Mushroom strain rotating tube cultivation equipment

Abstract

The invention belongs to the technical field of strain rotating tubes, and discloses mushroom strain rotating tube cultivation equipment which comprises a semitransparent sterile box, a sealing box door is hinged to one end of the semitransparent sterile box, a telescopic universal arm sleeve is fixedly connected to the sealing box door in a penetrating mode, an alcohol lamp is connected into the inner wall of the bottom end of the semitransparent sterile box in an attached mode, and the inner wall of the bottom end of the semitransparent sterile box is fixedly connected with the telescopic universal arm sleeve in a penetrating mode. The horizontally-moving threaded sleeve can drive the L-shaped groove plate and the inoculation brush rod to synchronously move and stretch into a test tube needing to be rotated, the contact area of the inoculation brush rod is increased to pick up strains, the annular sleeve abutting plate can abut against the supporting rod under translation to drive the inoculation brush rod to contract towards the interior of the L-shaped groove plate, and then the strains on the inoculation brush rod can be blocked by the L-shaped groove plate; when the inoculation brush rod is contracted, the strains are gradually separated from the contracted inoculation brush rod, the taken strains naturally fall down and are attached to the interior of the agar culture medium blank test tube, and the tube transfer is successful, so that manual operation is reduced, and meanwhile, damage to the strains and reduction of activity and purity during tube transfer of the strains can be avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of mushroom strain transfer tubes, and in particular relates to mushroom strain transfer tube cultivation equipment. Background Art

[0002] Mushroom strains are propagation materials composed of mycelium and growth matrix of edible fungi, industrial fungi, etc. They are basic materials for microbiology and life science research. They are widely used in medicine, industry, agriculture and other fields. Their screening, preservation and utilization are of great significance to promoting the development of related industries. In the production of edible fungi strains, test tube culture strains are generally called mother strains or slant strains. The expansion culture of slant strains is called subculture, commonly known as transfer tubes. In a sterile room or inoculation box, hold the test tube to be transferred and the blank test tube of agar medium in the left hand, hold the inoculation spatula in the right hand, place the test tube mouth in the sterile area formed by the flame of the alcohol lamp, pick up a small piece of strains with the inoculation spatula, and connect it to the center of the blank culture tube. After inoculation, burn the tube mouth on the flame of the alcohol lamp, plug it with cotton, and then connect another blank test tube. Through multiple transfers, that is, strain activation, the strains can gradually adapt to the culture environment, obtain pure and strong cultures, and improve yield and quality.

[0003] At present, the CN201248286Y patent discloses a machine for transferring edible fungi mother culture to tubes. The utility model relates to an edible fungi production machine, and more particularly to a machine for transferring edible fungi mother culture to tubes. The innovation of this machine for transferring tubes lies in the design and installation of a mother culture container, an intermittent propulsion mechanism for the mother culture container piston rod, and a reduction motor equipped with a driving eccentric wheel; the driving eccentric wheel rotates to the working position, the propulsion connecting rod is pressed down, and the propulsion cap pushes the mother culture container piston rod forward, and the fungi are quantitatively discharged from the front end discharge port of the mother culture container and fall on the blank agar culture medium in the test tube. This machine for transferring edible fungi mother culture to tubes achieves the purpose of simple manufacturing process of the whole machine, convenient disassembly and assembly, low production cost, stable and continuous transfer of mother culture, uniform dosage of transfer, fast transfer speed, and significantly improved production efficiency compared with manual transfer.

[0004] However, the piston quantitative discharge method involved in the above-mentioned device may increase the risk of strain degradation if the strain pushed out by the piston is improperly operated or the tube is turned too many times during the tube transfer process. In addition, mechanical damage and environmental pollution during the tube transfer process may also have adverse effects on the strain, which is likely to reduce the vitality and purity of the strain. Summary of the invention

