Anti-aging composition and cosmetic capable of regulating circadian rhythm and retarding social time difference and preparation method of anti-aging composition and cosmetic

Abstract

The invention relates to an anti-aging composition capable of regulating circadian rhythm and retarding social contact time difference, a cosmetic and a preparation method of the anti-aging composition. The anti-aging composition is prepared from hydrolyzed yeast protein wrappage, rice fermentation liquor and L-sodium lactate in a mass ratio of (0.1-5): (0.1-10): (0.1-2). Compared with the prior art, by adjusting internal mechanisms such as biological clock and by simulating or enhancing natural circadian rhythm of the skin, physiological functions of the skin are optimized, so that skin aging is delayed. The skin can be repaired and regenerated at correct time, so that a more lasting anti-aging effect is realized.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an anti-aging composition capable of regulating circadian rhythm and alleviating social jet lag, cosmetics and a preparation method thereof. Background Art

[0002] Social jet lag, as a phenomenon of mismatch between the rhythm of modern life and the human body's biological clock, is essentially the manifestation of circadian rhythm disorder at the individual level. Its roots can be traced back to environmental factors such as the ever-accelerating pace of modern life, extended working hours, frequent social activities at night, and long-term exposure to artificial light sources (especially blue light).

[0003] Circadian rhythm is a 24-hour cycle in the physiological processes of organisms. In humans, it is driven by biological clocks in the brain (suprachiasmatic nucleus) and in each individual cell (clock genes). The biological clock synchronizes and coordinates gene expression, metabolism and behavior at specific times of the day.

[0004] In the skin, circadian rhythm also performs basic functions. Key physiological indicators such as skin temperature, sebum secretion, pH value, transepidermal water loss (TEWL) and capacitance are all finely regulated by circadian rhythm to ensure that the skin remains in optimal condition at different time periods. However, the existence of social jet lag seriously disrupts this natural balance, causing abnormal metabolic activity of skin cells and a significant decrease in self-repair ability, which in turn accelerates the aging process of the skin.

[0005] The skin is the first line of defense between the external environment and the human body. Its various physiological functions (such as skin temperature regulation, sebum secretion, water balance, epidermal cell renewal, etc.) are all regulated by the circadian rhythm. The biological clock disorder caused by social jet lag directly interferes with the normal operation of these physiological functions, which in turn causes a series of skin problems, such as impaired barrier function, increased water loss, slowed cell metabolism and accelerated aging.

[0006] The core of social jet lag lies in the abnormal expression of circadian clock genes. These genes are activated or inhibited during specific time periods to coordinate the physiological functions of various systems throughout the body. In the skin, abnormal expression of circadian clock genes can lead to disorders in cell signal transduction pathways, thereby affecting key processes such as cell proliferation, differentiation and apoptosis.

[0007] Conventional anti-aging approaches on the market often adopt a "one-size-fits-all" strategy, using the same solution for all skin types and problems. However, each person's skin condition, biological clock, and aging mechanism are unique, so this approach may not provide optimal results.

[0008] Patent CN202211494327.X discloses a composition for improving exogenous aging, its preparation method and application. According to weight, the composition comprises: 10-40 parts of hydrangea extract, 10-40 parts of plankton extract, 10-40 parts of desert rose (ADENIUM OBESUM) leaf cell extract, 10-20 parts of European summer solstice grass extract, and 10-20 parts of hydrolyzed yeast protein. Although this technology has the effects of repairing ultraviolet damage, resisting cold and heat aging, anti-oxidation, anti-pollution, regulating the circadian rhythm of the skin, and accurately solving the exogenous aging caused by the environment and bad work and rest in all directions, it has the effects of improving skin moisture, brightening skin color, reducing wrinkles, shrinking pores, and improving skin texture. However, this technology does not consider how to regulate or improve skin aging caused by circadian rhythm disorders (such as social jet lag); it does not provide solutions for these biological clock synchronization mechanisms, and cannot fundamentally solve the skin aging problem caused by social jet lag.

[0009] Conventional anti-aging methods often ignore the important impact of the biological clock on skin aging. The biological clock controls the metabolism, repair and regeneration of the skin, resulting in limited skin care effects. It can only improve the skin condition in the short term, but lacks long-term effects. Therefore, it is urgent to develop an anti-aging product that takes into account the influence of internal factors such as the biological clock from the perspective of social jet lag and circadian rhythm.

