Thallus culture device

By designing the structure of the porous carrier and the supplementary tube in the bacterial culture device, the nutrient solution is directly replenished into the porous carrier, which solves the problem of insufficient nutrient solution replenishment and promotes the growth of the bacterial cells.

CN119325862APending Publication Date: 2025-01-21張瑞能
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Patent Information

Application Number
CN202310900210.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-07-21
Publication Date
2025-01-21

AI Technical Summary

Technical Problem

In the existing bacterial culture device, the nutrient solution is insufficient, resulting in the bacteria being unable to fully absorb water and nutrients, resulting in a decrease in growth rate or stagnation.

Method used

A bacterial culture device is designed, which includes a porous carrier and a supplementary tube. The nutrient solution is directly supplemented into the porous carrier through the supplementary tube and the supplementary hole, ensuring that the nutrient solution can be evenly diffused and absorbed by the bacterial cells.

Benefits of technology

It solves the problem that the nutrient solution cannot be completely absorbed by the bacteria, promotes the continuous growth of the bacteria, and allows the nutrient input to be adjusted according to the growth conditions.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention provides a thallus culture device. The thallus culture device comprises a box body, a porous carrier and a supplementing pipe, the box body is provided with an accommodating space; the porous carrier is arranged in the containing space, the porous carrier is provided with a supplementing hole, the supplementing hole penetrates through the porous carrier in the horizontal direction, and the length of the supplementing hole in the horizontal direction is smaller than that of the porous carrier in the horizontal direction; one end of the supplementing pipe is inserted into the supplementing hole. Therefore, a user can pour a nutrient solution into the supplementing pipe, the nutrient solution is directly supplemented into the porous carrier from the supplementing pipe through the supplementing holes and is diffused in the porous carrier, so that the problem that the nutrient solution cannot be completely absorbed by thalli is solved, and the thalli are promoted to continuously grow.
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Description

Technical Field

[0001] The present invention relates to a culture device, in particular to a bacterial culture device. Background Art

[0002] In the prior art, fungi are usually cultivated by placing the fungi on a carrier, and placing the carrier containing the fungi in a culture environment. The culturer then regularly adds water and nutrient solution to the carrier, so that the fungi absorb water and nutrients and continue to grow.

[0003] During the growth process, the bacteria will gradually cover the carrier, so that when the culturer replenishes water and nutrient solution, the water and nutrient solution will be blocked by the bacteria and cannot be completely replenished into the carrier, making it impossible for the bacteria growing in the carrier to absorb water and nutrients, resulting in a decrease in the growth rate of the bacteria or even stagnation. Summary of the invention

[0004] To solve the above problems, the present invention discloses a bacterial culture device, which allows the user to pour nutrient solution into a replenishing tube, and the nutrient solution is directly replenished from the replenishing tube to the porous carrier through the replenishing holes, thereby solving the problem that the bacteria growing in the carrier cannot absorb water and nutrients.

[0005] To achieve the above-mentioned purpose, an embodiment of the present invention provides a bacterial culture device, which includes a box body, a porous carrier and a supplementary tube. The box body has a containing space; the porous carrier is arranged in the containing space, and the porous carrier has a supplementary hole, which is arranged in a horizontal direction through the porous carrier, and the length of the supplementary hole in the horizontal direction is less than the length of the porous carrier in the horizontal direction; one end of the supplementary tube is inserted into the supplementary hole.

[0006] In another embodiment of the present invention, the diameter of the supplementary hole is between 14 and 19 mm.

[0007] In another embodiment of the present invention, the ratio of the length of the supplementary hole along the horizontal direction to the length of the porous carrier along the horizontal direction is between 3:5 and 4:5.

[0008] In another embodiment of the present invention, the supplementary tube has a connecting section and a bending section, the connecting section is inserted into the supplementary hole, and the bending section is exposed in the accommodating space.

[0009] In another embodiment of the present invention, the connecting section has a connecting length in the supplementary hole, and the connecting length is between 5 and 10 centimeters.

