Preparation method of purine nucleoside reagent and purine nucleoside reagent

Abstract

The invention discloses a preparation method of a purine nucleoside reagent, which comprises the following steps: firstly, screening and purifying a limnoperna fortunei soak solution, a mantle cavity solution, an in-situ biological membrane metabolite and the like to obtain a purified solution, and identifying a purine nucleoside component in the purified solution; according to the purine nucleoside component, the purine nucleoside reagent which is strong in target specificity and attracts the larvae of the macrobrachium fortunei can be prepared, the purine nucleoside reagent can be used as bait to enrich the larvae of the macrobrachium fortunei, and then the enriched larvae of the macrobrachium fortunei are removed, so that the number of the larvae of the macrobrachium fortunei entering a pipe duct is reduced, and the yield of the larvae of The influence on the wall surface of the pipe channel caused by the fouling of the macrobrachium coerulea of the water conveying system is further reduced, the roughness of the water conveying pipe channel can be reduced by reducing the biological fouling of the macrobrachium coerulea, and the water conveying energy consumption is saved.

Description

Technical Field

[0001] The invention relates to the technical field of preventing and controlling marsh clams, and in particular to a preparation method of a purine nucleoside reagent and the purine nucleoside reagent. Background Art

[0002] Our benthic mussels, swamp clams, are widely distributed in freshwater river and lake environments in Asia and South America. The life cycle of swamp clams belongs to metamorphosis, and mainly goes through three stages: shellless larvae, juvenile shellfish, and mature fixed stage. Among them, the larval stage individuals are only 20-50μm, and each female swamp clam can release 500,000-1 million swimming larvae each time. A large number of swimming swamp clam larvae are scattered in the water environment. In order to reproduce in groups, a large number of swamp clam individuals need to be gathered. The larvae will be attracted by the population pheromones released by adult shellfish, and they will swim to pursue the pheromones and attach themselves to the vicinity of adult shellfish. When the water diversion project draws raw water from the river and lake environment, the swamp clam larvae are sucked in and accumulate in large quantities on the concrete surface of the inner wall of the pipe and the open channel over time, forming a fixed swamp clam fouling. For many years, no effective, low-cost and easy-to-implement prevention and control measures have been found. Summary of the invention

[0003] In view of the deficiencies in the prior art, the present invention provides a method for preparing a purine nucleoside reagent, a purine nucleoside reagent, and a method for enriching Marsh clam larvae.

[0004] In a first aspect, a method for preparing a purine nucleoside reagent is provided, comprising the following steps:

[0005] The extract is screened and purified to obtain a purified solution, wherein the extract includes one or more of a swamp clam soaking solution, a mantle cavity fluid, and in situ biofilm metabolites; the purine nucleoside components in the purified solution are identified; and the purine nucleoside reagent is prepared according to the purine nucleoside components, wherein the purine nucleoside reagent is used to enrich swamp clam larvae.

[0006] Based on the above embodiments, it is proposed to identify purine nucleoside components that can be used to attract marsh clam larvae through the soaking liquid of marsh clams, the mantle cavity fluid, and the in situ biofilm metabolites, so as to facilitate the configuration of a purine nucleoside reagent with strong target specificity for attracting marsh clam larvae based on the purine nucleoside component. The purine nucleoside reagent can be used as bait to enrich the marsh clam larvae, and then the enriched marsh clam larvae can be removed, thereby reducing the number of marsh clam larvae entering the pipeline, further reducing the impact of marsh clam fouling on the pipeline wall of the water supply system, and can reduce the roughness of the water supply pipeline by reducing the biofouling of marsh clams, thereby saving water supply energy consumption.

[0007] In combination with the first aspect, in some embodiments of the first aspect, the screening uses an ultrafiltration centrifuge tube.

[0008] In combination with the first aspect, in some embodiments of the first aspect, the purification adopts one or more of cross-linked dextran-based resin gel column and chromatography column purification.

[0009] In combination with the first aspect, in some embodiments of the first aspect, the identifying of the purine nucleoside components in the purified solution includes: using nuclear magnetic resonance spectroscopy to identify the purine nucleoside components in the purified solution.

[0010] In a second aspect, a purine nucleoside reagent is provided, wherein the purine nucleoside reagent is prepared according to the preparation method described in any one of the first aspects.