[0005] In order to solve the problems raised in the above background technology, the present invention provides a mushroom spawn transfer tube cultivation device.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a mushroom spawn transfer tube cultivation device, comprising a translucent sterile box, a sealed box door is hinged on one end of the translucent sterile box, a telescopic universal arm sleeve is also fixedly connected to the sealed box door, an alcohol lamp is fittedly connected to the inner wall of the bottom end of the translucent sterile box, a magnetic plate is fixedly connected to the inner wall of one end of the translucent sterile box near the top, a plurality of sealing plugs are arranged below the magnetic plate, each of the sealing plugs is respectively composed of two parts, the upper half is made of metal material, and the lower half is made of rubber material, an L-shaped cardboard is fixedly connected to the side wall of the bottom end of the translucent sterile box, and two test tubes can be intermittently and tightly clamped on the top of the L-shaped cardboard, and the two test tubes are respectively a tube to be transferred and a blank tube of agar culture medium;

[0007] A rotary tube cultivation section is provided in the translucent sterile box, and the rotary tube cultivation section includes a motor of a forward and reverse rotation model. A screw is fixedly connected to the rotating shaft of the motor, and a slider is fixedly connected to a section of the screw body. A rectangular groove for sliding with the slider is provided on the end corresponding to the slider, and a folding sealing plate is fixedly connected between the inner walls at both ends of the rectangular groove and the outer walls at both ends of the slider. A threaded sleeve is threadedly connected to the screw body, and an L-shaped groove plate is fixedly connected to the outer wall of one end of the threaded sleeve, and an inoculation brush rod is fitted in the side wall groove of the L-shaped groove plate. The inoculation brush rod can be indirectly driven by the motor to transfer the bacteria in one test tube to another test tube for rotary tube cultivation.

[0008] Preferably, a collar T-shaped hinge plate is fixedly connected to an outer wall of one side of the threaded sleeve, and a T-shaped groove for sliding engagement with one end of the collar T-shaped hinge plate is provided in the bottom side wall of the translucent sterile box.

[0009] Preferably, two springs are fixedly connected to the smooth outer wall at one end of the inoculation brush rod, and the other ends of the two springs are fixedly connected to the inner wall of one end of the L-shaped groove plate. A support rod is fixedly connected to the outer wall of the side end of the inoculation brush rod, and a circular arc groove is formed on one end of the L-shaped groove plate, and the rod body of the support rod and the inner wall of the circular arc groove are fitted and slidably connected.

[0010] Preferably, a U-shaped abutment plate is fixedly connected to the plate body of the L-shaped groove plate, and the other end of the support rod is fixedly connected to the outer wall of one end of the U-shaped abutment plate, and a sliding disc hinge plate is fit-fitted on the outer wall of the threaded sleeve, and the outer wall of one end of the sliding disc hinge plate and the outer wall of the other end of the U-shaped abutment plate are intermittently fit-connected.

[0011] Preferably, two springs 2 are fixedly connected to the outer wall of the other end of the sliding disc hinge plate, and one side plate of the sliding disc hinge plate is specifically connected to one end of the translucent sterile box in a penetrating, fitting and sliding manner.

[0012] Preferably, an elastic telescopic member is slidably connected to one end of the sliding plate hinge plate away from the plate body, and an inclined support plate is fixedly connected to the other end of the elastic telescopic member. The inclined support plate is fixedly connected to the inner wall of the translucent sterile box.

[0013] Preferably, a limiting slide plate is provided on one end of the inclined support plate, the other ends of the two springs are fixedly connected to the outer wall of one end of the limiting slide plate, and sliding grooves are provided in the inner walls at both ends of the inclined support plate.

[0014] Preferably, the upper and lower side plates of the limiting slide are respectively slidably connected to the inner walls of the two slide grooves, two locking balls are fixedly connected to the side wall at one end of the limiting slide, and two locking grooves that can be tightly engaged with the two locking balls are provided in the side wall at one end of the inclined support plate.

[0015] Preferably, an L-shaped tooth plate is fixedly connected to the rod body of the support rod, a spur gear is provided above the L-shaped tooth plate, the spur gear and the L-shaped tooth plate can be intermittently meshed, and a telescopic rod is fixedly connected through the spur gear.