[0010] In another embodiment of the present invention, a fixed plug is further included, and the fixed plug is sleeved on the periphery of the supplementary pipe.

[0011] In another embodiment of the present invention, the fixed plug has a supporting portion and a plugging portion, the supporting portion is supported against the side edge of the porous carrier, and the plugging portion is disposed in the supplementary hole.

[0012] In another embodiment of the present invention, a receiving groove is recessed in the receiving space, and the receiving groove is used to place the porous carrier. The receiving space is provided with an inclined guide portion at the periphery of the receiving groove.

[0013] In another embodiment of the present invention, a cover is further included, and the cover is selectively covered on the box body.

[0014] In another embodiment of the present invention, the cover body has a through hole and a breathable member, the position of the through hole corresponds to the opening of the replenishing tube, and the breathable member is selectively covered on the through hole.

[0015] In this way, the present invention enables the user to pour the nutrient solution into the replenishing tube, so that the nutrient solution is directly replenished from the replenishing tube through the replenishing hole into the porous carrier and diffuses in the porous carrier, thereby solving the problem that the nutrient solution cannot be completely absorbed by the bacteria and promoting the bacteria to continue to grow.

[0016] Furthermore, the user can also input substances required for bacterial growth (such as oxygen, inducers, etc.) directly into the porous carrier from the replenishment tube through the replenishment hole according to the growth conditions of the bacteria, so that the bacteria can grow in the best growth environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of the appearance of the bacterial culture device according to an embodiment of the present invention.

[0018] Figure 2 It is a cross-sectional schematic diagram of the bacterial culture device according to an embodiment of the present invention, used to indicate the connection position of the supplementary tube and the length of the supplementary hole.

[0019] Figure 3 It is a cross-sectional schematic diagram of the bacterial culture device according to an embodiment of the present invention, which is used to indicate that the nutrient solution can be replenished into the carrier through the replenishing tube and the replenishing hole.

[0020] Figure 4 It is a three-dimensional schematic diagram of the appearance connection between the porous carrier and the supplementary tube of an embodiment of the present invention.

[0021] Figure 5 It is a schematic diagram of the decomposition of the porous carrier and the supplementary tube according to an embodiment of the present invention.

[0022] In the figure: 100, bacterial culture device; 200, bacteria; 10, box body; 11, accommodating space; 12, accommodating groove; 13, guide part; 20, porous carrier; 21, supplementary hole; 30, supplementary pipe; 31, connecting section; 32, bending section; 40, fixed plug; 41, abutment part; 42, plug; 50, cover body; 51, perforation; 52, breathable part; L1, length; L2, length; L3, connection length; X, horizontal direction. DETAILED DESCRIPTION

[0023] In order to explain the central idea of ​​the present invention, a specific embodiment is now used. Various objects in the embodiment are drawn in proportions suitable for enumeration and description, rather than in proportions of actual components.

[0024] See also Figures 1 to 5 As shown, a bacterial cell culture device 100 according to an embodiment of the present invention is disclosed, which is used to culture a bacterial cell 200, and the bacterial cell 200 is a fruiting body of Antrodia cinnamomea, but it is not limited to this. In other embodiments, the bacterial cell 200 can also be any edible fungus. The bacterial cell culture device 100 includes a box body 10, a porous carrier 20 and a supplementary tube 30. In addition, the bacterial cell culture device 100 can also include a fixed plug 40 and a cover 50.

[0025] The box body 10 has a receiving space 11, and the receiving space 11 is used to place the porous carrier 20. Figures 1 to 3 As shown, in the embodiment of the present invention, a receiving groove 12 is concavely provided at the bottom of the receiving space 11, and the receiving groove 12 is used to place the porous carrier 20, so that the porous carrier 20 can be stuck in the receiving groove 12 to prevent the porous carrier 20 from shaking too much in the box body 10; the receiving space 11 is provided with an inclined guide portion 13 at the periphery of the receiving groove 12, whereby when the moisture in the box body 10 condenses into water at the cover body 50 and the box wall of the box body 10, the water can flow into the receiving groove 12 through the guide portion 13, so that the porous carrier 20 can continuously replenish water. Among them, the relative humidity in the box body 10 is between 85% and 90%, thereby providing the best growth environment for the bacteria 200.

