Application of concentrated birch juice in process of reducing uric acid

Abstract

The invention relates to the technical field of medicines, and discloses an application of concentrated birch juice in a uric acid reduction process, a birch juice separation liquid disclosed by the invention is prepared by an ultrafiltration-nanofiltration three-stage separation and concentration process, and the birch juice separation liquid is composed of substances with relative molecular weight in a range of 1100-2000 dal in birch juice. Wherein the proportion of birch polysaccharide substances is not less than 8%, the prepared birch juice separating medium is low in reducing sugar, low in protein and low in fatty acid, is suitable for being taken by gout patients, can effectively reduce the levels of uric acid and creatinine in serum and the gout arthrocele rate, and can also enable a user to absorb nutrition to the maximum extent.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to application of concentrated birch juice in a process of reducing uric acid. Background Art

[0002] Uric acid is the final product of the metabolism of purine substances in the human body. When the body's uric acid production is much greater than the excretion, the uric acid content in the blood continues to rise and form hyperuricemia, which can cause recurrent inflammation, kidney disease, cardiovascular and cerebrovascular diseases, gout and other symptoms. There are many reasons for abnormal uric acid production, including excessive intake of exogenous purines, excessive production of endogenous purines, and decreased renal function. Excessive intake of exogenous purines can be regulated by paying attention to diet. Excessive production of endogenous purines and decreased renal function often mean that the body is diseased, which makes it more difficult to treat. Among them, excessive production of endogenous purines is mainly caused by abnormal enzyme systems, such as increased activity of xanthine oxidase and excessive activity of phosphoribosyl pyrophosphate synthetase;

[0003] Natural birch sap contains a large amount of reducing sugars such as monosaccharides and disaccharides. When reducing sugars enter the human body, they are metabolized by glucokinase, leading to increased blood uric acid levels, which can induce the onset of gout. At the same time, the proteins, free fatty acids and other components contained in birch sap are not suitable for intake by gout patients. Therefore, it is of great practical significance to remove such components from natural birch sap through separation and purification technology to make it more suitable for the treatment of gout.

[0004] On this basis, after searching the patent website, Chinese patent application number CN202111230107.1 discloses the use of birch sap extract in the treatment of gout;

[0005] It can be seen that there are shortcomings in the above patent application: the existing concentrated birch sap is mainly separated and purified by membrane separation technology during the production process. However, the current method is inefficient and not conducive to rapid production. In addition, other related nutrients in the concentrated birch sap cannot be absorbed more efficiently by the human body, resulting in a single nutrient available for absorption, which affects the subsequent treatment effect.

[0006] In view of the above problems, an application of concentrated birch sap in the process of reducing uric acid is proposed. Summary of the invention

[0007] The purpose of the present invention is to provide an application of concentrated birch sap in the process of reducing uric acid. The device is used to work, thereby solving the problem that the existing concentrated birch sap is mainly separated and purified by membrane separation technology during the production process. However, the currently used method has low efficiency and is not conducive to rapid production. In addition, other related nutrients in the concentrated birch sap cannot be absorbed more efficiently by the human body, resulting in a single nutrient available for absorption, which affects the subsequent treatment effect.

[0008] To achieve the above object, the present invention provides the following technical solution: a birch sap, wherein the birch sap separated liquid is prepared by a method comprising the following steps:

[0009] Step 1: Under the conditions of pressure of 1.2-2.0 MPa, 20-50° C., and flow rate of 1-4 m / s, the birch sap stock solution is subjected to primary separation and purification using an ultrafiltration membrane assembly with a molecular weight cutoff of 1500 dal. After the birch sap stock solution is circulated and ultrafiltered for 4 hours, the filtrate is collected to obtain a birch sap ultrafiltrate having a relative molecular weight of less than 1500 dal;