[0016] Preferably, the rod body on one side of the telescopic rod and the inner wall of one end of the translucent sterile box are movably connected, and the rod body on the other side of the telescopic rod is provided with a thread, and the rod body with the thread is also threadedly connected with a ring sleeve abutment plate, and the ring sleeve abutment plate is specifically divided into two parts, one part is a ring sleeve disk that rotates inwardly but not outwardly, and the other part is a natural rubber plate with high elasticity.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention can drive the screw and the threaded sleeve to rotate by starting the motor, and the passive threaded sleeve will be limited by the fitting of the connected collar T-shaped hinge plate in the T-shaped groove, so as to help the threaded sleeve to perform horizontal guiding translation on the screw, and thus the translated threaded sleeve will move synchronously with the L-shaped groove plate and the inoculation brush rod, and probe into the test tube that needs to be rotated, so that the inoculation brush rod increases the contact area to pick up the bacterial strain;

[0019] The present invention can also drive the L-shaped toothed plate to perform synchronous translation through the passive movement of the L-shaped groove plate again. When it moves to a certain spacing, the top end of the L-shaped toothed plate will mesh with the spur gear, thereby meshing and driving the spur gear and the telescopic rod to rotate synchronously. The rotating telescopic rod will drive the ring sleeve abutment plate to rotate inwardly but not outwardly through the opened thread. As a result, the ring sleeve abutment plate will contact the support rod under translation, driving the inoculation brush rod to shrink into the L-shaped groove plate, and then the bacteria on the inoculation brush rod will be blocked by the L-shaped groove plate, thereby gradually separating from the contracted inoculation brush rod, and the taken out bacteria will naturally fall and adhere to the blank test tube of the agar culture medium, and the tube transfer will be successful. The above can reduce manual operation and avoid harm to the bacteria during tube transfer, thereby reducing their vitality and purity. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 It is a schematic diagram of the overall bottom-up structure of the present invention;

[0022] Figure 3 This is a schematic diagram of the overall structure of the rotary tube cultivation part of the present invention;

[0023] Figure 4 This is a schematic diagram of the partial disassembly structure of the rotary tube cultivation part of the present invention (part 1);

[0024] Figure 5 For the present invention Figure 4 A schematic diagram of the partially enlarged structure at center A;

[0025] Figure 6 This is a schematic diagram of the partial disassembly structure of the rotary tube cultivation part of the present invention (part 2);

[0026] Figure 7 It is a schematic diagram of the local structure of the rotary tube cultivation part of the present invention;

[0027] Figure 8 It is a schematic diagram of the overall partial cross-sectional structure of the present invention.

[0028] In the figure:

[0029] 1. Translucent sterile box; 11. Sealed box door; 12. Telescopic universal arm sleeve; 13. Alcohol lamp; 14. Magnetic plate; 15. Sealing plug; 16. L-shaped cardboard; 17. Test tube;

[0030] 2. Rotating tube cultivation part; 21. Motor; 22. Screw; 23. Threaded sleeve; 2301. T-shaped hinge plate with collar; 2302. T-shaped slot; 24. L-shaped slot plate; 25. Inoculation brush rod; 26. Spring 1; 27. Support rod; 28. Arc slot; 29. ​​U-shaped abutment plate; 230. Sliding disc hinge plate; 231. Spring 2; 232. Elastic telescopic part; 233. Oblique support plate; 234. Limiting slide plate; 235. Slide groove; 236. Card ball; 237. Card slot; 238. L-shaped tooth plate; 239. Spur gear; 240. Telescopic rod; 241. Ring sleeve abutment plate. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] like Figures 1 to 8 As shown, the present invention provides a mushroom spawn tube transfer cultivation device, comprising a translucent sterile box 1, a sealed box door 11 is hinged on one end of the translucent sterile box 1, a telescopic universal arm sleeve 12 is also fixedly connected to the sealed box door 11, an alcohol lamp 13 is fitted and connected to the inner wall of the bottom end of the translucent sterile box 1, a magnetic plate 14 is fixedly connected to the inner wall of one end of the translucent sterile box 1 close to the top, a plurality of sealing plugs 15 are arranged below the magnetic plate 14, each sealing plug 15 is respectively composed of two parts, the upper half is made of metal material, and the lower half is made of rubber material, an L-shaped card plate 16 is fixedly connected to the side wall of the bottom end of the translucent sterile box 1, and two test tubes 17 can be intermittently and tightly clamped on the top of the L-shaped card plate 16, and the two test tubes 17 are respectively a tube to be transferred and a blank tube of agar culture medium;

