Linkage control system for optical adapters of incident light and emergent light

Abstract

The invention discloses an incident light and emergent light optical adapter linkage control system, and the system comprises a display device which is provided with an incident light selection module and an emergent light output selection module; the incident light selection module is used for receiving the selected incident light source; the emergent light output selection module is used for receiving a selected optical component to be guided in; the control equipment is used for receiving a signal sent by the display equipment and transmitting different control signals to the incident light optical adapter and the emergent light optical adapter based on the selected incident light source and the optical component to be guided in; the incident light optical adapter is used for receiving the control signal sent by the control equipment, controlling the selected incident light source to be incident and transmitting the corresponding incident light source to the sample; and the emergent light optical adapter is used for receiving the control signal sent by the control equipment, controlling the entrance of the emergent signal of the collected sample, and transmitting the emergent signal to the optical assembly to be guided in. According to the invention, the operation is simple and convenient, and linkage adjustment of the optical adapter is fast and accurate.

Description

Technical Field

[0001] The invention relates to the field of optical adapters of deep ultraviolet multi-dimensional laser confocal microfluorescence spectroscopy systems (DUV-MD-LCMFSA), and in particular to a linkage control system of incident light and outgoing light optical adapters. Background Art

[0002] The Deep UV and multi-dimension laser confocal scanning microscopic fluorescence spectral instrument (DUV-MD-LCMFSA) is a scientific research equipment independently developed by my country, which can promote my country's leading position in the research of organic pollutant environmental micro-interfaces and micro-fluorescence spectroscopy technology. The Deep UV and multi-dimension laser confocal scanning microscopic fluorescence spectral system realizes the combination of laser confocal microscopy with fluorescence lifetime imaging technology and high-sensitivity fluorescence spectroscopy technology, which can minimize the interference of background and non-target fluorescence signals in the environment and improve the sensitivity and selectivity of organic pollutant detection in micro-regions and micro-interfaces. At the same time, it has the ability to examine fluorescent substances in the deep ultraviolet region. It is a high-resolution microscopic imaging method that realizes spectral imaging of experimental samples through laser excitation of samples, fluorescence collection and computer image processing. Among them, the optical adapter is an important component, which is used to control the optical path of the incident light and the outgoing light to realize micro-region microscopy, lifetime imaging and spectral quantitative analysis of experimental samples.

[0003] When a traditional laser confocal microscope is coupled with other functional components, the optical adapter needs to be adjusted manually, which is cumbersome and error-prone, affecting the imaging quality, efficiency and even the life of the instrument. In recent years, with the development of computer technology and automation technology, some automated optical adapter control systems have emerged. However, the existing control systems have problems such as low control accuracy, complex operation, and incompatibility, making it difficult to obtain information about the research object. Summary of the invention

[0004] The purpose of the present invention is to address the defects and shortcomings in the prior art and propose a linkage control system for incident light and outgoing light optical adapters, which can realize the software and hardware linkage control of incident light source switching and outgoing signal introduction into different optical components, has the advantages of simple operation, fast and accurate, precise automation, etc., can realize efficient linkage and coupling of the functions of optical components of deep ultraviolet multi-dimensional laser confocal microscopy fluorescence spectroscopy system, improve imaging quality and efficiency, and has broad application prospects.

[0005] The present invention adopts the following technical solution:

[0006] An incident light and an outgoing light optical adapter linkage control system, comprising: a display device, a control device, an incident light optical adapter and an outgoing light optical adapter; the control device is connected to the display device, the incident light optical adapter and the outgoing light optical adapter respectively;

[0007] The display device is provided with an incident light selection module and an outgoing light output selection module; the incident light selection module is used to receive a selected incident light source; the outgoing light output selection module is used to receive an optical component into which a selected outgoing signal is to be introduced;

[0008] The control device receives the signal sent by the display device, and transmits different control signals to the incident light optical adapter and the outgoing light optical adapter based on the selected incident light source and the optical component to be introduced;

[0009] The incident light optical adapter receives a control signal sent by the control device, controls the selected incident light source to be incident, and transmits the corresponding incident light source to the sample;

[0010] The output light optical adapter receives the control signal sent by the control device, controls the output signal of the collected sample to enter, and transmits the output signal to the optical component to be introduced.