[0011] In a third aspect, a method for enriching larvae of a swamp clam with purine nucleosides is provided, comprising: slowly releasing purine nucleosides through a releasing device, wherein the releasing device is loaded with the purine nucleoside reagent described in any one of the second aspects.

[0012] Based on the above embodiments, the release device can slowly release purine nucleosides into the water at a certain rate to attract the larvae of the marsh clam, thereby facilitating the removal of the enriched marsh clam larvae, thereby reducing the number of marsh clam larvae entering the pipeline, further reducing the impact of the marsh clam fouling in the water supply system on the pipeline wall, and can reduce the roughness of the water supply pipeline by reducing the biofouling of the marsh clam, thereby saving water supply energy consumption.

[0013] In combination with the third aspect, in some embodiments of the third aspect, the release device includes: a sustained-release osmotic rod and a shell, wherein the purine nucleoside reagent is loaded on the sustained-release osmotic rod.

[0014] Based on the above embodiments, the release device can serve as a carrier of the purine nucleoside reagent in any of the above embodiments and slowly release the purine nucleoside, which is beneficial to enrich and remove the larvae of the marsh clam, thereby reducing the biomass of the marsh clam larvae invading the water pipeline with the raw water.

[0015] In combination with the third aspect, in some embodiments of the third aspect, a cylindrical filter layer is provided in the housing, a cavity is formed inside the filter layer, and the slow-release permeable rod is placed in the cavity. Based on the above embodiments, the influence of other substances in the water on the slow-release permeable rod is reduced by multi-layer filtration.

[0016] In combination with the third aspect, in some embodiments of the third aspect, the filter layer may include one or more layers of fine ceramsite, medium ceramsite, and coarse ceramsite.

[0017] In combination with the third aspect, in some embodiments of the third aspect, the water delivery structure is provided with a bracket, the bracket is provided with a convex structural member, the release device is provided with a mounting frame, and the mounting frame is provided with a groove matching the convex structural member.

[0018] Based on the above embodiment, the release device is installed on the bracket at the water level inside the water conveyance structure by clamping the groove on the convex structure, which is convenient for disassembly and replacement of the slow-release permeation rod.

[0019] In combination with the third aspect, in some embodiments of the third aspect, the sustained-release osmotic rod is prepared by the following process: adding powdered zeolite to water in proportion and stirring thoroughly to obtain a zeolite suspension and adding the purine nucleoside reagent; continuing stirring until the powder is dissolved and adsorbed to obtain a zeolite suspension containing the purine nucleoside reagent; mixing the zeolite suspension with an aqueous sodium alginate solution to obtain a mixture and pouring it into a mold, wherein the size of the mold meets the requirements of the cavity; freezing the mixture to fix it into a solid; pouring the solid into an aqueous magnesium chloride solution and letting it stand to allow a cross-linking reaction to occur; filtering, washing, and drying to obtain the sustained-release osmotic rod. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of a method for preparing a purine nucleoside reagent for a method for preparing a sediment soil conditioner provided in this application;

[0021] Figure 2 A purine nucleoside system device for identifying and removing larvae of a marsh clam provided in this application;

[0022] Figure 3 An example diagram of a sustained-release device provided in this application;

[0023] Figure 4 An example diagram of a mounting frame provided for this application;

[0024] Figure 5 This is an example diagram of the installation effect provided for this application.

[0025] In the figure, 1, solvent bottle; 2, pump; 3, injector; 4, gas chromatograph-mass spectrometer; 5, detector; 6, recorder;

[0026] 10. slow-release device; 100. shell; 110. cavity; 120. filter layer; 121. fine ceramsite; 122. medium ceramsite; 123. coarse ceramsite; 130. mounting frame; 131. groove; 20. water conveyance structure; 21. bracket. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The existing methods for killing marsh clam larvae mainly include:

[0029] 1. Chemical killing method: Currently, a variety of chemical agents have been studied for the killing of biological fouling. Due to the unknown potential ecological risks of chemical agents and the high cost of large-scale application, there are few examples of their application in large-scale water diversion projects.

[0030] 2. Orifice turbulence pulsation killing: Using the different pulse waves generated by water flowing through orifices of different specifications under the impetus of the power system that promotes the flow of water, it can effectively and selectively kill harmful microorganisms in the water within a certain length range. Although it can meet the use requirements of the water source, it will consume the kinetic energy of the water. In addition, if the suspended physical particles in the water are large, the installation of the multi-stage orifice plate will be blocked, affecting the safety of water supply, and is not suitable for large-scale water diversion and regulation projects.

