Method for preventing and controlling weeds in cowslip propagation field

By spraying compound pesticides at the three-leaf stage of *Gnaphalium affine* and combining this with soil sealing and seed treatment, the problem of weed control in *Gnaphalium affine* seed production fields was solved, achieving efficient weed control and ensuring the success of *Gnaphalium affine* planting and growth.

CN119256892BActive Publication Date: 2026-03-24盐池县科技服务中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-30
Publication Date
2026-03-24

AI Technical Summary

Technical Problem

There is a lack of effective weed control methods in existing technology for seed production fields of *Gnaphalium affine*, especially in Ningxia. Improper use of chemical herbicides may lead to crop damage and weed resistance. There is a lack of one or more compound pesticides with better weed control effects in seed production fields of *Gnaphalium affine*.

Method used

The method of first spraying the stems and leaves of *Gnaphalium affine* at the three-leaf stage was adopted, which included a combination of 5% imidacloprid, 158 g/L high-efficiency flupyridine, and 440 g/L flufenoxuron·benzon, combined with pre-sowing soil sealing, seed treatment such as mechanical compaction and lentinan soaking, and supplementary application of pesticides according to the growth status of *Gnaphalium affine*.

Benefits of technology

It achieves efficient control of weeds in seedbeds of *Gynostemma pentaphyllum*, ensuring successful seedling emergence and survival, improving seed germination rate and herbicide resistance, reducing fresh weight of weeds, and is suitable for large-scale planting in Ningxia.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application discloses a method for controlling weeds in a cowslip tree breeding field, which comprises the step of spraying a compound pesticide on stems and leaves for the first time at the three-leaf stage of the cowslip tree, wherein the compound pesticide and the dosage are 150-210 mL / mu of 5% imidazolinyl acetic acid, 50-70 mL / mu of 158 g / L high-efficiency haloxyfop- r-methyl and 50-70 mL / mu of 440 g / L fluometuron-molinate. The method has a better effect on controlling weeds in the cowslip tree breeding field, and provides integrated technical support for preventing and controlling weeds in large-area cowslip tree planting production.
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Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant cultivation. More particularly, the present application relates to a method for controlling weeds in a Lespedeza potaninii breeding field. BACKGROUND

[0002] Lespedeza potaninii, also known as Lespedeza potaninii, is a plant of the genus Lespedeza in the family Fabaceae. It is widely distributed in desert grasslands and arid grasslands. Lespedeza potaninii is the most drought-resistant species in the genus Lespedeza. It can survive in arid regions with annual precipitation of less than 150 mm. It is a dominant low shrub species in the desert grasslands of Xinjiang, Inner Mongolia, and Ningxia. Lespedeza potaninii is a good legume forage grass that is preferred by cattle and sheep in grassland areas. It is one of the main forage grass species in pastoral areas. From the perspective of biodiversity, ecosystem multifunctionality and multiservice, and ecosystem stability, Lespedeza potaninii is an important choice for near-natural restoration of severely degraded desert grasslands. The biggest bottleneck in near-natural restoration is the lack of or very few local superior grass species. The ecological restoration of severely degraded grasslands in Yanchi, Ningxia, also faces the same problem. Research on weed control technology in breeding fields is particularly urgent and important as an important component of Lespedeza potaninii breeding integrated technology.

[0003] At present, the weed control mainly adopts chemical weeding measures, the chemical weeding can effectively solve the harm of weeds to crops, greatly reduces the weeding cost and labor cost of agricultural production, and is conducive to promoting the development of agricultural production. The herbicide is through the physiological and biochemical effect of plant absorption, the non-target resistance of weeds to herbicide exists, including herbicide absorption, translocation, storage and metabolism, and then interferes with the metabolism of plants to cause death, the herbicide is divided into selective herbicide, total herbicide, systemic herbicide, contact type herbicide, stem leaf treatment agent, soil treatment agent and the like according to different classification methods, wherein the soil sealing treatment before seedling is the key of chemical weeding, which can obviously reduce the occurrence base number of weeds in the field, effectively reduce the pressure of stem and leaf spraying weeding in the later period, however, improper selection and application of herbicide and use technical problems will cause serious phytotoxicity of herbicide to crops, and the use of long residual period of herbicide will also harm the production of subsequent crops, causing loss, and the growth is inhibited, the yield is reduced, the seedlings are dead, and the yield is reduced, during the planting period of the cow branch, including several important periods: (1) seedling stage: the period when two true leaves completely unfold; (2) three-leaf stage: the first compound leaf completely unfolds; (3) five-leaf stage: the fourth and fifth leaves of the second compound leaf completely unfold, and the sixth leaf is small, the weed control means and effect are different in different growth periods of the cow branch. The effect of weed control is mainly investigated by the total number of surviving weeds and the fresh weight control effect, wherein the total number of surviving weeds is investigated, which reflects the killing effect of the pesticide on weeds, and the weight of surviving weeds is investigated, which reflects the growth inhibition effect of the pesticide on weeds. Through multiple ways, it is found that no scholar has done the research on the integrated technology of weed control in the cow branch propagation field at present, and there is lack of one or more compound pesticide with better weed control effect in the cow branch propagation field. SUMMARY

[0004] An object of the present application is to solve at least the above problems and to provide at least the advantages to be described later.

[0005] Another object of the present application is to provide a weed control method for cow branch propagation field, which has better weed control effect in the cow branch propagation field, and provides integrated technical support for weed control for large-scale planting of cow branch.

[0006] In order to achieve these objects and other advantages according to the present application, a weed control method for cow branch propagation field is provided, which comprises the step of spraying compound pesticide on the stem and leaf for the first time at the three-leaf stage of the cow branch, the compound pesticide and the amount are 5% imidazolinone 150-210 mL / acre, 158 g / L high-efficiency fluazifop-butyl 50-70 mL / acre and 440 g / L fluometuron·linuron 50-70 mL / acre, wherein the fluometuron·linuron includes 50 g / L trifluron and 360 g / L linuron.

[0007] Preferably, the complex pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L flumipropyn 60 mL / acre and 440 g / L fluometuron + benthiocarb 60 mL / acre.

[0008] Preferably, the method for controlling weeds in the cowslip seed multiplication field further comprises the step of soil sealing before sowing, specifically: watering the soil before sowing the cowslip seeds, applying 5% imazamox 100-140 mL / acre after watering, and then sowing after the surface is suitable for sowing.

[0009] Preferably, the method for controlling weeds in the cowslip seed multiplication field further comprises the step of treating the cowslip seeds, specifically: evenly rolling the cowslip seeds 2-4 times.