[0011] Preferably, the display device is further provided with a communication port channel selection module for selecting a required communication port channel to perform optical path setting.

[0012] Preferably, the display device is further provided with a path selection module for controlling whether to allow selection of the incident light selection module and the outgoing light output selection module.

[0013] Preferably, the incident light source selected by the incident light selection module includes a steady-state light source or a pulse light source.

[0014] Preferably, the incident light selection module is further used to receive a first emergency stop instruction to stop switching of the incident light source.

[0015] Preferably, the output light selection module receives the selected optical component to be introduced, including a fluorescence lifetime component or a fluorescence microscopy imaging component.

[0016] Preferably, the output light selection module is further used to receive a second emergency stop instruction to stop the switching of the optical component to be introduced.

[0017] Preferably, the incident light optical adapter and the output light optical adapter have the same structure, including: a motor, an actuator, a sliding platform with a built-in reflector and a magnetic barrier; the motor receives control information sent by a control device, drives the actuator to drive the sliding platform to move; the magnetic barrier adsorbs the sliding platform when the sliding platform is close; the reflector is used to change the direction of the incident light path.

[0018] Preferably, the process of the control device controlling the incident light optical adapter to select the incident light source is as follows:

[0019] When the selected incident light source is a pulse light source, the control device sends a signal to the motor to control the sliding platform to be located on the far left, and the pulse light source enters from the first interface of the incident light optical adapter and is output to the sample detection component through the second interface of the incident light optical adapter; when the selected incident light source is a steady-state light source, the control device sends a signal to the motor to control the motor to drive the execution component to push the sliding platform to the far right until it is adsorbed by the magnetic blocker, and the steady-state light source enters from the third interface of the incident light optical adapter, changes direction after passing through the reflector, and is output to the sample detection component from the second interface.

[0020] Preferably, the process in which the control device controls the outgoing light optical adapter to transmit the outgoing signal to the optical component to be introduced is as follows:

[0021] When the optical component selected to be introduced is a fluorescence lifetime component, the control device sends a signal to the motor to control the sliding platform to be located at the far left, and the output signal output by the sample detection component enters from the second interface of the output light optical adapter, and is output to the fluorescence lifetime component through the first interface of the output light optical adapter; when the optical component selected to be introduced is a fluorescence microscopy imaging component, the control device sends a signal to the motor to control the motor to drive the execution component to push the sliding platform to the far right until it is adsorbed by the magnetic blocker, and the output signal output by the sample detection component enters from the second interface, changes direction after passing through the reflector, and is output from the third interface of the output light optical adapter to the fluorescence microscopy imaging component.

[0022] It can be seen from the above description of the present invention that, compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The present invention is provided with a communication port channel selection module (COM Set), an incident light selection module (Input Laser), an output light output selection module (Output Laser) and a path selection module (Open port) on the display device, which can realize the visualization and numerical control operation of the optical path switching state, is easy to operate, and is faster and more accurate than manual switching;

[0024] (2) The present invention controls the motor of the optical adapter through a control device, thereby realizing software-controlled optical path switching and output light introduction into different functional components without disassembling the device, which greatly facilitates laboratory operation and sample detection of deep ultraviolet multi-dimensional laser confocal microscopy fluorescence spectroscopy system. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 It is a structural block diagram of the incident light and outgoing light optical adapter linkage control system according to an embodiment of the present invention;

[0027] Figure 2 A schematic diagram of each module on a display device according to an embodiment of the present invention;

[0028] Figure 3 A schematic diagram of selecting an incident light selection module according to an embodiment of the present invention;

[0029] Figure 4 A schematic diagram of selecting an output light selection module according to an embodiment of the present invention;

[0030] Figure 5 is a schematic structural diagram of an optical adapter according to an embodiment of the present invention;

[0031] Figure 6 Schematic diagram of the direction selection of the incident light source in the incident light optical adapter according to an embodiment of the present invention; wherein (a) indicates that the incident light source is a pulse light source; (b) indicates that the incident light source is a steady-state light source;