[0033] A rotary tube cultivation section 2 is provided in the translucent sterile box 1, and the rotary tube cultivation section 2 includes a forward and reverse rotating motor 21, a screw rod 22 is fixedly connected to the rotating shaft of the motor 21, a slider is fixedly connected to a section of the rod body of the screw rod 22, and a rectangular groove for sliding with the slider is provided on the end corresponding to the slider, and a folding sealing plate is fixedly connected between the inner walls at both ends of the rectangular groove and the outer walls at both ends of the slider, a threaded sleeve 23 is threadedly connected to the rod body of the screw rod 22, an L-shaped groove plate 24 is fixedly connected to the outer wall of one end of the threaded sleeve 23, and an inoculation brush rod 25 is fitted in the side wall groove of the L-shaped groove plate 24. The inoculation brush rod 25 can be indirectly driven by the motor 21 to transfer the bacteria in one test tube 17 to another test tube 17 for rotary tube cultivation.

[0034] The above scheme is adopted: by opening the sealed box door 11 on one end of the translucent sterile box 1, two test tubes 17, which are the tube to be transferred and the blank tube of agar culture medium, can be put in and taken out of the translucent sterile box 1 respectively. The telescopic universal arm sleeve 12 is manually extended into the translucent sterile box 1 with one hand. The telescopic universal arm sleeve 12 can produce a sealing effect on the connection between the arm and the translucent sterile box 1 while not limiting the movement of the arm, so that the two test tubes 17 can be respectively clamped in the top end of the L-shaped clamping plate 16, and the starting motor 21 can drive the screw 22 and the threaded sleeve 23 to rotate.

[0035] A collar T-shaped hinge plate 2301 is fixedly connected to the outer wall of one side of the threaded sleeve 23, and a T-shaped groove 2302 is provided in the bottom side wall of the translucent sterile box 1 for sliding engagement with one end of the collar T-shaped hinge plate 2301. Two springs 26 are fixedly connected to the smooth outer wall of one end of the inoculation brush rod 25, and the other ends of the two springs 26 are fixedly connected to the inner wall of one end of the L-shaped groove plate 24. A support rod 27 is fixedly connected to the outer wall of the side end of the inoculation brush rod 25. A circular arc groove 28 is formed on one end of the groove plate 24, and the rod body of the support rod 27 is fitted and slidably connected to the inner wall of the circular arc groove 28. A U-shaped support plate 29 is fixedly connected to the plate body of the L-shaped groove plate 24, and the other end of the support rod 27 is fixedly connected to the outer wall of one end of the U-shaped support plate 29. A sliding disc hinge plate 230 is fitted and sleeved on the outer wall of the threaded sleeve 23, and the outer wall of one end of the sliding disc hinge plate 230 is intermittently fitted and connected to the outer wall of the other end of the U-shaped support plate 29.

[0036] The above scheme is adopted: the passive threaded sleeve 23 will pass through the connected ring T-shaped hinge plate 2301 to fit and limit in the T-shaped groove 2302, thereby helping the threaded sleeve 23 to perform horizontal guided translation on the screw 22, and the translated threaded sleeve 23 will move synchronously with the L-shaped groove plate 24 and the inoculation brush rod 25, and probe into the test tube 17 that needs to be rotated, so that the inoculation brush rod 25 increases the contact area to pick up the bacterial strain, and then the screw 22 reverses, so that it indirectly drives the L-shaped groove plate 24 to reset, and after resetting, it will passively move a certain distance again, thereby causing the U-shaped abutment plate 29 to press against the sliding disc hinge plate 230.

[0037] Two springs 231 are fixedly connected to the outer wall of the other end of the sliding disc hinge plate 230. One side of the sliding disc hinge plate 230 is specifically connected to one end of the translucent sterile box 1 in a sliding manner. An elastic telescopic member 232 is slidably connected to the end of the sliding disc hinge plate 230 away from the plate body. An oblique support plate 233 is fixedly connected to the other end of the elastic telescopic member 232. The oblique support plate 233 is fixedly connected to the inner wall of the translucent sterile box 1. A limiting slide plate 234 is provided on one end of the oblique support plate 233. The other ends of the two springs 231 are fixedly connected to the outer wall of one end of the limiting slide plate 234, and sliding grooves 235 are provided in the inner walls of both ends of the inclined support plate 233. The upper and lower side plates of the limiting slide plate 234 are respectively fitted and slidably connected with the inner walls of the two sliding grooves 235. Two locking balls 236 are fixedly connected to the side wall of one end of the limiting slide plate 234, and two locking grooves 237 that can be tightly engaged with the two locking balls 236 are provided in the side wall of one end of the inclined support plate 233.

