Microbiological detection method and device for food quality analysis

Abstract

The invention provides a microbiological detection method and device for food quality analysis, and relates to the technical field of microbiological detection, the device comprises a sterile box, a detector, a stirring and crushing mechanism, an ultraviolet disinfection mechanism and an air inlet disinfection mechanism; the stirring and crushing mechanism comprises a vertical plate fixedly arranged on the rear side of the top of the sterile box, a driving shaft is rotationally connected to the inner side of the vertical plate, a rotating support is fixedly arranged at the front end of the driving shaft, a mixing barrel is fixedly arranged at the bottom of the inner side of the rotating support, and an adjusting screw rod is in threaded connection to the inner side of the top of the rotating support; a sealing cover is rotationally connected to the bottom end of the adjusting screw rod, a rotating rod is vertically and rotationally connected to the inner side of the rotating support, and a bevel gear is fixedly arranged at the top end of the rotating rod. According to the scheme, in the process of stirring and mixing the liquid food and the detection liquid, disinfection and sterilization treatment can be conveniently carried out in the sterile box in a sealed environment at the same time, so that the accuracy of a food microorganism detection result is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and in particular to a microbial detection method and device for food quality analysis. Background Art

[0002] Food microbiological testing is a testing method that uses the theories and methods of microbiology to test the types, quantity, properties of microorganisms in food and their impact on human health, in order to determine whether the food meets the quality standards. Food microbiological testing has extremely strict environmental requirements. The sterile room for microbiological testing must be regularly and strictly disinfected to kill the microorganisms in the sterile room. During the testing process, the testers must ensure that the samples are not contaminated. The non-contamination of samples is the basis for ensuring the accuracy of microbiological testing results.

[0003] Publication No.: CN117070335B discloses a device and method for detecting microbial content based on food safety. Through the mutual cooperation of a sample box, a conduit, a protective cover, a cover plate and a detector, the food is kept in a closed environment during the detection process, thereby ensuring that dust and impurities in the air will not fall into the food sample, preventing the sample from being contaminated, and effectively improving the accuracy and safety of the detection of microbial content in food.

[0004] However, in the process of mixing liquid food with detection liquid to prepare samples, it is not convenient to disinfect the inside of the protective cover in a closed environment. Although the device can detect samples in a sealed environment through the cooperation of the sample box, the conduit, the protective cover, the cover plate and the detector, the protective cover should be in an open state when the device is disinfecting the inside of the protective cover, which will inevitably cause external bacteria, dust, etc. to enter the protective cover with the air, thereby failing to ensure that the inside of the protective cover is in a sterile state when the food is subjected to microbial detection, thereby affecting the accuracy of the food microbial detection results.

[0005] Therefore, it is necessary to provide a microbial detection method and device for food quality analysis to solve the above technical problems. Summary of the invention

[0006] The present invention provides a microbiological detection method and device for food quality analysis, which solves the technical problem that the existing food biological detection device in the related art is not convenient for simultaneously performing disinfection work in a protective cover in a closed environment during sample preparation.

[0007] In order to solve the above technical problems, the microbial detection device for food quality analysis provided by the present invention comprises a sterile box, a detector, a stirring and crushing mechanism, an ultraviolet disinfection mechanism and an air intake disinfection mechanism;

[0008] The stirring and crushing mechanism comprises a vertical plate fixedly arranged on the rear side of the top of the sterile box, the inner side of the vertical plate is rotatably connected with a driving shaft, the front end of the driving shaft is fixedly provided with a rotating bracket, the bottom of the inner side of the rotating bracket is fixedly provided with a mixing barrel, the inner side of the top of the rotating bracket is threadedly connected with an adjusting screw, the bottom end of the adjusting screw is rotatably connected with a sealing cover, the inner side of the rotating bracket is vertically rotatably connected with a rotating rod, the top end of the rotating rod is fixedly provided with a bevel gear, the front side of the vertical plate is fixedly provided with an end face gear, the bevel gear is meshed with the end face gear, the bottom end of the rotating rod and the bottom of the rotating bracket are fixedly provided with a first pulley, the rotating bracket and the inner side of the mixing barrel are vertically rotatably connected with a crushing shaft, the surface of the crushing shaft and the inner side of the mixing barrel are fixedly provided with a crushing knife group, the bottom end of the crushing shaft and the bottom of the rotating bracket are fixedly provided with a second pulley, and the surfaces of the first pulley and the second pulley are sleeved with a first belt;

[0009] The ultraviolet disinfection mechanism comprises a rotating disk rotatably arranged on the inner side of the sterile box, a rotating frame is rotatably connected to the back side of the inner wall of the sterile box, a guide frame is fixedly arranged on the back side of the inner wall of the sterile box, a movable plate is slidably connected to the inner side of the guide frame, an ultraviolet lamp is fixedly arranged on the front side of the movable plate, a third pulley is fixedly arranged on the surface of the driving shaft and located on the back side of the vertical plate, a fourth pulley is fixedly arranged on the rear end of the rotating disk and located on the back side of the sterile box, a second belt is sleeved on the surfaces of the third pulley and the fourth pulley, a protective frame is fixedly arranged on the inner side of the sterile box and located on the outer surfaces of the third pulley and the fourth pulley, and a driving motor for driving the driving shaft to rotate is arranged on the back side of the protective frame;

[0010] The air intake disinfection mechanism includes two sliding frames fixedly arranged on the back side of the inner wall of the sterile box, the inner walls of the two sliding frames are slidably connected with sealing blocks, springs are provided on the separated sides of the two sealing blocks, and the separated ends of the two springs are respectively fixedly connected to the inner walls of the two sliding frames, two vertical rods are fixedly arranged on the top of the movable plate, the surfaces of the two vertical rods are slidably connected to the inner side of the guide frame, two air intake pipes are connected to the inner side of the sterile box and located at the back side of the two sealing blocks, a first gear plate is fixedly arranged on the front side of the movable plate, and two first gears are rotatably connected to the back side of the inner wall of the sterile box, the two first gears are meshed with the first gear plate, and fan blades are fixedly arranged on the front side of the two first gears.

[0011] Preferably, the front side of the rotating disk and the back side of the movable plate are both provided with protrusions for use with the rotating frame, and are slidingly connected to the inner side of the rotating frame through the protrusions. The inner side of the guide frame is provided with grooves for use with the rotating frame and the two vertical rods.

[0012] Preferably, a sample outlet tube is connected to the bottom of the mixing barrel, a sample inlet tube is fixedly arranged on the inner side of the sterile box, and valves are arranged on the surfaces of the sample outlet tube and the sample inlet tube.