[0010] Step 2: Using birch sap ultrafiltrate as a raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 700 dal to perform secondary concentration separation on the birch sap ultrafiltrate under the conditions of 1.2-2.0 MPa, 25-55° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 3 hours, collecting the filtrate to obtain a birch sap nanofiltrate with a relative molecular weight of less than 700 dal, and filtering the birch sap ultrafiltrate through a sterilizing filter membrane;

[0011] Step 3: Using birch sap nanofiltrate as raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 1000 dal to perform three-stage concentration separation on the prepared birch sap nanofiltrate under the conditions of 0.5-1.5 MPa, 25-45° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 2 hours, collecting the intercepted liquid to obtain a birch sap separated liquid with a relative molecular weight range of 1100 dal to 2000 dal, and filtering the birch sap nanofiltrate after filtration through a sterilizing filter membrane;

[0012] Step 4: adding citric acid, sugar substitute and water to birch sap, wherein the sugar substitute includes xylitol, the birch sap contains 89.9-93.9%, citric acid 0.1-0.2%, and xylitol: 6-10%;

[0013] Step 5: adding a preservative to the birch sap, wherein the preservative contains phenoxyethanol, and the amount of phenoxyethanol added is 0.8wt%;

[0014] Step 6: The birch juice separation liquid prepared by the ultrafiltration-nanofiltration three-stage separation and concentration process has a polysaccharide content of not less than 10%.

[0015] Preferably, the birch sap fraction consists of substances in birch sap with a relative molecular weight ranging from 1100 dal to 2000 dal.

[0016] Preferably, the total content of polysaccharides in the birch sap extract is not less than 5%, preferably not less than 10%.

[0017] Preferably, the filtration includes filtration using a 0.22 μm filter membrane, and the sterilizing filter membrane is a 0.22 μm filter membrane.

[0018] Preferably, the birch sap nanofiltrate and the birch sap ultrafiltrate are sterilized by ultraviolet light.

[0019] Preferably, the preservative further comprises p-hydroxybenzoic acid or potassium sorbate, the added amount of p-hydroxybenzoic acid is 0.02 wt %, and the added amount of potassium sorbate is 0.015 wt %.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. A kind of concentrated birch sap proposed by the present application uses different sugar substitutes as the replacement of white granulated sugar. While multiple formulas are available for consumers to choose, it is avoided that long-term quoting of beverages containing white granulated sugar causes a series of health problems in users due to high blood sugar in the user's body. In addition, the birch sap in this birch sap beverage is collected and processed from the juice flowing out from the natural birch in Daxing'anling, which contains 17 trace elements such as potassium, phosphorus, calcium, iron, sodium, zinc, 18 kinds of amino acids and fatty acids and multivitamins. People drink it for a long time, not only can strengthen physical fitness and strengthen immunity, but also can eliminate fatigue, and can significantly reduce the uric acid value in the body of patients with high uric acid, thereby overcoming the other related nutrients in the existing concentrated birch sap that cannot be absorbed more efficiently by the human body, resulting in a single nutrition available for absorption, affecting the problem of subsequent therapeutic effect.

[0022] 2. The present application uses a concentrated birch juice, and the filtration and sterilization process is carried out at room temperature, so it is particularly suitable for heat-sensitive materials, etc.; thermal processing has a great impact on the material; and the filtration and separation process belongs to cold sterilization, replacing the traditional pasteurization process that requires heating; the material will not change its properties during the migration process through the filter membrane, so the original flavor of the product can be kept as unchanged as possible, thereby effectively maintaining the color, aroma and nutritional components of the product. There are many filter membranes available for filtration and sterilization, and their materials are mostly nylon, polycarbonate, cellulose acetate, cellulose nitrate, PTFE, ceramics, etc. Commonly used bacteria filters include membrane bacteria filters (0.45μm and 0.22μm pore size). In general, positive pressure filtration is adopted. Positive pressure filtration has the advantages of high flow rate, fast filtration, not easy to be polluted, and can avoid a large number of bubbles generated by protein compared with negative pressure filtration. The sterilizing filter membrane, that is, the sterilized filter membrane, has a pore size of 0.45μm and 0.22μm. A sterilizing filter with a pore size of 0.22 μm can be selected to remove most microbial contamination. The method solves the problem that the existing concentrated birch sap is mainly separated and purified by membrane separation technology during the production process. However, the current method is inefficient and not conducive to rapid production. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0024] Figure 2 This is a comparative structural diagram of mouse joint data of the present invention;

