Brown rice enzyme hydrogel mask and preparation method thereof
By using a combination of brown rice enzyme liquid and various traditional Chinese medicine extracts in the facial mask, a hydrogel facial mask with strong antioxidant effect is prepared, which solves the problem of existing facial masks being difficult to resist oxidants and achieves improved skin health and aesthetic effects. goal.
Patent Information
- Application Number
- CN202411732750.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-29
- Publication Date
- 2025-01-21
AI Technical Summary
Existing facial masks are difficult to effectively resist oxidants and other irritants in the external environment, leading to skin aging and health problems.
A skin antioxidant composition containing brown rice enzyme liquid, Ganoderma lucidum extract, licorice extract, sea buckthorn extract and coriander polyphenols is used to prepare a hydrogel mask with antioxidant effects through ultrasound-assisted extraction and mixing.
This mask can effectively resist oxidants, improve skin elasticity and tenderness, resist spots and even out skin tone. After continued use, the skin will become plump and elastic, while ensuring safety and no adverse reactions.
Smart Images
Figure BDA0005160678520000061 
Figure BDA0005160678520000071
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cosmetics, and in particular to a brown rice enzyme hydrogel mask and a preparation method thereof. Background Art
[0002] Smoking, air pollution, water pollution, radiation (x-rays, ultraviolet rays), pesticides, stressful life, excessive exercise and other factors can increase the concentration of free radicals, which is called oxidative stress. The greater the oxidative stress, the higher the concentration of free radicals in the body. Free radicals or oxidants will decompose cells and tissues, affect metabolic functions, cause skin aging and other health problems. Therefore, anti-oxidation is an important step in preventing aging.
[0003] With the continuous development of society, people's pursuit of beauty is also constantly improving, especially the demand for beauty and skin care is constantly expanding. Facial masks have become one of the most commonly used facial skin care products. Antioxidant ingredients are added to facial masks in a certain proportion to make the effective ingredients cooperate with each other and maximize the value of the facial mask. Therefore, adding a composition with skin antioxidant effect to the facial mask to resist oxidants and other irritants in the external environment and maintain the healthy state of the skin is a technical problem to be solved by the present invention. Summary of the invention
[0004] The purpose of the present invention is to provide a brown rice enzyme hydrogel mask and a preparation method thereof. The prepared hydrogel mask has excellent antioxidant effect and can maintain the skin in a healthy state.
[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0006] The invention provides a skin antioxidant composition, comprising the following active ingredients in parts by weight: 10 to 15 parts of brown rice enzyme liquid, 1 to 3 parts of ganoderma lucidum extract, 1 to 3 parts of licorice extract, 1 to 3 parts of sea buckthorn extract, and 1 to 3 parts of coriander polyphenols.
[0007] Preferably, the preparation steps of the brown rice enzyme liquid include:
[0008] (1) brown rice is soaked, germinated, dried and ground to make brown rice powder;
[0009] (2) brown rice powder and glucose are mixed, and prepared into brown rice culture medium after adding water, and yeast and lactic acid bacteria are accessed for fermentation to make brown rice enzyme;
[0010] (3) sterilizing the brown rice enzyme and performing ultrasound-assisted extraction to obtain a brown rice enzyme liquid.
[0011] Preferably, the parameters of the ultrasonic-assisted extraction in step (3) are: extraction temperature of 40-45° C., extraction time of 7-10 min, and extraction power of 300-500 W.
[0012] Preferably, the extraction method of the Ganoderma lucidum extract is: crush the dried Ganoderma lucidum fruiting bodies, add 55% to 95% ethanol by mass, boil and reflux for 1 to 3 hours, continue to extract the filtered residue according to the above steps, extract 3 to 4 times in total, combine the filtrate, and concentrate under reduced pressure to obtain an extract to obtain the Ganoderma lucidum extract.
[0013] Preferably, the preparation method of the licorice extract is: crush the licorice material, add anhydrous ethanol and mix and stir, put it into a kettle, seal and evacuate, and then pump in tetrafluoroethane extraction agent, the extraction temperature is 40-50°C, the extraction time is 50-60min, and after the extraction is completed, the extract is rotary evaporated, concentrated and dried to constant weight to obtain the licorice extract.