[0010] Preferably, the method for controlling weeds in the cowslip seed multiplication field further comprises the step of soaking the rolled cowslip seeds in a lentinan solution of 0.01-0.2 mg / mL for 10-30 min.

[0011] Preferably, the method for controlling weeds in the cowslip seed multiplication field further comprises the steps of watering 20-25 days after the first application of the complex pesticide, and then applying one or more of 5% imazamox, 158 g / L flumipropyn and 440 g / L fluometuron + benthiocarb 15-20 days after watering.

[0012] When the average height of the enclosed cowslip is less than 2.5 cm:

[0013] If the weed control effect is less than 85%, 5% imazamox 100-140 mL / acre, 158 g / L flumipropyn 10-30 mL / acre and 440 g / L fluometuron + benthiocarb 10-30 mL / acre are supplemented;

[0014] If the weed control effect is 85% to less than 88%, 5% imazamox 100-140 mL / acre and 158 g / L flumipropyn 10-30 mL / acre are supplemented;

[0015] If the weed control effect is 88% or more, 5% imazamox 100-140 mL / acre is supplemented;

[0016] When the average height of the enclosed cowslip is 2.5 cm to less than 3.0 cm:

[0017] If the weed control effect is less than 85%, 5% imazamox 50-70 mL / acre, 158 g / L flumipropyn 10-30 mL / acre and 440 g / L fluometuron + benthiocarb 10-30 mL / acre are supplemented;

[0018] If the weed plant control effect is 85%≤< 88%, 5% imazamox 50-70 mL / acre and 158 g / L fluroxypyr 10-30 mL / acre are additionally applied;

[0019] If the weed plant control effect is ≥ 88%, 5% imazamox 50-70 mL / acre is additionally applied;

[0020] When the average plant height of the enclosed locoweed is ≥ 3.0 cm:

[0021] If the weed plant control effect is < 85%, 5% imazamox 20-40 mL / acre, 158 g / L fluroxypyr 10-30 mL / acre and 440 g / L fluvoxyprofen · benthiocarb 10-30 mL / acre are additionally applied;

[0022] If the weed plant control effect is 85%≤< 88%, 5% imazamox 20-40 mL / acre and 158 g / L fluroxypyr 10-30 mL / acre are additionally applied;

[0023] If the weed plant control effect is ≥ 88%, 5% imazamox 20-40 mL / acre is additionally applied.

[0024] In the above preferred technical solutions, the locoweed propagation field weed control method further comprises irrigating 20-25 days after the first time of spraying the compound pesticide to cooperate with the water and fertilizer management measures of the locoweed, and new weeds are generated in the locoweed propagation field 15-20 days after the irrigation, but the overall weed influence is far lower than that in the first time of spraying the compound pesticide, because the overall pesticide amount of the additional pesticide application is lower than that of the first pesticide application, and the growth state of the locoweed and the weed control effect of the first time of spraying the compound pesticide need to be fully considered in the process of the additional pesticide application, wherein the growth state of the locoweed is a priority influencing factor, through experiments, 5% imazamox has the smallest influence on the plant height of the locoweed, the lower the plant height of the locoweed, the greater the proportion of 5% imazamox in the compound pesticide of the additional pesticide application, and with the improvement of the weed control effect of the first time of spraying the compound pesticide, the types of the compound pesticide of the additional pesticide application are gradually reduced, when the weed plant control effect is ≥ 88% after the first time of spraying the compound pesticide, only 5% imazamox is used for the additional pesticide application, when 85%≤ the weed plant control effect < 88% after the first time of spraying the compound pesticide, 5% imazamox and fluroxypyr are used for the additional pesticide application, and when the weed plant control effect < 85% after the first time of spraying the compound pesticide, 5% imazamox, fluroxypyr and fluvoxyprofen · benthiocarb are used for the additional pesticide application.

[0025] The present application at least includes the following beneficial effects:

[0026] Firstly, the application provides a weed control method for cow vetch propagation field for the first time, which is suitable for cow vetch propagation field planting in Ningxia area, effectively ensures the smooth emergence and seedling survival of cow vetch propagation field, and lays a foundation for large-area planting of cow vetch production;

[0027] Secondly, the weed control method for cow vetch propagation field provided by the application comprises the steps of soil sealing, seed treatment and stem and leaf treatment, wherein 5% imazamox is selected as an efficient herbicide for soil sealing of cow vetch propagation field in the planting year, which not only ensures the low seedling mortality rate of cow vetch, but also ensures the fresh weight control effect of weeds in cow vetch propagation field.

[0028] Thirdly, the seed treatment in the weed control method for cow vetch propagation field comprises a mechanical rolling step and a lentinan soaking step, wherein the mechanical rolling step not only removes the seed coat of cow vetch seeds, but also destroys 10-20% of the wax layer on the surface of the cow vetch seeds, so that the cow vetch seeds can normally absorb water and nutrients in the soil during germination, and the germination rate of the cow vetch seeds is improved; the application first proposes to soak the rolled cow vetch seeds with lentinan, so that the seeds have good drug resistance and disease resistance after germination, and lays a foundation for further stem and leaf treatment.

[0029] Fourthly, the weed control method for cow vetch propagation field provided by the application selects a compound pesticide for stem and leaf treatment, and the compound pesticide is 5% imazamox, 158g / L high-efficiency fluazifop-butyl and 440g / L fluometuron·butachlor, wherein the preferred weight ratio of 5% imazamox, 158g / L high-efficiency fluazifop-butyl and 440g / L fluometuron·butachlor is 3:1:1, and the application period of the compound pesticide is the three-leaf stage of cow vetch; the selected compound pesticide has the advantages of fast weed killing, strong internal absorption, high safety to cow vetch, high fresh weight control effect on weeds and low cost.

[0030] Fifthly, the weed control method for cow vetch propagation field further comprises a supplementary application step, which comprises the following steps: 20-25 days after the stem and leaf treatment of cow vetch, the soil is irrigated, and the characteristics of fertile soil but less rainfall in Ningxia area are taken into account; the weeds growing after irrigation are supplemented with the application of the compound pesticide; the application further provides scientific types and dosages of the supplementary application of the compound pesticide in combination with the plant height of cow vetch and the effect of the first application.

[0031] Sixthly, the weed control method for cow vetch propagation field can effectively prevent the damage of parasitic weeds, and the fresh weight control effect on weeds can reach more than 90% after 30 days of the first application, and there is no significant difference.

