Reduced coenzyme spray, preparation method thereof and reduced coenzyme spray

Abstract

The invention discloses a reduced coenzyme spray, a preparation method thereof and reduced coenzyme spray, and belongs to the technical field of biomedicine. The reduced coenzyme spray is prepared from the following components in parts by weight: 5 to 25 parts of vegetable oil, 0.5 to 5 parts of phospholipid, 75 to 90 parts of water, 1 to 5 parts of ascorbic acid, 0.5 to 3 parts of polyphenol antioxidant, 0.05 to 0.1 part of potassium sorbate and 1 to 10 parts of reduced coenzyme Q10. And carrying out vacuum packaging on the reduced coenzyme spray in a dark place. The reduced coenzyme spray can be smoothly sprayed into the oral cavity of a patient through a spray bottle, sublingual instantaneous absorption can be realized, and the bioavailability of the coenzyme is high.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a reduced coenzyme spray, a preparation method thereof, and a reduced coenzyme spray. Background Art

[0002] Coenzyme is a fat-soluble compound found in eukaryotic organisms that perform aerobic respiration. The coenzyme capsules available on the market are basically made by dissolving coenzyme and soybean oil, beeswax and other substances and then pressing them into pills. The capsules can only be absorbed and utilized after passing through the stomach and intestines, and have low bioavailability and poor efficacy. Therefore, it is necessary to improve the absorption rate of coenzyme. Summary of the invention

[0003] Based on the above-mentioned deficiencies, the present application provides a reduced coenzyme spray, a preparation method thereof and a reduced coenzyme spray to improve the problem of low coenzyme utilization.

[0004] This application is implemented as follows:

[0005] In the first aspect, the example of the present application provides a reduced coenzyme spray, which includes, by weight: 5-25 parts of vegetable oil, 0.5-5 parts of phospholipids, 75-90 parts of water, 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, 0.05-0.1 parts of potassium sorbate and 1-10 parts of reduced coenzyme Q10; the reduced coenzyme spray is light-proof and vacuum-packed.

[0006] In the above implementation process, the reduced coenzyme spray contains 5-25 parts of vegetable oil, 0.5-5 parts of phospholipids, 75-90 parts of water and 1-10 parts of reduced coenzyme Q10, which can form a uniformly mixed liquid preparation for spraying, has good fluidity, and can be directly sprayed into the patient's mouth through a spray bottle. Compared with coenzyme capsules, coenzyme capsules need to enter the stomach and then enter the small intestine for conversion and absorption, with low bioavailability and poor effect, while the reduced coenzyme spray can be sprayed into the patient's mouth through a spray bottle to achieve sublingual instantaneous absorption with high bioavailability. In addition, the use of 0.5-5 parts of phospholipids can not only improve the mixed emulsification effect of vegetable oil and water, improve the smoothness of the reduced coenzyme spray when it is ejected from the nozzle, but also promote the absorption of reduced coenzyme Q10.

[0007] In addition, the reduced coenzyme spray also contains 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, and 0.05-0.1 parts of potassium sorbate. The combination of specific parts of ascorbic acid, polyphenol antioxidants and potassium sorbate can effectively reduce the chance of oxidation of reduced coenzyme Q10 while promoting the body's absorption of reduced coenzyme Q10.

[0008] In combination with the first aspect, in an optional embodiment of the present application, the reduced coenzyme spray further comprises 1-5 parts of vitamin E;

[0009] Optionally, vitamin E is selected from one or more of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol and δ-tocotrienol.

[0010] In the above implementation process, adding vitamin E to the reduced coenzyme spray can reduce the oxidized coenzyme, convert the oxidized coenzyme into the reduced coenzyme, and improve the stability and utilization of the reduced coenzyme spray.

[0011] In combination with the first aspect, in an optional embodiment of the present application, the reduced coenzyme spray further includes 0.5-3 parts of glycerol.

[0012] In the above implementation process, the reduced coenzyme spray is also added with 0.5-3 parts of glycerol, which can further improve the smoothness of the reduced coenzyme spray when spraying.

