Natural plant extract feed additive for relieving weaning stress of lambs and application of natural plant extract feed additive

Abstract

The invention discloses a natural plant extract feed additive for relieving weaning stress of lambs and application, and relates to the technical field of lamb breeding. The feed additive for relieving the weaning stress of the lambs is isochlorogenic acid, and the addition amount of the isochlorogenic acid in the feed is 1.5 mg / g. By adding a specific amount of isochlorogenic acid into the feed for the weaned lambs, the weaning stress reaction of the lambs can be remarkably relieved, the body weight can be increased, the feed-gain ratio can be reduced, and the feed has important popularization value in the lamb breeding industry.

Description

Technical Field

[0001] The invention relates to the technical field of lamb breeding, and more particularly to a natural plant extract feed additive for alleviating lamb weaning stress and an application thereof. Background Art

[0002] Weaning lambs often causes indigestion, diarrhea, growth stagnation and other phenomena due to the weaning of breast milk. If lambs are weaned too early, the lambs will have obvious stress reactions, which are manifested as reduced disease resistance and increased morbidity. If weaning is too late, the ewe will have poor fatness, reduced utilization, and increased breeding costs.

[0003] Therefore, providing a product or method for alleviating lamb weaning stress is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0004] In view of this, the present invention provides a natural plant extract feed additive for alleviating lamb weaning stress and its application.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A natural plant extract feed additive for alleviating lamb weaning stress. The additive is isochlorogenic acid, and the amount of the additive in the feed is 1.5 mg / g.

[0007] Another object of the present invention is to provide the use of isochlorogenic acid in the preparation of feed for alleviating lamb weaning stress, wherein the amount of isochlorogenic acid added to the basic feed is 1.5 mg / g.

[0008] Another object of the present invention is to provide a feed for alleviating weaning stress in lambs, wherein the feed comprises 1.5 mg / g of isochlorogenic acid.

[0009] Preferably, the feed also includes a basic feed, which is composed of the following ingredients in parts by weight: 65 parts of corn, 12 parts of soybean meal, 10 parts of bran, 10 parts of cottonseed meal, 3 parts of distiller's grains, 0.2 parts of sugarcane molasses, 0.1 parts of stone powder, and 0.1 parts of premix.

[0010] More preferably, the premix comprises vitamin A 17456 IU / kg, vitamin D3740 IU / kg, vitamin E 50 mg / kg, Fe 98.7 mg / kg, Zn 72.9 mg / kg, Mn 57.4 mg / kg, Cu 15.94 mg / kg, Se 0.33 mg / kg, I 1.30 mg / kg, and Co 0.39 mg / kg.

[0011] Beneficial effects:

[0012] Adding isochlorogenic acid to the feed of weaned lambs and controlling the added amount to 1.5 mg / g can significantly alleviate the weaning stress response of lambs, increase body weight, and reduce the feed-to-weight ratio. It has important promotion value in the lamb breeding industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0014] Figure 1 is the DPPH clearance rate of different samples.

[0015] Figure 2 This is the standard curve of FeSO4.

[0016] Figure 3 FRPA values ​​of different samples.

[0017] Figure 4 is the absorbance value of different samples.

[0018] Figure 5 is the hydroxyl radical scavenging rate of different samples.

[0019] Figure 6 is the hemolysis rate of different samples.

[0020] Figure 7 is the SOD activity of different samples.

[0021] Figure 8 is the MDA value of different samples.

[0022] Fig. 9 is the weaning diarrhea rate of lambs in the experimental groups with different isochlorogenic acid contents. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Example 1

[0025] Determination of antioxidant capacity of different natural extracts

[0026] Isochlorogenic acid is extracted from stevia and is a dicaffeoyl-substituted isomer of chlorogenic acid. This type of compound is an organic phenolic acid natural product formed by the condensation of quinic acid and caffeic acid through esterification reaction. Isochlorogenic acid has anti-inflammatory, antiviral, antihypertensive and in vitro antioxidant activities. Curcumin is a major active substance extracted from the dried rhizome of turmeric, a plant of the genus Curcuma in the ginger family. Curcumin has excellent antioxidant properties. β-Carotene is one of the carotenoids and is an orange-yellow fat-soluble compound. It is the most common and stable natural pigment in nature and also an excellent antioxidant.

