Production method of cordyceps sobolifera spore powder

Abstract

The invention provides a production method of cordyceps sobolifera spore powder, and belongs to the technical field of fungus cultivation. The invention provides a production method of cordyceps sobolifera spore powder, which comprises the following steps: (1) adding soy peptone and maltose on the basis of a PDA (potato dextrose agar) basic culture medium to obtain an improved PDA culture medium; (2) inoculating cordyceps sobolifera strains into the improved PDA culture medium, and culturing; and (3) after the culture is finished, collecting cordyceps sobolifera conidial powder. According to the production method of the cordyceps sobolifera conidial powder, the improved PDA culture medium serves as a culture medium, the yield of the cordyceps sobolifera conidial powder is increased, the production process is simple, operation is convenient, the period is short, economic benefits are high, and wide application value is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of fungus cultivation, in particular to a method for producing spore powder of Cordyceps sinensis. Background Art

[0002] Cordyceps sinensis has multiple functions such as improving immunity, resisting fatigue, protecting kidneys and liver, improving sleep, anti-tumor, anti-radiation and improving eyesight. It is a magical ancient Chinese medicine. Artificially cultivated Cordyceps sinensis contains 2.18% mannitol, 21.73% polysaccharides, 19.76% amino acids, 0.005% cordycepin, and 0.05% adenosine, which is similar to natural Cordyceps sinensis. Therefore, Cordyceps sinensis can be used as a substitute for Cordyceps sinensis and can also achieve the effect of nourishing and maintaining health. Cordyceps sinensis spore powder is the "seed" of Cordyceps sinensis. It not only has the function of reproduction, but also gathers the active essence of Cordyceps sinensis. It is the most active ingredient and the most effective part of Cordyceps sinensis.

[0003] In recent years, Cordyceps sinensis spore powder has been gradually used by medical workers for clinical research in the field of tumors. At the 2011 International Biomedicine and Biotechnology Forum Annual Meeting, Professor Gu Zhenlun of the School of Pharmacy of Soochow University reported his scientific research results "Preliminary Report on the Anti-tumor Effect of Cordyceps sinensis Spore Powder and Wild Cordyceps sinensis Crude Polysaccharide", pointing out that the apoptosis rate (ordered cancer cell death) of Cordyceps sinensis spore powder on pancreatic cancer, leukemia and cervical cancer cells is 8.24 times, 16.64 times and 23.44 times that of the broad-spectrum anticancer drug 5-Fu, respectively; the necrosis rate (killing) of cancer cells is 8.67 times, 287.75 times and 31.50 times that of 5-Fu, respectively. However, Cordyceps sinensis spore powder is expensive. The price of 30g Cordyceps sinensis pure powder of Pan Asia Medicine is 999 yuan. Therefore, it is of great significance to study the cultivation and application of Cordyceps sinensis spore powder.

[0004] Carbon source and nitrogen source are indispensable raw materials for edible fungi to synthesize mycelial cells and are the energy sources for their life activities. Commonly used carbon sources in culture medium are generally sugars, such as sucrose, fructose, glucose, etc., and commonly used nitrogen sources mainly include yeast powder, peptone, bran, etc. In the cultivation technology of Cordyceps militaris, CN117561922A is a method for cultivating spore powder of Cordyceps militaris, in which the carbon source used in the process of cultivating Cordyceps militaris mother species is glucose or sucrose, and the carbon source used in the process of cultivating original species is brown sugar, and the nitrogen source is peptone. CN108707527A is a method for preparing a high spore powder Cordyceps militaris wine appreciation combination and its cultivation combination, in which peptone is used as a carbon source in the formula of the Cordyceps militaris liquid culture medium, and glucose is used as a carbon source. CN111758485A is a method for cultivating Cordyceps militaris fruiting bodies rich in cordyceps acid, and a new culture medium is designed, with glucose as a carbon source and peptone as a nitrogen source. CN116925929A A Cordyceps militaris and its cultivation method and use, the carbon source is mainly glucose, and the nitrogen source is mainly peptone and yeast powder. In addition, Liu Kuanbo explored the effects of several carbon sources and nitrogen sources on the yield of cicada fungus spore powder in the article "Study on Cordyceps Resources Producing N6-(2-Hydroxyethyl) Adenosine (HEA) and HEA Biosynthesis Regulation". The carbon sources mainly used glucose, maltose, lactose and sucrose, and the nitrogen sources mainly used tryptone, A peptone, soy peptone, and yeast extract powder. The results showed that cicada fungus grew faster with sucrose and glucose as carbon sources, while maltose and sucrose grew poorly and grew relatively slowly. The dry weight test results showed that cicada fungus with glucose as carbon source grew better, and the dry weight was significantly higher than maltose and lactose. Cicada fungus with tryptone and A peptone as nitrogen sources grew better, and the dry weight was significantly higher than other cicada fungi cultivated as nitrogen sources. It can be seen that in the prior art, most of the methods for cultivating Cordyceps militaris use glucose or sucrose as a carbon source and peptone or yeast powder as a nitrogen source.

