Shaped image imaging system

Abstract

A visible imaging system includes a liquid injection mechanism, a detection mechanism, and a flow cell assembly. The liquid injection mechanism comprises an electromagnetic valve unit, an injector unit and a motor assembly, and the injector unit comprises a cleaning pump assembly, a sheath liquid pump assembly, a sample laminar flow pump assembly and a sample suction pump assembly. The detection mechanism comprises a substrate, a flow cell assembly, an illumination module and an imaging module. The flow cell assembly includes a flow cell bracket and a flow cell. And the electromagnetic valve unit is connected with the injector unit, so that a stable laminar flow effect of a sample can be formed in the flow channel. The flow cell is integrally formed by adopting colorless high-transmittance glass, so that the influence of air tightness and light transmittance on imaging is avoided. And the thickness of the laminar flow is limited to be 4 + / -0.5 microns, so that the front side of a characteristic surface with formed components can face the imaging module, and the imaging module can shoot a high-definition image.

Description

Technical Field

[0001] The present invention relates to the technical field of medical diagnostic equipment, in particular to a shaped component imaging system. Background Art

[0002] Currently, in all medical fields at home and abroad, there are only two technologies for automatic urine detection equipment, namely: planar flow technology and urine sedimentation detection technology.

[0003] The existing detection technology has the following problems:

[0004] 1. Due to differences in control, both technologies have unclear imaging.

[0005] 2. The essence of sedimentation is to centrifuge the liquid, that is, there will be a phenomenon of superposition of formed elements. Because various formed elements overlap, the accuracy of photo recognition is greatly reduced.

[0006] 3. The types of formed ingredients that can be detected by sedimentation are limited, all below 9.

[0007] 4. Due to defects in the control method, the planar flow type leads to unstable laminar flow, and the image is sometimes clear and sometimes blurred.

[0008] 5. Problems with the raw materials of the flow cell used in planar flow technology (specifically, the use of colored materials resulting in low transparency, and problems with the viscose process resulting in the flow cell channel not being sealed, thus causing unstable laminar flow), leading to blurred imaging and the inability to identify formed components.

[0009] In Chinese patent CN2018112976713, a flow cell mechanism adjustment device for a fully automatic urine formed component analyzer is disclosed, which is composed of a motor base plate, an optical coupling bracket, a bearing seat, a flanged bearing, an optical coupling bracket, a lateral adjustment plate, a lateral rotating block, a liner pressing block, a flow cell positioning cover, a flow cell, a silicone pad, a flow cell liner, etc., and is driven by a stepper motor, a lead screw transmission with an anti-backlash nut (eliminating the gap), and a linear guide rail to control the flow cell to move along the optical axis and automatically find the focus of the optical system. It cannot eliminate the defects of the flow cell itself, that is, the flow cell has low light transmittance, poor imaging, unstable laminar flow, etc. Summary of the invention

[0010] In view of this, the present invention provides a formed component imaging system to solve the above technical problems.

[0011] A formed component imaging system, comprising a liquid injection mechanism, a detection mechanism, and a flow cell assembly arranged in the detection mechanism, the detection mechanism comprising an imaging module, the flow cell assembly comprising a flow cell and a sample nozzle arranged on the flow cell, the flow cell comprising a flow cell body, a laminar liquid flow channel arranged in the flow cell body, a sheath liquid inlet arranged on the flow cell body and connected to the laminar liquid flow channel, and a liquid outlet arranged on the flow cell body and connected to one end of the laminar liquid flow channel, the laminar liquid flow channel is connected to the sample nozzle and the sheath liquid inlet, the sheath liquid inlet and the sample nozzle are arranged at the same end of the laminar liquid flow channel, the profile of the laminar liquid flow channel is a tapered arc in a cross section along the extension direction of the laminar liquid flow channel, and the profile of the laminar liquid flow channel is a tapered arc in a cross section perpendicular to the extension direction of the laminar liquid flow channel. On the surface, the outline of the laminar liquid flow channel is elliptical, the sample nozzle includes a plug-in tube, a nozzle base body arranged at one end of the plug-in tube, a sample inlet arranged on the nozzle base body and connected to the plug-in tube, and a flat nozzle arranged on the end of the plug-in tube away from the nozzle base body, the sheath liquid inlet and the sample nozzle are both arranged at the same end of the laminar liquid flow channel, the flat nozzle and the long axis of the laminar liquid flow channel are parallel to each other, the position of the sheath liquid nozzle in the direction of sample flow is higher than the position of the flat nozzle, the flow rate of the sheath liquid under the drive of the injection mechanism is between 128uL / s-146uL / s and the flow rate ratio between the sheath liquid and the sample liquid is between 31.4-35.8 to stratify the sample, and after the sample is stratified, the imaging module images the sample to obtain a clear image.