[0031] 3. Biological attachment killing method: Based on the enrichment, attachment and growth characteristics of freshwater shellfish larvae, use attachment rows (or fine-diameter monofilament gauze) to enrich the larvae, and then kill the larvae by regularly replacing the attachment rows. However, it takes manpower and material resources to replace the attachment rows in a timely manner. The larval killing pool involving the fine-diameter monofilament gauze needs to set up a corresponding static water sedimentation area, which is not conducive to the promotion of large-scale water diversion and regulation projects (it is easy to cause siltation).

[0032] 4. Biological sedimentation killing method: The larvae of the marsh clam are light in weight and their natural sedimentation rate is very slow. Therefore, the sedimentation tank needs to occupy a large flat space, which does not meet the conditions for engineering construction and has poor promotion applicability.

[0033] 5. Ultrasonic killing of swamp clams: Large-scale water diversion projects have a large amount of water. The contact time between the swamp clam larvae in the water and the ultrasonic generating device is short. Therefore, it is necessary to deploy ultrasonic generating devices over a long distance, which is expensive and has poor promotion.

[0034] The above methods for killing larvae of the marsh clam can be divided into three categories. Chemical killing will change the physical and chemical properties and ecological functional characteristics of the water body, and is not suitable for large-flow raw water diversion processes. Physical killing (including orifice turbulence killing, biological attachment killing, biological sedimentation killing and ultrasonic killing) targets all full-section fluids, with large water blocking areas and low efficiency, and is not suitable for large-scale water diversion projects driven by electrical energy.

[0035] In addition, for chemical killing, studies have shown that in order to effectively kill the clams in the diversion water, it is necessary to maintain a chemical agent (sodium hypochlorite) concentration of at least 2 mg / L. Unlike the water supply network, the introduction of a large amount of chemical agents in large-scale diversion projects will inevitably change the physical and chemical properties of the raw water, thereby affecting the ecological water replenishment function of large-scale diversion projects. For orifice plate turbulent pulsation killing, it mainly uses the pulse turbulence (turbulence) generated by the kinetic energy of the water body when passing through orifices (apertures) of different specifications to kill the larvae of the corresponding body length of the shell vegetable. This method is not suitable for water bodies with high turbidity, and it will also cause a large head loss. The biological attachment and biological sedimentation killing method is mainly based on the active attachment characteristics of the larvae ... In addition, the adsorbent material needs to be disassembled or naturally settled after adsorption to be completely disinfected, and an additional settling area needs to be set up, which increases the difficulty of the project and has poor promotion. For ultrasonic killing, the contact time between the larvae of the marsh clam in the water body and the ultrasonic generator is short. Therefore, large-scale water diversion projects have a large amount of water, so long-distance ultrasonic generators need to be deployed, which is expensive and has poor promotion.

[0036] Aiming at the problem that a large number of marsh clam larvae accumulate on the concrete surface of the inner wall of pipes and open channels to form solid marsh clam fouling, this application will provide a determination method and application device for removing purine nucleosides of marsh clam larvae. This application is particularly suitable for long-distance raw water transmission projects and power cooling water circulation systems.

[0037] A method for preparing a purine nucleoside reagent, comprising:

[0038] S100, screening and purifying the extract to obtain a purified solution, wherein the extract includes one or more of a slough clam soaking solution, a mantle cavity fluid, and an in situ biofilm metabolite;

[0039] S300, identifying purine nucleoside components in the purified solution;

[0040] S400, preparing the purine nucleoside reagent according to the purine nucleoside component, wherein the purine nucleoside reagent is used to enrich the larvae of the giant clam.

[0041] Among them, the above-mentioned purine nucleoside components can attract the larvae of the marsh clam to attach.