[0010] Patent application CN201711007938.6 discloses a composition for preventing or improving circadian rhythm disorders, containing a plant extract of the genus Lespedeza as an effective ingredient, wherein the plants of the genus Lespedeza are selected from one or more plants in the group consisting of Lespedeza, Scoparia truncatula and Lespedeza latifolia, and the circadian rhythm disorder is caused by biological rhythm disorders, which are caused by the reduced expression of biological clock genes. However, the description of its specific formula, preparation process and stability in cosmetics, pharmaceuticals and food compositions is relatively vague, which may affect its effect in practical applications; and it does not take into account the common social jet lag phenomenon in modern life (such as jet lag travel, night shift work, night social activities, etc.) and its specific impact on skin aging, and does not have the effect of directly improving skin aging. Summary of the invention

[0011] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide an anti-aging composition, cosmetics and preparation method thereof that can regulate circadian rhythm and reduce social jet lag.

[0012] The purpose of the present invention can be achieved by the following technical scheme: an anti-aging composition with the function of regulating circadian rhythm and reducing social jet lag, comprising a hydrolyzed yeast protein wrapper, the rice fermentation liquid and the sodium L-lactate in a mass ratio of (0.1-5): (0.1-10): (0.1-2).

[0013] Furthermore, the mass ratio of the hydrolyzed yeast protein coating, the rice fermentation broth and the sodium L-lactate is: (1-5): (1-5): (0.3-2).

[0014] Furthermore, the weight percentage of the hydrolyzed yeast protein in the hydrolyzed yeast protein coating is 2-7%.

[0015] Furthermore, the outer surface of the hydrolyzed yeast protein is coated with a phospholipid bilayer, and the weight percentage of the phospholipid bilayer in the hydrolyzed yeast protein coating is 5-30%.

[0016] Furthermore, the hydrolyzed yeast protein coating is prepared by the following method:

[0017] 1) Dissolve the phospholipids fully in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator to remove the ethanol by low temperature evaporation to form a uniform phospholipid film on the bottom surface of the container; the mass volume ratio of phospholipids to ethanol is (1-5): (5-50) g / mL.

[0018] 2) Stir and mix the phosphate buffer solution and the hydrolyzed yeast protein; the mass volume ratio of the hydrolyzed yeast protein to the phosphate buffer solution is (2000-3000): (20-50) mg / mL

[0019] 3) The mixed solution in step 2) is transferred to a rotary evaporator container to treat the phospholipid film with water (i.e., mixing, stirring and ultrasonic treatment). After the film is completely detached, ultrasonic treatment is performed for 3-5 minutes to obtain a hydrolyzed yeast protein coating with a smaller particle size.

[0020] The lecithin membrane material is selected from white soybean lecithin, egg phospholipids, and cephalin; the particle size of the hydrolyzed yeast protein wrapping material is between 0.1 and 1 micron.

[0021] Furthermore, the rice fermentation liquid contains ketoglutaric acid, and the mass proportion of ketoglutaric acid in the rice fermentation liquid is 300ppm-1000ppm.

[0022] The present invention also provides a cosmetic comprising 0.3 to 17 wt %, more preferably 2.3 to 12 wt % of the anti-aging composition.

[0023] The cosmetics include essence, emulsion, cream, freeze-dried powder or skin care water.

[0024] Furthermore, the cosmetic is an essence, comprising phase A, phase B, phase C, phase D and phase E, each comprising the following components in percentage by weight:

[0025] Phase A includes:

[0026] Polysorbate-20 0.1-20%,

[0027] Carbomer 0.1-15%,

[0028] Sodium hyaluronate 0.01-1%,

[0029] Allantoin 0.1-10%,

[0030] Glycerol 5-20%,

[0031] Butanediol 1-10%,

[0032] Water balance;

[0033] Phase B includes:

[0034] Cetyl alcohol 1-15%,

[0035] Polysorbate-80 0.1-5%,

[0036] Squalane 1-15%;

[0037] Phase C includes: arginine 0.1-5%;

[0038] Phase D includes:

[0039] Anti-aging composition: 0.3-17%;

[0040] Phase E includes:

[0041] (Daily use) essence 0.1-2%;

[0042] Phenoxyethanol 0.1-1%,

[0043] Ethylhexylglycerin 0.05-1%.

[0044] Furthermore, the essence is prepared by the following method:

[0045] a. Water phase: Place water in a container with a stirring device and stir. After fully wetting and mixing the other components of phase A, add them into the water, stir thoroughly until completely dissolved, and heat to 75-80°C;

[0046] b. Oil phase: Mix all components of phase B and heat to 75-80°C, stirring until completely dissolved;

[0047] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes;

[0048] d. Add the components in phase C, stir at 250-350 rpm for 3-5 minutes, then homogenize at 2500-3500 rpm for 3-10 minutes, and cool to 40-50°C with stirring;

[0049] e. Add the components in phase E, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes;

[0050] f. After cooling to 35-38°C, add phase D, stir at 250-350 rpm for 3-10 min, and then homogenize at 2500-3500 rpm for 3-10 minutes to obtain the essence.

