Application of peach gum extract in preparation of product with effect of promoting skin compactness

Abstract

The invention discloses an application of a peach gum extract in preparation of a product with an effect of promoting skin compactness. The preparation method of the peach gum extract comprises the following steps: (1) taking peach gum, carrying out heating reflux extraction with water, filtering after the extraction is finished, and taking filtrate to obtain a peach gum extracting solution; (2) taking natural plants, carrying out heating reflux extraction with water, filtering after the extraction is finished, and taking filtrate to obtain a natural plant extracting solution; and (3) mixing the peach gum extracting solution with the natural plant extracting solution, concentrating under reduced pressure, and freeze-drying to obtain the peach gum extract, in addition, other active ingredients such as schizophyllan can be added in the step (3). Research shows that the peach gum extract prepared by the method disclosed by the invention has a relatively good effect of increasing the collagen content in skin; therefore, further application of the compound serving as an active ingredient to preparation of drugs or skin care products with the effect of promoting skin tightening also has important application value.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to application of a peach gum extract in preparing a product having a skin tightening effect. Background Art

[0002] As we age, skin metabolism slows down and the collagen content in the skin decreases, causing the skin to lack elasticity and firmness. Therefore, increasing the collagen content in the skin is important for promoting skin firmness and reducing skin sagging.

[0003] Peach gum is a reddish brown or yellowish brown gum secreted from the bark of Prunus persica (L.) Batsch or Prunus davidiana (Carr.) Franch, a plant of the Rosaceae family. It is a traditional Chinese medicine, commonly used for blood clots, stranguria with stones, dysentery, abdominal pain, diabetes, and chyluria. However, there is little research on the effective ingredients in peach gum that have the effect of increasing the collagen content in the skin.

[0004] Therefore, it is of great application value to develop a peach gum extract that can increase the collagen content in the skin using peach gum as the main raw material. Summary of the invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention first provides an application of a peach gum extract in preparing a product having a skin tightening effect.

[0006] The above technical problems to be solved by the present invention are achieved through the following technical solutions: The present invention first provides a method for preparing a peach gum extract, which comprises the following steps: (1) taking peach gum, heating and refluxing extraction with water, filtering after the extraction, taking the filtrate, and obtaining peach gum extract; (2) taking a natural plant, heating and refluxing it with water for extraction, filtering it after the extraction is completed, and taking the filtrate to obtain a natural plant extract; (3) The peach gum extract and the natural plant extract are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract.

[0007] The inventors found in their research that the peach gum extract prepared by adding natural plant extract to peach gum extract has a good effect of increasing the collagen content in the skin; its effect of increasing the collagen content in the skin is significantly higher than the peach gum extract prepared by using a simple peach gum extract.

[0008] Preferably, in step (1), the mass ratio of peach gum to water is 1:10-20.

[0009] Most preferably, the mass ratio of peach gum to water in step (1) is 1:15.

[0010] Preferably, the extraction temperature of the heating reflux extraction in step (1) is 80-100° C., and the extraction time is 1-2 h.

[0011] Most preferably, the extraction temperature of the heating reflux extraction in step (1) is 100° C. and the extraction time is 1.5 h.

[0012] Preferably, the natural plants described in step (2) are selected from ginseng and / or angelica dahurica.

[0013] Preferably, the natural plants in step (2) are composed of ginseng and angelica dahurica.

[0014] The ginseng is the dried root of the Araliaceae plant Panax ginseng.

[0015] The described angelica root is the dried root of angelica dahurica of the Umbelliferae family.

[0016] The inventors surprisingly found in further research that the effect of adding peach gum extract prepared by natural plant extracts prepared by using ginseng and angelica as raw materials to peach gum extract in increasing the collagen content in the skin is much higher than that of adding peach gum extract prepared by natural plant extracts prepared by using only ginseng or only angelica as raw materials to peach gum extract. Adding peach gum extract prepared by natural plant extracts prepared by using both ginseng and angelica as raw materials to peach gum extract can synergistically increase the collagen content in the skin.

[0017] More preferably, the mass ratio of ginseng to angelica dahurica is 1~2:1~2.

[0018] Most preferably, the mass ratio of ginseng to angelica dahurica is 1:1.