[0013] Preferably, a mixing mechanism is fixedly provided on both sides of the inner wall of the sterile box, and the mixing mechanism includes two sliding brackets fixedly provided on both sides of the inner wall of the sterile box, the inner sides of the two sliding brackets are slidably connected with sliders, the tops of the two sliders are fixedly provided with compression springs, the tops of the two compression springs are fixedly connected to the tops of the inner walls of the sliding brackets, the two sliders are rotatably connected with mixing rods on the opposite sides, a mixing rod is fixedly provided on the surface of the mixing rod, and the surfaces of both ends of the mixing rod are rotatably connected with rotating rings, and a rotating shaft is provided on the opposite side of the inner wall of the sterile box for transverse rotation, and two fifth pulleys are fixedly provided on the surface of the rotating shaft, and two sixth pulleys are fixedly provided at both ends of the mixing rod and at the bottom of the two fifth pulleys, the two fifth pulleys and the two sixth pulleys are grouped in pairs, and the surface of each group is sleeved with a third belt, and a rotating disk is fixedly provided on the left end of the rotating shaft and at the left side of the sterile box.

[0014] Preferably, a protective mechanism is fixedly provided on the top of both sides of the inner wall of the sterile box, and the protective mechanism includes two guide brackets fixedly provided on the top of both sides of the inner wall of the sterile box, a movable plate is slidably connected to the inner side of the two guide brackets, second gear plates are fixedly provided on both sides of the top of the movable plate, second gears are fixedly provided at both ends of the rotating shaft and on both sides of the two fifth pulleys, the two second gears are respectively meshed with the two second gear plates, and two protective covers are fixedly provided on the top of the movable plate.

[0015] Preferably, a sample discharging mechanism is fixedly provided at the bottom of the inner wall of the sterile box, and the sample discharging mechanism includes two guide rails fixedly provided at the bottom of the inner wall of the sterile box, and a mobile frame is slidably connected to the top of the two guide rails, and two electric telescopic rods are fixedly provided on the back of the sterile box, and the output ends of the two electric telescopic rods are fixedly connected to the back of the mobile frame, and side panels are fixedly provided on both sides of the top of the mobile frame.

[0016] Preferably, an opening and closing mechanism is fixedly provided at the bottom of the mobile frame, and the opening and closing mechanism includes a first rotating seat fixedly provided at the bottom of the mobile frame, the inner side of the first rotating seat is rotatably connected to two connecting rods, the front ends of the two connecting rods are rotatably connected to the second rotating seat, the front sides of the two second rotating seats are fixedly provided with box doors, the surfaces of the tops of the two second box doors are slidably connected to sliding seats, and the bottom of the sliding seat is fixedly connected to the top of the sterile box.

[0017] Preferably, a testing dish is arranged on the top of the movable frame, and two supporting legs are fixedly arranged on both sides of the bottom of the sterile box.

[0018] A microbial detection method for food quality analysis, used for use of the microbial detection device for food quality analysis, comprises the following steps:

[0019] S1: Preparation for testing

[0020] Before testing food, inspectors must wash their hands with disinfectant or soap, change into sterile clothing and shoes in the buffer room, wear hats, masks, and gloves (or wipe their hands with 70% ethanol several times) to avoid contamination of food, and wipe and disinfect food sampling instruments with alcohol, and then disinfect them with ultraviolet light in the clean bench;

[0021] S2: Preparation and disinfection of food samples

[0022] The liquid food to be tested and the detection liquid are added into the mixing barrel together, and then the driving motor is started. The driving motor drives the rotating bracket to rotate through the driving shaft, and the rotating bracket drives the mixing barrel to rotate, so that the liquid food in the barrel and the detection liquid are mixed, so as to obtain a sample. During the rotation of the rotating bracket, the bevel gear is driven to rotate on the surface of the end gear, so as to drive the first pulley to rotate through the rotating rod, and the first pulley drives the second pulley and the crushing shaft to rotate through the first belt, and the crushing shaft drives the crushing knife group to crush the liquid food, so as to improve the mixing effect of the liquid food and the detection liquid;

[0023] The driving shaft drives the third pulley to rotate during the rotation process, and the third pulley drives the fourth pulley to rotate through the second belt, and the rotating disk is driven to rotate through the fourth pulley. During the rotation process, the rotating disk drives the movable plate and the ultraviolet lamp to move back and forth left and right through the rotating frame, so that the inner wall of the sterile box is disinfected by the ultraviolet lamp;

[0024] The movable plate will drive the vertical rod on the top to move back and forth in the process of moving back and forth, and the two vertical rods will collide with the two sealing blocks to realize the intermittent opening and closing of the air inlet pipe, so that ozone can enter the interior of the sterile box, and the movable plate will drive the first gear plate to move back and forth in the process of moving, and the two first gears will be driven to rotate intermittently through the left and right reciprocating movement of the first gear plate, and the two first gears will be driven to rotate in the process of rotating, so as to improve the diffusion effect of ozone by using the wind force generated when the fan blades rotate;

[0025] S3: Sample testing

[0026] After the food sample is prepared by the mixing barrel, the sample outlet tube and the sample inlet tube are connected by a hose, and the valve is opened after the connection to allow the sample to enter the test dish. After the sample enters the test dish, the rotating handle drives the rotating shaft to rotate, and the rotating shaft drives the two fifth belt pulleys to rotate during the rotation. The two fifth belt pulleys drive the two sixth belt pulleys to rotate through the third belt, and the two sixth belt pulleys further drive the mixing rod and the mixing stick to rotate, and the sample is secondary mixed by the rotation of the mixing stick;

[0027] When the rotating shaft rotates, it will drive the two second gears to rotate, and the two second gear plates will be driven to move backwards through the rotation of the two second gears. When the two second gear plates move, they will drive the moving plate and the protective cover to move backwards, thereby canceling the protection of the detection head of the detector. When the sample completes the secondary mixing, the detector is started, and the sample is detected by the detection head.

[0028] S4: Sample removal and device maintenance

[0029] After the sample is tested, the two electric telescopic rods are started, and the two electric telescopic rods drive the moving frame to slide forward on the inner side of the guide rail, and the moving frame drives the test dish and the sample to move forward to take out the sample. The moving frame drives the side plate to move forward during the movement, and the rotating ring drives the mixing rod and the mixing rod to move upward during the rotation process through the movement of the side plate, so as to separate from the test dish.

[0030] The mobile rack will drive the first rotating seat to move forward during the movement. The movement of the first rotating seat, under the action of the two connecting rods and the second rotating seat, causes the two box doors to slide to both sides on the inner side of the sliding seat, thereby opening the sterile box, so that the mobile rack can drive the test dish and the sample to leave the sterile box, and then take out the test dish and the sample;

[0031] After taking out the test dish and sample, they need to be processed immediately, and the device must be cleaned and disinfected to avoid bacterial growth in the device.