[0012] Furthermore, the frame includes a syringe mounting frame and four syringe plates arranged on the syringe mounting frame.

[0013] Furthermore, the syringe mounting bracket is in an "L" shape as a whole, and its short side portion is the solenoid valve mounting portion.

[0014] Furthermore, each of the injector plates includes an injector plate body, a sliding hole arranged on one end of the injector plate body away from the solenoid valve mounting portion, and a bottom plate groove arranged on the end of the injector plate body close to the sliding hole.

[0015] Further, the injection mechanism includes a solenoid valve unit arranged on the solenoid valve mounting portion, a syringe unit arranged on the syringe plate, and four motor assemblies arranged on the end surface of the syringe plate facing away from the syringe unit. The injection mechanism includes a solenoid valve unit arranged on the solenoid valve mounting portion, a syringe unit arranged on the syringe mounting plate, and four motor assemblies arranged on the end surface of the syringe mounting plate facing away from the syringe unit.

[0016] Further, the solenoid valve unit includes a first solenoid valve, a second solenoid valve, a third solenoid valve, a fourth solenoid valve, a fifth solenoid valve, a sixth solenoid valve, a seventh solenoid valve, and an eighth solenoid valve arranged in sequence from left to right.

[0017] Furthermore, the syringe unit includes a cleaning pump assembly, a sheath pump assembly, a sample flow pump assembly, a sample suction pump assembly, and a sample suction needle connected to the sample suction pump assembly and the first solenoid valve, which are arranged on the frame from right to left.

[0018] Furthermore, the cleaning pump assembly, the sheath fluid pump assembly, the sample flow pump assembly, and the sample suction pump assembly all have the same structure, and are all composed of a syringe body and a push rod disposed on the end of the syringe body away from the solenoid valve.

[0019] Furthermore, each of the motor assemblies includes a motor mounting plate on the frame, a base plate arranged at one end of the frame, a linear motor fixedly arranged on the motor mounting plate, at least two guide shafts whose ends are respectively arranged on the linear motor and the base plate, and a slider whose one end is connected to the movable end of the linear motor and is movably arranged on the guide shaft.

[0020] Furthermore, an exhaust port is provided at one end of the laminar flow channel close to the sheath liquid inlet.

[0021] Furthermore, a breathable and waterproof membrane is provided at the exhaust port.

[0022] Compared with the prior art, the present invention provides a formed component imaging system that draws the sample into the sample laminar flow pump assembly by setting the sample suction needle. The solenoid valve unit is connected to the syringe unit, so that the running speed of the sample laminar flow pump assembly and the sheath liquid pump assembly can be accurately controlled by the motor assembly after computational fluid dynamics, thereby controlling the sample flow rate and the sheath liquid flow rate, so that the sample can form a stable laminar flow effect in the flow channel to avoid the superposition of formed components. And the flow pool is made of colorless high-transmittance glass in one piece, so that it has high light transmittance and high sealing effects, avoiding the impact of airtightness and light transmittance problems on imaging. And the thickness of the laminar flow is limited to 4±0.5 microns, which is much smaller than the 8-micron diameter of the formed component, so that the characteristic surface of the formed component can face the camera and pass through the flow channel with the laminar flow, ensuring that the imaging module can capture the formed components flowing with the laminar flow. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention provides a structural schematic diagram of a formed component imaging system.