[0038] The above scheme is adopted: the U-shaped support plate 29 presses against the sliding disc hinge plate 230, driving it to slide on the threaded sleeve 23 and squeeze the two springs 231. Once the sliding disc hinge plate 230, which is subjected to force and translates, stops pressing the elastic telescopic part 232, the elastic telescopic part 232, which is no longer subjected to force, will immediately eject and fit into contact with the outer wall of the L-shaped slot plate 24, thereby driving the L-shaped slot plate 24 and other related structures to translate horizontally through the limiting slide plate 234 in the inclined support plate 233 and under the limitation of the two slide grooves 235. The two cards 236 will be immediately disengaged from the card connection of the card groove 237 by the sudden impact push of the elastic telescopic part 232, and will not affect the sliding of the limiting slide plate 234.

[0039] An L-shaped toothed plate 238 is fixedly connected to the rod body of the support rod 27, and a spur gear 239 is provided above the L-shaped toothed plate 238. The spur gear 239 and the L-shaped toothed plate 238 can be intermittently meshed with each other. A telescopic rod 240 is fixedly connected through the spur gear 239. One side of the telescopic rod 240 is movably connected to the inner wall of one end of the translucent sterile box 1. A thread is provided on the other side of the telescopic rod 240, and a ring sleeve abutment plate 241 is also threadedly connected to the threaded rod body. The ring sleeve abutment plate 241 is specifically divided into two parts, one part is a ring sleeve disk that rotates inside and does not rotate outside, and the other part is a natural rubber plate with high elasticity.

[0040] The above scheme is adopted: after the L-shaped groove plate 24 is passively moved again, the L-shaped tooth plate 238 will be driven to translate synchronously. When it moves to a certain distance, the top of the L-shaped tooth plate 238 will mesh with the spur gear 239, thereby the meshing drives the spur gear 239 and the telescopic rod 240 to rotate synchronously, and the rotating telescopic rod 240 will drive the ring sleeve abutment plate 241 to rotate inwardly without rotating outwardly through the opened thread, so that the ring sleeve abutment plate 241 will contact the support rod 27 under translation, driving the inoculation brush rod 25 to shrink into the L-shaped groove plate 24, and then the bacteria on the inoculation brush rod 25 will be blocked by the L-shaped groove plate 24, so as to gradually break away from the shrinking inoculation brush rod 25, and the taken out bacteria will naturally fall and adhere to the agar medium blank test tube 17, and the tube transfer is successful.

[0041] The working principle and use process of the present invention are as follows: by opening the sealed box door 11 on one end of the translucent sterile box 1, two test tubes 17, which are the tube to be transferred and the blank tube of agar medium, can be put in and taken out of the translucent sterile box 1. The telescopic universal arm sleeve 12 is used to manually extend into the translucent sterile box 1 with one hand. The telescopic universal arm sleeve 12 can produce a sealing effect on the connection between the arm and the translucent sterile box 1 without limiting the movement of the arm, so that the two test tubes 17 can be respectively connected to the corresponding The top of the L-shaped card plate 16 is then started, and the motor 21 is started to drive the screw 22 and the threaded sleeve 23 to rotate. The passive threaded sleeve 23 will pass through the connected collar T-shaped hinge plate 2301 and fit in the T-shaped slot 2302 to help the threaded sleeve 23 to perform horizontal guided translation on the screw 22. The translated threaded sleeve 23 will move synchronously with the L-shaped slot plate 24 and the inoculation brush rod 25, and probe into the test tube 17 that needs to be transferred, so that the inoculation brush rod 25 increases the contact area to pick up the bacteria. The screw rod 22 then reverses, indirectly driving the L-shaped slot plate 24 to reset, and after resetting, it will passively move a certain distance, thereby causing the U-shaped support plate 29 to press against the sliding disc hinge plate 230, driving it to slide on the threaded sleeve 23 and squeeze the two springs 231. Once the sliding disc hinge plate 230, which has been subjected to force and has translated, stops pressing the elastic telescopic member 232, the elastic telescopic member 232, which is no longer subjected to force, will immediately eject and fit into contact with the outer wall of the L-shaped slot plate 24, thereby driving the L-shaped slot plate 24 and other related The limit slide 234 is horizontally guided and translated in the inclined support plate 233 and under the limitation of the two slide grooves 235. The elastic telescopic member 232 is suddenly pushed with impact force, which will immediately disengage the two card balls 236 from the card slots 237, and will not affect the sliding of the limit slide 234. The subsequent motor 21 will rotate forward again, and also drive the L-shaped slot plate 24, the inoculation brush rod 25 and the bacteria on the inoculation brush rod 25 into another agar medium blank test tube 17.