[0032] Compared with the related art, the microbial detection method and device for food quality analysis provided by the present invention have the following beneficial effects:

[0033] In the process of stirring and mixing the liquid food and the test liquid by driving the mixing barrel to rotate through the driving shaft, with the cooperation of the bevel gear and the end gear, the rotation of the crushing shaft and the crushing knife group is used to improve the stirring and mixing effect of the liquid food and the test liquid, and in the process of stirring and mixing the liquid food and the test liquid, the movable plate will drive the ultraviolet lamp to move back and forth left and right to disinfect the sterile box, and in the process of the movable plate driving the ultraviolet lamp to disinfect, it will drive the vertical rod and the first gear plate to move at the same time, so that the air inlet pipe is exposed to ozone, and the fan blades are driven to rotate by the first gear to diffuse the ozone, so as to accelerate the mixing effect of ozone and the air inside the sterile box, thereby improving the sterilization effect inside the sterile box, and in the process of stirring and mixing the liquid food and the test liquid, it is convenient to disinfect and sterilize the sterile box in a sealed environment at the same time, thereby effectively improving the accuracy of the food microorganism detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0035] Figure 1 The best structural diagram provided by the present invention;

[0036] Figure 2 A schematic structural diagram of a cross-sectional view of a sterile box and a mixing barrel provided by the present invention;

[0037] Figure 3 A schematic diagram of the structure of the stirring and crushing mechanism provided by the present invention;

[0038] Figure 4 for Figure 3 The structural schematic diagram of the rotating bracket shown;

[0039] Figure 5 A schematic diagram of the structure of the ultraviolet disinfection mechanism provided by the present invention;

[0040] Figure 6 for Figure 5 The schematic diagram of the state in which the rotating disk drives the rotating frame to rotate, so that the movable plate drives the ultraviolet lamp to move back and forth left and right;

[0041] Figure 7 for Figure 5 A schematic diagram of the structure of the guide frame shown;

[0042] Figure 8 A schematic diagram of the structure of the air intake disinfection mechanism provided by the present invention;

[0043] Fig. 9 for Figure 8 The movable plate shown drives the first gear plate to move back and forth left and right, so that the two air inlet pipes are opened alternately and the two fan blades rotate intermittently;

[0044] Fig.10 A schematic diagram of the structure of the mixing mechanism and the protection mechanism provided by the present invention;

[0045] Fig.11 for Fig.10 The schematic diagram of the state in which the rotating shaft drives the second gear to rotate, so that the second gear plate drives the moving plate and the protective cover to move backward;

[0046] Fig.12 A schematic diagram of the structure of the sample discharging mechanism provided by the present invention;

[0047] Fig.13 for Fig.12 The schematic diagram of the state in which the moving frame drives the side plate to move forward, and the rotating ring drives the rotating shaft to move upward;

[0048] Fig.14 A schematic diagram of the structure of the opening and closing mechanism provided by the present invention;

[0049] Fig.15 for Fig.14 A is shown as an enlarged structural schematic diagram.

[0050] Description of Figure Numbers:

[0051] 1. Sterile box;

[0052] 2. Detector;

[0053] 3. stirring and crushing mechanism; 31. vertical plate; 32. driving shaft; 33. rotating bracket; 34. mixing barrel; 35. adjusting screw; 36. sealing cover; 37. rotating rod; 38. bevel gear; 39. end gear; 310. first pulley; 311. crushing shaft; 312. crushing knife group; 313. second pulley; 314. first belt;

[0054] 4. UV disinfection mechanism; 41. rotating disk; 42. rotating frame; 43. guide frame; 44. movable plate; 45. UV lamp; 46. third pulley; 47. fourth pulley; 48. second belt; 49. protective frame; 410. driving motor;

[0055] 5. air inlet disinfection mechanism; 51. sliding frame; 52. sealing block; 53. spring; 54. vertical rod; 55. air inlet pipe; 56. first gear plate; 57. first gear; 58. fan blade;

[0056] 6. Sample outlet tube; 7. Sample inlet tube;

[0057] 8. Mixing mechanism; 81. Sliding bracket; 82. Sliding block; 83. Compression spring; 84. Mixing rod; 85. Mixing rod; 86. Rotating ring; 87. Rotating shaft; 88. Fifth pulley; 89. Sixth pulley; 810. Third belt; 811. Rotating handle;

[0058] 9. Protective mechanism; 91. Guide bracket; 92. Moving plate; 93. Second gear plate; 94. Second gear; 95. Protective cover;

[0059] 10. Sample discharging mechanism; 101. Guide rail; 102. Moving frame; 103. Electric telescopic rod; 104. Side panel;

[0060] 11. opening and closing mechanism; 111. first rotating seat; 112. connecting rod; 113. second rotating seat; 114. box door; 115. sliding seat;

[0061] 12. Testing dish; 13. Support legs.

[0062] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0063] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0064] The invention provides a microorganism detection method and device for food quality analysis.

[0065] First embodiment:

[0066] See also Figures 1 to 9 , a microbial detection method and device for food quality analysis, comprising a sterile box 1, a detector 2, a stirring and crushing mechanism 3, an ultraviolet disinfection mechanism 4 and an air intake disinfection mechanism 5;

[0067] The stirring and crushing mechanism 3 includes a vertical plate 31 fixedly arranged on the top rear side of the sterile box 1, the inner side of the vertical plate 31 is rotatably connected to a driving shaft 32, a rotating bracket 33 is fixedly arranged at the front end of the driving shaft 32, a mixing barrel 34 is fixedly arranged at the bottom of the inner side of the rotating bracket 33, an adjusting screw 35 is threadedly connected to the inner side of the top of the rotating bracket 33, a sealing cover 36 is rotatably connected to the bottom end of the adjusting screw 35, a rotating rod 37 is vertically rotatably connected to the inner side of the rotating bracket 33, a bevel gear 38 is fixedly arranged at the top of the rotating rod 37, and an end face 34 is fixedly arranged at the front side of the vertical plate 31. The gear 39, the bevel gear 38 is meshed with the end gear 39, the bottom end of the rotating rod 37 and the bottom of the rotating bracket 33 are fixedly provided with a first pulley 310, the rotating bracket 33 is vertically rotatably connected with a crushing shaft 311 on the inner side of the mixing barrel 34, the surface of the crushing shaft 311 and the inner side of the mixing barrel 34 are fixedly provided with a crushing knife group 312, the bottom end of the crushing shaft 311 and the bottom of the rotating bracket 33 are fixedly provided with a second pulley 313, and the surfaces of the first pulley 310 and the second pulley 313 are sleeved with a first belt 314;

[0068] Please combine Figure 3 and Figure 4 : After the liquid food and the detection liquid are put into the mixing barrel 34, the rotation of the driving shaft 32 drives the rotating bracket 33 to rotate, and the rotation of the rotating bracket 33 drives the mixing barrel 34 to rotate, thereby mixing the liquid food and the detection liquid. Compared with the stirring method of the stirring plate, the mixing by the flipping method can effectively improve the mixing effect of the liquid food and the detection liquid;

[0069] Furthermore, when the rotating bracket 33 drives the mixing barrel 34 to rotate, the bevel gear 38 will rotate along with the end gear 39, and the rotation of the bevel gear 38 drives the first pulley 310 to rotate through the rotating rod 37, and the first pulley 310 drives the second pulley 313 and the crushing shaft 311 to rotate through the first belt 314, and the crushing shaft 311 drives the crushing knife group 312 to rotate. The solid in the liquid food can be crushed by the rotation of the crushing knife group 312, thereby improving the mixing effect of the liquid food and the test liquid. When the liquid food does not contain solids, the rotation of the crushing knife group 312 can also improve the mixing effect.