[0024] Figure 2 for Figure 1 Schematic diagram of the structure of the formed component imaging system from another perspective.

[0025] Figure 3 for Figure 1 Schematic diagram of the structure of the formed ingredient imaging system having an injector plate.

[0026] Figure 4 for Figure 1 A formed component imaging system has a structural schematic diagram of an injector unit.

[0027] Figure 5 for Figure 1 Schematic diagram of the structure of the flow cell of the formed element imaging system.

[0028] Figure 6 for Figure 5 Schematic diagram of the cross-sectional structure of the flow cell.

[0029] Figure 7 for Figure 6 A schematic diagram of the cross-sectional structure of the flow cell from another perspective. DETAILED DESCRIPTION

[0030] The specific embodiments of the present invention are further described in detail below. It should be understood that the description of the embodiments of the present invention herein is not intended to limit the protection scope of the present invention.

[0031] like Figures 1 to 7As shown, it is a schematic diagram of the structure of a formed component imaging system provided by the present invention. The formed component imaging system includes a liquid injection mechanism 20, a detection mechanism 30, and a flow cell mechanism 40 arranged in the detection mechanism 30. It can be imagined that the formed component imaging system also includes some other functional structures, such as pipeline integration, screw slide, etc., which are well known to those skilled in the art and will not be repeated here.

[0032] The frame 10 is fixedly mounted on a workbench (not shown) to provide support for the above-mentioned functional modules. The frame 10 includes a syringe mounting frame 11, and four syringe plates 12 arranged on the syringe mounting frame 11. The syringe mounting frame 11 is generally "L"-shaped, and its short side portion is a solenoid valve mounting portion 13, which provides space for installing the solenoid valve. The four syringe plates 12 are arranged on the syringe mounting frame 11. Each of the syringe plates 12 includes a syringe plate body 121, a sliding hole 122 arranged on one end of the syringe plate body 121 away from the solenoid valve mounting portion 13, and a bottom plate groove 123 arranged on the end of the syringe plate body 121 close to the sliding hole 122. The syringe plate 12 is used in conjunction with the injection mechanism 20, which will be described in detail below in conjunction with the injection mechanism 20.

[0033] The injection mechanism 20 includes a solenoid valve unit 21 disposed on the solenoid valve mounting portion 13 , a syringe unit 22 disposed on the syringe plate 12 , and four motor assemblies 23 disposed on the end surface of the syringe plate 12 facing away from the syringe unit 22 .

[0034] See also Figure 1 The solenoid valve unit 21 includes a first solenoid valve 211, a second solenoid valve 212, a third solenoid valve 213, a fourth solenoid valve 214, a fifth solenoid valve 215, a sixth solenoid valve 216, a seventh solenoid valve 217, and an eighth solenoid valve 218, which are arranged from left to right. The first solenoid valve 211 is connected to the second solenoid valve 212 through a pipeline. The number of the solenoid valves can be increased or decreased according to actual needs.

[0035] The solenoid valve unit 21 is connected to the syringe unit 22. The syringe unit 22 includes a cleaning pump assembly 221, a sheath pump assembly 222, a sample laminar flow pump assembly 223, a sample suction pump assembly 224, and a sample suction needle 227 connected to the sample suction pump assembly 224 and the first solenoid valve 211, which are arranged on the syringe plate 12 from right to left. The first solenoid valve 211 and the second solenoid valve 212 are connected to the sample suction pump assembly 224 and the sample laminar flow pump assembly 223, so that the sample suction pump assembly 224 and the sample laminar flow pump assembly 223 can close one of the pipelines separately, which is convenient for controlling the flow direction of the liquid. The connecting pipeline between the first solenoid valve 211 and the sample suction pump assembly 224 is also connected to the sample suction needle 227, so that the sample suction pump assembly 224 can absorb the sample through the sample suction needle 227 and transport it to the sample laminar flow pump assembly 223. The cleaning pump assembly 221 and the third solenoid valve 213, the fifth solenoid valve 215, the sixth solenoid valve 216, the seventh solenoid valve 217, and the eighth solenoid valve 218 are all connected to the pipeline integration system (not shown) to achieve the purpose of conveying different types of sample liquids, such as urine, blood, etc., to avoid the contamination of samples caused by multiple sample liquids sharing one solenoid valve, and to clean the flow cell. Since this embodiment mainly describes the absorption of samples and the formation of laminar flow with sheath liquid, the third solenoid valve 213, the fifth solenoid valve 215, the sixth solenoid valve 216, the seventh solenoid valve 217, and the eighth solenoid valve 218 are not described separately.