[0019] Preferably, in step (2), the mass ratio of natural plants to water is 1:10-20.

[0020] Most preferably, the mass ratio of natural plants to water in step (2) is 1:15.

[0021] Preferably, the extraction temperature of the heating reflux extraction in step (2) is 80-100° C., and the extraction time is 1-2 h.

[0022] Most preferably, the extraction temperature of the heating reflux extraction in step (2) is 100° C. and the extraction time is 2 h.

[0023] Preferably, in step (3), the volume ratio of peach gum extract to natural plant extract is 2-4:1.

[0024] Most preferably, the volume ratio of peach gum extract to natural plant extract in step (3) is 3:1.

[0025] Preferably, in step (2), the natural plant extract is further subjected to macroporous resin column chromatography.

[0026] Preferably, the specific method of the macroporous resin column chromatography is: The natural plant extract was loaded onto a macroporous resin column and then diluted with 11-14% ethanol by volume. The impurities are removed by eluting with aqueous solution; then, the mixture is eluted with ethanol aqueous solution with a volume fraction of 27-30%, and the eluate from the ethanol aqueous solution with a volume fraction of 27-30% is collected to obtain the natural plant macroporous resin eluate.

[0027] Most preferably, the specific method of the macroporous resin column chromatography is: The natural plant extract was loaded onto a macroporous resin column and then washed with 12% ethanol water. The impurities are removed by eluting with the solution; then, the mixture is eluted with an ethanol aqueous solution having a volume fraction of 29%, and the eluate from the ethanol aqueous solution having a volume fraction of 29% is collected to obtain the natural plant macroporous resin eluate.

[0028] The inventors found in further research that the effect of increasing the collagen content in the skin was further significantly improved in the peach gum extract prepared by adding natural plant macroporous resin eluate obtained by further macroporous resin treatment of natural plant extract to the peach gum extract, compared with the peach gum extract prepared by simply adding natural plant extract to the peach gum extract.

[0029] The inventors also found in the research that the macroporous resin elution conditions of the present invention are very critical. In the peach gum extract, a natural plant macroporous resin eluent obtained by eluting under the macroporous resin elution conditions of the present invention must be further added, and its effect of increasing the collagen content in the skin can be further significantly improved; however, in the peach gum extract, adding a natural plant macroporous resin eluent obtained by eluting under other macroporous resin elution conditions, its effect of increasing the collagen content in the skin cannot be further significantly improved.

[0030] Preferably, in step (3), the peach gum extract is mixed with the natural plant macroporous resin eluate, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract.

[0031] Preferably, in step (3), the volume ratio of the peach gum extract to the natural plant macroporous resin eluent is 2-4:1.

[0032] Most preferably, in step (3), the volume ratio of the peach gum extract to the natural plant macroporous resin eluent is 3:1.

[0033] Preferably, in step (3), other active ingredients are also added; Specifically, the peach gum extract is mixed with the natural plant extract and other active ingredients, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract.

[0034] Preferably, the other active ingredient is Schizophyllan.

[0035] The invention also provides a peach gum extract prepared by the preparation method.

[0036] The present invention also provides an application of the peach gum extract in preparing a product having the function of increasing the collagen content in the skin.

[0037] The present invention also provides an application of the peach gum extract in preparing a product having the effect of promoting skin firming.

[0038] Preferably, the product is a medicine or a skin care product.

[0039] Beneficial effects: The present invention provides a new method for preparing peach gum extract. Studies have shown that the peach gum extract prepared by the method of the present invention has a good effect of increasing the collagen content in the skin; therefore, it has important application value to use it as an active ingredient to prepare medicines or skin care products that have the effect of increasing the collagen content in the skin. It also has important application value to use it as an active ingredient to prepare medicines or skin care products that promote skin firming. DETAILED DESCRIPTION

[0040] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100°C and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; the natural plant is ginseng; (3) The peach gum extract and the natural plant extract are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant extract is 3:1.

[0042] Example 2 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; the natural plant is Angelica dahurica; (3) The peach gum extract and the natural plant extract are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant extract is 3:1.

[0043] Example 3 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:1; (3) The peach gum extract and the natural plant extract are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant extract is 3:1.