[0025] Figure 3 This is a comparative structural diagram of the mouse serum inflammatory factor data of the present invention;

[0026] Figure 4 It is the overall comparison data structure diagram of the present invention. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings.

[0029] Embodiment 1:

[0030] A birch sap, wherein the birch sap separated liquid is prepared by a method comprising the following steps: using an ultrafiltration membrane assembly with a molecular weight cutoff of 1500 dal to perform primary separation and purification on a birch sap stock solution under the conditions of a pressure of 1.2 to 2.0 MPa, 20 to 50° C., and a flow rate of 1 to 4 m / s, and after the birch sap stock solution is circulated and ultrafiltered for 4 hours, a filtrate is collected to obtain a birch sap ultrafiltrate having an inclusion relative molecular weight of less than 1500 dal;

[0031] Step 2: Using birch sap ultrafiltrate as a raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 700 dal to perform secondary concentration separation on the birch sap ultrafiltrate under the conditions of 1.2-2.0 MPa, 25-55° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 3 hours, collecting the filtrate to obtain a birch sap nanofiltrate with a relative molecular weight of less than 700 dal, and filtering the birch sap ultrafiltrate through a sterilizing filter membrane;

[0032] Step 3: Using birch sap nanofiltrate as raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 1000 dal to perform three-stage concentration separation on the prepared birch sap nanofiltrate under the conditions of 0.5-1.5 MPa, 25-45° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 2 hours, collecting the intercepted liquid to obtain a birch sap separated liquid with a relative molecular weight range of 1100 dal to 2000 dal, and filtering the birch sap nanofiltrate after filtration through a sterilizing filter membrane;

[0033] Step 4: adding citric acid, sugar substitute and water to birch sap, wherein the sugar substitute includes xylitol, the birch sap contains 89.9-93.9%, citric acid 0.1-0.2%, and xylitol: 6-10%;

[0034] Step 5: adding a preservative to the birch sap, wherein the preservative contains phenoxyethanol, and the amount of phenoxyethanol added is 0.8wt%;

[0035] Step 6: The birch juice separation liquid prepared by the ultrafiltration-nanofiltration three-stage separation and concentration process has a polysaccharide content of not less than 10%.

[0036] The polysaccharide content in the birch sap fraction was determined. The difference between the total sugar mass and the reducing sugar mass in the birch sap was the polysaccharide mass in the birch sap.

[0037] Determination of total sugar content in birch sap fractions.

[0038] Preparation of control solution: Place the glucose standard in a drying oven at 60 degrees Celsius for 24 hours. After cooling, weigh 3 mg of the glucose standard and place it in a 15 ml volumetric flask. Add distilled water to dissolve and dilute to the scale. Shake well to obtain a 0.2 mg / ml glucose control solution.

[0039] Pipette 0.2, 0.4, 0.8 and 1.6 ml of glucose control solution into test tubes respectively, make up to 2 ml with distilled water, add 1.5 ml of dilute phenol solution and 2.5 ml of concentrated sulfuric acid respectively, mix well and let cool to room temperature.

[0040] 0.5 ml of the prepared birch sap extract was taken, and distilled water was added to make up to 2 ml. After the same treatment method, the absorbance value of the sample solution was measured, and the total sugar content was measured by referring to the standard curve.

[0041] Determination of reducing sugar mass in birch sap fraction.

