Citrus chachiensis submicron powder with anti-inflammatory effect and preparation method thereof

Abstract

The invention discloses Citrus chachiensis submicron powder with an anti-inflammatory effect and a preparation method of the Citrus chachiensis submicron powder. The Citrus chachiensis submicron powder comprises the following raw material components in parts by mass: 15-30 parts of Citrus chachiensis dried orange peel powder, 30-50 parts of Citrus chachiensis pulp powder and 4-8 parts of Citrus chachiensis seed powder. The optimal anti-inflammatory effect is achieved through efficient compounding of different parts of Citrus chachiensis, efficient utilization of whole fruits is achieved, environmental pollution and resource waste are reduced, and economic benefits are improved. The preparation method comprises the following steps: sequentially carrying out high-voltage pulse electric field puffing, differential pressure puffing and vacuum freeze drying on the fruit body, separating pulp from fruit seeds, respectively carrying out dry-method low-temperature superfine grinding on the citrus chachiensis submicron powder, the pulp and the fruit seeds, and compounding according to the raw material components to obtain the citrus chachiensis submicron powder with the anti-inflammatory effect. According to the method disclosed by the invention, through the synergistic effect of multiple treatment technologies, the dissolution of functional active substances such as anti-inflammatory components is remarkably improved, and the Citrus chachiensis submicron powder with a good anti-inflammatory effect is obtained by combining with component compounding.

Description

Technical Field

[0001] The invention belongs to the technical field of food and its processing, and relates to a tea citrus superfine powder with anti-inflammatory effect and a preparation method thereof, and specifically relates to a pure natural tea citrus whole fruit superfine powder prepared by taking tangerine peel, tea citrus pulp and fruit seeds as raw materials without adding any food additives and a preparation method thereof. Background Art

[0002] Food powder is a dry, fine solid material that can be found everywhere in people's daily lives and in the food industry. Food powder has many advantages and economical advantages due to its reduced volume / weight, easy packaging, handling and transportation, improved bioaccessibility and greater nutritional value. More importantly, many food raw materials are in solid (especially fresh food raw materials, such as fruits and vegetables) or liquid state, which are easily degraded and decomposed in the natural environment. The corresponding powders are low in water content and easy to store, which can better maintain the stability of food raw materials and facilitate further utilization. Inflammation is a physiological response caused by tissue trauma, bleeding, or infection with pathogenic bacteria. Excessive and persistent inflammatory responses are usually associated with many diseases. In addition, inflammation is also believed to be related to obesity and metabolic disorders. Eating anti-inflammatory foods can improve inflammatory responses to a certain extent.

[0003] Tea-branch citrus is the authentic raw material of Guang Chenpi, a traditional Chinese medicinal material specified in the Chinese Pharmacopoeia. Tea-branch citrus fruit is rich in nutrients, including organic acids, polysaccharides and other nutrients, as well as functional compounds such as flavonoids and polyphenols. The types and contents of active ingredients in different parts of tea-branch citrus are significantly different, and their physiological effects are also different. Tea-branch citrus peel can be divided into three stages due to different harvesting times, namely green citrus, two red citrus, and big red citrus. The main active substances in tea-branch citrus peel are volatile oils and flavonoids, and the main effects are regulating qi and relieving stagnation, replenishing qi and strengthening the spleen, and drying dampness and resolving phlegm; tea-branch citrus pulp contains rich functional active ingredients, such as flavonoids, polysaccharides, vitamins, dietary fiber, etc., and has excellent nutritional value and health care functions. The main active ingredients of tea-branch citrus kernels are limonin and its analogues, and the higher content of ingredients is limonin and nomilin, which has multiple pharmacological activities such as anti-free radicals, anti-inflammatory analgesia, and anti-tumor. Different parts of the tea-branch citrus have different types of functional active ingredients and have extremely high nutritional value. However, most of the tea-branch citrus fruits on the market are not fully utilized. Generally, only the peel of the tea-branch citrus fruit is used to make tangerine peel, and the pulp and core are mostly treated as waste. This neither makes better use of the tea-branch citrus fruit resources nor conforms to the "green" development concept. In view of the above shortcomings, no researchers have proposed relevant solutions in the prior art. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a tea branch orange ultrafine powder with good anti-inflammatory effect, full fruit resource utilization, zero additives and anti-inflammatory efficacy and a preparation method thereof.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] A tea citrus superfine powder with anti-inflammatory effect, the tea citrus superfine powder comprises the following raw material components by weight:

[0007] 15 to 30 portions of tea branch orange peel powder,

[0008] The content of nobiletin in the tea branch orange peel powder is 3.61 mg / g to 4.32 mg / g;

[0009] 30 to 50 portions of tea branch orange pulp powder,

[0010] The content of hesperidin in the tea branch orange pulp powder is 8.05mg / g to 9.65mg / g;

[0011] 4 to 8 portions of tea branch citrus fruit seed powder,

[0012] The content of limonin in the tea branch citrus fruit seed powder is 18.12 mg / g to 19.05 mg / g.

