Application of traditional Chinese medicine composition in preparation of medicine for relieving cough and reducing sputum

Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to application of a traditional Chinese medicine composition in preparing a medicine for relieving cough and reducing sputum. The traditional Chinese medicine composition is prepared from 5-30 parts of herba houttuyniae, 5-30 parts of honeysuckle flowers, 5-20 parts of radix paeoniae rubra, 3-15 parts of folium artemisiae argyi and 3-15 parts of herba menthae. The traditional Chinese medicine composition is found to be capable of remarkably prolonging the cough latency period of mouse cough caused by ammonia water spraying, reducing the cough frequency of mice, remarkably improving the phenol red amount in mouse bronchial lavage fluid and increasing the phlegm reducing rate, and the traditional Chinese medicine composition has the effects of relieving cough and reducing phlegm.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to the application of a traditional Chinese medicine composition in preparing a medicine for relieving cough and reducing phlegm. Background Art

[0002] Phlegm is mucus secreted by the respiratory tract. Coughing it out can keep the respiratory tract clean and unobstructed. Excessive phlegm is a common symptom of respiratory diseases. If the phlegm cannot be discharged, not only will the disease be difficult to cure, but it will also easily block the respiratory tract, causing breathing difficulties. In severe cases, it can cause breathing difficulties and endanger life.

[0003] Cough is also a common respiratory symptom, caused by inflammation, foreign bodies, physical or chemical stimulation of the trachea, bronchial mucosa or pleura. Symptoms include first closing of the glottis, contraction of the respiratory muscles, increased intrapulmonary pressure, and then opening of the glottis. The air in the lungs is ejected, usually accompanied by sound. Cough may include wet cough, dry cough, allergic cough, etc.

[0004] There are many causes of cough, mainly including the following aspects: Environmental factors: When the ambient temperature drops, inhaling cold air may cause coughing. At the same time, irritating gases such as car exhaust and sulfur dioxide may also irritate the bronchi and cause coughing. Infection: Coughing is closely related to respiratory tract infections, including infections such as bacteria, viruses, and mycoplasma. These infections can cause acute or chronic bronchitis, leading to the appearance of coughing symptoms. Allergies: Inhaling allergic substances such as pollen, dander, and dust may also cause coughing. In addition, food allergies are also an important cause, especially infants and young children are prone to allergic reactions to foods such as fish, shrimps, crabs, eggs, and milk. Climate change: Changes in temperature, air pressure, and ions in the air may induce coughing. The incidence of coughing is relatively high in cold seasons or when the climate changes in autumn and winter. Mental factors: Emotional excitement, nervousness, resentment, and other mental factors may also induce coughing. Exercise: Some patients may induce coughing after strenuous exercise, which is called exercise-induced cough or exercise cough. Drug factors: Certain drugs such as angiotensin converting inhibitors (such as enalapril, benazepril) and propranolol may cause cough as an adverse reaction.

[0005] At present, commonly used cough suppressants include Chinese medicine and Western medicine. Western medicine has the characteristics of clear targets and quick effects. Western medicine usually contains fewer ingredients, and its main functions are expectoration, anti-allergy and bronchial dilation, which can quickly relieve cough symptoms. However, Western medicine also has some disadvantages, such as more side effects, and some drugs may cause adverse reactions such as drowsiness, headache, nausea, etc. In addition, although Western medicine can quickly relieve symptoms, it cannot fundamentally solve the cause of cough, and repeated coughing may occur. Chinese medicine has relatively fewer side effects and is safer to take for a long time. However, most of the cough suppressants on the market are very complex in composition and the effects are not clear enough. In recent years, with the deepening of medical research, the treatment of cough and sputum has been evolving. The field of traditional Chinese medicine should also actively intervene in the work of cough and expectoration, and develop prescriptions for symptoms. Summary of the invention

[0006] In view of this, the technical problem to be solved by the present invention is to provide an application of a Chinese medicine composition in the preparation of a medicament for relieving cough and reducing phlegm.