[0032] Other advantages, objects and features of the application will be embodied in part through the following description, and will be understood by those skilled in the art through research and practice of the application. Detailed Implementation

[0033] The present invention will now be described in further detail with reference to specific embodiments, so that those skilled in the art can implement it based on the description.

[0034] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0035] Selected experimental site: The experimental site is located in Sidunzi Science and Technology Innovation Base, Yanchi County, Ningxia Hui Autonomous Region, with geographical coordinates of 37°46'N, 107°28'E. The experimental site is divided into plots, each with an area of ​​24m². 2 During the experiment, the field fertilizer and water management measures remained consistent.

[0036] The experimental site is located in Yanchi County, a region with low rainfall and an agro-pastoral transition zone. The average annual rainfall is 282.3 mm, with most of the precipitation concentrated in July to September, accounting for more than 64% of the annual rainfall. The annual evaporation reaches 2131.7 mm. The absolute frost-free period is about 124 days, the relative frost-free period is 165 days, the annual accumulated temperature ≥0℃ is 3811.7℃, the annual accumulated temperature ≥10℃ is 3436.06℃, the annual sunshine duration is 2346.82 hours, and the annual average temperature is 9.1℃.

[0037] Experimental materials: imidacloprid (purchased from Shenyang Kechuang Chemicals Co., Ltd.); haloxyfop-methyl (purchased from Shenyang Hetian Chemical Co., Ltd.); flufenoxuron-methyl (purchased from Hefei Xingyu Chemical Co., Ltd.); metolachlor (purchased from Hangzhou Yingtai Biotechnology Co., Ltd.); metolachlor-methyl (purchased from Syngenta (Suzhou) Crop Protection Co., Ltd.); trifluralin (purchased from Shandong Shengbang Luye Chemical Co., Ltd.); terbufos-methyl (purchased from Jiangxi Dunpai Chemical Co., Ltd.); quizalofop-p-ethyl (purchased from Hefei Xingyu Chemical Co., Ltd.); ethoxyfluralin (purchased from Qingdao Modern Agriculture Co., Ltd.); triclopyralid (purchased from Jilin Yanbian Tianbao Biological Agent Co., Ltd.).

[0038] Example 1

[0039] Three experimental plots were randomly selected for planting *Gnaphalium affine*. Weed control methods in the seed production fields included the first foliage spraying of compound pesticides at the three-leaf stage of *Gnaphalium affine*. The compound pesticides and dosages were 180 mL / mu of 5% imidacloprid ethionyl, 60 mL / mu of 158 g / L high-efficiency fluroxypyr haloxyfop-R-methyl, and 60 mL / mu of 440 g / L flufenoxuron·benzanol. No control measures were taken at other times during the planting period of *Gnaphalium affine*.

[0040] Example 2

[0041] Randomly select 3 test plots for planting Millettia pachynema, and the weed control method in Millettia pachynema breeding field includes spraying the first time on the stems and leaves at the three-leaf stage of Millettia pachynema, and the compound pesticide and the dosage are 5% imazamox 150 mL / acre, 158 g / L high-efficiency fluazifop-butyl 50 mL / acre and 440 g / L fluometuron·basmati 50 mL / acre, and no control measures are taken at other times during the planting period of Millettia pachynema.

[0042] Example 3

[0043] Randomly select 3 test plots for planting Millettia pachynema, and the weed control method in Millettia pachynema breeding field includes:

[0044] Step one, soil sealing: after watering the soil of the test plot for 4 days, 5% imazamox 150 mL / acre is applied, and the test plot is suitable for planting;

[0045] Step two, stem and leaf treatment: the first time spraying compound pesticide on the stems and leaves at the three-leaf stage of Millettia pachynema, the compound pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre and 440 g / L fluometuron·basmati 60 mL / acre;

[0046] No control measures are taken at other times during the planting period of Millettia pachynema.

[0047] Example 4

[0048] Randomly select 3 test plots for planting Millettia pachynema, and the weed control method in Millettia pachynema breeding field includes:

[0049] Step one, soil sealing: after watering the soil of the test plot for 4 days, 5% imazamox 150 mL / acre is applied, and the test plot is suitable for planting;

[0050] Step two, Millettia pachynema seed treatment: wild Millettia pachynema is used as a cultivated variety, and the wild Millettia pachynema seeds are mechanically rolled 3 times, at which time the wild Millettia pachynema seeds are heated but not scalded, not only the seed coat of the Millettia pachynema seeds is removed, but also 10-20% of the wax layer on the surface of the Millettia pachynema seeds is destroyed;

[0051] Step three, stem and leaf treatment: the first time spraying compound pesticide on the stems and leaves at the three-leaf stage of Millettia pachynema, the compound pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre and 440 g / L fluometuron·basmati 60 mL / acre;

[0052] No control measures are taken at other times during the planting period of Millettia pachynema.

[0053] Example 5

[0054] Randomly select 3 test plots for planting of Millettia pachynema, and the weed control method in the breeding field of Millettia pachynema comprises:

[0055] Step one, soil sealing: after irrigating the soil of the test plot for 4 days, 5% imazamox 150 mL / acre is applied, and the surface of the test plot is suitable for seeding;

[0056] Step two, Millettia pachynema seed treatment: wild Millettia pachynema is used as a cultivated variety, and the wild Millettia pachynema seeds are mechanically rolled 3 times, at which time the wild Millettia pachynema seeds are heated but not scalded, not only the seed coat of the Millettia pachynema seeds is removed, but also 10-20% of the wax layer on the surface of the Millettia pachynema seeds is damaged; further, the rolled Millettia pachynema seeds are soaked in a 0.1 mg / mL lentinan solution for 20 min;

[0057] Step three, stem and leaf treatment: at the three-leaf stage of Millettia pachynema, a compound pesticide is sprayed on the stems and leaves for the first time, and the compound pesticide and the amount used are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre, and 440 g / L fluometuron·linuron 60 mL / acre;

[0058] No other control measures are taken during the planting period of Millettia pachynema.