[0042] Pipette 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, and 1.2 ml of 1 mg / ml glucose solution into test tubes, add distilled water to 2.0 ml, then add 2.0 ml of DNS solution to each tube, place in a 90-degree constant temperature water bath for 30 minutes, take out and cool, and dilute to 100 ml with distilled water. Draw a standard curve with the concentration of glucose standard as the horizontal axis and the absorbance value as the vertical axis. Pipette 0.5 ml of the prepared birch sap extract, add distilled water to 2 ml, treat in the same way, measure the absorbance value of the sample solution, and refer to the standard curve to measure the reducing sugar content.

[0043] According to experimental determination, in each milliliter of the prepared birch sap extract, the total sugar content is 374.28 mg, the reducing sugar content is 67.37 mg, and the polysaccharide content is 306.91 mg. The content of polysaccharides in the birch sap extract accounts for 36.9%;

[0044] Different sugar substitutes are used as substitutes for white sugar. A variety of formulas are available for consumers to choose from, while avoiding long-term quoting of beverages containing white sugar, which causes high blood sugar in the user's body and leads to a series of health problems for the user. In addition, the birch sap in this birch sap beverage is collected and processed from the sap flowing out of the natural birch in Daxing'anling, which contains 17 trace elements such as potassium, phosphorus, calcium, iron, sodium, zinc, 18 kinds of amino acids and fatty acids and multiple vitamins. Long-term drinking by people can not only enhance physical fitness and strengthen immunity, but also eliminate fatigue, and can significantly reduce the uric acid value in the body of patients with high uric acid, thereby overcoming the problem that other related nutrients in the existing concentrated birch sap cannot be absorbed more efficiently by the human body, resulting in a single nutrient available for absorption, affecting the subsequent treatment effect.

[0045] Embodiment 2:

[0046] Birch sap extract was used to treat gout in rats. Birch sap extract was prepared by referring to the method described in Example 1. 48 male rats were randomly divided into 6 groups, 8 in each group. They were respectively model group, blank group, positive control allopurinol group, and birch sap extract (high, medium and low dose) group. The allopurinol group was intragastrically administered with 5 ml / kg of allopurinol every day, and the high, medium and low dose groups of birch sap extract were intragastrically administered with 15 ml / kg, 10 ml / kg and 5 ml / kg of birch sap every day, respectively. The blank group and the model group were given an equal volume of normal saline, once a day, for 14 consecutive days. On the 7th day, sodium urate crystal injection was injected into the knee joints of the model group, positive control group and birch sap group rats, and the blank group was injected with an equal volume of normal saline.

[0047] The swelling degree of the rat knee joint was measured by using the rope binding method. The circumference of the same part of the rat knee joint was measured before modeling and 12, 24, and 36 hours after modeling. The swelling degree of the knee joint of each group of rats was evaluated by referring to the swelling degree = knee joint circumference after modeling - knee joint circumference before modeling.

[0048] The levels of inflammatory factors in rat serum were detected.

[0049] On the 14th day, rats in each group were anesthetized with 10% chloral hydrate. Blood was drawn from the abdominal aorta into a vacuum collection tube, centrifuged at 60000 r·min-1 for 15 minutes, and the upper serum was taken to determine the content of inflammatory factors by enzyme-linked immunosorbent assay.

[0050] The experimental results showed that the knee joints of the rats in the model group were significantly swollen compared with the blank group at 12, 24, and 36 hours before and after modeling. Compared with the model group, the joint swelling of the rats in the allopurinol group and the birch sap extract group was significantly reduced, indicating that birch sap extract can effectively reduce the swelling of the knee joint caused by gout, and it is dose-related.

[0051] Compared with the blank group, the content of inflammatory factors in the serum of rats in the model group increased significantly. Compared with the model group, the content of inflammatory factors in the birch sap extract group was significantly lower than that in the model group. The results showed that birch sap extract can effectively inhibit the generation of inflammatory factors in rats with gouty arthritis caused by sodium urate, and the effect was most obvious in the high-dose birch sap extract group.

