High-protein full-nutrient solid beverage and preparation method thereof

Abstract

The invention relates to a high-protein full-nutrient solid beverage and a preparation method thereof, and belongs to the technical field of food. The food is prepared from the protein compound, the compound extracting solution, the powdered oil, the nutritional supplement, the potassium citrate, the xanthan gum and the sucrose fatty acid ester, is rich in various amino acids and nutritional ingredients required by a human body, and is treated by a specific process, so that the absorption effect of the protein compound, the nutritional supplement and the like is further improved; therefore, rich protein sources and comprehensive nutritional supplements are provided for human bodies.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and relates to a high-protein complete nutrition solid beverage and a preparation method thereof. Background Art

[0002] As people's living standards improve and their health awareness strengthens, the demand for nutritional supplements has become more diverse. Functional solid beverages have gradually entered the public eye due to their convenience in carrying, storage and brewing. However, most protein solid beverages on the market are based on a single protein source and have relatively simple nutritional ingredients, which cannot meet people's needs for comprehensive nutrition. Summary of the invention

[0003] The purpose of the present invention is to provide a high-protein complete nutrition solid beverage and a preparation method thereof. The present invention is prepared from a protein complex, a composite extract, powdered oil, a nutritional supplement, potassium citrate, xanthan gum and sucrose fatty acid esters, and is rich in a variety of amino acids and nutrients required by the human body. Through specific process treatment, the absorption effect of the protein complex and the nutritional supplement is further improved, thereby providing a rich protein source and comprehensive nutritional supplement for the human body.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A high-protein complete nutrition solid beverage comprises the following components by weight: 58-70 parts of protein complex, 8-14 parts of composite extract, 12-20 parts of powdered oil, 4-8 parts of nutritional supplement, 0.5-1.5 parts of potassium citrate, 0.2-0.5 parts of xanthan gum, and 0.5-1.5 parts of sucrose fatty acid ester.

[0006] Furthermore, the preparation method of the protein complex is: first pour whey protein, yellow rice protein peptide and moringa seed protein peptide into a reactor, stir at a speed of 20-30rmp for 5-15min, then add sodium caseinate, stir at a speed of 50-60rmp for 10-20min, finally add resistant dextrin and wheat oligopeptide, stir at a speed of 70-80rmp for 20-30min, to obtain a protein complex; the protein complex includes the following components by weight: 10-15 parts of whey protein, 18-26 parts of yellow rice protein peptide, 6-10 parts of moringa seed protein peptide, 10-20 parts of resistant dextrin, 8-15 parts of sodium caseinate, and 2-5 parts of wheat oligopeptide.

[0007] Furthermore, the method for preparing the yellow rice protein peptide comprises the following steps:

[0008] a1. Wash the yellow rice, dry it, crush it to 50-70 mesh, mix it with sodium hydroxide solution in a mass ratio of 1:8-12, stir it at 100-200rmp for 1-3h, stir it at 10-30rmp and add citric acid to adjust the pH to 4.2-4.8, stop stirring, centrifuge and take the precipitate;

[0009] a2. The precipitate was mixed with deionized water in a mass ratio of 1:5-10, incubated in a water bath at 40-50 ° C for 10-20 min, and then papain was added, sodium hydroxide solution and citric acid were added dropwise to adjust the pH to 6.3-6.7, and stirred at 60-100 rpm for 3-5 h to obtain an enzymatic solution;

[0010] a3. The enzymatic hydrolysate is centrifuged, ultrafiltered, eluted with a cation exchange resin column, the eluate is collected, and vacuum freeze-dried to obtain yellow rice protein peptide.

[0011] Furthermore, the mass of the papain accounts for 2.2-3.8% of the mass of the precipitate; the concentration of the sodium hydroxide solution is 0.1-0.5 mol / L; and the concentration of the citric acid is 0.1-0.5 mol / L.