[0013] In combination with the first aspect, in an optional embodiment of the present application, the reduced coenzyme spray includes: 10-12 parts of vegetable oil, 1-2 parts of vitamin E, 0.5-1 parts of phospholipids, 1-2 parts of glycerol, 77-80 parts of water, 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, 0.05-0.1 parts of potassium sorbate and 3-5 parts of reduced coenzyme Q10.

[0014] In the above implementation process, the reduced coenzyme spray includes 10-12 parts of vegetable oil, 1-2 parts of vitamin E, 0.5-1 parts of phospholipids, 1-2 parts of glycerol, 77-80 parts of water, 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, 0.05-0.1 parts of potassium sorbate and 3-5 parts of reduced coenzyme Q10, which can further improve the ingredient stability of the reduced coenzyme spray, the smoothness of spraying and the absorption and utilization rate of reduced coenzyme Q10.

[0015] In combination with the first aspect, in an optional embodiment of the present application, the vegetable oil is selected from one or more of grape seed oil, pine nut oil, thorn tree oil, walnut oil, coconut oil, palm oil, palm kernel oil, linseed oil, camellia oil, brown rice germ oil, avocado oil, rapeseed oil, rice bran oil, peanut oil, corn oil, wheat germ oil, soybean oil, perilla seed oil, cottonseed oil, sunflower seed oil, kapok seed oil, tuberose oil, shea butter oil, sage oil, cocoa butter, sesame oil or safflower oil.

[0016] In combination with the first aspect, in an optional embodiment of the present application, the phospholipid is selected from one or more of phosphatidylcholine, phosphatidylserine, phosphatidylinositol or acetal phospholipids.

[0017] In combination with the first aspect, in an optional embodiment of the present application, the ascorbic acid is selected from one or more of L-ascorbic acid, rhamnose ascorbic acid, arabinose ascorbic acid, glucose ascorbic acid, fucose ascorbic acid, glucoheptose ascorbic acid, xylose ascorbic acid, galactose ascorbic acid, gulose ascorbic acid, allose ascorbic acid, erythrose ascorbic acid, 6-deoxyascorbic acid and their esters or salts.

[0018] In combination with the first aspect, in an optional embodiment of the present application, the polyphenol antioxidant is selected from one or more of tea polyphenols, cocoa polyphenols, grape polyphenols, grapefruit polyphenols, onion polyphenols, mung bean polyphenols, cowpea polyphenols or sorghum polyphenols.

[0019] In the second aspect, the example of the present application provides a method for preparing the reduced coenzyme spray according to the first aspect, comprising:

[0020] Introducing vegetable oil, lecithin and water into a vacuum emulsifier and mixing them to form an emulsion;

[0021] Pour the emulsion into a dispensing tank, add ascorbic acid, polyphenol antioxidants and potassium sorbate, mix well, and expel the air in the dispensing tank;

[0022] Under an inert atmosphere, add reduced coenzyme Q10 to the preparation tank and stir evenly to dissolve the reduced coenzyme Q10.

[0023] In the above implementation process, vegetable oil, phospholipids and water are first introduced into a vacuum emulsifier to mix and form a uniform emulsion, and then ascorbic acid, polyphenol antioxidants and potassium sorbate are added to make the above raw materials mixed evenly. Then, reduced coenzyme Q10 is added to the preparation tank under an inert atmosphere to obtain a uniformly mixed reduced coenzyme spray, thereby improving the stability of reduced coenzyme Q10.

[0024] In a third aspect, the present application provides a reduced coenzyme spray, comprising a spray bottle and the reduced coenzyme spray provided in the first aspect loaded into the spray bottle.