[0059] Example 6

[0060] Randomly select 3 test plots for planting of Millettia pachynema, and the weed control method in the breeding field of Millettia pachynema comprises:

[0061] Step one, soil sealing: after irrigating the soil of the test plot for 4 days, 5% imazamox 150 mL / acre is applied, and the surface of the test plot is suitable for seeding;

[0062] Step two, Millettia pachynema seed treatment: wild Millettia pachynema is used as a cultivated variety, and the wild Millettia pachynema seeds are mechanically rolled 3 times, at which time the wild Millettia pachynema seeds are heated but not scalded, not only the seed coat of the Millettia pachynema seeds is removed, but also 10-20% of the wax layer on the surface of the Millettia pachynema seeds is damaged; further, the rolled Millettia pachynema seeds are soaked in a 0.1 mg / mL lentinan solution for 20 min;

[0063] Step three, stem and leaf treatment: at the three-leaf stage of Millettia pachynema, a compound pesticide is sprayed on the stems and leaves for the first time, and the compound pesticide and the amount used are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre, and 440 g / L fluometuron·linuron 60 mL / acre;

[0064] Step four, irrigation and supplementary pesticide application: irrigate 20-25 days after the first time of spraying the compound pesticide, and 20 days after irrigation, one or more of 5% imazamox, 158 g / L high-efficiency fluazifop-butyl, and 440 g / L fluometuron·linuron is supplemented and applied, specifically:

[0065] When the average height of the Vicia cracca in the enclosure is < 2.5 cm:

[0066] If the control effect on weed plants is < 85%, 5% imazamox 100-140 mL / acre, 158 g / L flumioxazin 10-30 mL / acre, and 440 g / L fluazifop-butyl 10-30 mL / acre are additionally applied;

[0067] If 85%≤ the control effect on weed plants < 88%, 5% imazamox 100-140 mL / acre and 158 g / L flumioxazin 10-30 mL / acre are additionally applied;

[0068] If the control effect on weed plants is ≥ 88%, 5% imazamox 100-140 mL / acre is additionally applied;

[0069] When the average height of the Vicia cracca in the enclosure is 2.5 cm < 3.0 cm:

[0070] If the control effect on weed plants is < 85%, 5% imazamox 50-70 mL / acre, 158 g / L flumioxazin 10-30 mL / acre, and 440 g / L fluazifop-butyl 10-30 mL / acre are additionally applied;

[0071] If 85%≤ the control effect on weed plants < 88%, 5% imazamox 50-70 mL / acre and 158 g / L flumioxazin 10-30 mL / acre are additionally applied;

[0072] If the control effect on weed plants is ≥ 88%, 5% imazamox 50-70 mL / acre is additionally applied;

[0073] When the average height of the Vicia cracca in the enclosure is ≥ 3.0 cm:

[0074] If the control effect on weed plants is < 85%, 5% imazamox 20-40 mL / acre, 158 g / L flumioxazin 10-30 mL / acre, and 440 g / L fluazifop-butyl 10-30 mL / acre are additionally applied;

[0075] If 85%≤ the control effect on weed plants < 88%, 5% imazamox 20-40 mL / acre and 158 g / L flumioxazin 10-30 mL / acre are additionally applied;

[0076] If the control effect on weed plants is ≥ 88%, 5% imazamox 20-40 mL / acre is additionally applied;

[0077] No control measures are taken at other times during the Vicia cracca planting period.

[0078] Comparative Example 1

[0079] Randomly select 3 test plots for planting of Millettia pachynema, and no control measures are taken for planting of Millettia pachynema.

[0080] Comparative Example 2

[0081] Randomly select 3 test plots for planting of Millettia pachynema, and the weed control method for the Millettia pachynema breeding field comprises:

[0082] Step one, soil closure: after irrigating the soil of the test plot for 4 days, 960 g / L propisochlor is applied at the recommended dosage (100 mL / mu) according to the instructions of the agent, and the test plot is suitable for sowing on the ground;

[0083] Step two, same as Example 6;

[0084] Step three, same as Example 6;

[0085] Step four, same as Example 6;

[0086] No control measures are taken at other times during the planting period of Millettia pachynema.

[0087] Comparative Example 3

[0088] Randomly select 3 test plots for planting of Millettia pachynema, and the weed control method for the Millettia pachynema breeding field comprises:

[0089] Step one, soil closure: after irrigating the soil of the test plot for 4 days, 480 g / L fluometuron is applied at the recommended dosage (150 mL / mu) according to the instructions of the agent, and the test plot is suitable for sowing on the ground;

[0090] Step two, same as Example 6;

[0091] Step three, same as Example 6;

[0092] Step four, same as Example 6;

[0093] No control measures are taken at other times during the planting period of Millettia pachynema.

[0094] Comparative Example 4

[0095] Randomly select 3 test plots for planting of Millettia pachynema, and the weed control method for the Millettia pachynema breeding field comprises:

[0096] Step one, soil closure: after irrigating the soil of the test plot for 4 days, 960 g / L propisochlor is applied at the recommended dosage (120 mL / mu) according to the instructions of the agent, and the test plot is suitable for sowing on the ground;

[0097] Step two, same as Example 6;

[0098] Step three, same as Example 6;

[0099] Step four, same as example 6;

[0100] No other measures were taken during the planting period of Millettia pachynema.

[0101] Comparative example 5

[0102] Three test plots were randomly selected for Millettia pachynema planting, and the weed control method for Millettia pachynema breeding field included:

[0103] Step one, soil closure: after the test plot soil was irrigated for 4 days, 48% butyl applied at the recommended dosage of the drug use instruction (150 mL / acre), and the test plot surface was suitable for seeding;

[0104] Step two, same as example 6;

[0105] Step three, same as example 6;

[0106] Step four, same as example 6;

[0107] No other measures were taken during the planting period of Millettia pachynema.

[0108] Comparative example 6

[0109] Step one, same as example 6;

[0110] Step two, same as example 6;

[0111] Step three, stem and leaf treatment: the first time to spray the complex pesticide on the stem and leaf at the seedling stage of Millettia pachynema, the complex pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre and 440 g / L fluometuron·Basf 60 mL / acre;

[0112] Step four, same as example 6;

[0113] No other measures were taken during the planting period of Millettia pachynema.

[0114] Comparative example 7

[0115] Step one, same as example 6;

[0116] Step two, same as example 6;

[0117] Step three, stem and leaf treatment: the first time to spray the complex pesticide on the stem and leaf at the five-leaf stage of Millettia pachynema, the complex pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L high-efficiency fluazifop-butyl 60 mL / acre and 440 g / L fluometuron·Basf 60 mL / acre;

[0118] Step four, same as example 6;

[0119] The other time of planting period of the cowslip is not taken any prevention and control measures.