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] 1. The present invention selects a specific proportion of hydrolyzed yeast protein wrapping, rice fermentation liquid, and sodium L-lactate for synergistic effect, wherein the hydrolyzed yeast protein wrapping plays an important role during the day, and helps the skin resist free radicals and oxidative stress in the daytime environment through its strong antioxidant ability and repair properties. During the day, the skin is exposed to ultraviolet rays and pollutants, and it can protect the skin from damage and promote the repair of damaged cells; rice fermentation liquid and sodium L-lactate play an important role at night, and the rice fermentation liquid is rich in organic acids and natural peptides, which helps to enhance the skin's moisturizing and repairing ability and promote the recovery of the skin barrier at night. At the same time, sodium L-lactate can regulate the stratum corneum, promote skin renewal and cell metabolism, and make the skin color more even and bright; the antioxidant protection during the day and the repair ability at night are synchronized with the skin's biological clock to maximize the skin care effect.

[0053] 2. The present invention starts from the aspects of social jet lag and circadian rhythm, and formulates precise skin care plans by understanding the individual's biological clock characteristics and skin needs.

[0054] 3. The present invention delays skin aging by regulating internal mechanisms such as the biological clock, simulating or enhancing the skin's natural circadian rhythm, and optimizing its physiological functions, so that the skin can be repaired and regenerated at the right time, thereby achieving a more lasting anti-aging effect. DETAILED DESCRIPTION

[0055] The present invention is described in detail below with reference to specific embodiments.

[0056] The present invention uses a specific ratio of hydrolyzed yeast protein wrap, rice fermentation liquid, and sodium L-lactate to work synergistically, and by adjusting the internal mechanisms such as the biological clock, by simulating or enhancing the natural circadian rhythm of the skin, optimizes its physiological functions, thereby delaying skin aging, allowing the skin to repair and regenerate at the right time, and thus achieving a more lasting anti-aging effect. The specific description is as follows:

[0057] 1. Hydrolyzed yeast protein

[0058] Hydrolyzed yeast protein is a small molecule extracted and converted from yeast using advanced enzymatic technology. It is rich in amino acids, small peptides, B vitamins, glutathione and nucleotides. These ingredients are not only easily absorbed by the skin, but also have a variety of biological activities, providing all-round nourishment and protection for the skin.

[0059] Hydrolyzed yeast protein can significantly enhance the skin's self-protection ability and promote the regeneration and repair of skin cells. Its rich antioxidant substances (such as glutathione) can neutralize free radicals and reduce the damage of the external environment to the skin; while ingredients such as amino acids and small peptides can promote cell metabolism, accelerate the repair and replacement of damaged cells, and keep the skin healthy.

[0060] Another important role of hydrolyzed yeast protein is to help optimize the biological clock of cells, so that the physiological activities of the skin are synchronized with the circadian rhythm. By regulating the expression of biological clock genes in skin cells, hydrolyzed yeast protein can promote the skin to function optimally in different modes during the day and night. During the day, it helps the skin improve its defense and resist external damage such as ultraviolet rays; at night, it promotes repair processes such as DNA repair and skin regeneration, ensuring that the skin gets enough rest and recovery at night.

[0061] In response to the social jet lag problem that is prevalent in modern life, hydrolyzed yeast protein is used to develop an anti-aging formula that can regulate the skin's biological clock and relieve skin stress caused by jet lag.

[0062] Hydrolyzed yeast protein can also be used in combination with other bioactive raw materials to achieve more significant anti-aging effects through the synergistic effect between different ingredients.

[0063] The present invention coats the surface of the hydrolyzed yeast protein with a phospholipid bilayer, the effect of which is that the phospholipid bilayer structure can simulate a biological membrane, enhance the permeability of the hydrolyzed yeast protein on the skin, and protect the active ingredients from damage by the external environment (such as oxygen, light, pH changes), thereby maintaining its biological activity and stability.

[0064] The present invention uses commercially available hydrolyzed yeast protein and adopts the following method to prepare the hydrolyzed yeast protein wrapping:

[0065] 1) Dissolve the phospholipids in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator to remove the ethanol by low-temperature evaporation to form a uniform phospholipid film on the bottom surface of the container; the mass volume ratio of phospholipids to ethanol is (1-5): (5-50) g / mL.

[0066] 2) Stir and mix the phosphate buffer solution and the hydrolyzed yeast protein; the mass volume ratio of the hydrolyzed yeast protein to the phosphate buffer solution is (2000-3000): (20-50) mg / mL.

[0067] 3) The mixed solution in step 2) is transferred to a container of a rotary evaporator to dehydrate the phospholipid film, and after the film is completely detached, ultrasonic treatment is performed for 3-5 minutes to obtain a hydrolyzed yeast protein coating with a smaller particle size.