[0042] Based on the above embodiments, it is proposed to identify a purine nucleoside component that can be used to attract marsh clam larvae through the soaking liquid of marsh clams, the mantle cavity fluid, and the in situ biofilm metabolites. The component is a pheromone that attracts marsh clam larvae, which is conducive to configuring a purine nucleoside reagent with strong target specificity for attracting marsh clam larvae based on the purine nucleoside component. The purine nucleoside reagent can be used as bait to enrich the marsh clam larvae, and then the enriched marsh clam larvae can be removed, thereby reducing the number of marsh clam larvae entering the pipeline, further reducing the impact of marsh clam fouling on the pipeline wall of the water supply system, and can reduce the roughness of the water supply pipeline by reducing the biofouling of marsh clams, thereby saving water supply energy consumption.

[0043] In some embodiments, the screening in step S100 may be performed using an ultrafiltration centrifuge tube.

[0044] In some embodiments, the purification in step S100 can be performed by one or more of a cross-linked dextran resin gel column and a chromatographic column, for example, a Sephadex LH20 gel column, a high performance hydraulic chromatography (HPLC) column, etc.

[0045] In some embodiments, the identifying of the purine nucleoside components in the purified solution in step S200 includes: identifying the purine nucleoside components in the purified solution using nuclear magnetic resonance spectroscopy.

[0046] Figure 1 This is an embodiment of the purine nucleoside system device for identifying and removing larvae of the marsh clam provided in this application. Figure 1 As shown, the solution in the solvent bottle 1 is pumped into the injector 3 through the pump 2, the purified liquid and the solution are input into the gas chromatography-mass spectrometer 4 through the injector 3, and the result is introduced into the recorder 6 through the detector 5. Among them, the solution in the solvent bottle 1 can be substances such as the soaking liquid of the swamp clam, the mantle cavity liquid, and the in-situ biofilm metabolites.

[0047] According to the test results, the contents of various purine nucleoside components in the solution are determined. In some embodiments, the purine nucleoside components include adenine, guanine, hypoxanthine and one or more of adenine nucleoside, guanine nucleoside and hypoxanthine nucleoside.

[0048] Exemplarily, the chromatographic conditions can be as follows: using a C18 SB-AQ (250mm×4.6mm, 5μm) chromatographic column, 10mM ammonium acetate as mobile phase A, methanol as mobile phase B, linear gradient elution [0-10min, AB (98:2); 10-30min, AB (98:2)→AB (20:80); 30-40min, AB (20:80)], flow rate 1.0ml / min, column temperature 30°C, detection wavelength 254nm, injection volume 20μL, split ratio 1:1 for MS detection; mass spectrometry conditions can be as follows: electrospray positive ionization, TOF / MS measurement spray voltage 3.5kV, nebulizing nitrogen pressure 275kPa, flow rate 10.0ml / min, temperature 350°C, fragmentation voltage 135V, mass number scanning range 100-1000m / z.

[0049] According to the identification results of purine nucleoside components, a purine nucleoside reagent with corresponding components can be prepared. The reagent can be used to attract the larvae of the marsh clam, thereby enriching the larvae of the marsh clam and removing them.

[0050] In some embodiments, the solution of the purine nucleoside reagent can be pressurized in a dissolved gas tank, and microbubbles containing purine nucleoside components and having a size of 30 μm to 50 μm can be released using a dissolved gas releaser, thereby using the microbubbles as carriers of purine nucleosides to attract a large number of marsh clam larvae distributed in the water. Since the size of the microbubbles is close to that of the larvae, the microbubbles and the marsh clam larvae intercept and collide, thereby enriching the marsh clam larvae in the floating water.

[0051] Furthermore, fluid simulation software such as Fluent can be used to analyze the correlation characteristics of water flow velocity, microbubble floating and migration, so as to confirm the enrichment position of the larvae on the water surface and carry out targeted removal. Specifically, a rotary screen machine can be used to remove the larvae on the water surface by adhering to them.

[0052] The present application also provides a purine nucleoside reagent, which is prepared by any of the above-mentioned methods for preparing purine nucleoside reagents.

[0053] The present application also provides a method for enriching larvae of swamp clams, comprising:

[0054] Purine nucleosides are slowly released through the release device 10, wherein the release device 10 is loaded with a purine nucleoside reagent, and the purine nucleoside reagent is used to enrich the larvae of the marsh clam.

[0055] Based on the above embodiments, the release device 10 can serve as a carrier of the purine nucleoside reagent in any of the above embodiments and slowly release the purine nucleoside, which is beneficial to enrich and remove the larvae of the marsh clam, thereby reducing the biomass of the marsh clam larvae invading the water pipeline with the raw water.