[0007] The present invention provides the use of a Chinese medicine composition in preparing a medicament for relieving cough and / or resolving phlegm;

[0008] The Chinese medicine composition comprises the following raw materials in parts by weight: 5-30 parts of houttuynia cordata, 5-30 parts of honeysuckle, 5-20 parts of red peony root, 3-15 parts of wormwood leaves, and 3-15 parts of mint.

[0009] In the present invention, the cough relief includes: reducing the number of coughs and / or prolonging the cough latency period.

[0010] The incubation period of a cough refers to the period of time from when the body is exposed to the triggering factor that causes a cough to when the cough symptom appears. The length of this period of time reflects the effectiveness of the drug's effect on the body. A prolonged incubation period of a cough indicates that the administered drug is effective. The results of the embodiments of the present invention show that administering the Chinese medicine composition can effectively reduce the number of coughs and prolong the duration of coughing in mice after receiving stimulation.

[0011] In the present invention, the expectoration comprises: increasing the amount of sputum secretion.

[0012] Sputum is mainly composed of glandular secretions, necrotic tissue, bacteria and foreign matter in the airway. When the respiratory tract is irritated or infected, the amount of sputum secretion will increase. Expectorant means helping the body to expel sputum accumulated in the respiratory tract through a series of methods, thereby relieving symptoms such as coughing and shortness of breath. The present invention shows through the phenol red test that the administration of the Chinese medicine composition as described above is conducive to increasing the secretion of sputum and playing the role of expectorant.

[0013] There are many factors that lead to coughing. In the present invention, the cough is caused by irritating gases. In a specific embodiment, the irritating gas is ammonia gas and / or ammonia water spray.

[0014] In an embodiment of the present invention, the mass fractions of each component in the traditional Chinese medicine composition are: 5-25 parts of houttuynia cordata, 5-25 parts of honeysuckle, 5-15 parts of red peony root, 3-10 parts of wormwood, and 3-10 parts of mint. Furthermore, the mass fractions of each component in the traditional Chinese medicine composition are: 15-25 parts of houttuynia cordata, 15-25 parts of honeysuckle, 10-15 parts of red peony root, 5-10 parts of wormwood, and 3-5 parts of mint.

[0015] Optionally, the mass proportions of each component in the traditional Chinese medicine composition are: 20 parts of Houttuynia cordata, 20 parts of Honeysuckle, 12 parts of Red Peony Root, 8 parts of Artemisia argyi, and 4 parts of mint.

[0016] Optionally, the mass proportions of each component in the traditional Chinese medicine composition are: 15 parts of Houttuynia cordata, 15 parts of Honeysuckle, 12 parts of Red Peony Root, 7 parts of Artemisia argyi, and 7 parts of mint.

[0017] Optionally, the mass proportions of each component in the traditional Chinese medicine composition are: 10 parts of Houttuynia cordata, 10 parts of Honeysuckle, 8 parts of Red Peony Root, 4 parts of Artemisia argyi, and 4 parts of Mint.

[0018] The composition of the present invention can be directly ground into powder, or it can be an extract obtained by conventional means in the art. The composition can be directly ground into powder by conventional methods, or it can be an extract obtained by conventional means, so as to be made into different dosage forms, obtain better therapeutic effects and be more conducive to the preparation of modern pharmaceutical dosage forms.

[0019] In an embodiment of the present invention, the preparation method of the Chinese medicine composition comprises:

[0020] Step A: weighing Chinese medicinal materials according to the weight ratio of the raw materials;

[0021] Step B: extracting honeysuckle with 6-12 times the amount of 50%-80% ethanol, filtering the extract, recovering the ethanol, drying and crushing to obtain a dry paste powder;

[0022] Step C: add 3-12 times of water to Houttuynia cordata, Artemisia argyi and mint to extract volatile oil, add coarse powder of red peony root to the residue, add 6-12 times of water to boil, filter the extract to obtain a clear paste, add ethanol with a concentration of more than 90% to 60% to 80% alcohol, precipitate with alcohol, let stand, recover ethanol from the supernatant to obtain a thick paste, dry and crush to obtain dry paste powder;

[0023] Step D: Mix the volatile oil and the two dry paste powders.