[0013] The above-mentioned tea citrus superfine powder with anti-inflammatory effect is further preferably composed of the following raw material components by weight:

[0014] 20 portions of tea branch orange peel powder,

[0015] 40 portions of tea branch orange pulp powder,

[0016] 6 parts of Citrus aurantium seed powder.

[0017] The above-mentioned tea citrus superfine powder with anti-inflammatory effect is further preferably composed of the following raw material components by weight:

[0018] 15 portions of tea branch orange peel powder,

[0019] 30 portions of tea branch orange pulp powder,

[0020] 4 parts of Citrus aurantium seed powder.

[0021] The above-mentioned tea citrus superfine powder with anti-inflammatory effect is further preferably composed of the following raw material components by weight:

[0022] 30 portions of tea branch orange peel powder,

[0023] 50 portions of tea branch orange pulp powder,

[0024] 8 parts of Citrus aurantium seed powder.

[0025] The above-mentioned tea-branch orange ultrafine powder with anti-inflammatory effect is further preferably that the tea-branch orange is a big red orange, and the big red orange is harvested from November to December every year.

[0026] The above-mentioned tea-twig citrus ultrafine powder with anti-inflammatory effect is further preferably prepared from tea-twig citrus tangerine peel powder which is more than three years old.

[0027] The above-mentioned tea-branch citrus ultrafine powder with anti-inflammatory effect is further preferably that the pulp used in the tea-branch citrus pulp powder includes pulp body, citrus skin and tangerine peel.

[0028] As a general inventive concept, the present invention also provides a method for preparing the above-mentioned tea branch orange ultrafine powder with anti-inflammatory effect, comprising the following steps:

[0029] S1, separating the peel and the fruit body of the tea-branch orange, the peel is used to make the tea-branch orange tangerine peel, and the fruit body is subjected to a high-voltage pulse electric field treatment, the electric field strength is 10kV / cm~30kV / cm, and the treatment time is 10min~30min;

[0030] S2, subjecting the tea-branch citrus fruit body treated in step S1 to differential pressure puffing treatment, wherein the puffing pressure is 0.05MPa-0.1MPa, the puffing temperature is 35°C-40°C, and the puffing time is 0.5h-1h;

[0031] S3, subjecting the tea-branch orange fruit body treated in step S2 to vacuum freeze-drying;

[0032] S4, separating the pulp and seeds of the tea-branch citrus fruit body treated in step S3 to obtain tea-branch citrus pulp and tea-branch citrus seeds;

[0033] S5, respectively grinding the tea-branch orange peel, tea-branch orange pulp and tea-branch orange seeds by low-temperature dry ultrafine grinding technology to obtain tea-branch orange peel powder, tea-branch orange pulp powder and tea-branch orange seed powder;

[0034] S6. Compound the tangerine peel powder, tangerine pulp powder and tangerine seed powder according to a certain proportion to obtain tangerine ultrafine powder with anti-inflammatory effect.

[0035] The above-mentioned method for preparing the tea-branch orange ultrafine powder with anti-inflammatory effect is further preferred. In step S3, the vacuum freeze-drying treatment is to pre-freeze the tea-branch orange fruit body at -25℃ to -30℃ for 3h to 5h, and then dry it under vacuum conditions at -25℃ to -20℃ for 20h to 25h.

[0036] The method for preparing the above-mentioned Citrus aurantium ultrafine powder with anti-inflammatory effect is further preferably that in step S5, the pulverizing temperature is 0°C to 4°C, and the pulverizing time is 10min to 30min.

[0037] The method for preparing the above-mentioned tea citrus ultrafine powder with anti-inflammatory effect is further preferred. In step S6, the tea citrus ultrafine powder is irradiated and sterilized for 30 minutes to 40 minutes, and then the sterilized ultrafine powder is vacuum-packed with aluminum foil or tin foil.

[0038] Compared with the prior art, the advantages of the present invention are:

[0039] (1) The present invention aims to obtain a pure natural compound whole-fruit superfine powder with anti-inflammatory effect without any additives. Through the present research practice, it is found that different parts of the tea-twig citrus contain different anti-inflammatory active ingredients, among which the main ingredient in the tea-twig tangerine peel powder that has an anti-inflammatory effect is nobiletin, the main ingredient in the tea-twig citrus pulp powder that has an anti-inflammatory effect is hesperidin, and the main ingredient in the tea-twig citrus seed powder that has an anti-inflammatory effect is limonin, and different raw material ratios have a great influence on the anti-inflammatory activity of the tea-twig citrus superfine powder. The tea-twig citrus whole-fruit superfine powder with anti-inflammatory effect of the present invention has specific requirements for the content of anti-inflammatory ingredients in the tea-twig tangerine peel powder, the tea-twig citrus pulp powder, and the tea-twig citrus seed powder and the raw material ratio, and includes 15 to 30 parts of tea-twig citrus tangerine peel powder, 30 to 50 parts of tea-twig citrus pulp powder, and 4 to 8 parts of tea-twig citrus seed powder in terms of mass parts. According to the specific ratio, citrus tangerine peel powder with a nobiletin content of 3.61 mg / g to 4.32 mg / g, citrus tangerine pulp powder with a hesperidin content of 8.05 mg / g to 9.65 mg / g, and citrus tangerine seed powder with a limonin content of 18.12 mg / g to 19.05 mg / g are used. The compounded citrus tangerine whole fruit superfine powder has excellent anti-inflammatory efficacy and rich nutrients, and its anti-inflammatory effect is far superior to that of single tangerine peel powder. By establishing a rat colitis inflammation model experiment, it is found that the compounded whole fruit superfine powder can promote the recovery of rat colon inflammation and significantly increase the length of rat colon, while the anti-inflammatory efficacy of the citrus tangerine whole fruit superfine powder obtained according to the ratio other than that specified in the present invention is significantly reduced.