[0070] Preferably, the detector 2 is a prior art and will not be described in detail herein. A thread that cooperates with the adjusting screw 35 is provided on the inner side of the rotating bracket 33. By twisting the adjusting screw 35 upward, the sealing cover 36 can be driven to detach from the mixing barrel 34, thereby facilitating the cleaning and disinfection of the mixing barrel 34. A threaded cover for placing items is provided on the top of the sealing cover 36.

[0071] The ultraviolet disinfection mechanism 4 includes a rotating disk 41 rotatably arranged on the inner side of the sterile box 1, a rotating frame 42 is rotatably connected to the back side of the inner wall of the sterile box 1, a guide frame 43 is fixedly arranged on the back side of the inner wall of the sterile box 1, a movable plate 44 is slidably connected to the inner side of the guide frame 43, an ultraviolet lamp 45 is fixedly arranged on the front side of the movable plate 44, a third pulley 46 is fixedly arranged on the surface of the driving shaft 32 and located on the back side of the vertical plate 31, a fourth pulley 47 is fixedly arranged at the rear end of the rotating disk 41 and located on the back side of the sterile box 1, a second belt 48 is sleeved on the surfaces of the third pulley 46 and the fourth pulley 47, a protective frame 49 is fixedly arranged on the inner side of the sterile box 1 and located on the outer surface of the third pulley 46 and the fourth pulley 47, and a driving motor 410 for driving the driving shaft 32 to rotate is arranged on the back side of the protective frame 49;

[0072] Please combine Figure 5 and Figure 6 : Start the driving motor 410. When the driving motor 410 drives the driving shaft 32 to rotate and mixes the liquid food and the detection liquid, the driving shaft 32 drives the third pulley 46 to rotate. The third pulley 46 drives the fourth pulley 47 and the rotating disk 41 to rotate through the second belt 48. The rotating disk 41 rotates and drives the rotating frame 42 to swing back and forth. The rotating frame 42 drives the movable plate 44 to move back and forth during the swinging process. The movable plate 44 drives the ultraviolet lamp 45 to move back and forth. The reciprocating movement of the ultraviolet lamp 45 can reduce the blind area during disinfection, thereby improving the disinfection effect of the ultraviolet lamp 45.

[0073] Preferably, the ultraviolet lamp 45 is externally connected to a power source, and the use of the ultraviolet lamp 45 is controlled by a control switch.

[0074] The air intake disinfection mechanism 5 includes two sliding frames 51 fixedly arranged on the back of the inner wall of the sterile box 1, the inner walls of the two sliding frames 51 are slidably connected with sealing blocks 52, the separated sides of the two sealing blocks 52 are provided with springs 53, and the separated ends of the two springs 53 are respectively fixedly connected with the inner walls of the two sliding frames 51, two vertical rods 54 are fixedly arranged on the top of the movable plate 44, the surfaces of the two vertical rods 54 are slidably connected with the inner side of the guide frame 43, the inner side of the sterile box 1 and the back sides of the two sealing blocks 52 are connected with two air intake pipes 55, the front side of the movable plate 44 is fixedly provided with a first gear plate 56, the back side of the inner wall of the sterile box 1 is rotatably connected with two first gears 57, the two first gears 57 are meshed with the first gear plate 56, and the front sides of the two first gears 57 are fixedly provided with fan blades 58;

[0075] Please combine Figure 8 and Fig. 9: When the movable plate 44 drives the ultraviolet lamp 45 to move back and forth left and right, it will also drive the vertical rod 54 and the first gear plate 56 to move back and forth left and right. The vertical rod 54 will contact the sealing block 52 during the movement, and after the contact, the vertical rod 54 will continue to move, so as to push the sealing block 52 to expose the air inlet pipe 55, so that the ozone enters the interior of the sterile box 1 through the air inlet pipe 55 to disinfect it. When the ozone enters the sterile box 1, the movable plate 44 will drive the first gear plate 56 to move in the opposite direction, and the movement of the first gear plate 56 will cause the first gear 57 at the bottom to rotate. When the first gear 57 rotates, it will drive the fan blades 58 to rotate. The wind force generated by the rotation of the fan blades 58 can disperse the ozone entering the sterile box 1, thereby improving the efficiency of mixing ozone with the air inside the sterile box 1, and thereby improving the sterilization effect of ozone on the inside of the sterile box 1;

[0076] Preferably, a valve is provided on the surface of the air inlet pipe 55 , and the air inlet pipe 55 is used to be connected to the ozone generator. Ozone can enter the interior of the sterile box 1 through the air inlet pipe 55 . A sealing gasket is provided on the back of the sealing block 52 to enhance the sealing effect of the air inlet pipe 55 .

[0077] The front side of the rotating disk 41 and the back side of the movable plate 44 are both provided with protrusions for use with the rotating frame 42, and are slidably connected to the inner side of the rotating frame 42 through the protrusions. The inner side of the guide frame 43 is provided with grooves for use with the rotating frame 42 and the two vertical rods 54.

[0078] The bottom of the mixing barrel 34 is connected to a sample outlet pipe 6, and a sample inlet pipe 7 is fixedly arranged on the inner side of the sterile box 1. Valves are arranged on the surfaces of the sample outlet pipe 6 and the sample inlet pipe 7.

[0079] Preferably, the sample outlet tube 6 and the sample inlet tube 7 are connected by a hose when in use, but the hose needs to be sterilized before use.

[0080] In this embodiment, unlike the conventional food microorganism detection device, in the process of mixing the liquid food and the detection liquid by driving the mixing barrel 34 to rotate through the driving shaft 32, the device will use the crushing shaft 311 and the crushing knife group 312 to crush the liquid food at the same time through the cooperation of the bevel gear 38 and the end gear 39, so as to effectively improve the mixing effect of the liquid food and the detection liquid. In the process of mixing the liquid food and the detection liquid, the driving shaft 32 will simultaneously drive the rotating disk 41 to rotate through the third pulley 46, the fourth pulley 47 and the second belt 48, so that the movable plate 44 drives the ultraviolet lamp 45 to rotate left and right. It reciprocates right and left to disinfect the sterile box 1, and the movable plate 44 drives the ultraviolet lamp 45 to disinfect, which will drive the vertical rod 54 and the first gear plate 56 to move simultaneously, so that the air inlet pipe 55 is exposed to the ozone, and the fan blades 58 are driven by the first gear 57 to rotate to diffuse the ozone, thereby accelerating the mixing effect of ozone and the air inside the sterile box 1, thereby improving the sterilization effect on the inside of the sterile box 1. In the process of stirring and mixing the liquid food and the test liquid, it is convenient to disinfect and sterilize the sterile box 1 in a sealed environment at the same time, thereby effectively improving the accuracy of the food microorganism detection results.