[0027] The present invention measures the antioxidant capacity of the three natural extracts respectively, in order to seek a product that can effectively alleviate lamb weaning stress.

[0028] 1. Use 75% ethanol to prepare three antioxidants (see Table 1) into solutions with concentrations of 5, 10, 20, 40, 80, and 100 μg / mL, respectively.

[0029] Table 1

[0030]

[0031] The results obtained in this example were analyzed by one-way ANOVA using SPSS 25.0, and the results were expressed as mean ± SD. Duncan's multi-sided test was used to analyze the significant difference of the experimental means (p < 0.05). Origin 2022 was used to plot the analyzed data.

[0032] 2. Determination of DPPH clearance rate

[0033] Prepare DPPH ethanol solution (0.1mmol / L): weigh 3.94mg DPPH and add anhydrous ethanol to make up to 100mL in a brown volumetric flask, place in a cool place, and use it immediately after preparation.

[0034] Take 50 μL of sample solution and mix it with 100 μL of DPPH·ethanol solution, and react it at room temperature in the dark for 30 minutes. Measure the absorbance at 517 nm and record the result as sample absorbance (A 样 ). Use ethanol solution instead of sample solution as control tube, and perform the same treatment. The absorbance at 517nm is recorded as control absorbance (A 空 Each sample was measured three times in parallel and the average value was calculated. The DPPH scavenging ability of different pigment samples was calculated according to the following formula (see Table 2 and Appendix Figure 1 ).

[0035] DPPH clearance rate (%) = (A 空 -A 样 ) / A 空 *100%

[0036] Table 2 DPPH removal rate of natural extracts at different concentrations

[0037]

[0038] It can be seen that when the sample concentration was 100 μg / mL, the DPPH scavenging rate of the samples was tannic acid (82.87%) > isochlorogenic acid (60.91%) > curcumin (65.11%) > β-carotene (6.23%).

[0039] 3. Ferric ion reduction / FRAP assay

[0040] Hydrochloric acid solution (40mmol / L): add 0.1mL concentrated hydrochloric acid to 30mL with water.

[0041] TPTZ solution (10 mmol / L): weigh 31.233 mg of TPTZ and dilute to 10 mL with 40 mM hydrochloric acid.

[0042] Acetate buffer (0.3 mol / L, pH=3.6): 5.1 g of sodium acetate, add 20 mL of glacial acetic acid, dilute to 250 mL with water, place in a dark place for later use.

[0043] FRAP working solution: Acetate buffer (0.3 mol / L, pH=3.6) is fully mixed with TPTZ solution (10 mmol / L) and FeCl3 solution (20 mmol / L) in a ratio of 10:1:1, and preheated at 37°C. Take 300 μL of preheated reagent, add 30 μL of ultrapure water and 10 μL of sample solution, mix thoroughly, and react in a 37°C water bath in the dark for 30 minutes. Measure the absorbance at 593 nm and record the absorbance value (see Table 3). Prepare different concentrations of FeSO4:7H20 (5-1000 μmol / L), replace the sample solution for the above reaction, and use the corresponding absorbance values ​​to draw a standard curve of FeSO4 reducing power (see Appendix Figure 2 The ferric ion reducing / antioxidant capacity (FRAP) of different samples corresponding to FeSO4 was calculated by using the standard curve (see Table 4 and Appendix Figure 3 ). Each sample was measured three times in parallel and the average value was calculated.

[0044] Table 4 Absorbance values ​​after reaction at different FeSO4 concentrations

[0045]

[0046] Table 5 FRAP values ​​of samples with different concentrations

[0047]

[0048] It can be seen that when the sample concentration is 100 μg / mL, the FRAP values ​​of different samples are isochlorogenic acid (1096.89 FRAP) > curcumin (411.33 FRAP) > β-carotene (10.11 FRAP).

[0049] 4. Determination of total reducing power

[0050] Phosphate buffer (0.2 mol / L, pH 6.6): 14.32 g Na2HP04·12H20 and 24.96 g NaH2PO4·2H20 were diluted to 1 L with distilled water.

[0051] 1% Potassium ferrocyanide: Add 0.5 g of potassium ferrocyanide to 50 mL of water.

[0052] 0.1% ferric chloride: 0.05 g ferric chloride is diluted to 50 mL with water.