[0032] Figure 7 Schematic diagram of the destination selection of the output signal in the output light optical adapter of an embodiment of the present invention; wherein (a) indicates that the optical component to be introduced is a fluorescence lifetime component; (b) indicates that the optical component to be introduced is a fluorescence lifetime component fluorescence microscopy imaging component. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] See also Figure 1 As shown, a linkage control system of incident light and incident light optical adapter includes: a display device 1, a control device 2, an incident light optical adapter 3 and an incident light optical adapter 4; the control device 2 is connected to the display device 1, the incident light optical adapter 3 and the incident light optical adapter 4 respectively;

[0035] The display device 1 is provided with an incident light selection module and an outgoing light output selection module; the incident light selection module is used to receive a selected incident light source; the outgoing light output selection module is used to receive an optical component into which a selected outgoing signal is to be introduced;

[0036] The control device 2 receives the signal sent by the display device 1, and transmits different control signals to the incident light optical adapter 3 and the incident light optical adapter 4 based on the selected incident light source and the optical component to be introduced;

[0037] The incident light optical adapter 3 receives the control signal sent by the control device 2, controls the selected incident light source to be incident, and transmits the corresponding incident light source to the sample;

[0038] The incident light optical adapter 4 receives the control signal sent by the control device 2, controls the output signal of the collected sample to enter, and transmits the output signal to the optical component to be introduced.

[0039] Specifically, the display device 1 is further provided with a communication port channel selection module for selecting a required communication port channel to perform optical path setting.

[0040] The display device 1 is further provided with a path selection module for controlling whether to allow the incident light selection module and the outgoing light output selection module to be selected.

[0041] In this embodiment, the display device 1 (such as a computer) and the control device 2 (such as a controller) are connected via a USB data cable. The control device 2 (such as a controller) is connected to the incident light optical adapter 3 and the control end of the incident light optical adapter 4 via an optical fiber data cable. The line coming out of the control device 2 (such as a controller) is split into two at the rear end, one connected to the incident light optical adapter 3, and the other connected to the incident light optical adapter 4.

[0042] See also Figure 2The figure shows the operation interface of the display device 1 when the path selection module is not opened, that is, Open Port is not clicked, and the communication port channel selection module is available. The user can set the parameters under COM Set, including: COM Port (serial communication port), Parity (parity check), Stop Bits (stop bits), Baud Rate (baud rate), Data Bits (data bits).

[0043] In this embodiment, when adjusting the optical path, you can click COM Port to open the serial communication port and select the communication port channel, click Parity and select "None", click Stop Bits and select "One", click Baud Rate and select "19200", click Data Bits and select "8", and complete the optical path setting.

[0044] Then click Open port and follow the experimental requirements. Figure 3 The “Input laser” shown in the figure sets the incident light source. This embodiment has two types of light sources, one is a steady-state laser 5 (CW Laser) and the other is a pulse light source 6 (Pulse Laser). Different light source types can be selected according to the experimental purpose. In addition, Figure 3 In the “Input laser”, you can also select Stop to protect the device by emergency stopping the incident light path switching action when an error occurs during the switching of the incident light path.

[0045] Depending on the purpose of the experiment, the optical information collected from the sample needs to be transmitted to different detection instruments. This process is the process of selecting the direction of the emitted light. Figure 4 The selection is achieved by setting the "Output laser" shown in the figure. In this embodiment, there are two detection instruments, one is to obtain the conventional fluorescence microscopy imaging component 8 "FS", and the other is the fluorescence lifetime component 7 "FLIM". In addition, Figure 4 In the output laser, you can also select Stop under "Output laser" to stop the output light path switching action urgently when an error occurs during the switching process to protect the equipment.

[0046] After the settings are made on the operation interface of the display device 1, the signals are transmitted to the linkage control end of the incident light optical adapter 3 and the linkage control end of the incident light optical adapter 4 respectively. The incident light path is selected by the light splitting component 9 of the linkage control end of the incident light optical adapter 3, and the corresponding incident light source signal is transmitted to the sample 10 on the microscope sample stage through the light path to realize the selection of the incident light source type (steady-state laser light source or pulse laser light source). The collected sample signal is transmitted to the fluorescence lifetime detection component or the fluorescence spectrum and microscopic imaging component according to the settings through the light splitting component 9 of the linkage control end of the incident light optical adapter 4 to realize the selection of the output light path. It is worth noting that due to the characteristics of the light source type, the CW steady-state laser light source corresponds to the FS output light path, and the pulse light source corresponds to the FLIM output light path. Only when the corresponding light source type and the output light path are adapted can the corresponding signal be collected.