[0081] Second embodiment:

[0082] See also Fig.10 and Fig.11 A mixing mechanism 8 is fixedly arranged on both sides of the inner wall of the sterile box 1. The mixing mechanism 8 includes two sliding brackets 81 fixedly arranged on both sides of the inner wall of the sterile box 1. The inner sides of the two sliding brackets 81 are slidably connected with sliders 82. The tops of the two sliders 82 are fixedly arranged with compression springs 83. The tops of the two compression springs 83 are fixedly connected to the tops of the inner walls of the sliding brackets 81. The two sliders 82 are rotatably connected to the opposite side with a mixing rod 84. The surface of the mixing rod 84 is fixed with a mixing rod 85. The mixing rods 84 at both ends are fixed with a mixing rod 85. The surfaces are all rotatably connected with a rotating ring 86, a rotating shaft 87 is rotatably arranged on one side opposite to the inner wall of the sterile box 1, two fifth pulleys 88 are fixedly arranged on the surface of the rotating shaft 87, two sixth pulleys 89 are fixedly arranged at both ends of the mixing rod 84 and at the bottom of the two fifth pulleys 88, the two fifth pulleys 88 and the two sixth pulleys 89 are grouped in pairs, and the surface of each group is sleeved with a third belt 810, and a rotating handle 811 is fixedly arranged at the left end of the rotating shaft 87 and located on the left side of the sterile box 1;

[0083] Please combine Fig.10When the sample preparation is completed and needs to be tested, the rotating handle 811 is rotated to drive the rotating shaft 87 to rotate. When the rotating shaft 87 rotates, the two fifth pulleys 88 are driven to rotate at the same time. The two fifth pulleys 88 simultaneously drive the two sixth pulleys 89 to rotate through the two third belts 810. The rotation of the two sixth pulleys 89 drives the mixing rod 84 to rotate. The rotation of the mixing rod 84 drives the mixing rod 85 to perform secondary mixing on the prepared sample, so as to avoid unevenness of the sample during the test after preparation.

[0084] Preferably, a groove cooperating with the slider 82 is provided on the inner side of the sliding bracket 81, so that the slider 82 can slide up and down.

[0085] A protective mechanism 9 is fixedly arranged on the top of both sides of the inner wall of the sterile box 1, and the protective mechanism 9 includes two guide brackets 91 fixedly arranged on the top of both sides of the inner wall of the sterile box 1, and a moving plate 92 is slidably connected to the inner side of the two guide brackets 91, and second gear plates 93 are fixedly arranged on both sides of the top of the moving plate 92, and second gears 94 are fixedly arranged at both ends of the rotating shaft 87 and on both sides of the two fifth pulleys 88, and the two second gears 94 are respectively meshed with the two second gear plates 93, and two protective covers 95 are fixedly arranged on the top of the moving plate 92;

[0086] Please combine Fig.11 : When the rotating shaft 87 drives the fifth pulley 88 to mix the sample, it will drive the second gears 94 on both sides to rotate at the same time, and the two second gears 94 will be driven to move backwards through the rotation of the two second gears 94, and the moving plate 92 and the protective cover 95 will be driven to move backwards through the movement of the two second gear plates 93, and the protection of the protective cover 95 on the detection head of the detector 2 will be cancelled, so as to prepare for the detection of the detector 2;

[0087] Preferably, a groove for cooperating with the movable plate 92 is provided on the inner side of the guide bracket 91, so as to allow the movable plate 92 to slide forward and backward.

[0088] In this embodiment, before the prepared sample is tested by the detector 2, the rotating shaft 87 is driven to rotate by turning the handle 811, and the mixing rod 84 is driven to rotate at the same time under the action of the fifth pulley 88, the sixth pulley 89 and the third belt 810, so that the prepared sample is mixed twice by the mixing rod 85, which can effectively avoid the uneven mixing of the prepared sample during the transportation process, thereby effectively improving the accuracy of the food microorganism detection results, and in the mixing process, the rotating shaft 87 will simultaneously drive the second gear 94 on both sides to rotate, and then drive the movable plate 92 and the protective cover 95 to move backward through the second gear plate 93, thereby canceling the protection of the protective cover 95 on the detection head of the detector 2, and preparing for the detection work of the detector 2.

[0089] Third embodiment:

[0090] See also Figures 12 to 15 A sample discharging mechanism 10 is fixedly arranged at the bottom of the inner wall of the sterile box 1, and the sample discharging mechanism 10 includes two guide rails 101 fixedly arranged at the bottom of the inner wall of the sterile box 1, and a mobile frame 102 is slidably connected to the top of the two guide rails 101, and two electric telescopic rods 103 are fixedly arranged on the back of the sterile box 1, and the output ends of the two electric telescopic rods 103 are fixedly connected to the back of the mobile frame 102, and side panels 104 are fixedly arranged on both sides of the top of the mobile frame 102;

[0091] Please combine Fig.12 and Fig.13 : By controlling the electric telescopic rod 103 to extend forward, the electric telescopic rod 103 will drive the moving frame 102 to slide forward on the inner side of the guide rail 101. Through the movement of the moving frame 102, the tested test dish 12 and the sample can be driven to move forward, so that the test dish 12 is separated from the inside of the sterile box 1. While it is convenient for the test personnel to take it, the number of times the test personnel contact with the inside of the sterile box 1 can be reduced, thereby ensuring the sterility effect inside the sterile box 1;

[0092] Furthermore, the moving frame 102 will drive the side plate 104 to move forward at the same time during the forward movement, and the side plate 104 will move, and the rotating ring 86 will drive the mixing rod 84 to move upward during the rotation, so that the mixing rod 85 will be separated from the test dish 12. During the separation process, the sample contaminated on the surface of the mixing rod 84 and the mixing rod 85 will drip into the test dish 12, thereby avoiding the problem that the sample is difficult to clean and easy to breed bacteria after dripping into the sterile box 1, and conveniently taking out the test dish 12 for replacement and cleaning;

[0093] Preferably, a groove is provided on the inner side of the guide rail 101 for use with the movable frame 102 to facilitate the movable frame 102 to slide forward and backward.

[0094] The bottom of the mobile frame 102 is fixedly provided with an opening and closing mechanism 11, and the opening and closing mechanism 11 includes a first rotating seat 111 fixedly provided at the bottom of the mobile frame 102, the inner side of the first rotating seat 111 is rotatably connected to two connecting rods 112, the front ends of the two connecting rods 112 are rotatably connected to the second rotating seat 113, the front faces of the two second rotating seats 113 are fixedly provided with a box door 114, the top surfaces of the two second box doors 114 are slidably connected to a sliding seat 115, and the bottom of the sliding seat 115 is fixedly connected to the top of the sterile box 1

[0095] Please combine Fig.14 and Fig.15 When the mobile frame 102 moves forward, it will simultaneously drive the first rotating seat 111 at the bottom to move forward. Through the movement of the first rotating seat 111, under the action of the connecting rod 112 and the second rotating seat 113, the two box doors 114 slide to the separated side in the sliding seat 115, so that the two box doors 114 are automatically opened, which is convenient for taking out the test dish 12 and can avoid the contact between the test personnel and the sterile box 1, thereby reducing the probability of bacteria spreading to the inside of the sterile box 1.