[0056] In some embodiments, Figure 3 As shown, the release device 10 may include: a sustained-release osmotic rod and a shell 100, wherein the purine nucleoside reagent is loaded on the sustained-release osmotic rod.

[0057] In some embodiments, a cylindrical filter layer 120 is further disposed in the housing 100 , and a cavity 110 is formed inside the filter layer 120 . The sustained-release osmotic rod can be placed in the cavity 110 .

[0058] Based on the above embodiments, the influence of other substances in water on the slow-release osmotic rod is reduced through multi-layer filtration.

[0059] In some embodiments, the filter layer 120 may include one or more layers of fine ceramsite 121, medium ceramsite 122, and coarse ceramsite 123. For example, Figure 3 As shown, the filter layer is respectively wrapped by fine ceramsite 121 with a particle size of 1-2 mm from the center to the outside, then wrapped by medium ceramsite 122 with a particle size of 2-10 mm, and finally wrapped by coarse ceramsite 123 with a particle size of more than 10 mm.

[0060] In some embodiments, Figure 5 As shown, the water delivery structure 20 is provided with a bracket 21, and the bracket 21 is provided with a convex structure, such as Figure 4 As shown, the release device 10 is provided with a mounting frame 130, and a groove 131 matching the above-mentioned convex structure is provided on the mounting frame 130. Based on the above embodiment, the release device 10 is installed on the bracket 21 at the water level line inside the water conveyance structure 20 by clamping the groove 131 on the convex structure, so as to facilitate the disassembly and replacement of the slow-release permeation rod.

[0061] Exemplarily, the water conveyance structure 20 includes a channel, a water conveyance tunnel, an aqueduct, a culvert, a culvert, and the like.

[0062] In some embodiments, the sustained-release osmotic rod can be prepared by the following process:

[0063] (1) adding powdered zeolite to water in proportion and stirring thoroughly to obtain a zeolite suspension and adding a purine nucleoside reagent;

[0064] (3) continuing stirring until the powder is dissolved and adsorbed to obtain a zeolite suspension containing the purine nucleoside reagent;

[0065] (4) mixing the zeolite suspension containing purine nucleoside with the sodium alginate aqueous solution and pouring the mixture into a mold, wherein the size of the mold meets the requirements of the sustained-release material cavity;

[0066] (5) freezing the mixture, for example, at a temperature below -10°C, thereby freezing it into a solid state;

[0067] (6) pouring the solid into a magnesium chloride aqueous solution and leaving it to stand at room temperature, for example, for 0.5 h, to allow a cross-linking reaction to occur;

[0068] (7) Filtering, washing and drying the above reaction product to obtain a sustained-release osmotic rod.

[0069] Exemplarily, the purine nucleoside reagent can be adenine, guanine, hypoxanthine and a mixed powder of adenine nucleoside (adenosine), guanine nucleoside (guanosine) and hypoxanthine nucleoside with a purity of ≥98%;

[0070] In some embodiments, the zeolite suspension containing purine nucleoside and the sodium alginate aqueous solution are stirred for no less than 12 hours during the thorough mixing.

[0071] In some embodiments, the step (5) is to freeze the mixture at -20°C for 1 hour.

[0072] In some embodiments, in order to maintain the slow-release concentration, the fixed carrier enriched with the marsh clam larvae can be replaced every two weeks.

[0073] Example 1

[0074] ⑴ 4.00g of powdered zeolite was dissolved in 36ml of ultrapure water to prepare a zeolite suspension, and then 0.268g of guanine powder was dissolved in the zeolite suspension, and 2.00g of sodium alginate was dissolved in 98ml of ultrapure water. The zeolite suspension containing 0.268g of guanine powder was mixed with the above-prepared sodium alginate aqueous solution and stirred for 12h, then poured into a cylindrical mold with a diameter of 0.8cm and a height of 1.1cm and frozen into a solid. Then, it was poured into a magnesium chloride aqueous solution prepared by dissolving 4.00g of anhydrous magnesium chloride in 196ml of ultrapure water and reacted for 0.5h, filtered, washed with deionized water 3 times, and dried at 60°C for 4h to obtain 2mg / cm 3 Guanine sustained-release osmotic rod.

[0075] ⑵Install the guanine sustained-release osmotic rod into the cavity of the sustained-release device.