[0068] The lecithin membrane material is selected from white soybean lecithin, egg phospholipids, and cephalin; the particle size of the hydrolyzed yeast protein wrapping is between 0.1 and 1 micron; the weight percentage of the hydrolyzed yeast protein in the hydrolyzed yeast protein wrapping is 2-7%, and the weight percentage of the phospholipid bilayer in the hydrolyzed yeast protein wrapping is 5-30%.

[0069] 2. Rice fermented liquid

[0070] Rice fermented liquid, as a precision biological fermentation product derived from rice grains, is carefully selected from specific strains in a wide range of yeast libraries and carefully prepared through a highly controlled biological fermentation process. This process not only retains the natural essence of rice grains, but also enriches more than 90 high-purity and high-activity natural skin care ingredients through the metabolic activities of microorganisms, including but not limited to key active compounds such as amino acids, peptides, and organic acids.

[0071] Particularly, the rice fermentation liquid is rich in Alpha-KG (ketoglutaric acid), a natural product of the fermentation process, with a concentration precisely controlled between 300ppm and 1000ppm. As a highly efficient mitochondrial energy metabolism promoter, Alpha-KG not only exhibits excellent antioxidant defense capabilities, but also significantly enhances the energy metabolism efficiency and self-repair mechanism of cells, enhances the skin's resistance to the external environment, regulates sebum secretion, improves skin condition, and makes the skin more fresh, firm and shiny.

[0072] In addition, rice fermented liquid has excellent moisturizing and penetration enhancement effects due to its unique molecular structure and physical and chemical properties. It can optimize the skin barrier function, promote the efficient penetration and absorption of subsequent skin care active substances, ensure that these ingredients can penetrate deep into the skin, accurately act on target cells, and achieve the best skin care effect.

[0073] Although rice fermented liquid does not directly intervene in the regulation mechanism of the biological clock to cope with social jet lag, it indirectly improves the skin rhythm disorder caused by jet lag by deeply nourishing, strengthening skin self-repair and energy metabolism. This endogenous revitalizing effect helps the skin restore its balance and enhance its ability to adapt to changes in the external environment, thus showing an effective mitigation effect on the challenge of social jet lag at the macro level.

[0074] The rice fermentation liquid used in the present invention can be a commercially available product, or it can be prepared by the method reported in the existing literature. The rice fermentation liquid used in the following embodiments is prepared by the method disclosed in CN202211506070.5.

[0075] 3. Sodium L-lactate

[0076] Sodium L-lactate is produced by fermentation of sugar and has significant antibacterial activity. As a new generation of skin whitening agent, it has a whitening effect itself, and when used in combination with other whitening agents, it exhibits synergistic properties, further enhancing the skin whitening effect. In the regulation of melanin synthesis, tyrosinase is a key catalytic enzyme. Sodium L-lactate can effectively inhibit its activity, thereby reducing the formation of melanin, helping to achieve uniform skin tone and reduce age spots.

[0077] In addition, as the sodium salt form of L-lactic acid, sodium L-lactate has a slight exfoliating effect, promoting the natural metabolism and renewal of the stratum corneum, making the skin texture smoother and more uniform, reducing epidermal pigmentation and improving uneven skin color.

[0078] And at low concentrations, sodium L-lactate can help maintain the skin's natural acid-base balance, which is very important for protecting the skin barrier and reducing bacterial infections. Healthy skin pH is usually acidic, and sodium L-lactate can help maintain this acidic environment and improve the skin's resistance to the external environment.

[0079] The L-sodium lactate used in the present invention is a commercially available product.

[0080] 4. Synergy

[0081] The present invention adopts a hydrolyzed yeast protein package, rice fermentation liquid and sodium L-lactate in a specific mass ratio of (0.1-5): (0.1-10): (0.1-2) to work synergistically. The hydrolyzed yeast protein package plays an important role during the day, and helps the skin resist free radicals and oxidative stress in the daytime environment through its strong antioxidant ability and repair properties. During the day, the skin is exposed to ultraviolet rays and pollutants, which can protect the skin from damage and promote the repair of damaged cells; rice fermentation liquid and sodium L-lactate play an important role at night. The rice fermentation liquid is rich in organic acids and natural peptides, which helps to strengthen the skin's moisturizing and repairing ability and promote the recovery of the skin barrier at night. At the same time, sodium L-lactate can regulate the stratum corneum, promote skin renewal and cell metabolism, and make the skin color more uniform and bright; the antioxidant protection during the day and the repair ability at night are synchronized with the skin's biological clock to maximize the skin care effect.

[0082] The present invention uses essence as an example to set up each group of experiments, but is not limited to the use of essence. The composition can also be applied to various skin care products such as lotions, creams, freeze-dried powders, and skin care water. The essence includes the above key composition and a skin care agent, a thickener, a skin conditioner, and a pH regulator. Preferably, the raw materials for preparing the anti-aging essence that can regulate circadian rhythms and reduce social jet lag also include any one of preservatives and flavors, or a combination of at least two of them.