[0042] After the L-shaped groove plate 24 is passively moved again, it will also drive the L-shaped toothed plate 238 to translate synchronously until the inoculation brush rod 25 and the bacteria species move to a certain distance in the agar medium blank test tube 17, and the top of the L-shaped toothed plate 238 will mesh with the spur gear 239, thereby meshing and driving the spur gear 239 and the telescopic rod 240 to rotate synchronously, and the rotating telescopic rod 240 will drive the ring sleeve abutment plate 241 to rotate inwardly without rotating outwardly through the opened thread, so that the ring sleeve abutment plate 241 will contact the support rod 27 under translation, so that the support rod 27 translates in the circular arc groove 28, and at the same time drives the inoculation brush rod 25 to contract and squeeze the two springs 26 into the L-shaped groove plate 24, and the two springs 26 are convenient for driving the inoculation brush rod 25 to reset when it is no longer pressed in the future, so that the inoculation brush rod 2 The bacteria on 5 will be blocked by the L-shaped groove plate 24, so as to gradually break away from the contracted inoculation brush rod 25, and the taken out bacteria will naturally fall and adhere to the blank test tube 17 of the agar medium, and the tube transfer is successful. After the tube transfer is successful, it is necessary to manually separate the test tube 17 with the agar medium from the L-shaped clamping plate 16 with one hand, and then burn its tube mouth with the burning alcohol lamp 13, and then tilt it so that the tube mouth is upward, and then drive the test tube 17 to move upward, so that the tube mouth and the sealing plug 15 are clamped and sealed, and the sliding disc hinge plate 230 is pushed to drive the limiting slide plate 234 to reset. The above can reduce manual operation while avoiding harm to the bacteria when transferring the tube, so as to reduce its vitality and purity. According to the tube transfer operation of the prior art, the tube transfer efficiency can be effectively improved when the tube transfer is standardized.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mushroom spawn transfer tube cultivation device, comprising a translucent sterile box (1), characterized in that: A sealed box door (11) is hinged on one end of the translucent sterile box (1), and a telescopic universal arm sleeve (12) is also fixedly connected to the sealed box door (11). An alcohol lamp (13) is fitted and connected to the inner wall of the bottom end of the translucent sterile box (1). A magnetic plate (14) is fixedly connected to the inner wall of one end of the translucent sterile box (1) near the top. A plurality of sealing plugs (15) are provided below the magnetic plate (14), and each of the sealing plugs (15) is composed of two parts, the upper half of which is made of metal and the lower half of which is made of rubber. An L-shaped cardboard (16) is fixedly connected to the side wall of the bottom end of the translucent sterile box (1), and two test tubes (17) can be intermittently and tightly clamped on the top of the L-shaped cardboard (16), and the two test tubes (17) are respectively a tube to be transferred and a blank tube of agar culture medium; The translucent sterile box (1) is provided with a rotary tube cultivation section (2), and the rotary tube cultivation section (2) includes a forward and reverse rotating motor (21). A screw rod (22) is fixedly connected to the rotating shaft of the motor (21), and a slider is fixedly connected to a section of the rod body of the screw rod (22). A rectangular groove for sliding with the slider is provided at one end corresponding to the slider, and a folding sealing plate is fixedly connected between the inner walls at both ends of the rectangular groove and the outer walls at both ends of the slider. A threaded sleeve (23) is threadedly connected to the rod body of the screw rod (22), and an L-shaped groove plate (24) is fixedly connected to the outer wall of one end of the threaded sleeve (23). An inoculation brush rod (25) is fitted in the side wall groove of the L-shaped groove plate (24). The inoculation brush rod (25) can be indirectly driven by the motor (21) to transfer the bacterial species in one test tube (17) to another test tube (17) for rotary tube cultivation.