[0025] In the above implementation process, compared with coenzyme capsules, coenzyme capsules need to enter the stomach and then enter the small intestine for conversion and absorption, with low bioavailability and poor effect, while the reduced coenzyme spray can directly spray the reduced coenzyme spray into the patient's mouth through a spray bottle, achieving instantaneous sublingual absorption, and improving bioavailability. The reduced coenzyme spray provided in the first aspect of the present application has uniform composition, good stability, and can smoothly spray out the spray bottle, which can improve the utilization rate of the reduced coenzyme spray. In addition, the reduced coenzyme spray directly sprays the reduced coenzyme spray into the patient's mouth through a spray bottle, which can solve the oral administration method for children, the elderly, patients with dysphagia, and those who have difficulty in taking water, and increase their compliance with taking. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0027] Figure 1 Schematic diagram of the preparation process of the reduced coenzyme spray provided as an example in this application. DETAILED DESCRIPTION

[0028] The embodiments of the present application will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. In the examples, if specific conditions are not specified, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0029] Coenzyme is a fat-soluble compound present in eukaryotic organisms that perform aerobic respiration. At present, the coenzyme products available on the market are usually coenzyme capsules. Coenzyme capsules are basically made by dissolving coenzyme, soybean oil, beeswax and other substances, making gelatin and then pressing them into pills. After taking coenzyme capsules, the active ingredients in the coenzyme capsules need to pass through the stomach and intestines before they can be absorbed and utilized, with low bioavailability and poor efficacy.

[0030] In addition, the current coenzyme capsule preparation process is complicated. For example, the weighed soybean oil is usually added to the preparation barrel and heated, and then beeswax is added and stirred until the beeswax is completely melted and the liquid is uniform. After cooling, the coenzyme is added and stirred to make it uniform, and then poured into the colloid mill, ground three times, and set aside to sol; then the purified water weighed according to the formula is put into the glue barrel, heated to 70-80°C, and then glycerin is added and stirred evenly, and appropriate amounts of mixed liquid are taken to dissolve titanium dioxide and caramel color respectively, and added to the glue barrel in small amounts and multiple times, and stirred for 10 minutes until uniform; then the weighed gelatin is added, stirred to make it a uniform glue liquid, vacuumed, stopped stirring, and left to stand and vacuumed to eliminate bubbles in the glue liquid, pressurized the glue, and injected the glue liquid into the insulation barrel for insulation and placed, and visually checked for bubbles, and set aside. The subsequent pill pressing, drying, and pill selection processes are complicated and have low production efficiency.

[0031] Therefore, the examples of this application provide a reduced coenzyme spray, a preparation method thereof and a reduced coenzyme spray to improve the problems of poor utilization of coenzyme preparations, complex preparation process of coenzyme preparations and low efficiency.

[0032] In terms of weight, the reduced coenzyme spray provided in the example of the present application includes: 5-25 parts of vegetable oil, 0.5-5 parts of phospholipids, 75-90 parts of water, 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, 0.05-0.1 parts of potassium sorbate and 1-10 parts of reduced coenzyme Q10; the reduced coenzyme spray is light-proof and vacuum-packed.

[0033] The reduced coenzyme spray provided in the example of this application is a liquid preparation that can be directly sprayed into the patient's mouth through a sprayer. Since the reduced coenzyme spray contains 1-10 parts of reduced coenzyme Q10 (ubiquinol reduced coenzyme), the reduced coenzyme Q10 has a high bioavailability and can be directly absorbed sublingually without entering the stomach, small intestine, etc. for conversion and absorption, and has a high absorption efficiency.

[0034] Reduced coenzyme Q10 is easily oxidized. In order to reduce the chance of oxidation of reduced coenzyme Q10, the reduced coenzyme spray provided in the example of the present application is also added with 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, and 0.05-0.1 parts of potassium sorbate. The specific parts of ascorbic acid, polyphenol antioxidants and potassium sorbate cooperate with each other, which can not only improve the antioxidant properties of the reduced coenzyme spray and improve its stability, but also reduce the impact of antioxidants on the performance of reduced coenzyme Q10, promote the absorption of reduced coenzyme Q10, and improve the utilization rate of reduced coenzyme Q10.