[0014] Preferably, the preparation method of the seabuckthorn extract is: seabuckthorn medicinal materials are soaked and then decocted, the filtered medicinal residues are decocted again, the two decoctions are combined and concentrated, ethanol is added to the concentrated solution and stirred, filtered, the filtrate is concentrated again, and the filtrate is further concentrated after passing through a macroporous resin, and the concentrated solution is dried to obtain the seabuckthorn flavonoid extract.
[0015] Preferably, the coriander polyphenols are extracted by microwave-assisted extraction, and the extraction conditions are: extraction time 30-45s, microwave power 50-60W, ethanol volume fraction 60-80%, and solid-liquid ratio 1-3:30-40.
[0016] The present invention also provides a hydrogel mask containing the composition, which also includes the following ingredients in parts by weight: 0.03-0.07 parts of sodium hyaluronate, 3-7 parts of glycerol, 2-4 parts of 1,2-propylene glycol, 0.5-1.5 parts of carbomer 940, 0.1-0.3 parts of ethyl paraben, and 30-70 parts of deionized water.
[0017] The present invention also provides a method for making the hydrogel mask, which is characterized in that it comprises the following steps: pouring carbomer 940, sodium hyaluronate, glycerin, 1,2-propylene glycol, ethylparaben and deionized water into a container and stirring and mixing, stirring evenly at 75-80° C. until they are completely melted into liquid, taking out and cooling to 40-45° C., adding brown rice enzyme liquid, ganoderma lucidum extract, licorice extract, sea buckthorn extract and coriander polyphenols and stirring and mixing, mixing and removing bubbles under the assistance of ultrasound at 40-45° C., and standing and cooling to obtain a brown rice enzyme hydrogel mask.
[0018] The invention also provides a brown rice enzyme hydrogel mask obtained by the preparation method.
[0019] The brown rice enzyme hydrogel mask provided by the present invention has brown rice enzyme liquid as the main component, and is added with four Chinese medicine extracts of ganoderma lucidum extract, licorice extract, sea buckthorn extract, and coriander polyphenols, which integrates various functions. When using the mask of the present invention, the skin absorbs the extract components, effectively resists oxidants in the environment, improves skin elasticity, tenderness, resistance to spots, and even skin color. With continuous use, the skin is plump and smooth. In addition, the present invention is safe and has no adverse reactions while the mask exerts a good efficacy level by rationally proportioning the various raw materials. DETAILED DESCRIPTION
[0020] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0021] Example 1
[0022] A brown rice enzyme hydrogel mask is composed of the following ingredients in parts by mass: 15 parts of brown rice enzyme liquid, 1 part of ganoderma lucidum extract, 1 part of licorice extract, 3 parts of seabuckthorn extract, 3 parts of coriander polyphenols, 0.05 parts of sodium hyaluronate, 5 parts of glycerol, 3 parts of 1,2-propylene glycol, 1 part of carbomer 940, 0.2 parts of ethylparaben, and 40 parts of deionized water.
[0023] Wherein, the preparation steps of brown rice enzyme liquid are:
[0024] (1) Brown rice pretreatment: 100 g of high-quality brown rice was selected, impurities and incomplete grains were removed, washed, soaked in 1 L of distilled water for 12 h, taken out and germinated for 24 h, then dried, ground, and passed through a 60-mesh sieve to make brown rice flour.
[0025] (2) Brown rice fermentation: brown rice flour was added with water to obtain a 6% brown rice flour solution, which was sterilized at 121° C. for 20 min; water was added to glucose to obtain a 6% glucose solution, which was sterilized at 115° C. for 15 min; the brown rice flour solution and the glucose solution were mixed at a volume ratio of 20:1 to prepare a brown rice culture medium, and 2% activated yeast (bacterial content 1×10 8 CFU / g) and 2% activated lactic acid bacteria (bacterial content 5×10 7 CFU / g), and fermented at 30℃ and 120r / min for 12h to make brown rice enzyme.