[0044] Example 4 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:1; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 12% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 29% ethanol aqueous solution, and the eluate with the 29% ethanol aqueous solution was collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract and the natural plant macroporous resin eluate are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant macroporous resin eluate is 3:1.

[0045] Example 5 Preparation of peach gum extract (1) Take peach gum, heat it with water to 80°C and perform heating reflux extraction for 2 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:10; (2) taking a natural plant, heating it with water to 80°C and performing heating reflux extraction for 3 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:10; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 2:1; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 11% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 27% ethanol aqueous solution, and the eluate with the 27% ethanol aqueous solution was collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract and the natural plant macroporous resin eluent are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant macroporous resin eluent is 2:1.

[0046] Example 6 Preparation of peach gum extract (1) Take peach gum, heat it with water to 90°C and perform heating reflux extraction for 1 hour. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:20; (2) taking a natural plant, heating it with water to 90° C. and performing heating reflux extraction for 1 hour, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:20; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:2; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 14% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 30% ethanol aqueous solution, and the eluate with 27% ethanol aqueous solution was collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract and the natural plant macroporous resin eluate are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant macroporous resin eluate is 4:1.

[0047] Example 7 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:1; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 12% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 29% ethanol aqueous solution, and the eluate with the 29% ethanol aqueous solution was collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract, the natural plant macroporous resin eluate and the schizophyllan are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the amount ratio of the peach gum extract to the natural plant macroporous resin eluate and the schizophyllan is 100 mL: 40 mL: 10 g.

[0048] Comparative Example 1 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) The peach gum extract is concentrated under reduced pressure and then freeze-dried to obtain the peach gum extract.

[0049] Comparative Example 2 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:1; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 5% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 15% ethanol aqueous solution, and the eluate with a volume fraction of 15% ethanol aqueous solution is collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract and the natural plant macroporous resin eluate are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant macroporous resin eluate is 3:1.

[0050] Comparative Example 3 Preparation of peach gum extract (1) Take peach gum, heat it with water to 100°C and perform heating reflux extraction for 1.5 hours. After the extraction is completed, filter and take the filtrate to obtain peach gum extract; wherein the mass ratio of peach gum to water is 1:15; (2) taking a natural plant, heating it with water to 100° C. and performing heating reflux extraction for 2 hours, filtering after the extraction, taking the filtrate, and obtaining a natural plant extract; wherein the mass ratio of the natural plant to water is 1:15; and the natural plant is composed of ginseng and angelica dahurica in a mass ratio of 1:1; Further, the natural plant extract is placed on a D101 macroporous resin column (the column volume of the macroporous resin column is the same as the volume of the natural plant extract), and then eluted with 1 column volume of 25% ethanol aqueous solution to remove impurities; then eluted with 1 column volume of 50% ethanol aqueous solution, and the eluate with 50% ethanol aqueous solution was collected to obtain a natural plant macroporous resin eluate; (3) The peach gum extract and the natural plant macroporous resin eluate are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract; wherein the volume ratio of the peach gum extract to the natural plant macroporous resin eluate is 3:1.

[0051] Experimental Example 1 Human dermal fibroblasts HSF (SV40) in the logarithmic growth phase were cultured at 1 x 10 5The concentration of 1 μg / well was added to a 96-well plate containing DMEM high-glucose cell culture medium; after human dermal fibroblasts HSF (SV40) were completely attached to the wall, 20 μL of the sample solution with a concentration of 50 μg / mL was added to each well, and after continuing to culture for 24 hours, the culture medium was removed; after washing with PBS buffer, 1 μL of PMSF protease inhibitor and 100 μL of RIPA lysis buffer (Nanjing Senbeijia Biotechnology Co., Ltd.) were added to each well, and lysed at 4°C for 25 minutes; then centrifuged at 4°C and the supernatant was taken; then the human type I collagen (COL-1) ELISA kit was used to detect the content of type I collagen in the supernatant.

[0052] Experimental groups 1 to 7 were set up according to the above method, and a blank control group without the test sample was set up; 3 replicate wells were set up in each group and the test was repeated 3 times; the increase rate of type I collagen content in each experimental group relative to the blank control group was calculated.