[0024] Furthermore, the method for preparing the Chinese medicine composition comprises the following steps:

[0025] Step A: Add 12 times the amount of 50% ethanol to honeysuckle, reflux extract twice, each time for 0.5 hours, filter, combine the filtrate, recover the ethanol under reduced pressure and concentrate to a thick paste with a relative density of 1.20 to 1.25, dry under reduced pressure, and crush to obtain alcohol-extracted dry extract powder;

[0026] Step B: add 8 times the amount of water to Houttuynia cordata, Artemisia argyi and mint, extract volatile oil by steam distillation for 5 hours, filter the extract and set aside;

[0027] Step C: grinding and inclusion of the obtained volatile oil with 10 times the amount of β-cyclodextrin and 20 times the amount of water for 20 minutes, drying and crushing the inclusion compound to obtain a volatile oil inclusion compound;

[0028] Step D: after extracting the volatile oil, the residue is combined with red peony root, 10 times the amount of water is added, extraction is performed twice, each time for 1 hour, the extract is combined with the oil extraction liquid, filtered, the filtrate is concentrated under reduced pressure to a clear paste with a relative density of 1.02-1.05, cooled to room temperature, 95% ethanol is added to make the alcohol content reach 70%, and the mixture is allowed to stand for more than 12 hours. The supernatant of the alcohol precipitation is reduced in pressure to recover ethanol and concentrated to a thick paste with a relative density of 1.25-1.30, dried under reduced pressure, and crushed to obtain a water-extracted dry extract powder; the alcohol-extracted dry extract powder, the volatile oil inclusion compound and the water-extracted dry extract powder are mixed.

[0029] The present invention also provides a cough and phlegm-relieving medicine, which comprises pharmaceutically acceptable excipients and the following raw materials in parts by weight: 5-30 parts of houttuynia cordata, 5-30 parts of honeysuckle, 5-20 parts of red peony root, 3-15 parts of wormwood leaf, and 3-15 parts of mint.

[0030] The dosage form of the drug of the present invention includes but is not limited to decoction, granules, capsules, tablets, oral liquids, pills, tinctures, syrups, suppositories, gels, sprays and / or injections.

[0031] In some embodiments provided by the present invention, the capsule is a hard capsule or a soft capsule.

[0032] In some embodiments provided herein, the tablet is an oral tablet or an oral tablet.

[0033] Oral tablets refer to tablets for oral administration. Most of the drugs in such tablets are absorbed through the gastrointestinal tract to exert their effects, and some of the drugs in tablets exert their effects locally in the gastrointestinal tract. In some embodiments provided by the present invention, the oral tablets are ordinary compressed tablets, dispersible tablets, effervescent tablets, chewable tablets, coated tablets or sustained-release tablets.

[0034] The pharmaceutically acceptable excipients include one or a mixture of two or more of fruit powder, edible flavors, sweeteners, acidulants, fillers, lubricants, preservatives, suspending agents, edible pigments, diluents, emulsifiers, disintegrants or plasticizers.

[0035] The medicine described in the present invention also includes other cough and phlegm relieving medicines, and the other cough and phlegm relieving medicines include: at least one of dextromethorphan guaifenesin, ambroxol hydrochloride, fudosteine, bromhexidine hydrochloride, acetylcysteine, benproperine phosphate, carbocysteine, and ammonium chloride.

[0036] In the present invention, in the medicine, the Chinese medicine composition and other therapeutic agents may exist independently of each other or may exist in combination, and the present invention does not limit this.

[0037] Furthermore, the present invention also provides a method for relieving cough and reducing phlegm, which comprises administering the medicine of the present invention.

[0038] The administration methods of the drug include, but are not limited to, oral administration, spray inhalation, rectal administration, nasal administration, buccal administration, vaginal administration, topical administration. Or non-enteral administration, such as subcutaneous, intravenous, intramuscular, intraperitoneal, intrathecal, intraventricular, intrasternal or intracranial injection or infusion; or administration via an explanted reservoir. Preferably, the administration method is oral administration and / or aerosol inhalation.

[0039] In the present invention, when the medicine includes a Chinese medicine composition and other therapeutic agents, two or more medicines can be administered simultaneously or sequentially, and the present invention is not limited to this.