[0083] The essence formula also includes phase A auxiliary material, phase B auxiliary material, phase C auxiliary material and phase E auxiliary material, based on 100 parts by weight of the essence, specifically:

[0084] The phase A auxiliary material includes 0.1 to 30 parts of thickener A, 0.1 to 30 parts of skin conditioner A and 1 to 30 parts of moisturizer A;

[0085] The phase B auxiliary material includes 0.1 to 30 parts of thickener B, 0.05 to 30 parts of emulsifier and 2 to 15 parts of excipient;

[0086] The phase C auxiliary material includes 0.1 to 5 parts of a pH adjuster;

[0087] The E phase auxiliary material includes 0.1 to 5 parts of a fragrance, 0.01 to 1 part of a preservative, and 0.01 to 5 parts of a moisturizer E;

[0088] Optionally, the phase A auxiliary material further includes water, and the amount of water used is such that the weight parts of the essence reaches 100 parts.

[0089] The thickener A and the thickener B each independently include one or more of polyacrylamide, polysorbate-20, xanthan gum, propylene glycol, behenyl alcohol, carbomer, myristyl alcohol, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose;

[0090] The skin conditioning agent A and the skin conditioning agent B each independently include one or more of glycerin, bisabolol, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate;

[0091] The moisturizer A and moisturizer E independently include one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, ethylhexylglycerin, hydrogenated grape seed oil and shea butter;

[0092] The emulsifier includes one or more of cetearyl glucoside (and) cetearyl alcohol, polysorbate-80, polysorbate-20, cocoglycerides and sorbitan olivate;

[0093] The pH regulator includes one or more of arginine and lactic acid;

[0094] The aromatic agent includes essence;

[0095] The preservative includes one or more of phenoxyethanol, ethylhexylglycerin and pentylene glycol.

[0096] The formulas of the essences used in the following examples are shown in Table 1:

[0097] Table 1 Essence formula

[0098]

[0099] The hydrolyzed yeast protein coating in the above table is prepared by the following method:

[0100] 1500 mg of lecithin is dissolved in 15 mL of ethanol, stirred until fully dissolved, and then transferred to a rotary evaporator. In a water bath at 65° C., the ethanol is slowly evaporated to form a uniform lecithin film on the container wall. 30 mL of phosphate buffer solution and 2500 mg of hydrolyzed yeast protein are stirred and mixed, and the mixed solution is transferred to a container of a rotary evaporator to hydrate the lecithin film for 20 minutes. After the film is completely detached, ultrasonic treatment is performed for 3 minutes to obtain hydrolyzed yeast protein functional liposomes; the particle size of the liposomes is 0.1-1 microns.

[0101] The essence is prepared by the following method:

[0102] a. Water phase: Place water in a container with a stirring device and stir. After fully wetting and mixing the other components of phase A, add them into the water, stir thoroughly until completely dissolved, and heat to 75-80°C;

[0103] b. Oil phase: Mix all components of phase B and heat to 75-80°C, stirring until completely dissolved;

[0104] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes;

[0105] d. Add the components in phase C, stir at 250-350 rpm for 3-5 minutes, then homogenize at 2500-3500 rpm for 3-10 minutes, and cool to 40-50°C with stirring;

[0106] e. Add the components in phase E, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes;

[0107] f. After cooling to 35-38°C, add phase D, stir at 250-350 rpm for 3-10 minutes, and then homogenize at 2500-3500 rpm for 3-10 minutes to obtain the essence. Filter and sterilize before filling, and test the bacterial content, toxins, immunogenicity, etc. for quality control.

[0108] All the above steps were strictly controlled for sterility.

[0109] The following is a detailed description through specific examples. The raw materials and detection methods involved in the present invention are commonly used raw materials and detection methods in the art unless otherwise specified.

[0110] Examples 1-3 and Comparative Examples 1-7

[0111] The essences of Examples 1-3 and Comparative Examples 1-7 were prepared using the formula described in Table 2 below and the above method.

[0112] Table 2 Formulations of Examples 1-3 and Comparative Examples 1-7

[0113]

[0114]

[0115] The difference between Example 2 and Example 1 is only the increase of each effective ingredient, and the difference between Example 3 and Example 1 is only the reduction of each effective ingredient.

[0116] The difference between Comparative Examples 1-3 and Example 1 is only the lack of one key composition component, the difference between Comparative Examples 4-6 and Example 1 is only the lack of two key composition components, and the difference between Comparative Example 7 and Example 1 is only the lack of all key composition components.

