Sediment bubble in-situ collection and component detection device and method

Abstract

The invention discloses a sediment bubble in-situ collection and component detection device and method.The method comprises the steps that firstly, a sediment bubble in-situ collection assembly is lowered to the surface of sediment by operating a nylon rope, the sediment is disturbed through a heavy object, and bubbles are released and enter a penicillin bottle through a stainless steel funnel and a silicone tube; then, slowly lifting up the sediment bubble in-situ collection assembly, taking down the penicillin bottle, keeping the opening of the penicillin bottle downward, and covering the penicillin bottle with a cover to complete the bubble collection process; in the gas analysis stage, a sample injector is used for extracting a bubble sample, the bubble sample is put into a second gas collection bottle, gas is fully mixed by a peristaltic pump, and then gas component detection is performed through a mass spectrum analyzer; meanwhile, through water supplement in the first gas collection bottle, it is ensured that no air enters the system, and the detection result is prevented from being interfered; the device can effectively collect and analyze the bubbles in the sediments, and provides a new technical means for researching the interaction between the sediments and the water body and evaluating the environmental risk.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental monitoring, and in particular relates to a device and method for in-situ collection and component detection of sediment bubbles. Background Art

[0002] In various water environments on the earth's surface, the morphology and composition of sediments and their interactions with overlying water bodies directly affect the environmental quality of water bodies and the health of ecosystems. In recent years, with the development of environmental science and geological science, more and more studies have shown that the presence of bubbles in sediments is an important but easily overlooked factor in these interactions. These bubbles may be gases such as methane and carbon dioxide produced by microbial activity in sediments, or they may be formed by external gases entering sediments and being captured. The presence of bubbles not only affects the physical properties of sediments, such as density and porosity, but may also have a significant impact on the chemical and biological processes at the sediment-water interface. Therefore, accurately collecting sediment bubbles and analyzing their components has important scientific significance and practical application value for understanding and monitoring the interaction between sediments and water bodies and assessing environmental risks.

[0003] At present, the collection and analysis of sediment bubbles mainly rely on technical means under laboratory conditions. However, these methods usually require that sediment samples be collected from the field and returned to the laboratory for processing, which may cause the bubbles to escape or undergo composition changes during transportation, affecting the accuracy of the test results. For example, conventional sediment samplers such as columnar samplers and grab samplers can collect relatively complete sediment samples, but during sample extraction and transportation, the bubbles in the sediment are easily disturbed, causing them to escape. In addition, although laboratory desorption and measurement techniques, such as headspace analysis and gas chromatography, can quantitatively analyze bubble components, the accuracy and sensitivity of these methods are limited by the storage and processing methods of the samples. Therefore, there is an urgent need for a new method that can collect sediment bubbles in situ on site and directly detect their components to improve the integrity of bubble collection and the accuracy of analysis results.

[0004] In order to overcome the shortcomings of existing technologies, researchers have proposed a variety of improved in-situ collection and detection methods. For example, some studies have proposed using submersibles or automated underwater robots to carry special collection devices to collect bubbles directly on the surface or inside the sediment. These devices usually include a sealed collection chamber that can capture and preserve bubbles before they escape. However, these methods still face some technical challenges, such as the sealing of the collection device and the flexibility of operation. In addition, in terms of in-situ detection, the technology of direct on-site gas component detection is also gradually developing, such as using miniaturized gas chromatographs or mass spectrometers to directly analyze the collected gases. These in-situ detection technologies can reduce the sample processing steps while improving the detection efficiency and real-time data, but the miniaturization and environmental adaptability of the devices still need to be further optimized. Summary of the invention

[0005] The invention provides a device and method for in-situ collection and component detection of sediment bubbles. The device has a simple structure, is easy to operate, has a high bubble recovery rate, and can accurately analyze gas components in sediment bubbles.