[0040] For example, the Chinese medicine composition in the medicine is administered no longer than 6 hours, preferably no longer than 5 hours, more preferably no longer than 4 hours, even more preferably no longer than 3 hours, more preferably no longer than 2 hours and most preferably no longer than 1 hour before the administration of other therapeutic agents and / or no longer than 6 hours, preferably no longer than 5 hours, more preferably no longer than 4 hours, even more preferably no longer than 3 hours, more preferably no longer than 2 hours and most preferably no longer than 1 hour after the administration of other therapeutic agents.

[0041] In the present invention, when the medicine includes a Chinese medicine composition and other therapeutic agents, the administration frequency of two or more medicines may be the same or different, and the present invention is not limited to this.

[0042] For example, the administration frequency of two or more of the drugs is independently selected from 4 times / day, 3 times / day, 2 times / day or 1 time / day, wherein the administration frequency of the Chinese medicine composition is 4 times / day, 3 times / day, 2 times / day or 1 time / day.

[0043] In the present invention, the medicine includes a Chinese medicine composition, and the dosage is not less than the effective dose. If the medicine includes a Chinese medicine composition and other therapeutic agents, the dosage of the Chinese medicine composition is lower than the effective dose, or not lower than the effective dose; the dosage of other therapeutic agents is lower than the effective dose, or not lower than the effective dose.

[0044] In the present invention, the effective dose includes a "therapeutically effective amount" or a "prophylactically effective amount", which refers to an amount that is sufficient to treat or prevent a patient's disease but low enough to avoid serious side effects (at a reasonable benefit / risk ratio) within the scope of reasonable medical judgment. The preventive or therapeutic effective amount of a drug will vary depending on the specific drug selected (e.g., considering the efficacy, effectiveness and half-life of the drug), the selected route of administration, the disease to be prevented or treated, the severity of the disease to be prevented or treated, the age, size, weight and physical illness of the patient to be prevented or treated, the medical history of the patient to be prevented or treated, the duration of prevention or treatment, the nature of concurrent therapy, the desired preventive or therapeutic effect, and other factors, but can still be routinely determined by those skilled in the art.

[0045] In the present invention, the prevention and treatment includes prevention and / or treatment, which is a preventive and therapeutic measure for cough and sputum. This includes a method for preventing the occurrence of cough, the purpose of which is to prevent, slow down (reduce) undesirable physiological changes or lesions in the treatment subject, and the beneficial or desired clinical results include but are not limited to avoiding the occurrence of cough and sputum, alleviating the symptoms of cough and sputum, reducing the degree of cough and sputum, stabilizing the state of cough and sputum (i.e., not worsening), delaying or slowing the progression of cough, improving or relieving the state of cough and sputum, and alleviating (whether partially or completely), whether detectable or undetectable. The subject in need of treatment includes a subject who has a disease or disease, a subject who is susceptible to a disease or disease, or a subject who intends to prevent a disease or disease.

[0046] In the present invention, the object of prevention and treatment is a human or a mammal, and the mammal includes bovines, equines, ovines, swine, canines, felines, rodents, and primates. The rodent is a mouse and / or a rat.

[0047] The Chinese medicine composition of the present invention comprises 5-30 parts of houttuynia cordata, 5-30 parts of honeysuckle, 5-20 parts of red peony root, 3-15 parts of wormwood leaf and 3-15 parts of mint. The present invention finds that the composition can significantly prolong the cough latency of mice caused by ammonia spray, reduce the number of coughs in mice, significantly increase the amount of phenol red in the bronchial lavage fluid of mice, and increase the expectoration rate, indicating that the Chinese medicine composition of the present invention has the effect of relieving cough and reducing phlegm. DETAILED DESCRIPTION

[0048] The present invention provides the application of Chinese medicine composition in the preparation of medicaments for relieving cough and reducing phlegm, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described by preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the method and application of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0049] The test materials used in the present invention are all common commercial products and can be purchased in the market.

[0050] The following experiments, if no specific conditions are specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The APIs or excipients, reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially. Unless otherwise specified, all percentages, ratios, proportions or parts are by weight.