[0040] (2) The present invention uses a specific ratio of raw materials to form an embedding effect using polysaccharides such as pectin in tangerine peel and capsule, which is beneficial to the delivery of effective ingredients in the body. The specific ratio of compounding can solve the stability problem of the product without using additives. Specifically, the tangerine pulp powder has high sugar and acid contents, containing sucrose, fructose, and glucose in an amount of 27.1 g / 100 g, 10.7 g / 100 g, and 9.3 g / 100 g, respectively. The organic acid is mainly citric acid, with a content of 2.4 g / kg. However, foods with high sugar and acid contents will form a viscous structure with high hygroscopicity after dehydration, so the pulp powder is easy to absorb moisture. In order to solve this problem, high molecular weight solutes such as maltodextrin are generally added to improve its stability. The fiber component in tangerine peel can replace the processing aids starch, maltodextrin, etc. added in the production process. Compounding with tangerine peel powder can solve the problems of easy moisture absorption and caking, thereby improving its stability and facilitating storage and sales. At the same time, the invention also realizes the efficient resource utilization of the whole fruit of the tea-branch citrus through the compound design, and converts the pulp and core of the tea-branch citrus, which are usually treated as waste in the industry, into economic benefits, and has broad market prospects.

[0041] (2) The preparation method of the tea citrus ultrafine powder with anti-inflammatory effect of the present invention adopts high-voltage pulse electric field, differential pressure puffing and vacuum freeze drying to greatly increase the content of anti-inflammatory components in the ultrafine powder and improve the drying efficiency. Compared with the method of using only vacuum freeze drying, or only using high-voltage pulse electric field or differential pressure puffing and vacuum freeze drying, the content of nobiletin in the tea citrus tangerine peel powder obtained by the coordinated treatment of the three is 3.61mg / g~4.32mg / g; the content of hesperidin in the tea citrus pulp powder is 8.05mg / g~9.65mg / g; the content of limonin in the tea citrus seed powder is 18.12mg / g~19.05mg / g. In comparison, the content of nobiletin in the tea citrus tangerine peel processed by the prior art is about 2.5mg / g, the content of hesperidin in the pulp is about 6.5mg / g, and the content of limonin in the fruit seed is about 15mg / g, which is significantly lower than that of the present invention. At the same time, total phenol and vitamin C also have anti-inflammatory effects. The contents of total phenol and vitamin C in the ultrafine powder of the present invention are respectively increased by 12% and 14% compared with comparative example 1, and the drying efficiency is increased by 24% to 37% compared with comparative example 4, which significantly improves the anti-inflammatory effect of the ultrafine powder and saves energy. Among them, the high-voltage pulse electric field and pressure difference puffing change the cell structure of the raw material, on the one hand, to improve the dissolution rate of functional active ingredients such as anti-inflammatory ingredients, and on the other hand, to improve the drying efficiency; and vacuum freeze drying is carried out at low temperature, which is conducive to retaining the biological activity of the anti-inflammatory ingredients in the raw material. Further, low-temperature ultrafine grinding is adopted, and the processing process continues to maintain a low temperature, which can better reduce the loss of anti-inflammatory ingredients compared with ordinary ultrafine grinding, and prolong the stability of the product while retaining the anti-inflammatory ingredients in the product to the maximum extent. The present invention can promote the dissolution of anti-inflammatory components through high-voltage pulse electric field, pressure difference puffing and vacuum freeze drying, and obtain the best drying efficiency, so that the prepared tea citrus superfine powder has the best anti-inflammatory component dissolution rate, retains its nutrients to a large extent, and further promotes the anti-inflammatory effect of the product, and combined with the optimal compounding ratio, the tea citrus whole fruit superfine powder with the best anti-inflammatory effect is obtained. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is an electron microscope image of the Citrus aurantium ultrafine powder with anti-inflammatory effect according to Example 1 of the present invention.

[0043] Figure 2 It is a liquid chromatogram of the main anti-inflammatory components in the tea-branch citrus pulp powder, tea-branch citrus tangerine peel powder, and tea-branch citrus seed powder in Example 1 of the present invention, wherein Figure A is a liquid chromatogram of hesperidin in the tea-branch citrus pulp powder; Figure B is a liquid chromatogram of nobiletin in the tea-branch citrus tangerine peel powder; and Figure C is a liquid chromatogram of limonin in the tea-branch citrus seed powder.

