A compound feed additive for fattening cattle and preparation method thereof
By developing a composite feed additive containing gelatinized starchurea, guanidine acetic acid, modified diatomaceous earth and hail vegetable extract, the problem that traditional additives cannot meet the growth needs of cattle and the high cost of soybean meal is solved, and the effect of improving the growth performance and meat quality of cattle is achieved.
Patent Information
- Application Number
- CN202411774635.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-05
- Publication Date
- 2025-04-18
- Estimated Expiration
- 2044-12-05
AI Technical Summary
Traditional fattening and feed additives cannot fully meet the growth needs of cattle, and soybean meal is expensive and overused can lead to indigestion and other problems.
A composite feed additive was developed, including gelatinized starchurea, guanidineacetic acid, modified diatomaceous earth and hail vegetable extract. Through the sustained release properties of modified diatomaceous earth and the film-forming properties of hail vegetable extract, the utilization rate of nutrients in animals is improved.
This compound additive can reduce the proportion of soybean meal in the diet, reduce feeding costs, improve the growth and development ability of cattle, promote intestinal health, and improve meat quality.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of feed additives, and in particular relates to a compound feed additive for fattening cattle and a preparation method thereof. Background Art
[0002] With the development of animal husbandry, the scale of fattening cattle breeding is expanding, and the demand for feed additives is also increasing. High-quality compound feed additives can improve the growth rate, feed conversion rate and meat quality of fattening cattle, thereby increasing breeding benefits.
[0003] In order to increase the growth rate of fattening cattle, improve meat quality, enhance immunity and increase feed utilization, the research and development and application of feed additives are particularly important. In recent years, with the advancement of science and technology and the improvement of breeding technology, more and more new feed additives have been developed to meet the nutritional needs of fattening cattle at different growth stages.
[0004] Traditional feed additives for fattening cattle are mainly used to provide basic nutrition, such as protein, minerals and vitamins. However, with the deepening of research, people have found that relying solely on these basic nutrients cannot fully meet the growth needs of fattening cattle. In addition, with the development of the beef cattle industry, the market price of soybean meal, as a high-quality protein feed, has continued to rise, and its cost is higher than that of other substances. Although soybean meal has high nutritional value, excessive use may also cause some problems, such as indigestion and diarrhea.
[0005] In summary, the development of a compound feed additive for fattening cattle and a preparation method thereof are of great significance for comprehensively improving the growth performance, health status and meat quality of fattening cattle, and improving the production efficiency and economic benefits of animal husbandry. Summary of the invention
[0006] The first object of the present invention is to provide a composite feed additive for fattening cattle. The modified diatomaceous earth in the composite additive has good sustained-release performance, which can improve the performance of traditional Chinese medicine polypeptides, and the sea hail extract can improve the stability of the modified diatomaceous earth and improve the utilization rate of nutrients by animals.
[0007] In order to achieve the above object, the technical solution adopted by the present invention is:
[0008] A compound feed additive for fattening cattle, comprising the following raw materials in parts by weight: 5-10 parts of gelatinized starch urea, 1-3 parts of guanidinoacetic acid, 0.5-1 parts of modified diatomaceous earth, 0.1-0.5 parts of rumen bypass vitamin C, and 0.3-0.5 parts of sea hail extract;
[0009] The preparation process of the modified diatomite is as follows:
[0010] (1) adding diatomaceous earth to water containing a surfactant, then adding calcium chloride, stirring to react, and after the reaction is completed, filtering, washing, drying, and calcining the reaction solution to obtain pretreated diatomaceous earth;
[0011] (2) Add the diatomaceous earth pretreated in step (1) into water, then add the Chinese medicine polypeptide composition, heat and stir for a period of time, and collect the product, which is the modified diatomaceous earth.
[0012] The modified diatomaceous earth can be further treated with sodium dodecylbenzene sulfonate and calcium chloride to increase its specific surface area, improve its dispersibility and fluidity, and enhance its adsorption capacity for Chinese medicine polypeptides; the calcium ions in the calcium chloride can enter the diatomaceous earth through ion exchange, which can further provide a calcium source for feed and promote the growth and development of bulls.
[0013] Furthermore, the preparation method of the Chinese medicine polypeptide composition is: the Chinese medicine is dried and ground into powder, enzymolyzed with cellulase and papain respectively, after the enzymolysis is completed, the filtrate is filtered and collected, and the filtrate is freeze-dried to obtain the Chinese medicine polypeptide composition.