[0006] To solve the above problems, the technical solution provided by the present invention is as follows:

[0007] An embodiment of the present invention provides a device for in-situ collection of sediment bubbles and component detection, the device comprising an in-situ collection component for sediment bubbles and a component detection component;

[0008] The sediment bubble in-situ collection assembly comprises a weight (1), a stainless steel funnel (2), a metal bracket (3), a silicone tube (4), a cillin bottle (5) and a nylon rope (6); the bottom opening of the stainless steel funnel (2) is arranged downward, the metal bracket (3) is welded to the bottom of the stainless steel funnel (2), and the nylon rope (6) is connected to the top of the metal bracket (3); the weight (1) is fixed directly below the bottom of the stainless steel funnel (2); the top of the stainless steel funnel (2) is fixed to the bottom of the stainless steel funnel (2). A silicone tube (4) is sleeved at the small opening of the upper part, and the silicone tube (4) is connected to a vial (5); the weight (1) is used to disturb the sediment to release the bubbles in the sediment; the stainless steel funnel (2) is used to collect the bubbles released from the sediment and guide them into the silicone tube (4) and the vial (5), and the vial (5) is used to seal and store the collected bubble samples; the nylon rope (6) is used to control the up and down movement of the sediment bubble in-situ collection component to disturb the sediment;

[0009] The component detection assembly comprises a first gas collecting bottle (7), a second gas collecting bottle (8), a peristaltic pump (9), an injector (10), a mass spectrometer (11), a three-way pipeline structure (12) and a four-way pipeline structure (13), wherein three interfaces of the three-way pipeline structure (12) are respectively connected to the first gas collecting bottle (7), the second gas collecting bottle (8) and the injector (10), and four interfaces of the four-way pipeline structure (13) are respectively connected to the second gas collecting bottle (8), the peristaltic pump (9), the injector (10) and the mass spectrometer. The analyzer (11) is provided with a first gas collecting bottle (7) filled with water to prevent air from entering the component detection assembly; the second gas collecting bottle (8) is filled with helium to mix and transmit the gas sample; the peristaltic pump (9) is used to provide power during the gas mixing process to ensure that the gas sample is fully mixed in the second gas collecting bottle (8) to obtain a uniform gas sample; the injector (10) is used to extract the sediment bubbles in the vial (5) and inject them into the four-way pipeline structure (13).

[0010] In an optional embodiment of the present invention, the weight (1) and the metal bracket (3) are both made of stainless steel or other corrosion-resistant metals.

[0011] In an optional embodiment of the present invention, the short tube on the right side of the first gas collecting bottle (7) is connected to the air, and the long tube on the left side of the first gas collecting bottle (7) penetrates into the bottom of the bottle and is connected to an interface of the three-way structure (12).

[0012] In an optional embodiment of the present invention, the long tube on the right side of the second gas collecting bottle (8) penetrates into the bottom of the bottle and is connected to an interface of the three-way structure (12); the short tube on the left side of the second gas collecting bottle (8) is connected to an interface of the four-way structure (13).

[0013] An embodiment of the present invention provides a method for in-situ collection and component detection of sediment bubbles, which is implemented by using a device for in-situ collection and component detection of sediment bubbles as in the above embodiment, and includes the following steps:

[0014] Step S1, fix the weight (1) below the stainless steel funnel (2), and put the silicone tube (4) on the upper small mouth of the stainless steel funnel (2); connect the bottle mouth of the syringe bottle (5) to the silicone tube (4), and connect the nylon rope (6) to the top of the metal bracket (3); operate the nylon rope (6), lower the entire sediment bubble in-situ collection assembly to the sediment surface, and disturb the sediment by the heavy impact of the weight (1), so that the bubbles in the sediment are released; the released bubbles enter the syringe bottle (5) through the stainless steel funnel (2) and the silicone tube (4), and the entire sediment bubble in-situ collection assembly is slowly lifted up, and the device is kept vertically downward in water; remove the syringe bottle (5) from the water, keep the bottle mouth downward, and then cover it with a lid; take the entire sediment bubble in-situ collection assembly out of the water, and complete the sediment bubble collection;