[0096] A testing dish 12 is disposed on the top of the mobile frame 102 , and two supporting legs 13 are fixedly disposed on both sides of the bottom of the sterile box 1 .

[0097] In this embodiment, unlike the traditional manual method of taking the test dish 12, the present case drives the test dish 12 to move forward automatically through the moving frame 102. During the movement, the side plate 104 is set, so that the rotating ring 86 can drive the mixing rod 84 and the mixing rod 85 to move upward during the rotation, so that the sample contaminated on the surface of the mixing rod 84 and the mixing rod 85 drips into the test dish 12, thereby avoiding the problem that the sample is difficult to clean and easy to breed bacteria after dripping into the sterile box 1. In addition, the moving frame 102 will simultaneously drive the first rotating seat 111 to move forward during the movement, and then open the box door 114 under the action of the connecting rod 112 and the second rotating seat 113. While facilitating the removal of the test dish 12, it can avoid the contact between the test personnel and the sterile box 1, thereby reducing the probability of bacteria spreading to the inside of the sterile box 1.

[0098] Please refer to Figures 1 to 15 The working principle of the microbial detection device for food quality analysis provided by the present invention is as follows:

[0099] Step S1, firstly, liquid food and detection liquid are put into the mixing barrel 34 through the threaded cover on the top of the sealing cover 36, and then the driving motor 410 is started, and the driving shaft 32 is driven to rotate by the rotation of the driving motor 410, and the driving shaft 32 rotates and then drives the mixing barrel 34 to rotate, and the liquid food and the detection liquid are mixed by the rotation of the mixing barrel 34, and the rotating bracket 33 will simultaneously drive the bevel gear 38 to rotate during the rotation process, and the bevel gear 38 drives the rotating rod 37 to rotate under the action of the end gear 39, and the rotating rod 37 drives the first pulley 310 at the bottom to rotate, and the first pulley 310 drives the second pulley 313 to rotate under the action of the first belt 314, and the second pulley 313 is driven to rotate to drive the crushing shaft 311 and the crushing knife group 312 to rotate, so that the crushing knife group 312 is used to mix and stir the liquid food and the detection liquid in the mixing barrel 34;

[0100] Step S2, the driving shaft 32 will simultaneously drive the third pulley 46 to rotate during the rotation process, and the rotation of the third pulley 46 drives the fourth pulley 47 to rotate under the action of the second belt 48, and the rotation of the fourth pulley 47 drives the rotating disk 41 to rotate, and the rotating disk 41 drives the rotating frame 42 to swing back and forth during the rotation process, and the reciprocating swing of the rotating frame 42 drives the movable plate 44 to move back and forth on the inner side of the guide frame 43, thereby driving the ultraviolet lamp 45 to irradiate back and forth;

[0101] Step S3, during the reciprocating movement of the movable plate 44, the vertical rod 54 and the first gear plate 56 are simultaneously driven to reciprocate. When the vertical rod 54 moves to a certain position to the left or right, it touches the sealing block 52 and pushes the sealing block 52 to a certain position, so that the sealing block 52 cancels the seal of the air inlet pipe 55, allowing ozone to enter the interior of the sterile box 1, and at the same time, the movement of the first gear plate 56 drives the bottom first gear 57 corresponding to the air inlet pipe 55 to rotate, and the rotation of the first gear 57 drives the fan blade 58 to rotate, and the wind force generated by the rotation of the fan blade 58 is used to diffuse the ozone;

[0102] Step S4, when the liquid food and the test liquid are mixed to form a sample, it is first stopped for a period of time to allow the ozone inside the sterile box 1 to dissipate, and then the sample outlet tube 6 and the sample inlet tube 7 are connected through a hose to transport the sample to the test dish 12, and then the rotating handle 811 is rotated, and the rotation of the rotating handle 811 drives the rotating shaft 87 to rotate, and the rotation of the rotating shaft 87 drives the two fifth pulleys 88 to rotate, and the two fifth pulleys 88 drive the two sixth pulleys 89 to rotate under the action of the two third belts 810, and the rotation of the two sixth pulleys 89 drives the mixing rod 84 and the mixing rod 85 to rotate, so that the mixing rod 85 is used to mix the sample in the test dish 12;

[0103] Step S5, when the rotating shaft 87 drives the two fifth pulleys 88 to rotate, the two second gears 94 are driven to rotate at the same time, and the two second gear plates 93 and the moving plate 92 are driven to move backward through the rotation of the two second gears 94. The movement of the moving plate 92 drives the protective cover 95 to cancel the protection of the detection head of the detector 2, so as to prepare for the detection of the detector 2. After the mixing of the sample is completed, the detector 2 is started to detect the sample;

[0104] Step S6, after the sample is tested, the two electric telescopic rods 103 are started, and the two electric telescopic rods 103 drive the moving frame 102 to slide forward on the inner side of the guide rail 101, and the moving frame 102 drives the testing dish 12 on the top to move forward, and the moving frame 102 drives the side plate 104 to move forward during the movement, and the mixing rod 84 is driven to move upward by the movement of the side plate 104 under the action of the rotating ring 86, and at the same time, the slider 82 slides upward on the inner side of the sliding bracket 81, and the compression spring 83 contracts;

[0105] Step S7, the moving frame 102 will drive the first rotating seat 111 at the bottom to move forward during the movement, and under the action of the two connecting rods 112 and the second rotating seat 113, the two box doors 114 will slide to the separated side on the inner side of the sliding seat 115, so as to open the two box doors 114, so that the moving frame 102 drives the test dish 12 out, and then the test personnel will take out the test dish 12, and clean and disinfect the inside of the device, put a new test dish 12 in after disinfection, and close the box door 114.

[0106] Please refer to Figures 1 to 15 , a microbial detection method for food quality analysis, comprising the following steps:

[0107] S1: Preparation for testing

[0108] Before testing food, inspectors must wash their hands with disinfectant or soap, change into sterile clothing and shoes in the buffer room, wear hats, masks, and gloves (or wipe their hands with 70% ethanol several times) to avoid contamination of food, and wipe and disinfect food sampling instruments with alcohol, and then disinfect them with ultraviolet light in the clean bench;

[0109] S2: Preparation and disinfection of food samples

[0110] The liquid food to be tested and the detection liquid are added into the mixing barrel 34 together, and then the driving motor 410 is started. The driving motor 410 drives the rotating bracket 33 to rotate through the driving shaft 32, and the rotating bracket 33 further drives the mixing barrel 34 to rotate, so that the liquid food in the barrel is mixed with the detection liquid, so as to obtain a sample. During the rotation of the rotating bracket 33, the bevel gear 38 is driven to rotate on the surface of the end gear 39, so as to drive the first pulley 310 to rotate through the rotating rod 37, and the first pulley 310 drives the second pulley 313 and the crushing shaft 311 to rotate through the first belt 314, and the crushing shaft 311 further drives the crushing knife group 312 to crush the liquid food, so as to improve the mixing effect of the liquid food and the detection liquid;