[0053] 10% trichloroacetic acid: 0.05 g trichloroacetic acid is diluted to 50 mL with water.

[0054] Take 1 mL of sample solution, add 1 mL of phosphate buffer and 1 mL of 1% potassium ferricyanide solution, mix thoroughly, and incubate in a 50°C water bath for 20 min. Then add 1 mL of 10% trichloroacetic acid solution, incubate at 25°C for 10 min, and then centrifuge at 8000 r / min for 10 min. Take 1 mL of supernatant, add 1 mL of distilled water and 0.5 mL of 0.1% ferric chloride solution, react for 10 min, and measure the absorbance at 700 nm (see Table 5 and Appendix). Figure 4 ). An equal volume of distilled water was used instead of the sample as a blank control. The higher the absorbance of the solution, the stronger the total reducing power. When the sample concentration was 100 μg / mL, the reducing power was isochlorogenic acid (0.671) > curcumin (0.272) > β-carotene (0.082).

[0055] Table 5 Total reducing power of samples with different concentrations

[0056]

[0057] 5. Determination of hydroxyl radical scavenging rate

[0058] 9mmol / L FeSO4: 0.25g FeSO4·7H20 is diluted to 100mL with deionized water.

[0059] 9mmol / L salicylic acid-ethanol: 0.1243g salicylic acid is diluted to 100mL with anhydrous ethanol.

[0060] 8.8 mol / L hydrogen peroxide: 89.89 mL 30% H202 diluted to 100 mL with water.

[0061] First add 1 mL of 9mmol / L FeSO4 to each EP tube, then add 1 mL of 9mmol / L salicylic acid-ethanol solution, 1 mL of each concentration sample, and 1 mL of 8.8mol / L hydrogen peroxide. Use distilled water instead of the sample as a blank control group. After reacting at 37℃ for 30 minutes, measure the sample group (A) at 510nm. 样 ) and blank control group (A 空 ) (see Table 6 and Appendix Figure 5 ).

[0062] Hydroxyl free radical scavenging rate = (A 空 -A 样 ) / A 空 *100%

[0063] Table 6 Hydroxyl radical scavenging rate of samples with different concentrations

[0064]

[0065] It can be seen that when the sample concentration is 100 μg / mL, the sample hydroxyl free radical scavenging rate is isochlorogenic acid (27.74%) > β-carotene (31.58%) > curcumin (28.88%).

[0066] 6. AAPH induces oxidative stress response in animal erythrocytes

[0067] PBS solution: 8.766 g NaCl, 0.680 g Na2HP04·12H20 and 1.2637 g NaH2PO4·2H20 were diluted to 1 L with distilled water.

[0068] AAPH (100mmol / L): 20.414g AAPH was diluted to 1L with PBS.

[0069] Take fresh animal blood, anticoagulate with heparin, centrifuge at 4℃2000r / min for 10min, discard the supernatant, and the resulting precipitate is red blood cells. Add an appropriate volume of PBS solution, gently blow away the animal red blood cells to form a uniform animal red blood cell suspension, then centrifuge at 3000r / min for 10min, and discard the supernatant. Repeat this process, wash the animal red blood cells with PBS solution 3 times. Finally, add an appropriate amount of PBS solution to prepare a 2% animal red blood cell suspension for use.

[0070] Sample group: Take 0.2 mL of red blood cell suspension and add 200 μL of sample.

[0071] AAPH group: 0.2 mL of red blood cell suspension was added to 200 μL of PBS solution.

[0072] Blank control group: 0.2 mL of red blood cell suspension was added to 200 μL of PBS solution, mixed with red blood cells, incubated at 37°C for 30 min with gentle shaking in the dark, then 200 μL of AAPH solution was added to the AAPH group and sample, and the blank control was added with an equal amount of PBS, mixed, and incubated for 4 h under the same conditions before measuring the experimental results. The experiment was designed to be repeated three times and the average was taken.

[0073] (1) Determination of AAPH-induced animal erythrocyte hemolysis rate

[0074] After the incubation, the reaction solution was centrifuged at 2000 r / min for 10 min at 4°C, and the absorbance value A at 540 nm of the supernatant was measured using an ELISA reader. The same concentration of red blood cell suspension was added with an equal volume of water for lysis, and the absorbance value B was also measured at 540 nm (results are shown in Table 7).