[0015] Step S2, using the sample injector (10) to extract 1 ml of sediment bubbles in the syringe bottle (5), and injecting them into the second gas collecting bottle (8) through the heparin cap; rotating the three-way pipe structure (12) and the four-way pipe structure (13) to a preset position to form a closed loop between the second gas collecting bottle (8) and the peristaltic pump (9); turning on the peristaltic pump (9) to fully mix the gas in the second gas collecting bottle (8) to ensure the uniformity of the gas sample; turning off the peristaltic pump (9), rotating the three-way pipe structure (12) and the four-way pipe structure (13) again, automatically aspirating through the mass spectrometer (11), and transporting the gas sample in the second gas collecting bottle (8) to the mass spectrometer (11) for detection.

[0016] In an optional embodiment of the present invention, step S2 further includes: during the mass spectrometry analysis process of the mass spectrometer (11), due to the suction effect, the water in the first gas collecting bottle (7) enters the second gas collecting bottle (8), ensuring that no air enters, thereby avoiding interference with the detection result.

[0017] Compared with the prior art, the embodiments of the present invention provide a device and method for in-situ collection and component detection of sediment bubbles, which have the following beneficial effects: (1) Simple structure: The device has a reasonable structural design and no complex mechanical parts, which is convenient for manufacturing and maintenance. (2) Convenient operation: The up and down movement of the device is controlled by a nylon rope, which is simple to operate and easy to master. The combined use of a peristaltic pump and an injector simplifies the gas collection and mixing process. (3) High bubble recovery rate: The design of the stainless steel funnel and silicone tube can effectively collect the released bubbles, ensure that the bubbles do not escape, and improve the bubble recovery rate. (4) Accurate detection: Helium is used as the carrier gas to avoid interference from external air, and helium itself does not interfere with mass spectrometry analysis, ensuring the accuracy of the detection results. (5) Wide applicability: The device is suitable for sediment bubble collection and analysis in various environments, including lakes, rivers, oceans, etc., and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 A partial structural schematic diagram of a device for in-situ collection and component detection of sediment bubbles provided in an embodiment of the present application.

[0020] Figure 2 Another partial structural schematic diagram of a device for in-situ collection and component detection of sediment bubbles provided in an embodiment of the present application.

[0021] Figure numerals: weight 1, stainless steel funnel 2, metal bracket 3, silicone tube 4, vial 5, nylon rope 6, first gas collecting bottle 7, second gas collecting bottle 8, peristaltic pump 9, injector 10, mass spectrometer 11, three-way pipe structure 12, four-way pipe structure 13. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

[0023] The embodiment of the present invention provides a device and method for in-situ collection and component detection of sediment bubbles, which relates to the field of environmental monitoring technology and aims to improve the integrity of bubble collection and the accuracy of gas component analysis. The device of the present invention has a simple structure and is easy to operate. It can effectively collect and analyze bubbles in sediments, and provides a new technical means for studying the interaction between sediments and water bodies and assessing environmental risks.

[0024] like Figure 1 and Figure 2 As shown, a device for in-situ collection and component detection of sediment bubbles includes an in-situ collection component of sediment bubbles and a component detection component.

[0025] The sediment bubble in-situ collection component includes a weight 1, a stainless steel funnel 2, a metal bracket 3, a silicone tube 4, a syringe bottle 5 and a nylon rope 6. The bottom opening of the stainless steel funnel 2 is set downward, the metal bracket 3 is welded to the bottom of the stainless steel funnel 2, and the nylon rope 6 is connected to the top of the metal bracket 3. The weight 1 is fixed just below the bottom of the stainless steel funnel 2. The small opening on the top of the stainless steel funnel 2 is sleeved with a silicone tube 4, and the silicone tube 4 is connected to the syringe bottle 5. The weight 1 is used to disturb the sediment to release the bubbles in the sediment; the stainless steel funnel 2 is used to collect the bubbles released from the sediment and guide them into the silicone tube 4 and the syringe bottle 5, and the syringe bottle 5 is used to seal and preserve the collected bubble samples; the nylon rope 6 is used to control the up and down movement of the sediment bubble in-situ collection component to disturb the sediment.