[0036] The cleaning pump assembly 221, the sheath liquid pump assembly 222, the sample laminar flow pump assembly 223, and the sample suction pump assembly 224 are all of the same structure, and are all composed of a syringe body 225 and a push rod 226 arranged at the end of the syringe body 225 away from the electromagnetic valve unit 21. The electromagnetic valve unit 21 and the syringe body 225 are connected by a pipeline. The cleaning pump assembly 221 is used to pump cleaning liquid into the detection mechanism 30 for cleaning. The sheath liquid pump assembly 222 is used to pump sheath liquid into the detection mechanism 30, and the flow rate of the sheath liquid in the sheath liquid pump assembly 222 is adjusted by the fourth electromagnetic valve 214 and the motor assembly 23. The sample laminar flow pump assembly 223 is used to pump sample liquid into the detection mechanism 30, and the flow rate of the sample in the sample laminar flow pump assembly 223 is adjusted by the second electromagnetic valve 212 and the motor assembly 23. The sample suction pump assembly 224 is used for suctioning samples. The above four components all need to be used in conjunction with the motor assembly 23.

[0037] The number of the motor assemblies 23 corresponds to the number of assemblies included in the injector unit 22. Each of the motor assemblies 23 includes a motor mounting plate 231 on the frame 10, a bottom plate 232 disposed at one end of the frame 10, a linear motor 233 fixedly disposed on the motor mounting plate 231, at least two guide shafts 234 having two ends disposed on the linear motor 233 and the bottom plate 232, respectively, and a slider 235 having one end connected to a movable end of the linear motor 233 and movably disposed on the guide shaft 234.

[0038] The movable end of the linear motor 233 is arranged toward the bottom plate 232, so as to drive the slider 235 to slide along the guide shaft 234. Two linear bearings 236 are arranged in the middle of the guide shaft 234. Both the two linear bearings 236 protrude from the two end surfaces of the slider 235. The linear bearings 236 are prior art and are not described in detail here. The slider 235 is slidably arranged on the guide shaft 234 through the two linear bearings 236, and the self-lubricating effect of the linear bearings 236 reduces wear and improves sliding smoothness. Each slider 235 is connected to a push rod 226, and the push rod 226 is pushed by the linear motor 233 to push out the sample in the syringe body 225. In addition, the operator can control the pushing speed of the push rod 226 by adjusting the speed of the linear motor 233 to adjust the flow speed of the sample and the sheath fluid.

[0039] When the syringe unit 22 and the motor assembly 23 are in use, after fluid mechanics calculations, it is first ensured that the "Reynolds coefficient (fluid mechanics term)" of the sheath liquid and the sample is less than 2300, so that a laminar flow is formed between the sheath liquid and the sample liquid. Then the speed of the two linear motors 233 is adjusted to change the flow rate of the sheath liquid and the sample liquid, and then change the thickness of the laminar flow, that is, a faster flow rate will make the laminar flow thinner, and a slower flow rate will make the laminar flow thicker. If the sheath liquid flow rate is too fast, the thickness of the laminar flow will be too thin, and more light sources will penetrate the flow cell assembly 40 and then irradiate the imaging module 34, which is not conducive to focusing. If the sheath liquid flow rate is too slow, the laminar flow will become too thick, and the light source cannot completely penetrate the sheath liquid, so that the light diffuses in the sheath liquid, which is not conducive to the imaging module 34 to shoot the visible components in the sample liquid.