[0111] The driving shaft 32 drives the third pulley 46 to rotate during the rotation process, and the third pulley 46 drives the fourth pulley 47 to rotate through the second belt 48, and the rotating disk 41 is driven to rotate through the fourth pulley 47. During the rotation process, the rotating disk 41 drives the movable plate 44 and the ultraviolet lamp 45 to move back and forth left and right through the rotating frame 42, so that the inner wall of the sterile box 1 is disinfected by the ultraviolet lamp 45;

[0112] The movable plate 44 will drive the top vertical rod 54 to move back and forth in the process of moving back and forth. The two vertical rods 54 will collide with the two sealing blocks 52 to achieve intermittent opening and closing of the air inlet pipe 55, so that ozone can enter the interior of the sterile box 1. The movable plate 44 will drive the first gear plate 56 to move back and forth in the process of moving. The left and right reciprocating movement of the first gear plate 56 will drive the two first gears 57 to rotate intermittently. The two first gears 57 will drive the two fan blades 58 to rotate in the process of rotation, so as to use the wind force generated when the fan blades 58 rotate to improve the diffusion effect of ozone.

[0113] S3: Sample testing

[0114] After the food sample is prepared by the mixing barrel 34, the sample outlet tube 6 and the sample inlet tube 7 are connected by a hose, and the valve is opened after the connection to allow the sample to enter the detection dish 12. After the sample enters the detection dish 12, the rotating handle 811 is rotated to drive the rotating shaft 87 to rotate. When the rotating shaft 87 rotates, it drives the two fifth belt pulleys 88 to rotate. The two fifth belt pulleys 88 drive the two sixth belt pulleys 89 to rotate through the third belt 810. The two sixth belt pulleys 89 further drive the mixing rod 84 and the mixing rod 85 to rotate, and the sample is secondary mixed by the rotation of the mixing rod 85.

[0115] During the rotation of the rotating shaft 87, the two second gears 94 are driven to rotate, and the two second gear plates 93 are driven to move backward through the rotation of the two second gears 94. During the movement of the two second gear plates 93, the moving plate 92 and the protective cover 95 are driven to move backward, thereby canceling the protection of the detection head of the detector 2. When the sample completes the secondary mixing, the detector 2 is started, and the sample is detected by the detection head.

[0116] S4: Sample removal and device maintenance

[0117] After the sample is tested, the two electric telescopic rods 103 are started, and the two electric telescopic rods 103 drive the moving frame 102 to slide forward on the inner side of the guide rail 101, and the moving frame 102 drives the testing dish 12 and the sample to move forward, and the sample is taken out. In the process of moving, the moving frame 102 drives the side plate 104 to move forward, and the movement of the side plate 104 causes the rotating ring 86 to drive the mixing rod 84 and the mixing rod 85 to move upward during the rotation process, and separate from the testing dish 12;

[0118] The moving frame 102 drives the first rotating seat 111 to move forward during the movement. The movement of the first rotating seat 111 causes the two box doors 114 to slide to both sides on the inner side of the sliding seat 115 under the action of the two connecting rods 112 and the second rotating seat 113, thereby opening the sterile box 1, so that the moving frame 102 can drive the test dish 12 and the sample to leave the sterile box 1, and then take out the test dish 12 and the sample;

[0119] After the test dish 12 and the sample are taken out, the test dish 12 and the sample need to be processed immediately, and the device needs to be cleaned and disinfected to prevent bacteria from growing in the device.

[0120] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A microbial detection device for food quality analysis, characterized in that: It includes a sterile box, a detector, a stirring and crushing mechanism, an ultraviolet disinfection mechanism and an air intake disinfection mechanism; The stirring and crushing mechanism comprises a vertical plate fixedly arranged on the rear side of the top of the sterile box, the inner side of the vertical plate is rotatably connected with a driving shaft, the front end of the driving shaft is fixedly provided with a rotating bracket, the bottom of the inner side of the rotating bracket is fixedly provided with a mixing barrel, the inner side of the top of the rotating bracket is threadedly connected with an adjusting screw, the bottom end of the adjusting screw is rotatably connected with a sealing cover, the inner side of the rotating bracket is vertically rotatably connected with a rotating rod, the top end of the rotating rod is fixedly provided with a bevel gear, the front side of the vertical plate is fixedly provided with an end face gear, the bevel gear is meshed with the end face gear, the bottom end of the rotating rod and the bottom of the rotating bracket are fixedly provided with a first pulley, the rotating bracket and the inner side of the mixing barrel are vertically rotatably connected with a crushing shaft, the surface of the crushing shaft and the inner side of the mixing barrel are fixedly provided with a crushing knife group, the bottom end of the crushing shaft and the bottom of the rotating bracket are fixedly provided with a second pulley, and the surfaces of the first pulley and the second pulley are sleeved with a first belt; The ultraviolet disinfection mechanism comprises a rotating disk rotatably arranged on the inner side of the sterile box, a rotating frame is rotatably connected to the back side of the inner wall of the sterile box, a guide frame is fixedly arranged on the back side of the inner wall of the sterile box, a movable plate is slidably connected to the inner side of the guide frame, an ultraviolet lamp is fixedly arranged on the front side of the movable plate, a third pulley is fixedly arranged on the surface of the driving shaft and located on the back side of the vertical plate, a fourth pulley is fixedly arranged on the rear end of the rotating disk and located on the back side of the sterile box, a second belt is sleeved on the surfaces of the third pulley and the fourth pulley, a protective frame is fixedly arranged on the inner side of the sterile box and located on the outer surfaces of the third pulley and the fourth pulley, and a driving motor for driving the driving shaft to rotate is arranged on the back side of the protective frame; The air intake disinfection mechanism includes two sliding frames fixedly arranged on the back side of the inner wall of the sterile box, the inner walls of the two sliding frames are slidably connected with sealing blocks, springs are provided on the separated sides of the two sealing blocks, and the separated ends of the two springs are respectively fixedly connected to the inner walls of the two sliding frames, two vertical rods are fixedly arranged on the top of the movable plate, the surfaces of the two vertical rods are slidably connected to the inner side of the guide frame, two air intake pipes are connected to the inner side of the sterile box and located at the back side of the two sealing blocks, a first gear plate is fixedly arranged on the front side of the movable plate, and two first gears are rotatably connected to the back side of the inner wall of the sterile box, the two first gears are meshed with the first gear plate, and fan blades are fixedly arranged on the front side of the two first gears.

2. The microbial detection device for food quality analysis according to claim 1, characterized in that: The front side of the rotating disk and the back side of the movable plate are both provided with protrusions used in conjunction with the rotating frame, and are slidably connected with the inner side of the rotating frame through the protrusions. The inner side of the guide frame is provided with grooves used in conjunction with the rotating frame and the two vertical rods.

3. The microbial detection device for food quality analysis according to claim 1, characterized in that: The bottom of the mixing barrel is connected with a sample outlet tube, the inner side of the sterile box is fixed with a sample inlet tube, and valves are arranged on the surfaces of the sample outlet tube and the sample inlet tube.