[0035] In addition, in order to facilitate the spraying of the reduced coenzyme spray through a spray bottle or the like, reduce the chance of the reduced coenzyme spray being wasted due to clogging of the nozzle when the reduced coenzyme spray is sprayed, and facilitate the human body's absorption of the reduced coenzyme spray and improve the utilization rate of the reduced coenzyme Q10, the reduced coenzyme spray provided in the example of this application also contains 5-25 parts of vegetable oil, 0.5-5 parts of phospholipids and 75-90 parts of water.

[0036] Furthermore, in order to simplify the preparation process of the coenzyme preparation and improve the preparation efficiency of the coenzyme preparation, the present application example also provides a method for preparing a reduced coenzyme spray. The following is a further detailed description of the reduced coenzyme spray in conjunction with the preparation method of the reduced coenzyme spray.

[0037] See also Figure 1 The preparation method of the reduced coenzyme spray comprises:

[0038] S1, emulsification step: introducing the vegetable oil, phospholipids and water weighed according to the ratio into a vacuum emulsifier and mixing them to form an emulsion.

[0039] S2, mixing step: pour the emulsion obtained in step S1 into a preparation tank, add ascorbic acid, polyphenol antioxidant and potassium sorbate weighed according to the proportion, mix well, and exhaust the air in the preparation tank.

[0040] S3, dissolving step: under an inert atmosphere, add the reduced coenzyme Q10 weighed according to the ratio into the preparation tank in step S2, and stir evenly to dissolve the reduced coenzyme Q10.

[0041] Compared with the preparation process of capsule preparations, the preparation method of the reduced coenzyme spray provided in the example of the present application does not require processes such as gel making, pill pressing, drying, and pill selection. The preparation method provided in the example of the present application is simple to operate and is not easy to destroy the effective ingredients of the reduced coenzyme Q10. It can obtain a reduced coenzyme spray with uniform ingredients, stability, good fluidity, and easy spraying.

[0042] In step S1, the vegetable oil can be selected from one or more of grape seed oil, pine nut oil, thorn tree oil, walnut oil, coconut oil, palm oil, palm kernel oil, linseed oil, camellia oil, brown rice germ oil, avocado oil, rapeseed oil, rice bran oil, peanut oil, corn oil, wheat germ oil, soybean oil, perilla seed oil, cottonseed oil, sunflower seed oil, kapok seed oil, tuberose oil, shea butter oil, sage oil, cocoa butter, sesame oil or safflower oil.

[0043] Exemplarily, soybean oil can be used; exemplary, camellia oil can be used; exemplary, grape seed oil can be used; exemplary, coconut oil can be used.

[0044] In step S1, the number of portions of vegetable oil can be one of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 parts, or a range between any two of them.

[0045] Exemplarily, the number of portions of vegetable oil may be 5-10 portions; exemplary, the number of portions of vegetable oil may be 10-12 portions; exemplary, the number of portions of vegetable oil may be 12-20 portions; exemplary, the number of portions of vegetable oil may be 20-25 portions.

[0046] In step S1, adding phospholipids can promote the mixed emulsification effect of vegetable oil and water.

[0047] The type of phospholipid can be selected from one of phosphatidylcholine, phosphatidylserine, phosphatidylinositol and plasmalogen, or a combination of two or more thereof.

[0048] Exemplarily, the phospholipid may be selected from phosphatidylcholine; Exemplarily, the phospholipid may be selected from phosphatidylserine; Exemplarily, the phospholipid may be selected from acetal phospholipids.

[0049] In step S1, the amount of phospholipids can be selected from one of 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts or 5 parts, or a range between any two of them.

[0050] Exemplarily, the number of phospholipids can be selected from 0.5-1 part; Exemplarily, the number of phospholipids can be selected from 1-3 parts; Exemplarily, the number of phospholipids can be selected from 3-5 parts.

[0051] In step S1, water can be selected from deionized water.

[0052] In step S1, the ratio of water to vegetable oil is appropriate, which can improve the dispersion uniformity of various components in the reduced coenzyme preparation. The amount of water can be selected from one of 75 parts, 80 parts, 85 parts or 90 parts or a range between any two of them.

