Medical application of hesperidin in prevention of obesity caused by high fat diet

Abstract

The invention relates to medical application of hesperidin in preventing obesity caused by high fat diet. Different feeding modes and administration treatment are applied to male BALB / c mice, it is proved that hesperidin can prevent weight gain and serum total cholesterol concentration and triglyceride concentration increase caused by high-fat diet, and it is verified that hesperidin has a good effect on stabilizing blood sugar through glucose tolerance and insulin tolerance tests, and the hesperidin can be used for preventing and treating the high-fat diet. The insulin resistance can be effectively relieved. A new selection thought is provided for prevention and treatment of obesity and various complications caused by high-fat or high-calorie diet commonly existing at present.

Description

Technical Field

[0001] The invention discloses a new use of hesperidin in preventing obesity caused by bad eating habits, and relates to the technical field of medical pharmacy. Background Art

[0002] Obesity is a chronic metabolic disease that is prevalent in modern society. According to reports, the prevalence of obesity is continuing to rise worldwide. The number of obese or overweight people in the world accounts for more than 30% of the world's population. Obesity has become a major public health problem worldwide. The causes of obesity are complex and are caused by a variety of factors, including genetics, environment, personal diet and exercise. Bad eating habits are one of the main factors leading to obesity. When the calories consumed are more than the calories consumed, the excess calories will be stored in the body in the form of fat. When the storage exceeds the normal physiological needs of the body and reaches a certain value, it becomes obese. Obese people are more likely to suffer from diseases such as type 2 diabetes, cardiovascular disease, and hypertension, and their life span will also be shorter. Long-term obesity will also increase the risk of various cancers, Alzheimer's disease, and infertility. At present, obesity is mainly prevented and treated through lifestyle intervention and medical means. More and more studies have found that many extracts from Chinese herbal medicines are beneficial for weight loss, lowering blood lipids, and inhibiting fat accumulation, which provides new treatment options for obese patients.

[0003] Hesperidin is mainly found in the fruit of the Rutaceae plant pomelo, the peel and pulp of grapefruit, tangerine, and orange, and belongs to the flavonoids. Hesperidin has anti-inflammatory, antiviral, antioxidant and anti-cancer properties, can relieve pain, lower blood pressure, reduce the formation of thrombus, improve local microcirculation and nutrient supply, and can be used to prevent and treat cardiovascular and cerebrovascular diseases. Traditional Chinese medicine believes that pomelo is sweet and sour, cold in nature, and has the functions of strengthening the stomach and digesting food, lowering gas and eliminating phlegm, and lightening the body and pleasing the complexion. Modern medical research has found that pomelo meat contains very rich vitamin C and insulin-like ingredients, so it has the effects of lowering blood sugar, lowering blood lipids, losing weight, beautifying the skin and nourishing the face. Therefore, the present invention further verifies the effect of hesperidin in preventing and treating obesity. Summary of the invention

[0004] The present invention has found through experiments that hesperidin can effectively prevent weight gain and increased triglyceride concentration in the blood caused by a high-fat diet, can reduce insulin resistance and improve glucose tolerance, and proves that hesperidin can effectively prevent obesity caused by a poor diet.

[0005] The hesperidin of the present invention has the molecular formula: 28 H 34 O 15 , molecular weight: 610.56.

[0006] The positive effects of the present invention are: The invention provides the medical use of hesperidin in preventing obesity caused by a high-fat diet, discloses that hesperidin can reduce body weight, lower triglyceride and total cholesterol concentrations, increase glucose tolerance and insulin tolerance, thereby reducing the degree of obesity, and provides a new drug option for preventing and treating obesity. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 This is a comparative curve diagram of the effect of hesperidin on the body weight of male BALB / c mice fed with different feeding methods; Figure 2 The figure shows the effect of hesperidin on the concentration of triglycerides in the serum of male BALB / c mice fed with different feeding methods. Figure 3 The figure shows the effect of hesperidin on the total cholesterol concentration in the serum of male BALB / c mice fed with different feeding methods. Figure 4 Figure 1: Glucose tolerance test (GTT) of hesperidin on male BALB / c mice fed with different feeding methods; Figure 5 Figure 1: Insulin tolerance test (ITT) of hesperidin on male BALB / c mice fed with different feeding methods; DETAILED DESCRIPTION

[0008] The present invention is further described by way of the following examples, which do not limit the present invention in any way. Without departing from the technical solution of the present invention, any modification or change made to the present invention that is easily implemented by a person of ordinary skill in the art will fall within the scope of the claims of the present invention.

[0009] The hesperidin used in the specific embodiment of the present invention was purchased from Chengdu Derui Biotechnology Co., Ltd., with a purity of ≥98%.