[0012] Furthermore, the centrifugal parameters in the step a1 are: centrifugal speed of 3000-5000 rpm, and centrifugal time of 10-14 min; the centrifugal parameters in the step a3 are: centrifugal speed of 4500-5500 rpm, and centrifugal time of 16-20 min; the ultrafiltration in the step a3 uses an ultrafiltration membrane with a pore size of 3000-4000D.

[0013] Furthermore, the cation exchange resin column is a sodium carboxymethylcellulose cation exchange resin column; the cation exchange resin column is eluted by first washing the resin column with 3 times the volume of the resin column Tris-HCl buffer (I), then passing the filtrate, and then washing the resin column with 3 times the volume of the resin column Tris-HCl buffer (II).

[0014] Furthermore, the Tris-HCl buffer (I) is a Tris-HCl buffer with a pH of 6.8-7.2 and a concentration of 0.05-0.15 mol / L; the Tris-HCl buffer (II) is a Tris-HCl buffer with a pH of 7.5-8.5 and a concentration of 0.2-0.4 mol / L; and the elution rate of the elution is 0.5-1.5 BV / h.

[0015] Furthermore, the composite extract is obtained by mixing the composite extract and deionized water in a mass ratio of 1:1.2-1.8; the composite extract includes the following components in parts by weight: 3.3-4.8 parts of yam extract, 1.8-3 parts of pumpkin seed extract, 2.5-3.5 parts of spinach extract, and 0.8-1.7 parts of lemon extract.

[0016] Furthermore, the nutritional supplement includes the following components by weight: 1.5-3 parts of vitamin A, 8-10 parts of vitamin C, 0.1-0.2 parts of vitamin D, 4-6 parts of vitamin E, 1.1-2 parts of vitamin B1, 1.1-2 parts of vitamin B2, 2.4-4 parts of vitamin B5, 0.4-1 parts of vitamin B6, and 0.1-0.2 parts of vitamin B12.

[0017] Furthermore, the preparation method of the high-protein complete nutrition solid beverage comprises the following steps:

[0018] b1. Add the protein complex, powdered oil and nutritional supplements to the composite extract and stir at a speed of 20-30rmp to obtain material A;

[0019] b2. The xanthan gum and deionized water were mixed in a mass ratio of 1:1.5-2.5, stirred evenly, and then added to material A and stirred at a speed of 50-60rmp to obtain material B;

[0020] b3. Add potassium citrate and sucrose fatty acid ester to material B, set the ultrasonic frequency to 20-40kHz, the ultrasonic power to 50-100W, ultrasonic 10-20min, and continue stirring for 2-3h to obtain material C;

[0021] b4. Set the spray dryer inlet air temperature to 150-170℃, the peristaltic pump speed to 10-20rmp, and the inlet air flow rate to 30-40m 3 / h, spray-dry material C, and grind through a 60-100 mesh sieve to obtain a high-protein, complete-nutrition solid beverage.

[0022] Beneficial effects of the present invention:

[0023] (1) The present invention adds a variety of protein raw materials, which are rich in various essential amino acids and nutrients for the human body. The yellow rice protein peptide can preferentially improve the protein utilization rate after multiple process treatments. The processed protein has a small molecular weight and is easily absorbed by the human body. In addition, the sodium caseinate contains phosphorus-calcium binding protein, which can provide the human body with abundant minerals such as calcium and phosphorus. The digestion and absorption are relatively slow, and it can continuously provide amino acids to the human body to maintain the body's nitrogen balance. Each group synergistically provides a rich source of protein for the human body.

[0024] (2) The present invention also adds a composite extract and a nutritional supplement. The composite extract is prepared by a variety of extracts and is rich in a variety of nutrients, which helps to provide comprehensive nutritional supplements for the human body. In addition, the lemon extract therein can better neutralize the unpleasant flavors produced by Moringa seed protein peptides and other extracts; the nutrients in different extracts work synergistically with each other to further improve the absorption effect of protein complexes and nutritional supplements. DETAILED DESCRIPTION

[0025] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0026] The whey proteins in all the embodiments and comparative examples of the present invention were purchased directly from the market, and were all purchased from Zhengzhou Yuhe Food Additive Co., Ltd.