[0053] Furthermore, in step S1, 0.5-3 parts of glycerol may be added, so that the prepared reduced coenzyme spray also includes 0.5-3 parts of glycerol.

[0054] For example, the amount of glycerol added in step S1 may be in the range of 0.5, 1, 1.5, 2, 2.5 or 3, or any two of them.

[0055] Exemplarily, the number of parts of glycerol can be 1 part; exemplary, the number of parts of glycerol can be 0.5-1 part; exemplary, the number of parts of glycerol can be 1-3 parts.

[0056] Furthermore, 1-5 parts of vitamin E can be added in step S1, so that the prepared reduced coenzyme spray also includes 1-5 parts of vitamin E. Adding vitamin E to the reduced coenzyme spray can reduce the oxidized coenzyme, convert the oxidized coenzyme into the reduced coenzyme, and improve the stability and utilization of the reduced coenzyme spray.

[0057] For example, vitamin E can be selected from one or more of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol and δ-tocotrienol.

[0058] Exemplarily, the amount of vitamin E can be one of 1 part, 2 parts, 3 parts, 4 parts, 5 parts, or a range between any two of them.

[0059] In step S2, the ascorbic acid can be selected from one or more of L-ascorbic acid, rhamnose ascorbic acid, arabinose ascorbic acid, glucose ascorbic acid, fucose ascorbic acid, glucoheptose ascorbic acid, xylose ascorbic acid, galactose ascorbic acid, gulose ascorbic acid, allose ascorbic acid, erythrose ascorbic acid, 6-deoxyascorbic acid and their esters or salts.

[0060] Exemplarily, the ascorbic acid may be selected from L-ascorbic acid.

[0061] In step S2, the amount of ascorbic acid can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts or a range between any two parts.

[0062] Exemplarily, the number of ascorbic acid parts can be selected from 1 part; Exemplarily, the number of ascorbic acid parts can be selected from 1-3 parts; Exemplarily, the number of ascorbic acid parts can be selected from 3-5 parts.

[0063] In step S2, the polyphenol antioxidant can be selected from one or more of tea polyphenol, cocoa polyphenol, grape polyphenol, grapefruit polyphenol, onion polyphenol, mung bean polyphenol, cowpea polyphenol or sorghum polyphenol.

[0064] Exemplarily, the polyphenol antioxidants may be selected from grape polyphenols; exemplary, the polyphenol antioxidants may be selected from tea polyphenols; exemplary, the polyphenol antioxidants may be selected from cocoa polyphenols.

[0065] In step S2, the amount of the polyphenol antioxidant may be in the range of 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts or 3 parts, or any two of them.

[0066] Exemplarily, the amount of the polyphenol antioxidant may be 1 part; exemplary, the amount of the polyphenol antioxidant may be 0.5-1 part; exemplary, the amount of the polyphenol antioxidant may be 1-3 parts.

[0067] In step S2, the amount of potassium sorbate can be selected from one of 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, or a range between any two of them.

[0068] Exemplarily, the amount of potassium sorbate can be selected from 0.05 parts; Exemplarily, the amount of potassium sorbate can be selected from 0.05-0.08 parts; Exemplarily, the amount of potassium sorbate can be selected from 0.08-0.1 parts.

[0069] Furthermore, in step S2, the method for discharging the air in the preparation tank includes: introducing nitrogen into the preparation tank to replace the air in the preparation tank.

[0070] In step S3, the inert atmosphere can be selected from a nitrogen atmosphere.

[0071] Furthermore, in step S3, the amount of reduced coenzyme Q10 can be selected from one of 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, or a range between any two of them.

[0072] Exemplarily, the number of reduced coenzyme Q10 parts can be selected from 1-3 parts; exemplary, the number of reduced coenzyme Q10 parts can be selected from 3-5 parts; exemplary, the number of reduced coenzyme Q10 parts can be selected from 5-10 parts.

[0073] Furthermore, after the reduced coenzyme Q10 is dissolved, 0.5-2 parts of the compound seasoning can be added to the preparation tank, and then stirring is continued for 20 minutes and filtered.