[0010] Example 1 Analysis of intervention methods and main indicators in animal experiments Male BALB / c mice (6-8 weeks old) were selected and purchased from Liaoning Changsheng Biotechnology Co., Ltd. After one week of adaptive feeding, they were divided into three groups, namely, a high-fat diet group (fed with 60% high-fat food, 6 mice), a hesperidin high-fat diet group (fed with 60% high-fat food and gavaged with 80 mg / kg of hesperidin solution every day, 6 mice), and a normal diet group (fed with maintenance food, 6 mice). Mice in each group had free access to food and water. Feeding conditions: room temperature was maintained at 25±1°C, relative humidity was 45%-65%, and light and dark were rotated for 12 hours (6:00-18:00). After 12 weeks of continuous feeding, the following analysis and detection were performed on the mice in each treatment group of Example 1: 1. Weight monitoring The body weight of mice in each group was accurately weighed at a fixed time every week and recorded 13 times in total.

[0011] Results: The effects of hesperidin on the body weight of mice were as follows: Figure 1 As shown, compared with the normal group, the body weight of the mice in the high-fat diet group was significantly increased, and the body weight of the mice in the high-fat + hesperidin group was significantly decreased, indicating that oral administration of hesperidin can inhibit the weight gain of mice fed a 60% high-fat diet.

[0012] 2. Determination of serum triglyceride concentration After 12 weeks of feeding, blood was collected from the eyeball and added to an anticoagulant tube. The tube was allowed to stand at room temperature for 1 hour and centrifuged at 4000 rpm for 10 minutes to obtain serum. The kit of Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. was used for determination and the calculation was performed according to the following formula: Triglyceride content (mmol / L) = Results: The results of serum triglyceride concentration in each group of mice were as follows: Figure 2 As shown, compared with the normal group, the concentration of serum triglycerides in the high-fat diet group was significantly increased, while the level was significantly reduced after oral administration of hesperidin.

[0013] 3. Determination of total cholesterol concentration in serum After 12 weeks of feeding, blood was collected from the eyeball and added to an anticoagulant tube. The tube was allowed to stand at room temperature for 1 hour and centrifuged at 4000 rpm for 10 minutes to obtain serum. The kit of Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. was used for determination and the calculation was performed according to the following formula: Cholesterol content (mmol / L) = Results: The total cholesterol concentration in the serum of each group of mice was measured as follows: Figure 3 As shown, compared with the normal group, the serum total cholesterol concentration in the high-fat diet group was significantly increased, while the level was significantly reduced after oral administration of hesperidin.

[0014] 4. Glucose tolerance test After 12 weeks of feeding, the mice were fasted for 12 h and given glucose (2 g / kg body weight) orally. Blood was collected from the tail vein of the mice at 0, 30, 60, 90 and 120 min after glucose administration to monitor blood glucose levels, perform glucose tolerance tests and draw GTT curves.

[0015] Results: The glucose tolerance results of each group of mice were as follows Figure 4 As shown in Figure 2, an increase in blood glucose levels was observed in all groups 30 minutes after oral gavage of glucose. Compared with mice fed a 60% high-fat diet alone, hesperidin-treated mice had a faster decrease in blood glucose, indicating that hesperidin improved blood glucose regulation in mice.

[0016] 5. Insulin tolerance test After 12 weeks of feeding, the mice were fasted for 12 hours and given a single intraperitoneal injection of insulin (0.5 IU / kg body weight). Blood was collected from the tail vein of the mice at 0, 30, 60, 90 and 120 minutes after insulin administration to monitor blood glucose levels, perform insulin tolerance tests and draw ITT curves.

[0017] Results: The insulin tolerance results of each group of mice were as follows Figure 5 As shown, a decrease in blood glucose levels was observed in all groups 30 minutes after insulin injection. Compared with mice simply fed a 60% high-fat diet, the blood glucose recovery time of high-fat diet mice intervened by hesperidin was shorter, indicating that hesperidin improved the insulin tolerance of mice.

[0018] Although the present invention has been described in detail with reference to the accompanying drawings and in combination with the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily think of changes or substitutions within the technical scope disclosed by the present invention, and these shall be within the scope of protection of the present invention.

Claims

1. The medical use of hesperidin in preventing obesity caused by a high-fat diet.

2. The hesperidin according to claim 1, characterized in that The drug purity is greater than 98%, and the drug dosage form is a pharmaceutically acceptable carrier, such as injection, capsule, tablet or powder injection.

3. The medical use according to claim 1, characterized in that: Hesperidin can reduce body weight, lower triglyceride concentrations, lower total cholesterol concentrations, reduce insulin resistance, and improve glucose tolerance.

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