[0005] Based on this, the present invention aims to provide a method for producing spore powder of Cordyceps sinensis, so that the spore powder has a higher yield. Summary of the invention

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for producing spore powder of Cordyceps sinensis, comprising the following steps:

[0008] (1) Adding soy peptone and maltose to the PDA basal medium to obtain a modified PDA medium;

[0009] (2) inoculating Cordyceps sinensis strains into the modified PDA medium for culturing;

[0010] (3) After the culture is completed, collect the spore powder of Cordyceps sinensis.

[0011] Preferably, the added amount of soy peptone is 0.3-0.6% of the mass of the PDA basic culture medium, and the added amount of maltose is 0.5-1.0% of the mass of the PDA basic culture medium.

[0012] Preferably, the preparation method of the PDA basic culture medium is as follows: potato pieces are mixed with water in a ratio of 180-220 g:1000 mL and boiled for 20-25 min, filtered through gauze, the filtrate is added with water to make up to 1000 mL, and then glucose and agar are added to obtain the PDA basic culture medium.

[0013] Preferably, the mass ratio of glucose to agar to potato is 18-22:18-22:180-220.

[0014] Preferably, the inoculation amount of the Cordyceps sinensis fungus is 5-7%.

[0015] Preferably, the culture conditions are: culture at 20-25° C. for 30-40 days.

[0016] The production method of Cordyceps sinensis spore powder provided by the invention uses the improved PDA culture medium as the cultivation matrix, thereby improving the yield of Cordyceps sinensis spore powder, and has the advantages of simple production process, convenient operation, short cycle, high economic benefit and broad application value. DETAILED DESCRIPTION

[0017] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0018] Example 1

[0019] PDA basal culture medium: 200g potatoes, wash, peel and cut into small pieces, add 1000mL of water, boil for 25min, filter with gauze, take the filtrate and add water to make up to 1000mL, then add 20g glucose and 20g agar, and heat to fully dissolve the glucose and agar.

[0020] Nitrogen source and carbon source were added to every 100 mL of PDA basal medium according to Table 1 to obtain a modified PDA medium.

[0021] The Cordyceps sinensis strain was collected from cicada fungi in the bamboo forest of Shoushan Village, Fuzhou, and obtained through tissue separation and purification.

[0022] The modified PDA medium was placed in a 400 mL culture bottle at 25 ml per bottle, sterilized at 121°C for 25 minutes, and inoculated after cooling. The Cordyceps sinensis spawn was cut into 0.3*0.3 seed blocks (5%), and one block of spawn was inoculated into each bottle. Then, the spawn was cultured at 25°C for 40 days. After the culture was completed, the Cordyceps sinensis spore powder was collected and the yield was calculated. The results are shown in Table 1.

[0023] Table 1 Results of spore production by adding different carbon and nitrogen sources to PDA culture

[0024]

[0025] It can be seen from Table 1 that using the maltose of the present invention as a carbon source and soy peptone as a nitrogen source can greatly increase the yield of Cordyceps sinensis spore powder, which is different from the conclusion taught in the prior art that using glucose or sucrose as a carbon source and peptone or yeast powder as a nitrogen source is beneficial to the growth of Cordyceps sinensis.

[0026] Example 2

[0027] A method for producing spore powder of Cordyceps sinensis comprises the following steps:

[0028] PDA basal culture medium: 180g potatoes, wash, peel and cut into small pieces, add 1000mL of water, boil for 25min, filter with gauze, take the filtrate and add water to make up to 1000mL, then add 18g glucose and 18g agar, and heat to fully dissolve the glucose and agar.

[0029] The modified PDA medium was obtained by adding 0.5 g of soy peptone and 1.0 g of maltose per 100 mL of PDA basal medium.

[0030] The Cordyceps sinensis strain was collected from cicada fungi in the bamboo forest of Shoushan Village, Fuzhou, and obtained through tissue separation and purification.