[0074] Furthermore, the example of the present application also provides a reduced coenzyme spray, including a spray bottle and a reduced coenzyme spray filled in the spray bottle, and the reduced coenzyme spray is prepared by the above method.

[0075] The present application does not limit the specific structure of the spray bottle. In some possible embodiments, in order to increase the utilization rate of the reduced coenzyme spray in the spray bottle, a vacuum oxygen-free aluminum bottle can be used. The piston of the vacuum oxygen-free aluminum bottle is located below the reduced coenzyme spray in the bottle. When in use, the reduced coenzyme spray gradually rises with the piston; after use, the piston rises to the top of the spray bottle liner, and there is basically no reduced coenzyme spray remaining on the bottle wall.

[0076] The reduced coenzyme spray provided by the present application is further described in detail below in conjunction with the examples.

[0077] Example 1

[0078] Example 1 provides a reduced coenzyme spray, which is prepared by the following preparation method:

[0079] (1) introducing the vegetable oil, vitamin E, phospholipids, glycerol and water weighed according to the formula into a vacuum emulsifier and mixing them to form a more uniform emulsion; wherein the vegetable oil is selected from soybean oil, the number of which is 12 parts; the vitamin E is selected from α-tocotrienol, the number of which is 1 part; the phospholipids are selected from phosphatidylcholine, the number of which is 1 part; the number of glycerol is 1 part; and the number of water is 77.45 parts.

[0080] (2) Pour the above emulsion into a light-proof preparation tank, add ascorbic acid, polyphenol antioxidant and potassium sorbate weighed according to the formula, mix well, and introduce nitrogen to discharge the air in the preparation tank. Wherein, ascorbic acid is selected from L-ascorbic acid, the number of parts is 1; polyphenol antioxidant is selected from cocoa polyphenol, the number of parts is 1; the number of parts of potassium sorbate is 0.05 parts.

[0081] (3) Add reduced coenzyme Q10 to the preparation tank in step (2) under the condition of nitrogen gas isolation and air isolation, and stir evenly to completely dissolve the material. The number of reduced coenzyme Q10 is 5 parts.

[0082] (4) Add compound seasoning under nitrogen flow, continue stirring for 20 minutes, stop stirring, filter the mixture and pour it into a vacuum bottle protected from light. The amount of compound seasoning is 0.5 parts.

[0083] The specific ingredients of the reduced coenzyme spray provided in Example 1 are shown in Table 1.

[0084] Example 2

[0085] Example 2 provides a reduced coenzyme spray, which is different from Example 1 in that, as shown in Table 1, the reduced coenzyme spray provided in Example 2 includes: 10 parts of camellia oil, 1 part of α-tocotrienol, 1 part of phosphatidylserine, 1 part of glycerol, 79.45 parts of water, 1 part of L-ascorbic acid, 1 part of tea polyphenols, 0.05 parts of potassium sorbate, 5 parts of reduced coenzyme Q10, and 0.5 parts of compound seasoning.

[0086] Example 3

[0087] Example 3 provides a reduced coenzyme spray, which is different from Example 1 in that, as shown in Table 1, the reduced coenzyme spray provided in Example 3 includes: 12 parts of grape seed oil, 1 part of α-tocotrienol, 1 part of plasmalogen, 1 part of glycerol, 79.45 parts of water, 1 part of L-ascorbic acid, 1 part of grape polyphenols, 0.05 parts of potassium sorbate, 3 parts of reduced coenzyme Q10, and 0.5 parts of compound seasoning.

[0088] Comparative Example 1

[0089] Comparative Example 1 provides a reduced coenzyme spray, which differs from Example 1 in that:

[0090] In step (2), 1 part of L-ascorbic acid, 1 part of tea polyphenols and 0.05 parts of potassium sorbate are replaced with 2.05 parts of glycyrrhizin flavonoid antioxidant.

[0091] Comparative Example 2

[0092] Comparative Example 2 provides a reduced coenzyme spray, which differs from Example 1 in that no phospholipid is added in step (1).