[0076] ⑶Install the slow-release device on the slow-release bracket.

[0077] (4) After running for about one month, a new slow-release material chamber is replaced, and the replaced slow-release material chamber uses physical means (such as high temperature, ultrasound, ultraviolet light, etc.) to regenerate the penetration rod after enriching the larvae of the marsh clam.

[0078] Example 2

[0079] The difference from the preparation process of Example 1 is that the amount of guanine is 0.134 g, and 2 mg / cm 3 Guanine sustained-release osmotic rod.

[0080] Comparative Example 1

[0081] The difference from the preparation process of Example 1 is that the amount of guanine is 0g, and 0mg / cm 3 Guanine sustained-release osmotic rod.

[0082] Sampling and testing of some sustained-release material cavities within one month. Testing method: scrape the same position and equal area of ​​the external area of ​​the sustained-release material cavity, take samples of the scraping residue, and test a blood cell counting plate and a counting chamber (volume 0.1mm) under a microscope. 3 That is 1x10 -4 The number of larvae of the marsh clam on the mL) sample was measured. The test results are shown in Table 1.

[0083] Table 1 The number of larvae per unit area of ​​the osmotic rods with different sustained-release concentrations of guanine

[0084]

[0085] The test results are shown in Table 1. The higher the guanine concentration, the better the enrichment effect of the penetration rod on the larvae of the marsh clam. 3 The enrichment effect of the guanine slow-release osmotic rod is about 2.23 times higher than that of the slow-release osmotic rod without guanine. It can be seen that the release device can serve as a carrier of the above-mentioned guanine and slowly release the purine nucleoside, which is conducive to enriching and removing the larvae of the marsh clam, thereby reducing the biomass of the marsh clam larvae invading the water pipe with the raw water.

[0086] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0087] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a purine nucleoside reagent, characterized in that: The following steps are involved: Screening and purifying the extract to obtain a purified solution, wherein the extract is one or more of a soaking solution of a clam, a mantle cavity fluid, and an in-situ biofilm metabolite; identifying purine nucleoside components in the purified solution; The purine nucleoside reagent is prepared according to the purine nucleoside component, wherein the purine nucleoside reagent is used to enrich the larvae of the marsh clam.

2. The preparation method according to claim 1, characterized in that: The screening adopts an ultrafiltration centrifuge tube.

3. The preparation method according to claim 1 or 2, characterized in that: The purification adopts one or more of cross-linked dextran resin gel column and chromatographic column purification.

4. The preparation method according to claim 1, characterized in that: The method of identifying the purine nucleoside components in the purified solution comprises: The purine nucleoside components in the purified solution were identified by nuclear magnetic resonance spectroscopy.

5. A purine nucleoside reagent, characterized in that: The purine nucleoside reagent is prepared according to the preparation method according to any one of claims 1-4.

6. A method for enriching larvae of Marsh Clams, characterized in that: include: The purine nucleoside is slowly released by a release device, wherein the release device is loaded with the purine nucleoside reagent according to claim 5.

7. The method according to claim 6, characterized in that The release device comprises: a sustained-release osmotic rod and a shell, wherein the purine nucleoside reagent is loaded on the sustained-release osmotic rod.

8. The method according to claim 7, characterized in that A cylindrical filter layer is arranged in the shell, a cavity is formed inside the filter layer, and the slow-release permeation rod is placed in the cavity.

9. The method according to any one of claims 6 to 8, characterized in that: The water conveyance structure is provided with a bracket, on which a convex structural member is provided. The release device is provided with a mounting frame, and the mounting frame is provided with a groove matching the convex structural member.

10. The method according to claim 8, characterized in that The sustained-release osmotic rod is prepared by the following process: Adding powdered zeolite to water in proportion and stirring thoroughly to obtain a zeolite suspension, and then adding the purine nucleoside reagent; Continue stirring until the powder is dissolved and adsorbed to obtain a zeolite suspension containing the purine nucleoside reagent; Mixing the zeolite suspension with a sodium alginate aqueous solution to obtain a mixture and pouring the mixture into a mold, wherein the size of the mold meets the requirements of the cavity; Freezing the mixture into a solid state; Pour the solid into a magnesium chloride aqueous solution and allow to stand to allow a cross-linking reaction to occur; Filter, wash and dry to obtain the sustained-release osmotic rod.

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