[0040] Therefore, it is necessary to control the flow rate between the sheath liquid and the sample liquid. The flow rate range of the sheath liquid is between 128uL / s and 146uL / s. The reason for this range is that only within this range can the sheath liquid flow down along the side wall of the laminar flow channel 412 described below to wrap the sample. When it is less than 128uL / s, it will gather into clumps due to the effect of surface tension and cannot form a layer. When it is greater than 146uL / s, it will not be able to hang on the wall due to the too fast flow rate, thus forming a linear flow, and it is also impossible to wrap the sample below. Specifically, when the sheath liquid flow rate is 128uL / s and the sample flow rate is 4.08uL / s, the laminar thickness is 4 microns. When the sample flow rate remains unchanged, the sheath liquid flow rate is 114uL / s and the laminar thickness is 4.5 microns. When the sheath liquid flow rate is 146uL / s, the laminar thickness is 3.5 microns. Therefore, in this embodiment, the flow rate of the sample liquid is maintained at 4.08uL / s, and the flow rate of the sheath liquid under the driving of the injection mechanism 20 is between 128uL / s and 146uL / s, and the flow rate ratio between the sheath liquid and the sample liquid is between 31.4 and 35.8 to stratify the sample, that is, the flow rate of the sheath liquid is between 31.4 and 35.8 times that of the sample liquid. It can be imagined that while maintaining the flow rate ratio, the flow rates of the sheath liquid and the sample liquid can be scaled in proportion to each other to adapt to devices with different sampling efficiencies.

[0041] The detection mechanism 30 includes a substrate 31, a flow pool holder 32 arranged in the middle area of ​​the substrate 31, an illumination module 33 arranged on the substrate 31 and located on one side of the flow pool holder 32, and an imaging module 34 arranged on the substrate 31 and located on the other side of the flow pool holder 32.

[0042] The substrate 31 is fixed on a flat surface such as a desktop or a workbench, and is used to carry the above-mentioned functional modules, such as the flow cell support 32, the lighting module 33, and the imaging module.

[0043] The flow pool bracket 32 ​​is used to clamp and fix the flow pool assembly 40, and can also adjust the position of the flow pool assembly 40. The flow pool bracket 32 ​​includes a bracket base 321, a bracket 322 arranged on the bracket base 321, a clamping base 323 embedded in the bracket 322, and a clamping cover 324 covering the clamping base 323. The bracket 322 is vertically arranged on the bracket base 321. The clamping base 323 and the clamping cover 324 are arranged on the end face of the bracket 322 facing away from the lighting module 33. A lighting hole 325 is opened on the bracket 322 at a position corresponding to the lighting module 33, so that the light source of the lighting module 33 can directly irradiate the flow pool bracket 32.

[0044] The lighting module 33 is a device currently available on the market that provides a stable light source for sample detection, such as a laser or a flash xenon lamp, etc. It is a prior art and will not be described in detail here.

[0045] The imaging module 34 is used to capture images of formed components in the sample under the light source for analysis. The imaging module 34 is made of heat-insulating material to prevent the optical unit from being affected by temperature.

[0046] The flow cell assembly 40 includes a flow cell 41, a sample nozzle 42 disposed on the flow cell 41, and a sheath fluid nozzle 43 disposed on the flow cell 41. The flow cell assembly 40 is placed in the clamping base 323, and the clamping cover 324 is covered on the flow cell assembly 40 and screwed to the clamping base 323, so as to fix the flow cell assembly 40 in the flow cell bracket 32.

[0047] The flow cell 41 includes a flow cell body 411, a laminar flow channel 412 disposed in the flow cell body 411, a sheath liquid inlet 413 disposed on the flow cell body 411 and connected to the laminar flow channel, and a liquid outlet 414 disposed on the flow cell body 411 and connected to one end of the laminar flow channel 412. The flow cell 41 itself is a prior art, such as a test flow cell for urine analysis disclosed in CN118190928A.

[0048] The flow cell body 411 is made of colorless high-transmittance glass and is made by a fusion-integrated processing method. The use of colorless high-transmittance glass allows light to pass through almost without loss, ensuring the clarity of imaging. The fusion-integrated processing method can also ensure the integrity and sealing of the structure, reduce the risk of leakage, and ensure the uniformity of the material, reducing the imaging error caused by material unevenness.