[0075] Hemolysis rate = A / B*100%

[0076] Table 7 Hemolysis rate of different samples at 100 μg / mL

[0077]

[0078] Through the study of the protective effect of different samples of the same concentration on the AAPH-induced erythrocyte hemolysis model, it was found that different samples had a protective effect on AAPH-induced erythrocyte hemolysis. Figure 6 As shown. Under the same incubation conditions, the blank group added PBS without AAPH treatment, the natural hemolysis rate was 9.32%, while the hemolysis rate of the AAPH group was 21.45%, which was significantly higher than that of the experimental group treated with isochlorogenic acid and curcumin (p<0.01). After β-carotene treatment, the hemolysis rate was 21.13%, which was not significantly different from the experimental group treated with AAPH.

[0079] (2) Determination of SOD activity and malondialdehyde (MDA) level in AAPH-induced animal erythrocytes

[0080] Take an appropriate amount of pretreated red blood cells, add PBS buffer to wash 2 to 3 times, add about 4 times the volume of ultrapure water to lyse the cells, and centrifuge at 3000r / min, 4℃ for 5min after ice bath; after centrifugation, take the supernatant and store it in a -20℃ refrigerator.

[0081] The SOD activity (no.A001 Nanjing Jiancheng Bioengineering Institute) and MDA (no.A003 Nanjing Jiancheng Bioengineering Institute) levels were measured. The hemoglobin content in animal erythrocytes was measured using a commercial kit (no.C021 Nanjing Jiancheng Bioengineering Institute). The results of animal erythrocyte SOD were expressed as enzyme activity units per gram of hemoglobin (U / gHb) (see Table 8 and Appendix Figure 7), the results of MDA were expressed as the level of MDA per gram of hemoglobin (mmol / g: Hb) (see Table 9 and Appendix Figure 8 ).

[0082] Table 8 100μg / mL SOD of different samples

[0083]

[0084] Table 9 100 μg / mL MDA of different samples

[0085]

[0086] In the erythrocyte oxidative hemolysis model, free radicals attacking cells will cause lipid peroxidation. Malondialdehyde (MDA) is one of the byproducts of lipid peroxidation. The content of MDA is used as a biomarker for the stress reaction process. After treatment, the APPH group was significantly higher than the experimental group treated with isochlorogenic acid and curcumin (p<0.01). After treatment with β-carotene, the MDA content was 5.46%, which was not significantly different from the MDA content of 5.59% in the AAPH group.

[0087] Example 2

[0088] Isochlorogenic acid relieves weaning stress in lambs

[0089] This experiment was conducted at Baosen Animal Husbandry Co., Ltd., Cangzhou City, Hebei Province. Sixty male Hu sheep lambs aged 21 days were selected for the experiment, with an average initial weight of (8.5±1.0) kg. A single-factor experimental design was used, and the lambs were randomly divided into four treatments according to the principle of similar weight, with 15 replicates in each treatment and one lamb in each replicate. The control group (CON) was fed a basic diet, and the experimental groups (experimental group I, experimental group II, and experimental group III) were supplemented with 1 mg / g, 1.5 mg / g, and 2.0 mg / g of stevia isochlorogenic acid (extracted from stevia, provided by Hebei Chenguang Biotechnology Group Co., Ltd.) in the basic diet. The lambs were weaned on the 35th day as the 0th day, and the experiment started on the -14th day (14 days before weaning). They were breastfed with the mother and fed with the starter feed (basic diet). After weaning, the ewes were removed and continued to be fed with the starter feed. The feeding experiment ended at 14d (14 days) after weaning.

[0090] Basic feed: 65 parts of corn, 12 parts of soybean meal, 10 parts of bran, 10 parts of cottonseed meal, 3 parts of distiller's grains, 0.2 parts of sugarcane molasses, 0.1 parts of stone powder, 0.1 parts of premix (vitamin A 17456IU / kg, vitamin D3740 IU / kg, vitamin E 50mg / kg, Fe98.7mg / kg, Zn 72.9mg / kg, Mn 57.4mg / kg, Cu 15.94mg / kg, Se 0.33mg / kg, I1.30 mg / kg, Co0.39mg / kg).