[0027] Moringa seed protein peptides were purchased directly from the market and were purchased from Shaanxi Mufei Biotechnology Co., Ltd.

[0028] Sodium caseinate was purchased directly from the market, and was purchased from Sichuan Youlun Duohui Biotechnology Co., Ltd.

[0029] The resistant dextrins were all purchased directly from the market, and were all purchased from Xi'an Lavia Biotechnology Co., Ltd.

[0030] Wheat oligopeptides were purchased directly from the market and were purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0031] The yam extracts were all purchased directly from the market and were purchased from Shaanxi Kangjun Biotechnology Co., Ltd.

[0032] Pumpkin seed extracts were purchased directly from the market, and were purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0033] All spinach extracts were purchased directly from the market, and were purchased from Shaanxi Angxu Biotechnology Co., Ltd.

[0034] Lemon extracts were purchased directly from the market and were purchased from Baoji Liupanyun Biotechnology Co., Ltd.

[0035] Example 1

[0036] A high-protein complete nutrition solid beverage. The high-protein complete nutrition solid beverage of this embodiment comprises the following components by weight: 58 parts of protein complex, 8 parts of composite extract, 12 parts of powdered oil, 4 parts of nutritional supplement, 0.5 parts of potassium citrate, 0.2 parts of xanthan gum, and 0.5 parts of sucrose fatty acid ester.

[0037] The preparation method of the protein complex of the present embodiment is as follows: first, whey protein, yellow rice protein peptide and moringa seed protein peptide are poured into a reactor, stirred at a speed of 20 rpm for 5 min, then sodium caseinate is added, stirred at a speed of 50 rpm for 10 min, and finally, resistant dextrin and wheat oligopeptide are added, stirred at a speed of 70 rpm for 20 min to obtain a protein complex; the protein complex of the present embodiment comprises the following components by weight: 10 parts of whey protein, 18 parts of yellow rice protein peptide, 6 parts of moringa seed protein peptide, 10 parts of resistant dextrin, 8 parts of sodium caseinate, and 2 parts of wheat oligopeptide.

[0038] The preparation method of yellow rice protein peptide of this embodiment comprises the following steps:

[0039] a1. Wash the yellow rice, dry it, crush it to 50 mesh, mix it with sodium hydroxide solution in a mass ratio of 1:8, stir it at 100 rpm for 1h, stir it at 10 rpm and add citric acid to adjust the pH to 4.2, stop stirring, centrifuge and take the precipitate;

[0040] a2. The precipitate was mixed with deionized water in a mass ratio of 1:5, incubated in a water bath at 40°C for 10 min, and then papain was added. Sodium hydroxide solution and citric acid were added dropwise to adjust the pH to 6.3, and stirred at 60 rpm for 3 h to obtain an enzymatic solution.

[0041] a3. The enzymatic hydrolysate is centrifuged, ultrafiltered, eluted with a cation exchange resin column, the eluate is collected, and vacuum freeze-dried to obtain yellow rice protein peptide.

[0042] The mass of papain in this embodiment accounts for 2.2% of the mass of the precipitate; the concentration of the sodium hydroxide solution in this embodiment is 0.1 mol / L; the concentration of the citric acid in this embodiment is 0.1 mol / L.

[0043] The centrifugal parameters in step a1 of this embodiment are: centrifugal speed of 3000 rpm, centrifugal time of 10 min; the centrifugal parameters in step a3 of this embodiment are: centrifugal speed of 4500 rpm, centrifugal time of 16 min; the ultrafiltration in step a3 of this embodiment uses an ultrafiltration membrane with a pore size of 3000D.

[0044] The cation exchange resin column of the present embodiment is a sodium carboxymethylcellulose cation exchange resin column; the cation exchange resin column of the present embodiment is eluted by first washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (I), then passing the filtrate, and then washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (II).