[0044] Figure 3 This is a color comparison diagram of the tea branch orange superfine powder of Example 1 and Comparative Examples 7 and 9 of the present invention.

[0045] Figure 4 It is a comparison chart of total phenol content, vitamin C content, and antioxidant capacity of Example 1 of the present invention and Comparative Examples 1, 2, 3, 7, and 9.

[0046] Figure 5 The figure is a comparison chart of the test results of the rat colitis inflammation model of Example 1 of the present invention and various comparative examples. DETAILED DESCRIPTION

[0047] The present invention is further described below in conjunction with the accompanying drawings and specific preferred embodiments, but the protection scope of the present invention is not limited thereby.

[0048] In the following examples of the present invention, unless otherwise specified, the materials and instruments used are commercially available, the equipment used is conventional equipment, and the data obtained are the average values ​​of more than three repeated experiments.

[0049] Embodiment 1:

[0050] A citrus tangerine ultrafine powder with anti-inflammatory effect comprises the following raw material components by weight: 20 parts of citrus tangerine peel powder, 30 parts of citrus tangerine pulp powder and 4 parts of citrus tangerine seed powder; wherein the content of nobiletin in the citrus tangerine peel powder is 4.32 mg / g, the content of hesperidin in the citrus tangerine pulp powder is 9.65 mg / g, and the content of limonin in the citrus tangerine seed powder is 19.05 mg / g.

[0051] Among them, the tea-branch tangerine is a big red tangerine that is harvested in December; the tea-branch tangerine tangerine peel powder is made from the tangerine peel of tea-branch tangerine that is more than three years old; the pulp used in the tea-branch tangerine pulp powder includes the pulp body, citrus skin and tangerine peel.

[0052] A method for preparing the tea branch orange superfine powder with anti-inflammatory effect in the above embodiment specifically comprises the following steps:

[0053] S1, separating the peel of the tea-branch orange from the fruit body, the peel is used to make the tea-branch orange dried tangerine peel, and the fruit body is treated with a high-voltage pulse electric field with an electric field strength of 20 kV / cm for 10 min;

[0054] S2, subjecting the tea-branch citrus fruit body treated in step S1 to a pressure differential puffing treatment, wherein the puffing pressure is 0.05 MPa, the puffing temperature is 40° C., and the puffing time is 1 h;

[0055] S3, subjecting the tea-branch citrus fruit body treated in step S2 to a vacuum freeze-drying treatment, i.e., pre-freezing at -25°C for 5 hours, and then drying at -25°C under vacuum conditions for 25 hours;

[0056] S4, separating the pulp and seeds of the tea-branch citrus fruit body treated in step S3 to obtain tea-branch citrus pulp and tea-branch citrus seeds;

[0057] S5, respectively crushing the tea branch orange peel, tea branch orange pulp and tea branch orange fruit seeds by low temperature dry ultrafine grinding technology to obtain tea branch orange peel powder, tea branch orange pulp powder and tea branch orange fruit seed powder, the crushing temperature is 4 ° C, and the crushing time is 10min;

[0058] S6. Compound the tangerine peel powder, tangerine pulp powder and tangerine seed powder according to a certain proportion to obtain tangerine ultrafine powder with anti-inflammatory effect.

[0059] In step S6, the compounded tea branch orange ultrafine powder is sent to an irradiation room for irradiation sterilization for 30 minutes, and then the sterilized ultrafine powder is vacuum-packed with aluminum foil or tin foil.

[0060] The ultrafine powder of this embodiment was subjected to electron microscope scanning, and the result is as follows Figure 1 As shown, from Figure 1 It can be seen that the average particle size of the ultrafine powder obtained under the pulverizing conditions of this embodiment is <20 μm. The pulverizing conditions and the average pulverizing particle size can better ensure the optimal dissolution of the effective ingredients in the tea branch orange ultrafine powder, such as ingredients with anti-inflammatory effects and nutrients.

[0061] Liquid chromatography was used to detect the anti-inflammatory components in the tea-twig citrus pulp powder, tea-twig citrus tangerine peel powder, and tea-twig citrus fruit seed powder of the present embodiment, respectively. The content of nobiletin in the tea-twig citrus tangerine peel powder was 4.32 mg / g, the content of hesperidin in the tea-twig citrus pulp powder was 9.65 mg / g, and the content of limonin in the tea-twig citrus fruit seed powder was 19.05 mg / g. The results are as follows Figure 2 As shown, Figure 2 The liquid chromatograms of the main anti-inflammatory components in the tea-twig citrus pulp powder, tea-twig citrus tangerine peel powder, and tea-twig citrus seed powder in Example 1 are shown in Figure A, wherein Figure A is a liquid chromatogram of hesperidin in the tea-twig citrus pulp powder; Figure B is a liquid chromatogram of nobiletin in the tea-twig citrus tangerine peel powder; Figure C is a liquid chromatogram of limonin in the tea-twig citrus seed powder. Figure 2 It can be seen that there are significant differences in the main anti-inflammatory ingredients in citrus fruit pulp powder, tangerine peel powder and fruit seed powder, and they need to be efficiently compounded to achieve better anti-inflammatory effects.