[0051] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The present invention is further described below in conjunction with the embodiments:

[0052] Example 1 Preparation of Chinese medicine composition

[0053] Ratio of raw materials: Houttuynia cordata: 750g, honeysuckle 750g, red peony root 450g, wormwood leaf 300g, mint 150g.

[0054] Step A: adding 12 times the amount of 50% ethanol to honeysuckle, reflux extraction twice, each time for 0.5 hour, filtering, combining the filtrate, reducing pressure (-0.07MPa to -0.09MPa, 65°C to 75°C) to recover ethanol and concentrate to a thick paste with a relative density of 1.20 to 1.25 (65°C), reducing pressure drying (-0.07MPa to -0.09MPa, 60°C to 65°C), and crushing to obtain alcohol-extracted dry extract powder;

[0055] Step B: add 8 times the amount of water to Houttuynia cordata, Artemisia argyi and mint, and extract volatile oil by steam distillation for 5 hours, filter the extract, and store it in another container for standby use;

[0056] Step C: grinding and inclusion of the obtained volatile oil with 10 times the amount of β-cyclodextrin and 20 times the amount of water for 20 minutes, drying the inclusion compound (55° C.), and crushing to obtain a volatile oil inclusion compound;

[0057] Step D: after extracting the volatile oil, the residue is combined with red peony root, 10 times the amount of water is added, and extraction is performed twice, each time for 1 hour. The extract is combined with the oil extraction liquid, filtered, and the filtrate is concentrated under reduced pressure (-0.07MPa to -0.09MPa, 75°C to 85°C) to a clear paste with a relative density of 1.02 to 1.05 (70°C), cooled to room temperature, and 95% ethanol is added to make the alcohol content reach 70%, and the mixture is allowed to stand for more than 12 hours. The supernatant of the alcohol precipitation is decompressed (-0.07MPa to -0.09MPa, 65°C to 75°C) to recover ethanol and concentrate to a thick paste with a relative density of 1.25 to 1.30 (65°C), and an appropriate amount of dextrin is added to reduce the pressure (-0.07MPa to -0.09MPa, 75°C) to dry, and pulverize to obtain a water-extracted dry extract powder.

[0058] The above three intermediates (dry extract powder of alcohol extraction part, volatile oil inclusion compound, dry extract powder of water extraction part) are mixed with appropriate amount of dextrin and stevioside, and dry granulated to prepare 1000g granules.

[0059] Example 2 Effect of Chinese herbal medicine composition on cough induced by ammonia spray in mice

[0060] Experimental Materials

[0061] Animals: 70 ICR mice, SPF grade, weighing 18-22 g, half male and half female.

[0062] Drug: Dextromethorphan guaifenesin syrup, Shanghai Johnson & Johnson Pharmaceutical Co., Ltd., batch number: 221206234; specification: 150mL / bottle.

[0063] Reagent: Ammonia water, Nanjing Chemical Reagent Co., Ltd., batch number: 181022827K, specification: 500 mL / bottle.

[0064] Instruments: BS224S electronic analytical balance, Beijing Sartorius Instrument System Co., Ltd.; ACX-SC-DA animal weighing scale, Shanghai Xiangchuan Electronic Scale Co., Ltd.; YLS-8A multifunctional cough and wheeze inducing instrument, Shanghai Xinruan Information Technology Co., Ltd.

[0065] Experimental methods

[0066] 70 ICR mice, half male and half female, were selected for pre-selection. The mice were placed in the YLS-8A multifunctional cough and wheeze induction instrument. About 20 mL of ammonia water was added to the atomization cup. The spray was stopped after 5 seconds. The time required to induce coughing in the mice (coughing latency) and the number of coughs within 1 minute were observed and recorded. If the coughing latency was less than 1 minute and there were more than 3 typical coughs within 1 minute, the animals were "coughing" sensitive animals. Otherwise, those without coughs were eliminated.