[0026] (3) Sterilization: Sterilize the brown rice enzyme in a 65°C water bath for 20 min.
[0027] (4) Nutritional enrichment: Brown rice enzyme was extracted with ultrasound assistance at 60°C for 20 min with an extraction power of 150 W.
[0028] (5) Centrifugation: The brown rice enzyme after ultrasound is centrifuged at 4°C and 4000r for 20 min to obtain brown rice enzyme liquid.
[0029] The preparation method of the ganoderma lucidum extract is as follows: taking dried ganoderma lucidum fruiting bodies, crushing them into 30 meshes, adding anhydrous ethanol at a mass volume ratio of 1g:20ml, boiling and refluxing for 2h, filtering, and obtaining a primary filter residue and a primary filtrate; adding an ethanol solution with a mass concentration of 75% to the primary filter residue at a mass volume ratio of 1g:10ml, boiling and refluxing for extraction for 1.5h, filtering, and obtaining a secondary filter residue and a secondary filtrate; adding an ethanol solution with a mass concentration of 55% to the secondary filter residue at a mass volume ratio of 1g:20ml, boiling and refluxing for extraction for 1.5h, filtering, and obtaining a tertiary filter residue and a tertiary filtrate; adding hot water at 95°C to the tertiary filter residue at a mass volume ratio of 1g:20ml, refluxing for 2h, filtering, and obtaining a fourth filter residue and a fourth filtrate; mixing the filtrates obtained from the above four extractions to obtain a ganoderma lucidum extract, and reducing the pressure to concentrate the ganoderma lucidum extract into an extract with a relative density of 1.1, so as to obtain the ganoderma lucidum extract.
[0030] The preparation method of licorice extract is as follows: purchase commercially available licorice slices, mix them evenly, crush them into 20-mesh powder, weigh the powder, add anhydrous ethanol at a mass volume ratio of 10g:1ml, stir, put the evenly stirred material into an extraction kettle, seal it and evacuate it to a pressure of 1.0MPa, pump in a tetrafluoroethane extractant of the same mass as the licorice powder, heat the extraction kettle to a temperature of 40°C, and extract for 60 minutes at this temperature; after the extraction is completed, pump the extract into a separation tank, turn on the compressor to recover tetrafluoroethane, release the extract, rinse the tank with anhydrous ethanol, combine the rinse liquid with the extract, concentrate and recover ethanol with a rotary evaporator, and then dry the concentrate to constant weight with a vacuum drying oven to obtain the licorice extract.
[0031] The preparation method of seabuckthorn extract is as follows: add water 10 times the weight of seabuckthorn medicinal materials, soak for 0.5 hours, decoct for 1 hour, filter, add water 8 times the weight of the medicinal materials to the medicinal residue, decoct for 1 hour, filter out the medicinal liquid, combine the two decoctions, concentrate to a relative density of 1.05 at 60°C, add ethanol to make the volume percentage of alcohol content reach 75%, stir, stand, filter, concentrate the filtrate to a relative density of 1.25 at 65°C, and recover ethanol to obtain a concentrated solution, purify it using a macroporous resin with model D101, add the concentrated solution to a macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume of the eluate, recover the ethanol under reduced pressure, concentrate and dry into powder to obtain the seabuckthorn extract.
[0032] The preparation method of coriander polyphenols is as follows: taking dry rootless coriander and crushing it into 20 meshes, extracting it by microwave-assisted extraction, and the extraction conditions are: extraction time 35s, microwave power 55W, ethanol volume fraction 70%, and solid-liquid ratio 2g:35ml.
[0033] Weigh carbomer 940, sodium hyaluronate, glycerin, 1,2-propylene glycol, ethylparaben and deionized water, pour into a container and stir to mix, stir evenly at 80°C until completely melted into liquid, take out and cool to 40°C, add brown rice enzyme liquid, ganoderma lucidum extract, licorice extract, sea buckthorn extract, and coriander polyphenols according to the components, stir to mix, mix at 40°C, 400W ultrasonic assistance for 9 minutes, remove bubbles, and let stand to cool to obtain a brown rice enzyme hydrogel mask.