[0120] Comparative Example 8

[0121] Step one, same as Example 6;

[0122] Step two, same as Example 6;

[0123] Step three, stem and leaf treatment: the first time of spraying compound pesticide to the stem and leaf of the cowslip at three leaf stage, the compound pesticide and the dosage are 5% imidazopyrazole acetic acid 180 mL / acre, 60 mL / acre of high-efficiency fluoropyrimidines and 100 mL / acre of precise quinoclamine·fluroxypyr (recommended dosage of the pesticide use instruction), the precise quinoclamine·fluroxypyr includes 6% precise quinoclamine and 16% fluroxypyr;

[0124] Step four, same as Example 6;

[0125] The other time of planting period of the cowslip is not taken any prevention and control measures.

[0126] Comparative Example 9

[0127] Step one, same as Example 6;

[0128] Step two, same as Example 6;

[0129] Step three, stem and leaf treatment: the first time of spraying compound pesticide to the stem and leaf of the cowslip at three leaf stage, the compound pesticide and the dosage are 5% imidazopyrazole acetic acid 180 mL / acre, 60 mL / acre of high-efficiency fluoropyrimidines and 400 mL / acre of 480 g / L triclopyr (recommended dosage of the pesticide use instruction);

[0130] Step four, same as Example 6;

[0131] The other time of planting period of the cowslip is not taken any prevention and control measures.

[0132] Comparative Example 10

[0133] Step one, same as Example 6;

[0134] Step two, same as Example 6;

[0135] Step three, stem and leaf treatment: the first time of spraying compound pesticide to the stem and leaf of the cowslip at three leaf stage, the compound pesticide and the dosage are 5% imidazopyrazole acetic acid 180 mL / acre, 60 mL / acre of high-efficiency fluoropyrimidines and 60 mL / acre of 10% fluroxypyr (recommended dosage of the pesticide use instruction);

[0136] Step four, same as Example 6;

[0137] The other time of planting period of the cowslip is not taken any prevention and control measures.

[0138] Comparative Example 11

[0139] Step one, same as Example 6;

[0140] Step two, same as Example 6;

[0141] Step three, stem and leaf treatment: the first time of spraying compound pesticide on stem and leaf at three-leaf stage of Mimosa pigra, the compound pesticide and the dosage are 5% imazapic 120 mL / acre, 158 g / L flumipropyn 40 mL / acre and 440 g / L fluometuron + benthiocarb 40 mL / acre;

[0142] Step four, same as Example 6;

[0143] No other control measures are taken at other times of Mimosa pigra planting period.

[0144] Comparative Example 12

[0145] Randomly select 3 test plots for Mimosa pigra planting, and the weed control method in Mimosa pigra breeding field includes the first time of spraying compound pesticide on stem and leaf at seedling stage of Mimosa pigra, the compound pesticide and the dosage are 5% imazapic 180 mL / acre, 158 g / L flumipropyn 60 mL / acre and 440 g / L fluometuron + benthiocarb 60 mL / acre;

[0146] Other control measures are the same as Example 1.

[0147] Comparative Example 13

[0148] Randomly select 3 test plots for Mimosa pigra planting, and the weed control method in Mimosa pigra breeding field includes the first time of spraying compound pesticide on stem and leaf at five-leaf stage of Mimosa pigra, the compound pesticide and the dosage are 5% imazapic 180 mL / acre, 158 g / L flumipropyn 60 mL / acre and 440 g / L fluometuron + benthiocarb 60 mL / acre;

[0149] Other control measures are the same as Example 1.

[0150] Comparative Example 14

[0151] Randomly select 3 test plots for Mimosa pigra planting, and the weed control method in Mimosa pigra breeding field includes the first time of spraying compound pesticide on stem and leaf at three-leaf stage of Mimosa pigra, the compound pesticide and the dosage are 5% imazapic 180 mL / acre, 158 g / L flumipropyn 60 mL / acre and quizalofop-P-ethyl + bensulfuron 100 mL / acre (recommended dosage in the pesticide instruction), the quizalofop-P-ethyl + bensulfuron includes 6% quizalofop-P-ethyl and 16% bensulfuron;

[0152] Other control measures are the same as Example 1.

[0153] Comparative Example 15

[0154] Randomly select 3 test plots for planting Millettia pachynema Benth, and spray the compounded pesticide on the stems and leaves for the first time at the three-leaf stage of Millettia pachynema Benth, wherein the compounded pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L flumioxazin 60 mL / acre, and 480 g / L fluroxypyr 400 mL / acre (recommended dosage in the pesticide instruction);

[0155] The other control measures are the same as in Example 1.

[0156] Comparative Example 16

[0157] Randomly select 3 test plots for planting Millettia pachynema Benth, and spray the compounded pesticide on the stems and leaves for the first time at the three-leaf stage of Millettia pachynema Benth, wherein the compounded pesticide and the dosage are 5% imazamox 180 mL / acre, 158 g / L flumioxazin 60 mL / acre, and 480 g / L fluroxypyr 400 mL / acre (recommended dosage in the pesticide instruction);

[0158] The other control measures are the same as in Example 1.

[0159] In Examples 1-6 and Comparative Examples 1-16, wherein Examples 1 and 2 use the screened compounded pesticide in the application to prevent and control weeds in the Millettia pachynema Benth breeding field at the three-leaf stage of Millettia pachynema Benth, the only difference is the dosage of the compounded pesticide; Example 3 further increases the soil sealing prevention and control measure on the basis of Example 1, and the soil sealing uses the pesticide agent screened in the application; Example 4 further increases the Millettia pachynema Benth seed treatment prevention and control measure on the basis of Example 3, and the Millettia pachynema Benth seed treatment method is mechanical rolling; Example 5 further increases the lentinan solution soaking prevention and control measure on the basis of Example 4; and Example 6 further increases the supplementary pesticide application prevention and control measure on the basis of Example 5.

[0160] Comparative Example 1 is a blank control without any preventive measures; Comparative Example 2 differs from Example 6 only in that it uses metolachlor for soil sealing; Comparative Example 3 differs from Example 6 only in that it uses trifluralin for soil sealing; Comparative Example 4 differs from Example 6 only in that it uses S-metolachlor for soil sealing; Comparative Example 5 differs from Example 6 only in that it uses butralin for soil sealing; Comparative Example 6 differs from Example 6 only in that it uses the screened compound pesticide of the present application for stem-leaf treatment at the seedling stage; Comparative Example 7 differs from Example 6 only in that it uses the screened compound pesticide of the present application for stem-leaf treatment at the five-leaf stage; Comparative Example 8 differs from Example 6 only in that it uses imazosulfuron, flumetralin and quizalofop-P+fluazifop for stem-leaf treatment at the three-leaf stage; Comparative Example 9 differs from Example 6 only in that it uses imazosulfuron, flumetralin and fluroxypyr for stem-leaf treatment at the three-leaf stage; Comparative Example 10 differs from Example 6 only in that it uses imazosulfuron, flumetralin and oxyfluorfen for stem-leaf treatment; Comparative Example 11 differs from Example 6 only in that it uses the screened compound pesticide of the present application for stem-leaf treatment, but the application amount of the screened compound pesticide is reduced.