[0091] Table 10 Nutritional composition of basic feed

[0092]

[0093]

[0094] The starter feed was fed at 6:00 am and 17:00 pm every day, and the animals were allowed to eat and drink freely. During the experiment, the average daily feed intake (ADFI), average daily gain (ADG), feed-to-weight ratio, diarrhea rate were recorded, and blood was collected regularly to test biochemical indicators.

[0095] The experimental data of this example were first summarized and sorted using Excel 2019, and the ANOVA process in SPSS23.0 software was used for one-way analysis of variance, and the Duncan method was used for multiple comparisons of the mean values. Linear and quadratic regression were used to analyze the dose effect of isochlorogenic acid, and the time and time treatment interaction were analyzed for differences. P<0.05 indicates a significant difference.

[0096] (1) During the experiment, the daily feed intake of each group of lambs was recorded. The lambs were weighed on an empty stomach at the beginning of the experiment and on the day of weaning (day 0), 3 days after weaning, 7 days after weaning, and 14 days after weaning to calculate the average daily feed intake (ADFI), average daily weight gain (ADG), and feed-to-weight ratio (see Table 11).

[0097] Table 11 Effects of isochlorogenic acid on growth performance of weaned lambs

[0098]

[0099] It can be seen that the weight of lambs increased significantly after weaning, and the average daily feed intake also gradually increased. Time and the interaction between time and treatment had a significant effect on the growth performance of lambs. The feed-to-weight ratio of the experimental group was significantly reduced compared with the control group.

[0100] Lamb weight, average daily weight gain, and average daily feed intake are all indicators closely related to lamb growth performance, which can reflect the growth rate of lambs over a period of time. Feed-to-weight ratio is an important indicator used to measure feed conversion rate. The test results show that 1.5 mg / g of isochlorogenic acid can promote the growth of lambs, probably because it belongs to the phenolic acid substances, which can promote the cultivation of acidic environment in the rumen and improve the digestion ability of lambs.

[0101] (2) Diarrhea rate

[0102] On the day of weaning (day 0), on the 3rd, 7th and 14th days before morning feeding, collect lamb feces, observe the condition of lamb feces and accurately record the number of lambs with diarrhea (see Appendix Fig. 9 ).

[0103] As can be seen from the attached figure, the diarrhea rate of test group II was the lowest on the day of lamb weaning, which was significantly lower than that of the control group and other groups. On the 3rd day after weaning, there was an increase in the diarrhea rate of each group, and the increase rate of test group II was 17%, which was significantly lower than that of the control group. On the 7th day after weaning, the diarrhea rate of each group showed a downward trend, with the lowest being 8% in test groups II and test groups III. On the 14th day after weaning, the diarrhea rate of test group II was 0%, which was significantly lower than that of other groups.

[0104] (3) Blood sample collection

[0105] On the day of weaning (day 0), day 3, day 7 and before morning feeding on day 14, the lambs were tied up and blood was collected through the jugular vein. The blood collection tube was tilted at 60 degrees at room temperature and allowed to stand for 20 minutes. It was then centrifuged at 3500g for 3 minutes to separate the supernatant, dispensed into 1.5ml centrifuge tubes, and stored in a -20℃ refrigerator for later use.

[0106] Serum biochemical indicators: total protein (TP), albumin (ALB), globulin (GLB), creatinine (CR), urea (BUN). The above indicators were detected by automatic biochemical analyzer (see Table 12).

[0107] Serum antioxidant indexes: catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-COA), glutathione peroxidase (GSH-Px). The above indexes were detected by the kit of Nanjing Jiancheng Bioengineering Research Institute, and the operation was carried out according to the instructions of the kit (see Table 13).

[0108] Serum immune indicators: immunoglobulin A (Ig-A), immunoglobulin G (Ig-G), immunoglobulin M (Ig-M), tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), interleukin-6 (IL-6). The above indicators were measured using the enzyme-linked immunosorbent assay kit of Jiangsu Enzyme Absorption Industry Co., Ltd., and the operation was carried out according to the instructions of the kit (see Table 14).

[0109] Intestinal permeability indexes: diamine oxidase (DAO), D-lactic acid (D-LA), endotoxin (ET), cortisol (COR). The above indexes were all measured using the enzyme-linked immunosorbent assay kit of Jiangsu Enzyme Immunity Industry Co., Ltd., and the operation was carried out according to the instructions of the kit (see Table 15).