[0045] The Tris-HCl buffer (I) of the present embodiment is a Tris-HCl buffer having a pH of 6.8 and a concentration of 0.05 mol / L; the Tris-HCl buffer (II) of the present embodiment is a Tris-HCl buffer having a pH of 7.5 and a concentration of 0.2 mol / L; the elution rate of the present embodiment is 0.5 BV / h.

[0046] The composite extract of this embodiment is obtained by mixing the composite extract and deionized water in a mass ratio of 1:1.2; the composite extract of this embodiment includes the following components in parts by weight: 3.3 parts of yam extract, 1.8 parts of pumpkin seed extract, 2.5 parts of spinach extract, and 0.8 parts of lemon extract.

[0047] The nutritional supplement of this embodiment includes the following components by weight: 1.5 parts of vitamin A, 8 parts of vitamin C, 0.1 parts of vitamin D, 4 parts of vitamin E, 1.1 parts of vitamin B1, 1.1 parts of vitamin B2, 2.4 parts of vitamin B5, 0.4 parts of vitamin B6, and 0.1 parts of vitamin B12.

[0048] The preparation method of the high-protein complete nutrition solid beverage of this embodiment comprises the following steps:

[0049] b1. Add the protein complex, powdered fat and nutritional supplements to the composite extract and stir at a speed of 20rmp to obtain material A;

[0050] b2. The xanthan gum and deionized water were mixed in a mass ratio of 1:1.5, stirred evenly, and then added to material A and stirred at a speed of 50rmp to obtain material B;

[0051] b3. Potassium citrate and sucrose fatty acid ester were added to material B, the ultrasonic frequency was set to 20kHz, the ultrasonic power was 50W, the ultrasound was performed for 10min, and then stirring was continued for 2h to obtain material C;

[0052] b4. Set the spray dryer inlet air temperature to 150°C, the peristaltic pump speed to 10 rpm, and the inlet air flow rate to 30 m / s. 3 / h, spray-dry material C, and grind it through a 60-mesh sieve to obtain a high-protein and complete-nutrition solid beverage.

[0053] Example 2

[0054] A high-protein complete nutrition solid beverage. The high-protein complete nutrition solid beverage of this embodiment comprises the following components by weight: 70 parts of protein complex, 14 parts of composite extract, 20 parts of powdered oil, 8 parts of nutritional supplement, 1.5 parts of potassium citrate, 0.5 parts of xanthan gum, and 1.5 parts of sucrose fatty acid ester.

[0055] The preparation method of the protein complex of the present embodiment is as follows: first pour whey protein, yellow rice protein peptide and moringa seed protein peptide into a reactor, stir at 30 rpm for 15 min, then add sodium caseinate, stir at 60 rpm for 20 min, finally add resistant dextrin and wheat oligopeptide, stir at 80 rpm for 30 min to obtain a protein complex; the protein complex of the present embodiment includes the following components by weight: 15 parts of whey protein, 26 parts of yellow rice protein peptide, 10 parts of moringa seed protein peptide, 20 parts of resistant dextrin, 15 parts of sodium caseinate, and 5 parts of wheat oligopeptide.

[0056] The preparation method of yellow rice protein peptide of this embodiment comprises the following steps:

[0057] a1. Wash the yellow rice, dry it, crush it to 70 mesh, mix it with sodium hydroxide solution in a mass ratio of 1:12, stir it at 200 rpm for 3h, stir it at 30 rpm and add citric acid to adjust the pH to 4.8, stop stirring, centrifuge and take the precipitate;

[0058] a2. The precipitate was mixed with deionized water in a mass ratio of 1:10, incubated in a water bath at 50°C for 20 min, and then papain was added. Sodium hydroxide solution and citric acid were added dropwise to adjust the pH to 6.7, and stirred at 100 rpm for 5 h to obtain an enzymatic solution.

[0059] a3. The enzymatic hydrolysate is centrifuged, ultrafiltered, eluted with a cation exchange resin column, the eluate is collected, and vacuum freeze-dried to obtain yellow rice protein peptide.