[0034] Example 2
[0035] A brown rice enzyme hydrogel mask is composed of the following ingredients in parts by mass: 10 parts of brown rice enzyme liquid, 3 parts of ganoderma lucidum extract, 3 parts of licorice extract, 1 part of seabuckthorn extract, 1 part of coriander polyphenols, 0.03 parts of sodium hyaluronate, 7 parts of glycerol, 2 parts of 1,2-propylene glycol, 1.5 parts of carbomer 940, 0.1 part of ethylparaben, and 60 parts of deionized water.
[0036] The preparation method is the same as that in Example 1.
[0037] Comparative Example 1
[0038] The difference from Example 1 is that Gracilaria polyphenol is used instead of coriander polyphenol.
[0039] The preparation method of Agaricus lemaneiformis polyphenols is as follows: wash and dry the Agaricus lemaneiformis, crush it through a 60-mesh sieve, add 85% ethanol according to a solid-liquid ratio of 1:20, perform ultrasonic-assisted extraction at 70°C for 60 minutes, centrifuge the extract at 5000r / min for 20 minutes, concentrate the supernatant by rotary evaporator, and freeze-dry it in vacuum.
[0040] Comparative Example 2
[0041] The difference from Example 1 is that it does not contain brown rice enzyme.
[0042] Comparative Example 3
[0043] The difference from Example 1 is that no Ganoderma lucidum extract is contained.
[0044] Comparative Example 4
[0045] The difference from Example 1 is that no licorice extract is contained.
[0046] Comparative Example 5
[0047] The difference from Example 1 is that no seabuckthorn extract is contained.
[0048] Comparative Example 6
[0049] The difference from Example 1 is that, in the preparation of the brown rice enzyme liquid, only yeast fermentation is adopted.
[0050] Experimental Example 1
[0051] Free radical scavenging activity assay
[0052] The free radical scavenging activity of the active ingredients in Example 1 and Comparative Examples 1 to 6 (ie, without the addition of sodium hyaluronate, glycerol, 1,2-propylene glycol, carbomer 940, ethyl paraben and deionized water) was determined by the DPPH method.
[0053] The active ingredients in Example 1 and Comparative Examples 1 to 6 (the active ingredients were mixed and diluted with PBS to a concentration of 1%) were placed in 96-well plates, and DPPH prepared with a 100 μM methanol solution was added thereto until the total volume of the solution reached 200 μl.
[0054] After leaving at 37°C for 30 minutes, the sample was measured at a wavelength of 560 nm.
[0055] Free radical scavenging activity (%) = {100-(B / A)}×100
[0056] A: absorbance of the control well to which the sample of the present invention is not added,
[0057] B: absorbance of each group of wells to which the sample of the present invention was added.
[0058] The test results are shown in Table 1.
[0059] Table 1 Antioxidant test results
[0060] Grouping Antioxidant Effect Example 1 78.32％ Comparative Example 1 64.23％ Comparative Example 2 63.26％ Comparative Example 3 74.21％ Comparative Example 4 65.01％ Comparative Example 5 65.91％ Comparative Example 6 53.05％
[0061] As shown in Table 1, the free radical scavenging rate of Example 1 is relatively strong, which indicates that the active ingredients of the present invention are more conducive to scavenging DPPH and achieving a more obvious skin anti-oxidation effect.
[0062] Experimental Example 2
[0063] Cytotoxicity of each group
[0064] The cytotoxicity of the active ingredients in Example 1 and Comparative Examples 1 to 6 (i.e., without the addition of sodium hyaluronate, glycerol, 1,2-propylene glycol, carbomer 940, ethyl paraben and deionized water) was confirmed by the MTT method. First, 5×10 4 L929 cells / well were distributed, and after 24 hours, the active ingredients dissolved in DMSO were added to each group. After 24 hours, the culture medium was removed, and 40 μL of MTT reagent (in PBS 2.5 mg / mL) was added to each well. After reacting in the incubator for 4 hours, the supernatant was removed. 100 μL of DMSO was distributed to each well and completely dissolved. The absorbance at a wavelength of 540 nm was measured using a spectrophotometer. The results are shown in Table 2.