[0093] Comparative Example 3

[0094] Comparative Example 3 provides a coenzyme spray, which differs from Example 1 in that in step (3), reduced coenzyme Q10 is replaced by coenzyme Q10.

[0095] Comparative Example 4

[0096] Comparative Example 4 provides a reduced coenzyme spray, which differs from Example 1 in that, in step (1), the number of vegetable oils is 30 parts, the number of water is 54.45 parts, and the number of phospholipids is 6 parts.

[0097] Table 1

[0098]

[0099] Test Case

[0100] The existing coenzyme capsule (the raw material of reduced coenzyme Q10 comes from Japan KANEKA, and each capsule contains 30 mg of reduced coenzyme Q10) was used as the control group, and the bioavailability of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and the control group was tested, and the test method was as follows:

[0101] 30 healthy male volunteers aged 27-48 years, with an average weight of 73 ± 7.1 kg and an average height of 172 ± 5.3 cm, were randomly divided into 6 groups. Each volunteer received education and was asked to sign a written consent form on the nature and consequences of the clinical study. The data obtained showed that the results of all volunteers were normal and they were eligible to participate in this study. They were instructed to avoid any type of medication for at least 2 weeks before and during the study. After the subjects fasted overnight, blood samples were collected the next morning, and six groups of subjects were sprayed sublingually 3 times with the coenzyme spray provided by Example 1 of the present application and Comparative Examples 1-3, and the coenzyme capsule provided by the control group. The blood sample collection time was t = 0h (baseline) and 1h, 2h, 3h, 4h, 5h, 6h, 8h, 12h, 24h and 48h after administration. All subjects completed the study.

[0102] Blood samples (2 mL) were drawn into blood collection tubes containing anticoagulant and centrifuged at 1500 rpm for 15 min at 5°C. To ensure that there was no oxygen phase in the sample vials, the labeled, opened sample vials were kept on dry ice before plasma transfer. The sample vials were immediately closed after plasma transfer and frozen at -80°C, then sent to the laboratory on dry ice and stored at -80°C until analysis. Quantification of ubiquinone and ubiquinol in plasma was performed in the laboratory. The extracted samples were vortexed for 30 s, placed on ice for 3 min, and then vortexed again for 30 s. Aggregated proteins were precipitated by centrifugation at full speed in a refrigerated microcentrifuge for 5 min. 100 μL of the supernatant was injected for HPLC analysis using a methanol-propanol gradient method and electrochemical detection.

[0103] The test results are shown in Table 2.

[0104] Table 2

[0105]

[0106]

[0107] In Table 2:

[0108] C max - Maximum plasma concentration of coenzyme Q10 within a specified time span;

[0109] ΔC max - Maximum CoQ10 plasma concentrations above baseline values;

[0110] T max - time to reach peak plasma concentration;

[0111] AUC 48 - the area under the total coenzyme Q10 plasma concentration curve from the time of dosing to the last observation point;

[0112] ΔAUC 48 - The area under the curve for total CoQ10 plasma concentration from the time of dosing to the last observation point was above the baseline value.

[0113] Result analysis:

[0114] As can be seen from Table 2, by comparing Example 1 with the control group, it can be seen that the bioavailability data of the reduced coenzyme spray and coenzyme capsule provided in the examples of this application are compared, and the bioavailability of the reduced coenzyme liquid spray in the oral mucosal instant release form is better than that of the ordinary capsule dosage form.

[0115] By comparing Example 1 with Comparative Example 1, it can be seen that if the antioxidant in the reduced coenzyme spray is adjusted to a common licorice flavonoid antioxidant, the bioavailability of reduced coenzyme Q10 will be affected.

[0116] By comparing Example 1 with Comparative Example 2, it can be seen that if an appropriate amount of phospholipids is not added to the reduced coenzyme spray, the bioavailability of reduced coenzyme Q10 will be affected.

[0117] By comparing Example 1 with Comparative Example 3, it can be seen that if ordinary coenzyme Q10 is used, even if it is made into a spray, it cannot effectively improve the bioavailability of the coenzyme.