[0014] Furthermore, the surfactant in step (1) is sodium dodecylbenzene sulfonate, and the mass ratio of diatomaceous earth, sodium dodecylbenzene sulfonate and calcium chloride is 1:(0.01-0.04):(0.06-0.1).
[0015] Furthermore, the stirring reaction temperature is 80-100° C., and the stirring reaction time is 1-2 h.
[0016] Furthermore, in step (2), the mass ratio of the pretreated diatomaceous earth to the traditional Chinese medicine polypeptide composition is 1:(9-15), the heating and stirring temperature is 35-45° C., and the heating and stirring time is 1-2 h.
[0017] Furthermore, the traditional Chinese medicine is a mixture of eucommia leaves, kudzu root and tea residues in a mass ratio of 1:1:(3-5).
[0018] Furthermore, the temperature of the enzymatic hydrolysis is 40-50°C and the time is 1-2h.
[0019] Furthermore, the mass ratio of the traditional Chinese medicine, cellulase and papain is 1:(0.002~0.004):(0.001~0.002).
[0020] The second object of the present invention is to provide a method for preparing the above-mentioned composite feed additive for fattening cattle, which is simple, efficient, cost-saving and suitable for industrial production.
[0021] In order to achieve the above object, the technical solution adopted by the present invention is:
[0022] Weigh gelatinized starch urea, guanidinoacetic acid, modified diatomaceous earth, rumen bypass vitamin C, and seaweed extract according to the above weight portions, stir and mix evenly to obtain the product.
[0023] Compared with the prior art, the beneficial effects of the present invention are mainly:
[0024] 1. The present invention provides a composite feed additive for fattening cattle, the additive comprising gelatinized starch urea, guanidinoacetic acid, modified diatomaceous earth, sea hail extract and other ingredients. Through experiments, it is found that the additive is creatively added to the feed, the proportion of soybean meal in the diet is reduced, the feeding cost is reduced, and the nutritional components of the feed can be enriched, making the feed more balanced, which helps to meet the nutritional needs of animals at different growth stages, improve the growth and development ability of cattle, and promote intestinal health. The modified diatomaceous earth in the composite additive has good sustained-release properties, can effectively release Chinese medicine polypeptides, and improve the absorption effect of the substance by animals. The sea hail extract molecules have certain film-forming properties, which can improve the stability of the modified diatomaceous earth in vitro, and are easily digested and absorbed in animals, which can improve the utilization rate of nutrients by animals.
[0025] 2. The present invention provides a method for preparing the above-mentioned composite feed additive for fattening cattle. The method is simple, efficient, cost-saving, and suitable for industrial production. DETAILED DESCRIPTION
[0026] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.
[0027] Example 1
[0028] A compound feed additive for fattening cattle, comprising the following raw materials in parts by weight: 7.5 parts of gelatinized starch urea, 2 parts of guanidinoacetic acid, 0.75 parts of modified diatomaceous earth, 0.3 parts of rumen bypass vitamin C, and 0.4 parts of sea hail extract;
[0029] The preparation process of the extract of sea hail is as follows: the sea hail is cleaned, dried, crushed and sieved to obtain sea hail powder; 50g of sea hail powder is weighed and added to 1000mL of water, soaked for 20 hours, filtered after soaking, and the filtrate is centrifuged at 5000rpm for 15min to separate the supernatant; the supernatant is concentrated to 5% of the original volume, and then 2 times the volume of ethanol (95%) of the concentrate is added, alcohol precipitation is carried out for 15h, and centrifuged again at 5000rpm for 15min, the precipitate is dissolved in water, and then Sevage reagent is added for deproteinization, centrifuged at 5000rpm for 15min, and this is repeated 3 times. Finally, the sea hail extract is obtained after reduced pressure concentration and freeze-drying.
[0030] The preparation process of the modified diatomite is as follows:
[0031] (1) Add 100 g of diatomaceous earth to 1000 mL of deionized water containing 3 g of sodium dodecylbenzene sulfonate, then add 8 g of calcium chloride, heat to 90 ° C and stir to react for 1.5 h. After the reaction is completed, filter, wash and dry the reaction solution; calcine at 500 ° C for 4.5 h to obtain pretreated diatomaceous earth;
[0032] (2) Add 1 g of the diatomaceous earth pretreated in step (1) into deionized water, then add 12 g of the Chinese medicine polypeptide composition, stir at 40° C. for 1.5 h, collect the product, wash it three times with deionized water, and vacuum dry it at 45° C. for 12 h to obtain the modified diatomaceous earth.