[0161] Comparative Examples 12-16 are comparative tests for Example 1. Comparative Example 12 differs from Example 1 only in that it uses the screened compound pesticide of the present application for stem-leaf treatment at the seedling stage; Comparative Example 13 differs from Example 1 only in that it uses the screened compound pesticide of the present application for stem-leaf treatment at the five-leaf stage; Comparative Example 14 differs from Example 1 only in that it uses imazosulfuron, flumetralin and quizalofop-P+fluazifop for stem-leaf treatment at the three-leaf stage; Comparative Example 15 differs from Example 1 only in that it uses imazosulfuron, flumetralin and fluroxypyr for stem-leaf treatment at the three-leaf stage; Comparative Example 16 differs from Example 1 only in that it uses imazosulfuron, flumetralin and oxyfluorfen for stem-leaf treatment.

[0162] The weeds in the test field of milkvetch were numbered as follows: 1, Capsella bursa-pastoris; 2, Stellaria media (L.) Cyr.; 3, Chenopodium album L.; 4, Chenopodium glaucum L.; 5, Amaranthus retroflexus L.; 6, Portulaca oleracea L.; 7, Convolvulus arvensis L.; 8, Calystegia hederacea Wall.; 9, Lactuca tatarica (L.) C. A. Mey.; 10, Cirsium setosum; 11, Solanum nigrum L.; 12, Descurainia sophia (L.) Webb ex Prantl; 13, Galinsoga parviflora Cav.; 14, Senecio dubitabilis C. Jeffrey & Y. L. Chen; 15, Poa annua L.; 16, Digitaria sanguinalis (L.) Scop.; 17, Leymus secalinus (Georgi) Tzvelev; 18, Oxalis corniculata L.; 19, Kali collinum (Pall.) Akhani & Roalson; and 20, Hibiscus trionum L.

[0163] The results of the comparison of the soil sealing effect of each of Comparative Examples 6 and Comparative Examples 1 to 5 are shown in Table 1.

[0164] Table 1 Soil sealing effect of different land

[0165]

[0166]

[0167] Note: the symbol √ means that the herbicide has a closing effect on weeds, and 0 means that the herbicide has no closing effect; the closing effect means that the herbicide forms a layer of drug film on the surface of the soil, and when the young buds, seedlings or root systems of weeds contact and absorb the herbicide, they are killed; the no closing effect means that although the herbicide forms a layer of drug film on the surface of the soil, when the young buds, seedlings or root systems of weeds contact and absorb the herbicide, they cannot be killed.

[0168] Further comparison of the safety of Millettia pinnata and the fresh weight of weeds in the field after 60 days of application of Example 6 and Comparative Examples 1-5 was made, and the results are shown in Table 2.

[0169] Table 2 Safety of Millettia pinnata and fresh weight of weeds in the field after 60 days of application of different herbicides

[0170]

[0171] Note: different lowercase letters in the table indicate significant differences, i.e. P < 0.05.

[0172] As can be seen from the data in Table 1, there are a total of 20 kinds of weeds in the Millettia pinnata test field after statistics. After spraying isopropyl amine, trifluralin, fine isopropyl amine, sec-butyl and imidazole ethyl nicotine in Comparative Examples 2-5 and Example 6, the number of weeds closed is 6, 8, 12, 13 and 13 (see Table 1) respectively. The number of weeds closed by the closing field provided by Comparative Example 5 and Example 6 is higher, both of which are 13.

[0173] Further investigate the damage size (the death rate of the seedling of the milkvetch) of the soil closed by using each pesticide to the seedling of the milkvetch, the influence on the plant height of the milkvetch and the fresh weight control effect on the weeds in the field. From the statistical data analysis result of table 2, the damage degree of the seedling of the milkvetch of example 6 is the smallest, the death rate of the seedling of the milkvetch is zero, while the seedling of the milkvetch is damaged by the comparative examples 2-5, and the death rate of the seedling of the comparative example 2 is even more than 80%, therefore, from the safety of the seedling of the milkvetch, the soil closed pesticide provided by the application has good safety to the seedling of the milkvetch; from the influence on the plant height of the milkvetch, the plant height difference of example 6 and the comparative example 1 (the control group) is not significant, thus it can be seen that the soil closed pesticide screened by example 6 has little influence on the plant height of the milkvetch, while the plant height of the comparative examples 2-5 is significantly lower than that of the comparative example 1 (the control group), thus it can be seen that the soil closed pesticide of the comparative examples 2-5 has adverse influence on the plant height of the milkvetch, from the influence on the plant height of the milkvetch, the soil closed pesticide provided by the application has little influence on the plant height of the milkvetch; from the fresh weight control effect on the weeds, the fresh weight control effect of example 6 can reach 94.34%, while the fresh weight control effect of the comparative examples 2-5 is poor, and the fresh weight control effect of the comparative example 3 is only 72.33%, therefore, the fresh weight control effect of the soil closed pesticide provided by the application is ideal. In combination with the number of the closed weeds, the damage degree of the seedling of the milkvetch and the control effect on the weeds, the soil closed pesticide of the milkvetch screened by the application is 5% imazamox, and the 5% imazamox can close more weeds, has the smallest damage degree to the seedling of the milkvetch and has a positive effect on the fresh weight control effect on the weeds in the field of the milkvetch.

[0174] Compare example 6, comparative example 6, comparative example 7 and comparative example 11, example 1, comparative example 12 and comparative example 13, and investigate the weed control effect in different periods and different amounts, and the results are shown in table 3.

[0175] Table 3 weed control effect in different periods and different amounts

[0176]

[0177] Note: ++++ the safety performance is that all (or most) plants are dead; +++ the safety performance is that part of the plants are dead and the growth of the plants is seriously affected; ++ the safety performance is that the plants are weak and yellow and the growth of the plants is inhibited; + the safety performance is that the leaves are normal or the top leaves are slightly yellow and the growth is not affected.