[0060] The mass of papain in this embodiment accounts for 3.8% of the mass of the precipitate; the concentration of the sodium hydroxide solution in this embodiment is 0.5 mol / L; the concentration of the citric acid in this embodiment is 0.5 mol / L.

[0061] The centrifugal parameters in step a1 of this embodiment are: centrifugal speed of 5000 rpm, centrifugal time of 14 min; the centrifugal parameters in step a3 of this embodiment are: centrifugal speed of 5500 rpm, centrifugal time of 20 min; the ultrafiltration in step a3 of this embodiment uses an ultrafiltration membrane with a pore size of 4000D.

[0062] The cation exchange resin column of the present embodiment is a sodium carboxymethylcellulose cation exchange resin column; the cation exchange resin column of the present embodiment is eluted by first washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (I), then passing the filtrate, and then washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (II).

[0063] The Tris-HCl buffer (I) of the present embodiment is a Tris-HCl buffer having a pH of 7.2 and a concentration of 0.15 mol / L; the Tris-HCl buffer (II) of the present embodiment is a Tris-HCl buffer having a pH of 8.5 and a concentration of 0.4 mol / L; the elution rate of the present embodiment is 1.5 BV / h.

[0064] The composite extract of this embodiment is obtained by mixing the composite extract and deionized water in a mass ratio of 1:1.8; the composite extract of this embodiment includes the following components in parts by weight: 4.8 parts of yam extract, 3 parts of pumpkin seed extract, 3.5 parts of spinach extract, and 1.7 parts of lemon extract.

[0065] The nutritional supplement of this embodiment includes the following components by weight: 3 parts of vitamin A, 10 parts of vitamin C, 0.2 parts of vitamin D, 6 parts of vitamin E, 2 parts of vitamin B1, 2 parts of vitamin B2, 4 parts of vitamin B5, 1 part of vitamin B6, and 0.2 parts of vitamin B12.

[0066] The preparation method of the high-protein complete nutrition solid beverage of this embodiment comprises the following steps:

[0067] b1. Add the protein complex, powdered oil and nutritional supplements to the composite extract and stir at a speed of 30rmp to obtain material A;

[0068] b2. The xanthan gum and deionized water were mixed in a mass ratio of 1:2.5, stirred evenly, and then added to material A and stirred at a speed of 60rmp to obtain material B;

[0069] b3. Potassium citrate and sucrose fatty acid ester were added to material B, the ultrasonic frequency was set to 40kHz, the ultrasonic power was 100W, the ultrasound was continued for 20min, and then stirred for 3h to obtain material C;

[0070] b4. Set the spray dryer inlet air temperature to 170°C, the peristaltic pump speed to 20 rpm, and the inlet air flow rate to 40 m / s. 3 / h, spray-dry material C, and grind it through a 100-mesh sieve to obtain a high-protein, complete-nutrition solid beverage.

[0071] Example 3

[0072] A high-protein complete nutrition solid beverage. The high-protein complete nutrition solid beverage of this embodiment comprises the following components by weight: 64 parts of protein complex, 11 parts of composite extract, 16 parts of powdered oil, 6 parts of nutritional supplements, 1 part of potassium citrate, 0.35 parts of xanthan gum, and 1 part of sucrose fatty acid ester.

[0073] The preparation method of the protein complex of the present embodiment is as follows: first pour whey protein, yellow rice protein peptide and moringa seed protein peptide into a reactor, stir at 25 rpm for 10 min, then add sodium caseinate, stir at 55 rpm for 15 min, finally add resistant dextrin and wheat oligopeptide, stir at 75 rpm for 25 min to obtain a protein complex; the protein complex of the present embodiment includes the following components by weight: 12.5 parts of whey protein, 22 parts of yellow rice protein peptide, 8 parts of moringa seed protein peptide, 15 parts of resistant dextrin, 11.5 parts of sodium caseinate, and 3.5 parts of wheat oligopeptide.