[0062] Embodiment 2:

[0063] A citrus tangerine ultrafine powder with anti-inflammatory effect comprises the following raw material components by weight: 20 parts of citrus tangerine peel powder, 50 parts of citrus tangerine pulp powder and 8 parts of citrus tangerine seed powder; wherein the content of nobiletin in the citrus tangerine peel powder is 4.02 mg / g, the content of hesperidin in the citrus tangerine pulp powder is 9.33 mg / g, and the content of limonin in the citrus tangerine seed powder is 18.85 mg / g.

[0064] Among them, the tea-branch tangerine is a big red tangerine that is harvested in December; the tea-branch tangerine tangerine peel powder is made from the tangerine peel of tea-branch tangerine that is more than three years old; the pulp used in the tea-branch tangerine pulp powder includes the pulp body, citrus skin and tangerine peel.

[0065] A method for preparing the tea branch orange superfine powder with anti-inflammatory effect in the above embodiment specifically comprises the following steps:

[0066] S1, separating the peel of the tea-branch orange from the fruit body, the peel is used to make the tea-branch orange dried tangerine peel, and the fruit body is treated with a high-voltage pulse electric field with an electric field strength of 20 kV / cm for 10 min;

[0067] S2, subjecting the tea-branch citrus fruit body treated in step S1 to a pressure differential puffing treatment, wherein the puffing pressure is 0.1 MPa, the puffing temperature is 35° C., and the puffing time is 0.5 h;

[0068] S3, subjecting the tea-branch citrus fruit body treated in step S2 to a vacuum freeze-drying treatment, i.e., pre-freezing at -25°C for 5 hours, and then drying at -25°C under vacuum conditions for 20 hours;

[0069] S4, separating the pulp and seeds of the tea-branch citrus fruit body treated in step S3 to obtain tea-branch citrus pulp and tea-branch citrus seeds;

[0070] S5, respectively crushing the tea branch orange peel, tea branch orange pulp and tea branch orange fruit seeds by low temperature dry ultrafine grinding technology to obtain tea branch orange peel powder, tea branch orange pulp powder and tea branch orange fruit seed powder, the crushing temperature is 2 ° C, and the crushing time is 20min;

[0071] S6. Compound the tangerine peel powder, tangerine pulp powder and tangerine seed powder according to a certain proportion to obtain tangerine ultrafine powder with anti-inflammatory effect.

[0072] In step S6, the compounded tea branch orange ultrafine powder is sent to an irradiation room for irradiation sterilization for 30 minutes, and then the sterilized ultrafine powder is vacuum-packed with aluminum foil or tin foil.

[0073] Liquid chromatography was used to detect the anti-inflammatory components in the tea-branch citrus pulp powder, tea-branch citrus tangerine peel powder, and tea-branch citrus fruit seed powder of the present embodiment, respectively. The content of nobiletin in the tea-branch citrus tangerine peel powder was 4.02 mg / g, the content of hesperidin in the tea-branch citrus pulp powder was 9.33 mg / g, and the content of limonin in the tea-branch citrus fruit seed powder was 18.85 mg / g.

[0074] Embodiment 3:

[0075] The invention discloses an ultrafine powder of citrus aurantium with anti-inflammatory effect, which comprises the following raw material components by weight: 30 parts of citrus aurantium tangerine peel powder, 50 parts of citrus aurantium pulp powder and 8 parts of citrus aurantium fruit seed powder, wherein the content of nobiletin in the citrus aurantium tangerine peel powder is 4.12 mg / g, the content of hesperidin in the citrus aurantium pulp powder is 9.23 mg / g, and the content of limonin in the citrus aurantium fruit seed powder is 18.95 mg / g.

[0076] Among them, the tea-branch tangerine is a big red tangerine that is harvested in December; the tea-branch tangerine tangerine peel powder is made from the tangerine peel of tea-branch tangerine that is more than three years old; the pulp used in the tea-branch tangerine pulp powder includes the pulp body, citrus skin and tangerine peel.

[0077] A method for preparing Citrus aurantium ultrafine powder with anti-inflammatory effect, wherein the specific steps are the same as those in Example 1.

[0078] The tea branch orange ultrafine powder with anti-inflammatory effect prepared in Example 2 and Example 3 has the same or similar technical effects as Example 1.

[0079] Comparative Example 1:

[0080] A composite tea citrus superfine powder is prepared by using the tea citrus tangerine peel powder, tea citrus pulp powder and tea citrus seed powder obtained in steps S1-S5 of the preparation method of Example 1 according to step S6. The composite tea citrus superfine powder of this comparative example is different in that the amount of tea citrus tangerine peel powder in the composite tea citrus superfine powder of this comparative example is reduced. The specific raw material components and effects are compared, as shown in Table 1.