[0049] An exhaust port 415 is further disposed at one end of the laminar flow channel 412 close to the sheath liquid inlet 413 .

[0050] The laminar liquid flow channel 412 is used to guide the flow of the test sample. The laminar liquid flow channel 412 is connected to the sample nozzle 42, the sheath liquid inlet 413, and the exhaust port 415. The sheath liquid inlet 413 and the sample nozzle 42 are arranged at the same end of the laminar liquid flow channel 412. The sample nozzle 42 injects the sample liquid into the laminar liquid flow channel 412, the sheath liquid inlet 413 injects the sheath liquid into the laminar liquid flow channel 412, and the exhaust port 415 discharges the excess bubbles in the laminar liquid flow channel 412. On the cross section along the extension direction of the laminar liquid flow channel 412, the profile of the laminar liquid flow channel 412 is a tapered arc. On the cross section perpendicular to the extension direction of the laminar liquid flow channel 412, the profile of the laminar liquid flow channel 412 is an ellipse.

[0051] The sheath liquid inlet 413, the exhaust port 415, and the sample nozzle 42 are all arranged at the same end of the laminar liquid flow channel 412. A breathable and waterproof membrane is arranged at the exhaust port 415 to prevent liquid leakage. The liquid outlet 414 is arranged at the end of the laminar liquid flow channel 412 facing away from the sample nozzle 42 to realize the entry and exit of the sample liquid sample.

[0052] The sample nozzle 42 is used to inject the sample liquid sample into the laminar liquid flow channel 412, and the sample nozzle 42 includes a plug-in tube 421, a nozzle base body 422 arranged at one end of the plug-in tube 421, a sample liquid inlet 423 arranged on the nozzle base body 422 and connected to the plug-in tube 421, and a flat nozzle 424 arranged on the end of the plug-in tube 421 away from the nozzle base body 422.

[0053] When the sample nozzle 42 is fixed on the flow cell body 411, the insertion tube 421 is inserted into the laminar flow channel 412. The nozzle base body 422 is fixed and sealed between the flow cell body 411 by glue.

[0054] See also Figure 7, the flat nozzle 424 makes the sample liquid appear flat when it is sprayed into the laminar liquid flow channel 412, and the flat nozzle 424 is located at the center of the laminar liquid flow channel 412. The width of the laminar liquid flow channel 412 containing the flat nozzle 424 is greater than the width of the other end of the laminar liquid flow channel 412, so that a tapered section 416 is formed in the middle area of ​​the laminar liquid flow channel 412. The tapered section 416 can guide the flow direction of the sheath liquid, and the tapered section 416 is directly opposite to the flat nozzle 424, so that the sample liquid can be wrapped by the sheath liquid flowing down from the two side walls of the tapered section 416 after being sprayed out. The long axes of the flat nozzle 424 and the laminar liquid flow channel 412 are parallel to each other, so that the sample liquid flowing out of the flat nozzle 424 can directly flow into the space between the two side walls of the tapered section 416.

[0055] One end of the sheath liquid nozzle 43 is disposed in the sheath liquid inlet 413. When the flow cell 41 is clamped on the flow cell bracket 32, the position of the liquid outlet of the sheath liquid nozzle 43 is higher than the position of the liquid outlet of the flat nozzle 424 in the direction of sample flow, so that the sheath liquid can wrap the sample liquid when flowing in the laminar liquid flow channel 412, that is, the sample liquid can be immediately wrapped by the sheath liquid after flowing out of the flat nozzle 424, thereby forming a laminar flow earlier, which increases the time for the subsequent shooting of the imaging module 34.

[0056] The sheath liquid inlet 413 is connected to the second electromagnetic valve 212 , and the sample nozzle 42 is connected to the fourth electromagnetic valve 214 through a pipeline, so as to control the flow of the sample and the sheath liquid in the laminar liquid flow channel 412 .

[0057] The sheath fluid mentioned above is a balanced electrolyte solution with no fluorescence background, which is mainly used in flow cytometers and some blood analyzers to improve the accuracy of detection.