[0033] The preparation process of the traditional Chinese medicine polypeptide composition is as follows: eucommia leaves, kudzu root and tea residues are mixed in a mass ratio of 1:1:4, dried at 45°C for 12 hours and then ground to obtain traditional Chinese medicine powder, then 8 times the total weight of water of the raw materials are added, soaked for 1 hour, cellulase and papain are added for enzymolysis at 45°C for 1.5 hours (wherein the mass ratio of the above traditional Chinese medicine powder, cellulase and papain is 1:0.003:0.0015), and after the enzymolysis is completed, the filtrate is filtered and collected, and the filtrate is freeze-dried to obtain the traditional Chinese medicine polypeptide composition.
[0034] This embodiment 1 also provides a method for preparing a compound feed additive for fattening cattle, comprising the following steps:
[0035] Weigh gelatinized starch urea, guanidinoacetic acid, modified diatomaceous earth, rumen bypass vitamin C, and seaweed extract according to the above weight portions, stir and mix evenly to obtain the product.
[0036] Example 2
[0037] A compound feed additive for fattening cattle, comprising the following raw materials in parts by weight: 5 parts of gelatinized starch urea, 1 part of guanidinoacetic acid, 0.5 parts of modified diatomaceous earth, 0.1 parts of rumen bypass vitamin C, and 0.3 parts of sea hail extract;
[0038] The preparation process of the extract of Psoralea corylifolia is the same as that of Example 1.
[0039] The preparation process of the modified diatomite is as follows:
[0040] (1) 100 g of diatomaceous earth was added to 1000 mL of deionized water containing 1 g of sodium dodecylbenzene sulfonate, and then 6 g of calcium chloride was added, and the temperature was raised to 100 ° C and stirred for reaction for 1 h. After the reaction was completed, the reaction solution was filtered, washed, dried, and calcined at 450 ° C for 5 h to obtain pretreated diatomaceous earth;
[0041] (2) Add 1 g of the diatomaceous earth pretreated in step (1) into deionized water, then add 9 g of the Chinese medicine polypeptide composition, stir at 35° C. for 2 h, collect the product, wash it three times with deionized water, and vacuum dry it at 45° C. for 12 h to obtain the modified diatomaceous earth.
[0042] The preparation process of the traditional Chinese medicine polypeptide composition is as follows: eucommia leaves, kudzu root and tea residues are mixed in a mass ratio of 1:1:3, dried at 45°C for 12 hours and then ground to obtain traditional Chinese medicine powder, then 8 times the total weight of water of the raw materials are added, soaked for 1 hour, cellulase and papain are added for enzymolysis at 40°C for 2 hours (wherein the mass ratio of the traditional Chinese medicine powder, cellulase and papain is 1:0.002:0.001), and after the enzymolysis is completed, the filtrate is filtered and collected, and the filtrate is freeze-dried to obtain the traditional Chinese medicine polypeptide composition.
[0043] This embodiment 1 also provides a method for preparing a compound feed additive for fattening cattle, comprising the following steps:
[0044] Weigh gelatinized starch urea, guanidinoacetic acid, modified diatomaceous earth, rumen bypass vitamin C, and seaweed extract according to the above weight portions, stir and mix evenly to obtain the product.
[0045] Example 3
[0046] A compound feed additive for fattening cattle, comprising the following raw materials in parts by weight: 10 parts of gelatinized starch urea, 3 parts of guanidinoacetic acid, 1 part of modified diatomaceous earth, 0.5 parts of rumen bypass vitamin C, and 0.5 parts of sea hail extract;
[0047] The preparation process of the extract of Psoralea corylifolia is the same as that of Example 1.
[0048] The preparation process of the modified diatomite is as follows:
[0049] (1) Add 100 g of diatomaceous earth to 1000 mL of deionized water containing 4 g of sodium dodecylbenzene sulfonate, then add 10 g of calcium chloride, heat to 80 ° C and stir to react for 2 h. After the reaction is completed, filter, wash, and dry the reaction solution, and calcine at 550 ° C for 4 h to obtain pretreated diatomaceous earth;
[0050] (2) Add 1 g of the diatomaceous earth pretreated in step (1) into deionized water, then add 15 g of the Chinese medicine polypeptide composition, heat to 45° C. and stir for 1 h, collect the product, wash it 3 times with deionized water, and vacuum dry it at 45° C. for 12 h to obtain the modified diatomaceous earth.