[0117] Effect test

[0118] (I) Test on the effect of promoting skin regeneration and repair

[0119] According to the formula in Table 3 below, the hydrolyzed yeast protein coating, rice fermentation liquid and sodium L-lactate were evenly mixed with water to prepare the experimental sample.

[0120] Table 3 Weight distribution ratio of raw material components in cell ROS inhibition experiment (wt%)

[0121]

[0122] Cell ROS inhibition experiment, the specific steps are as follows:

[0123] (1) Cell seeding: Human keratinocytes HaCat were seeded at 2-3×10 5 The cells were inoculated into the wells of a 24-well culture plate at a density of 1.5 cells / well and then placed in a CO2 cell culture incubator for 24 hours.

[0124] (2) According to the experimental grouping, the test substance was added to the cells inoculated in step (1) and cultured for 24 hours. The experimental groups were as follows: blank control group (medium without serum, no UVB irradiation), injury model group (medium without serum, UVB irradiation), positive control group (vitamin C 200 ug / mL), experimental examples 1-3 and blank examples 1-6 (the experimental samples prepared in experimental examples 1-3 and blank examples 1-6 were added to serum-free culture medium to prepare a sample solution of 200 ug / mL).

[0125] (3) Except for the blank control group, the remaining cells were irradiated with UVB (2 J / cm 2 ).

[0126] (4) The culture medium was discarded, the cells were washed and fixed, and the working concentration of the reactive oxygen species fluorescent probe DCFH-DA reagent was added to the cells for co-incubation according to the reagent operating instructions.

[0127] (5) After incubation, the cells were photographed under an inverted fluorescence microscope and the fluorescence intensity was counted.

[0128] The results showed that after UVB irradiation, the intracellular fluorescence intensity increased significantly. Compared with the blank control group, the DCF fluorescence intensity of the damage model group increased to 235.26%, indicating that the UVB light damage model was successfully established; the positive control vitamin C and experimental examples 1-3 were added in advance, and then UVB irradiation was performed. The results showed that vitamin C had a significant effect on reducing intracellular fluorescence, and the fluorescence was almost invisible. After adding experimental examples 1-3, the fluorescence intensity decreased by 133.68%, 174.52%, and 98.23%, respectively, indicating that experimental examples 1-3 all had strong effects on repairing light damage, and the repair effect was proportional to the content of hydrolyzed yeast protein wrapping, rice fermentation broth and L-sodium lactate. After adding blank examples 1-6, the fluorescence intensity decreased by 63.41%, 70.82%, 76.25%, 20.65%, 51.38%, and 47.79%, respectively.

[0129] The experimental fluorescence intensity reduction results are: Experimental Examples 1-3>Blank Examples 1-3>Blank Examples 4-6, indicating that Experimental Examples 1-3 all have strong effects on repairing light damage, and the repair effect is proportional to the content of hydrolyzed yeast protein wrapping, rice fermentation broth and L-sodium lactate. Blank Examples 1-6 contain one or two of the three effective ingredients, and their fluorescence intensity reduction values ​​are lower than those of the experimental examples, indicating that the hydrolyzed yeast protein wrapping, rice fermentation broth and sodium L-lactate have a synergistic effect and can effectively enhance the light damage repair effect.

[0130] The culture medium refers to DMEM culture medium:

[0131] DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture basal medium suitable for the growth of a variety of mammalian cells. It provides the basic nutrients and inorganic salts required for cell growth.

[0132] Serum is commonly used in cell culture to provide growth factors, hormones, transferrin, and other factors that are beneficial to cell growth.

[0133] (II) Safety testing

[0134] 1) Test samples: Examples 1-3 and Comparative Examples 1-7.

[0135] 2) Number of participants: 110 participants, half male and half female, aged between 20 and 55 years old, all meeting the criteria for subjects.

[0136] 3) Test method: 110 subjects who met the test criteria were randomly divided into 11 groups, corresponding to the essences obtained in Examples 1-3 and Comparative Examples 1-7 described as test subjects, and blank group 1 was a group that did not apply any sample, with 10 subjects in each group; a third-party testing agency was used to test safety. The selected area should not exceed 50mm 2 , a suitable patch with a depth of about 1mm, add about 0.02-0.025ml (g) of the test substance into the patch tester by closed patch test method. Stick the patch tester on the curved side of the subject's forearm, remove the test substance after 24 hours, and observe the skin reaction at 0.5h, 24h, and 48h after removal: record the results according to the skin reaction grading standards in the "Cosmetic Safety Technical Regulations 2015".

[0137] Table 3 Patch test results

[0138]

[0139] Note: Skin reaction grading standards:

[0140] Grade 0: negative reaction;

[0141] Grade 1: Suspicious reaction; only slight erythema;

[0142] Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0143] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes: the reaction may extend beyond the tested area;

[0144] Grade 4: Very strong reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0145] 3) Test results: The results in Table 3 show no irritation and are considered safe.