[0065] Table 2 Experimental results of cell survival rate in each group
[0066]
[0067]
[0068] As can be seen from Table 2, each group of active ingredients has no toxic side effects on cells, indicating that the product of the present invention can be used safely.
[0069] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A skin antioxidant composition, characterized in that: The invention comprises the following active ingredients in parts by weight: 10 to 15 parts of brown rice enzyme liquid, 1 to 3 parts of ganoderma lucidum extract, 1 to 3 parts of licorice extract, 1 to 3 parts of sea buckthorn extract and 1 to 3 parts of coriander polyphenols.
2. The composition according to claim 1, characterized in that The preparation steps of the brown rice enzyme liquid include: (1) brown rice is soaked, germinated, dried and ground to make brown rice powder; (2) brown rice powder and glucose are mixed, and prepared into brown rice culture medium after adding water, and yeast and lactic acid bacteria are accessed for fermentation to make brown rice enzyme; (3) sterilizing the brown rice enzyme and performing ultrasound-assisted extraction to obtain a brown rice enzyme liquid.
3. The composition according to claim 2, characterized in that The parameters of the ultrasonic-assisted extraction in step (3) are: extraction temperature of 40-45° C., extraction time of 7-10 min, and extraction power of 300-500 W.
4. The composition according to claim 3, characterized in that The extraction method of the ganoderma lucidum extract is as follows: crushing the dried ganoderma lucidum fruiting bodies, adding 55% to 95% ethanol by mass, boiling and refluxing for 1 to 3 hours, continuing to extract the filtered residue according to the above steps, extracting 3 to 4 times in total, combining the filtrate, and concentrating under reduced pressure to obtain an extract to obtain the ganoderma lucidum extract.
5. The composition according to claim 4, characterized in that The preparation method of the licorice extract is as follows: pulverizing the licorice material, adding anhydrous ethanol to mix and stir, putting it into a kettle, sealing and evacuating, and then pumping in tetrafluoroethane extractant, the extraction temperature is 40-50°C, the extraction time is 50-60min, and after the extraction is completed, the extract is rotary evaporated, concentrated and dried to constant weight to obtain the licorice extract.
6. The composition according to claim 5, characterized in that The preparation method of the seabuckthorn extract comprises the following steps: soaking the seabuckthorn medicinal material and then boiling it, filtering the medicinal residue and boiling it again, combining the two boiling solutions and concentrating them, adding ethanol to the concentrated solution and stirring it, filtering it, concentrating the filtrate again, passing it through a macroporous resin and continuing to concentrate it, and drying the concentrated solution to obtain the seabuckthorn flavonoid extract.
7. The composition according to claim 6, characterized in that The coriander polyphenols are extracted by microwave-assisted extraction, and the extraction conditions are: extraction time 30-45s, microwave power 50-60W, ethanol volume fraction 60-80%, and solid-liquid ratio 1-3:30-40.
8. A hydrogel facial mask comprising the composition according to any one of claims 1 to 7, characterized in that: It also includes the following ingredients in parts by weight: 0.03-0.07 parts of sodium hyaluronate, 3-7 parts of glycerol, 2-4 parts of 1,2-propylene glycol, 0.5-1.5 parts of carbomer 940, 0.1-0.3 parts of ethyl paraben, and 30-70 parts of deionized water.
9. A method for making the hydrogel mask according to claim 8, characterized in that: The method comprises the following steps: pouring carbomer 940, sodium hyaluronate, glycerin, 1,2-propylene glycol, ethylparaben and deionized water into a container and stirring and mixing them; stirring them evenly at 75-80° C. until they are completely melted into liquid; taking them out and cooling them to 40-45° C.; adding brown rice enzyme liquid, ganoderma lucidum extract, licorice extract, sea buckthorn extract and coriander polyphenol and stirring and mixing them; mixing and removing bubbles under the assistance of ultrasound at 40-45° C.; standing and cooling to obtain a brown rice enzyme hydrogel mask.
10. A brown rice enzyme hydrogel mask obtained by the preparation method according to claim 9.