[0051] The preparation process of the traditional Chinese medicine polypeptide composition is as follows: eucommia leaves, kudzu root and tea residues are mixed in a mass ratio of 1:1:5, dried at 45°C for 12 hours and then ground to obtain traditional Chinese medicine powder, then 8 times the total weight of water of the raw materials are added, soaked for 1 hour, cellulase and papain are added for enzymolysis at 50°C for 1 hour (wherein the mass ratio of the traditional Chinese medicine powder, cellulase and papain is 1:0.004:0.002), and after the enzymolysis is completed, the filtrate is filtered and collected, and the filtrate is freeze-dried to obtain the traditional Chinese medicine polypeptide composition.
[0052] This embodiment 1 also provides a method for preparing a compound feed additive for fattening cattle, comprising the following steps:
[0053] Weigh gelatinized starch urea, guanidinoacetic acid, modified diatomaceous earth, rumen bypass vitamin C, and seaweed extract according to the above weight portions, stir and mix evenly to obtain the product.
[0054] Comparative Example 1
[0055] This comparative example provides a compound feed additive for fattening cattle, which differs from Example 1 in that the modified diatomaceous earth is replaced by diatomaceous earth and a traditional Chinese medicine polypeptide composition, wherein the preparation method of the traditional Chinese medicine polypeptide composition is the same as that of Example 1.
[0056] Comparative Example 2
[0057] This comparative example provides a compound feed additive for fattening cattle, which is different from Example 1 in that the Chinese medicine polypeptide composition is replaced by a Chinese medicine extract, wherein the preparation process of the Chinese medicine extract is as follows: Eucommia leaves, Pueraria root, and tea residues are mixed in a mass ratio of 1:1:4, dried at 45°C for 12 hours, and then ground to obtain Chinese medicine powder, and then 8 times the total weight of water of the raw materials is added, soaked for 1 hour, boiled at 90°C for 1.5 hours, and then filtered, concentrated, and dried to obtain the Chinese medicine extract.
[0058] Comparative Example 3
[0059] This comparative example provides a compound feed additive for fattening cattle, which is different from Example 1 in that the extract of the sea hail grass is omitted.
[0060] The following is the effect test of the compound feed obtained in Examples 1-3 and Comparative Examples 1-3.
[0061] Test Example 1
[0062] 11-month-old, healthy Simmental bulls were selected and divided into a control group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Example 1 group: 0.9% of the compound feed additive obtained in Example 1 was added to the diet. The difference between the control group and Example 1 is that the additive of Example 1 was not added during the feeding process, and the rest was the same as Example 1; the additives of the corresponding groups were added to the diet of the remaining groups (0.9% of the basic diet amount). The experimental cattle were all raised in free stalls, and the feeding area for each cattle was 14m 2 , free drinking water, free movement, feeding twice a day (07:00, 16:00), record the daily feed intake of each cow. Each group of cows transitioned to the experimental diet during the pre-feeding period, and the transition period was 15 days.
[0063] In order to study the effect of the feed additive of the present invention on the growth performance of fattened cattle, the fasting weight of each group of cattle in Examples 1 to 3 and Comparative Examples 1 to 3 before morning feeding was tested on the 1st day and 100th day of the test period, and the daily individual feed intake of each group of cattle was measured by a feed intake measuring device (Beijing Huiqi Tongda IoT Technology). The dry matter intake (DMI), average daily weight gain (ADG) and feed-to-weight ratio (F / G) of each cattle were calculated. The test results are shown in Table 1.
[0064]
[0065] In the fattening and breeding production of Simmental cattle, daily weight gain is an important indicator reflecting the fattening effect of Simmental cattle. The mass of dry matter consumed by average daily weight gain, i.e., feed-to-weight ratio (F / G), is a key indicator for measuring the feed cost per kilogram of weight gain of beef cattle. It can be seen from the test results in Table 1 that the fattening and weight gain performance of Simmental bulls in Examples 1 to 3 of the present invention is improved.
[0066] In this experiment, the compound feed additive described in Example 1 was used to replace the soybean meal in the diet. It can be seen that during the entire feeding cycle, the total weight gain, average daily weight gain, and feed-to-weight ratio of the Simmental fattening bulls in groups 1 to 3 of Examples were significantly improved compared with those in groups 1 to 3 of Comparative Examples, indicating that replacing soybean meal with the compound feed additive described in Example 1 failed to promote the growth of cattle.