[0081] Comparative Example 2:

[0082] A composite tea citrus superfine powder is prepared by using the tea citrus tangerine peel powder, tea citrus pulp powder and tea citrus seed powder obtained in steps S1-S5 of the preparation method of Example 1 according to step S6. The composite tea citrus superfine powder of this comparative example is different in that the amount of tea citrus pulp powder in the composite tea citrus superfine powder of this comparative example is reduced. The specific effects are compared, as shown in Table 1.

[0083] Comparative Example 3:

[0084] A composite tea citrus superfine powder is prepared by using the tea citrus tangerine peel powder, tea citrus pulp powder and tea citrus seed powder obtained in steps S1-S5 of the preparation method of Example 1 according to step S6. The composite tea citrus superfine powder of this comparative example is different in that the amount of the tea citrus seed powder in the composite tea citrus superfine powder of this comparative example is greatly increased. The specific raw material components and effects are compared, as shown in Table 1.

[0085] Comparative Example 4:

[0086] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that: in Comparative Example 4, the tea-branch citrus fruit body is not subjected to high-voltage pulse electric field treatment and pressure difference puffing treatment, and only vacuum freeze-drying treatment is adopted, that is, pre-freezing at -25°C for 5h, and then drying at -25°C under vacuum conditions for 40h. The specific treatment methods and effects are compared, as shown in Table 1.

[0087] Comparative Example 5:

[0088] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that: in Comparative Example 5, the tea-branch citrus fruit body is not subjected to pressure difference puffing treatment, but is only subjected to high-voltage pulse electric field treatment for 10 minutes and pre-freezing at -25°C for 5 hours, and vacuum freeze-drying at -25°C for 25 hours. The specific treatment methods and effects are compared, as shown in Table 1.

[0089] Comparative Example 6:

[0090] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that: in Comparative Example 6, the tea-branch citrus fruit body is not subjected to high-voltage pulse electric field treatment, but is only subjected to pressure difference puffing treatment for 0.5 h and pre-freezing at -25°C for 5 h, and vacuum freeze-drying at -25°C for 25 h. The specific treatment methods and effects are compared, as shown in Table 1.

[0091] Comparative Example 7:

[0092] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that the electric field intensity of the high-voltage pulse electric field treatment in Comparative Example 7 is 35 kV / cm, and the specific treatment methods and effects are compared, as shown in Table 1.

[0093] Comparative Example 8:

[0094] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, with the only difference being that the electric field intensity of the high-voltage pulse electric field treatment in Comparative Example 11 is 5 kV / cm, and the specific treatment methods and effects are compared, as shown in Table 1.

[0095] Comparative Example 9:

[0096] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that the puffing pressure of the differential pressure puffing treatment in Comparative Example 9 is 0.15 MPa. The specific treatment methods and effects are compared, as shown in Table 1.

[0097] Comparative Example 10:

[0098] A method for preparing a composite tea-branch citrus ultrafine powder is basically the same as the method for preparing the tea-branch citrus ultrafine powder with anti-inflammatory effect in Example 1, except that the puffing pressure of the differential pressure puffing treatment in Comparative Example 12 is 0.02 MPa, and the specific treatment methods and effects are compared, as shown in Table 1.

[0099] Product efficacy test:

[0100] The anti-inflammatory effect of the tea branch orange superfine powder prepared in Example 1 and each comparative example was tested by establishing a rat colitis inflammation model. The specific testing steps include:

[0101] (1) Establishment of colitis rat model: 8 rats were selected as the normal control group, and the remaining rats were treated with trinitrobenzene sulfonic acid (TNBS) to establish the rat ulcerative colitis model;

[0102] (2) The rats in the normal control group were injected with 0.9% sodium chloride injection, and the rats in the other treatment groups were gavaged with the corresponding tea branch orange superfine powder, with a dosage of 10 mL / kg, once a day for 21 consecutive days;

[0103] (3) Measurement of colon length (the measurement of colon length has a certain reference value for the treatment of colitis and symptom assessment, and is an important observation indicator in the study of the treatment of ulcerative colitis): After the last administration, the rats were killed, the colon was dissected and separated, and the colon length was accurately measured. The test results are shown in Table 1 and Figure 5 .

[0104] Table 1 Effects of different raw material components and processing methods on the anti-inflammatory effect of tea branch orange ultrafine powder

[0105]

[0106]

[0107]

[0108] Table 2 Comparison of proinflammatory factors of Example 1 and Comparative Examples 4-6

[0109] Group IL-6 (pg / mL) IL-1β (pg / mL) TNF-α(pg / mL) Normal control group 43.599±17.894 8.556±3.894 69.072±19.012 TNBS control group 55.520±9.110 13.241±2.807 98.510±39.667 Comparative Example 4 51.968±8.853 9.091±2.677 84.755±21.591 Comparative Example 5 53.439±15.55 9.242±3.537 78.714±27.164 Comparative Example 6 44.174±19.236 9.191±2.127 80.133±25.885 Example 1 42.449±16.327 8.849±3.811 64.330±36.045