[0074] The preparation method of yellow rice protein peptide of this embodiment comprises the following steps:

[0075] a1. Wash the yellow rice, dry it, crush it to 60 mesh, mix it with sodium hydroxide solution in a mass ratio of 1:10, stir it at 150rmp for 2h, stir it at 20rmp and add citric acid to adjust the pH to 4.5, stop stirring, centrifuge and take the precipitate;

[0076] a2. The precipitate was mixed with deionized water in a mass ratio of 1:7.5, incubated in a water bath at 45°C for 15 min, and then papain was added. Sodium hydroxide solution and citric acid were added dropwise to adjust the pH to 6.5, and stirred at 80 rpm for 4 h to obtain an enzymatic solution.

[0077] a3. The enzymatic hydrolysate is centrifuged, ultrafiltered, eluted with a cation exchange resin column, the eluate is collected, and vacuum freeze-dried to obtain yellow rice protein peptide.

[0078] The mass of papain in this embodiment accounts for 3% of the mass of the precipitate; the concentration of the sodium hydroxide solution in this embodiment is 0.3 mol / L; the concentration of the citric acid in this embodiment is 0.3 mol / L.

[0079] The centrifugal parameters in step a1 of this embodiment are: centrifugal speed of 4000 rpm, centrifugal time of 12 min; the centrifugal parameters in step a3 of this embodiment are: centrifugal speed of 5000 rpm, centrifugal time of 18 min; the ultrafiltration in step a3 of this embodiment uses an ultrafiltration membrane with a pore size of 3500D.

[0080] The cation exchange resin column of the present embodiment is a sodium carboxymethylcellulose cation exchange resin column; the cation exchange resin column of the present embodiment is eluted by first washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (I), then passing the filtrate, and then washing the resin column with 3 times the volume of the resin column of Tris-HCl buffer (II).

[0081] The Tris-HCl buffer (I) of the present embodiment is a Tris-HCl buffer having a pH of 7 and a concentration of 0.1 mol / L; the Tris-HCl buffer (II) of the present embodiment is a Tris-HCl buffer having a pH of 8 and a concentration of 0.3 mol / L; the elution rate of the present embodiment is 1 BV / h.

[0082] The composite extract of this embodiment is obtained by mixing the composite extract and deionized water in a mass ratio of 1:1.5; the composite extract of this embodiment includes the following components in parts by weight: 4.05 parts of yam extract, 2.4 parts of pumpkin seed extract, 3 parts of spinach extract, and 1.25 parts of lemon extract.

[0083] The nutritional supplement of this embodiment includes the following components by weight: 2.25 parts of vitamin A, 9 parts of vitamin C, 0.15 parts of vitamin D, 4-6 parts of vitamin E, 1.55 parts of vitamin B1, 1.55 parts of vitamin B2, 3.2 parts of vitamin B5, 0.7 parts of vitamin B6, and 0.15 parts of vitamin B12.

[0084] The preparation method of the high-protein complete nutrition solid beverage of this embodiment comprises the following steps:

[0085] b1. Add the protein complex, powdered oil and nutritional supplements to the composite extract and stir at a speed of 20-30rmp to obtain material A;

[0086] b2. The xanthan gum and deionized water were mixed in a mass ratio of 1:2, stirred evenly, and then added to material A and stirred at a speed of 55rmp to obtain material B;

[0087] b3. Potassium citrate and sucrose fatty acid ester were added to material B, the ultrasonic frequency was set to 30kHz, the ultrasonic power was 75W, the ultrasound was performed for 15min, and then stirring was continued for 2.5h to obtain material C;

[0088] b4. Set the spray dryer inlet air temperature to 160°C, the peristaltic pump speed to 15 rpm, and the inlet air flow rate to 35 m / s. 3 / h, spray-dry material C, and grind it through an 80-mesh sieve to obtain a high-protein and complete-nutrition solid beverage.

[0089] Comparative Example 1

[0090] On the basis of Example 3, the yellow rice protein peptide was removed and replaced with an equal weight of resistant dextrin, and other conditions were consistent with Example 3.