[0146] (III) Stability test

[0147] 1) Test samples: Examples 1-3.

[0148] 2) Test method: Internal control standard, observe the appearance color, viscosity and pH for three months.

[0149] Table 4 Stability test results

[0150] time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE illumination 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0151] Among them, W stands for week, M stands for month, and "CYCLE" refers to cycle. The cycle setting is: heating from -15℃ to 45℃, and cooling from 45℃ to -15℃ for five times.

[0152] 3) Test results: The results in Table 4 show that the essences in Examples 1-3 are stable in properties within 3 months.

[0153] (IV) Evaluation of anti-aging effects

[0154] 1) Test samples: Examples 1-3, Comparative Examples 1-7.

[0155] 2) Test subjects: 100 healthy women (23-45 years old, average age 34.6 years old) were randomly divided into 10 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, a series of questions about medical history and health status were asked according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0156] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0157] 3) Test method: Use the sample twice a day, morning and evening, for 13 weeks. Apply an appropriate amount of the test sample around one eye of the subject, and do not use the test sample around the other eye. Apply sunscreen products to the entire face every morning.

[0158] The test results were collected at the following four time periods: before using the sample (D0), 7 days after using the sample (D7), 14 days after using the sample (D14), and 91 days after using the sample (D91).

[0159] 4) Test items are:

[0160] ①Primos is used for facial image acquisition;

[0161] ②Corneometer CM825 is used to collect the moisture content of the stratum corneum;

[0162] ③ MPA580 is used for skin tightening collection.

[0163] 5) The test results can be calculated using the following formula:

[0164] Change rate = (average analysis value of 10 people in each group after using the product - average analysis value of 10 people in each group before using the product) ÷ average analysis value of 10 people in each group before using the product × 100%

[0165] 6) Test results

[0166] ① Skin wrinkles (outer corners of eyes)

[0167] Primos CR was used for facial image acquisition, skin wrinkle analysis and photo analysis, and the data can be found in Table 5. A decrease in the analysis value indicates that skin wrinkles have improved.

[0168] Table 5 Skin wrinkle Ra value change rate

[0169]

[0170]

[0171] From the results in Table 5, it can be seen that after using the essence, as the use time increases, the change rate of the skin wrinkle Ra value of Examples 1-3 and Comparative Examples 1-6 decreases, indicating that the skin wrinkles have been improved. However, compared with Comparative Examples 1-6, when used for the same time, the change rate of the skin wrinkle Ra value of Example 1-3 is greater, indicating that the improvement effect of the skin wrinkles after using the essence of Examples 1-3 is more significant. There is no obvious improvement in the skin wrinkles of Comparative Example 7.

[0172] From the comparison of the results of Examples 1-3 and Comparative Examples 1-7, it can be seen that when the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate are used at the same time, the improvement effect of skin wrinkles is better, compared with not using the anti-aging composition that regulates circadian rhythm and slows down social jet lag, or only using one or two of the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate. This shows that the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate have a synergistic effect, can effectively improve skin wrinkles, and have an anti-aging effect.

[0173] ②Test results of moisture content of stratum corneum (Corneometer CM825)

[0174] The higher the measurement value, the higher the water content of the skin's stratum corneum.

[0175] Table 6 Change rate of moisture content in stratum corneum

[0176]

[0177]

[0178] As shown in Table 6, after using the essence, as the use time increases, the change rate of the water content of the stratum corneum of Examples 1-3 and Comparative Examples 1-6 increases, indicating that the water content of the stratum corneum of the skin has improved. However, compared with Comparative Examples 1-6, when used for the same time, the change rate of the water content of the stratum corneum of Example 1-3 is greater, indicating that the improvement effect of the water content of the stratum corneum of the skin after using the essence of Example 1-3 is more significant.

[0179] From the comparison of the results of Examples 1-3 and Comparative Examples 1-7, it can be seen that when the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate are used at the same time, the improvement effect of the water content of the stratum corneum of the skin is better than that of not using the anti-aging composition that regulates circadian rhythm and slows down social jet lag, or only using one or two of the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate. This shows that the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate have a synergistic effect, can effectively improve the water content of the stratum corneum of the skin, and have an anti-aging effect.

[0180] ③ Skin firming The larger the MPA580) (cheek) measurement, the better the skin firmness.

[0181] Table 7 Skin firmness change rate

[0182]

[0183]

[0184] From the results in Table 7, it can be seen that after using the essence, as the use time increases, the skin firmness change rate of Examples 1-3 and Comparative Examples 1-6 increases, indicating that the skin firmness has improved. However, compared with Comparative Examples 1-6, when used for the same time, the skin firmness change rate of Example 1-3 is greater, indicating that the improvement effect of skin firmness after using the essence of Example 1-3 is more significant.