[0026] The porous carrier 20 is disposed in the accommodating space 11 of the box body 10. The porous carrier 20 has a supplementary hole 21. The supplementary hole 21 is disposed in the porous carrier 20 along a horizontal direction X. The length L1 of the supplementary hole 21 along the horizontal direction X is less than the length L2 of the porous carrier 20 along the horizontal direction X. Figures 1 to 3As shown, in the embodiment of the present invention, the ratio of the length L1 of the supplementary hole 21 along the horizontal direction X to the length L2 of the porous carrier 20 along the horizontal direction X is between 3:5 and 4:5, and the aperture of the supplementary hole 21 is between 14 and 19 mm, so that when the user pours the nutrient solution into the supplementary hole 21, the nutrient solution can flow completely and evenly to all parts of the porous carrier 20, and the nutrient solution can be completely absorbed by the bacteria 200. The horizontal direction X is the length direction of the porous carrier 20.

[0027] One end of the replenishing tube 30 is inserted into the replenishing hole 21, so that the user can pour the nutrient solution into the replenishing hole 21 through the replenishing tube 30. Figures 1 to 3 As shown, in the embodiment of the present invention, the supplementing tube 30 has a connecting section 31 and a bending section 32. The connecting section 31 is inserted into the supplementing hole 21. The connecting section 31 has a connecting length L3 in the supplementing hole 21. The connecting length L3 is between 5 and 10 cm, so that the supplementing tube 30 can be connected to the supplementing hole 21 and the nutrient solution can flow into the supplementing hole 21. The bending section 32 is exposed in the accommodating space 11 and connected to the connecting section 31. The user can replenish the nutrient solution from the bending section 32, and the nutrient solution flows from the bending section 32 through the connecting section 31 into the supplementing hole 21. The supplementing tube 30 is made of stainless steel.

[0028] like Figures 1 to 3 As shown, in an embodiment of the present invention, when the bacteria 200 have covered the upper half of the porous carrier 20, if the user replenishes the nutrient solution from above, the nutrient solution will be blocked by the bacteria 200 and will not easily penetrate into the interior of the porous carrier 20, and the bacteria 200 will be blocked between the receiving groove 12 and the guide portion 13, making it difficult for water to flow into the receiving groove 12, resulting in restricted growth of the bacteria 200. Thus, the present invention enables the nutrient solution to flow directly from the replenishing tube 30 through the replenishing hole 21 into the porous carrier 20 and diffuse in the porous carrier 20, thereby solving the problem that the nutrient solution cannot be completely absorbed.

[0029] Furthermore, the user can also allow oxygen to be directly injected into the porous carrier 20 from the replenishing pipe 30 through the replenishing hole 21, so as to adjust the carbon dioxide concentration in the porous carrier 20, so that the carbon dioxide concentration is maintained below a standard value, and the bacteria 200 can receive the required oxygen, so as to facilitate the subsequent growth of the bacteria 200. Among them, the standard value is 2000ppm, and more preferably, the standard value is 500ppm, so as to reduce the probability of carbon dioxide causing adverse effects on the bacteria 200.

[0030] In addition, before the user wants to add nutrient solution or inducer, the user can first pour alcohol into the supplement tube 30, so that the alcohol flows into the supplement hole 21 and eliminates the mycelium in the supplement hole 21, so as to prevent the mycelium in the supplement hole 21 from hindering the subsequent supplement of nutrient solution or inducer. Among them, the inducer can promote the growth of the fungus 200, and the inducer can be an edible plant of the Geranium family, such as the essential oil extracted from geranium, the essential oil of citrus plants, the essential oil of Asteraceae plants, etc.

[0031] The fixing plug 40 is sleeved on the periphery of the replenishing pipe 30. Figures 1 to 3 As shown, in an embodiment of the present invention, the fixed plug 40 has a resting portion 41 and a plugging portion 42, the resting portion 41 rests against the side edge of the porous carrier 20; the plugging portion 42 is arranged in the supplementary hole 21, so that the fixed plug 40 can fill the gap between the supplementary hole 21 and the supplementary tube 30, and enable the supplementary tube 30 to be stably inserted into the supplementary hole 21.