[0110] Table 12 Effects of isochlorogenic acid on serum biochemical parameters of weaned lambs

[0111]

[0112] Various biochemical indicators in serum can indirectly reflect the health status of lambs. The results showed that the addition of isochlorogenic acid had no significant effect on the total protein and creatinine content of lambs, but time had a significant effect on the globulin and creatinine content, and the albumin content was significantly different between different groups at 14 days after weaning. In the late weaning period, the globulin content in the isochlorogenic acid-added group was significantly lower than that in the control group, which may be because isochlorogenic acid can improve the immunity of lambs.

[0113] Table 13 Effects of isochlorogenic acid on serum antioxidant indexes of weaned lambs

[0114]

[0115] When lambs are weaned, the sudden change from lamb milk to lamb feed causes the lambs to not build up enough tolerance, which results in oxidative stress. SOD and CAT are the body's first line of defense against oxygen free radicals. Lipid peroxidation produces MDA, and T-AOC can directly reflect the body's antioxidant capacity. CAT, SOD, and T-AOC all show a trend of first decreasing and then increasing. This may be because the oxidative balance in the lambs' bodies is broken due to weaning stress, and the excessive free radicals produced cannot be cleared in time. As the weaning time increases, the lambs' bodies gradually recover. At 3 days after weaning, the lambs' stress response is the strongest, lipid oxidation is the most serious, and therefore the MDA content is the highest.

[0116] Table 14 Effects of isochlorogenic acid on serum immune parameters of weaned lambs

[0117]

[0118] Immunoglobulins are important antibodies produced when the body is damaged and triggers immune responses. Cytokines can regulate immune responses to maintain the health of the body. According to their different functions, they can be divided into pro-inflammatory factors (TNF-a, IL-1β, IL-6) and anti-inflammatory factors (IgA, IgG, IgM). Immunoglobulin and cytokine levels are important indicators for evaluating immune responses. The results show that the immunoglobulin content of the experimental group with added isochlorogenic acid increased significantly, and the pro-inflammatory factor content decreased significantly, indicating that adding isochlorogenic acid to the diet of weaned lambs can effectively improve the body's immune function.

[0119] Table 15 Effect of isochlorogenic acid on intestinal permeability of weaned lambs

[0120]

[0121] The DAO content was significantly lower than that of the control group at 3 days after weaning. During the experiment, the D-LA content of the experimental group was significantly lower than that of the control group, and the content of group II was significantly lower than that of other groups. The interaction between time and time and treatment had a significant effect on the content of DAO and D-LA.

[0122] When the intestine is damaged, D-LA produced by bacterial metabolism and lysis will accumulate excessively, and DAO present in the intestinal villus epithelial cells can pass through the intestinal mucosal barrier into the blood, and the COR content will increase. In the early stage of the experiment, due to oxidative stress, the lamb intestine was damaged, and the DAO and D-LA contents increased, but the contents in the experimental group were lower than those in the control group, indicating that isochlorogenic acid can alleviate intestinal damage and promote the integrity of the intestinal barrier.

[0123] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0124] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A natural plant extract feed additive for relieving lamb weaning stress, characterized in that: The additive is isochlorogenic acid, and its added amount in the feed is 1.5 mg / g.

2. The use of isochlorogenic acid in preparing feed for alleviating lamb weaning stress, characterized in that: The amount of isochlorogenic acid added to the basic feed is 1.5 mg / g.

3. A feed for alleviating lamb weaning stress, characterized in that: The feed includes 1.5 mg / g of isochlorogenic acid.

4. The feed for alleviating lamb weaning stress according to claim 3, characterized in that: The feed also includes a basic feed, which is composed of the following ingredients in parts by weight: 65 parts of corn, 12 parts of soybean meal, 10 parts of bran, 10 parts of cottonseed meal, 3 parts of distiller's grains, 0.2 parts of sugarcane molasses, 0.1 parts of stone powder, and 0.1 parts of premix.

5. The feed for alleviating lamb weaning stress according to claim 4, characterized in that: The premix contains 17456 IU / kg of vitamin A, 3740 IU / kg of vitamin D, 50 mg / kg of vitamin E, 98.7 mg / kg of Fe, 72.9 mg / kg of Zn, 57.4 mg / kg of Mn, 15.94 mg / kg of Cu, 0.33 mg / kg of Se, 1.30 mg / kg of I and 0.39 mg / kg of Co.

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