[0067] Compared with Example 1, in Comparative Example 1, the modified diatomite was replaced with a diatomite and Chinese medicine polypeptide composition, and the diatomite was not modified. The total weight gain and average daily weight gain of Simmental fattening bulls were both reduced, and the feed-to-weight ratio was increased. On the one hand, it may be because the sodium dodecylbenzene sulfonate added during the diatomite modification process can improve the dispersion ability of diatomite, increase the specific surface area of diatomite, and improve the loading capacity of diatomite for polypeptides. On the other hand, it is because the calcium chloride added in the process can also provide a calcium source for animals, which helps to improve the growth and development ability of cattle.
[0068] Compared with Example 1, in Comparative Example 2, the Chinese medicine polypeptide was replaced with a Chinese medicine extract, and the total weight gain and average daily weight gain of the Simmental fattening bulls in this group were reduced. This result illustrates the importance of the Chinese medicine polypeptide of the present invention in promoting the growth and development of cattle. Compared with Example 1, the total weight gain and average daily weight gain of the Simmental fattening bulls in Comparative Example 3 were also reduced, and the feed-to-weight ratio was increased. This is because the addition of the sea hail extract was omitted. The sea hail extract has a certain film-forming property, which can improve the stability of the modified diatomaceous earth in vitro; it is easily digested and absorbed in the animal body, which can improve the utilization rate of nutrients by animals.
[0069] Test Example 2
[0070] The cattle were slaughtered 110 days after the test, and the live weight before slaughter, carcass weight and slaughter rate of Examples 1 to 3 and Comparative Examples 1 to 3 were measured.
[0071] Live weight before slaughter: The weight of the test cattle measured before slaughter.
[0072] Carcass weight: The remaining trunk after slaughter and bleeding, minus the head, hooves (the part below the front knee joint and the toe joint of the hind limbs), skin, viscera, tail, reproductive organs, kidneys and kidney fat.
[0073] Slaughter rate (%): (carcass weight / live weight before slaughter) × 100.
[0074] The above test results are recorded in Table 2.
[0075]
[0076] It can be seen from the test results in Table 2 that the slaughter rates of cattle in Examples 1 to 3 are significantly improved compared with those in Comparative Examples 1 to 3, which indicates that the feed of the present invention can reduce feeding costs and improve the growth and development ability of cattle by reducing the proportion of soybean meal in the diet.
[0077] Compared with Example 1, the comparative example 1 group did not modify the diatomite, but simply compounded it with the Chinese medicine polypeptide composition, and the slaughter rate also decreased. This is because the modified diatomite has good sustained-release properties, which can improve the effect of the Chinese medicine polypeptide and increase the slaughter rate. The slaughter rate of comparative example 2 decreased most significantly, indicating that the Chinese medicine polypeptide of the present invention can significantly increase the slaughter rate; the slaughter rate of comparative example 3 decreased to 59.32%, because the addition of the sea hail extract can improve the stability of the modified diatomite and improve the utilization rate of nutrients by animals, thereby effectively improving the slaughter rate of cattle.
[0078] Test Example 3
[0079] The cattle were slaughtered 110 days after the test, and the quality of the beef described in Examples 1 to 3 of the present invention and Comparative Examples 1 to 3 was measured.
[0080] (1) Determination of cooking loss: weigh the sample meat block as W1, put it into a cooking bag and heat it in a water bath at 80℃. When the center temperature of the meat sample reaches 70℃, take out the meat block and cool it to room temperature. Weigh the meat sample again as W2. Cooking loss (%) = (W1-W2) / W1×100;
[0081] (2) Shear force measurement: measured in accordance with NY / T 1180-2006 standard;
[0082] (3) Determination of drip loss: carried out in accordance with NY / T 1333-2007, the method for determining the quality of livestock and poultry meat;
[0083] (4) Meat color value: Select three measurement points on the front and back of the meat sample (evenly distributed on the longest diagonal line), and measure each measurement point three times using a colorimeter. The average value of the three times is recorded as the final L, a, and b values. The determination of moisture, protein, and fat is carried out according to the corresponding determination methods in the national standard.
[0084]
[0085] The drip loss and cooking loss indicate the water-binding capacity of beef, that is, the water-retaining capacity of beef. The flavor substances and taste of beef are closely related to the water-binding capacity. The smaller the drip loss and cooking loss, the better the water-retaining performance of the meat. The test results in Table 3 show that the water-retaining performance of the beef in Examples 1 to 3 is not significantly affected, while the water-retaining performance of the beef in Comparative Examples 1 to 3 is reduced.