[0053] The increase rate of type I collagen content (%) = [(type I collagen content in the experimental group - collagen content in the blank control group) / type I collagen content in the experimental group] x 100%.

[0054]

[0055] It can be seen from the experimental results in Table 1 that the increase rate of type I collagen content in the peach gum extracts prepared in Examples 1 and 2 is significantly higher than that in the peach gum extract prepared in Comparative Example 1; this indicates that the peach gum extract prepared by adding natural plant extracts to peach gum extracts has a good effect of increasing the collagen content in the skin; and its effect of increasing the collagen content in the skin is significantly higher than that of the peach gum extract prepared by using a simple peach gum extract.

[0056] It can be seen from the experimental results in Table 1 that the increase rate of type I collagen content in the peach gum extract prepared in Example 3 is much higher than that in the peach gum extract prepared in Examples 1 and 2; this shows that the effect of adding the peach gum extract prepared by the natural plant extract prepared by using ginseng and angelica as raw materials to the peach gum extract to increase the collagen content in the skin is much higher than that of adding the peach gum extract prepared by the natural plant extract prepared by using only ginseng or only angelica as raw materials to the peach gum extract. Adding the peach gum extract prepared by the natural plant extract prepared by using both ginseng and angelica as raw materials to the peach gum extract can synergistically increase the collagen content in the skin.

[0057] It can be seen from the experimental results in Table 1 that the increase rate of type I collagen content in the peach gum extract prepared in Example 4 is further significantly higher than that in the peach gum extract prepared in Example 3; this indicates that the effect of increasing the collagen content in the skin by adding the natural plant macroporous resin eluate obtained by further macroporous resin treatment of the natural plant extract to the peach gum extract is further significantly improved compared to the peach gum extract prepared by simply adding the natural plant extract to the peach gum extract.

[0058] It can be seen from the experimental results in Table 1 that the increase rate of type I collagen content in the peach gum extracts prepared in Comparative Examples 2 and 3 is higher than that in the peach gum extract prepared in Example 3, but the increase is not large and is much smaller than that in the peach gum extract prepared in Example 4. This shows that the macroporous resin elution conditions of the present invention are very critical. In the peach gum extract, the natural plant macroporous resin eluate obtained by eluting under the macroporous resin elution conditions of the present invention must be further added to the peach gum extract, and its effect of increasing the collagen content in the skin can be further greatly improved; however, in the peach gum extract, the natural plant macroporous resin eluate obtained by eluting under other macroporous resin elution conditions is added, and its effect of increasing the collagen content in the skin cannot be further greatly improved.

Claims

1. A method for preparing peach gum extract, characterized in that: The following steps are included: (1) taking peach gum, heating and refluxing extraction with water, filtering after the extraction, taking the filtrate, and obtaining peach gum extract; (2) taking a natural plant, heating and refluxing it with water for extraction, filtering it after the extraction is completed, and taking the filtrate to obtain a natural plant extract; (3) The peach gum extract and the natural plant extract are mixed, concentrated under reduced pressure, and then freeze-dried to obtain the peach gum extract.

2. The method for preparing peach gum extract according to claim 1, characterized in that: In step (1), the mass ratio of peach gum to water is 1:10-20.

3. The method for preparing peach gum extract according to claim 1, characterized in that: The extraction temperature of the heating reflux extraction in step (1) is 80-100° C. and the extraction time is 1-2 h.

4. The method for preparing peach gum extract according to claim 1, characterized in that: The natural plants described in step (2) are selected from ginseng and / or angelica dahurica.

5. The method for preparing peach gum extract according to claim 1, characterized in that: The extraction temperature of the heating reflux extraction in step (2) is 80-100° C. and the extraction time is 1-2 h.

6. The method for preparing peach gum extract according to claim 1, characterized in that: In step (3), the volume ratio of peach gum extract to natural plant extract is 2-4:

1.

7. The method for preparing peach gum extract according to claim 7, characterized in that: The other active ingredient is Schizophyllan.

8. The peach gum extract prepared by the preparation method according to any one of claims 1 to 7.

9. Use of the peach gum extract according to claim 8 in preparing a product having the effect of increasing the collagen content in the skin.

10. Use of the peach gum extract according to claim 9 in preparing a product having a skin tightening effect.

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