[0032] The cover 50 is selectively covered on the box body 10. Figures 1 to 3 As shown, in the embodiment of the present invention, the cover 50 has a through hole 51 and a breathable member 52. The position of the through hole 51 corresponds to the opening of the bent section 32 of the supplementing tube 30, so that the user can directly pour the nutrient solution into the supplementing tube 30 through the through hole 51 without removing the cover 50, thereby improving convenience and preventing the environment in the box 10 from changing too much to avoid affecting the growth of the bacteria 200; the breathable member 52 is selectively covered on the through hole 51, and the breathable member 52 can allow oxygen in the air to flow into the box 10 to provide growth for the bacteria 200. The cover 50 can be a film-shaped protective film, or a cover made of plastic or silicone.

[0033] In this way, the present invention enables the user to pour the nutrient solution into the replenishing tube 30, so that the nutrient solution is directly replenished from the replenishing tube 30 to the porous carrier 20 through the replenishing hole 21 and diffuses in the porous carrier 20, thereby solving the problem that the nutrient solution cannot be completely absorbed by the bacteria 200 and promoting the bacteria 200 to continue to grow.

[0034] Furthermore, the user can also input substances required for the growth of the bacteria 200 (such as oxygen, inducers, etc.) directly into the porous carrier 20 from the replenishment tube 30 through the replenishment hole 21 according to the growth conditions of the bacteria 200, so that the bacteria 200 can grow in the best growth environment.

[0035] Although the present invention is described with a preferred embodiment, those skilled in the art can make various changes without departing from the spirit and scope of the present invention. The above embodiments are only used to illustrate the present invention, not to limit the scope of protection of the present invention. All modifications or changes that do not violate the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A bacterial cell culture device, characterized in that: The bacterial cell culture device comprises: A box body having a containing space; a porous carrier disposed in the accommodating space, the porous carrier having a supplementary hole, the supplementary hole penetrating the porous carrier along a horizontal direction, the length of the supplementary hole along the horizontal direction being shorter than the length of the porous carrier along the horizontal direction; and A supplementary tube has one end inserted into the supplementary hole.

2. The bacterial cell culture device according to claim 1, wherein: The diameter of the supplementary hole is between 14 and 19 mm.

3. The bacterial cell culture device according to claim 1, characterized in that: The ratio of the length of the supplementary hole along the horizontal direction to the length of the porous carrier along the horizontal direction is between 3:5 and 4:

5.

4. The bacterial cell culture device according to claim 1, characterized in that: The supplementing pipe has a connecting section and a bending section. The connecting section is inserted into the supplementing hole, and the bending section is exposed in the accommodating space.

5. The bacterial cell culture device according to claim 4, characterized in that: The connecting section has a connecting length in the supplementary hole, and the connecting length is between 5 and 10 centimeters.

6. The bacterial cell culture device according to claim 1, characterized in that: It also includes a fixed plug, which is sleeved on the periphery of the supplementary pipe.

7. The bacterial cell culture device according to claim 6, characterized in that: The fixed plug has a supporting portion and a plugging portion. The supporting portion is supported against the side edge of the porous carrier, and the plugging portion is arranged in the supplementary hole.

8. The bacterial cell culture device according to claim 1, characterized in that: The accommodating space is concavely provided with an accommodating groove, and the accommodating groove is used for placing the porous carrier. The accommodating space is provided with an inclined guide portion at the periphery of the accommodating groove.

9. The bacterial cell culture device according to claim 1, characterized in that: The box also includes a cover body, which is selectively covered on the box body.

10. The bacterial cell culture device according to claim 9, characterized in that: The cover body has a through hole and a breathable member. The position of the through hole corresponds to the opening of the supplementary tube. The breathable member is selectively covered on the through hole.

Citation Information

Patent Citations

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  • Sampling device for digestive system department examination

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