[0058] When in use, the sample laminar flow pump assembly 223 is connected to the sample nozzle 42, and the sheath liquid pump assembly 222 is connected to the sheath liquid inlet 413. The two motor assemblies 23 respectively push the sample laminar flow pump assembly 223 and the sheath liquid pump assembly 222 to inject the sample and the sheath liquid into the laminar flow channel 412. At the same time, after the calculation of fluid mechanics, the second solenoid valve 212 and the fourth solenoid valve 214 respectively control the sample laminar flow pump assembly 223 and the sheath liquid pump assembly 222, so as to control the sample flow rate and the sheath liquid flow rate, and at the same time, the sheath liquid flow rate is greater than the sample flow rate, and flows down from the two side walls of the tapered section 416 to ensure that the sheath liquid can surround the sample liquid, so that the sample forms a stable laminar flow effect in the laminar flow channel 412, that is, the sample liquid is in the center, the laminar thickness is 4±0.5 microns, and the sheath liquid is wrapped on both sides.

[0059] Because the sample cells flow out of the sample nozzle 42 continuously or even overlap, in order to allow the camera to capture individual sample cells one by one, the continuous or even overlapping sample cells must be separated. However, it cannot be too fast, otherwise the cells will be broken or the imaging module 34 will be difficult to focus.

[0060] Therefore, the ratio of the speed of the sheath liquid to the speed of the sample must be controlled within a certain range, so that the sheath liquid with a fast flow rate can bring out the independent sample cells in the sample with a slow flow rate one by one and have a certain interval between each other, so as to achieve the effect of stratification, and when flowing through the laminar flow channel 412, the imaging module 34 takes pictures. Therefore, when the flow rate of the sheath liquid is between 128uL / s-146uL / s, the flow rate ratio between the sheath liquid and the sample liquid is between 31.4-35.8, that is, the flow rate of the sheath liquid is between 31.4 and 35.8 times that of the sample liquid. If the flow rate of the sheath liquid is less than 128uL / s and the flow rate ratio is less than 31.4, it will not be possible to separate the sample cells from the sample flow one by one, and if the flow rate of the sheath liquid is greater than 146uL / s and the flow rate ratio is less than 35.8, it may break through the sample cells and the imaging cannot be accurately focused.

[0061] And because the diameters of the formed elements in the sample (such as red blood cells, white blood cells, epithelial cells, etc.) are all above 8 microns, when the laminar flow thickness is smaller than the diameter of the formed elements, the characteristic surface of the formed elements can be directed toward the camera through the flow cell channel, thereby enabling the imaging module 34 to capture higher-definition images for analysis.

[0062] Compared with the prior art, the imaging system of a formed component provided by the present invention absorbs the sample into the sample laminar flow pump assembly 223 by setting the sample suction needle 227. The electromagnetic valve unit 21 is connected to the syringe unit 22, so that the running speed of the sample laminar flow pump assembly 223 and the sheath liquid pump assembly 222 can be accurately controlled by the motor assembly 23 after computational fluid dynamics, thereby controlling the sample flow rate and the sheath liquid flow rate, so that the sample can form a stable laminar flow effect in the laminar flow channel 412 to avoid the superposition of formed components. And the flow pool 41 is formed of colorless high-transmittance glass in one piece, so that it has high light transmittance and high sealing effect, avoiding the airtightness and light transmittance problems affecting the imaging. And the thickness of the laminar flow is limited to 4±0.5 microns, which is much smaller than the 8-micron diameter of the formed component, so that the characteristic surface of the formed component can face the camera and pass through the laminar flow channel 412 with the laminar flow, ensuring that the imaging module 34 can capture the formed components flowing with the laminar flow.

[0063] The above are only preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modification, equivalent substitution or improvement within the spirit of the present invention is included in the scope of the claims of the present invention.