[0091] Comparative Example 2

[0092] On the basis of Example 3, the elution treatment in the yellow rice protein peptide preparation step was removed, and other conditions were consistent with Example 3.

[0093] Comparative Example 3

[0094] On the basis of Example 3, the preparation steps of yellow rice protein peptide are changed to wash, dry and crush yellow rice to 60 mesh, mix it with sodium hydroxide solution at a mass ratio of 1:10, stir it at a speed of 150 rpm for 2 hours, stir it at a speed of 20 rpm and add citric acid to adjust the pH to 4.5, stop stirring, centrifuge, take the precipitate, mix the precipitate with deionized water at a mass ratio of 1:7.5, bathe it in a water bath at 45°C for 15 minutes, centrifuge, take the supernatant, add sodium hydroxide solution and citric acid to adjust the pH to the isoelectric point of yellow rice protein, i.e. 4.7, centrifuge for 20 minutes after protein coagulation, and vacuum freeze-dry to obtain yellow rice protein peptide. The centrifugal speed of this comparative example is 4000 rpm.

[0095] The high-protein complete nutrition solid beverages prepared in the above examples and comparative examples were used as samples, and their protein contents were determined according to GB5009.5-2010. The protein contents per 100 g of the samples were recorded as shown in Table 1 below.

[0096] Table 1. Protein content test results

[0097]

[0098]

[0099] As shown in Table 1, the solid beverages prepared by Examples 1-3 of this scheme have a higher protein content, while the yellow rice protein peptide is removed in Comparative Example 1 and replaced with resistant dextrin, resulting in a lower protein content; Comparative Example 2 removes the elution step, and although the measured protein content increases, the protein purity is lower than that of Examples 1-3, which may affect the absorption effect of the protein by the human body; Comparative Example 3 changes the extraction method of the yellow rice protein peptide, which is lower than the protein content prepared by the preparation method of this scheme. In addition, the present invention also adds ingredients such as a composite extract and a nutritional supplement, which are rich in a variety of nutrients and trace elements, and can provide a comprehensive nutritional supplement for the human body, indicating that the solid beverage prepared by the present invention has a high protein content and comprehensive nutrition.

[0100] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A high-protein complete nutrition solid beverage, characterized by: The high-protein complete nutrition solid beverage comprises the following components by weight: 58-70 parts of protein complex, 8-14 parts of composite extract, 12-20 parts of powdered oil, 4-8 parts of nutritional supplement, 0.5-1.5 parts of potassium citrate, 0.2-0.5 parts of xanthan gum, and 0.5-1.5 parts of sucrose fatty acid ester.

2. A high-protein complete nutrition solid beverage according to claim 1, characterized in that: The preparation method of the protein complex is as follows: firstly, whey protein, yellow rice protein peptide and moringa seed protein peptide are poured into a reaction kettle, stirred at a speed of 20-30 rpm for 5-15 min, then sodium caseinate is added, stirred at a speed of 50-60 rpm for 10-20 min, and finally, resistant dextrin and wheat oligopeptide are added, stirred at a speed of 70-80 rpm for 20-30 min, so as to obtain the protein complex; the protein complex comprises the following components by weight: 10-15 parts of whey protein, 18-26 parts of yellow rice protein peptide, 6-10 parts of moringa seed protein peptide, 10-20 parts of resistant dextrin, 8-15 parts of sodium caseinate and 2-5 parts of wheat oligopeptide.

3. A high-protein complete nutrition solid beverage according to claim 2, characterized in that: The preparation method of the yellow rice protein peptide comprises the following steps: a1. Wash the yellow rice, dry it, crush it to 50-70 mesh, mix it with sodium hydroxide solution in a mass ratio of 1:8-12, stir it at 100-200rmp for 1-3h, stir it at 10-30rmp and add citric acid to adjust the pH to 4.2-4.8, stop stirring, centrifuge and take the precipitate; a2. The precipitate was mixed with deionized water in a mass ratio of 1:5-10, incubated in a water bath at 40-50 ° C for 10-20 min, and then papain was added, sodium hydroxide solution and citric acid were added dropwise to adjust the pH to 6.3-6.7, and stirred at 60-100 rpm for 3-5 h to obtain an enzymatic solution; a3. The enzymatic hydrolysate is centrifuged, ultrafiltered, eluted with a cation exchange resin column, the eluate is collected, and vacuum freeze-dried to obtain yellow rice protein peptide.