[0067] Every other day, qualified mice were randomly divided into 5 groups, namely the model control group (pure water), the positive control group (dextromethorphan guaifenesin syrup, 12.3mL / kg), and the low, medium and high dose groups of the Chinese medicine composition (7.38, 14.76, 29.52g crude drug / kg), with 10 mice in each group, half male and half female. Each group of mice was gavaged, and the model control group was gavaged with an equal volume of pure water once a day for 5 consecutive days. One hour after the last administration, the mice were placed in the YLS-8A multifunctional cough and wheezing instrument, and about 20mL of ammonia water was added to the nebulizer cup. The spray was stopped for 5s, and the cough latency and the number of coughs in 3min of the mice were observed and recorded. The cough standard is: abdominal muscle contraction, open mouth at the same time, and sometimes there may be coughing.

[0068] The results showed that compared with the model control group, the low, medium and high dose groups of the Chinese medicine composition and the positive control drug dextromethorphan guaifenesin syrup group could significantly prolong the cough latency period of mice induced by ammonia spray and reduce the number of coughs in mice (P<0.05, P<0.01). The results are shown in Table 1.

[0069] Table 1 Effect of ammonia spray on coughing in mice ( n=10)

[0070]

[0071] Note: Compared with the model control group, *P<0.05, **P<0.01.

[0072] Experimental Example 3 Effect of Chinese herbal medicine composition on phenol red excretion in mice

[0073] Experimental Materials

[0074] Animals: 50 ICR mice, SPF grade, weighing 18-22 g, half male and half female.

[0075] Drug: Ambroxol hydrochloride oral solution, Beijing Hanmei Pharmaceutical Co., Ltd., batch number: 22110002, specification: 120mL / bottle.

[0076] Reagents: phenol red, Aladdin Company, batch number: 130370, specification: 100 mg / tube; sodium bicarbonate, Aladdin Company, batch number: 20220111, specification: 500 g / bottle.

[0077] Instruments: BS224S electronic analytical balance, Beijing Sartorius Instrument System Co., Ltd.; ACX-SC-DA animal weight scale, Shanghai Xiangchuan Electronic Scale Co., Ltd.; Flexstation3 multifunctional microplate reader, MD Company, USA.

[0078] Experimental methods

[0079] Fifty ICR mice weighing 18-22g were selected, half of them were male and half were female, and they were randomly divided into 5 groups according to their body weight, namely, blank control group (pure water), positive control group (ambroxol hydrochloride oral solution, 6.15mL / kg), low-, medium- and high-dose groups of Chinese medicine composition (7.38, 14.76, 29.52g crude drug / kg), 10 mice in each group, half of them were male and half were female. Each group was given the corresponding drug by gavage, and the blank control group was given an equal volume of pure water once a day for 7 consecutive days. Fasting but not water was allowed for 16h before the experiment. One hour after the last drug, 5% phenol red solution (w / v, saline, 10mL / kg) was given by gavage once. After the administration of phenol red, the mice were killed at intervals of 30min, and the trachea between the thyroid cartilage and the main bronchus was taken, and about 1mL of 5% NaHCO3 saline solution was added. After ultrasonication for 15min, the absorbance OD value was measured at 546nm on a Flexstation 3 multifunctional microplate reader. At the same time, 5% NaHCO3 physiological saline solution was used to obtain NaHCO3 physiological saline solutions containing 10, 5, 2.5, 1, 0.5, 0.25, and 0.1 μg / mL of phenol red by multiple dilution method, and samples were taken and their absorbance OD values ​​at 546 nm were measured to draw a phenol red standard curve, and the phenol red amount and sputum reduction rate were calculated according to the standard curve regression equation. Sputum reduction rate (%) = phenol red content in the tracheal lavage fluid of the blank control group / phenol red content in the tracheal lavage fluid of the drug administration group × 100%.

[0080] The results showed that compared with the blank control group, the low, medium and high dose groups of the Chinese medicine composition and the positive control drug ambroxol hydrochloride oral solution group could significantly increase the amount of phenol red in the bronchial lavage fluid of mice (P<0.01) and increase the expectoration rate. The results are shown in Table 2.

[0081] Table 2 Effects of phenol red excretion on mouse trachea segment ( n=10)

[0082]

[0083] Note: Compared with the blank control group, *P<0.05, **P<0.01.