Claims

1. A shaped component imaging system, characterized in that: The formed component imaging system includes a liquid injection mechanism, a detection mechanism, and a flow cell assembly arranged in the detection mechanism, the detection mechanism includes an imaging module, the flow cell assembly includes a flow cell, and a sample nozzle arranged on the flow cell, the flow cell includes a flow cell body, a laminar liquid flow channel arranged in the flow cell body, a sheath liquid inlet arranged on the flow cell body and connected to the laminar liquid flow channel, and a liquid outlet arranged on the flow cell body and connected to one end of the laminar liquid flow channel, the laminar liquid flow channel is connected to the sample nozzle and the sheath liquid inlet, the sheath liquid inlet and the sample nozzle are arranged at the same end of the laminar liquid flow channel, in the cross section along the extension direction of the laminar liquid flow channel, the contour of the laminar liquid flow channel is a tapered arc, and in the cross section perpendicular to the extension direction of the laminar liquid flow channel The laminar liquid flow channel has an elliptical outline, and the sample nozzle includes a plug-in tube, a nozzle base body arranged at one end of the plug-in tube, a sample liquid inlet arranged on the nozzle base body and connected to the plug-in tube, and a flat nozzle arranged on the end of the plug-in tube away from the nozzle base body. The sheath liquid inlet and the sample nozzle are both arranged at the same end of the laminar liquid flow channel, and the flat nozzle is parallel to the long axis of the laminar liquid flow channel. In the direction of sample flow, the position of the sheath liquid nozzle outlet is higher than the position of the flat nozzle outlet. Under the drive of the injection mechanism, the flow rate of the sheath liquid ranges from 128uL / s to 146uL / s, and the flow rate ratio between the sheath liquid and the sample liquid is between 31.4-35.8 to stratify the sample. After the sample is stratified, the imaging module images the sample to obtain a clear image.

2. The shaped component imaging system according to claim 1, characterized in that: The frame includes a syringe mounting frame and four syringe plates arranged on the syringe mounting frame.

3. The shaped component imaging system according to claim 2, characterized in that: The syringe mounting frame is in an "L" shape as a whole, and its short side portion is the solenoid valve mounting portion.

4. The shaped component imaging system according to claim 3, characterized in that: Each of the injector plates includes an injector plate body, a sliding hole arranged on one end of the injector plate body away from the solenoid valve mounting portion, and a bottom plate groove arranged on the end of the injector plate body close to the sliding hole.

5. The shaped component imaging system according to claim 1, characterized in that: The injection mechanism includes a solenoid valve unit arranged on the solenoid valve mounting portion, a syringe unit arranged on the syringe plate, and four motor assemblies arranged on the end surface of the syringe plate facing away from the syringe unit. The solenoid valve unit includes a first solenoid valve, a second solenoid valve, a third solenoid valve, a fourth solenoid valve, a fifth solenoid valve, a sixth solenoid valve, a seventh solenoid valve, and an eighth solenoid valve arranged in sequence from left to right.

6. The shaped component imaging system according to claim 5, characterized in that: The syringe unit includes a cleaning pump assembly, a sheath pump assembly, a sample flow pump assembly, a sample suction pump assembly, and a sample suction needle connected to the sample suction pump assembly and the first solenoid valve, which are arranged on the frame from right to left.

7. The shaped component imaging system according to claim 6, characterized in that: The cleaning pump assembly, the sheath fluid pump assembly, the sample flow pump assembly, and the sample suction pump assembly all have the same structure, and are all composed of a syringe body and a push rod arranged on the end of the syringe body away from the solenoid valve.

8. The shaped component imaging system according to claim 5, characterized in that: Each of the motor assemblies includes a motor mounting plate on the frame, a base plate arranged at one end of the frame, a linear motor fixedly arranged on the motor mounting plate, at least two guide shafts whose ends are respectively arranged on the linear motor and the base plate, and a slider with one end connected to the movable end of the linear motor and movably arranged on the guide shaft.

9. The shaped component imaging system according to claim 1, characterized in that: An exhaust port is also provided at one end of the laminar flow channel close to the sheath liquid inlet.

10. The shaped component imaging system according to claim 9, characterized in that: A breathable and waterproof membrane is arranged at the exhaust port.

Images