[0185] From the comparison of the results of Examples 1-3 and Comparative Examples 1-7, it can be seen that when the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate are used at the same time, the improvement effect of skin firmness is better than that of not using the anti-aging composition that regulates circadian rhythm and slows down social jet lag, or using only one or two of the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate. This shows that the hydrolyzed yeast protein wrap, rice fermentation broth, and sodium L-lactate have a synergistic effect, can effectively improve skin firmness, and have an anti-aging effect.

[0186] In summary, the composition provided by the present invention can effectively improve skin wrinkles, improve the water content of the skin stratum corneum, improve skin firmness, and thus delay skin aging by regulating internal mechanisms such as the biological clock, simulating or enhancing the natural circadian rhythm of the skin, and optimizing its physiological functions.

[0187] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. An anti-aging composition capable of regulating circadian rhythm and alleviating social jet lag, characterized in that: The invention comprises a hydrolyzed yeast protein wrapping material, the rice fermentation liquid and the sodium L-lactate in a mass ratio of (0.1-5):(0.1-10):(0.1-2).

2. The anti-aging composition according to claim 1, which has the function of regulating circadian rhythm and reducing social jet lag, is characterized in that: The mass ratio of the hydrolyzed yeast protein wrapping, the rice fermentation liquid and the sodium L-lactate is: (1-5): (1-5): (0.3-2).

3. The anti-aging composition for regulating circadian rhythm and alleviating social jet lag according to claim 1, characterized in that: The weight percentage of the hydrolyzed yeast protein in the hydrolyzed yeast protein wrapping is 2-7%.

4. The anti-aging composition for regulating circadian rhythm and alleviating social jet lag according to claim 3, characterized in that: The outer surface of the hydrolyzed yeast protein is coated with a phospholipid bilayer, and the weight percentage of the phospholipid bilayer in the hydrolyzed yeast protein wrapping is 5-30%.

5. The anti-aging composition for regulating circadian rhythm and alleviating social jet lag according to claim 3, characterized in that: The hydrolyzed yeast protein wrap is prepared by the following method: 1) Dissolve the phospholipids in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator to remove the ethanol by low-temperature evaporation to form a uniform phospholipid film on the bottom surface of the container; 2) Mixing the phosphate buffer solution and the hydrolyzed yeast protein and stirring to mix well; 3) The mixed solution obtained in step 2) is transferred to the container of the rotary evaporator in step 1) to treat the phospholipid film with water. After the film is completely detached, ultrasonic treatment is performed for 3-5 minutes to obtain a hydrolyzed yeast protein coating.

6. The anti-aging composition for regulating circadian rhythm and alleviating social jet lag according to claim 1, characterized in that: The rice fermentation liquid contains ketoglutaric acid, and the mass proportion of ketoglutaric acid in the rice fermentation liquid is 300ppm-1000ppm.

7. A cosmetic, characterized in that: Contains 0.3-17 wt % of the anti-aging composition according to any one of claims 1-6.

8. The cosmetic according to claim 7, characterized in that: Including essence, lotion, cream, freeze-dried powder or skin lotion.

9. The cosmetic according to claim 8, characterized in that: The cosmetic is an essence, including phase A, phase B, phase C, phase D and phase E, each of which includes the following components in percentage by weight: Phase A includes: Polysorbate-20 0.1-20%, Carbomer 0.1-15%, Sodium hyaluronate 0.01-1%, Allantoin 0.1-10%, Glycerol 5-20%, Butanediol 1-10%, Water balance; Phase B includes: Cetyl alcohol 1-15%, Polysorbate-80 0.1-5%, Squalane 1-15%; Phase C includes: arginine 0.1-5%; Phase D includes: Anti-aging composition: 0.3-17%; Phase E includes: (Daily use) essence 0.1-2%; Phenoxyethanol 0.1-1%, Ethylhexylglycerin 0.05-1%.

10. The cosmetic according to claim 9, characterized in that: The essence is prepared by the following method: a. Water phase: Place water in a container with a stirring device and stir. After fully wetting and mixing the other components of phase A, add them into the water, stir thoroughly until completely dissolved, and heat to 75-80°C; b. Oil phase: Mix all components of phase B and heat to 75-80°C, stirring until completely dissolved; c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes; d. Add the components in phase C, stir at 250-350 rpm for 3-5 minutes, then homogenize at 2500-3500 rpm for 3-10 minutes, and cool to 40-50°C with stirring; e. Add the components in phase E, stir at 250-350 rpm for 3-5 min, and then homogenize at 2500-3500 rpm for 3-10 minutes; f. After cooling to 35-38°C, add phase D, stir at 250-350 rpm for 3-10 min, and then homogenize at 2500-3500 rpm for 3-10 minutes to obtain the essence.

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