13.87 mg / L, which is also at a high level, which further supports the ability of plants to capture light energy during photosynthesis. In addition, the total chlorophyll content of Example 2 is 37.46 mg / L, showing its advantages in enhancing plant photosynthetic capacity and promoting growth. In contrast, the chlorophyll b content of Example 3 is abnormally high, reaching 41.95 mg / L, while the contents of chlorophyll a and carotenoids are relatively low, which may indicate that the treatment has a special effect on plants, resulting in changes in the photosynthetic mechanism. The total chlorophyll reaches 52.5 mg / L, and despite its low chlorophyll a content, it still shows good performance in overall photosynthetic capacity. In other control groups, the contents of chlorophyll a and b were generally lower than those in Example 2, especially in Comparative Example 2, where the total chlorophyll was only 14.75 mg / L, showing a relatively weak photosynthetic capacity. This indicates that under the condition of optimizing the cultivation substrate, the photosynthesis and growth potential of the plant have been effectively improved.

[0091] Table 2 Effects of different treatments on chlorophyll

[0092]

[0093] According to the data analysis of Table 3, the pH values ​​of the embodiments are all between 6.90 and 7.20, indicating that the soil is neutral and is conducive to the nutrient absorption of plants. The electrical conductivity (EC) ranges from 177.12 to 220.33 μS / cm, indicating that the soil is rich in dissolved nutrients and can support the healthy growth of plants. The organic matter content of the embodiment is between 9.10% and 9.90%, which is higher than that of the control group. The increase in organic matter not only improves the water holding capacity and aeration of the soil, but also promotes microbial activity, which helps the decomposition and release of nutrients. The nitrate nitrogen content in the embodiment is as high as 25.03 mg / kg, which is crucial to promoting plant growth because nitrate nitrogen is the main form of plant nutrient absorption. The ammonium nitrogen content also performs well, with a maximum of 71.67 mg / kg, which further improves the fertility of the soil. In addition, the content of available phosphorus is 55.58 mg / kg to 102.73 mg / kg in the embodiment, which can effectively support the development of plant roots and promote flowering and fruiting. The effective potassium content is between 278.40 and 321.08 mg / kg, and Example 5 in particular performs excellently, which helps to improve the stress resistance and photosynthesis efficiency of plants.

[0094] Table 3 Effects of different treatments on soil physical and chemical properties

[0095]

[0096] The matrix soil used in the embodiment shows significant advantages in promoting rose growth and photosynthesis. Specifically, the plant height and root length of Example 2 reach 103.22cm and 26.33cm respectively, which are significantly higher than the control group, indicating that the matrix can effectively enhance plant growth. In addition, the chlorophyll a content (23.59mg / L) and total chlorophyll (37.46mg / L) of Example 2 are the highest, showing its excellent photosynthesis ability. The pH value of the soil is maintained between 6.90 and 7.20, which is conducive to the nutrient absorption of plants.

[0097] In summary, the study found that the cultivation medium of Example 2 is more suitable for rose cultivation and has good potential in improving soil physical and chemical properties, promoting root development and enhancing plant stress resistance.

[0098] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A cultivation medium for soilless potted roses, characterized in that: include: The matrix is ​​prepared by the following components in volume ratio: Humus soil: 30% to 50%; Perlite: 15% to 25%; Vermiculite: 10% to 15%; Coconut bran: 10% to 20%; Peat: 10% to 20%.

2. The cultivation medium for a soilless potted rose according to claim 1, characterized in that: Corn straw biochar is also added to the matrix, and the addition ratio of the corn straw biochar is 5% of the volume of the matrix in claim 1.

3. The cultivation medium for a soilless potted rose according to claim 1, characterized in that: Bacillus subtilis and Bacillus megaterium bacterial liquids are also added to the matrix, the concentration of the bacterial liquids is 1×10^8 CFU / mL, and the added amount is 20 mL / kg.

4. A method for preparing a soilless potted rose cultivation medium, characterized in that: The method comprises: Step 1: prepare humus, perlite, vermiculite, coconut husk and peat, wherein the humus, perlite, vermiculite, coconut husk and peat are free of pests and diseases and pollution; Step 2: Screen the humus soil to remove large impurities; Step 3: In a clean container, add the humus, perlite, vermiculite, coconut bran and peat screened in step 2 in order according to the volume ratio in claim 1; Step 4: Use a shovel or a stirring rod to fully stir the materials in step 3 and mix them evenly to obtain a mixed matrix; Step 5: adding corn straw biochar to the mixed matrix at a ratio of 5% of the volume of the mixed matrix in step 4, and stirring to evenly distribute the rice straw biochar in the mixed matrix; Step 6: Inoculate Bacillus subtilis and Bacillus megaterium strains at a 1% (v / v) inoculum into 100 mL LB liquid culture medium sterilized at 121°C for 30 minutes in advance, activate the culture at 30°C and 160 r / min, and then centrifuge at 5,000 r / min for 5 minutes. Collect the bacteria and dilute with sterile water. Use a UV spectrophotometer to adjust the OD600 of the bacterial solution to 0.5 to obtain Bacillus subtilis and Bacillus megaterium bacterial solutions; Step 7, adding the Bacillus subtilis and Bacillus megaterium bacterial solutions in step 6 to the mixed matrix in step 5 at a ratio of 20 mL / kg; Step 8: Use a shovel to stir the mixed matrix in step 7 to evenly infiltrate the bacterial solution into the mixed matrix; Step nine: moisten the mixed matrix in step eight to a suitable water content, wherein the suitable water content is 60%-70% of the weight of the matrix.

5. The method for preparing a soilless potted rose cultivation medium according to claim 4, characterized in that: The mixed matrix components obtained in step nine are evenly distributed.

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