[0084] The traditional Chinese medicine composition proposed by the invention can significantly prolong the cough latency period of mice induced by ammonia spray and reduce the number of coughs of mice; it can significantly increase the amount of phenol red in the bronchial lavage fluid of mice and increase the expectoration rate.

[0085] The above are only preferred embodiments of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Use of a Chinese medicine composition in the preparation of a medicament for relieving cough and / or reducing phlegm; The Chinese medicine composition comprises the following raw materials in parts by weight: 5-30 parts of houttuynia cordata, 5-30 parts of honeysuckle, 5-20 parts of red peony root, 3-15 parts of wormwood leaves, and 3-15 parts of mint.

2. The use according to claim 1, characterized in that: The cough relief includes: reducing the number of coughs and / or prolonging the cough latency period.

3. The use according to claim 1, characterized in that: The expectoration includes: increasing the amount of sputum secretion.

4. The use according to any one of claims 1 to 3, characterized in that: The cough is caused by irritating gas.

5. The use according to any one of claims 1 to 4, characterized in that: The irritating gas is ammonia gas and / or ammonia water spray.

6. The use according to any one of claims 1 to 5, characterized in that: The preparation method of the Chinese medicine composition comprises: Step A: weighing Chinese medicinal materials according to the weight ratio of the raw materials; Step B: extracting honeysuckle with 6-12 times the amount of 50%-80% ethanol, filtering the extract, recovering the ethanol, drying and crushing to obtain a dry paste powder; Step C: add 3-12 times of water to Houttuynia cordata, Artemisia argyi and mint to extract volatile oil, add coarse powder of red peony root to the residue, add 6-12 times of water to boil, filter the extract to obtain a clear paste, add ethanol with a concentration of more than 90% to 60% to 80% alcohol, precipitate with alcohol, let stand, recover ethanol from the supernatant to obtain a thick paste, dry and crush to obtain dry paste powder; Step D: Mix the volatile oil and the two dry paste powders.

7. The use according to claim 6, characterized in that: The method for preparing the Chinese medicine composition comprises the following steps: Step A: Add 12 times the amount of 50% ethanol to honeysuckle, reflux extract twice, each time for 0.5 hours, filter, combine the filtrate, recover the ethanol under reduced pressure and concentrate to a thick paste with a relative density of 1.20 to 1.25, dry under reduced pressure, and crush to obtain alcohol-extracted dry extract powder; Step B: add 8 times the amount of water to Houttuynia cordata, Artemisia argyi and mint, extract volatile oil by steam distillation for 5 hours, filter the extract and set aside; Step C: grinding and inclusion of the obtained volatile oil with 10 times the amount of β-cyclodextrin and 20 times the amount of water for 20 minutes, drying and crushing the inclusion compound to obtain a volatile oil inclusion compound; Step D: after extracting the volatile oil, the residue is combined with red peony root, 10 times the amount of water is added, and extraction is performed twice, each time for 1 hour, the extract is combined with the oil extraction liquid, filtered, and the filtrate is concentrated under reduced pressure to a clear paste with a relative density of 1.02-1.05, cooled to room temperature, 95% ethanol is added to make the alcohol content reach 70%, and the mixture is allowed to stand for more than 12 hours, and the ethanol is recovered from the supernatant of the alcohol precipitation under reduced pressure and concentrated to a thick paste with a relative density of 1.25-1.30, and the mixture is dried under reduced pressure and crushed to obtain a water-extracted dry extract powder; and the alcohol-extracted dry extract powder, the volatile oil inclusion compound and the water-extracted dry extract powder are mixed.

8. A cough and phlegm-relieving drug, comprising pharmaceutically acceptable excipients and the following raw materials in parts by weight: 5-30 parts of Houttuynia cordata, 5-30 parts of Honeysuckle, 5-20 parts of Red Peony Root, 3-15 parts of Artemisia argyi, and 3-15 parts of mint.

9. The drug according to claim 8, characterized in that Other cough and phlegm-relieving drugs are also included, and the other cough and phlegm-relieving drugs include: at least one of dextromethorphan guaifenesin, ambroxol hydrochloride, fudosteine, bromhexidine hydrochloride, acetylcysteine, benproperine phosphate, carbocysteine, and ammonium chloride.

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