[0052] It should be noted that the above-mentioned electrical components are equipped with power supplies and their control methods are prior art. In order to avoid redundant description, they are uniformly described here; and this application is mainly used to protect mechanical equipment, so the control method and circuit connection are no longer explained in detail. In this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0053] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A concentrated birch sap, characterized in that: The birch sap extract is prepared by a method comprising the following steps: Step 1: Under the conditions of pressure of 1.2-2.0 MPa, 20-50° C., and flow rate of 1-4 m / s, the birch sap stock solution is subjected to primary separation and purification using an ultrafiltration membrane assembly with a molecular weight cutoff of 1500 dal. After the birch sap stock solution is circulated and ultrafiltered for 4 hours, the filtrate is collected to obtain a birch sap ultrafiltrate having a relative molecular weight of less than 1500 dal; Step 2: Using birch sap ultrafiltrate as a raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 700 dal to perform secondary concentration separation on the birch sap ultrafiltrate under the conditions of 1.2-2.0 MPa, 25-55° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 3 hours, collecting the filtrate to obtain a birch sap nanofiltrate with a relative molecular weight of less than 700 dal, and filtering the birch sap ultrafiltrate through a sterilizing filter membrane; Step 3: Using birch sap nanofiltrate as raw material, using a nanofiltration membrane assembly with a molecular weight cutoff of 1000 dal to perform three-stage concentration separation on the prepared birch sap nanofiltrate under the conditions of 0.5-1.5 MPa, 25-45° C., and a flow rate of 2-6 m / s, after circulating nanofiltration separation for 2 hours, collecting the intercepted liquid to obtain a birch sap separated liquid with a relative molecular weight range of 1100 dal to 2000 dal, and filtering the birch sap nanofiltrate after filtration through a sterilizing filter membrane; Step 4: adding citric acid, sugar substitute and water to birch sap, wherein the sugar substitute includes xylitol, the birch sap contains 89.9-93.9%, citric acid 0.1-0.2%, and xylitol: 6-10%; Step 5: adding a preservative to the birch sap, wherein the preservative contains phenoxyethanol, and the amount of phenoxyethanol added is 0.8wt%; Step 6: The birch juice separation liquid prepared by the ultrafiltration-nanofiltration three-stage separation and concentration process has a polysaccharide content of not less than 8%.

2. The concentrated birch sap according to claim 1, characterized in that: The birch sap separated liquid is composed of substances in the birch sap with a relative molecular weight ranging from 1100 dal to 2000 dal.

3. The concentrated birch sap according to claim 1, characterized in that: The total content of polysaccharides in the birch sap extract is not less than 5%, preferably not less than 10%.

4. The concentrated birch sap according to claim 1, characterized in that: The filtration includes filtration using a 0.22 μm filter membrane, and the sterilizing filter membrane is a 0.22 μm filter membrane.

5. The concentrated birch sap according to claim 1, characterized in that: The birch sap nanofiltrate and birch sap ultrafiltrate were sterilized with ultraviolet light.

6. The concentrated birch sap according to claim 1, characterized in that: The preservative further includes p-hydroxybenzoic acid or potassium sorbate, wherein the amount of p-hydroxybenzoic acid added is 0.02 wt % and the amount of potassium sorbate added is 0.015 wt %.

7. The concentrated birch sap according to claim 4, characterized in that: Diseases caused by hyperuricemia include hypertension, hyperlipidemia, atherosclerosis, obesity, insulin resistance, and infectious diseases caused by metabolic disorders caused by hyperuricemia.

8. The concentrated birch sap according to claim 5, characterized in that: For application in diseases, the dosage form of the Chinese herbal combination for lowering uric acid is a tablet, powder, granule, capsule, oral solution or one of the drug forms for injection.

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