[0178] As can be seen from Table 3, the safety level of the screened compound pesticide and the dosage thereof applied in the seedling stage of the Chinese milk vetch is ++ (the same rule is shown in Comparative Example 6 and Comparative Example 12), and the safety thereof to the Chinese milk vetch is poor, so that the screened compound pesticide and the dosage thereof applied in the seedling stage of the Chinese milk vetch are excluded. The safety thereof to the Chinese milk vetch is improved by one level (the same rule is shown in Comparative Example 7, Comparative Example 11, Comparative Example 13, Example 1 and Example 6) when the screened compound pesticide is applied in the three-leaf stage and the five-leaf stage, and the safety level is +, so that the application of the screened compound pesticide in the seedling stage of the Chinese milk vetch is excluded.

[0179] It can be seen from the further investigation of the plant control effect and the fresh weight control effect statistics data that, compared with Comparative Examples 7 to 11, the application period and the application amount of the screened compound pesticide in Example 6 are the optimal choices, and the plant control effect and the fresh weight control effect after 30 days of application are the largest, which are 85.73% and 91.14%, respectively. Compared with Comparative Example 13, the application period of the screened compound pesticide in Example 1 is also the optimal choice, and the plant control effect and the fresh weight control effect after 30 days of application are the largest, which are 77.60% and 89.51%, respectively. At the same time, compared with the data of Example 1, the increase of the control measures in Example 6 has a positive effect on the weed control in the Chinese milk vetch breeding field.

[0180] As shown in Table 4, the safety of the Chinese milk vetch and the weed control effect in the field are compared between Example 6 and Comparative Examples 8 to 10.

[0181] Table 4 Safety of the Chinese milk vetch and weed control effect in the field of different pesticide compound

[0182]

[0183] As can be seen from the data in Table 4, according to the safety observation after 10 days, 20 days and 60 days of application in Example 6 and Comparative Examples 8 to 10, Comparative Example 9 and Comparative Example 15 both use the compound pesticide of imazamox, high-efficiency fluazifop-butyl and triclopyr, and the safety thereof to the Chinese milk vetch is that all (or most) plants are dead (+++), so that the subsequent plant control effect and the fresh weight control effect are not measured, and the stem and leaf treatment of the compound pesticide provided in Comparative Example 9 and Comparative Example 15 is excluded.

[0184] The safety of the imazamox, haloxyfop-R-methyl and fluroxypyr complex pesticides of Comparative Example 10 and Comparative Example 16 to the growth of Millettia pachycarpa is ++, and the safety of Millettia pachycarpa is still poor, so the complex pesticides are excluded from being used for treating Millettia pachycarpa. The safety of the imazamox, haloxyfop-R-methyl and quizalofop-P-fluroxy pyridate of Comparative Example 8 and Comparative Example 14, and the imazamox, haloxyfop-R-methyl and fluoron and benthiocarb of Example 1 and Example 6 to the growth of Millettia pachycarpa is leaf normal or top leaf slightly yellow, and has no effect on growth (+), which indicates that the safety of the imazamox, haloxyfop-R-methyl and quizalofop-P-fluroxy pyridate of Comparative Example 8 and Comparative Example 14, and the imazamox, haloxyfop-R-methyl and fluoron and benthiocarb of Example 1 and Example 6 to the growth of Millettia pachycarpa is good at the dosage used in the test.

[0185] Further from the data of the plant control effect and fresh weight control effect, it can be seen from the data of Comparative Example 8 and Example 6 that, under the same other control measures, the plant control effect of Example 6 to the field weeds of Millettia pachycarpa is the highest, which is 75.37% and 78.85% at 10 days and 20 days after treatment, respectively. The plant control effect of Comparative Example 8 to the field weeds of Millettia pachycarpa is 62.83% and 73.52% at 10 days and 20 days after treatment, respectively. Compared with the complex pesticide provided by Comparative Example 8, the complex pesticide provided by Example 6 has a more excellent plant control effect to the field weeds of Millettia pachycarpa at 10 days and 20 days after treatment. The fresh weight control effect of the complex pesticide provided by Example 6 and Comparative Example 8 to the field weeds of Millettia pachycarpa is 94.34% and 88.38% at 60 days after treatment, respectively. That is, the complex pesticide provided by Example 6 has a more excellent fresh weight control effect to the field weeds of Millettia pachycarpa at 60 days after treatment. In summary, considering the safety factor of Millettia pachycarpa, the plant control effect and fresh weight control effect, the complex pesticide provided by the application and the dosage thereof at the stem and leaf stage are the optimal choice for the integrated control of field weeds of Millettia pachycarpa.

[0186] It can be seen from the data of Comparative Example 14 and Example 1 that, under the same other control measures, the plant control effect of the complex pesticide provided by Example 1 to the field weeds of Millettia pachycarpa is the highest, which is 59.76%, 75.71% and 82.45% at 10 days, 20 days and 60 days after treatment, respectively. The fresh weight control effect of the complex pesticide provided by Example 1 to the field weeds of Millettia pachycarpa is 92.32% at 60 days after treatment, while the fresh weight control effect of the complex pesticide provided by Comparative Example 14 to the field weeds of Millettia pachycarpa is only 84.73% at 60 days after treatment. At the same time, the data of Example 6 compared with Example 1 also proves that increasing the control measures screened by the application has a positive effect on the control of field weeds of Millettia pachycarpa.

[0187] Comparative Example 14 and Example 1, and the imazamox, haloxyfop-R-methyl and fluoron and benthiocarb of Example 1 and Example 6 to the growth of Millettia pachycarpa is leaf normal or top leaf slightly yellow, and has no effect on growth (+), which indicates that the safety of the imazamox, haloxyfop-R-methyl and quizalofop-P-fluroxy pyridate of Comparative Example 8 and Comparative Example 14, and the imazamox, haloxyfop-R-methyl and fluoron and benthiocarb of Example 1 and Example 6 to the growth of Millettia pachycarpa is good at the dosage used in the test.

[0188] Table 5 Safety of different control measures on Millettia pinnata and effect of weed control in field

[0189]

[0190] Note: Different small letters in the table represent significant difference, i.e. P < 0.05.