2. The mushroom spawn transfer tube cultivation equipment according to claim 1, characterized in that: A collar T-shaped hinge plate (2301) is fixedly connected to one side outer wall of the threaded sleeve (23), and a T-shaped groove (2302) for sliding engagement with one end of the collar T-shaped hinge plate (2301) is provided in the bottom side wall of the translucent sterile box (1).

3. The mushroom spawn transfer tube cultivation equipment according to claim 2, characterized in that: Two springs (26) are fixedly connected to the smooth outer wall at one end of the inoculation brush rod (25), and the other ends of the two springs (26) are fixedly connected to the inner wall at one end of the L-shaped groove plate (24). A support rod (27) is fixedly connected to the outer wall of the side end of the inoculation brush rod (25), and a circular arc groove (28) is formed through one end of the L-shaped groove plate (24), and the rod body of the support rod (27) and the inner wall of the circular arc groove (28) are fitted and slidably connected.

4. The mushroom spawn transfer tube cultivation equipment according to claim 3, characterized in that: A U-shaped abutment plate (29) is fixedly connected to the plate body of the L-shaped groove plate (24), and the other end of the support rod (27) is fixedly connected to the outer wall of one end of the U-shaped abutment plate (29). A sliding disc hinge plate (230) is fitted and sleeved on the outer wall of the threaded sleeve (23), and the outer wall of one end of the sliding disc hinge plate (230) is intermittently fitted and connected to the outer wall of the other end of the U-shaped abutment plate (29).

5. The mushroom spawn transfer tube cultivation equipment according to claim 4, characterized in that: Two springs 2 (231) are fixedly connected to the outer wall of the other end of the sliding disc hinge plate (230), and a side plate body of the sliding disc hinge plate (230) is specifically connected to one end of the translucent sterile box (1) in a penetrating, fitting and sliding manner.

6. The mushroom spawn transfer tube cultivation equipment according to claim 5, characterized in that: An elastic telescopic member (232) is slidably connected to one end of the sliding disc hinge plate (230) away from the plate body, and an inclined support plate (233) is fixedly connected to the other end of the elastic telescopic member (232). The inclined support plate (233) is fixedly connected to the inner wall of the translucent sterile box (1).

7. The mushroom spawn transfer tube cultivation equipment according to claim 6, characterized in that: A limiting slide plate (234) is provided on one end of the inclined support plate (233), and the other ends of the two springs (231) are fixedly connected to the outer wall of one end of the limiting slide plate (234). Slide grooves (235) are provided in the inner walls of both ends of the inclined support plate (233).

8. The mushroom spawn transfer tube cultivation equipment according to claim 7, characterized in that: The upper and lower side plates of the limiting slide plate (234) are respectively fitted and slidably connected with the inner walls of the two sliding grooves (235), and two locking balls (236) are fixedly connected to the side wall at one end of the limiting slide plate (234), and two locking grooves (237) that can be tightly locked with the two locking balls (236) are provided in the side wall at one end of the inclined support plate (233).

9. The mushroom spawn transfer tube cultivation equipment according to claim 8, characterized in that: An L-shaped toothed plate (238) is fixedly connected to the rod body of the support rod (27), a spur gear (239) is provided above the L-shaped toothed plate (238), the spur gear (239) and the L-shaped toothed plate (238) can be intermittently meshed, and a telescopic rod (240) is fixedly connected to the spur gear (239).

10. The mushroom spawn transfer tube cultivation equipment according to claim 9, characterized in that: The rod body on one side of the telescopic rod (240) is movably connected to the inner wall of one end of the translucent sterile box (1), and the rod body on the other side of the telescopic rod (240) is provided with a thread, and the rod body provided with the thread is also threadedly connected to a ring sleeve abutment plate (241), and the ring sleeve abutment plate (241) is specifically divided into two parts, one part is a ring sleeve disk that rotates inwardly but does not rotate outwardly, and the other part is a natural rubber plate with high elasticity.

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