[0047] Furthermore, the incident light optical adapter 3 and the incident light optical adapter 4 have the same structure, including: a motor 31, an actuator (including mutually meshing gears 32 and a long rod 33 with threads), a sliding platform 34 with a built-in reflector 36, and a magnetic barrier 35; the motor 31 receives the control information sent by the control device 2, drives the actuator to drive the sliding platform 34 to move; the magnetic barrier 35 adsorbs the sliding platform 34 when the sliding platform 34 is close; the reflector 36 is used to change the direction of the incident light path. Figure 5 The incident light optical adapter 3 is taken as an example for illustration.

[0048] See also Figure 6 As shown, the process of the control device 2 controlling the incident light optical adapter 3 to select the incident light source is as follows:

[0049] When the incident light source is a pulse light source ( Figure 6 (a)), the control device 2 sends a signal to the motor through the optical fiber data line 21, controls the sliding platform to be located at the far left, and the pulse light source enters from the first interface 37 of the incident light optical adapter 3, and is output to the sample detection component through the second interface 38 of the incident light optical adapter 3; when the selected incident light source is a steady-state light source ( Figure 6 (b)), the control device 2 sends a signal to the motor, controls the motor to drive the execution component to push the sliding platform to move to the far right until it is adsorbed by the magnetic blocker, and the steady-state light source enters from the third interface 39 of the incident light optical adapter 3, changes direction after passing through the reflector, and is output from the second interface 38 to the sample detection component.

[0050] See also Figure 7 As shown, the process of the control device 2 controlling the incident light optical adapter 4 to conduct the outgoing signal to the optical component to be introduced is as follows:

[0051] When the optical component selected to be imported is a fluorescence lifetime component ( Figure 7 (a)), the control device 2 sends a signal to the motor through the optical fiber data line 21, controls the sliding platform to be located at the far left, and the output signal output by the sample detection component enters from the second interface 48 of the incident light optical adapter 4, and is output to the fluorescence lifetime component through the first interface 47 of the incident light optical adapter 4; when the optical component to be introduced is the fluorescence microscopy imaging component ( Figure 7 (b)), the control device 2 sends a signal to the motor, controls the motor to drive the execution component to push the sliding platform to move to the far right until it is adsorbed by the magnetic blocker, and the output signal of the sample detection component enters from the second interface 48, changes direction after passing through the reflector, and is output from the third interface 49 of the incident light optical adapter 4 to the fluorescence microscopy imaging component.

[0052] In summary, the complete operation flow of this embodiment is as follows.

[0053] Set up the COM port according to the experimental requirements (usually the set COM port is COM5, Parity-None, Stop Bits-one, Baud Rate-19200, Date Bits-8). Then set up the Input Laser according to the experimental requirements. Click "Open port", and the Input Laser and Output Laser on the software interface change from the gray inactive state to the settable black state. Click "CW Laser" to select the steady-state light source. At this time, the operation interface sends the command to the control device 2 through the data cable. After receiving the command, the control device 2 transmits the command to the linkage control end of the incident light optical adapter 3 through the signal optical fiber. The linkage end hardware is configured with a motor, and the motor pulls the sliding platform of the incident light optical adapter 3 to move. This process will have the sound of the motor operation until the displacement is completed. At this time, the software interface "CW Laser" has a black frame indicating that the operation is completed. The selection of the incident light source is completed. Click "Pulse Laser" in the same way. If an emergency situation of motor displacement error occurs during this process, click "Stop" to stop the process to protect the equipment.