[0026] The weight 1 in this embodiment is designed to be moderately heavy, which can effectively disturb the sediment without causing excessive disturbance. The material of the weight can be selected from stainless steel or other corrosion-resistant metals to ensure its durability in an underwater environment. The bottom opening of the stainless steel funnel 2 is downward, located above the weight 1, and can effectively guide the bubbles into the funnel. The size and shape of the funnel are designed according to actual needs to ensure that the bubbles can smoothly enter the funnel and enter the silicone tube 4 through the small opening of the funnel. The metal bracket 3 is connected to the stainless steel funnel 2 by welding, and there are four welding points at the bottom of the stainless steel funnel 2 to ensure the stability of the funnel. The metal bracket 3 is welded and merged into a node ten centimeters above the top of the funnel, thereby ensuring the stability and durability of the entire assembly. The material of the metal bracket 3 can be selected from stainless steel or other corrosion-resistant metals to adapt to long-term underwater working environments. The silicone tube 4 is 5 centimeters long and is sleeved at the small opening of the stainless steel funnel 2 to connect the funnel and the cillin bottle 5. The silicone tube 4 has good sealing properties to ensure that bubbles can pass smoothly without leakage. The vial 5 should have good sealing properties to ensure that the collected bubbles will not escape. The bottle mouth design is easy to disassemble and seal, so as to facilitate the subsequent analysis and preservation of the bubble sample. The nylon rope 6 is connected to the top of the metal bracket 3 to control the up and down movement of the component. The length and strength of the nylon rope 6 are designed according to the collection depth and operation requirements to ensure that the component can operate smoothly in the water and effectively disturb the sediment.

[0027] The component detection assembly includes a first gas collecting bottle 7, a second gas collecting bottle 8, a peristaltic pump 9, an injector 10, a mass spectrometer 11, a three-way pipeline structure 12 and a four-way pipeline structure 13. The three interfaces of the three-way pipeline structure 12 are respectively connected to the first gas collecting bottle 7, the second gas collecting bottle 8 and the injector 10, and the four interfaces of the four-way pipeline structure 13 are respectively connected to the second gas collecting bottle 8, the peristaltic pump 9, the injector 10 and the mass spectrometer 11. The first gas collecting bottle 7 is filled with water to prevent air from entering the component detection assembly. The second gas collecting bottle 8 is filled with helium for mixing and transmitting gas samples. The peristaltic pump 9 is used to provide power during the gas mixing process to ensure that the gas sample is fully mixed in the second gas collecting bottle 8 to obtain a uniform gas sample. The injector 10 is used to extract the sediment bubbles in the syringe bottle 5 and inject them into the four-way pipeline structure 13.

[0028] In this embodiment, the first gas collecting bottle 7 is filled with water, the short tube on the right side of the first gas collecting bottle 7 is connected to the air, the long tube on the left side of the first gas collecting bottle 7 goes deep into the bottom of the bottle and is connected to an interface of the three-way structure 12. The second gas collecting bottle 8 is filled with helium, the long tube on the right side of the second gas collecting bottle 8 goes deep into the bottom of the bottle and is connected to an interface of the three-way structure 12; the short tube on the left side of the second gas collecting bottle 8 is connected to an interface of the four-way structure 13. The air inlet and outlet of the peristaltic pump 9 are connected to the side of the second gas collecting bottle 8 through the four-way pipe structure 13.