4. A high-protein complete nutrition solid beverage according to claim 3, characterized in that: The mass of the papain accounts for 2.2-3.8% of the mass of the precipitate; the concentration of the sodium hydroxide solution is 0.1-0.5 mol / L; and the concentration of the citric acid is 0.1-0.5 mol / L.

5. A high-protein complete nutrition solid beverage according to claim 3, characterized in that: The centrifugal parameters in step a1 are: centrifugal speed of 3000-5000 rpm, centrifugal time of 10-14 min; the centrifugal parameters in step a3 are: centrifugal speed of 4500-5500 rpm, centrifugal time of 16-20 min; the ultrafiltration in step a3 uses an ultrafiltration membrane with a pore size of 3000-4000D.

6. A high-protein complete nutrition solid beverage according to claim 3, characterized in that: The cation exchange resin column is a sodium carboxymethylcellulose cation exchange resin column; the cation exchange resin column is eluted by first washing the resin column with a Tris-HCl buffer (I) of 3 times the volume of the resin column, then passing a filtrate, and then washing the resin column with a Tris-HCl buffer (II) of 3 times the volume of the resin column.

7. The method for preparing a high-protein complete nutrition solid beverage according to claim 6, characterized in that: The Tris-HCl buffer (I) is a Tris-HCl buffer with a pH of 6.8-7.2 and a concentration of 0.05-0.15 mol / L; the Tris-HCl buffer (II) is a Tris-HCl buffer with a pH of 7.5-8.5 and a concentration of 0.2-0.4 mol / L; and the elution rate of the elution is 0.5-1.5 BV / h.

8. The high-protein complete nutrition solid beverage according to claim 1, characterized in that: The composite extract is obtained by mixing the composite extract and deionized water in a mass ratio of 1:1.2-1.8; the composite extract comprises the following components in parts by weight: 3.3-4.8 parts of yam extract, 1.8-3 parts of pumpkin seed extract, 2.5-3.5 parts of spinach extract, and 0.8-1.7 parts of lemon extract.

9. A high-protein complete nutrition solid beverage according to claim 1, characterized in that: The nutritional supplement comprises the following components by weight: 1.5-3 parts of vitamin A, 8-10 parts of vitamin C, 0.1-0.2 parts of vitamin D, 4-6 parts of vitamin E, 1.1-2 parts of vitamin B1, 1.1-2 parts of vitamin B2, 2.4-4 parts of vitamin B5, 0.4-1 parts of vitamin B6, and 0.1-0.2 parts of vitamin B12.

10. A high-protein complete nutrition solid beverage according to any one of claims 1 to 9, characterized in that: The preparation method of the high-protein complete nutrition solid beverage comprises the following steps: b1. Add the protein complex, powdered oil and nutritional supplements to the composite extract and stir at a speed of 20-30rmp to obtain material A; b2. The xanthan gum and deionized water were mixed in a mass ratio of 1:1.5-2.5, stirred evenly, and then added to material A and stirred at a speed of 50-60rmp to obtain material B; b3. Add potassium citrate and sucrose fatty acid ester to material B, set the ultrasonic frequency to 20-40kHz, the ultrasonic power to 50-100W, ultrasonic 10-20min, and continue stirring for 2-3h to obtain material C; b4. Set the spray dryer inlet air temperature to 150-170℃, the peristaltic pump speed to 10-20rmp, and the inlet air flow rate to 30-40m 3 / h, spray-dry material C, and grind through a 60-100 mesh sieve to obtain a high-protein, complete-nutrition solid beverage.

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