[0110] Combined with Table 1, Table 2, Figure 3 and Figure 5It can be seen that different raw material components, processing methods and crushing time have a greater impact on the nutrient dissolution rate and antioxidant properties of the tea branch orange whole fruit superfine powder. The specific analysis is as follows: (1) By comparing comparative examples 1, 2, and 3 with embodiment 1, it can be clearly known that different raw material ratios have a greater impact on its anti-inflammatory activity. The tea branch orange whole fruit superfine powder with anti-inflammatory efficacy of the present invention has specific requirements for the ratio of tea branch orange tangerine peel powder, tea branch orange pulp powder, and tea branch orange seed powder. The anti-inflammatory efficacy of the tea branch orange whole fruit superfine powder prepared according to the ratio other than the present invention will be greatly reduced. For example, in comparative example 3, the amount of tea branch orange tangerine peel powder and tea branch orange pulp powder is kept unchanged. When the amount of tea branch orange seed powder is greatly increased, the anti-inflammatory effect is reduced. It can be seen that the amount of tea branch orange seed powder is not the higher the better, and too high will have a counterproductive effect.

[0111] (2) By comparing Comparative Examples 4, 5, and 6 with Example 1, it can be clearly seen that high-voltage pulse electric field + pressure differential puffing + vacuum freeze drying have a synergistic effect, which is of great benefit to the improvement of anti-inflammatory effect and drying efficiency. Vacuum freeze drying alone takes a long time, has low drying efficiency, and consumes a lot of energy. Although the drying efficiency of high-voltage pulse electric field treatment + vacuum freeze drying, or pressure differential puffing treatment + vacuum freeze drying is improved compared with single vacuum freeze drying, it is still far behind Example 1, and only high-voltage pulse electric field or pressure differential puffing combined with vacuum freeze drying is not effective. The promoting effect of the combination on the dissolution rate of biologically active substances needs to be improved. Only by using high-voltage pulse electric field + pressure difference puffing + vacuum freeze drying at the same time can the dissolution of biologically active substances be promoted to the maximum extent, so that the antioxidant activity can be optimized, the anti-inflammatory effect can be optimized, and the drying efficiency can be greatly improved. While saving energy and reducing consumption, the quality of the product can also be improved; in addition, ordinary crushing, crushing time that is too short or too long will affect the product's antioxidant properties and active substance dissolution rate. The present invention uses low-temperature ultrafine crushing and optimized crushing time to obtain the best antioxidant activity and active substance dissolution rate.

[0112] (3) By comparing Comparative Examples 7, 8, 9, and 10 with Example 1, it can be clearly seen that an electric field intensity of the high-voltage pulse electric field treatment that is too high or too low, and an expansion pressure of the differential pressure expansion treatment that is too high or too low are not conducive to the dissolution and retention of biologically active ingredients. An electric field intensity greater than 30 kV / cm will cause loss of pigments, anti-inflammatory ingredients, and nutrients, and the anti-inflammatory effect will also be reduced, and the energy consumption will be higher, resulting in unnecessary waste; an electric field intensity lower than 10 kV / cm will cause insufficient dissolution of anti-inflammatory ingredients and weakened drying effects. An expansion pressure greater than 0.1 MPa will cause loss of anti-inflammatory ingredients, resulting in reduced anti-inflammatory effects, loss of nutrients, and destruction of pigments; an expansion pressure lower than 0.05 MPa will cause insufficient dissolution of anti-inflammatory ingredients and reduced drying efficiency. The pigment destruction can be seen from Figure 3It is clearly seen that Example 1 has bright color, while Comparative Examples 7 and 9 are white, especially Comparative Example 9 is more obviously white, indicating that higher puffing pressure has greater destructive power on pigments. Figure 5 The anti-inflammatory test results show that the anti-inflammatory effect of the tea branch orange superfine powder obtained after the treatment of comparative examples 7 and 9 is significantly lower than that of example 1. Figure 5 TNBS is a positive control for colitis without any anti-inflammatory treatment. Compared with TNBS in Example 1, the colon length increased by 25%, which also verifies the superior anti-inflammatory effect of Example 1.

[0113] (4) Proinflammatory factors are a class of small molecule polypeptide substances synthesized and secreted by the body's immune and non-immune cells. These factors participate in inflammatory reactions through various signal pathways, promote the recruitment, activation and proliferation of immune cells, and increase vascular dilation and permeability at the site of inflammation, thereby inducing inflammatory reactions. The present invention also detected the proinflammatory factors of Example 1, Comparative Examples 4-6, the normal group and the TNBS control group in the rat colitis inflammation model test. The results are shown in Table 2. Table 2 is a comparison table of the proinflammatory factors of Example 1 of the present invention and Comparative Examples 4-6. It can be seen from Table 2 that the proinflammatory factors of the group treated with Example 1 of the present invention are significantly lower than those of Comparative Examples 4-6 and the TNBS control group.