[0029] The present invention also provides a method for in-situ collection and component detection of sediment bubbles, which is implemented by using a device for in-situ collection and component detection of sediment bubbles as in the above embodiment, wherein the method comprises the following steps:

[0030] Step S1, fix the weight 1 below the stainless steel funnel 2, and put the silicone tube 4 on the upper small mouth of the stainless steel funnel 2; connect the bottle mouth of the syringe bottle 5 to the silicone tube 4, and connect the nylon rope 6 to the top of the metal bracket 3; operate the nylon rope 6 to lower the entire sediment bubble in-situ collection assembly to the sediment surface, and disturb the sediment through the heavy impact of the weight 1 to release the bubbles in the sediment; the released bubbles enter the syringe bottle 5 through the stainless steel funnel 2 and the silicone tube 4, and the entire sediment bubble in-situ collection assembly is slowly lifted up, and the device is kept vertically downward in the water; remove the syringe bottle 5 in the water, keep the bottle mouth downward, and then cover it with a lid; take the entire sediment bubble in-situ collection assembly out of the water to complete the sediment bubble collection.

[0031] Step S2, use the sample injector 10 to extract 1 ml of sediment bubbles in the syringe bottle 5, and inject it into the second gas collecting bottle 8 through the heparin cap; rotate the three-way pipe structure 12 and the four-way pipe structure 13 to a preset position to form a closed loop between the second gas collecting bottle 8 and the peristaltic pump 9; turn on the peristaltic pump 9 to fully mix the gas in the second gas collecting bottle 8 to ensure the uniformity of the gas sample; turn off the peristaltic pump 9, rotate the three-way pipe structure 12 and the four-way pipe structure 13 again, automatically aspirate through the mass spectrometer 11, and transport the gas sample in the second gas collecting bottle 8 to the mass spectrometer 11 for detection.

[0032] Step S2 also includes: during the mass spectrometry analysis process of the mass spectrometer 11, due to the suction effect, the water in the first gas collecting bottle 7 enters the second gas collecting bottle 8, ensuring that no air enters to avoid interference with the detection result.

[0033] The syringe bottle 5 in this embodiment has good sealing performance, ensuring that the collected bubbles do not escape. The water in the first gas collecting bottle 7 can effectively prevent air from entering the system, ensuring that the collected gas sample is not contaminated by external air. The helium in the second gas collecting bottle 8 is used as a carrier gas to effectively mix and transmit gas samples, and the helium itself does not interfere with mass spectrometry analysis, ensuring the accuracy of the test results. The peristaltic pump 9 is used to provide power during the gas collection and mixing process to ensure that the gas sample is fully mixed in the second gas collecting bottle 8 to obtain a uniform gas sample. The injector 10 is used to extract sediment bubbles and inject them into the four-way pipe structure 13. The amount of gas sample injected can be accurately controlled by the injector 10 to ensure that the amount of gas sample collected each time is consistent, thereby improving the repeatability and accuracy of the test results.

[0034] In summary, although the present invention has been disclosed as above in terms of preferred embodiments, the above preferred embodiments are not intended to limit the present invention. A person skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be based on the scope defined in the claims.

Claims

1. A device for in-situ collection and component detection of sediment bubbles, characterized in that: The device comprises a sediment bubble in-situ collection component and a component detection component; The sediment bubble in-situ collection assembly comprises a weight (1), a stainless steel funnel (2), a metal bracket (3), a silicone tube (4), a cillin bottle (5) and a nylon rope (6); the bottom opening of the stainless steel funnel (2) is arranged downward, the metal bracket (3) is welded to the bottom of the stainless steel funnel (2), and the nylon rope (6) is connected to the top of the metal bracket (3); the weight (1) is fixed directly below the bottom of the stainless steel funnel (2); the top of the stainless steel funnel (2) is fixed to the bottom of the stainless steel funnel (2). A silicone tube (4) is sleeved at the small opening of the upper part, and the silicone tube (4) is connected to a vial (5); the weight (1) is used to disturb the sediment to release the bubbles in the sediment; the stainless steel funnel (2) is used to collect the bubbles released from the sediment and guide them into the silicone tube (4) and the vial (5), and the vial (5) is used to seal and store the collected bubble samples; the nylon rope (6) is used to control the up and down movement of the sediment bubble in-situ collection component to disturb the sediment; The component detection assembly comprises a first gas collecting bottle (7), a second gas collecting bottle (8), a peristaltic pump (9), an injector (10), a mass spectrometer (11), a three-way pipeline structure (12) and a four-way pipeline structure (13), wherein three interfaces of the three-way pipeline structure (12) are respectively connected to the first gas collecting bottle (7), the second gas collecting bottle (8) and the injector (10), and four interfaces of the four-way pipeline structure (13) are respectively connected to the second gas collecting bottle (8), the peristaltic pump (9), the injector (10) and the mass spectrometer. The analyzer (11) is provided with a first gas collecting bottle (7) filled with water to prevent air from entering the component detection assembly; the second gas collecting bottle (8) is filled with helium to mix and transmit the gas sample; the peristaltic pump (9) is used to provide power during the gas mixing process to ensure that the gas sample is fully mixed in the second gas collecting bottle (8) to obtain a uniform gas sample; the injector (10) is used to extract the sediment bubbles in the vial (5) and inject them into the four-way pipeline structure (13).