[0031] The modified PDA medium was placed in a 400 mL culture bottle at 25 ml per bottle, sterilized at 121°C for 25 minutes, and inoculated after cooling. The Cordyceps sinensis fungus was cut into 0.3*0.3 pieces, and one piece of fungus was inoculated into each bottle. Then, the culture was placed at 25°C for 40 days. After the culture was completed, the Cordyceps sinensis spore powder was collected, and the yield per bottle was between 0.8 and 1.0 g.

[0032] Example 3

[0033] A method for producing spore powder of Cordyceps sinensis comprises the following steps:

[0034] PDA basal culture medium: Wash and peel 220g potatoes, cut into small pieces, add 1000mL of water, boil for 25min, filter through gauze, take the filtrate and add water to make up to 1000mL, then add 22g of glucose and 22g of agar, and heat to fully dissolve the glucose and agar.

[0035] The modified PDA medium was obtained by adding 0.5 g of soy peptone and 1.0 g of maltose per 100 mL of PDA basal medium.

[0036] The Cordyceps sinensis strain was collected from cicada fungi in the bamboo forest of Shoushan Village, Fuzhou, and obtained through tissue separation and purification.

[0037] The modified PDA medium was placed into 400 mL culture bottles at 25 ml per bottle, sterilized at 121°C for 25 minutes, and inoculated after cooling. The Cordyceps sinensis spawn was cut into 0.3*0.3 pieces, and one piece of spawn was inoculated into each bottle. Then it was cultured at 25°C for 40 days. After the culture was completed, the Cordyceps sinensis spore powder was collected, and the average yield per bottle was 0.9 g.

[0038] Example 4

[0039] A Cordyceps sinensis spore powder lozenge was prepared using the Cordyceps sinensis spore powder produced in Example 1.

[0040] Formula 1: 90 parts by weight of Cordyceps sinensis spore powder, 10 parts by weight of povidone, 1.0 parts by weight of glycerol and 10 parts of water.

[0041] Formula 2: 95 parts by weight of Cordyceps sinensis spore powder, 5 parts by weight of povidone, 1.0 parts by weight of glycerol and 10 parts of water.

[0042] Formula 3: 90 parts by weight of Cordyceps sinensis spore powder, 5 parts by weight of povidone, 0.5 parts by weight of glycerol and 8 parts of water.

[0043] Formula 4: 95 parts by weight of Cordyceps sinensis spore powder, 10 parts by weight of povidone, 1.5 parts by weight of glycerol and 10 parts of water.

[0044] The above formula is mixed evenly according to the proportion, bottled, sterilized (115°C, 20min), cooled and made into Cordyceps sinensis spore powder tablets by tablet making machine. Povidone is a water-soluble high molecular polymer with good adhesion and compressibility, and the tableting effect is better than starch.

[0045] As can be seen from the above embodiments, the present invention provides a method for producing Cordyceps sinensis spore powder. On the basis of PDA basic medium, maltose is added as a carbon source and soy peptone is added as a nitrogen source, and the yield of Cordyceps sinensis spore powder is greatly improved compared with using brown sugar, white sugar or glucose as a carbon source and yeast powder, peptone or yeast extract as a nitrogen source. Finally, it is prepared into Cordyceps sinensis spore powder lozenges, and the Cordyceps sinensis spore powder has high purity and is easy to eat.

[0046] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for producing spore powder of Cordyceps sinensis, characterized in that: The steps include: (1) Adding soy peptone and maltose to the PDA basal medium to obtain a modified PDA medium; (2) inoculating Cordyceps sinensis strains into the modified PDA medium for culturing; (3) After the culture is completed, collect the spore powder of Cordyceps sinensis.

2. The production method according to claim 1, characterized in that 0.3-0.6 g of the soy peptone and 0.5-1.0 g of maltose are added to every 100 mL of the PDA basic culture medium.

3. The production method according to claim 2, characterized in that The preparation method of the PDA basic culture medium is as follows: potato pieces are mixed with water in a ratio of 180-220 g:1000 mL, boiled for 20-25 min, filtered through gauze, the filtrate is supplemented with water to 1000 mL, and then glucose and agar are added to obtain the PDA basic culture medium.

4. The production method according to claim 3, characterized in that The mass ratio of the glucose, agar and potato is 18-22:18-22:180-220.

5. The production method according to claim 4, characterized in that The inoculation amount of the Cordyceps sinensis fungus is 5-7%.

6. The production method according to claim 5, characterized in that The culture conditions are: culture at 20-25° C. for 30-40 days.

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