[0114] (5) Figure 4 It is a comparison chart of total phenol content, vitamin C content, and antioxidant capacity of Example 1 of the present invention and Comparative Examples 1, 2, 3, 7, and 9. It can be seen from the figure that Example 1 better promotes the dissolution of total phenol and vitamin C, significantly improves the scavenging rates of ABTS and DPPH free radicals, and has a stronger antioxidant capacity.

[0115] (6) Through single factor experiments, it was found that the optimal time for low-temperature ultrafine grinding was 10 minutes, and the average particle size could reach 10 μm to 20 μm, which could significantly improve the dissolution rate of nutrients. Compared with ordinary grinding, the low-temperature ultrafine grinding used in the present invention increased the total flavonoid content by 16%, the total phenol content by 13%, the vitamin C content by 17%, and the antioxidant activity by ABTS clearance rate and DPPH clearance rate by 11% and 19%, respectively.

[0116] The above is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as above in the preferred embodiment, it is not used to limit the present invention. Any technician familiar with the art can make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above without departing from the spirit and technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention, still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A tea branch orange superfine powder with anti-inflammatory effect, characterized in that: The tea branch orange superfine powder includes the following raw material components by weight: 15 to 30 portions of tea branch orange peel powder, The content of nobiletin in the tea branch orange peel powder is 3.61 mg / g to 4.32 mg / g; 30 to 50 portions of tea branch orange pulp powder, The content of hesperidin in the tea branch orange pulp powder is 8.05mg / g to 9.65mg / g; 4 to 8 portions of tea branch citrus fruit seed powder, The content of limonin in the tea branch citrus fruit seed powder is 18.12 mg / g to 19.05 mg / g.

2. The tea branch orange superfine powder with anti-inflammatory effect according to claim 1, characterized in that The tea branch orange superfine powder includes the following raw material components by weight: 15 portions of tea branch orange peel powder, 30 portions of tea branch orange pulp powder, 4 parts of Citrus aurantium seed powder.

3. The tea branch orange superfine powder with anti-inflammatory effect according to claim 1, characterized in that The tea branch orange superfine powder includes the following raw material components by weight: 30 portions of tea branch orange peel powder, 50 portions of tea branch orange pulp powder, 8 parts of Citrus aurantium seed powder.

4. The tea branch orange superfine powder with anti-inflammatory effect according to any one of claims 1 to 3, characterized in that: Tea-branch oranges are big red oranges, which are harvested from November to December every year.

5. The tea branch orange superfine powder with anti-inflammatory effect according to claim 4, characterized in that: The tea-twig citrus tangerine peel powder is prepared from tea-twig citrus tangerine peel that is more than three years old.

6. The tea branch orange superfine powder with anti-inflammatory effect according to claim 4, characterized in that The pulp used in the tea-branch citrus pulp powder includes pulp body, citrus skin and citrus pith.

7. A method for preparing the tea branch orange ultrafine powder with anti-inflammatory effect as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: S1, separating the peel and the fruit body of the tea-branch orange, the peel is used to make the tea-branch orange tangerine peel, and the fruit body is subjected to a high-voltage pulse electric field treatment, the electric field strength is 10kV / cm~30kV / cm, and the treatment time is 10min~30min; S2, subjecting the tea-branch citrus fruit body treated in step S1 to differential pressure puffing treatment, wherein the puffing pressure is 0.05MPa-0.1MPa, the puffing temperature is 35°C-40°C, and the puffing time is 0.5h-1h; S3, subjecting the tea-branch orange fruit body treated in step S2 to vacuum freeze-drying; S4, separating the pulp and seeds of the tea-branch citrus fruit body treated in step S3 to obtain tea-branch citrus pulp and tea-branch citrus seeds; S5, respectively grinding the tea-branch orange peel, tea-branch orange pulp and tea-branch orange seeds by low-temperature dry ultrafine grinding technology to obtain tea-branch orange peel powder, tea-branch orange pulp powder and tea-branch orange seed powder; S6. Compound the tangerine peel powder, tangerine pulp powder and tangerine seed powder according to a certain proportion to obtain tangerine ultrafine powder with anti-inflammatory effect.

8. The method for preparing the superfine powder of Citrus aurantium with anti-inflammatory effect according to claim 7, characterized in that: In step S3, the vacuum freeze-drying treatment is to pre-freeze the tea-branch citrus fruit body at -25°C to -30°C for 3h to 5h, and then dry it under vacuum conditions at -25°C to -20°C for 20h to 25h.

9. The method for preparing the tea branch orange superfine powder with anti-inflammatory effect according to claim 8, characterized in that: In step S5, the pulverizing temperature is 0°C to 4°C, and the pulverizing time is 10 min to 30 min.

10. The method for preparing the tea branch orange superfine powder with anti-inflammatory effect according to any one of claims 7 to 9, characterized in that: In step S6, the tea branch orange ultrafine powder is irradiated and sterilized for 30 minutes to 40 minutes, and then the sterilized ultrafine powder is vacuum-packed with aluminum foil or tin foil.

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