[0191] As can be seen from Table 5, the effects of different control measures on weed control in Millettia pinnata propagation field are quite different. In Example 1, only the stem and leaf period compound pesticide provided by the present application and the preferred dosage were used for weed control in Millettia pinnata propagation field at the three-leaf stage, and after 30 days of prevention and control, the plant control effect and fresh weight control effect were 77.6% and 89.51%, respectively. In Example 2, only the stem and leaf period compound pesticide provided by the present application was used at the three-leaf stage, and the dosage of the compound pesticide was not the preferred dosage, and after 30 days of prevention and control, the plant control effect and fresh weight control effect were 71.64% and 85.62%, respectively. It can be seen that, after 30 days of prevention and control, the plant control effect and fresh weight control effect of weeds in Millettia pinnata field can reach more than 70% and 80%, respectively, by using the compound pesticide provided by the present application and the dosage range. After 30 days of prevention and control, the plant control effect and fresh weight control effect can reach 77.6% and 89.51%, respectively, by using the preferred dosage provided by the present application. On the basis of Example 1, the soil sealing weed control measure was added, and after 30 days, the plant control effect and fresh weight control effect were 79.65% and 90.53% (Example 3), respectively. The plant control effect and fresh weight control effect after 30 days of prevention and control were improved compared with Example 1. It can be seen that, during the Millettia pinnata planting period, adding the soil sealing measure before sowing can effectively improve the effect of weed control in Millettia pinnata field.

[0192] The seed treatment processes of Examples 4-6 are all increased on the basis of Example 3, wherein the seed treatment process of Example 4 includes mechanical rolling treatment, the seed treatment processes of Examples 5 and 6 both include mechanical rolling treatment and lentinan soaking treatment, the increased seed treatment processes of Examples 4-6 not only can improve the seed emergence rate, and the seed emergence rate can even reach more than 95%, but also effectively improve the weed control effect in the field of Millettia pachynema, because the high seed emergence rate of Millettia pachynema limits the growth space of weeds, thereby improving the weed control effect. Example 5 increases the lentinan soaking step relative to Example 4, and the plant control effect and fresh weight control effect of Example 5 after 30 days of use are still improved relative to the plant control effect and fresh weight control effect of Example 4 after 30 days of use, because the lentinan solution makes the germinated seedlings have good drug resistance and disease resistance, thereby ensuring the planting effect of Millettia pachynema; Example 6 further increases the step of supplementary application, and different proportions of pesticides are supplemented according to the field situation of Millettia pachynema, so that the plant control effect and fresh weight control effect of Example 6 after 30 days of use are further improved relative to the plant control effect and fresh weight control effect of Example 5 after 30 days of use, wherein the fresh weight control effect reaches 91.14%, which can effectively control the weeds in the Millettia pachynema breeding field.

[0193] The number of devices and the scale of the process described herein are used to simplify the description of the present application. The application, modification and change of the Millettia pachynema breeding field weed control method of the present application are obvious to those skilled in the art.

[0194] Although the embodiments of the present application have been disclosed as above, it is not limited to the application and implementation listed in the specification and examples, and can be fully applied to various fields suitable for the present application, and additional modifications can be easily realized by those skilled in the art, therefore the present application is not limited to specific details and specific examples shown and described herein, without departing from the general concept defined by the claims and equivalent scope.

Claims

1. A method for weed control in seedbeds of *Gnaphalium affine*, characterized in that: include: The step of spraying the stems and leaves of *Gynostemma pentaphyllum* with compound pesticides for the first time at the three-leaf stage is as follows: 5% imidacloprid 150-210 mL / mu, 158 g / L high-efficiency flupyridine haloxyfop-methyl 50-70 mL / mu, and 440 g / L flufenoxuron·benzanol 50-70 mL / mu. The steps for soil sealing before sowing are as follows: irrigate the soil before sowing the seeds of *Gnaphalium affine*, and apply 100-140 mL / mu of 5% imidazoline after irrigation. Sowing can be carried out after the soil surface is suitable for sowing. The process involves irrigating 20-25 days after the initial application of the compound pesticide, followed by supplemental application 15-20 days later with one or more of the following: 5% imidacloprid, 158 g / L high-efficiency flupyradifurone, and 440 g / L flufenoxuron·benzon. When the average height of the *Cypripedium* var. *mongolicum* is less than 2.5 cm: If the weed control efficacy is <85%, supplement with 100-140 mL / mu of 5% imidacloprid, 10-30 mL / mu of 158 g / L high-efficiency flupyridine, and 10-30 mL / mu of 440 g / L flufenoxuron·benzon. If the weed control efficacy is less than 88% and the weed control rate is 85%, then supplement with 100-140 mL / mu of 5% imidacloprid and 10-30 mL / mu of 158 g / L high-efficiency flupyradifurone. If the weed control efficacy is ≥88%, then apply 100-140 mL / mu of 5% imidazoline. When 2.5cm ≤ average height of *Cypripedium spp.* < 3.0cm: If the weed control efficacy is <85%, supplement with 5% imidacloprid 50-70 mL / mu, 158 g / L high-efficiency flupyridine 10-30 mL / mu and 440 g / L flufenoxuron·benzon 10-30 mL / mu. If the weed control efficacy is less than 88% and the weed control rate is 85%, then supplement with 5% imidazoline 50-70 mL / mu and 158 g / L high-efficiency fluoride 10-30 mL / mu. If the weed control efficacy is ≥88%, then apply 5% imidazoline 50~70mL / mu as a supplement; When the average height of the *Cypripedium* var. *mongolicum* is ≥3.0cm: If the weed control efficacy is <85%, supplement with 20-40 mL / mu of 5% imidacloprid, 10-30 mL / mu of 158 g / L high-efficiency flupyridine, and 10-30 mL / mu of 440 g / L flufenoxuron·benzon. If the weed control efficacy is less than 88% and the weed control rate is 85%, then supplement with 20-40 mL / mu of 5% imidacloprid and 10-30 mL / mu of 158 g / L high-efficiency flupyridine. If the weed control efficacy is ≥88%, then apply 20-40 mL / mu of 5% imidazoline.

2. The method for weed control in *Gynostemma pentaphyllum* seedbeds as described in claim 1, characterized in that, The compound pesticides and their dosages are 180 mL / mu of 5% imidacloprid, 60 mL / mu of 158 g / L high-efficiency flupyridine, and 60 mL / mu of 440 g / L flufenoxuron·benzanol.

3. The method for weed control in *Gynostemma pentaphyllum* seedbeds as described in claim 2, characterized in that, It also includes the processing steps for the seeds of *Gnaphalium affine*, specifically: crushing the seeds of *Gnaphalium affine* evenly 2 to 4 times.

4. The method for weed control in *Gynostemma pentaphyllum* seedbeds as described in claim 3, characterized in that, It also includes the step of soaking the crushed Cyperus rotundus seeds in a 0.01~0.2 mg / mL lentinan solution for 10~30 min.

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