[0086] Shear force is an indicator that can directly reflect the tenderness of beef. From the test results in Table 3, it can be seen that the shear force of the beef in Examples 1 to 3 of the present invention can be significantly improved, and the tenderness is increased.
[0087] Meat color plays a key role in evaluating the quality of beef cattle. The brightness value L, redness value a, and yellowness value b directly affect the brightness and freshness of beef. It can be seen that the beef of Examples 1 to 3 groups presents a lustrous red color, and the brightness value, redness value, and yellowness value are all improved.
[0088] Observation of the changes in the moisture, protein and fat content of beef in the above groups showed that adding compound feed additives to the diet instead of soybean meal slightly increased the fat and protein content in the muscles of Simmental fattening bulls.
[0089] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.
Claims
1. A compound feed additive for fattening cattle, characterized in that: The method comprises the following raw materials in parts by weight: 5-10 parts of gelatinized starch urea, 1-3 parts of guanidinoacetic acid, 0.5-1 parts of modified diatomaceous earth, 0.1-0.5 parts of rumen bypass vitamin C, and 0.3-0.5 parts of sea hail extract; The preparation process of the modified diatomite is as follows: (1) adding diatomaceous earth to water containing a surfactant, then adding calcium chloride, stirring to react, and after the reaction is completed, filtering, washing, drying, and calcining the reaction solution to obtain pretreated diatomaceous earth; (2) adding the diatomaceous earth pretreated in step (1) into water, then adding the traditional Chinese medicine polypeptide composition, heating and stirring for a period of time, and collecting the product, which is the modified diatomaceous earth; The surfactant in step (1) is sodium dodecylbenzene sulfonate, and the mass ratio of diatomaceous earth, sodium dodecylbenzene sulfonate and calcium chloride is 1:(0.01-0.04):(0.06-0.1); The preparation method of the traditional Chinese medicine polypeptide composition comprises: drying the traditional Chinese medicine and grinding it into powder, respectively performing enzymolysis with cellulase and papain, filtering and collecting the filtrate after the enzymolysis, and freeze-drying the filtrate to obtain the traditional Chinese medicine polypeptide composition; the traditional Chinese medicine is a mixture of eucommia leaves, kudzu root, and tea residues in a mass ratio of 1:1:(3-5); The preparation process of the extract of sea hail is as follows: the sea hail is cleaned, dried, crushed and sieved to obtain sea hail powder; 50g of sea hail powder is weighed and added into 1000mL of water, soaked for 20h, filtered after soaking, and the filtrate is centrifuged to separate the supernatant; the supernatant is concentrated to 5% of the original volume, and then 2 times the volume of 95% ethanol of the concentrate is added, and the alcohol precipitation is carried out for 15h, and centrifuged again, and the obtained precipitate is dissolved in water, and then Sevage reagent is added to deproteinize, and centrifuged, and this is repeated 3 times, and finally the sea hail extract is obtained after reduced pressure concentration and freeze-drying.
2. The compound feed additive for fattening cattle according to claim 1, characterized in that: The stirring reaction temperature is 80-100° C., and the stirring reaction time is 1-2 h.
3. The compound feed additive for fattening cattle according to claim 1, characterized in that: In step (2), the mass ratio of the pretreated diatomaceous earth to the traditional Chinese medicine polypeptide composition is 1:(9-15), the heating and stirring temperature is 35-45° C., and the heating and stirring time is 1-2 h.
4. The compound feed additive for fattening cattle according to claim 1, characterized in that: The temperature of the enzymolysis is 40-50° C., and the time is 1-2 hours.
5. The compound feed additive for fattening cattle according to claim 1, characterized in that: The mass ratio of the traditional Chinese medicine, cellulase and papain is 1:(0.002-0.004):(0.001-0.002).
6. The method for preparing the compound feed additive for fattening cattle according to claim 1, characterized in that: The method comprises the following steps: weighing gelatinized starch urea, guanidine acetic acid, modified diatomaceous earth, rumen bypass vitamin C and sea hail extract according to the weight proportions of claim 1, stirring and mixing evenly to obtain the product.
Citation Information
Patent Citations
Compound feed product containing folium eucommiae
CN107647108A
Chinese herbal medicine compound feed additive for fattening beef cattle
CN107647122A