[0054] After completing the selection of the incident light source according to the experimental content, the direction of the outgoing light needs to be adjusted. If you need to obtain conventional fluorescence spectrum information, you need to import the FS system. The specific steps are: click "FS" in the Output Laser setting, the operation interface will send instructions to the control device 2 through the data line, and the control device 2 will receive the instructions and send signals to the motor of the linkage control end of the incident light optical adapter 4 through the optical fiber. The displacement of the sliding platform pulled by the motor completes the adjustment of the direction of the fluorescence signal collected from the sample. Click "FLIM" in the same way.

[0055] The above is only a specific implementation of the present invention, but the design concept of the present invention is not limited thereto. Any non-substantial changes to the present invention using this concept shall be deemed as an infringement of the protection scope of the present invention.

Claims

1. An incident light and outgoing light optical adapter linkage control system, characterized in that: include: Display device, control device, incident light optical adapter and outgoing light optical adapter; The control device is connected to the display device, the incident light optical adapter and the outgoing light optical adapter respectively; The display device is provided with an incident light selection module and an outgoing light output selection module; The incident light selection module is used to receive the selected incident light source; the outgoing light output selection module is used to receive the selected optical component to which the outgoing signal is to be introduced; The control device receives the signal sent by the display device, and transmits different control signals to the incident light optical adapter and the outgoing light optical adapter based on the selected incident light source and the optical component to be introduced; The incident light optical adapter receives a control signal sent by the control device, controls the selected incident light source to be incident, and transmits the corresponding incident light source to the sample; The output light optical adapter receives the control signal sent by the control device, controls the output signal of the collected sample to enter, and transmits the output signal to the optical component to be introduced.

2. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The display device is also provided with a communication port channel selection module for selecting a required communication port channel and performing optical path setting.

3. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The display device is further provided with a path selection module for controlling whether to allow the incident light selection module and the outgoing light output selection module to be selected.

4. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The incident light source selected by the incident light selection module includes a steady-state light source or a pulse light source.

5. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The incident light selection module is further used to receive a first emergency stop instruction to stop switching of the incident light source.

6. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The output light selection module receives the selected optical component to be introduced, including a fluorescence lifetime component or a fluorescence microscopy imaging component.

7. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The output light selection module is further used to receive a second emergency stop instruction to stop the switching of the optical component to be introduced.

8. The incident light and outgoing light optical adapter linkage control system according to claim 1, characterized in that: The incident light optical adapter and the outgoing light optical adapter have the same structure, including: a motor, an actuator, a sliding platform with a built-in reflector and a magnetic barrier; the motor receives control information sent by a control device, drives the actuator to drive the sliding platform to move; the magnetic barrier adsorbs the sliding platform when the sliding platform is close; the reflector is used to change the direction of the incident light path.

9. The incident light and outgoing light optical adapter linkage control system according to claim 8, characterized in that: The process of the control device controlling the incident light optical adapter to select the incident light source is as follows: When the selected incident light source is a pulse light source, the control device sends a signal to the motor to control the sliding platform to be located on the far left, and the pulse light source enters from the first interface of the incident light optical adapter and is output to the sample detection component through the second interface of the incident light optical adapter; when the selected incident light source is a steady-state light source, the control device sends a signal to the motor to control the motor to drive the execution component to push the sliding platform to the far right until it is adsorbed by the magnetic blocker, and the steady-state light source enters from the third interface of the incident light optical adapter, changes direction after passing through the reflector, and is output to the sample detection component from the second interface.

10. The incident light and outgoing light optical adapter linkage control system according to claim 8, characterized in that: The process of the control device controlling the outgoing light optical adapter to conduct the outgoing signal to the optical component to be introduced is as follows: When the optical component selected to be introduced is a fluorescence lifetime component, the control device sends a signal to the motor to control the sliding platform to be located at the far left, and the output signal output by the sample detection component enters from the second interface of the output light optical adapter, and is output to the fluorescence lifetime component through the first interface of the output light optical adapter; when the optical component selected to be introduced is a fluorescence microscopy imaging component, the control device sends a signal to the motor to control the motor to drive the execution component to push the sliding platform to the far right until it is adsorbed by the magnetic blocker, and the output signal output by the sample detection component enters from the second interface, changes direction after passing through the reflector, and is output from the third interface of the output light optical adapter to the fluorescence microscopy imaging component.

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