4. The microbial detection device for food quality analysis according to claim 1, characterized in that: A mixing mechanism is fixedly provided on both sides of the inner wall of the sterile box, and the mixing mechanism includes two sliding brackets fixedly provided on both sides of the inner wall of the sterile box, the inner sides of the two sliding brackets are slidably connected with sliders, the tops of the two sliders are fixedly provided with compression springs, the tops of the two compression springs are fixedly connected to the tops of the inner walls of the sliding brackets, the two sliders are rotatably connected with mixing rods on the opposite sides, a mixing rod is fixedly provided on the surface of the mixing rod, and the surfaces of both ends of the mixing rod are rotatably connected with rotating rings, and a rotating shaft is provided on the opposite side of the inner wall of the sterile box for horizontal rotation, and two fifth pulleys are fixedly provided on the surface of the rotating shaft, and two sixth pulleys are fixedly provided at both ends of the mixing rod and at the bottom of the two fifth pulleys, the two fifth pulleys and the two sixth pulleys are grouped in pairs, and the third belt is sleeved on the surface of each group, and a rotating handle is fixedly provided on the left end of the rotating shaft and on the left side of the sterile box.

5. The microbial detection device for food quality analysis according to claim 4, characterized in that: A protective mechanism is fixedly provided on the top of both sides of the inner wall of the sterile box, and the protective mechanism includes two guide brackets fixedly provided on the top of both sides of the inner wall of the sterile box, and a movable plate is slidably connected to the inner side of the two guide brackets, and second gear plates are fixedly provided on both sides of the top of the movable plate, and second gears are fixedly provided at both ends of the rotating shaft and on both sides of the two fifth pulleys, and the two second gears are respectively meshed with the two second gear plates, and two protective covers are fixedly provided on the top of the movable plate.

6. The microbial detection device for food quality analysis according to claim 1, characterized in that: A sample discharging mechanism is fixedly provided at the bottom of the inner wall of the sterile box, and the sample discharging mechanism includes two guide rails fixedly provided at the bottom of the inner wall of the sterile box, and a mobile rack is slidably connected to the top of the two guide rails, and two electric telescopic rods are fixedly provided on the back of the sterile box, and the output ends of the two electric telescopic rods are fixedly connected to the back of the mobile rack, and side panels are fixedly provided on both sides of the top of the mobile rack.

7. The microbial detection device for food quality analysis according to claim 6, characterized in that: An opening and closing mechanism is fixedly provided at the bottom of the mobile frame, and the opening and closing mechanism includes a first rotating seat fixedly provided at the bottom of the mobile frame, the inner side of the first rotating seat is rotatably connected to two connecting rods, the front ends of the two connecting rods are rotatably connected to the second rotating seat, the front sides of the two second rotating seats are fixedly provided with box doors, the surfaces of the tops of the two second box doors are slidably connected to sliding seats, and the bottom of the sliding seat is fixedly connected to the top of the sterile box.

8. The microbial detection device for food quality analysis according to claim 6, characterized in that: A testing dish is arranged on the top of the mobile frame, and two supporting legs are fixedly arranged on both sides of the bottom of the sterile box.

9. A microbial detection method for food quality analysis, used for use with the microbial detection device for food quality analysis as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: S1: Preparation for testing Before testing food, inspectors must wash their hands with disinfectant or soap, change into sterile clothing and shoes in the buffer room, wear hats, masks, and gloves (or wipe their hands with 70% ethanol several times) to avoid contamination of food, and wipe and disinfect food sampling instruments with alcohol, and then disinfect them with ultraviolet light in the clean bench; S2: Preparation and disinfection of food samples The liquid food to be tested and the detection liquid are added into the mixing barrel together, and then the driving motor is started. The driving motor drives the rotating bracket to rotate through the driving shaft, and the rotating bracket drives the mixing barrel to rotate, so that the liquid food in the barrel and the detection liquid are mixed, so as to obtain a sample. During the rotation of the rotating bracket, the bevel gear is driven to rotate on the surface of the end gear, so as to drive the first pulley to rotate through the rotating rod, and the first pulley drives the second pulley and the crushing shaft to rotate through the first belt, and the crushing shaft drives the crushing knife group to crush the liquid food, so as to improve the mixing effect of the liquid food and the detection liquid; The driving shaft drives the third pulley to rotate during the rotation process, and the third pulley drives the fourth pulley to rotate through the second belt, and the rotating disk is driven to rotate through the fourth pulley. During the rotation process, the rotating disk drives the movable plate and the ultraviolet lamp to move back and forth left and right through the rotating frame, so that the inner wall of the sterile box is disinfected by the ultraviolet lamp; The movable plate will drive the vertical rod on the top to move back and forth in the process of moving back and forth, and the two vertical rods will collide with the two sealing blocks to realize the intermittent opening and closing of the air inlet pipe, so that ozone can enter the interior of the sterile box, and the movable plate will drive the first gear plate to move back and forth in the process of moving, and the two first gears will be driven to rotate intermittently through the left and right reciprocating movement of the first gear plate, and the two first gears will be driven to rotate in the process of rotating, so as to improve the diffusion effect of ozone by using the wind force generated when the fan blades rotate; S3: Sample testing After the food sample is prepared by the mixing barrel, the sample outlet tube and the sample inlet tube are connected by a hose, and the valve is opened after the connection to allow the sample to enter the test dish. After the sample enters the test dish, the rotating handle drives the rotating shaft to rotate, and the rotating shaft drives the two fifth belt pulleys to rotate during the rotation. The two fifth belt pulleys drive the two sixth belt pulleys to rotate through the third belt, and the two sixth belt pulleys further drive the mixing rod and the mixing stick to rotate, and the sample is secondary mixed by the rotation of the mixing stick; When the rotating shaft rotates, it will drive the two second gears to rotate, and the two second gear plates will be driven to move backwards through the rotation of the two second gears. When the two second gear plates move, they will drive the moving plate and the protective cover to move backwards, thereby canceling the protection of the detection head of the detector. When the sample completes the secondary mixing, the detector is started, and the sample is detected by the detection head. S4: Sample removal and device maintenance After the sample is tested, the two electric telescopic rods are started, and the two electric telescopic rods drive the moving frame to slide forward on the inner side of the guide rail, and the moving frame drives the test dish and the sample to move forward to take out the sample. The moving frame drives the side plate to move forward during the movement, and the rotating ring drives the mixing rod and the mixing rod to move upward during the rotation process through the movement of the side plate, so as to separate from the test dish. The mobile rack will drive the first rotating seat to move forward during the movement. The movement of the first rotating seat, under the action of the two connecting rods and the second rotating seat, causes the two box doors to slide to both sides on the inner side of the sliding seat, thereby opening the sterile box, so that the mobile rack can drive the test dish and the sample to leave the sterile box, and then take out the test dish and the sample; After taking out the test dish and sample, they need to be processed immediately, and the device must be cleaned and disinfected to avoid bacterial growth in the device.

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