2. The device for in-situ collection and component detection of sediment bubbles according to claim 1, characterized in that: The weight (1) and the metal bracket (3) are both made of stainless steel or other corrosion-resistant metals.

3. The device for in-situ collection and component detection of sediment bubbles according to claim 1, characterized in that: The short tube on the right side of the first gas collecting bottle (7) is connected to the air, and the long tube on the left side of the first gas collecting bottle (7) penetrates into the bottom of the bottle and is connected to an interface of the three-way structure (12).

4. The device for in-situ collection and component detection of sediment bubbles according to claim 1, characterized in that: The long tube on the right side of the second gas collecting bottle (8) penetrates into the bottom of the bottle and is connected to an interface of the three-way structure (12); the short tube on the left side of the second gas collecting bottle (8) is connected to an interface of the four-way structure (13).

5. A method for in-situ collection and component detection of sediment bubbles, implemented by using a device for in-situ collection and component detection of sediment bubbles as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: Step S1, fix the weight (1) below the stainless steel funnel (2), and put the silicone tube (4) on the upper small mouth of the stainless steel funnel (2); connect the bottle mouth of the syringe bottle (5) to the silicone tube (4), and connect the nylon rope (6) to the top of the metal bracket (3); operate the nylon rope (6), lower the entire sediment bubble in-situ collection assembly to the sediment surface, and disturb the sediment by the heavy impact of the weight (1), so that the bubbles in the sediment are released; the released bubbles enter the syringe bottle (5) through the stainless steel funnel (2) and the silicone tube (4), and the entire sediment bubble in-situ collection assembly is slowly lifted up, and the device is kept vertically downward in water; remove the syringe bottle (5) from the water, keep the bottle mouth downward, and then cover it with a lid; take the entire sediment bubble in-situ collection assembly out of the water, and complete the sediment bubble collection; Step S2, using the sample injector (10) to extract 1 ml of sediment bubbles in the syringe bottle (5), and injecting them into the second gas collecting bottle (8) through the heparin cap; rotating the three-way pipe structure (12) and the four-way pipe structure (13) to a preset position to form a closed loop between the second gas collecting bottle (8) and the peristaltic pump (9); turning on the peristaltic pump (9) to fully mix the gas in the second gas collecting bottle (8) to ensure the uniformity of the gas sample; turning off the peristaltic pump (9), rotating the three-way pipe structure (12) and the four-way pipe structure (13) again, automatically aspirating through the mass spectrometer (11), and transporting the gas sample in the second gas collecting bottle (8) to the mass spectrometer (11) for detection.

6. The method for in-situ collection and component detection of sediment bubbles according to claim 5, characterized in that: Step S2 also includes: during the mass spectrometry analysis process of the mass spectrometer (11), due to the suction effect, the water in the first gas collecting bottle (7) enters the second gas collecting bottle (8), ensuring that no air enters to avoid interference with the detection result.

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