[0118] By comparing Example 1 with Comparative Example 4, it can be seen that if the proportions of vegetable oil, water and phospholipids in the reduced coenzyme spray are not matched, the spray effect of the reduced coenzyme spray will be affected, and the bioavailability of reduced coenzyme Q10 will be affected.

[0119] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A reduced coenzyme spray, characterized in that: The reduced coenzyme spray comprises, by weight: 5-25 parts of vegetable oil, 0.5-5 parts of phospholipids, 75-90 parts of water, 1-5 parts of ascorbic acid, 0.5-3 parts of polyphenol antioxidants, 0.05-0.1 parts of potassium sorbate and 1-10 parts of reduced coenzyme Q10; the reduced coenzyme spray is light-proof and vacuum-packed.

2. The reduced coenzyme spray according to claim 1, characterized in that The reduced coenzyme spray also includes 1-5 parts of vitamin E; Optionally, the vitamin E is selected from one or more of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol or δ-tocotrienol.

3. The reduced coenzyme spray according to claim 1, characterized in that The reduced coenzyme spray also includes 0.5-3 parts of glycerol.

4. The reduced coenzyme spray according to claim 1, characterized in that The reduced coenzyme spray comprises: 10-12 parts of the vegetable oil, 1-2 parts of vitamin E, 0.5-1 parts of the phospholipid, 1-2 parts of glycerol, 77-80 parts of the water, 1-5 parts of the ascorbic acid, 0.5-3 parts of the polyphenol antioxidant, 0.05-0.1 parts of potassium sorbate and 3-5 parts of the reduced coenzyme Q10.

5. The reduced coenzyme spray according to any one of claims 1 to 4, characterized in that The vegetable oil is selected from one or more of grape seed oil, pine nut oil, thorn tree oil, walnut oil, coconut oil, palm oil, palm kernel oil, linseed oil, camellia oil, brown rice germ oil, avocado oil, rapeseed oil, rice bran oil, peanut oil, corn oil, wheat germ oil, soybean oil, perilla seed oil, cottonseed oil, sunflower seed oil, kapok seed oil, tuberose oil, shea butter oil, sage oil, cocoa butter, sesame oil or safflower oil.

6. The reduced coenzyme spray according to any one of claims 1 to 4, characterized in that: The phospholipid is selected from one or more of phosphatidylcholine, phosphatidylserine, phosphatidylinositol or plasmalogen.

7. The reduced coenzyme spray according to any one of claims 1 to 4, characterized in that: The ascorbic acid is selected from L-ascorbic acid, rhamnose ascorbic acid, arabinose ascorbic acid, glucose ascorbic acid, fucose ascorbic acid, glucoheptose ascorbic acid, xylose ascorbic acid, galactose ascorbic acid, gulose ascorbic acid, allose ascorbic acid, erythrose ascorbic acid, 6-deoxyascorbic acid, and their esters or salts thereof, or a combination of two or more thereof.

8. The reduced coenzyme spray according to any one of claims 1 to 4, characterized in that: The polyphenol antioxidant is selected from one or a combination of two or more of tea polyphenol, cocoa polyphenol, grape polyphenol, grapefruit polyphenol, onion polyphenol, mung bean polyphenol, cowpea polyphenol or sorghum polyphenol.

9. A method for preparing the reduced coenzyme spray according to any one of claims 1 to 8, characterized in that: include: introducing the vegetable oil, the phospholipid and the water into a vacuum emulsifier and mixing them to form an emulsion; Pour the emulsion into a dispensing tank, add the ascorbic acid, the polyphenol antioxidant and the potassium sorbate, mix well, and exhaust the air in the dispensing tank; Under an inert atmosphere, the reduced coenzyme Q10 is added to the preparation tank and stirred evenly to dissolve the reduced coenzyme Q10.

10. A reduced coenzyme spray, characterized in that: The invention comprises a spray bottle and the reduced coenzyme spray according to any one of claims 1 to 8 loaded in the spray bottle.

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